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Dissertations / Theses on the topic 'Proteins Cellular signal transduction'

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Published: 4 June 2021

Last updated: 1 February 2022

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1

Brownlie, Zoe. "Regulation of signal transduction by RGS4." Connect to e-thesis, 2007. http://theses.gla.ac.uk/124/.

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Thesis (Ph.D.) - University of Glasgow, 2007.
Ph.D. thesis submitted to the Division of Biochemistry and Molecular Biology, Institute of Biomedical and Life Sciences, University of Glasgow, 2007. Includes bibliographical references.

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2

Rogers, Laura Ann. "Membranes as a hub for cellular signaling /." Access full-text from WCMC, 2007. http://proquest.umi.com/pqdweb?did=1481668281&sid=2&Fmt=2&clientId=8424&RQT=309&VName=PQD.

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3

Pat, Betty Kila. "Signal transduction pathways in renal fibrosis /." St. Lucia, Qld, 2003. http://www.library.uq.edu.au/pdfserve.php?image=thesisabs/absthe17739.pdf.

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4

Lo, Kin Ho. "Activation of signal transducer and activator of transcription 3 (STAT3) by G[alpha]16 and G[alpha]14 via a c-Src/JAK-and ERK-dependent mechanism /." View abstract or full-text, 2004. http://library.ust.hk/cgi/db/thesis.pl?BICH%202004%20LO.

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Thesis (M. Phil.)--Hong Kong University of Science and Technology, 2004.
Includes bibliographical references (leaves 92-111). Also available in electronic version. Access restricted to campus users.

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5

Liu, Wei. "Role of axin in TGF-[beta] signaling pathway and characterization of axin mutant proteins in axinF̳u̳ mice /." View abstract or full-text, 2006. http://library.ust.hk/cgi/db/thesis.pl?BICH%202006%20LIUW.

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6

Ma, Chun-Wai. "Aboav-Weaire law in complex network and its applications in bioinformatics /." View abstract or full-text, 2005. http://library.ust.hk/cgi/db/thesis.pl?PHYS%202005%20MA.

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7

Chen, Yujie, and 陈宇杰. "Structural and functional studies of human APPL1-APPL2 BAR-PH heterodimer, APPL2 BAR-PH homodimer, and lanthionine synthetase component C-like protein 2." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2012. http://hdl.handle.net/10722/197138.

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APPL BAR-PH heterodimer and APPL2 BAR-PH homodimer The APPL (Adaptor protein containing PH domain, PTB domain and Leucine zipper) family are adaptor proteins with only two isoforms, APPL1 and APPL2. They bind to early endosomes with a small GTPase, Rab5, and mediate the interactions between various receptors and downstream signaling components, thus functioning in many signaling pathways evoked by adiponectin, insulin, FSH, EGF, and so on. However, evidences have shown APPL1 and APPL2 should perform some opposite functions, which cannot be simply explained by the functional differences attributed to their PTB domains. We hypothesize that the heterodimerization of APPL1 and APPL2’s BAR domains may account for their opposing functions. The crystal structure of APPL BAR-PH heterodimer was solved to resolution 2.8 Å. Its overall structure exhibits crescent shape with a larger curvature radius of 76 Å, compared to 55 Å of the APPL1 BAR-PH homodimer. And the crystal structure APPL2 BAR-PH homodimer was solve to resolution 3.3 Å. The overall structure of APPL2 BAR-PH homodimer is very similar to that of APPL BAR-PH heterodimer, but shows greater difference in curvature to the APPL1 BAR-PH homodimer structure. The concave side of APPL BAR-PH heterodimer and APPL2 BAR-PH homodimer all possess less positive charge than the APPL1 BAR-PH homodimer. Structural analysis reveals that leucine patches at the dimer interface may account for the formation of dimeric curve with certain curvature. Consequently, APPL2 BAR is able to enforce the curvature reduction to APPL1 BAR upon heterodimerization. In conclusion, the alterations of curvature and electrostasis are responsible for the modulation of endosome binding specificity and can elucidate the opposite roles of APPL1 and APPL2. LanCL2 LanCL2 is a member of Lanthionine synthetase component C-like family. In human, LanCL2 binds to lanthionine derivatives and glutathione, participating in keeping intracellular reducing state. By binding to absiscic acid (ABA), LanCL2 is indispensible for the ABA-stimulated adipogenesis, insulin release, glucose homeostasis, and inflammatory response. It is also implicated in anticancer drug resistance. All these functions underscore the importance of LanCL2 in the diseases like diabetes, inflammation, and cancer. The crystal structure of LanCL2 was solved to resolution 1.8 Å. The overall structure displays canonical double-layer α-barrel. The major differences from LanCL1 lay in the loops on the barrel top, which are implicated in various substrate bindings. A zinc-coordinating pocket was found among the loops, with conserved amino acid residues of distinct conformation. The electrostatic surface shows remarkable differences compared to that of LanCL1, suggesting that it may contribute to distinct substrate binding profile. Future implications APPL proteins and LanCL proteins are all involved in the regulation of metabolism, such as glucose uptake, fatty acid oxidation, and insulin secretion, and play roles in diseases like obesity and type 2 diabete. Structural and functional studies of these proteins can provide insights into the molecular mechanisms and clues for related therapeutic approaches.
published_or_final_version
Physiology
Doctoral
Doctor of Philosophy

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8

Lupi, Rosita. "Characterization of post translational modification of heterotrimeric G proteins." Thesis, Open University, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.343748.

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9

Philips, Brian John. "Protein interactions with the catechol estrogens 4-hydroxyestrone and 4-hydroxyestradiol in mouse tissue lysate : binding and metabolism studies /." free to MU campus, to others for purchase, 2001. http://wwwlib.umi.com/cr/mo/fullcit?p3036851.

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10

Alexander, Roger Parker. "Evolutionary Genomics of Methyl-accepting Chemotaxis Proteins." Diss., Georgia Institute of Technology, 2007. http://hdl.handle.net/1853/19860.

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The general goal of this project was to use computational biology to understand signal transduction mechanisms in prokaryotes. Its specific focus was to characterize the cytoplasmic domain of methyl-accepting chemotaxis proteins (MCP_CD), a protein domain central to the function of chemotaxis, the most complex signaling network in prokaryotes. Chemotaxis enables cells to sense and respond to multiple external and internal stimuli by actively navigating to an optimal environment. MCP_CD is a central part of this circuit, but its coiled coil structure is difficult to analyze using traditional tools of computational biology. In this project, a new method for analysis of the domain was developed and used to gain insight into its function and evolution. Research advance 1: Characterization of the MCP_CD protein domain. Before this work, MCP_CD was known to have two distinct functional regions: the signaling region that activates the histidine kinase CheA and the methylation region where adaptation enzymes CheB and CheR store information about recent stimuli. The result of this project is classification of ~2000 MCP_CDs into twelve subfamilies. The unique mechanism of evolution of the domain has been clarified and precise boundaries of the adaptation and signaling regions determined. A new functional region, the flexible bundle subdomain, was identified and its contribution to the signaling mechanism elucidated by analysis of conserved sequence features. Conserved and variable sequence features in the adaptation and signaling subdomains led to a better understanding of the evolutionary history of the adaptation mechanism and of alternative higher-order arrangements of receptors within the membrane. Research advance 2: Development of a sensor / kinase correlation algorithm to couple diverse MCP_CD and kinase subfamilies. The receptor diversity discovered in this work is complemented by diversity in the kinases with which they interact. In this work, an algorithm was developed to associate receptor / kinase pairs which facilitated understanding of the function and evolution of chemotaxis. Research advance 3: Development of Cheops, a database of chemotaxis pathways. The Cheops (Chemotaxis operons) database presents the results of the sensor / kinase correlation algorithm and the information about receptor and kinase diversity in an integrated and intuitive way.

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11

Wu, Hoi Ti. "Regulation of the pro-survival AKT signaling cascade : roles of receptor tyrosine kinases, G proteins, and their crosstalks /." View abstract or full-text, 2006. http://library.ust.hk/cgi/db/thesis.pl?BICH%202006%20WU.

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12

Ip, Koon-ching. "Role of G[alpha]-interacting protein (GAIP) in modulation of MAPK pathways /." View abstract or full-text, 2008. http://library.ust.hk/cgi/db/thesis.pl?BICH%202008%20IP.

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13

Wen, Wenyu. "Structural characterization of proteins involved in cellular signaling and trafficking /." View abstract or full-text, 2008. http://library.ust.hk/cgi/db/thesis.pl?BICH%202008%20WEN.

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14

Hu, Xiao Wooten Marie W. "Analysis of P62-UBA interacting proteins." Auburn, Ala., 2006. http://repo.lib.auburn.edu/2006%20Spring/master's/HU_XIAO_49.pdf.

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15

Toribio, Isaías Luis Daniel. "Signal Transduction proteins in Streptococcus pneumoniae and Vibrio cholerae." Doctoral thesis, Universitat de Barcelona, 2019. http://hdl.handle.net/10803/668345.

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The aim of this project is to structurally characterize proteins involved in bacterial signal transduction systems by applying X-ray crystallography. Bacteria use signal transduction systems to react in response to any environmental changes detected. Bacterial signal transduction is divided into two categories, one- component systems and two-component systems. Two-component systems are composed by a Response Regulator (RR) and a Histidine Kinase (HK); the Histidine Kinase auto phosphorylates an inner domain, and soon after, it phosphorylates the receiver domain on the Response Regulator, activating the output domain; usually producing a physiological effect in the cell by activating a specific gene. While in one-component systems, one protein has both, a sensory and an output domain. An example of the one-component systems would be ToxR, and an example of the two- component systems would be ComD-ComE. Bacterial transformation is a type of Horizontal Gene Transfer (HGT), which is a rapid evolutive mechanism in which entire genes can be transferred among bacterial cells. HGT is commonly deemed responsible for the appearance of antibiotic resistance, virulence factors and serotype switching. Competence for genetic transformation in Streptococcus pneumoniae is a transient physiological state whose development is coordinated by a peptide pheromone (Competence Simulating Peptide or CSP) and its receptor, which activates transcription of two downstream genes, comX and comW, and 15 other “early” genes. ComD (HK) and ComE (RR) are involved in the quorum-signaling pathway that synthesizes the CSP. They help modulate the mechanism in which bacterial transformation occurs, by allowing the inclusion of naked DNA from the environment. We have successfully formed the binary (ComE+DNA) and ternary (ComD+ComE+DNA) complexes and characterized them in the attempt of obtaining crystals to solve their 3-D structure. On the other hand, to elaborate on one-component systems, like ToxR we can discuss cholera. Cholera is caused by the causative agent Vibrio cholerae. It is estimated that there are from 1.3 to 4.0 million cases out of which up to 143,000 result in cholera deaths annually. After ingesting the V. cholerae, it travels to the small intestine colonizing it and producing the cholera toxin. ToxR is a membrane-localized transcription factor that regulates the toxT promoter. The activation of the toxT promoter triggers the virulence cascade that leads to the secretion of toxin-coregulated pilus (TCP) and the expression of cholera toxin (CTX). In recent years, our lab solved the crystal structure of the cytoplasmic domain of ToxR+20DNA, proposing molecular interactions between ToxR and the toxT promoter (Simone Pieretti’s PhD Thesis). In this study, we want to determine the crystallographic structure of three mutants of the cytoplasmic domain of ToxR bound to the toxT promoter. According to biochemical data from our collaborator (Professor Eric Krukonis, from the university of Detroit), these mutations down regulate the activation of ToxR and we aim to analyze the structural changes that these mutants suppose. Using X-ray crystallography we solved the structure of three complexes; ToxRQ78A+20DNA, ToxRS81A+20DNA and ToxRP101A+20DNA at 2.55, 2.95 and 2.95 Å resolution, respectively. We have compared the final mutant structures with the wildtype, unveiling how the structural changes result in the decrease in activation of the toxT promoter.

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16

Kim, Jae-Hwan. "Involvement of G protein and protein tyrosine kinase signal transduction in pig oocyte activation /." free to MU campus, to others for purchase, 1999. http://wwwlib.umi.com/cr/mo/fullcit?p9946271.

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17

Wong, Wai-lap. "Signal transduction pathways of ret receptor tuyrosine kinase." Hong Kong : University of Hong Kong, 2000. http://sunzi.lib.hku.hk/hkuto/record.jsp?B22786478.

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18

王偉立 and Wai-lap Wong. "Signal transduction pathways of ret receptor tuyrosine kinase." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2000. http://hub.hku.hk/bib/B31225354.

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19

Legate, Kyle R. Andrews D. W. "Characterization of the beta-subunit of the mammalian SRP receptor and its role in assembly of the SRP receptor /." *McMaster only, 2003.

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20

Deng, Yu. "Regulation of mammalian STE20-like kinase (MST2) by phosphorylation/dephosphorylation, proteolysis and association with HSP90 during apoptosis /." View Abstract or Full-Text, 2003. http://library.ust.hk/cgi/db/thesis.pl?BICH%202003%20DENG.

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Thesis (Ph. D.)--Hong Kong University of Science and Technology, 2003.
Includes bibliographical references (leaves 148-164). Also available in electronic version. Access restricted to campus users.

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21

Kho, Yik Shing. "Identification and characterization of a novel family of transmembrane and coiled-coil proteins /." View abstract or full-text, 2008. http://library.ust.hk/cgi/db/thesis.pl?BICH%202008%20KHO.

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22

Tsui, Michelle Grace, and 徐善婷. "PDZD2, a candidate for brachydactyly type A1, encodes a secreted protein that negatively modulates hedgehog signaling." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2014. http://hdl.handle.net/10722/209211.

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Hedgehog (Hh) is an important morphogen that dictates tissue patterning during embryonic development. Recent studies showed that mutation in Indian Hedgehog(IHH)resulted in Brachydactyly type A1(BDA1);however, the disease pathogenesis in patients without IHH mutation remained unknown. PDZD2 is a multi-PDZ domain-containing protein of unknown function in early development. Human PDZD2 is mapped to chromosome 5p13.2, which co-localizes with the disease-associated gene in a family of BDA1 patients, suggesting involvement of PDZD2 in limb development. In situ hybridization revealed that Pdzd2 was expressed in the distal mesenchyme partially overlapping with Shh expressionin mouse limb bud. During digit patterning, Pdzd2 was expressed in the interzone regions that flanked the Ihh/Gli1-expressing phalanx condensation. Moreover,Pdzd2 was expressed in the paraxial mesoderm adjacent to the differentiating neural tube. Pdzd2expression in various Hh-active tissues in mouse and chicken suggested an evolutionary conserved role of PDZD2 in modulating general Hh signaling during early development. Interestingly, PDZD2 protein was detected at the neural tube away from its site of synthesis, suggesting a non-cell autonomous role of PDZD2 possibly via its secreted form, sPDZD2. Functional studies showed that overexpression of sPDZD2 in the chicken neural tube leads to down-regulation ofNKX2.2andOLIG2expression.sPDZD2 was shown to counteract the ectopic NKX2.2 expression induced by long-range signaling of ectopic HH. Consistently, sPDZD2exhibited an inhibitory effect on SHH-induced reporter activity in a Gli-luciferase cell line. For in vivo analysis, a transgenic mouse line carrying a floxed Pdzd2 allele (Pdzd2fl) was generated to ablate Pdzd2 expression.Pdzd2+/fl mice were crossed with Protamine-cre to generate the null allele (Pdzd2-). However, heterozygous intercross yielded no homozygous mutant as early as E9.5, suggesting early embryonic lethality. Thus, conditional Pdzd2 knock-out in the limb was pursuedusingPrx1-cre.However, no significant perturbation of Hh signaling was observed in Pdzd2fl/fl:Prx1-cre limb buds, which might be due to incomplete knock-out of Pdzd2, or functional redundancy among Hh modulators. To study the relevance of Pdzd2in the development of BDA1, Pdzd2-/fl mouse was crossed with the BDA1 mouseIhhE95K/E95Kto study the effect of reducing Pdzd2expression under defective Hh signaling. Preliminary analysis of the Pdzd2+/-, Ihh+/E95K compound mutants showed more severe phenotypes comparing with IhhE95K/E95K. These included delayed limb development and further diffusion ofGli1expression in the digits, suggestive of a direct involvement of Pdzd2in BDA1. It was speculated that Pdzd2negatively modulated Ihh signaling and restricted Hh signals from entering the interzone, which was required for normal digit patterning. Depletion of Pdzd2might result in an expansion of Ihh signaling into the interzone, leading to the BDA1phenotypessimilar to the current BDA1 disease model proposed forIhhE95Kmutation. Taken together, my study revealed the novel expression of Pdzd2in close proximity to multiple Hh-active tissues during early development and provided the first evidence that PDZD2/sPDZD2 is a negative modulator of general Hh signaling. These data strongly supported PDZD2as a disease-associated locus in the family of BDA1 patients that do not carry IHHmutations.
published_or_final_version
Biochemistry
Doctoral
Doctor of Philosophy

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23

Braun, David Meyer. "Maize receptor-like protein kinase signal transduction and function /." free to MU campus, to others for purchase, 1997. http://wwwlib.umi.com/cr/mo/fullcit?p9841268.

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24

Chan, Anthony Siu Lung. "Activation of c-jun N-terminal kinase by G protein-coupled receptors and the cross-communication with epidermal growth factor signaling /." View Abstract or Full-Text, 2002. http://library.ust.hk/cgi/db/thesis.pl?BICH%202002%20CHAN.

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Thesis (Ph. D.)--Hong Kong University of Science and Technology, 2002.
Includes bibliographical references (leaves 201-236). Also available in electronic version. Access restricted to campus users.

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25

Lee, Tae Weon. "Regulation of expression of signal transduction cascade elements by G-protein coupled receptors." Thesis, University of Glasgow, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.321070.

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26

Miljan, Erik Armand Jaan. "Molecular and cellular characterization of ganglioside-stimulated protein kinase." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 1999. http://hub.hku.hk/bib/B29957618.

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27

Mohamed, Othman. "Identification of multiple roles for Wnt signaling during mouse development." Thesis, McGill University, 2004. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=85087.

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Signaling molecules play essential roles in communication between cells. Wnt signaling molecules are critical for embryonic development of several organisms. I examined the involvement of Wnt signaling during two major developmental processes, namely embryo implantation and formation of the embryonic body axes. Using RT-PCR analysis, I showed that multiple Wnt genes are expressed in the blastocyst at the time of implantation. Moreover, expression of Wnt 11 requires both estrogen produced by the mother and the uterine environment. Using a transgenic approach, I showed that beta-catenin-regulated transcriptional activity, which is a major transducer of Wnt signaling, is activated in the uterus specifically at the site of implantation in an embryo-dependent manner. These results introduce Wnts as candidate signaling factors that may mediate the communication between the embryo and uterus that initiates implantation.
Wnt/beta-catenin signaling triggers axis formation in Xenopus and zebrafish embryos. I showed that, during embryonic development, beta-catenin-regulated transcriptional activity is first detected in the prospective primitive streak region prior to gastrulation. This demarcates the posterior region of the embryo. This activity then becomes restricted to the elongating primitive streak and to the node. In Xenopus embryos, beta-catenin participates in the formation of the organizer through the activation of the homeodomain transcription factors Siamois and Twin. I obtained evidence that a Siamois/Twin-like binding activity exists in mouse embryos and is localized in the node. These results strongly suggest that, as the case in Xenopus and zebrafish, the Wnt/beta-catenin pathway is involved in establishing embryonic body axes.
Furthermore, using the transgenic mouse line that I generated for these studies, I mapped the transcriptional activity of beta-catenin during mouse embryonic development. These results revealed when and where this activity, and presumably Wnt signaling, is active during the development of several organs and embryonic structures.

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28

Alp, Murat. "A kinetic model of calcium binding to calretinin : experimental measurements and predicted effects on calcium signaling at neuronal synapses /." view abstract or download file of text, 2005. http://wwwlib.umi.com/cr/uoregon/fullcit?p3190505.

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Thesis (Ph. D.)--University of Oregon, 2005.
Typescript. Includes vita and abstract. Includes bibliographical references (leaves 250 - 269). Also available for download via the World Wide Web; free to University of Oregon users.

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29

Wilham, Laura Elizabeth. "The role of [beta]-arrestin in agonist-induced down-regulation of the M₁mAChR." CONNECT TO THIS TITLE ONLINE, 2006. http://etd.lib.umt.edu/theses/available/etd-03022007-104437/.

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30

Ng, Sai-ming. "Characterization of human secretin receptor by the cytosensor microphysiometer system /." Hong Kong : University of Hong Kong, 1998. http://sunzi.lib.hku.hk/hkuto/record.jsp?B19737324.

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31

Wan, Jun. "Elucidation of the JNK pathway mediated by Epstein-Barr virus encoded latent membrane protein 1 /." View abstract or full-text, 2004. http://library.ust.hk/cgi/db/thesis.pl?BICH%202004%20WAN.

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Thesis (Ph. D.)--Hong Kong University of Science and Technology, 2004.
Includes bibliographical references (leaves 105-128). Also available in electronic version. Access restricted to campus users.

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32

Cao, Yang. "Macromolecular crowding effects on the activity of the extracellular signal regulated kinase 2 /." View abstract or full-text, 2008. http://library.ust.hk/cgi/db/thesis.pl?CHEM%202008%20CAO.

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33

Xu, Weiguang. "Solution structure of [Alpha]-syntrophin PH-PDZ tandem domain /." View abstract or full-text, 2005. http://library.ust.hk/cgi/db/thesis.pl?BICH%202005%20XU.

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34

Knapp, Bettina, and Lars Kaderali. "Reconstruction of Cellular Signal Transduction Networks Using Perturbation Assays and Linear Programming." Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2014. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-127239.

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Perturbation experiments for example using RNA interference (RNAi) offer an attractive way to elucidate gene function in a high throughput fashion. The placement of hit genes in their functional context and the inference of underlying networks from such data, however, are challenging tasks. One of the problems in network inference is the exponential number of possible network topologies for a given number of genes. Here, we introduce a novel mathematical approach to address this question. We formulate network inference as a linear optimization problem, which can be solved efficiently even for large-scale systems. We use simulated data to evaluate our approach, and show improved performance in particular on larger networks over state-of-the art methods. We achieve increased sensitivity and specificity, as well as a significant reduction in computing time. Furthermore, we show superior performance on noisy data. We then apply our approach to study the intracellular signaling of human primary nave CD4+ T-cells, as well as ErbB signaling in trastuzumab resistant breast cancer cells. In both cases, our approach recovers known interactions and points to additional relevant processes. In ErbB signaling, our results predict an important role of negative and positive feedback in controlling the cell cycle progression.

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35

Knapp, Bettina, and Lars Kaderali. "Reconstruction of Cellular Signal Transduction Networks Using Perturbation Assays and Linear Programming." Public Library of Science, 2013. https://tud.qucosa.de/id/qucosa%3A27289.

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Perturbation experiments for example using RNA interference (RNAi) offer an attractive way to elucidate gene function in a high throughput fashion. The placement of hit genes in their functional context and the inference of underlying networks from such data, however, are challenging tasks. One of the problems in network inference is the exponential number of possible network topologies for a given number of genes. Here, we introduce a novel mathematical approach to address this question. We formulate network inference as a linear optimization problem, which can be solved efficiently even for large-scale systems. We use simulated data to evaluate our approach, and show improved performance in particular on larger networks over state-of-the art methods. We achieve increased sensitivity and specificity, as well as a significant reduction in computing time. Furthermore, we show superior performance on noisy data. We then apply our approach to study the intracellular signaling of human primary nave CD4+ T-cells, as well as ErbB signaling in trastuzumab resistant breast cancer cells. In both cases, our approach recovers known interactions and points to additional relevant processes. In ErbB signaling, our results predict an important role of negative and positive feedback in controlling the cell cycle progression.

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36

Schwarcz, Leslie Esther. "Linking steroid hormone and Wnt signaling /." view abstract or download file of text, 2006. http://wwwlib.umi.com/cr/uoregon/fullcit?p3211226.

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Thesis (Ph. D.)--University of Oregon, 2006.
Typescript. Includes vita and abstract. Includes bibliographical references (leaves 71-82). Also available for download via the World Wide Web; free to University of Oregon users.

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37

Ng, Sai-ming Samuel, and 吳世明. "Characterization of human secretin receptor by the cytosensor microphysiometer system." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 1998. http://hub.hku.hk/bib/B31219743.

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38

Tai, Chi Shing. "Identification and characterization of Vps74p, a coatomer and SNARE interacting protein involved in membrane traffic /." View abstract or full-text, 2004. http://library.ust.hk/cgi/db/thesis.pl?BIOL%202004%20TAI.

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39

Kawczyński, Wojciech. "Effects of low temperature on nuclear proteins of alfalfa." Thesis, McGill University, 1995. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=23278.

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During the present studies we attempted to answer the following questions: (i) Does low temperature alter the phosphorylation level of proteins in isolated nuclei? (ii) Does the nuclear phosphoprotein population change during a prolonged exposure of seedlings to cold? (iii) Do heat-stable proteins accumulate in the nucleus during a prolonged exposure of seedlings to cold? (iv) Are the answers to the above three questions related to freezing tolerance? A possible relationship between the observed cold-induced changes in phosphoproteins and the level of freezing tolerance was explored by comparing the results of experiments conducted on two cultivars (Apica and Trek) of alfalfa (Medicago sativa L.) which markedly differ in their capacity for cold acclimation.
We show that the phosphorylation level of several nuclear proteins is subject to rapid and reversible enhancement by low temperature. Several phosphoproteins were found to be constitutively present in the nucleus of both cultivars. The cold-induced stimulation of the phosphorylation of many of these proteins was much greater in the relatively freezing tolerant cultivar Apica than in the relatively freezing sensitive cultivar Trek. Population of nuclear phosphoproteins was found to be considerably more complex in Apica than in Trek. During a prolonged exposure of the seedlings to 4$ sp circ$C, additional phosphoproteins were imported into the nucleus of Apica seedlings but not those Trek.
Some heat-stable proteins were constitutively present in the nucleus of both cultivars. However during the 4-day cold treatment, a large accumulation of several additional heat-stable proteins was observed in the tolerant, but not the sensitive, cultivar. (Abstract shortened by UMI.)

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40

Lau, Wai In. "Regulation of STAT3 and PKD through melatonin receptors /." View abstract or full-text, 2009. http://library.ust.hk/cgi/db/thesis.pl?BICH%202009%20LAU.

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41

Zhu, Weidong, and 朱伟东. "APPL1 and APPL2: a pair of adaptor proteins as "yin-and-yang" regulators of insulin signaling in skeletalmuscle." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2011. http://hub.hku.hk/bib/B45980470.

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Summy, Justin Matthew. "Functional domain contributions to signaling specificity between the non-receptor tyrosine kinases c-src and c-yes." Morgantown, W. Va. : [West Virginia University Libraries], 2001. http://etd.wvu.edu/templates/showETD.cfm?recnum=2239.

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Thesis (Ph. D.)--West Virginia University, 2001.
Title from document title page. Document formatted into pages; contains vi, 195 p. : ill. (some col.). Vita. Includes abstract. Includes bibliographical references (p. 182-190).

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Lee, Wai-him, and 李偉謙. "Proteomic analysis of protein phosphorylation in PC12 cells induced bypituitary adenylate cyclase activating polypeptide 38." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2002. http://hub.hku.hk/bib/B30149885.

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Chen, Bin, and 陈斌. "Structural and functional characterization of human APPL2, a novel adaptor protein involved in insulin signaling." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2010. http://hub.hku.hk/bib/B4552757X.

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Cheng, King-yip. "APPL1 as a novel signaling mediator of adiponectin and insulin molecular mechanisms and physiological implications /." Click to view the E-thesis via HKUTO, 2009. http://sunzi.lib.hku.hk/hkuto/record/B42182177.

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Rui, Hongliang. "Regulation of MAPK/JNK signaling pathway and TGF-beta signaling pathway by axin /." View abstract or full-text, 2004. http://library.ust.hk/cgi/db/thesis.pl?BICH%202004%20RUI.

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Thesis (Ph.D.)--Hong Kong University of Science and Technology, 2004.
CD-ROM contains electronic versons of the thesis in pdf and word format. Includes bibliographical references (leaves 129-151). Also available in electronic version. Access restricted to campus users.

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Yip, Rupert G. "Signal Transduction Mechanisms for the Stimulation of Lipolysis by Growth Hormone: A Dissertation." eScholarship@UMMS, 1994. https://escholarship.umassmed.edu/gsbs_diss/108.

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The purpose of this study was to investigate the mechanism of action of lipolysis by growth hormone in rat adipocytes. GH-induced lipolysis, in contrast to that of isoproterenol (ISO), is slow in onset (lag time >1h), small in magnitude (~2X basal). and requires corticosteroid. Evidence for direct coupling between GH receptors and adenylyl cyclase or G-proteins is lacking, and although we could detect no measurable change in cAMP content after treatment with GH + dexamethasone (Dex), it is likely that cAMP activation of protein kinase A is a central event in GH-induced lipolysis. Rp-cAMPS, a competitive antagonist of cAMP was equally effective in decreasing lipolysis in tissues treated with GH/Dex or a comparably lipolytic dose of ISO. Incorporation of 32P from γ-32P-ATP into kemptide, a synthetic oligopeptide substrate for protein kinase A, was increased in homogenates of GH/Dex-treated tissue. This increase was correlated with increased lipolysis. Earlier estimates based upon 32P-ribosylation of Gi catalysed by pertussis toxin (PTx) suggested that the abundance of Gi in adipocyte membranes was decreased 4h after treatment of hypophysectomized rats with GH. We therefore examined the possibility that changes in amount or distribution of G-proteins in adipocyte membranes might account for the lipolytic action of GH. Homogenates of GH/Dex-treated and control adipocytes were subjected to differential centrifugation and the abundance of G-proteins in low speed, l6k x g (16k), pellets and high speed, 100k x g (100k), pellets were determined by quantitative Western analysis with densitometry. A 35% loss of Giα2 from the l6k pellet compared from tissues treated with GH/Dex was associated with a 70% increase of Giα2 in the 100k pellet. No change in Gsα was observed in the l6k pellet but a 35% loss of Gsα was seen in the 100k pellet. The G proteins in the l6k pellet were fractionated on a continuous sucrose gradient followed by quantitation with Western analysis or autoradiography after 32P-NAD ribosylation. Giα2 was consistently shifted from heavier to lighter fractions of the l6k pellet after treatment with GH/Dex. Similar shifts of Gsα were not seen. The distribution of 32P-labelled proteins was comparably altered after incubation of homogenates of control and GH/Dex treated adipocytes with PTx and 32P-NAD. These shifts were blocked by treatment of adipocytes with 100μM colchicine which also blocked the lipolytic action of GH/Dex. We propose that an action of GH/Dex on the cytoskeleton of fat cells may change the cellular distribution of G-proteins in a manner that produces a relative decrease in the tonic inhibitory influence of Gi on adenylyl cyclase.

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Lee, Chi-wai, and 李志慧. "The characterization of the LKB1-AMPK pathway in hepatocellular carcinoma." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2010. http://hub.hku.hk/bib/B45984190.

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Salomon, Steven. "Expression of the formin Daam 1 in pyramidal neurons of the hippocampus affects spine morphology." Thesis, McGill University, 2006. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=98789.

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Formins, also known as formin homology (FH) proteins, are involved in a wide range of actin-mediated processes. The Diaphanous-related formin Daam1 (Dishevelled-associated activator of morphogenesis) interacts with the PDZ domain protein Dishevelled, and is required to establish planar cell polarity in Xenopus. Through a yeast two-hybrid screen, I characterized a PDZ-mediated interaction between the C-terminus of Daam1 and the PDZ domains 456 of GRIP1. In dissociated rat hippocampal cultures, Daam1 expression was seen throughout the soma and dendrites in a punctate pattern. Furthermore, co-staining with a synaptic marker suggests that Daam1 could be associated with post-synaptic specializations. Dendritic spines are enriched with actin filaments, and based on the subcellular localization of Daam1 and the evidence that formins are involved in regulating actin polymerization, I hypothesized that Daam1 might play a role in dendritic spine morphology. In order to investigate the functional roles for Daam1, viral vectors were developed using the Semliki-Forest defective viral vector to over-express the full-length Daam1 protein and a Daam1 lacking the PDZ-binding motif. The over-expression of the full-length Daam1 in organotypic hippocampal slices showed a punctate distribution throughout the dendritic shaft, with the occasional appearance in spines, resulting in an overall increase in dendritic spine length. This suggests that formins, such as Daam1, could potentially regulate spine morphology.

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D'Rozario, Robert S. G. "Conformational dynamics of proline-containing transmembrane helices." Thesis, University of Oxford, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.670181.

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