Dissertations / Theses: 'Protest song'

1

Galdino, Roberto Carlos. "A porta da saida : a poetica das canções de Torquato Neto." [s.n.], 2008. http://repositorio.unicamp.br/jspui/handle/REPOSIP/270028.

Full text

Abstract:

Orientadores: Luiz Carlos da Silva Dantas, Alexandre Soares Carneiro Dissertação (mestrado) - Universidade Estadual de Campinas, Instituto de Estudos da Linguagem Made available in DSpace on 2018-08-11T03:01:29Z (GMT). No. of bitstreams: 1 Galdino_RobertoCarlos_M.pdf: 566706 bytes, checksum: ffa65cd4536e53831ec5f0e219a3f819 (MD5) Previous issue date: 2008 Resumo: Nosso estudo debruça-se por sobre as canções de Torquato Neto, anteriores a sua participação no Tropicalismo, que tem um perfil semelhante à canção engajada, de protesto, de meados da década de 1960, e durante seu envolvimento com o movimento, tomando-as como uma entrada para o questionamento dos discursos críticos estabelecidos sobre a sua poética. Procuramos estabelecer um diálogo entre a nossa escuta das canções e as leituras críticas que as tomam isoladamente, como texto poético apenas. De modo a questionar os dois pólos extremos em que é confinada sua poética: Ou o poeta é visto enquanto um artista de vanguarda continuador de uma certa tradição do novo, de inventividade e rigor construtivo; ou como romântico libertário radical, que por coerência com seu projeto poético-existencial, renuncia a vida Abstract: Our study bends over Torquato Neto¿s songs, prior to his participation at the Tropicalism, which has a similar profile of the activist song, of protest, in the middle of the 1960s, and during his involvement with the movement, turning them into a beginning of questioning the established critical discourses about his poetry. We try to establish a dialogue between our listening to the songs and the critical readings which consider them separately, as a poetic text only. So that we can question the two opposite extremes in which his poetry is confined to: either the poet is seen as an avant-garde artist who continues a certain tradition again, of ingenuity and constructive rigidity, or as a romantic radical libertarian who, in accordance with his political-existential project, abandons his life Mestrado Literatura Brasileira Mestre em Teoria e História Literária

APA, Harvard, Vancouver, ISO, and other styles

2

Rose, Mark. "Striking a discordant note : protest song and working-class political culture in Germany, 1844-1933." Thesis, University of Southampton, 2010. https://eprints.soton.ac.uk/367356/.

Full text

Abstract:

This thesis examines the role played by protest song in the development of the political culture of Germany’s industrial working class between 1844 and 1933. Protest song was an integral component in the struggle of the German working class to achieve some measure of political and social equality in the nineteenth and early twentieth centuries. Throughout this period, the working class found itself subjected to varying levels of political repression by the German authorities, and in order to promote their political views, industrial workers used the medium of song to protest against injustice. The thesis seeks to determine the significance of protest song for the political development of the German industrial working class through an analysis of song lyrics. The study of working-class protest song lyrics has largely been the preserve of historians from the former German Democratic Republic, where scholarship was shaped by the unique political imperatives of history writing under the Communist regime. This thesis seeks to redress the historiographical imbalance that this approach engendered, arguing that protest song produced under the auspices of the Social Democrats was both a culturally valid and politically significant feature of German working-class political life, albeit one that offered a different ideological approach to that of the overtly revolutionary output of the Communist movement. Additionally this thesis will acknowledge that working-class song was not merely used as an instrument of protest, but also as a medium to communicate political ideology. Protest song was an integral part of the cultural capital of the working class milieu, creating a distinct canon upon which German industrial workers drew in a variety of political, social and cultural situations. This study will engage with this canon in order to establish how the cultural practice of singing endowed working-class protest songs with an intrinsic political significance.

APA, Harvard, Vancouver, ISO, and other styles

3

Chouana, Khaled. "Vers une redéfinition de la protest song ? Réflexions sur la chanson contestataire aux États-Unis : le cas de Bruce Springsteen et la reflective song." Thesis, Montpellier 3, 2018. http://www.theses.fr/2018MON30005/document.

Full text

Abstract:

Ce travail constitue une réflexion sur la chanson contestataire contemporaine aux États-Unis à travers les chansons et l’engagement politique et social de Bruce Springsteen. Nous défendons la thèse que Springsteen renouvelle le genre de la protest song en s’adaptant à l’époque contemporaine. Pour ce faire, le chanteur adopte quatre stratégies artistiques. Elles consistent à chanter sur les gens ordinaires et leur quotidien, aborder le désenchantement et le désespoir des plus exclus, composer des chansons qui remplissent une fonction cathartique en ayant recours à l’imitation et enfin à militer dans la sphère politique et sociale en soutenant des candidats lors des élections présidentielles américaines tout en offrant des dons aux organisations caritatives qui aident les plus démunis. La thèse démontre que Springsteen arrive à se hisser au sommet du classement des meilleures ventes aux États-Unis et à travers le monde grâce à ces stratégies. Elles lui ont permis de mobiliser un public et d’avoir des millions de fans. Nous proposons d’appeler les chansons engagées de Springsteen des chansons méditatives (reflective songs). La chanson méditative de Springsteen est, sans doute, l’outil culturel de contestation le plus adapté à l’époque contemporaine où il est difficile pour un artiste engagé de protester efficacement du fait de la marchandisation de l’acte même de sa contestation. Il se peut que la reflective song de Bruce Springsteen ne change pas le monde, mais elle peut en revanche permettre aux gens de croire en un monde meilleur et donc de les dissuader de se révolter This work is a study of contemporary protest song in the USA through the songs and social and political activism of Bruce Springsteen. It contends that Springsteen has been able to reexamine American protest songs and frame a new genre under the umbrella of rock music by adapting his music to the social and political context of contemporary America. The thesis that I defend shows that the reason behind the commercial success of Springsteen can be explained by the fact that he has adopted several artistic strategies which have brought him an audience composed of dedicated fans who admire him. I argue that Springsteen adopts four main strategies: firstly, singing about ordinary people and reporting their daily hardships; secondly, composing somber songs that deal with the despair of blue collars and marginalized Americans; thirdly, adopting mimesis (imitation) which has a cathartic effect on Springsteen’s audience; and finally, getting involved in social and political activism. The thesis contends that Springsteen is among the top selling rock singers in the USA and several other countries thanks to these four strategies. Springsteen has reinvented protest song as a genre and has become a reference in a world where everything is co-opted including the very act of rebellion. I, therefore, suggest a new term, reflective songs, to describe the songs of Bruce Springsteen when they reflect on the condition of the socially excluded instead of protesting pointlessly. Springsteen’s reflective song is undoubtedly the most relevant artistic medium to shed light on the most deprived people of contemporary America. Perhaps, the reflective song of Bruce Springsteen will not change the world, but at least it gives people a reason to hope for a better future

APA, Harvard, Vancouver, ISO, and other styles

4

Marchbanks, Jack R. "Pride and Protest in Letters and Song: Jazz Artists and Writers during the Civil RightsMovement, 1955-1965." Ohio University / OhioLINK, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1522929258105629.

Full text

APA, Harvard, Vancouver, ISO, and other styles

5

Sorroce, Danilo Sérgio. "A canção de protesto e o rock protesto: diálogos da democracia brasileira." Universidade Presbiteriana Mackenzie, 2015. http://tede.mackenzie.br/jspui/handle/tede/2357.

Full text

Abstract:

Made available in DSpace on 2016-03-15T19:47:39Z (GMT). No. of bitstreams: 1 Danilo Sergio Socorre.pdf: 1109484 bytes, checksum: 8b5fac3c6375180970eb16207e1d3cb0 (MD5) Previous issue date: 2015-02-19 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior During this study we focused our research on dialogues between the Protest Song of 1960s and 1970s, especially those occurred in certain works created by Gilberto Gil, Caetano Veloso and Chico Buarque de Holanda and a few songs produced by politicized and protest rock launched by bands from Brasilia such as Capital Inicial, Plebe Rude and Legião Urbana in the 1980s, contextualizing this cultural production within an important period in the recent history of Brazil. Through the theory developed by Bakhtin, we were confronted with dialogues between speakers and speeches, which allowed us to produce analysis of the characteristics and content of the dialogues between the "Old" Protest and the "New" Protest. The investigation allowed us to point out some similarities between the songs analyzed, and also enabled us to interpret them as articulating elements of the period in which they arose, which is evident in the performance of songs from both periods as a political tool of contestation. No decorrer deste estudo, procuramos focar nossa investigação sobre os diálogos estabelecidos entre a Canção de Protesto dos anos 1960 e 1970, em especial, os ocorridos em determinadas obras criadas por Gilberto Gil, Caetano Veloso e Chico Buarque de Holanda e algumas canções produzidas pelo rock politizado e de protesto lançado pelas bandas de Brasília: Capital Inicial, Plebe Rude e Legião Urbana, nos anos 1980. Assim, pudemos contextualizar a produção cultural, dentro desse período importante da história recente do Brasil. Através da teoria desenvolvida por Bakhtin, foram confrontados os diálogos entre interlocutores e discursos, o que nos permitiu produzir análises acerca das características e conteúdos dos diálogos travados entre o Velho Protesto e o Novo Protesto. A pesquisa admitiu apontar algumas convergências entre as canções analisadas, bem como, também, possibilitou-nos interpretá-las como elementos articuladores do período em que surgiram que, por conseguinte, evidencia-nos a atuação das canções de ambos os períodos como instrumento político de contestação.

APA, Harvard, Vancouver, ISO, and other styles

6

Bohlman, Andrea Florence. "Activism and Music in Poland, 1978-1989." Thesis, Harvard University, 2012. http://dissertations.umi.com/gsas.harvard:10516.

Full text

Abstract:

This dissertation presents a historical study of intersections between music and activism in Poland from the election of Cardinal Karol Wojtyła as Pope John Paul II in 1978 to Poland’s first democratic elections in 1989. Musical action in three cultural spheres shapes the project: (1) the political activism of musicians, (2) activists who turn to music as a political instrument, and (3) the musical ambitions of the communist authorities, the Polish United Workers’ Party. I critique the repercussions of politics in music as well as music’s significance for policy makers and dissidents, and I assume that neither course of influence is intrinsic or inevitable under state socialism. In doing so, I highlight the complex relationship between activist culture and music at the end of the Cold War. Throughout the decade, religious hymns, patriotic anthems, experimental music, and popular songs shared spaces in Polish society, projected analogous ambitions, reflected communal responses, and partook in debates about culture’s capacity to effect political action. The plurality of musical genres and music histories during the Cold War reflects the political tensions in the Polish opposition to state socialism. The diverse materials I investigate in this dissertation respond both to the tumultuous politics of the 1980s and to the ethnographic, historical, and analytical methods I employ to write music history. My thesis—that political activism offered politicians, activists, and musicians the opportunity for constructive creative action—provides a model for rethinking Cold War music history. I begin with an explanation of the Communist Party’s program for music and the practical means by which it carried out this vision through the decade. Two chapters examine specific historical moments: I critique the ways in which music has come to be associated with the August 1980 strikes that brought about the formation of Solidarity, the first independent trade union in the Eastern bloc, and map the sites of music making in the weeks after martial law’s imposition in December 1981. I explore the resonance of popular sacred hymns and plainchant for musicologists, composers, and members of the opposition through the final decade of the Cold War. The dissertation concludes by analyzing the unofficial musical discourse on independence, drawing out the concept’s resonance for artists invested in their own musical autonomy. Music

APA, Harvard, Vancouver, ISO, and other styles

7

Hyder, Thomas. "The Legacy of Civil Rights Protest Music: Sweet Honey in the Rock's "The Ballad of Harry T. Moore"." Master's thesis, University of Central Florida, 2012. http://digital.library.ucf.edu/cdm/ref/collection/ETD/id/5314.

Full text

Abstract:

This study investigates the role music played in the Civil Rights Movement as a form of political protest. The first part of the studies analyzed how political protest music was used in the early part of the twentieth-century leading up to the Civil Rights Movement of the 1960s. An analysis of the role of music in African-American culture also provides a historical background to the music-making of the Civil Rights Movement. Specific musical forms such as topical ballads, freedom songs, and spirituals are examined. In addition, musical influences of African culture as well as religious influences on music-making during the Civil Rights Movement are also examined. The second section of the paper investigates the life and murder of NAACP organizer Harry T. Moore of Mims, Florida. Moore's life and death became the subject of a topical ballad, “The Ballad of Harry T. Moore”, composed in 2001 by musical group Sweet Honey In The Rock. An analysis of the song's, literary, political, and musical connections to the ideology and music of the Civil Rights Movement, as well as subject matter, gives evidence that places the song within the tradition of the musical protest activities of the Civil Rights Movement. M.A. Masters Music Arts and Humanities Music

APA, Harvard, Vancouver, ISO, and other styles

8

Gomes, Caio de Souza. "\"Quando um muro separa, uma ponte une\": conexões transnacionais na canção engajada na América Latina (anos 1960/70)." Universidade de São Paulo, 2013. http://www.teses.usp.br/teses/disponiveis/8/8138/tde-28062013-130124/.

Full text

Abstract:

O objetivo desta pesquisa é analisar como, ao longo das décadas de 1960 e 70, artistas ligados à canção engajada conceberam projetos de integração da América Latina por meio da canção, e como esses projetos resultaram no estabelecimento de uma série de conexões transnacionais. Neste período, os impactos da Guerra Fria tiveram como consequência um contexto político fortemente polarizado, o que afetou diretamente o campo cultural e, especialmente, o campo artístico. As experiências políticas comuns favoreceram as trocas de ideias e a intensa circulação de artistas por todo o continente, permitindo o estabelecimento de uma série de contatos e diálogos. Utilizando como fonte principal a discografia dos mais destacados artistas de cada país, o objetivo á analisar essa história de conexões transnacionais na canção engajada latino-americana focalizando três fases distintas: a) a primeira, entre 1963 e 1966, momento de formação e consolidação dos movimentos de nueva canción no Cone Sul, em que o engajamento se manifestou fundamentalmente por meio da crítica social; b) a segunda, entre 1967 e 1969, marcada pelos impactos do I Encuentro de la Canción Protesta, realizado em Cuba, que resultou no destaque de dois temas importantes: a revolução e o anti-imperialismo; c) a terceira, que compreende a década de 1970, período marcado pela radicalização dos discursos políticos, pela intensificação das conexões e também pelas tentativas de inserção dos artistas brasileiros nos circuitos da canção engajada latino-americana. The objective of this research is to analyze the Latin America integration project conceived by the artists involved with the protest song in the 1960s and 1970s. In this period, the impact of Cold War resulted in a polarized debate that affected the cultural field, and especially the artistic field. The common political experiences favored the exchange of ideas and the intense movement of artists across the continent. Using the most prominent artists discography as source, the purpose is to analyze three moments of the history of \"transnational connections\" in the protest song in Latin American: a) the first, between 1963 and 1966, time of development and consolidation of nueva canción movement in the Southern Cone, when the engagement was manifested fundamentally by social criticism; b) the second, between 1967 and 1969, marked by the impact of the I Encuentro de la Canción Protesta held in Cuba, that highlighted two important themes: revolution and anti-imperialism; c) the third, which comprises the 1970s, a period marked by the radicalization of political discourse and by the intensification of connections. Especially in this third phase, I analyze the Brazilian artists efforts to integrate the circuits of Latin American protest song.

APA, Harvard, Vancouver, ISO, and other styles

9

Santos, Adna Nascimento Alves. "A compreensão de implícitos textuais nas canções de protesto social : uma alternativa na formação do leitor crítico." Universidade Federal de Sergipe, 2015. https://ri.ufs.br/handle/riufs/6446.

Full text

Abstract:

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior It has become imperative in the current context of Brazilian public school the reading practices as an agenda of constant reflection and research for alternatives that can contribute to the development of the reading comprehension in various levels of middle school students, given the Saeb data and Prova Brasil (2014), whose large-scale assessments showed difficulties of the students to select implicit information, inferring the meaning of a word or expression, among others; there by, indicating, serious deviations of understanding and the appropriation of the writing production, in the act of reading. Based on this fact, the present report has as a guiding objective to promote the formation of a proficient and critical reader through the analysis of Brazilian social protest songs in activities of reading and understanding the implicit textual, enabling recognize them as situated discursive practices in daily life that can contribute to the formation of active citizens in society. Was taken as theoretical support the studies of textual linguistics, the sociocultural perspective; using the sociointeractional language design, subject and text for which the textual and discursive understanding can only be understood when it exceeds the limits of decoding linguistic elements and reaches the horizons of mobilization of the socio-historical and cultural knowledge and reconstruction of these within a communication situation in a given context. Therefore, we used a qualitative research, with the methodological approach by the application of didactics sequence in a 9th grade of elementary school, that used the song of social protest genre as mediator. Taking into account the proposal prepared for the research and what was executed, it is possible to say that the understanding of the text work from the songs analysis is configured as a positive and effective alternatives, as the students found new forms of meanings construction for the studied texts. The results obtained from this work fulfilled the ultimate goal of this trajectory that is becoming theoretical and practical support for the development of a concrete tool for socialization of experienced knowledge: the Pedagogical Section, whose main purpose is to share successful experiences with other Portuguese-speaking teachers in teaching and in the reading learning process. Tornou-se imperativo, no atual contexto da escola pública brasileira, que as práticas de leitura sejam pauta de constante reflexão e da busca por alternativas que colaborem para o desenvolvimento da compreensão leitora dos alunos do ensino fundamental (EF), em vários níveis, haja vista os dados do Saeb e da Prova Brasil (2014), cujas avaliações em larga escala apontaram dificuldades dos discentes para selecionar informações implícitas, inferir o sentido de uma palavra ou expressão, entre outros; indicando, desse modo, os graves desvios de compreensão e apropriação da produção escrita, no ato de ler. Partindo dessa realidade, o presente relatório tem como objetivo norteador promover a formação de um leitor proficiente e crítico por meio da análise de canções brasileiras de protesto social em atividades de leitura e compreensão dos implícitos textuais, possibilitando reconhecê-las como práticas discursivas situadas no cotidiano que contribuem com a formação de cidadãos ativos na sociedade. Tomaram-se como aporte teórico os estudos da Linguística Textual, na perspectiva sociocultural; adotando-se a concepção sociointeracional de língua, de sujeito e de texto para a qual a compreensão textual e discursiva só pode ser entendida quando ultrapassa os limites da decodificação de elementos linguísticos e alcança os horizontes da mobilização dos saberes sociohistórico e cultural e da reconstrução destes no interior de uma situação de comunicação, num dado contexto. Para tanto, adotou-se a pesquisa de cunho qualitativo, tendo como caminho metodológico a aplicação de Sequência Didática em uma turma de 9º ano (EF), que utilizou a canção de protesto social como gênero textual mediador. Levando-se em conta a proposta elaborada para a pesquisa e o que foi executado, é possível dizer que o trabalho de compreensão do texto a partir da análise de canções configurou-se como uma alternativa positiva e eficiente, na medida em que os alunos descobriram novas formas de construção de sentidos para os textos estudados. Os resultados obtidos a partir desse trabalho atendem ao objetivo final dessa trajetória, que é a elaboração de um instrumento concreto de socialização dos conhecimentos teórico-práticos vivenciados: o Caderno Pedagógico, cujo principal propósito é compartilhar experiências exitosas, testadas no ensino e na aprendizagem da leitura, com outros professores de língua portuguesa.

APA, Harvard, Vancouver, ISO, and other styles

10

Tabti-Kouidri, Fatiha. "La chanson algérienne des années 1990, un vecteur de contestation et de diversité : langage, langues et enjeux socioculturels." Thesis, Paris 3, 2009. http://www.theses.fr/2009PA030014.

Full text

Abstract:

L’objet de ces recherches est d’étudier un moyen d’expression, de communication, de résistance, de mobilisation, de contestation, de revendication et d’affirmation identitaire, qui, dans une Algérie aux multiples facettes et aux diverses attaches où le substrat culturel est massivement investit par l’oralité, a constitué pour une grande partie de la population algérienne, durant les années 1990, un exutoire indispensable au maintient d’un équilibre individuel et collectif fortement compromis par un déferlement de violence sans précédent dans l’histoire de l’Algérie indépendante, une possibilité non négligeable d’affirmation de soi mais aussi et surtout une tribune d’où s’est élevé un discours populaire de substitution au discours dominant du pouvoir et celui dominateur des extrémistes religieux : la chanson. En effet, charriant les sentiments et les ressentiments d’une population prise en étau entre terrorisme et répression et dont la parole est exclue des circuits officiels, transgressant les interdits, réfutant l’uniformisation culturelle officiellement imposée en revendiquant une culture et une identité plurielles, les chansons algériennes des années 1990 se présentent comme un vecteur majeur de contestation et de diversité, un moyen de communication à part entière qu’il s’agit d’appréhender en le reliant à l’ensemble du système dont il participe The object of those researches is to study in a country where the cultural substratum is massively invested by the oral character, a way of expression, of communication, of resistance, of mobilization, of contestation, of claiming and affirmation of their identity, that was constituted by a great part of the Algerian population, an indispensable outlet to the maintenance of an individual and collective equilibrium strongly compromised, but also and mostly a non negligible possibility of self affirmation : the song. In deed, carrying feelings and resentments of a population taken between terrorism and repression and whose speech is excluded of the official circuits, denouncing the violence of the religious extremism like the abuses and the deficiencies of the power in place, transgressing the interdicts and the taboos, claiming a plural culture and identity, the Algerian songs of the 1990 are a vector of contestation and diversity, a real way of communication that we have to apprehend by linking it to the whole system of which it participates

APA, Harvard, Vancouver, ISO, and other styles

11

Roman-Rivera, William J. "Silence and screams "Nueva Canción" and its impact on political movements in Chile, Argentina and Uruguay /." Muncie, Ind. : Ball State University, 2008. http://cardinalscholar.bsu.edu/383.

Full text

APA, Harvard, Vancouver, ISO, and other styles

12

Tiné, Paulo José de Siqueira. "Procedimentos modais na música brasileira: do campo étnico do Nordeste ao popular da década de 1960." Universidade de São Paulo, 2009. http://www.teses.usp.br/teses/disponiveis/27/27157/tde-13122009-102355/.

Full text

Abstract:

a)Objetivos: Traçar um perfil dos procedimentos melódicos modais presentes nas manifestações étnicas e populares do nordeste brasileiro afim de verificá-los em autores ligados à música popular do Brasil na década de 1960, cotejando os diferentes procedimentos harmônicos realizados. b)Métodos: O principal método utilizado foi o da análise a partir da transcrição de exemplos em áudio. A partir dessas análises uma série de comparações com materiais similares encontrados na bibliografia foi realizada. c) Resultados: Como resultado dessa pesquisa foram apontados os principais modos e traços cadenciais utilizados no repertório étnico e popular escolhido do nordeste brasileiro. Esses traços foram importantes na construção das canções aqui selecionadas, características da chamada MPB da década de 1960 apresentando, a partir dessas análises, os principais procedimentos harmônicos desses autores relacionados aos modos e traços cadenciais encontrados. e)Objectives: Delineating a profile of the modal melodic procedures present in the ethnic and popular manifestations of the Northeast region of Brazil in order to verify them in authors connected to the Brazilian popular music from the 1960s, comparing the different harmonic procedures produced. f)Methods: The main method used was the analysis of transcripts of audio samples. From these analyses a series of comparisons with similar materials found in the bibliography was made. g)Results: As a result of this research, the main modes and cadential traces used in the ethnic and popular repertoire chosen from the Northeast of Brazil were indicated. These traces were important in the construction of the songs here selected, characteristics of the so-called MPB of the 1960s presenting, from those analyses, the main harmonic procedures of those authors related to the cadential modes and traces found.

APA, Harvard, Vancouver, ISO, and other styles

13

Corneloup, Bernard. "Génération MPB : filiations, dialogues et ruptures : Mouvements musicaux et dynamique socioculturelle dans le Brésil contemporain." Thesis, Lyon 2, 2012. http://www.theses.fr/2012LYO20087/document.

Full text

Abstract:

1958, au Brésil, surgit un genre musical nouveau, la Bossa Nova, qui s'annonce comme une révolution dans le paysage musical traditionnel. Vingt ans plus tard, 1978 est l'année de "l'Ouverture" politique, Chico Buarque, poète musicien qui a dominé la décennie autant à travers ses chansons qu'à travers son attitude de résistance contre la dictature, lance un disque dans lequel il enregistre des musiques jusqu'alors interdites par la censure. Cet événement conclut une période de grande créativité artistique, ponctuée de bouleversements sociaux et culturels, de déclarations d'intentions, de ruptures, de prises de position en rapport avec la vie politique ou culturelle du pays, qui souvent ne fait qu'une. Ces vingt années ont vu apparaître les figures les plus marquantes de la chanson brésilienne du XXe siècle, en même temps que le sigle par lequel la chanson urbaine a reçu ses lettres de noblesse : MPB, initiales de Música Popular Brasileira. S'ancrant dans la tradition tout en s'en défendant, dialoguant avec les influences extérieures qu'elle influence également, se démarquant jusqu'à la rupture des mouvements qui lui étaient les plus proches, cette génération nous apparaît aujourd'hui beaucoup plus unie et limitée dans le temps qu'elle n'en avait conscience. C'est pourquoi le temps est venu, avec le recul historique, de s'interroger avec un regard nouveau sur la production musicale de cette époque, afin d'en définir les caractéristiques qui la soudent et la distinguent à la fois In 1958, a new musical genre appears in Brazil: the Bossa nova, which looks like a revolution in the traditional musical landscape. Twenty years later, 1978 is the year of political openness, Chico Buarque, poet musician who dominated the decade through his songs as well as his resistance against the millitary dictatorship, launches an album on which he records some songs that were censored until then. This event puts an end to a period of great artistic creativity, punctuated with social and cultural changes, statements of intents, ruptures, standpoints on the political or cultural life of the country, which often are one. These twenty years witnessed the appearance of the most striking figures of Brazil’s 20th century music scene, and of the acronym whereby the urban song gained respectability: MPB, initials of Música Popular Brasileira. Rooted in tradition though defending itself from it, exchanging with outside influences which it influences as well, distancing itself till the rupture of the movements of which it was the closest, today this generation seems to us much more united and limited in time than it thought it was. That’s why the time has come, with historical perspective, to look at the musical production of that period with fresh eyes, in order to define the characteristics that unite and distinguish it at the same time 1958, nasce no Brasil un gênero musical novo, a Bossa Nova, que se anuncia comme uma revolução na paisagem musical. Vinte anos depois, 1978, é o ano da "Abertura" política, Chico Buarque, poeta músico que dominou a década tanto pelas suas canções como pela sua atitude de resistência contra a ditadura, lança um disco no qual ele grava músicas até então proibidas pela censura. Esse acontecimento conclui um período de intensa criatividade artística, acompanhada por grandes mudanças sociais e culturais, declarações de intenções e rupturas, tomadas de posição em relação com a vida política ou cultural do país, que muitas vezes faz uma só. Esses vinte anos viram emergir as figuras mais marcantes da canção brasileira do século XX, concomitantemente à sigla pela qual a canção urbana moderna foi consagrada : MPB, iniciais de Música Popular Brasileira. Ancorando-se na tradição sem reivindicá-lo, dialogando com as influências externas que ela influencia por sua vez, demarcando-se até à ruptura dos movimentos que lhe eram próximos, essa geração nos aparece hoje muito mais unida e circunscrita no tempo do que ela o percebia. Por essa razão, o tempo veio, com o recuo histórico, de questionar com um olhar novo a produção musical daquela época, para definir as características que a unificam e distinguem ao mesmo tempo

APA, Harvard, Vancouver, ISO, and other styles

14

Ritter, Jonathan Larry. "A river of blood music, memory, and violence in Ayacucho, Peru /." Diss., Restricted to subscribing institutions, 2006. http://proquest.umi.com/pqdweb?did=1273100121&sid=1&Fmt=2&clientId=1564&RQT=309&VName=PQD.

Full text

APA, Harvard, Vancouver, ISO, and other styles

15

Carr-Wilcoxson, Amanda Marie. "Protest Music of the Vietnam War: Description and Classification of Various Protest Songs." Digital Commons @ East Tennessee State University, 2010. https://dc.etsu.edu/etd/1686.

Full text

Abstract:

The Vietnam War and subsequent protest movement remains one of the most tumultuous times in U.S. history. This thesis is an attempt to define and describe the protest movement as well as the varied popular protest songs that came from this era. Building on a previous study written by Elizabeth Kizer, this thesis creates sub-categories in which the protest music falls into. The first two chapters of this study help by giving historical context to the songs by describing the Vietnam War and then the protest movement in the U.S. The final chapter then deals with popular protest songs that appeared on the Billboard charts between 1960 and 1969.

APA, Harvard, Vancouver, ISO, and other styles

16

Lanci, Michael P. "Songs for Joe Hill." University of Cincinnati / OhioLINK, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=ucin149580760300849.

Full text

APA, Harvard, Vancouver, ISO, and other styles

17

Yancey, Eddie. "Rhythm and Blues protest songs: voices of resistance." DigitalCommons@Robert W. Woodruff Library, Atlanta University Center, 2007. http://digitalcommons.auctr.edu/dissertations/610.

Full text

Abstract:

This study was an investigation of seven selected rhythm and blues black protest songs, from 1964 to 1975, and their relationship to black empowerment during the era of the Black Power Movement. The songs were analyzed using content analysis and revealed three expected themes of self/black identity, racial equity and self-determination. A fourth theme, social change, evolved during the study. The study focused on the political, cultural and economic ramifications, especially of black music, as a form of protest against a system that proved to be one of inequality, and prejudicial segregation in every aspect of the black life. The rhythm and Blues black protest songs were seen as efforts to empower the black community to take responsibility for its own survival. Of the seven artists who participated in the Black Power Movement, Nina Simone, Curtis Mayfield and Sam Cooke were the most prolific musical contributors to the protest movement of Student Nonviolent Coordinating Committee (SNCC) in its efforts to deal with segregation. The four musicians wrote and performed music that was geared specially for transformations in social justice and to engage the American society, both black and white people, in substantial and lasting social change. The music of the other artists was instrumental in bringing Rhythm and Blues into a revolution. The musical revolutionaries were James Brown, Marvin Gaye, Aretha Franklin, and Stevie Wonder. The analysis, however, did not reveal whether or not the rhythm and blues black protest songs contributed to or led to black empowerment during the Civil Rights Black Power era.

APA, Harvard, Vancouver, ISO, and other styles

18

Marcato, Silvia <1988&gt. "A sociological approach to The Beatles' protest songs." Master's Degree Thesis, Università Ca' Foscari Venezia, 2013. http://hdl.handle.net/10579/3353.

Full text

APA, Harvard, Vancouver, ISO, and other styles

19

Gramani, Giuliana. "Mudanças e permanências: a indústria cultural e os anos 60 em I\'m Not There, de Todd Haynes." Universidade de São Paulo, 2014. http://www.teses.usp.br/teses/disponiveis/8/8147/tde-06112014-115951/.

Full text

Abstract:

O presente trabalho tem por objetivo analisar o filme Im Not There (Não estou lá, EUA, 2007), dirigido pelo norte-americano Todd Haynes. A análise terá como seu principal objetivo detectar o tema central da obra, que retrata a vida e carreira de Bob Dylan através de seis personagens bastante distintos. Para isso, serão observados mais minuciosamente três personagens da película, os mais ligados ao universo musical, a saber, o menino que se apresenta como Woody Guthrie, Jack Rollins e Jude Quinn. Os outros três personagens (Robbie Clark, Arthur Rimbaud e Billy the Kid) serão discutidos à medida que suscitam temas relevantes para a compreensão da obra. Tal análise permite concluir que o tema central de Im Not There é a indústria cultural. A partir disso, é possível então ver de que maneira essa questão é abordada pelo filme e como ela serve de ponto de partida para uma discussão mais ampla, sobre o panorama social e político da década de 1960 nos Estados Unidos. Por fim, será debatida a importância de um filme contemporâneo ter como mote os anos 60 e como o próprio Im Not There, enquanto objeto de cultura, se relaciona com a discussão que ele próprio propõe sobre a indústria cultural The aim of the present work is to analyze the movie Im Not There (USA, 2007), by the American director Todd Haynes. The analysis will have as its main goal pinpointing the central theme of the movie, which portrays the life and career of Bob Dylan through six very distinct characters. Therefore, three characters will be thoroughly observed, the ones more closely connected to the musical universe, namely the boy who presents himself as Woody Guthrie, Jack Rollins and Jude Quinn. The other three characters (Robbie Clark, Arthur Rimbaud and Billy the Kid) will be discussed insofar as they raise relevant issues for understanding the film. This analysis leads to the conclusion that the main topic in Im Not There is the culture industry. It is thus possible to see in which way the movie deals with this issue and how the theme works as a starting point for a broader discussion concerning the social and political panorama of the 1960s in the United States. Finally, it is necessary to debate the importance of a contemporary movie having as its topic the 1960s and how Im Not There itself, as a cultural object, relates to the discussion it proposes regarding the culture industry

APA, Harvard, Vancouver, ISO, and other styles

20

Gao, Song [Verfasser]. "Structural and functional study of human MxA protein / Song Gao." Berlin : Freie Universität Berlin, 2011. http://d-nb.info/1025550056/34.

Full text

APA, Harvard, Vancouver, ISO, and other styles

21

Neff-Mayson, Heather. "Redemption songs : the voice of protest in the poetry of Afro-Americans /." Bern : Francke, 1989. http://catalogue.bnf.fr/ark:/12148/cb355192701.

Full text

APA, Harvard, Vancouver, ISO, and other styles

22

Nitopi, Stefano <1996&gt. "Postcolonial Songs of Protest: A Study of Bob Marley's Music and Lyrics." Master's Degree Thesis, Università Ca' Foscari Venezia, 2021. http://hdl.handle.net/10579/20014.

Full text

Abstract:

Questa tesi è incentrata sulla figura di Bob Marley in quanto autore riconducibile agli studi postcoloniali e al post-colonialismo. Bob Marley, nato Robert Nesta Marley, è stato un cantautore di origini giamaicane. Dopo aver vissuto gran parte della propria gioventù in povertà, essendo anche cresciuto solamente dalla madre, decide di dedicarsi alla musica per poter rappresentare e soprattutto criticare la condizione sociale che, come lui, molti altri giamaicani stavano vivendo. Di particolare importanza è inoltre la sua collaborazione con il gruppo “The Wailers”. Nel corso degli anni le sue canzoni diventano famose in tutto il mondo, e i suoi testi diventano rappresentazione non solo della situazione in Giamaica, ma cominciano a rivolgersi a tutta la popolazione africana o di origine africana. Nello specifico, nella sua musica possiamo ritrovare tematiche come la lotta contro l'oppressione politica e razziale e soprattutto un invito all'unificazione dei popoli di colore, dato che, a suo parere, questo era l’unico modo per poter ottenere la libertà e l'uguaglianza. La tesi si occuperà innanzitutto di contestualizzare da un punto di vista critico la tematica trattata, analizzando il significato di post-colonialismo, ed inquadrando come questo filone critico venga applicato alla letteratura. In seguito a ciò, verrà fornita un’analisi storico-sociale della Giamaica in quanto ex-colonia britannica; ciò sarà indispensabile per poter poi comprendere appieno il contesto sociale che ha portato alla nascita di Bob Marley in quanto autore. In seguito, la tesi si focalizzerà sul soggetto in questione, ovvero il cantante Bob Marley, cercando di tracciare innanzitutto una biografia, in modo da capire come i suoi testi scaturiscano dalle esperienze vissute nel corso della sua vita. Inoltre, verrà anche analizzata la sua carriera ed evoluzione nell’ambito della musica, così da poter delineare le tematiche che hanno caratterizzato le canzoni di questo autore, e poter comprendere come la produzione artistica di Bob Marley sia divenuta celebre in tutto il mondo. Infine, questa tesi si focalizzerà sull’analisi di alcuni testi selezionati, che verranno analizzati sia da un punto di visto prettamente storico-sociale, sia da un punto di vista strettamente letterario. Lo scopo di questa tesi è appunto quello di dimostrare come Bob Marley sia stato non solo un cantante di fama mondiale, ma anche un artista che ha avuto la capacità di rappresentare, attraverso la sua produzione musicale, le condizioni e le speranze nutrite sia dai Giamaicani, che da molte altre persone nel resto del mondo.

APA, Harvard, Vancouver, ISO, and other styles

23

Ipema, Tim M. "The voices of protest in Uncle Tom's Cabin and Native Son /." View online, 1990. http://repository.eiu.edu/theses/docs/32211998880394.pdf.

Full text

APA, Harvard, Vancouver, ISO, and other styles

24

Osterno, Maria do Livramento Rios. "A canÃÃo engajada nos anos 80: o rock nÃo errou." Universidade Federal do CearÃ, 2009. http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=3846.

Full text

Abstract:

Essa pesquisa se filia Ã concepÃÃo de COSTA (2001) de que o discurso literomusical enquanto prÃtica discursiva serve de referÃncia para os sujeitos, como legitimador de verdades e disseminador de maneiras de como esses sujeitos interagem com a realidade, com os outros sujeitos e com eles prÃprios. Essa nossa associaÃÃo ao pensamento de Costa(2001) recrudesce ainda mais posto que o escopo de nosso projeto foram as canÃÃes engajadas, de protesto, ou neoprotesto da comunidade discursiva do rock80, o chamado Brock, momento musical de grande inserÃÃo no meio da juventude, onde o discurso alheio dos rockeiros serviu de alteridade e em muito se confundiu com a palavra autoral dessa juventude lhe modificando idÃias, maneira de pensar, sentir e agir. A teoria de base que norteou o nosso trabalho foi a AnÃlise do Discurso de linha francesa, material teÃrico fornecido por Dominique Maingueneau(2001). Nossa pesquisa se propÃs a investigar as categorias ethos, cenografia e cÃdigo lingÃÃstico no discurso dessas canÃÃes, tendo como referencial o engajamento seminal da linha de protesto das canÃÃes da dÃcada de sessenta, escolhendo canÃÃes matrizes emblemÃticas do cancioneiro de Geraldo VandrÃ, ZÃ KÃti e SÃrgio Ricardo. Comprovamos o engajamento das canÃÃes rockeiras de 80 via materialidade lingÃÃstica expressa no cÃdigo, na captaÃÃo do ethos de seus enunciadores quando da construÃÃo da cenografia. A pesquisa esclareceu e comprovou nossa posiÃÃo inicial acerca da existÃncia das marcas de protesto nos dois gestos enunciativos: tanto na comunidade literomusical dos anos sessenta quanto na tribo rockeira de 80, aquela deixando sua marca registrada em preto e branco, de forma analÃgica, e esta pontuando no seu discurso o colorido e o formato digital. Usando uma linguagem midiÃtica visual, apenas os formatos Ã que se emprestaram subservientes ao protesto das canÃÃes. This research joins Costaâs (2001) conception that the literary and musical speech as a discursive practice serves as a reference to the subjects, legitimizing truths and spreading ways how those subjects interact with reality, with other subjects and with themselves. Our association with Costasâs mind increased further when the objective of our project was defined as the engaged songs and the songs of protest and neoprotest of the speech community of rock80, called Brock, a musical moment with great integration of youth, when the amused discourse of the rockers served as interaction and was confused with the copyright word of those young people, changing their ideas and the way they thought, felt and acted. The theory that guided our paper was the Speech Analysis by French line, the theoretic material provided by Maingueneau (2001). Our research set out to investigate the following categories ethos, set design and linguistics code in the speech of those songs, taking as reference the seminal engagement of the protest line which was in the songs of the sixties, choosing original songs of Geraldo VandrÃ, ZÃ KÃti e SÃrgio Ricardo as emblem. In this paper, we proved the involvement of rock songs of eighties observing the linguistics materiality inside the code, at the capture of its ethos by those speakers, which was reflected in the construction of the scenery. The research clarified and confirmed our initial position about the existence of marks of protest in both the gestures: the literary and musical community of the sixties and the rock tribe of eighties, as well. The first one left its trademark in an analog, black and white speech, whereas the last one left its speech in a colorful, digital format. Using a visual media language, only the formats were subservient to the protest of those songs.

APA, Harvard, Vancouver, ISO, and other styles

25

Duncan, Kelli Adams. "The Role of Ribosomal Protein L7, An Estrogen Receptor Coactivator, on the Development of Zebra Finch (Taeniopygia Guttata) Song System." Digital Archive @ GSU, 2008. http://digitalarchive.gsu.edu/biology_diss/47.

Full text

Abstract:

The Australian zebra finch (Taeniopygia guttata) serves as an excellent model organism for studying the mechanisms that influence brain sexual differentiation. The brain and behavior of the zebra finch are sexually dimorphic. The regions of the brain that control the learning and production of song (song control nuclei) are significantly larger in the male brain than in the female brain and only males sing courtship songs, thus the majority of past research has focused on the development of these sex differences. In the majority of mammals, brain sexual differentiation occurs because hormones secreted from the gonads act to initiate male or female brain development. In zebra finches, estradiol is sufficient to masculinize the male brain, however manipulations of developmental hormone exposure fail to fully reverse the sex differences in song nuclei size. Furthermore, genetic females induced to develop functional testicular tissue do not develop a completely masculinized song system and castration has no effect on development of the song system in males. The source of the increased estrogenic signal in male zebra finch brain has yet to be identified, but data suggest that other neuronal factors play a role in development of the song control nuclei. Coregulators, such as coactivators and corepressors, are proteins and RNA activators that work by enhancing or depressing transcriptional activity of the nuclear steroid receptor with which they associate. Coregulators also modulate the development of sex-specific brain morphology and behavior in rodents and birds and may help to explain the difficulties observed in altering song nuclei development via castration and gonadal hormone replacement. As an estrogen receptor-α coactivator, ribosomal protein L7 (RPL7) is able to make the brain more sensitive to estradiol by enhancing the effects of steroid receptor action. Therefore, this dissertation addressed the following questions regarding RPL7: (1) is RPL7 expression sexually dimorphic in the song nuclei of the zebra finch brain?; (2) is RPL7 protein expression regulated by steroid hormones?; and (3) does decreasing RPL7 protein expression with antisense oligonucleotides alter neuronal survival in vivo and song nuclei size and neuron number in vitro? Collectively, these studies will provide valuable information about the role of steroid receptor coactivators in development of the zebra finch song system and on the role of coactivators on sexual differentiation of the brain.

APA, Harvard, Vancouver, ISO, and other styles

26

Fuller, Denise Ann. "Creating Resistance on the Border: Coalitions and Counternarratives to S.B. 1070." The Ohio State University, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=osu1492606102229575.

Full text

APA, Harvard, Vancouver, ISO, and other styles

27

Zogli, Prince Kudjoe. "Analysis Of Function Of The Son1-Interacting Protein, Lnk2 In Arabidopsis Thaliana." ScholarWorks @ UVM, 2015. http://scholarworks.uvm.edu/graddis/494.

Full text

Abstract:

The Arabidopsis SON1 F-box protein was implicated in regulating a pathogen defense pathway, but its exact function in wild-type plants is unknown. As an F-box protein it was predicted that SON1 would assembles into a SON1-SCF ubiquitin ligase complex that recruits specific plant defense-related proteins for proteolysis. The yeast 2-hybrid assay was used to screen for potential substrates for a putative SON1-SCF ligase, leading to the identification of Arabidopsis LNK2 as a SON1-binding factor. Comprehensive protein-protein interaction analysis has shown that the binding of SON1 to LNK2 protein is specific, because closely related, full-length Arabidopsis F-box proteins do not interact with LNK2. However, when tested in isolation, some fragments derived from the paralog proteins did bind SON1, suggesting that higher order structure or inter-domain interference affects the ability of SON1 to recruit substrate. When analyzed for interaction domains, three regions of SON1 were identified that bind to LNK2 and a LNK2 binding region spans a conserved amino acid region. Phylogenetic analysis revealed that there is a paralogous gene called LNK1 in Arabidopsis, and both LNK1 and LNK2 are restricted to the plant kingdom. LNK2 and LNK1 are seen to be widely distributed in embryophyte seed and spore plants. Genetic analysis and complementation tests showed that LNK1 and LNK2 regulate flowering and photo-morphogenesis redundantly. Though lnk1 and lnk2 mutants look similar to wild-type plants, lnk1 lnk2 double mutant plants possess a long hypocotyls and flower late compared to wild-type plants. Because SON1 is implicated in plant defense, lnk mutants were assessed for susceptibility to a virulent oomycete pathogen, Hyaloperonospora arabidopsidis (Hpa). Interestingly, lnk mutants supported less disease development, suggesting a role of the wild-type LNK proteins in the enabling of pathogen colonization or in repressing host defenses. To confirm that each of the phenotypes described were a consequence of lnk1 and lnk2 mutations, wild type LNK1 and LNK2 were introduced into lnk1 lnk2 plants and examined. For most phenotypes, genetic complementation and thus restoration of a WT phenotype was observed. However, differences were uncovered in the ability of LNK genes to rescue the phenotypes, indicating specialization of function. The interaction of LNK2 with SON1 suggests that SON1-dependent ubiquitination and proteasomal degradation may control LNK2 abundance. I show by a cell free protein degradation assays that proteasome-based degradation of LNK2 as well as LNK1 is possible. Data showed that SON1 binds to ASK1 in-planta suggest the existence an SCFSON1 complex that targets LNK2 for polyubiquitination and its subsequent degradation by the proteasome. The data presented in this dissertation indicates a role for LNKs in flowering and plant defense and also suggest that proteasome-base regulation of LNK turnover may be utilized to regulate LNK protein abundance and proper maintenance of the circadian clock.

APA, Harvard, Vancouver, ISO, and other styles

28

Parissiadis, Anne. "La C4B-Binding protein : structure, biologie, intérêt de son dosage en pathologie." Université Louis Pasteur (Strasbourg) (1971-2008), 1990. http://www.theses.fr/1990STR1M060.

Full text

APA, Harvard, Vancouver, ISO, and other styles

29

Soni, Himanshu [Verfasser], and Karsten [Akademischer Betreuer] Rippe. "Deciphering Potential Role of Unfolded Protein Response in Glioblastoma / Himanshu Soni ; Betreuer: Karsten Rippe." Heidelberg : Universitätsbibliothek Heidelberg, 2019. http://d-nb.info/1196794537/34.

Full text

APA, Harvard, Vancouver, ISO, and other styles

30

Sikora, Romain. "Recyclage membranaire : rôle de la protéine MICAL-L1 et de son partenaire PACSINE3." Thesis, Sorbonne Paris Cité, 2015. http://www.theses.fr/2015USPCB179/document.

Full text

Abstract:

Le recyclage de récepteurs et de lipides vers la membrane plasmique, est un processus finement régulé, essentiel pour l’homéostasie de la membrane plasmique et pour la migration cellulaire. Il requière l’intervention des petites GTPases de la famille Rabs et leurs effecteurs. La protéine MICAL-L1, effecteur de plusieurs Rabs, comme Rab 8, 11, 13 et 35, a été impliquée dans le recyclage vers la membrane plasmique. Dans cette étude, nous avons identifié une nouvelle interaction entre MICAL-L1 et la PACSINE3, une protéine à domaine F-BAR capable de façonner les membranes intracellulaires et qui contribue à la génération d’endosomes de recyclage tubulaires. MICAL-L1 est nécessaire pour la localisation de la PACSINE3 au niveau des membranes des endosomes. La perturbation du complexe MICAL-L1/PACSINE3 affecte le recyclage du récepteur de la transferrine (TfR) vers la membrane plasmique. Le complexe MICAL-L1/PACSINE3 est associé à des longs tubules membranaires contenant la transferrine comme cargo. La dynamique de ségrégation et de détachement des cargos Tf à partir des tubules contenant MICAL-L1 et PACSINE3, suggère que ce complexe contrôle le tri/adressage des endosomes de recyclage vers la membrane plasmique. Notre travail révèle un nouveau mécanisme de régulation de la voie de recyclage vers la surface cellulaire The recycling to the plasma membrane of receptors and lipids is tightly regulated and is essential for PM homeostasis, adhesion and cell migration. It requires small GTPase Rab proteins and their effectors. The MICAL-L1 protein, an effector of several Rabs including Rab 8, 11, 13 and 35, has been shown to play an important role in the recycling. Here, we report a novel interaction between MICAL-L1 and the BAR domain containing protein PACSIN3/Syndapin3 that contributes to generate tubular recycling endosomes. MICAL-L1 is required for the localization of PACSIN3 to endosomal membranes. Importantly, disruption of MICAL-L1/PACSIN3 interaction promotes the transferrin receptor (TfR) delivery back to the plasma membrane. The MICAL-L1/PACSIN3 complex accumulates in elongated tubules that contain transferrin carriers. The dynamic of transferrin positive endosomes segregation from MICAL-L1/PACSIN3 tubules suggests that MICAL-L1/PACSIN3 complex controls TfR recycling endosomes delivery to the plasma membrane. Our data provide novel mechanistic insights on the dynamical regulation of the plasma membrane recycling pathway

APA, Harvard, Vancouver, ISO, and other styles

31

Harris, Abril N. "Deadly force| Perceptions of police and exploration of strategies used by African American mothers to protect their sons." Thesis, California State University, Long Beach, 2017. http://pqdtopen.proquest.com/#viewpdf?dispub=10263757.

Full text

Abstract:

The African American community’s relationship with the police has historically been strained for more than a century. How that tumultuous relationship affects African American mother’s perceptions of police and confidence in the ability for police to interact safely with their sons has not been explored thus far. It is the intention of this study to explore and offer insight into the experience of African American mothers with sons and potential police interactions. This qualitative study utilized a focus group setting to gather information. Within two weeks of the focus group 6 participants withdrew, the focus group ultimately included 6 participants. Participants voiced their need to educate their sons about police, Blackness being a risk factor, the emotional burdens of ensuring safety, strategies used to promote safety in potential police interactions, and possible solutions to strained community police relations. Participants developed strategies to increase safety by asking their sons to be compliant, stay under the radar, utilize family support, recreational and community resources. While there was a lack of confidence and mistrust of the police, participants maintained hope that with training and positive community interactions there can be improvement in safety during police interactions.

APA, Harvard, Vancouver, ISO, and other styles

32

Delboni, Luis Fernando. "Estrutura cristalográfica da enzima triosefosfato isomerase de Bacillus stearothermophilus e desenho racional de drogas contra a doença do sono." Universidade de São Paulo, 1995. http://www.teses.usp.br/teses/disponiveis/76/76132/tde-06122013-142443/.

Full text

Abstract:

O atual crescimento do número de estruturas tridimensionais de proteínas está produzindo um banco de dados de estruturas que é muito útil como ponto de partida para o desenvolvimento de novas moléculas relevantes do ponto de vista médico tais como drogas e vacinas. Neste trabalho, parte do projeto de desenvolvimento de novas drogas contra a doença do sono foi feita baseada nos estudos cristalográficos de ligação de compostos e de modelagem molecular de duas enzimas glicolíticas presentes no glicossomo de T. brucei. Como o alvo para novas drogas e o ciclo de glicólise, o qual também é presente nos seres humanos, o novo ligante deve ser seletivo. Frutose-1,6-bisfosfato aldolase (ALDO) é uma das proteínas alvo para o desenho racional de drogas. A análise estrutural e estudos cristalográficos da ALDO de T. brucei foram feitos, através do uso de proteína produzida em grandes quantidades por via recombinante. Vários possíveis sítios de seletividade foram encontrados em ALDO de T. brucei quando comparada as três seqüências das isoenzimas humana, tendo como base a estrutura tridimensional da isoenzima ALDO A. O braço flexível da região C-terminal é um forte alvo para compostos seletivos devido a não-conservação das seqüências. Cristais foram crescidos e padrões de difração de raios-X foram obtidos até 3.0&#197, mas ainda os cristais são pequenos e mostram-se sensíveis a radiação. Triosefosato isomerase (TIM) é outra ptoteína alvo analisada neste trabalho. A estrutura da TIM de T. brucei é conhecida a alta resolução. Cristais de proteína expressa em bactérias foram obtidos após extensivos experimentos. Foi identificado que usar proteína recentemente preparada é essencial para obter cristais que apresentem boa qualidade de difração. Depois de obtidos cristais de proteína expressa em grades quantidades, foram feitos experimentos de difusão de novos inibidores, coleta de dados de difração e análises das estruturas dos possíveis complexos. A conhecida estrutura em volta flexível da TIM fecha quando se liga o substrato ou um inibidor análogo ao substrato no sítio ativo. Desenho racional de drogas tem sido seguido usando como padrão a conformação fechada da estrutura em volta. No entanto, com a obtenção de um novo complexo, no qual a estrutura em volta adota a conformação aberta foi feita uma busca de novos compostos em bancos de dados, que não fossem derivados de fosfatos e fosfoantos, através do programa DOCK. Com este procedimento são propostos novos compostos guia os quais serão utilizados no ciclo do desenho racional de drogas. A estrutura da triosefosfato isomerase de Bacillus stearothermophilus que apresenta estabilidade térmica em complexo com o inibidor competitivo 2PG foi determinada por cristalografia de raios-X à resolução de 2.8&#197. A estrutura foi resolvida por substituição molecular usando o programa XPLOR. Promediação da densidade eletrônica de ordem dois e nivelamento do solvente foram aplicados para melhorar a qualidade do mapa. Ambos os sítios ativos estavam ocupados pelo inibidor e a estrutura emvolta flexível adota a conformação fechada em ambas as subunidades. O fator cristalográfico R é de 17,6% com boa geometria. Bacillus stearothermophillus é considerado um organismo de estabilidade térmica moderada. Encontram-se disponíveis cinco estruturas de triosefosfato isomerase de organismos mesofílicos. A estrutura obtida neste trabalho é a primeira TIM reportada que apresenta estabilidade térmica. Vários artigos têm listados fatores de estabilidade térmica, os quais foram analisados em todas as estruturas de TIM disponíveis. Neste trabalho foi conduzida uma comparação entre a estrutura termofílica e as mesofílicas disponíveis, da qual se concluiu que a interação hidrofóbica na formação do dímero, e o alto número de prolinas são os mais importantes fatores que contribuem para a estabilidade térmica da TIM de B.stearothermophilus. Também concluiu-se que a razão Arg/(arg+Lys) é elevada em TIM de B. stearothermophilus mais devido ao baixo número de lisinas do que a um alto número de argininas. Analisou-se as argininas na TIM de B. stearothermophilus como também as interações das argininas e lisinas em todas as outras estruturas de TIM e não foi possível relacionar o valor aumentado da razão Arg/(arg+Lys) com a estabilidade térmica, fator este indicado anteriormente como importante para a estabilidade térmica The current growth in the number of known 3-dimensional proteins structures is producing a database of structures that is very useful as starting point for the development of new medically relevant molecules such as drugs and vaccines. In this work part of the project of developing new drugs against the sleeping sickness has been done based on crystallographic, ligand-binding and molecular modeling studies of two glycolytic glycosomal enzymes from T. brucei. As the target for new drugs is the glycolysis pathway, which is present also in the human being, the new ligand must be selective. Fructose-1,6-bisphosphate aldolase (ALDO) is one of the target protein for the rational drug design. The structural analysis and crystallographic studies of ALDO from T. brucei have been done, from overexpressed protein. Several possible sites for selective inhibitor were found in T. brucei ALDO when compared with the three human isoenzyme sequences, based on the human ALDO A structure. The flexible C-terminal arm is a promising target for selective compounds, due to the non-conserved sequence. Crystals were grown, and X-ray diffraction was obtained up to 3.0&#197, but still the crystals are small and show radiation damage. Triosephosphate isomerase (TIM) is the other target protein analyzed in this work. The structure of TIM from T. brucei is already known at high resolution. Crystals from overexpressed protein were obtained after extensive experiments. It was identified that the use of fresh protein is essential to grow good diffracting crystals. After obtaining crystals from overexpressed protein, it has been done soaking of new inhibitors, diffraction data collection and analysis of the structures of the possible complexes. The well known flexible loop in TIM closes upon the binding of either the substrate or an inhibitor analogue to the substrate in the active site. Rational drug design has been followed based on the \"closed\" conformation of the flexible loop. Nevertheless, with a new complex structure, in which the flexible loop adopts the \"open\" conformation, it has been done a search for new compounds in the database, non-derived from phosphate and phosphonate, through the program DOCK. Based on this procedure it was proposed new lead compounds which will be used on the rational drug design cycle. The structure of the thermostable triosephosphate isomerase from Bacillus stearothermophilus in complex with the competitive inhibitor 2PG was determined by X-ray crystallography to a resolution of 2.8&#197. The structure was solved by molecular replacement using XPLOR. Two fold averaging and solvent flattening were applied to improve the quality of the map. Both of the active sites were occupied by the inhibitor and the flexible loop adopts the \"close\" conformation in both subunits. The crystallographic R-factor is 17.6% with good geometry. Bacillus stearothermophilus is considered a moderate thermophile organism. There are already five triosephosphate isomerase structures available from mesophilic organisms. The structure reported here is the first thermostable TIM reported. It has been conducted in this work a comparison between the thermophilic and the mesophilic protein structures available. Several reports had listed thermostable factors, which have been analyzed in all TIM structures available, from which it has been concluded that the hydrophobic interaction upon the dimmer formation and the higher number of proline residues are the most important factors that contribute to the thermostability of B. stearothermophilus TIM. Also it has been concluded that the ratio Arg/(Arg+Lys) is increased in B. stearothermophilus TIM more due to lower number of lysine instead of a higher number of arginine residues. Arginines have been analyze in B. s,tearothermophillis TIM as well as the interactions of arginines and lysines in all other TIM structures and it has not been possible to relate the increased ratio Arg/(Arg+Lys) with thermo stability, which was previously reported as important in thermostability

APA, Harvard, Vancouver, ISO, and other styles

33

Attarhaie, Tehrani Mahtab. "Anatomical Expression and Functional Role of the G-Protein Coupled Estrogen Receptor 1 in the Song System of Zebra Finches (Taeniopygia guttata)." Kent State University / OhioLINK, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=kent152416406994131.

Full text

APA, Harvard, Vancouver, ISO, and other styles

34

Song, Shangqing [Verfasser], and Hans-Joachim [Akademischer Betreuer] Anders. "A histology-based four protein array for postoperative outcome prediction in clear cell renal cell carcinoma / Shangqing Song ; Betreuer: Hans-Joachim Anders." München : Universitätsbibliothek der Ludwig-Maximilians-Universität, 2017. http://d-nb.info/114485718X/34.

Full text

APA, Harvard, Vancouver, ISO, and other styles

35

Laux, Katie M. "Songs in the key of protest how music reflects the social turbulence in America from the late 1950s to the early 1970s /." Oxford, Ohio : Miami University, 2007. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=miami1184767254.

Full text

APA, Harvard, Vancouver, ISO, and other styles

36

Laux, Katie. "SONGS IN THE KEY OF PROTEST: HOW MUSIC REFLECTS THE SOCIAL TURBULENCE IN AMERICA FROM THE LATE 1950S TO THE EARLY 1970S." Miami University / OhioLINK, 2007. http://rave.ohiolink.edu/etdc/view?acc_num=miami1184767254.

Full text

APA, Harvard, Vancouver, ISO, and other styles

37

Song, Fei [Verfasser], and Gerd [Akademischer Betreuer] Sutter. "Characterization of recombinant Modified Vaccinia virus Ankara for delivery of Middle East Respiratory Syndrome Coronavirus spike protein antigens / Fei Song. Betreuer: Gerd Sutter." München : Universitätsbibliothek der Ludwig-Maximilians-Universität, 2014. http://d-nb.info/105491477X/34.

Full text

APA, Harvard, Vancouver, ISO, and other styles

38

Oliveira, Renato Watanabe de [UNIFESP]. "Avaliação do efeito da hipóxia intermitente na expressão e atividade da Neprilisina (EC 3.4.24.11), uma protease envolvida na Doença de Alzheimer." Universidade Federal de São Paulo (UNIFESP), 2014. http://repositorio.unifesp.br/handle/11600/23282.

Full text

Abstract:

Made available in DSpace on 2015-12-06T23:46:45Z (GMT). No. of bitstreams: 0 Previous issue date: 2014 Associação Fundo de Incentivo à Psicofarmacologia (AFIP) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) A hipoxia e caracterizada pela reducao de oxigenio no sangue e esta relacionada a diversas patologias, entre elas a Sindrome da Apneia Obstrutiva do Sono (SAOS). Suas consequencias ainda nao estao completamente esclarecidas, mas evidencias da literatura demonstram que a hipoxia induz alteracoes bioquimicas com reflexos em aspectos comportamentais e importantes consequencias em diversos sistemas, como o sistema nervoso central, respiratorio e cardiovascular. A hipoxia e a idade apresentam uma intima correlacao com o desenvolvimento da Doenca de Alzheimer. A neprilisina (EC 3.4.24.11) e uma metaloprotease presente em diversos tecidos como rins, intestino, pulmao, cerebro, entre outros. Ja esta demonstrado que uma das principais atividades da enzima no cerebro e a degradacao do peptideo β amiloide, um dos principais responsaveis pelo surgimento e agravamento da Doenca de Alzheimer. Assim, nosso objetivo foi avaliar os efeitos da hipoxia intermitente, um modelo de SAOS, na expressao e atividade da neprilisina e na memoria e aprendizado dos animais. Metodos: Ratos machos wistar foram separados em 3 grupos: Controle, Hipoxia e Rebote. Os grupos hipoxia intermitente e rebote foram submetidos ao protocolo de hipoxia intermitente (HI-ciclos de 3 min de 21% a 5% de O2) das 8 as 16h, por seis semanas. Apos a HI, o grupo rebote foi mantido por 2 semanas em condicoes de normoxia. Realizou-se os ensaios do Labirinto aquatico de Morris (LAM), de RT-PCR, Western blot e de atividade enzimatica para neprilisina e para proteina precursora amiloide (APP) nas regioes cerebrais do hipocampo, cortex frontal e temporal. Resultados: A HI nao promoveu alteracoes na memoria e aprendizado dos animais, avaliados por LAM. Os ensaios de RT-PCR, Western blot e atividade enzimatica demonstraram um aumento da enzima seletivamente na regiao do cortex temporal. A expressao proteica da proteina precursora amiloide se manteve inalterada. Conclusoes: Apesar da HI nao afetar o desempenho dos animais no LAM, houve o aumento da expressao e atividade da enzima seletivamente no cortex temporal, uma area relacionada a cognicao, podendo levar a alteracoes ainda nao esclarecidas BV UNIFESP: Teses e dissertações

APA, Harvard, Vancouver, ISO, and other styles

39

Ronsin, Christophe. "Caracterisation d'un nouveau recepteur tyrosine-kinase et identification de son ligand, le macrophage stimulating protein." Nantes, 1994. http://www.theses.fr/1994NANT2034.

Full text

Abstract:

Par criblages successifs d'une bande d'adnc de keratinocytes humaines normaux, nous avons isole un adnc complet codant pour un nouveau membre de la famille des recepteurs tyrosine-kinases. La sequence en amino-acides de ce recepteur denomme ron (recepteur d'origine nantaise) presente une homologie significative avec le hgfr recepteur tyrosine-kinase pour le hgf. Nous avons entrepris de caracteriser biochimiquement le recepteur ron et d'identifier son ligand specifique. Nous avons montre que la proteine ron est exprimee sous deux formes: une forme dimerique (forme clivee), une forme monomerique (forme non clivee). La forme dimerique est constituee d'un domaine extra-cellulaire, un domaine trans-membranaire et un domaine intracellulaire. Nous avons emis l'hypothese qu'un facteur denomme msp (macrophage stimulating protein) presentant une homologie importante avec le hgf (ligand du hgf) correspondait au ligand specifique de ron. Notre etude a montre que l'adjonction de msp sur des keratinocytes humaines normaux entraine une augmentation du niveau de phosphorylation sur tyrosine de la forme dimerique du recepteur ron et que le domaine tyrosine-kinase est porte par la chaine lourde du recepteur. De son cote, l'equipe de edward leonard en utilisant les techniques de binding et cross-linking a confirme que le msp se fixe bien sur le recepteur ron. L'expression du transcrit ron a ete observee dans du tissu colique, et dans les keratinocytes normaux humains

APA, Harvard, Vancouver, ISO, and other styles

40

Perrot, Nahuel. "Production dans Escherichia coli de vésicules enrichies en cavéoline-1(32-178) canine ou son fragment (76-178)." Thesis, Université Paris-Saclay (ComUE), 2015. http://www.theses.fr/2015SACLS067.

Full text

Abstract:

La cavéoline-1, une petite protéine membranaire de 21 kDa, est la protéinemembranaire majoritaire d'invaginations de la membrane cytoplasmique appeléescavéoles. Enrichis en cholestérol et sphingolipides, ces domaines ont un rôleimportant dans de nombreux aspects de la vie cellulaire et constituent une véritableplateforme d'interactions protéiques et lipidiques. Ces dernières années, malgré lenombre important de travaux concluant à l'implication de la cavéoline-1, ou descavéoles, dans de nombreux processus cellulaires ou pathologiques, les donnéesquant à l'organisation de cette protéine au sein de la membrane plasmique restent trèséparses. Aussi, l'objectif principal de ce travail est de contribuer à l'acquisition dedonnées structurales sur cette protéine. Ce travail se base sur les expressionshétérologues d'une isoforme de la cavéoline-1 canineou de l'un de ses fragments, dans un hôte bactérien, sous la forme de protéines defusion associées à la Maltose Binding Protein. Ces expressions induisent laformation de vésicules intracytosoliques composées majoritairement de la protéineexprimée. Aussi, la première partie du travail est consacré à la mise en place d'unprotocole de purification de ces vésicules dans des conditions natives, répondant aucritère de réaliser une étude structurale de cette protéine n'impliquant pas l'usage dedétergent. La deuxième partie porte sur une application potentielle de ces vésicules, eten particulier pour des essais de caractérisation d'une enzyme membranaire,la glutathion-S-transférase microsomale de rat. Enfin une troisième partie est dédiée àl'analyse de simulations de dynamique moléculaire dans le cadre d'étudesd'interactions au sein de systèmes membranaires Caveolin-1, a 21 kDa membrane protein, is the principal membrane protein ofcytoplasmic membrane domains named caveolae. Specifically enriched incholesterol and sphingolipids those domains are important in many aspect of the cell's life and make up a proteins and lipids interaction platform. In the past years, despite a large number of publications stating the implication of caveolin-1 or caveolae in many cell processes and pathologies, very few is known about theway this protein is organized at the cell membrane. Hence, the main purpose of thiswork is to contribute to the acquisition of structural data on this protein. At the base of this work is the heterologous expression within a bacterial host, and as a fusion protein, of the canin beta isoform of cavéolin-1 or one of its fragment. These expressions lead to the formation of cytoplasmic vesicles composed mainly ofthe expressed protein. Thence, the first part of this work focus on developping a method to purify those vesicles that does not rely on using any kind of detergent which could enable structural studies in a native environnment. The second part present a potentiel application of those vesicles and inparticular the use of those vesicles to characterize a membrane enzyme, namelythe murin microsomal glutathion-Stransferase. The last part will be a contribution to data analysis within the context of molecular dynamics simulationof membraneous systems

APA, Harvard, Vancouver, ISO, and other styles

41

Dosnon, Marion. "Déterminants moléculaires de l'affinité de l'intéraction entre la protéine désordonnée NTAIL et son partenaire XD chez le virus de la rougeole." Thesis, Aix-Marseille, 2015. http://www.theses.fr/2015AIXM4080.

Full text

Abstract:

Les IDPs sont des protéines dépourvues de structure 3D unique en solution et en l'absence de leur(s) partenaire(s). Ces protéines possèdent des propriétés d'interactions avec leurs partenaires uniques.L'extrêmité C-terminale de la nucléoprotéine du virus de la rougeole, NTAIL, est une IDP. NTAIL interagit avec XD, le domaine C-terminal globulaire de la phosphoprotéine virale, via la box2 qui est un a-MoRE. Cette interaction permet le recrutement de la protéine L afin de former la polymérase virale.J'ai pu montrer que la contribution des différents résidus au sein de l'a-MoRE dépend de l'orientation de leur chaîne latérale, et que la substitution d'un seul acide aminé crucial a des effets dramatiques sur la réplication virale.Les IDPs conservent un désordre résiduel non négligeable au sein du complexe. Cela se manifeste par la présence des régions « fuzzy » de part et d'autres du MoRE. Nous avons montré que la région « fuzzy » en amont de la box2 inhibe l'établissement de l'interaction entre NTAIL et ses partenaires.Lors de l'interaction avec leurs partenaires les IDPs subissent en général un gain de structure. Le repliement associé à l'interaction peut avoir lieu avant ou après interaction. Des études précédentes ont montré l'existence d'une pré-structuration partielle de l'alpha-MoRE de NTAIL. L'interaction entre NTAIL et XD a été étudiée et a permis de conclure que NTAIL se replie selon un mécanisme de repliement induit.Dans leur ensemble, ces études contribuent à éclaircir les mécanismes moléculaires qui gouvernent la reconnaissance de partenaires par les IDPs IDPs are proteins devoided of a unique and stable 3D structure in solution and in the absence of their partners. Those proteins possess unique properties, as well as mechanisms of interaction with their partners.The C-terminal region of the nucleoprotein of measles virus, NTAIL, is an IDP and interacts with XD, the globular C-terminal domain of the viral phosphoprotein, via the box2 region that is an (alpha-MoRE). This interaction allows to recruit the L protein in order to form the viral polymerase.The aim of my work was to characterize the molecular basis of NTAIL-XD interaction. I was able to show that the contribution of the amino acids among box2 depends on the orientation of their lateral chain, and that the substitution of one single amino acid has drastic effect upon the viral replication.IDPs keep a non-negligible amount of residual disorder among the complex. This fuzziness can have multiple forms, like the presence of fuzzy regions from either side of the MoRE. The impact fuzzy regions have within the complex is not well known. We demonstrated that the fuzzy region upstream box2 inhibits the settlement of the interaction between NTAIL and its partners.When interacting with their partners, IDPs generally undergo a folding associated with binding that can take place either before or after the interaction. The interaction between NTAIL and XD was investigated and monitored by fluorescence kinetic measurements, using variants bearing a tryptophan substitution. We concluded without any doubt that NTAIL folds under an induced folding mechanism.Those studies together contribute to enlighten the molecular mechanisms that govern partner recognition by the IDPs

APA, Harvard, Vancouver, ISO, and other styles

42

Pierre, Virginie. "L'acrosome du spermatozoïde de sa biogenèse à son rôle physiologique." Thesis, Grenoble, 2013. http://www.theses.fr/2013GRENS035/document.

Full text

Abstract:

Le spermatozoïde est une cellule hautement spécialisée qui doit être capable de réaliser des fonctionsspécifiques pour être capable de féconder un ovocyte. Il doit être capable de réaliser une réactionacrosomique qui consiste en l’exocytose d’une vésicule géante de sécrétion attachée au noyau. Cettevésicule contient des enzymes qui vont permettre au spermatozoïde de traverser la zone pellucide quientoure l’ovocyte. Mon travail a consisté à étudier l’effet d’une des enzymes contenue dansl’acrosome, la sPLA2 de mammifère de groupe X (mGX). C’est la seule phospholipase demammifères parmi les 5 testées qui a un effet d’inhibition sur une population spécifique despermatozoïdes ayant une mobilité diminuée. Mon travail a ainsi confirmé la spécificité de cettephospolipase sur la régulation de la physiologie spermatique. Dans un deuxième temps, j’ai participéà la découverte du gène DPY19L2 impliqué dans une infertilité masculine rare, la globozoospermie.La globozoospermie se caractérise par des spermatozoïdes ayant une tête ronde dépourvued’acrosome. Le gène DPY19L2 est spécifiquement exprimé dans les testicules, il est absent chez80% des patients globozoospermiques. J’ai caractérisé le rôle de cette protéine et montré qu’elle estimpliquée dans l’attachement de l’acrosome au noyau. J’ai pu montrer que cette protéine appartient àla membrane nucléaire interne où elle interagit avec la protéine Sun5, une protéine qui appartientaussi à la membrane nucléaire interne et dont l’expression est spécifique à la spermiogénèse. Sun5est impliquée dans la formation de complexes LINC (Linker of Nucleoskeleton and Cytoskeleton)qui permettent de relier le cytosquelette au nucléosquelette, constitué entre autres par les lamines. Lerôle de DPY19L2 pourrait permettre de stabiliser l’ancrage de la protéine SUN5 afin de transmettreles forces exercées par le cytosquelette au noyau de la spermatide. DPY19L2 appartient à une famillede protéines DPY19L1 à L4 dont les fonctions restent encore peu caractérisées. Une étude récentemontre qu’une diminution de l’expression de Dpy19l1 chez la souris entraîne un défaut de migrationdes neurones glutamatergiques sur la glie radiale. Mon travail a montré l’importance de DPY19L2dans le contrôle des interactions noyau-cytosquelette et devrait permettre de mieux comprendre lerôle des autres protéines de cette famille dans divers organes The spermatozoon is a highly specialized cell that must be able to perform specific functions tofertilize the oocyte. It must be able to perform the acrosome reaction, an exocytosis of a giant vesicleof secretion, attached to the nucleus. This vesicle contains enzymes that allow the sperm to penetratethe zona pellucida surrounding the oocyte. The aim of my work was first to study the effect of anenzyme present in the acrosome, the sPLA2 of group X in mouse (mGX). This is the onlymammalian phospholipase among the five tested that has an inhibitory effect on sperm specificpopulation with low mobility. My work has confirmed the specificity of this phospolipase on theregulation of sperm physiology. Second, I participated in the discovery of the gene DPY19L2involved in male infertility, globozoospermia. The globozoospermia is characterized by round headspermatozoa without acrosome. DPY19L2 gene is specifically expressed in the testis and is absent in80% of globozoospermic patients. I then identified the role of this protein, which is involved in theattachment of the acrosome to the nucleus. I showed that this protein belongs to the inner nuclearmembrane where it likely interacts with the protein sun5 which also belongs to the inner nuclearmembrane and whose expression is specific to spermiogenesis. Sun5 is involved in the complexformation, called LINC that connects the cytoskeleton to the nucleoskeleton lamina. The role ofDpy19l2 could help stabilizing the anchoring of protein sun5 in order to transmit the forces exertedby the cytoskeleton to the nucleus of the spermatid during acrosome spreading. Dpy19l2 belongs to aprotein family containing 4 members, Dpy19l1 to l4, which has not been poorly studied so far. Arecent study shows that the knock-down of Dpy19l1 resulted in defective glutamatergics neuronsmigration on the radial glia. The results obtained during my work would improve the knowledge ofCytoskeleton-nucleoskeleton interaction, and give new insight on this new family of proteins

APA, Harvard, Vancouver, ISO, and other styles

43

Osterno, Maria do Livramento Rios. "A canção engajada nos anos 80: o rock não errou." www.biblioteca.ufc.br, 2009. http://www.repositorio.ufc.br/handle/riufc/6107.

Full text

Abstract:

OSTERNO, Maria do Livramento Rios, A canção engajada nos anos 80: o rock não errou. 2009. Dissertação (Mestrado em Linguistica) – Universidade Federal do Ceará, Departamento de Letras Vernáculas, Programa de Pós-Graduação em Linguística, Fortaleza-CE, 2009. Submitted by Liliane oliveira (morena.liliane@hotmail.com) on 2012-08-28T16:40:02Z No. of bitstreams: 1 2009_DIS_MLROSTERNO.pdf: 926128 bytes, checksum: ca6d61890a19d1fde27560699281dd36 (MD5) Approved for entry into archive by Maria Josineide Góis(josineide@ufc.br) on 2013-10-10T14:00:03Z (GMT) No. of bitstreams: 1 2009_DIS_MLROSTERNO.pdf: 926128 bytes, checksum: ca6d61890a19d1fde27560699281dd36 (MD5) Made available in DSpace on 2013-10-10T14:00:03Z (GMT). No. of bitstreams: 1 2009_DIS_MLROSTERNO.pdf: 926128 bytes, checksum: ca6d61890a19d1fde27560699281dd36 (MD5) Previous issue date: 2009 This research joins Costa’s (2001) conception that the literary and musical speech as a discursive practice serves as a reference to the subjects, legitimizing truths and spreading ways how those subjects interact with reality, with other subjects and with themselves. Our association with Costas’s mind increased further when the objective of our project was defined as the engaged songs and the songs of protest and neoprotest of the speech community of rock80, called Brock, a musical moment with great integration of youth, when the amused discourse of the rockers served as interaction and was confused with the copyright word of those young people, changing their ideas and the way they thought, felt and acted. The theory that guided our paper was the Speech Analysis by French line, the theoretic material provided by Maingueneau (2001). Our research set out to investigate the following categories ethos, set design and linguistics code in the speech of those songs, taking as reference the seminal engagement of the protest line which was in the songs of the sixties, choosing original songs of Geraldo Vandré, Zé Kéti e Sérgio Ricardo as emblem. In this paper, we proved the involvement of rock songs of eighties observing the linguistics materiality inside the code, at the capture of its ethos by those speakers, which was reflected in the construction of the scenery. The research clarified and confirmed our initial position about the existence of marks of protest in both the gestures: the literary and musical community of the sixties and the rock tribe of eighties, as well. The first one left its trademark in an analog, black and white speech, whereas the last one left its speech in a colorful, digital format. Using a visual media language, only the formats were subservient to the protest of those songs Essa pesquisa se filia à concepção de COSTA (2001) de que o discurso literomusical enquanto prática discursiva serve de referência para os sujeitos, como legitimador de verdades e disseminador de maneiras de como esses sujeitos interagem com a realidade, com os outros sujeitos e com eles próprios. Essa nossa associação ao pensamento de Costa(2001) recrudesce ainda mais posto que o escopo de nosso projeto foram as canções engajadas, de protesto, ou neoprotesto da comunidade discursiva do rock80, o chamado Brock, momento musical de grande inserção no meio da juventude, onde o discurso alheio dos rockeiros serviu de alteridade e em muito se confundiu com a palavra autoral dessa juventude lhe modificando idéias, maneira de pensar, sentir e agir. A teoria de base que norteou o nosso trabalho foi a Análise do Discurso de linha francesa, material teórico fornecido por Dominique Maingueneau(2001). Nossa pesquisa se propôs a investigar as categorias ethos, cenografia e código lingüístico no discurso dessas canções, tendo como referencial o engajamento seminal da linha de protesto das canções da década de sessenta, escolhendo canções matrizes emblemáticas do cancioneiro de Geraldo Vandré, Zé Kéti e Sérgio Ricardo. Comprovamos o engajamento das canções rockeiras de 80 via materialidade lingüística expressa no código, na captação do ethos de seus enunciadores quando da construção da cenografia. A pesquisa esclareceu e comprovou nossa posição inicial acerca da existência das marcas de protesto nos dois gestos enunciativos: tanto na comunidade literomusical dos anos sessenta quanto na tribo rockeira de 80, aquela deixando sua marca registrada em preto e branco, de forma analógica, e esta pontuando no seu discurso o colorido e o formato digital. Usando uma linguagem midiática visual, apenas os formatos é que se emprestaram subservientes ao protesto das canções

APA, Harvard, Vancouver, ISO, and other styles

44

FERRERO, VACHER CORINNE. "La sp 220k nouvelle protease matricielle : etude de son expression dans l'adenocarcinome renal et dans certaines tumeurs benignes renales." Nice, 1994. http://www.theses.fr/1994NICE6569.

Full text

APA, Harvard, Vancouver, ISO, and other styles

45

Rouhana, Jad. "Etude et modulation des interactions protéine-protéine : l’activation de la petite protéine G Arf1 par son facteur d’échange Arno." Thesis, Montpellier 1, 2013. http://www.theses.fr/2013MON13507/document.

Full text

Abstract:

Arf1 est une petite protéine G (pG), essentiellement impliquée dans le trafic vésiculaire. Arf1 oscille entre deux conformations, l'une active liée au GTP et l'autre inactive associée au GDP. Arno est un des facteurs d'échange (GEF) capable d'activer Arf1 en stimulant l'échange GDP/GTP. Suractivée dans les cellules invasives du cancer du sein, Arf1 joue un rôle important dans la migration et la prolifération des cellules cancéreuses.Le but de ma thèse s'inscrit dans l'étude et la modulation de l'interaction pG-GEF, et plus spécifiquement, le couple Arf1-Arno. Mon travail a été planifié autour de deux axes: (1) L'étude fine de l'interaction entre Arf1 et Arno, et sa modulation avec un inhibiteur connu la Bréféldine A (BFA). (2) La mise en place d'une stratégie de conception d'inhibiteurs de l'interaction protéine-protéine du couple Arf1-Arno.Dans un premier temps, nous avons mis en place une méthode basée sur la résonance plasmonique de surface (SPR) permettant la détermination des paramètres cinétiques de l'interaction entre Arf1 et Arno. Nous avons précisé aussi les conséquences des partenaires allostériques (GDP, GTP, et Mg2+) et de la BFA sur les paramètres cinétiques de l'interaction. Ceci a permis une analyse fine de la régulation allostérique et du mode d'action de la BFA. Appliquée à d'autres inhibiteurs, cette méthode permettra d'examiner leur mécanisme d'inhibition.Dans la deuxième partie j'expose, la stratégie que nous avons utilisé pour la conception rationnelle d'inhibiteur de l'interaction entre Arf1 et Arno. Elle est basée sur le criblage virtuel de fragments au niveau des résidus clé « hotspots » de l'interaction, la validation des molécules-touches par des techniques biophysiques, et l'élimination de molécules artefacts. Les structures des complexes fragments-Arno ont été résolues, ce qui confirme la validité de cette stratégie ouvrant la voie vers l'optimisation moléculaire pour obtenir des inhibiteurs plus efficaces Arf1 is a small GTPases, essentially involved in the vesicular traffic. Arf1 switch between two conformations, an active form bound to GTP and an inactive form bound to GDP. Arno is one of the exchange factors (GEF) that can activate Arf1, through its catalytic Sec7 domain, promoting the exchange of GDP by GTP. Activated in breast cancer cells, Arf1 plays an important role in the migration and proliferation of cancer cells.The aim of my thesis was the study and the modulation of the interaction between small G proteins and their GEFs, more precisely the Arf1-Arno interaction. My work has been planned around two axes: (1) the study of the interaction between Arf1 and Arno, and its modulation with a known inhibitor Brefeldin A (BFA). (2) The development of a rational strategy for designing inhibitors of protein-protein interaction for the Arf1-Arno complex.In the first part of my PhD work, we set up a Surface Plasmon Resonance (SPR) method allowing to determine the kinetic parameters of the interaction between Arf1 and Arno. We also studied the effects of allosteric partners such as GDP, GTP and Mg2+ as well as the known uncompetitive inhibitor (Brefeldin A). This SPR approach allowed a very informative analysis at qualitative and quantitative levels of the various complexes taking place during the exchange reaction that should help to solve the inhibitory mechanism for the known inhibitors reported in the literature. In the second part of my thesis, we propose a strategy for targeting the interaction between Arf1and Arno. This approach is based on virtual screening of fragments at hotspot regions. Using biophysical techniques such fluorescence techniques, SPR, NMR and X-Ray crystallography, we identified and validated Hits, showing by crystallographic structural data their modes of interaction with the target protein Arno. A fluorescence polarization test was also developed to identify false positive fragments to eliminate promiscuous aggregators. Taken together, our work proposes a method based on SPR allowing the study of known inhibitors of GEFs, understanding at molecular level their mode of action. We also propose a general strategy for finding Hit fragments that designing competitive inhibitor of the interaction small G protein with its GEFs, that can be the scaffold for designing more powerful inhibitors

APA, Harvard, Vancouver, ISO, and other styles

46

Paik, Su-Jin. "Couplages entre un transporteur membranaire de type ABC, BmrA et son environnement membranaire." Electronic Thesis or Diss., Paris Sciences et Lettres (ComUE), 2018. http://www.theses.fr/2018PSLET010.

Full text

Abstract:

Les ABC (ATP binding cassettes) transporteurs constituent une grande famille de protéine transmembranaire présentent dans tous les organismes. Les ABC hydrolysent l'ATP pour transporter une quantité immense de substrats amphiphiles, comme les lipides, steroids, peptides... Certains ABCs confèrent un phénotype cellulaire de multiresistance aux drogues des bactéries contre les antibiotiques à l’homme contre les agents anticancéreux, antiviraux ...Une question fondamentale pour comprendre le transport de drogues est comment les propriétés de membranes modulent la fonction et l’organisation spatio temporelle des ABCs transporteurs. Nous avons étudié en détails ces couplage avec BmrA, un ABC bactérien de B. subtillis en utilisant différents systèmes membranaires in vitro et différentes approches de biochimie et de biophysique membranaire. Dans un premier temps, après expression et purification des protéines en détergent, nous avons caractérisé l’hydrolyse d’ATP en fonction de l’état de l’environnement membranaire, solubilisé en micelle de détergent et en micelles mixtes. Les proteoliposomes ont été caractérisés en fonction de l’orientation des protéines, leur taux d’incorporation, leur taille et la lamellarité. Cela nous a permis de moduler de façon contrôlée la composition lipidique, la densité et la conformation des protéines et la courbure membranaire pour déterminer de façon quantitative les contributions respectives de ces paramètres de membranes. Ainsi, nous montrons que l’hydrolyse d’ATP est sensible à la spécificité lipidique quand la protéine est dans une bicouche et que les lipides négatifs et les lipides de type phosphatidylethanolamine stimulent de façon synergique l’activité hydrolytique. L’hydrolyse d’ATP diminue pour les fortes courbure positives. Dans un second temps, nous avons déterminé les conditions de reconstitution de BmrA dans des vésicules géantes qui ont ensuite permis d’étudier les rôles respectifs de la courbure et de la tensions membranaire dans l’organisation spatiale de BmrA par des approches de nanotube pulling. Les expériences en collaboration montrent que BmrA a une préférence marquée pour les régions membranaires à forte courbure conduisant à la formation de cluster de protéines et que cette préférence varie en fonction de l’état catalytique de la protéine. Finalement, nous avons mis au point une méthode pour étudier la dynamique des NBDs par transfert d'énergie de résonance de Förster au niveau de la molécule unique dans un système reconstitué par spectroscopie de fluorescence et de corrélation croisée.L’ensemble des données suggère que organisations spatiales des ABC transporteurs dans les cellules bactériennes et eucaryotes sont différentes avec la possibilité de tris dans des zones de fortes courbures lors de remodelages membranaires mais sans modification importante de la fonction ABCs (ATP binding cassettes) transporters constitute a large family of transmembrane proteins present in all organisms. ABC transporters hydrolyze ATP to translocate an immense quantity of amphiphilic substrates, such as lipids, steroids, peptides... Some ABCs confer a multiresistance cellular phenotype to drugs from bacteria against antibiotics to humans against anticancer agents, antivirals...A fundamental question for understanding drug transport at the molecular level is how the properties of membranes modulate the function and spatial temporal organization of ABCs. We studied in detail these coupling with BmrA, a bacterial ABC of B. subtillis using different in vitro membrane systems and different biochemical and membrane biophysical approaches. Firstly, after expression and purification of proteins in detergent, we characterized the hydrolysis of ATP of BmrA according to its membrane environment, solubilized in detergent micelles and in mixed lipid/detergent micelles. Proteoliposomes were characterized according to protein orientation, incorporation rate, size and lamellarity. This allowed us to modulate in a controlled manner lipid composition, protein density and conformation and membrane curvature to quantitatively determine the respective contributions of these membrane parameters. Thus, we show that ATP hydrolysis is sensitive to lipid specificity when the protein is embedded in a bilayer. This lipid specificity is provided by negative lipids and phosphatidylethanolamine type lipids that synergistically stimulate hydrolytic activity. However, ATP hydrolysis was decreased in high positive membrane curvature. Secondly, we determined the conditions of reconstitution of BmrA in Giant Unilamellar Vesicles, which then allowed our collaborator to study the respective roles of membrane curvature and tension in the spatial organization of BmrA. Nanotube pulling experiments performed in collaboration show that BmrA has a strong preference for highly curved membrane regions leading to protein cluster formation and that this preference varies according to the catalytic state of the protein. Finally, we developed a method to study the dynamics of NBDs by Förster resonance energy transfer at the single molecule level in reconstituted system via fluorescence cross-correlation spectroscopy.The data set suggest that spatial organizations of ABC transporters in bacterial and eukaryotic cells are different with the possibility of sorting during membrane remodeling of eukaryotic membranes in areas of strong membrane curvatures but without significant change in function

APA, Harvard, Vancouver, ISO, and other styles

47

McCollam-Guilani, Linda Sue. "Analysis of the Mechanism of Ras Activation: Mapping of Important Functional Domains of the Son of Sevenless Protein." eScholarship@UMMS, 1998. https://escholarship.umassmed.edu/gsbs_diss/90.

Full text

Abstract:

The questions outlined in this thesis dissertation were proposed in order to provide insight regarding the mechanism by which the Drosophila Son of sevenless (dSOS) protein activates Ras. Ras proteins are GTP-binding proteins which bind guanine nucleotides very tightly and cycle between the inactive GDP-bound state and the active GTP-bound state. To address the mechanism by which the dSOS proteins activates Ras, a structure-function analysis of the dSOS protein was performed using truncation and deletion mutants of dSOS. In vivo Ras activation experiments using transiently transfected cells revealed that the NH2-terminal domain of dSOS is required in order for the catalytic domain of dSOS to exhibit exchange activity in cultured mammalian cells. The COOH-terminal GRB2 (Growth Factor Receptor Binding Protein) binding domain on the otherhand was insufficient to confer Ras exchange activity to the dSOS catalytic domain. Further analysis of the NH2-terminal domain of the dSOS protein demonstrated that the function of promoting catalytic domain activity could be localized by mutational analysis to the pleckstrin (PH) and DBL (Diffuse B-cell Lymphoma) homology sequences. Fractionation studies of cells transiently transfected with various dSOS mutant proteins demonstrated that the NH2-terminus of dSOS is also necessary for membrane association. These findings suggested that the model proposing that the recruitment of SOS via the adaptor protein GRB2 to the membrane is the main mechanism by which SOS activates Ras is unlikely to be the only mechanism by which SOS can activate Ras. From our data, a model can be proposed which postulates that SOS can activate Ras as a consequence of at least two steps. One step involves the SOS/GRB2 interaction and the second step involves the NH2-terminal domain of SOS associating with unidentified cellular elements.

APA, Harvard, Vancouver, ISO, and other styles

48

Bonnard, Damien. "Activités multiples des inhibiteurs allostériques de l’interaction entre l’Intégrase du VIH-1 et son cofacteur LEDGF/p75." Thesis, Sorbonne Paris Cité, 2017. http://www.theses.fr/2017USPCC282.

Full text

Abstract:

VIH-1, l’agent étiologique du Syndrome de l’Immunodéficience Acquise, est un rétrovirus qui infecte les cellules immunitaires et détourne leur machinerie cellulaire pour se répliquer rapidement. Lors de l’infection, le génome ARN est rétrotranscrit en ADN par la transcriptase inverse virale (RT), puis l’insertion du génome proviral dans l’ADN de la cellule hôte est une étape obligatoire du cycle viral catalysée par l’enzyme virale Intégrase (IN). L’interaction de l’IN avec son cofacteur essentiel, la protéine nucléaire LEDGF/p75, dirige l’intégration à l’intérieur de gènes dans des régions fortement exprimées de la chromatine, ce qui permet la production efficace de nouveaux virions. Les Inhibiteurs Allostériques Intégrase-LEDGF (INLAIs) sont une nouvelle classe de molécules antirétrovirales se liant à l’IN au site de liaison de LEDGF/p75. Conçus pour inhiber compétitivement l’interaction protéine-protéine IN-LEDGF/p75, ils inhibent également les activités enzymatiques de l’Intégrase et augmentent son niveau de multimérisation.Nous avons étudié plusieurs nouvelles séries d’INLAIs de la société Mutabilis, et avons pu démontrer que ces molécules inhibent l’intégration, mais ont aussi un effet antirétroviral plus puissant et indépendant de LEDGF/p75 post-intégration au cours de la maturation des virions, qui conduit à la production de virus non infectieux, ayant une morphologie excentrique caractérisée par un défaut d’encapsidation du génome viral. Lors de l’infection de cellules par ces virus, le cycle viral s’arrête à l’étape de rétrotranscription du génome viral. Nous avons montré que ces virions contiennent pourtant un génome viral stable et fonctionnel, une RT active et l’ARNtLys3 qui sert d’amorce à la rétrotranscription, et ont également conservé leur immunoréactivité pour les lymphocytes B et T. En évaluant l’impact du polymorphisme de l’IN au voisinage du site de liaison, nous avons identifié le variant polymorphe Ala125, pour lequel l’INLAI MUT-A perd concomitamment son effet sur la maturation des virions et sur la multimérisation de l’IN, tandis qu’il inhibe aussi bien l’intégration et l’interaction IN-LEDGF, prouvant que l’effet tardif des INLAIs est associé à l’induction de la multimérisation de l’IN. Nous avons pu associer la multimérisation de l’IN à une déstabilisation du dimère par les INLAIs en analysant les co-structures de MUT-A avec les intégrases polymorphes. Les INLAIs, outre leur intérêt thérapeutique sont de remarquables réactifs qui ont permis de démontrer le rôle essentiel de l’intégrase à trois étapes clés du cycle viral du VIH-1 : la rétrotranscription, l’intégration et la maturation des virions HIV-1, the causative agent of AIDS, is a retrovirus that infects immune cells and hijacks their cell machinery to achieve rapid replication. In the course of infection, the RNA genome is reverse transcribed into DNA by the viral Reverse Transcriptase (RT) before the obligatory insertion of the proviral genome into the host cell DNA catalyzed by the viral enzyme Integrase (IN). The interaction of IN with its essential cofactor, the nuclear protein LEDGF/p75, targets integration within gene introns in highly transcribed chromatine regions, which allows efficient production of new virions. IN-LEDGF Allosteric Inhibitors (INLAIs) are a novel class of antiretroviral molecules binding IN at the LEDGF/p75-binding site. Designed to competitively inhibit IN-LEDGF/p75 protein-protein interaction, they are also capable of inhibiting IN enzymatic activities and raising the IN multimerization level.We studied several new INLAI series from the company Mutabilis. We could demonstrate that these molecules inhibit integration, but also have a more potent, LEDGF-independent, antiretroviral effect during virion maturation, resulting in the production of non-infectious virions. Virions produced upon INLAI treatment have an eccentric morphology characterized by an encapsidation defect of the viral genome, and lead to an infection block at reverse transcription. Yet, we showed that these virions package a stable and functional viral genome, an active RT and the tRNALys3 primer for reverse transcription, and also keep their immunoreactivity towards B- and T-cell lymphocytes. When evaluating the influence of polymorphism at the edge of the binding site, we identified the IN Ala125 polymorphic variant which causes the concomitant loss of MUT-A effect on virion maturation and IN multimerization, whereas inhibition of integration and IN-LEDGF interaction are maintained. This proves that INLAIs exert their late stage effect through induction of IN multimerization. We could associate IN multimerization to INLAI-induced dimer destabilization by analyzing MUT-A co-structures with polymorphic integrases. Beside their therapeutic interest INLAIs are highly valuable reagents that allowed to demonstrate the essential role of integrase at three key steps of the HIV-1 replication cycle, reverse transcription, integration and virus maturation

APA, Harvard, Vancouver, ISO, and other styles

49

YE, FEI. "Etude de 100 kda ras gtpase-activating protein (rasgap) : voie de transduction du signal de regulation de son expression et mise en evidence d'une nouvelle rasgap-associated protein." Paris 6, 1999. http://www.theses.fr/1999PA066531.

Full text

Abstract:

Dans ce travail de these, nous avons etudie la voie de transduction du signal impliquee dans l'expression de p100 rasgap induite par l'acide okadaique (oa) ou la staurosporine (sts), dans les choriocarcinomes humains. Nous avons montre que oa induit l'expression de p100 rasgap, active la voie de map kinase, et induit l'expression de c-fos et c-jun. De plus, c-jun/ap-1 est indispensable a l'expression de p100 rasgap. Oa agit, vraisemblablement par inhibition des phosphatases, independamment de la proteine kinase c (pkc), et fonctionne synergiquement avec l'activateur de pkc, pma. C'est la premiere etude sur les voies de signalisation de l'expression de la rasgap. De la meme approche, nous avons montre que sts, un inhibiteur des proteine kinases, induit egalement l'expression de p100 rasgap, active la voie de map kinase et induit l'expression de c-fos et c-jun. De nouveau, nous avons montre que c-jun/ap-1 est indispensable a l'expression de p100 rasgap. Contrairement a l'action de oa, sts agit par l'intermediaire de pkc malgre son large spectre d'actions comme inhibiteurs de proteine kinases. Nous avons de plus montre que la forskoline, activateur de pka, active egalement la voie de map kinase et induit l'expression de p100 rasgap sans passer par pkc. L'ensemble de resultats tente a montrer que l'expression de p100 rasgap est controlee par un reseau croise de signalisation en amont de la map kinase, passant, contournant ou combinant pkc, et regulee en aval par l'activation de la map kinase et de l'ap-1. Un autre resultat original est l'identification d'une nouvelle proteine qui est capable de s'associer a la fois p120 et p100 rasgap. Cette nouvelle rasgap-associated protein, p105, est la seule connue de pouvoir former un complexe avec p100 rasgap. Un autre aspect original de ce travail reside dans le fait que p105 existe dans les trophoblastes differencies et les choriocarcinomes induits a differencier. Par contre p105 est absente dans les choriocarcinomes. Ceci lui a conduit a proposer que p105 puisse etre un marqueur de differenciation des trophoblastes humains. Notre etude comparative de l'expression de p100 rasgap au cours de developpement in vivo et in vitro des cytotrophoblastes, ainsi que dans les choriocarcinomes induits a se differencier, a justifie la proposition de considerer p100 rasgap comme marqueur biochimique de la differenciation trophoblastique. C'est un marqueur precieux pour des etudes in vitro des trophoblastes et de leur pathologie. L'etude par la regulation de l'expression de rasgap a ete jusqu'alors negligee malgre l'important de ce modulateur cellulaire. Notre travail constitue une contribution a la connaissance de l'expression le p100 rasgap et de ses possibles fonctions associee a la differenciation cellulaire.

APA, Harvard, Vancouver, ISO, and other styles

50

Aymonnier, Karen. "La protease Nexine-1, une cible prometteuse dans le traitement de l'hémophilie et son rôle dans les polynucléaires neutrophiles." Thesis, Sorbonne Paris Cité, 2019. http://www.theses.fr/2019USPCC049.

Full text

Abstract:

L’hémophilie A est une maladie hémorragique rare caractérisée par un déficit du facteur de la coagulation VIII (FVIII). De nouvelles stratégies thérapeutiques ne reposant pas sur l’injection de FVIII se développent. Nous proposons une approche innovante qui consiste à cibler un anticoagulant naturel présent dans les plaquettes, la Protéase Nexine-1 (PN-1). La PN-1 est un inhibiteur puissant de la thrombine, enzyme clé de la coagulation. Bloquer la PN-1 favoriserait ainsi la génération de thrombine et donc la coagulation chez les patients hémophiles. Nous avons montré que bloquer la PN-1 améliore effectivement la génération de thrombine dans les plasmas de patients hémophiles. Nos données obtenues in vivo ont également montré une restauration de la coagulation et des temps de saignement chez des souris hémophiles déficientes en PN-1. L’ensemble de nos résultats indique que la PN-1 constitue une nouvelle cible thérapeutique intéressante pour le traitement de l’hémophilie.Le rôle potentiel de la PN-1 dans la régulation des mécanismes inflammatoires n’est pas connu. Au niveau des cellules inflammatoires, sa présence n’a été démontrée que dans les monocytes-macrophages et son expression n’a encore jamais été étudiée dans les polynucléaires neutrophiles. Nous avons ainsi mis en évidence pour la première fois la présence de PN-1 dans les neutrophiles et avons montré que le déficit en PN-1 limite le recrutement des neutrophiles in vivo. Ces travaux ont permis d’identifier la PN-1 comme un nouveau régulateur des mécanismes de recrutement des neutrophiles Hemophilia is a disease caused by the lack of Factor VIII (FVIII), leading to insufficient thrombin production, and therefore bleeding. Recently, new therapeutic strategies for hemophilia treatment, that do not rely on clotting factor replacement, have emerged. We propose an innovative approach consisting in targeting a natural and potent thrombin inhibitor, expressed by platelets and called protease nexin-1 (PN-1). Our results demonstrated that blocking PN-1 increased, in vitro, thrombin generation in plasma from patients with hemophilia, and reduced blood loss in hemophiliac mice. Our study provides proof-of-concept that PN-1 neutralizing can be a a novel approach for future clinical care in hemophilia. The potential role of PN-1 in regulating inflammatory processes is not known. Vascular cells, platelets and inflammatory cells express PN-1, but no data are available concerning its expression and potential function in neutrophils. For the first time, we demonstrated the presence of PN-1 in neutrophils. Our data have shown that neutrophil recruitment was much less important in peritoneal cavity of PN-1-/- mice than in those of WT mice. These novel findings suggest that PN-1 is a serpin regulating positively PMNs functions

APA, Harvard, Vancouver, ISO, and other styles

Dissertations / Theses on the topic 'Protest song'

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles