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Dissertations / Theses on the topic 'Proteine s1'

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1

Hahn, Véronique. "Utilisation d'anticorps polyclonaux et monoclonaux pour l'etude de la structure et de la fonction de la proteine s1 du ribosome d'escherichia coli." Université Louis Pasteur (Strasbourg) (1971-2008), 1987. http://www.theses.fr/1987STR13154.

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2

Zoulim, Fabien. "Signification de l'expression des proteines pre-s1 dans le serum et les cellules mononucleees du sang au cours des infections chroniques dues au virus de l'hepatite b." Lyon 1, 1990. http://www.theses.fr/1990LYO1M151.

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3

HU, ROUH-MEI. "Etude de la specificite de l'endoribonuclease regb du bacteriophage t4 : influence de la sequence et de la structure de l'arn : role de la proteine ribosomique s1." Paris 11, 1998. http://www.theses.fr/1998PA112320.

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L'endoribonuclease regb du bacteriophage t4 coupe au milieu des motifs ggag des arnm. Regb purifiee est tres peu active in vitro. Son activite est stimulee environ 100 fois par la proteine ribosomique s1. In vivo, s1 est responsable de l'inactivation fonctionnelle et chimique des arnm precoces de t4 et egalement de la maturation des longs arnm precoces de t4. In vivo, tous les motifs ggag ne sont pas coupes par regb. Dans cette these, je montre que la sensibilite et la resistance a regb observees apres une infection par t4 peuvent etre reproduites in vivo dans les cellules d'e. Coli non-infect
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4

Hartmann, Marie-Louise. "Application des anticorps monoclonaux a l'etude de quelques proteines de sous-unite 30s du ribosome d'e. Coli." Université Louis Pasteur (Strasbourg) (1971-2008), 1987. http://www.theses.fr/1987STR13199.

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Nous avons prepare des anticorps monoclonaux diriges contre des proteines de la sous-unite 30s du ribosome d'e. Coli en vue de leur utilisation comme sondes fonctionnelles et structurales. Nous presentons d'abord une technique de fractionnement des proteines de la sous -unite 30s du ribosome d'e. Coli par chromatographie en phase inverse sur un systeme fplc. Puis, nous decrivons l'obtention et la caraterisation d'anticorps monoclonaux diriges contre quelques proteines de la sous-unite 30s: les proteines s1, s4, s12 et s17. Ces anticorps ont permis de montrer que des modifications des condition
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5

Thomas, Franck. "Expression des gènes rpl23, rpl2, rps19 et rps19' du génome chloroplastique d'épinard : identification des produits de quelques gènes de protéines ribosomiques." Grenoble 1, 1987. http://www.theses.fr/1987GRE10171.

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Le genome chloroplastique d'epinard est constitue d'une molecule d'adn circulaire (140 kbp) organisee en 4 regions: une sequence unique (lsc) et une petite sequence unique (ssc) separees par deux regions inversees repetees (ira et irb). L'expression des genes rp12, rps19 et rps19' est etudie. Les techniques de clonage et de cartographie a la nuclease s1 ont peris de montrer que le gene rps19' n'est pas exprime "in vivo" dans le chloroplaste en raison de la co-transcription sur l'autre brin des genes psba et trn h-gug. Les genes rp12 et rps19 codent respectivement pour les proteines ribosomique
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6

Bisaglia, Marco. "Etude fonctionnelle et structurale de deux protéines impliquées dans le métabolisme des ARN messagers." Palaiseau, Ecole polytechnique, 2002. http://www.theses.fr/2002EPXX0018.

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7

Sinor, Cleve Ray-Dean. "Xenopus laevis Ribosomal Protein S1: Evidence for Regulation at the Transcriptional Level." W&M ScholarWorks, 1994. https://scholarworks.wm.edu/etd/1539625876.

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8

Marenna, Alessandra. "Staphylococcus aureus protein S1, an RNA chaperone involved in translation initiation and sRNA regulation." Thesis, Strasbourg, 2017. http://www.theses.fr/2017STRAJ080/document.

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Bien que l'initiation de la traduction soit un processus conservé entre les bactéries, nous avons montré que le mécanisme par lequel les ARNm structurés sont reconnus et adaptés sur le ribosome diffère chez Staphylococcus aureus, un micro-organisme avec un bas taux de G+C et chez Escherichia coli. Une particularité du ribosome de S. aureus est l'absence de la protéine ribosomale S1, qui non seulement est plus courte que celle de E. coli mais qui possède également une organisation distincte des domaines. Mes expériences suggèrent que la protéine S1 (SauS1) favorise spécifiquement l'initiation d
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9

Knoetze, Adrian David. "Investigation into the variation of infectious bronchitis virus serotypes in KwaZulu-Natal poultry flocks." Diss., University of Pretoria, 2013. http://hdl.handle.net/2263/40700.

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Infectious bronchitis virus (IBV) is a member of family Coronaviridae and is classified into group 3 of the Coronaviruses. The virus is a single-stranded positive-sense RNA virus with a genome of 27kbp. IBV is a highly infectious disease of chickens that results in high morbidity with moderate to severe mortality depending on the strain involved, age of the birds, and immune status of the chickens. Multiple worldwide investigations indicate that differentiation within the S1 glycoprotein gene can lead to serotype variation within the IBV species. In this study 46 isolates collected over two ye
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10

Duval, Mélodie. "La protéine ribosomique S1 d'Escherichia coli au carrefour de la traduction et de la régulation de l'expression des gènes." Thesis, Strasbourg, 2015. http://www.theses.fr/2015STRAJ065.

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La traduction est une étape clef de l’expression des gènes, et mon travail a consisté à étudier l’implication de la protéine ribosomique S1 d’Escherichia coli dans l’initiation de la traduction des ARNm structurés. Mes résultats montrent que 1) S1 est requise pour la formation du complexe d’initiation des ARNm portant une séquence SD faible et/ou des structures stables, 2) elle est dotée d’une activité chaperonne, débobinant les ARNm afin de les placer dans le canal de décodage ; et 3) le ribosome favorise son action. Par la suite, j’ai montré un rôle inattendu de S1 dans la régulation post-tr
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11

Hahn, Véronique. "Utilisation d'anticorps polyclonaux et monoclonaux pour l'étude de la structure et de la fonction de la protéine S1 du ribosome d'Escherichia coli." Grenoble 2 : ANRT, 1987. http://catalogue.bnf.fr/ark:/12148/cb376057115.

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12

Liu, Catherine Heung Luen. "Expression of an EF-1a like rat cDNA, S1, in Escherichia coli and production of a rabbit polyclonal antiserum to the recombinant protein." Thesis, McGill University, 1992. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=61334.

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A previously identified rat cDNA (S1) that shares 78% nucleotide homology and a predicted 92% amino acid sequence homology with human EF-1$ alpha$ was used to express S1 in E. coli and to generate a polyclonal antibody to pS1. A recombinant plasmid pGEX-2T-S1 was constructed, containing the glutathione S-transferase gene. The expressed fusion protein was purified and digested with thrombin to produce a recombinant S1 protein (rpS1) containing slightly modified N-terminus. Purified rpS1 was used to raise a rabbit antiserum which recognized rpS1 on immunoblots. A polyclonal antiserum to EF-1$ al
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13

Finger, Paula Fonseca. "Expressão da glicoproteína S1 do vírus da bronquite infecciosa das galinhas em sistemas procarioto e eucarioto para utilização em imunodiagnóstico." Universidade Federal de Pelotas, 2011. http://repositorio.ufpel.edu.br/handle/ri/2473.

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Made available in DSpace on 2014-08-20T14:37:49Z (GMT). No. of bitstreams: 1 dissertacao_paula_finger.pdf: 729110 bytes, checksum: fe15bb22f0abb92bf1b2938fd6dcdfc9 (MD5) Previous issue date: 2011-12-19<br>The chickens infectious bronchitis (IB) is a highly contagious viral disease which causes predominantly respiratory lesions manifested clinically and invariably by sneezing and tracheo-bronchial rales, which may lead to more severe signs, with a decrease in fertility and reduction of eggs production. The infectious bronchitis virus (IBV) encodes four major structural proteins: N (nucleoc
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14

Rossa, Giselle Ayres Razera. "Diversidade molecular dos genes codificadores das proteínas não-estruturais Nsp2 e protease Papaína-like e da proteína estrutural S1 de amostras brasileiras do Coronavírus aviário." Universidade de São Paulo, 2014. http://www.teses.usp.br/teses/disponiveis/10/10134/tde-30032015-142540/.

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Coronavírus, incluindo-se o Coronavírus aviário (ACoV), possuem o maior genoma composto por RNA conhecido entre os vírus. Aproximadamente dois terços desse genoma codificam proteínas não estruturais (Nsps), cujas funções parecem estar associadas à replicação e patogênese viral. Até o momento, esses alvos têm sido pouco explorados quanto a sua diversidade em diferentes linhagens de ACoV. O presente estudo teve como objetivo investigar a diversidade dos genes codificadores das proteínas não estruturais Nsp2 e protease Papaína-like (Plpro), utilizando-se linhagens brasileiras de ACoV. Para tanto,
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15

Tsao, Theresa Tsun-Hui. "Towards the development of transgenic banana bunchy top virus (BBTV)-resistant banana plants : interference with replication." Queensland University of Technology, 2008. http://eprints.qut.edu.au/17031/.

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Banana bunchy top virus (BBTV) causes one of the most devastating diseases of banana. Transgenic virus resistance is now considered one of the most promising strategies to control BBTV. Pathogen-derived resistance (PDR) strategies have been applied successfully to generate plants that are resistant to numerous different viruses, primarily against those viruses with RNA genomes. BBTV is a circular, single-stranded (css) DNA virus of the family Nanoviridae, which is closely related to the family Geminiviridae. Although there are some successful examples of PDR against geminiviruses, PDR against
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16

Huang, Chih-You, and 黃智佑. "Expression of immunoregulatory proteins and avian IBV S1 subunit." Thesis, 2012. http://ndltd.ncl.edu.tw/handle/75086513930097396569.

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碩士<br>國立中興大學<br>微生物暨公共衛生學研究所<br>100<br>The cytokines are naturally derived proteins that play important roles in controlling and promoting immune responses, and therefore may have potential to serve as good vaccine adjuvants. Avian infectious bronchitis (IB) is an important avian disease and its causative agent is avian infectious bronchitis virus (IBV). Many different IBV serotypes have been recognized with little cross protection, rendering great economic losses due to vaccination failure. In this study, we expressed fusion cytokines and S1 subunit of IBV for the future vaccine usage. To pro
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17

Erraguntla, Mythili. "Genetic Analysis And Biochemical Activities Of β Protein : A Component Of Bacteriophage λ General Genetic Recombination". Thesis, 1995. http://etd.iisc.ernet.in/handle/2005/1906.

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18

"Understanding the Role of Human TRPV1 S1-S4 Membrane Domain in Temperature and Ligand Activation." Doctoral diss., 2019. http://hdl.handle.net/2286/R.I.55677.

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abstract: Transient receptor potential vanilloid member 1 (TRPV1) is a membrane protein ion channel that functions as a heat and capsaicin receptor. In addition to activation by hot temperature and vanilloid compounds such as capsaicin, TRPV1 is modulated by various stimuli including acidic pH, endogenous lipids, diverse biological and synthetic chemical ligands, and modulatory proteins. Due to its sensitivity to noxious stimuli such as high temperature and pungent chemicals, there has been significant evidence that TRPV1 participates in a variety of human physiological and pathophysiological
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19

Bayrhuber, Monika. "Strukturelle und funktionelle Charakterisierung von dem mitochondrialen Membranprotein Menschlicher Spannungsabhängiger Anionen Kanal (HVDAC) und dem Membranprotein bindenden Conotoxin Conkunitzin-S1 mit Flüssigphasen NMR." Doctoral thesis, 2007. http://hdl.handle.net/11858/00-1735-0000-0006-AC97-F.

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20

Huang, Lih-Yi, and 黃立怡. "Cloning and analysis of the Hepatitis B virus pre-S1 and human placenta alkaline phosphatase chimeric proteins." Thesis, 1998. http://ndltd.ncl.edu.tw/handle/08379404943783330940.

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碩士<br>國立臺灣大學<br>微生物學研究所<br>86<br>Hepatitis B viral infection, like other viruses, it should first bind to the r eceptor(s) on the target cell surface by its envelope protein and the membrane fusion will occur between HBV and target cell. After entering the cell, it wi ll begin its replication. So successful infection of HBV depends on whether HB V could bind the receptor(s) on the target cell. It is not yet clear what is H BV receptor. There are data showing that the HBV large surface antigen (L) is invo
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21

Tsai, Cheng-Hsuan, and 蔡承軒. "Production and analysis of monoclonal antibodies against the S1 protein of canine coronavirus and Lig.B protein of Leptospira interrogans and epitope mapping of VP2 protein of canine parvovirus." Thesis, 2015. http://ndltd.ncl.edu.tw/handle/78233001890985839382.

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碩士<br>國立中興大學<br>分子生物學研究所<br>103<br>Canine coronavirus (CCoV), Canine parvovirus (CPV) and Leptospira interrogans, All are common pathogens for canine. CCoV and CPV can cause gastrointestinal disease of dogs. CPV will lead to canine respiratory disease and the Leptospira can affect liver and kidney function and cause internal bleeding and gastrointestinal diseases according to their different serotypes. CCoV enters the cell utilize spike protein. CPV VP2 protein is important role into host cells. Leptospirosis is use Lig.A and Lig.B proteins into host cells. In this study, we product protein
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22

Bayrhuber, Monika [Verfasser]. "Structural and functional characterisation of the mitochondrial membrane protein human voltage-dependent anion channel (HVDAC) and the membrane protein-targeting conotoxin conkunitzin-S1 by solution NMR / vorgelegt von Monika Bayrhuber." 2007. http://d-nb.info/986911658/34.

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23

Yom, Heng-Cherl. "Genetic engineering of milk proteins in transgenic animals expression of bovine [alpha]S1 casein under the control of mouse mammary tumor virus promoter in the milk of transgenic mice /." 1991. http://catalog.hathitrust.org/api/volumes/oclc/24478268.html.

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