Dissertations / Theses on the topic 'Protest action'
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Cable, Jonathan. "Protest in action : an examination of the production, media representation and reflexivity of protest group communications strategies and protest tactics." Thesis, Cardiff University, 2012. http://orca.cf.ac.uk/33637/.
Full textAnderson, Jonathan Mark. "Environmental direct action : making space for new forms of political community?" Thesis, University of Bristol, 2000. http://hdl.handle.net/1983/470c8929-f448-4d1f-876b-78bdbad5f40c.
Full textLandman, Todd. "Agents of change : the comparative impact of social movements." Thesis, University of Essex, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.310084.
Full textPurkis, Jonathan. "A sociology of environmental protest : Earth First and the theory and practice of anarchism." Thesis, Manchester Metropolitan University, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.341396.
Full textRoseneil, Sasha. "Feminist political action : the case of the Greenham Common Women's Peace Camp." Thesis, London School of Economics and Political Science (University of London), 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.283148.
Full textMcKahan, Jason Grant. "Hollywood counterterrorism: violence, protest and the Middle East in U.S. action feature films /." Tallahassee, Florida : Florida State University, 2009. http://etd.lib.fsu.edu/theses/available/etd-11162009-124125/.
Full textAdvisor: Andrew Opel, Florida State University, College of Communication and Information, School of Communication. Title and description from dissertation home page (viewed on May 26, 2010). Document formatted into pages; contains x, 370 pages. Includes bibliographical references.
Daly, Belinda Merle Joanne. "Direct action environmental protest in Britain : a critique of radical environmentalism and environmrntal ethics." Thesis, Imperial College London, 2005. http://hdl.handle.net/10044/1/11216.
Full textÅkerström, Linda. "Ny Protest? : En fallstudie av rörelsen Planka.nu." Thesis, Södertörn University College, School of Social Sciences, 2006. http://urn.kb.se/resolve?urn=urn:nbn:se:sh:diva-470.
Full textThe aim of this paper is to analyse the social movement Planka.nu with reference to the theory of new social movements in order to engage in a discussion about the presence of “old” tradition and “new” trends. As well as empirical, the purpose is also theoretical and methodological: to examine, and extend, the discussion about new and old social movements. A qualitative method is used. The analytical framework is based on a combination of theories of social movements and new social movements. Planka.nu was started in 2003 in Stockholm. The movement uses the Internet to organise free-riding on the public buses and trains with the aim of pressuring regional politicians into fully financing public transportation by progressive taxation. In all aspects discussed, both old and new characteristics were found. Behind a rhetoric that to a large extent resembles that of the traditional Swedish labour movement lies a redefinition of values, ideas and strategies that correspond with the theory of new social movements. By adopting a theoretical outlook on social movements that questions the existence of two divided blocs, a more nuanced discussion of the combination of old and new aspects could be held.
Di, Méo Marion. "Une démocratie à l’épreuve des mouvements sociaux : le cas du Chili post-dictatorial de 1988 à nos jours." Thesis, Aix-Marseille, 2018. http://www.theses.fr/2018AIXM0656.
Full textThis thesis aims to give an account of the evolution, in Chile, of the management of the protest events by the institutions in charge of public order since the return to democracy in 1990. It aims to examine the way in which a country once ruled by repression frames, once the democracy returned, the protests of different groups of society. The thesis also questions the existence of a differentiated treatment of the protest events according to the public mobilized. The investigation was conducted between March 2015 and May 2017 and is based on a material consisting of observations, interviews, press archives. This work is composed of three parts. The first examines the elements that have shaped the Chilean political context of the transition, which is largely unfavorable to collective action. It also analyzes the characteristics and the institutional culture of the police in charge of policing protest, by observing how are articulated the military character of this institution and the bases of the doctrine of protest policing. In the second, the mobilizations of the indigenous peoples and students of Chile are lengthily deciphered, in particular the question of their repertoire of action and the interactions between these groups, the Chilean State and the police forces. The third part is devoted to the way in which the recent past of Chile becomes the issue of speeches and mobilizations, and is closely interested in different days of commemoration. Finally, it examines the effects of police militarization on law enforcement, and on the representations of the world surrounding its professional practices
Asterlund, Kent. "The Gezi Protest : A study of different processes behind the mass mobilization." Thesis, Linnéuniversitetet, Institutionen för samhällsstudier (SS), 2014. http://urn.kb.se/resolve?urn=urn:nbn:se:lnu:diva-35231.
Full textWestin, Gustaf. "Voter Elasticity and Political Protest : A quantitative analysis in an American context." Thesis, Uppsala universitet, Statsvetenskapliga institutionen, 2021. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-431226.
Full textSebastian, Eugene Francis. "PROTEST FROM THE FRINGE: Overseas Students and their Influence on Australia’s Export of Education Services Policy 1983-1996." Discipline of Government and International Relations, 2010. http://hdl.handle.net/2123/5833.
Full textThe thesis investigates the motivations behind, the methods used in, and the results of the overseas students’ collective action contesting the measures, which the Australian government introduced from 1983 to 1996. As a group of temporary residents located outside the boundaries of domestic political systems, yet within the core of Australia’s revenue earnings, overseas students independently mobilised in an attempt to influence the Australian Government policy on education from a position of limited political, social and legal rights. As temporary residents on short-term permits fully regulated under prescribed immigration rules, overseas students employed conventional repertoires of contention— they established formal structures, adopted action tools, framed their claims, internationalised their protest, formed alliances — in an attempt to mobilise resources and access existing avenues to influence government’s export of education services policy. Their mobilisation response and campaign strategy achieved modest success in securing some policy concessions, particularly during the early stages of education aid reform. Their strategy, however had to evolve as the fledgling export of education services expanded and eventually they shifted their position to fully embrace and reinterpret the government’s own ‘language of liberalisation’, which they used to greater effectiveness in making subsequent claims. Overseas students ability to procure concessions is derived not from their political or universal rights to education, but from their ability to influence policy changes based on their importance and strategic location in the Australian economy. In other words, government, universities and industry stakeholders have increasingly become dependent on substantial revenue earnings derived from overseas students and have become susceptible to potential chaos that may be precipitated if current students withdrew from the economy, or potential students choosing alternative education service destinations.
Holm, Tanya. "Agens : om konsten att se handling bortom det förväntade." Thesis, Södertörn University College, School of Social Sciences, 2005. http://urn.kb.se/resolve?urn=urn:nbn:se:sh:diva-326.
Full textThe law which makes the purchase, or the attempt to purchase, temporary sexual services a criminal offence has been implemented in Sweden for more than six years. It appears as if very few, possibly no, sex-selling women have used the law to report men who have bought their services during these years. How can this be understood? With this thesis I suggest a new way of thinking on agency. A way which questions the traditional view on action whereby agency equals to act in certain, predefined ways. My theoretical perspective proceeds from the idea that action manifests not only in forms we usually discern but also in shapes we tend to overlook, that agency as a phenomena exists independently of beholders’ abilities to see and recognize actions for what they mean to the performer. By examining what the act to not report sex-buyers means for four women I find that women express their dislike of the law as such as well as of the dogmas it’s based upon by not using it. The women also find reporting incompatible with their own interests as they define them. Therefore I conclude that their repudiation from the law is a political practice, a protest against a law they find unjust and a possibility, for those who wish, to see their opposition against Swedish prostitution policy.
Rawlins, L. Shelley. "Collective Protesting as Existential Communication: A Phenomenology of Risk, Responsibility, and Ethical Attendance." OpenSIUC, 2020. https://opensiuc.lib.siu.edu/dissertations/1791.
Full textNiyazbekov, Nurseit. "Protest mobilisation and democratisation in Kazakhstan (1992-2009)." Thesis, University of Oxford, 2013. http://ora.ox.ac.uk/objects/uuid:494a3742-e7d6-4adf-8728-e644a3f7f249.
Full textDoolen, Joseph. "Protest Movements and the Climate Emergency Declarations of 2019: A New Social Media Logic to Connect and Participate in Politics." Thesis, Uppsala universitet, Institutionen för informatik och media, 2020. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-421114.
Full textManukyan, Alla. "Fraudulent Elections, Political Protests, and Regime Transitions." Digital Archive @ GSU, 2011. http://digitalarchive.gsu.edu/political_science_diss/21.
Full textLukyanova, Yulia. "Manufacturing dissent in Russia : a discursive psychological analysis of protesters' talk." Thesis, University of Edinburgh, 2017. http://hdl.handle.net/1842/23578.
Full textHoffmann, Matthias Christoph. "Exploring the Facebook Networks of German Anti-Immigration Groups." Doctoral thesis, Università degli studi di Trento, 2020. http://hdl.handle.net/11572/254712.
Full textWilkins, Denise Joy. "Power to the Tweeple? : the role of social media in the bridging and setting of boundaries in collective action." Thesis, University of Exeter, 2018. http://hdl.handle.net/10871/33706.
Full textZabic, Sarah D. "Praxis, Student Protest, and Purposive Social Action: The Humanist Marxist Critique of the League of Communists of Yugoslavia, 1964-1975." Kent State University / OhioLINK, 2010. http://rave.ohiolink.edu/etdc/view?acc_num=kent1279565524.
Full textKoen, Devon William. "Investigating the potential of social media in instigating protest action : comparative study between Occupy Wall Street and Occupy JSE events." Thesis, Nelson Mandela Metropolitan University, 2014. http://hdl.handle.net/10948/d1021071.
Full textDaku-Mante, Jacqueline G. "An analysis of civil disobedience with specific reference to the role of the United Democratic Front in South Africa." Diss., University of Pretoria, 2013. http://hdl.handle.net/2263/43307.
Full textDissertation (MA)--University of Pretoria, 2013.
lk2014
Political Sciences
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Rios, Flavia Mateus. "Institucionalização do movimento negro no Brasil contemporâneo." Universidade de São Paulo, 2009. http://www.teses.usp.br/teses/disponiveis/8/8132/tde-29102009-170307/.
Full textIn this dissertation I analyse the institutionalization of black movement in Contemporary Brazil. This social fact have been imply progressive professionalization of militants, more formal and bureaucratic organizations, new strategies to resource mobilizations and specialization of activism. The institutionalization is related to the way through the movement used the political opportunities offered by state and environment civil since the emergency of Brazilian democracy in the 1980s. The dynamic of organizations and the style of black protest, empiric objects of this work, express how the collective actions inside nowdays political context.
McDuffie, Scott Patterson. "James Lawson leading architect and educator of nonviolence and nonviolent direct action protest strategies during the student sit-in movement of 1960 /." NCSU, 2007. http://www.lib.ncsu.edu/theses/available/etd-03212007-153100/.
Full textChen, Yen-Hsin. "Protests in China: Why and Which Chinese People Go to the Street?" Thesis, University of North Texas, 2017. https://digital.library.unt.edu/ark:/67531/metadc984256/.
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Jämte, Jan. "Antirasismens många ansikten." Doctoral thesis, Umeå universitet, Statsvetenskapliga institutionen, 2013. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-81637.
Full textCastillo, Ulloa Ignacio [Verfasser], Angela [Akademischer Betreuer] Million, Simon [Akademischer Betreuer] Güntner, Angela [Gutachter] Million, and Simon [Gutachter] Güntner. "Space and radical planning: Linking protest action and local community self-development / Ignacio Castillo Ulloa ; Gutachter: Angela Million, Simon Güntner ; Angela Million, Simon Güntner." Berlin : Technische Universität Berlin, 2020. http://d-nb.info/1206245751/34.
Full textWright, Devon A. "Conservative Right-Wing Protest Rhetoric in the Cold War Era of Segregationist Mobilization." FIU Digital Commons, 2017. http://digitalcommons.fiu.edu/etd/3457.
Full textCaulfield, Thomas E. "Nonviolent Resistance to Security Policy in Nationalist Northern Ireland, 1970-1981." ScholarWorks, 2019. https://scholarworks.waldenu.edu/dissertations/6186.
Full textMbolela, Aura Yombo. "The relationship between organisational justice perceptions, organisational trust and willingness to engage in protest action for higher wages among low-income employees in South Africa." Master's thesis, Faculty of Commerce, 2021. http://hdl.handle.net/11427/32822.
Full textNtliziywana, Phindile. "Professionalisation of local government: Legal avenues for enforcing compliance with competency requirements." Thesis, University of the Western Cape, 2009. http://etd.uwc.ac.za/index.php?module=etd&action=viewtitle&id=gen8Srv25Nme4_6738_1284067820.
Full textThis study is a response to the dilemma of poor service delivery or the lack thereof. In this regard, this study posits the professionalisation of local government as part of the solution. The focus is on the administrative arm of local government, which is the major conduit for service delivery. Professionalisation of local government is a broader theme. For the present purposes, focus will be devoted to the competency component which entails attracting qualified personnel competent to discharge local government responsibilities. However, it is not limited to attracting already competent and professional staff. It also entails developing the skills of existing staff. This definition, in essence, relates to qualification through training, learning and specialisation.11 In essence, professionalisation of local government ensures that all employees act and behave in a professional way. In this regard, this study seeks to identify the competency standards set by the legislative framework and then explore the legal avenues for enforcing compliance, by the municipal administration, with such standards. This requires one to look at and answer the following questions: What constitutes municipal staff?
What is the content of the competency framework in question?
What are the enforcement mechanisms currently in place?
Whose role is it to enforce compliance with the competency framework?
Broadly speaking, enforcement can take two forms: hard enforcement and soft enforcement. The hard form of enforcement relates to giving incentives for compliance with the competency framework and dismissal for non-compliance. Softer enforcement, in turn, relates to correction and monitoring.
Almeida, Juniele Rabelo de. "Tropas em protesto: o ciclo de movimentos reivindicatórios dos policiais militares brasileiros no ano de 1997." Universidade de São Paulo, 2010. http://www.teses.usp.br/teses/disponiveis/8/8138/tde-12112010-150942/.
Full textThe purpose of this research is to look at the movement cycle of Brazilian military police demands which occurred at the end of the first semester of 1997. The police officers protests in Minas Gerais became a tactical banner for military police collective actions in various parts of Brazil. Fourteen states participated in the first national protest cycle: Alagoas, Bahia, Ceará, Goiás, Mato Grosso, Mato Grosso do Sul, Minas Gerais, Pará, Paraíba, Pernambuco, Piauí, Rio Grande do Sul; and, without an organized movement, São Paulo and Rio de Janeiro. Oral life history narratives revealed interactions between specific state-level military police groups and the nationally constituted organizational culture of the military police. Multiple issues of social movements and public safety in Brazil were addressed in four networks: 1st) Military Police in Minas Gerais: the beginning of the protest cycle cycle of protests; 2nd) Military Police of Alagoas, Ceará, Pernambuco and Pará: armed conflicts and threats; 3rd) Military Police of Paraíba, Bahia, Mato Grosso and Mato Grosso do Sul: encampments and negotiations; 4th) Military Police of Rio Grande do Sul, Piauí, Goiás, São Paulo and Rio de Janeiro: disciplined demonstrations and political articulation on the sidelines of the protest cycle. This analysis indicated different repertoires of collective action by the military police, which damaged the organizational elements, but not enough to undermine its institutional foundations. This research indicates possible connections between the organizational culture of the military police, expressed by the militarized precepts regarding institutional values and norms, and precepts of democratization prevalent in modern societies.
Zvoutete, Jackie Tatenda. "Impact of institutional diversity on unions and NGO's efforts to represent and articulate farm workers' grievances: case study of the 2012 Western Cape farm workers strike and protest action." Master's thesis, University of Cape Town, 2014. http://hdl.handle.net/11427/6824.
Full textBertz, Wågström Magda. "The Welfare State Upholders: Protests against Cuts in Sickness Benefits in Sweden 2006-2019 : A Case Study of Political Action against Welfare Retrenchment." Thesis, Uppsala universitet, Statsvetenskapliga institutionen, 2020. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-415482.
Full textAllsop, Geoffrey Charles. "Does the labour relations act unjustifiably limit the constitutional right of employees to freedom of assembly? Examining the constitutionality of the prohibition on purely political protest action and gatherings by off-duty employees over disputes of mutual interest." Master's thesis, Faculty of Law, 2019. https://hdl.handle.net/11427/31692.
Full textCarneiro, Olavo Brand?o. "TRATORA?O O ALERTA DO CAMPO: Um estudo sobre a??es coletivas e patronato rural no Brasil." Universidade Federal Rural do Rio de Janeiro, 2008. https://tede.ufrrj.br/jspui/handle/tede/655.
Full textConselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico
The purpose of this paper is to gain further understanding of the ways and mechanisms of social and political association that relate to dominant classes and groups in Brazil s countryside by looking at the strains, conflicts, and categories of the identities present at the gathering "Tratora?o - O Alerta do Campo". Organized by the Brazilian Confederation of Agriculture and Livestock (CNA) in June 2005. The Tratora?o mobilized large grain (soybean, corn and rice) and cotton farmers, especially in the Midwest and South areas of the country. Their main claim was to "renegotiate" agricultural debts, but they also demanded issues related to agripolitics, foreign trade and more room in State proceedings. A participative observation in the gathering together with the news broadcasted by CAN s Website comprised the main sources of our investigation, supplemented by interviews with rural and agro-industry business leaders and spokespeople, and surveys in the commercial media. The analysis allowed us to identify the street gathering as a recurrent political action of large rural entrepreneurs and large land owners, which expresses conflicts, strains, coalitions and intraclass and interclass agreements. It also allowed for the observation of how Brazil s rural patronage interests are built and represented as part of a class development process in which local bases manifestations, meetings of leaders from representative entities, and the initiatives of rural congressmen articulate progressively toward approaching the State.
Este trabalho procura melhor compreender as formas e mecanismos de organiza??o social e pol?tica das classes e grupos dominantes no campo brasileiro, a partir da observa??o das tens?es, conflitos, e ordena??es de identidades presentes na manifesta??o Tratora?o - O Alerta do Campo . Organizado pela Confedera??o Nacional da Agricultura e Pecu?ria do Brasil (CNA) em junho de 2005, o Tratora?o mobilizou agricultores de gr?os (soja, milho e arroz) e algod?o, especialmente das regi?es Centro-Oeste e Sul do pa?s. Sua principal reivindica??o foi a renegocia??o de d?vidas agr?colas, mas tamb?m demandavam quest?es relativas a pol?ticas agr?colas, com?rcio exterior e mais espa?o nas inst?ncias de Estado. A observa??o participante da mobiliza??o e as not?cias veiculadas pelo site da CNA constitu?ram as principais fontes da nossa investiga??o, complementadas por entrevistas com lideran?as e porta-vozes patronais rurais e agroindustriais e levantamentos na m?dia comercial. Nossa reflex?o permitiu identificar a manifesta??o de rua como uma a??o pol?tica recorrente dos empres?rios rurais e grandes propriet?rios de terra, construtora e express?o de conflitos, tens?es, alian?as e acordos intraclasse e entre classes sociais. O estudo permitiu a observa??o da constru??o e representa??o de interesses do patronato rural brasileiro como parte de um fazer-se classe, processo no qual manifesta??es locais das bases, reuni?es de dirigentes de entidades de representa??o, e iniciativas dos parlamentares ruralistas se articulam progressivamente para interpelar o Estado.
de, Rooij Eline A. "Specialisation of political participation in Europe : a comparative analysis." Thesis, University of Oxford, 2009. http://ora.ox.ac.uk/objects/uuid:d85dce69-2abe-44fa-ae1b-5a5c3f292c68.
Full textGiguere, Andrew M. ""...and never the twain shall meet:" Baltimore's east-west expressway and the construction of the "Highway to Nowhere."." Ohio : Ohio University, 2009. http://www.ohiolink.edu/etd/view.cgi?ohiou1243879048.
Full textHarazaki, Masashi. "Specific Recruitment of SPA-1 to the Immunological Synapse : Involvement of Actin-bundling Protein Actinin." Kyoto University, 2004. http://hdl.handle.net/2433/147566.
Full textMastnak, Lynne. "The process of engagement in non-violent collective action : case studies from the 1980s." Thesis, University of Bath, 1995. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.307114.
Full textFelgueiras, Sérgio Ricardo Costa Chagas. "A construção do protesto político: a geração à rasca." Doctoral thesis, Instituto Superior de Ciências Sociais e Políticas, 2014. http://hdl.handle.net/10400.5/12818.
Full textO protesto político é a manifestação do não-consentimento sobre uma decisão ou um conjunto de decisões políticas, ou o não reconhecimento da legitimidade para o processo de tomada de decisão, a participação política assume, assim, uma nova relevância para a cidadania política e social. A construção social do protesto político é um processo central às dinâmicas do sistemas social e político no estudo dos movimentos sociais. A investigação sobre o protesto político não-institucionalizado procura apreender a sua construção, isto é, a compreensão do fenómeno protesto é expandida para a compreensão do fenómeno da construção do fenómeno protesto abrindo, assim, novas possibilidades para o estabelecimento de linhas de investigação. Conceptualmente a resposta ao presente problema de investigação tem que ser procurada na agenda clássica do estudo dos movimentos sociais, sendo realizada a análise do evento de protesto a “geração à rasca” que ocorreu em Portugal no início do ano 2011, motivada pelo quadro cultural de acção colectiva da precariedade. A presente investigação utilizou metodologicamente a análise dos eventos de protesto expandida com a análise de conteúdo e o biograma
Political protest is the expression of the non-acquiescence about a decision or a set of political decisions, or the non-acknowledgement of the legitimacy for the decision making process. The political intervention is assumed to be, in that case, extremely relevant for the political and social awareness. The social construction of the political protest is a process within the dynamics of the political and social systems meander. The research of the uninstitucionalized political protest aims to comprehend its conception, that is, the very understandment of the protest phenomena shall be expanded to the understandment of its own construction, creating thus, new possibilities for the establishment of lines of investigation. Conceptually, the answer can be found within the roots of the classical study of the social movements, according to the analysis of the “geração à rasca” movement that took place in Portugal in the beginnings of 2011, which was motivated by a precarious cultural frame. Methodocagically, this research applied the analysis of the protest events, as well as the analysis of contents and the biogram
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Griaud, François. "Proteomic analysis of leukaemogenic protein tyrosine kinase action." Thesis, University of Manchester, 2012. https://www.research.manchester.ac.uk/portal/en/theses/proteomic-analysis-of-leukaemogenic-protein-tyrosine-kinase-action(ff9d490b-5a94-45fc-a857-4f0826e4a11a).html.
Full textOstrowski, Stephen M. "Pleiotropic mechanisms of statin action in Alzheimer's Disease." Cleveland, Ohio : Case Western Reserve University, 2007. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=case1190669698.
Full textHeesom, Kate J. "The regulation of acetyl-CoA carboxylase by insulin in adipose tissue." Thesis, University of Bristol, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.294552.
Full textDash, Shantoshini. "Role of M1 protein and actin-associated cellular cofactors in Influenza A Virus assembly and release." Thesis, Montpellier, 2017. http://www.theses.fr/2017MONTT034.
Full textThe influenza A(H1N1)pdm09 virus, commonly known as swine flu, caused the very first pandemic of 21st century. Influenza virus, an enveloped RNA virus, uses the host cellular machinery for its assembly and release from the host cell plasma membrane. In this study, we were interested in the role of the viral M1 matrix protein in this process. M1 is the most abundant and vitally important protein present in influenza virus. The N-terminal 164 residues of M1 protein comprise of two basic domains which are the arginine triplet (R76/77/78) on helix 5 and the nuclear localization signal on helix 6, which are very well conserved among the influenza A virus subtypes. Firstly, to study M1-membrane interaction, we developed and standardized a minimal system consisting of M1+M2+NS1/NEP(±M) in which we could also observe production of VLPs incorporating M1. Using this system, we performed mutations in the M1 arginine triplet and looked at changes in M1 membrane attachment and M1 incorporation in VLP. As a result of these mutations, the M1 protein remained cytosolic and there was a drastic reduction in M1 containing VLP release. Mutating the entire arginine triplet to an alanine triplet inhibited VLP production completely. Also, a mutant virus with this alanine triplet failed completely to produce infectious virions. Thus we established the importance of the arginine triplet in M1 membrane attachment and virion production. Consequently, to study manipulation of actin and its cofactors by the virus, we used siRNA mediated gene silencing in the VLP producing minimal system. We observed a reduction in M1 containing VLP production upon inhibition of Rac1 and enhancement of M1 containing VLPs released upon inhibition of RhoA and Cdc42. By using an IAV (H3N2)-nanoluciferase virus on pulmonary A549 cells, we studied effect of depletion of RhoGTPases and their effectors on virus production. We observed that along with Rac1, inhibition of Wave2 and Arp3 also reduces the infectivity of H3N2 virus at the late phase of infection without any effect on the early phase of infection. The proteins interacting with M1 were identified by LC-MS/MS and included cofilin and annexin A2. Cofilin, already known to take part in the actin reorganization during the late phase of influenza A virus infection, is also one of the downstream effector linked to Rac1, Wave2, Pak1 and LIMK, for lamellipodia formation. Annexin A2 is also known to sequester PS at the inner leaflet of the cell plasma membrane. The viral protein M1 is able to recognize these clusters of PS, which ultimately initiates the viral assembly process. Thus, our results, while defining the mechanism of M1 membrane attachment, also indicate the possible involvement of Rac1, Wave2 and Arp3 as pro-viral factors in IAV assembly and release
Adusei-Danso, Felix. "Systematic approach to protein crystallization :emphasis on Vaccinia virus complement control protein (VCP)." Thesis, University of the Western Cape, 2006. http://etd.uwc.ac.za/index.php?module=etd&action=viewtitle&id=gen8Srv25Nme4_4781_1188474852.
Full textThis work examined the systematic approach to protein crystallization, exploring some of the techniques that have been developed to enhance the success rate of crystallization. The work was centered on two proteins
namely Vaccinia virus complement control protein (VCP) and glutamate dehydrogenase (GDH) from Bacteriodes fragilis. The crystal structures of the full lengthe native VCP and VCP bound to heparin had already been determined. In the same way, the structure of GDH from Bacteriodes fragilis is not known, even though structures of other GDHs from different organisms have been determined.
Leo, Vincenzo Carlo. "The mechanism of action of a mutant mitochondrial fission protein." Thesis, University of Leeds, 2013. http://etheses.whiterose.ac.uk/5052/.
Full textHolmes, Peter. "Structure and mode of action of the TolA-TolB complex from Pseudomonas aeruginosa." Thesis, University of Oxford, 2016. https://ora.ox.ac.uk/objects/uuid:cccb0c88-5c89-4d21-81eb-70ebf513c7ab.
Full textAlhabashneh, Mohammad Abad Alhameed. "Protect team handler (PTH)." Thesis, University West, Department of Technology, Mathematics and Computer Science, 2006. http://urn.kb.se/resolve?urn=urn:nbn:se:hv:diva-567.
Full textThere is always a need for easy-to-follow processes that enable accurate and non-time consuming solutions. Nowadays we see a lot of different approaches to development processes in software engineering. This project is concerned with how to manage a software development process in a reliable, secure and efficient way. Software is available which provides some help for project managers / administrators to work more productively, with effective communication. Using such systems, it is possible to keep track of all the phases of development, including task distribution, making maximum use of previous hands-on experience and increasing productivity, to deliver a finished product in minimum time. No existing solution, however, fulfills all the desirable criteria. This paper describes the motivation, design and implementation of an improved development management system using Active Server Pages and Microsoft Internet Information Services with a backend Microsoft Access Database developed using a waterfall software development process. The resulting system is described and evaluated. This system will be beneficial for software houses, because they can communicate on the web, allowing efficiency gains by avoiding the need to call meetings for distribution of tasks among employees, with the additional advantage of location-transparent team management through the Internet.