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Dissertations / Theses on the topic 'Proteins. Ion exchange. Ions'

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1

Pan, L. C. "Ion exchange of proteins." Thesis, Swansea University, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.638406.

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The aims of the present work were to assess the nature and importance of the intermolecular and surface interactions in ion exchange processes at different conditions and also to develop a mathematical model for the prediction of equilibrium constants. The equilibrium uptake of two proteins (bovine serum albumin, myoglobin) on hydrophilic ion exchangers was studied at different pH values and ironic strengths. Equilibrium isotherm analysis allowed the determination of the thermodynamic equilibrium constants (K) for each condition. The affinity of the hydrophilic cellulosic ion exchanger (Whatma
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2

Avkiran, Metin. "Pathophysiological roles, pharmacological inhibition and cellular regulation of the cardiac sarcolemmal sodium/hydrogen exchanger." Thesis, University of Bath, 2002. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.601638.

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3

Cao, Liming. "Protein Separation with Ion-exchange Membrane Chromatography." Link to electronic thesis, 2005. http://www.wpi.edu/Pubs/ETD/Available/etd-050405-174109/.

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4

Ahmed, Manzoor. "Modelling the ion exchange equilibria of proteins." Thesis, University of Reading, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.242335.

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5

Goodall, Sharon. "Selective separation of whey proteins using ion exchange membranes." Thesis, University of Reading, 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.499369.

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Due to increasing use of whey proteins in functional foods and pharmaceutical products there is a need to develop processes to selectively separate and concentrate individual whey proteins. Existing methods for large-scale separation of whey proteins have included reverse osmosis, ultrafiltration, microfiltration and ion exchange. This study looked specifically at the use of synthetic microporous membranes, with functional groups covalently attached, to selectively separate β-lactoglobulm bovine serum albumin (BSA), α-lactalbumin, lactoferrin, lactoperoxidase and finally caseinomacropeptides f
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6

Kumar, Guneet. "Design of anion exchange cellulose hydrogel for large proteins." Diss., This resource online, 1994. http://scholar.lib.vt.edu/theses/available/etd-06062008-163945/.

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7

Langford, John F. Jr. "Effects of adsorbent structure and adsorption on transport phenomena in ion-exchange chromatography." Access to citation, abstract and download form provided by ProQuest Information and Learning Company; downloadable PDF file, 250 p, 2007. http://proquest.umi.com/pqdweb?did=1251904681&sid=1&Fmt=2&clientId=8331&RQT=309&VName=PQD.

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8

Trilisky, Egor. "Stationary and mobile phase selection for ion-exchange chromatography of viruses." Access to citation, abstract and download form provided by ProQuest Information and Learning Company; downloadable PDF file, 266 p, 2009. http://proquest.umi.com/pqdweb?did=1674096451&sid=1&Fmt=2&clientId=8331&RQT=309&VName=PQD.

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9

Borghi, Andrea. "Cellulose hollow fibers membranes for ion-exchange chromatography." Master's thesis, Alma Mater Studiorum - Università di Bologna, 2020. http://amslaurea.unibo.it/20289/.

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The development of an efficient and cost-effective purification process is the objective of protein separation. Usually, the most common procedure is chromatography, typically performed in a packed bed column; however, the cost is relatively high. Nowadays, one of the most interesting and promising method is the employment of particular membranes, the hollow fibers, in the standard chromatographic operations. Hollow fibers membranes were developed in the 1960s, specifically for reverse osmosis; nonetheless, they are now largely applied in both liquid and gaseous applications. In this proje
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10

Bossuyt, Julie. "Sodium-calcium exchange and caveolins." MU has:, 2002. http://wwwlib.umi.com/cr/mo/fulltext?p3052149.

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11

Chen, Xin. "Development of Monolithic Stationary phases for Cation-Exchange Capillary Liquid Chromatography of Peptides and Proteins." BYU ScholarsArchive, 2011. https://scholarsarchive.byu.edu/etd/2467.

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This dissertation focuses on the preparation of polymeric monolithic capillaries for ion exchange chromatography of peptides and proteins, since polymeric monoliths have shown promise for providing improved protein separations. Characteristics of monolithic columns include low back pressure, simplicity of fabrication and biocompatibility. Preparation of strong and weak cation-exchange monolithic stationary phases in 75 μm I.D. capillaries by direct in situ copolymerization was achieved using various functional monomers including sulfopropyl methacrylate, phosphoric acid 2-hydroxyethyl methacry
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12

Castiblanco, Adriana P. "Expression and Purification of Engineered Calcium Binding Proteins." Digital Archive @ GSU, 2009. http://digitalarchive.gsu.edu/chemistry_theses/20.

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Previous studies in Dr. Yang’s laboratory have established a grafting, design, and subdomain approach in order to investigate the properties behind Ca2+-binding sites located in Ca2+-binding proteins by employing engineered proteins. These approaches have not only enabled us to isolate Ca2+-binding sites and obtain their Ca2+-binding affinities, but also to investigate conformational changes and cooperativity effects upon Ca2+ binding. The focus of my thesis pertains to optimizing the expression and purification of engineered proteins with tailored functions. Proteins were expressed in E. col
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13

Liang, Juan [Verfasser]. "Theoretical and experimental study of single and binary components adsorption of proteins onto ion-exchange chromatographic media / Juan Liang." München : Verlag Dr. Hut, 2014. http://d-nb.info/1053859538/34.

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14

Alves, Thaís Barboni. "Prospecção de proteínas bioativas secretadas por fungos filamentosos do gênero Aspergillus /." Araraquara, 2017. http://hdl.handle.net/11449/151625.

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Orientador: Luis Henrique Souza Guimarães<br>Banca: Ariela Veloso de Paula<br>Banca: Rosimeire Cristina Linhari Rodrigues Pietro<br>Banca: Hamilton Cabral<br>Banca: Sandra Regina Pombeiro Sponchiado<br>Resumo: Produtos naturais bioativos podem ser produzidos por organismos de todos os reinos, de procariotos a eucariotos. Entre esses, os fungos filamentosos são fontes abundantes de diversos metabólitos, como proteínas e enzimas, as quais vêm se destacando devido as suas diversas atividades biológicas, com aplicações não só na área farmacêutica e médica como também, nas indústrias alimentícia, d
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15

Voswinkel, Linda Elisabeth [Verfasser], Ulrich [Akademischer Betreuer] [Gutachter] Kulozik, and Matthias [Gutachter] Kraume. "Fractionation of major and minor Whey Proteins using Membrane-based Ion-exchange Chromatography / Linda Elisabeth Voswinkel ; Gutachter: Ulrich Kulozik, Matthias Kraume ; Betreuer: Ulrich Kulozik." München : Universitätsbibliothek der TU München, 2016. http://d-nb.info/1123729204/34.

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16

Rostaing, Christine. "Etude chromatographique de la keratine." Paris 6, 1988. http://www.theses.fr/1988PA066514.

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Utilisation de la chromatographie par front de permutation pour etudier le comportement du cheveu. Les interactions electrostatiques, hydrophobes, et par complexation, ont ainsi ete quantifiees en fonction du ph
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17

OLIVEIRA, JOAO E. de. "Purificacao de hormonio de crescimento humano recombinante obtido no espaco periplasmico de ESCHERICHIA COLI, visando sua aplicacao clinica." reponame:Repositório Institucional do IPEN, 1999. http://repositorio.ipen.br:8080/xmlui/handle/123456789/10755.

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18

Brand, Janina [Verfasser]. "Modification of the Structural Properties of Egg White by Mechanical Methods prior to Membrane Based Ion Exchange Chromatographic Fractionation of Egg White Proteins / Janina Brand." München : Verlag Dr. Hut, 2017. http://d-nb.info/1126298212/34.

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19

Hedhammar, My. "Strategies for facilitated production of recombinant proteins in escherichia coli." Doctoral thesis, KTH, School of Biotechnology (BIO), 2005. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-471.

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<p>The successful genomic era has resulted in a great demand for efficient production and purification of proteins. The main objective of the work described in this thesis was to develop methods to facilitate recovery of target proteins after recombinant production in Escherichia coli.</p><p>A positively charged purification tag, Z<sub>basic</sub>, has previously been constructed by protein design of a compact three-helix bundle domain, Z. The charged domain was investigated for general use as a fusion partner. All target proteins investigated could be selectively captured by ion-exchange chro
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20

Marobhe, Nancy. "Water Supply in Tanzania and Performance of Local Plant Materials in Purification of Turbid Water." Doctoral thesis, KTH, Mark- och vattenteknik, 2008. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-4781.

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Water supply services in urban and rural areas of Tanzania were reviewed and specific studies were carried out on water supply and on purification of turbid water sources using locally available plant materials in rural villages of Singida Rural District. The review showed that large proportions of urban and rural populations in Tanzania face acute water supply problems mainly due to poor planning, implementation and management of water supply projects, including an inability to address social, technical, operation and maintenance and financial issues. Laboratory-scale experiments studied the
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21

Kruger, Sarah Jane, and n/a. "Characterisation of Proteins from Grevillea robusta and NMR Studies of the Serine Protease Inhibitor." Griffith University. School of Science, 2004. http://www4.gu.edu.au:8080/adt-root/public/adt-QGU20040618.150708.

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Proteins that recognise the sugar surface structures on cells have an enormous potential to be used as tools in the characterisation of these structures. A group of proteins, called lectins, have been identified that can bind to carbohydrate complexes on the receptors of cells. The crude extract from Grevillea robusta seeds was found to contain lectin-like proteins that were different from most other lectins, as they would specifically target the receptors of white blood cells and not those found on red blood cells. Therefore, the lectin isolated from G.robusta could be used as a tool to id
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22

Addo, Martin Adomako. "Developments in ion exchange media for protein separation." Thesis, University of Bath, 1988. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.760580.

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23

Gosling, I. S. "Characterisation of ion exchangers for protein recovery." Thesis, Swansea University, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.637080.

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24

Sloupová, Klára. "Izolace čistých aminokyselin z pšeničných otrub." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2021. http://www.nusl.cz/ntk/nusl-449764.

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Wheat bran is a promising material containing a wide range of useful components, including proteins. In addition, it is produced in significant volumes. Currently, wheat bran is used for the production of energy by combustion and for feed purposes. Gradually, new methods of valorization of this material are being sought. One of the possibilities of using wheat bran is the isolation of proteins, hydrolysis, and separation of selected amino acids. This diploma thesis deals with this issue, it is focused on the recovery of arginine and leucine from a protein isolate. Proteins were extracted from
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25

Pathange, Lakshmi Prasad. "Characterization of protein microstructure by various chromatographic techniques." Diss., Virginia Tech, 2007. http://hdl.handle.net/10919/26851.

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Due to the rising health care costs and with the advent of biogenerics, there is a growing demand to develop new and reliable techniques to characterize proteins and biopharmaceuticals. In addition, characterization aids in understanding the intricate relationship between a protein's structure and its function. To address this challenge, two protein structural parameters, 1) amino acid surface area and 2) amino acid microstructure, were chosen to be investigated. Two chromatographic techniques, 1) ion exchange chromatography (IEC) and 2) immobilized metal affinity chromatography (IMAC), were u
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26

Rejaldien, Moegamat Zain. "Base metal equilibrium prediction on ion exchange resin." Thesis, Cape Technikon, 2002. http://hdl.handle.net/20.500.11838/875.

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Thesis (MTech (Chemical Engineering)--Cape Technikon, Cape Town, 2002<br>This study is the first phase of an investigation into an alternative process to recover base metals from solution, namely ion exchange. An identified ion exchange resin was employed to study the recovery of copper, nickel and cobalt from electrolytic solutions. The main focus of the investigation was the prediction of equilibrium conditions in this multi-component system using a batch configuration. In order to predict equilibrium conditions. existing multi-component isotherms such as Fritz and Schleunder were test
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27

EL-CORAB, MARIANA D. M. K. "Caracterização da crotamina e seu efeito sobre a contratilidade da musculatura lisa do ducto deferente de rato." reponame:Repositório Institucional do IPEN, 2015. http://repositorio.ipen.br:8080/xmlui/handle/123456789/28041.

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Submitted by Pedro Silva Filho (pfsilva@ipen.br) on 2017-11-22T17:22:11Z No. of bitstreams: 0<br>Made available in DSpace on 2017-11-22T17:22:11Z (GMT). No. of bitstreams: 0<br>A crotamina, um peptídeo catiônico que possui 42 aminoácidos e 4,88 kDa, é proveniente do veneno de Crotalus durissus terrificus. Ela apresenta características que permitem sua forte interação com alvos moleculares e membranas biológicas e assim foi o primeiro peptídeo de veneno a ser classificado como um CPP (cell penetrating peptide), justificando seus importantes efeitos biológicos e suas diversas atividades farmaco
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28

Ma, Yiu Wa. "Fixed bed removal of heavy metal ions by chelating ion exchange." Thesis, Queen's University Belfast, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.491880.

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Column runs with varied operation conditions of flowrate, feed concentration and particle size have been conducted to observe the sorption of nickel, copper and zinc by the chelating resin. They all show a typical constant-pattern, steep curve in the first part of the breakthrough curve and the slope would dramatically reduce at a breakpoint such that it would take extremely long time to reach 100% breakthrough level. ~ The Modified Bed Depth Service Time (BDST) model incorporated with a timedependent bed capacity has been used together with the Empty Bed Residence Time (EBRT) model to analyze
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29

Sines, Brian James. "Evaluation of Phenomena that Determine the Performance of Immunoaffinity, Peptide-Based and Ion Exchange Affinity Sorbents." Diss., Virginia Tech, 2000. http://hdl.handle.net/10919/29841.

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Insufficient supply and pathogen safety concerns regarding plasma-derived therapeutic proteins, such as fibrinogen and immunoglobulins, have been the impetus for the development of genetic engineering techniques and separations methods for the economical and safe production of these proteins. This study is concerned with the isolation of these important therapeutics from complex media. Immunoaffinity chromatography has been an important method in the isolation of these products, typically being implemented as the final cleanup step yielding an extremely pure, homogenous final product. Howev
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30

Ming, Fang. "Manufacture, characterisation, modelling and application of a novel ion-exchanger for protein separation." Thesis, University of Bath, 1991. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.294108.

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31

Lan, Qingdao. "Development of a continuous ion exchange system for protein recovery using liquid-solid circulating fluidized bed." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2001. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp04/NQ58401.pdf.

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32

Dean, Jill Barbara. "Development of a novel material for the selective removal of metal ions from dilute solutions." Thesis, University of York, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.336570.

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33

Koumanov, Assen. "Theoretical prediction of ionisation properties of proteins /." Stockholm, 2003. http://diss.kib.ki.se/2003/91-7349-535-2.

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34

PIRES, MARIA A. F. "Aplicacao da cromatografia de ions no controle de materiais de interesse nuclear." reponame:Repositório Institucional do IPEN, 1989. http://repositorio.ipen.br:8080/xmlui/handle/123456789/9918.

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35

Ndayambaje, Guillaume. "Sorption properties of natural zeolites for the removal of ammonium and chromium ions in aqueous solution." University of the Western Cape, 2011. http://hdl.handle.net/11394/5425.

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>Magister Scientiae - MSc<br>There are huge amount of natural clinoptilolite available in South Africa which can be utilised for wastewater treatment of ammonia and chromium if their characteristics are properly known. However, these deposits have not been well characterised but in this study, the untreated clinoptilolite materials were fully characterised using techniques such as SEM-EDS, HRTEM-SAED, XRD, XRF, FTIR and BET. After acid pretreatment with several extractions, the pretreated samples were again characterised using the above mentioned techniques. These pretreated materials were us
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36

Pedersen, Anders Hjordt. "Molecule-based magnetic materials of the ReIV ion." Thesis, University of Edinburgh, 2017. http://hdl.handle.net/1842/28885.

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The [ReCl6]2-, [ReBr6]2- and [ReCl4(ox)]2- anions are crystallised with the organic 4,4’- bipyridinium dication (4,4-H2bipy). Magnetometry reveals exotic behaviour of the [4,4’- H2bipy][ReCl6] and [4,4’-H2bipy][ReBr6] salts which demonstrate spin-canting, antiferromagnetic exchange interactions and metamagnetism. Single crystal X-ray structures at T = 3, 14 and 20 K of the [4,4’-H2bipy][ReBr6] salt reveal the behaviour to be purely of magnetic origin as no structural changes are observed. For the [4,4’-H2bipy][ReCl4(ox)] compound an antiferromagnetic exchange interaction of 10.2 cm-1 between t
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37

MANOSSO, HELENA C. "Desenvolvimento de eletrodos de troca iônica eletroquímica para o tratamento de rejeitos contendo íons crômio ou césio." reponame:Repositório Institucional do IPEN, 2006. http://repositorio.ipen.br:8080/xmlui/handle/123456789/11439.

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38

El-Masri, Nasrein. "Ion-association complexes of thallium and mercury with rhodamine 6G in aqueous solution." Scholarly Commons, 1988. https://scholarlycommons.pacific.edu/uop_etds/2170.

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39

Gucinski, Ashley Christine. "Gas Phase Structural Studies of Peptide Fragment Ions: Structural Insights into Mass Spectrometry Fragmentation Mechanisms." Diss., The University of Arizona, 2011. http://hdl.handle.net/10150/202766.

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This dissertation presents extensive structural studies of gas-phase peptide fragment ions, with a specific focus on b₂⁺ ions. Fragment ion structures can provide important insights into peptide fragmentation mechanisms. Based on the structures formed, information about the preference of competing b ion formation pathways can be obtained. b₂⁺ ion structures are of interest because of their large relative abundances in MS/MS spectra, which are difficult to predict. Prior to this work, only a few b₂⁺ ion structures were determined; these systems featured only aliphatic residues and all formed ox
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MARQUES, MARIA N. "Cromatografia de ions aplicada na especia‡ao de cromio hexavalente em amostras de interesse ambiental." reponame:Repositório Institucional do IPEN, 1999. http://repositorio.ipen.br:8080/xmlui/handle/123456789/10739.

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41

Chang, Chih-Hsiang. "Proteomic studies on protein N-terminus and peptide ion mobility by nano-scale liquid chromatography/tandem mass spectrometry." Doctoral thesis, Kyoto University, 2021. http://hdl.handle.net/2433/263596.

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42

Dietrich, Theo Henry. "The removal of heavy metals from dilute aqueous streams by the use of ion exchange resins." Thesis, Cape Technikon, 1998. http://hdl.handle.net/20.500.11838/887.

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Thesis (MTech (Chemical Engineering)--Cape Technikon, Cape Town, 1998<br>Ion exchange resins are widely used to remove or concentrate heavy metals from aqueous solutions or slurries.This thesis attempts to properly evaluate the interaction between ion exchange resins and heavy metals at trace metal concentrations.The durability of the resins and their effectiveness in real slurries were also investigated. In this study, a chelating resin, as well as a cation, and anion exchange resin was contacted with aqueous solutions of heavy metals in both free and complexed form. Zinc, nickel and c
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43

Allen, Frances Isabel. "Electron capture by highly charged ions from surfaces and gases." Doctoral thesis, Humboldt-Universität zu Berlin, Mathematisch-Naturwissenschaftliche Fakultät I, 2008. http://dx.doi.org/10.18452/15720.

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In dieser Arbeit werden hochgeladene, mit einer Electron Beam Ion Trap produzierte Ionen für die Erforschung des Elektroneneinfangs von Oberflächen und Gasen eingesetzt. Die Untersuchungen mit Gastargets konzentrieren sich auf die Energieabhängigkeit der Verteilung der K-Schalen-Röntgenstrahlen, die nach Elektroneneinfang in Rydberg-Zustände von Ar-17+ und Ar-18+ Ionen am Ende einer Kaskade von Elektronenübergängen entstehen. Die Ionen werden von der Ionenquelle mit einer Energie von 2 keV/u extrahiert, ladungsselektiert und anschließend bis auf 5 eV/u abgebremst, um dann mit einem
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44

Nicolas-Simonnot, Marie-Odile. "Contribution à l'étude de la chromatographie frontale des protéines par échange d'anions : Application à l'albumine du serum bovin bovin." Vandoeuvre-les-Nancy, INPL, 1991. http://docnum.univ-lorraine.fr/public/INPL_T_1991_NICOLAS_SIMONNOT_M_O.pdf.

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La chromatographie d'échange d'anions mettant en jeu une protéine fait intervenir simultanément plusieurs phénomènes : l'échange d'ions proprement dit, ainsi que d'autres réactions, essentiellement de dissociation, en phase liquide ou solide. La démarche adoptée ici consiste à découpler toutes les contributions et à les examiner séparément, avant de les regrouper dans un modèle global. On étudie le comportement des supports échangeurs d'ions faibles en fonction de l'environnement ionique, les interactions entre les électrolytes faibles (tampons de pH) et les échangeurs d'anions, les interactio
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45

Mustafa, Morad. "Ion Permeation through Membrane Channels: Molecular Dynamics Simulations Studies." Diss., CLICK HERE for online access, 2008. http://contentdm.lib.byu.edu/ETD/image/etd2477.pdf.

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Lopes, João Henrique 1982. "Modificação da superficie de vidros bioativos com ions cálcio e tratamento térmico." [s.n.], 2010. http://repositorio.unicamp.br/jspui/handle/REPOSIP/250000.

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Orientador: Celso Aparecido Bertran, Italo Odone Mazali<br>Dissertação (mestrado) - Universidade Estadual de Campinas, Instituto de Quimica<br>Made available in DSpace on 2018-08-15T20:33:27Z (GMT). No. of bitstreams: 1 Lopes_JoaoHenrique_M.pdf: 3741277 bytes, checksum: 839c748f4fd68d1140366429af1efbc5 (MD5) Previous issue date: 2010<br>Mestrado<br>Físico-Química<br>Mestre em Química
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47

Dias, Jailson Cardoso. "Determinação de acetato, nitrato, ferro(II), ferro(III) e cobre em etanol combustivel por cromatografia de ions." [s.n.], 2010. http://repositorio.unicamp.br/jspui/handle/REPOSIP/248380.

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Orientador: Lauro Tatsuo Kubota<br>Tese (doutorado) - Universidade Estadual de Campinas, Instituto de Quimica<br>Made available in DSpace on 2018-08-15T10:20:51Z (GMT). No. of bitstreams: 1 Dias_JailsonCardoso_D.pdf: 1120921 bytes, checksum: 080b276b57f19a203109def137925235 (MD5) Previous issue date: 2010<br>Resumo: O contexto geral deste trabalho se insere na área de desenvolvimento de métodos analíticos para a separação cromatográfica e detecção de íons em biocombustíveis. Diversos procedimentos de otimização (uni e multivariada) foram executados inicialmente visando estabelecer as melhore
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48

Claypool, Ian Randolph. "A theoretical and numerical study of the use of grid embedded axial magnetic fields to reduce charge exchange ion induced grid erosion in electrostatic ion thrusters." Columbus, Ohio : Ohio State University, 2007. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=osu1172690635.

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Pierini, Giulia. "Preparation and characterization of grafted nonwoven membranes for bioseparations." Master's thesis, Alma Mater Studiorum - Università di Bologna, 2016. http://amslaurea.unibo.it/11903/.

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Protein purification plays a crucial role in biotechnology and biomanufacturing, where downstream unit operations account for 40%-80% of the overall costs. To overcome this issue, companies strive to simplify the separation process by reducing the number of steps and replacing expensive separation devices. In this context, commercially available polybutylene terephthalate (PBT) melt-blown nonwoven membranes have been developed as a novel disposable membrane chromatography support. The PBT nonwoven membrane is able to capture products and reduce contaminants by ion exchange chromatography. The
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Ghebremichael, Kebreab Afwerki. "Moringa seed and pumice as alternative natural materials for drinking water treatment." Doctoral thesis, KTH, Land and Water Resources Engineering, 2004. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-85.

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<p>Pumice and the Moringa oleifera (MO) seed were investigated as alternative natural materials for drinking water treatment based on problems identified at the Stretta Vaudetto water treatment plant in Eritrea. </p><p>Lab and pilot scale studies showed that pumice was a suitable alternative material for dual media filtration. Conversion of the sand filters at Stretta Vaudetto to pumice-sand media would significantly improve performance of the filtration units. The coagulant protein from the MO seed was purified in a single-step ion exchange purification method. The parameters for batch purifi
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