Relevant bibliographies by topics / Protoplast fusion technology

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Academic literature on the topic 'Protoplast fusion technology'

Author: Grafiati
Published: 5 June 2025
Last updated: 25 June 2025

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Journal articles on the topic "Protoplast fusion technology"

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Gleddie, Stephen, and Wilfred A. Keller. "Protoplast fusion technology." Journal of Tissue Culture Methods 12, no. 4 (1989): 157–61. http://dx.doi.org/10.1007/bf01404443.

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S., V. S. S. S. L. N. Hima Bindu, Samatha B, and A. Singara Charya M. "A study on strain improvement of Fomitopsis feei by protoplast fusion technology." International journal of Microbiology and Mycology (IJMM) 6, no. 1 (2017): 9–15. https://doi.org/10.5281/zenodo.8394142.

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Protoplast fusion technology was used for the formation of improved strain from a brown rot fungus,&nbsp;<em>Fomitopsis feei</em>&nbsp;and a white rot fungus,&nbsp;<em>Pycnoporous</em>&nbsp;sps. for the enhanced production of exopolysaccharide. Self-fusion of&nbsp;<em>Fomitopsis feei</em>&nbsp;and intergeneric hybridization of&nbsp;<em>Fomitopsis feei</em>&nbsp;with&nbsp;<em>Pycnoporus&nbsp;</em>sp. were researched. A combination of chitinase and lysing enzymes were used for the release of protoplasts. Fusion was successful with the formation of visible stable morphological regenerated colonies after several subcultures. Mycelial growth and hyphal size of wild species and fusants were significantly different from those of the parental strains. Although, Self and intergeneric protoplast fusants were produced successfully but were not best producers of exopolysaccharide compared to wild fungi. Even though, strain improvement towards highest production of exopolysaccharide is not successful, this study shows the possibility of fusion between these two mushrooms and could be useful in other research areas. Published by the&nbsp;&nbsp;<strong>International journal of Microbiology and Mycology (IJMM)</strong>
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Li, Jintao, Linling Liu, Lin Xu, et al. "Interspecific Hybridization between Ganoderma lingzhi and G. resinaceum by PEG-Induced Double-Inactivated Protoplast Fusion." Horticulturae 9, no. 10 (2023): 1129. http://dx.doi.org/10.3390/horticulturae9101129.

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Ganoderma lingzhi is an important medicinal fungus, and it is particularly important to select strains with high yields and active substance contents. In this study, protoplasts of G. lingzhi were thermally inactivated to destroy intracellular enzyme proteins and preserve DNA. The DNA of G. resinaceum was damaged by ultraviolet (UV) radiation, and other components of the protoplasm except DNA were preserved. Then, the protoplast was induced using polyethylene glycol (PEG) for fusion. The results showed that the optimal thermal inactivation conditions for G. lingzhi were 30 min in a 45 °C water bath, and the optimal UV inactivation conditions for G. resinaceum were 70 s of irradiation using a 20 W UV lamp at a vertical distance of 15 cm. Antagonistic tests, internal transcribed space (ITS) and mitochondrial DNA identification, intersimple sequence repeat (ISSR) molecular markers and morphology were used to distinguish the parents from the fusants. Four true fusants were obtained, and the yield was 2.5%. The fruiting body yield of the fusants was significantly higher than that of G. lingzhi, and the polysaccharide and triterpene contents of the RAD-64 fusant were significantly higher than those of G. lingzhi. The results presented in this paper show that protoplast fusion technology can effectively improve G. lingzhi varieties and support the breeding of new varieties.
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Sukmadjaja, Deden, Novianti Sunarlim, Endang G. Lestari, Ika Roostika, and Tintin Suharlini. "Teknik Isolasi dan Kultur Protoplas Tanaman Padi." Jurnal AgroBiogen 3, no. 2 (2016): 60. http://dx.doi.org/10.21082/jbio.v3n2.2007.p60-65.

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&lt;p&gt;Protoplast&lt;br /&gt;fusion or somatic hybridization technology is an alternative&lt;br /&gt;technology for production hybrids of plants that are difficult&lt;br /&gt;to be produced by conventional methods due to their sexual&lt;br /&gt;incompatibility. An experiment was conducted to develop&lt;br /&gt;techniques for isolation, purification, and culture of rice&lt;br /&gt;protoplasts of cultivar IR64 and a wild rice species (Oryza&lt;br /&gt;officinalis). Optimization of protoplast isolation and purification&lt;br /&gt;methods from both rice genotypes were successfully&lt;br /&gt;done. The highest protoplast density was obtained by&lt;br /&gt;digesting embryonic callus or stems of young seedling in an&lt;br /&gt;enzyme solution containing of 2% cellulose, 0.1% pectolyase,&lt;br /&gt;0.5% macerozyme, 0.5% driselase, 5 mM ES, and 13% mannitol&lt;br /&gt;in CPW solution. The protoplast digestion was done for&lt;br /&gt;three hours by soaking in the enzyme solution followed by&lt;br /&gt;shaking at 50 rpm under a room temperature. Purification of&lt;br /&gt;the protoplasts were done by separating them from plant&lt;br /&gt;debris using a 25% sucrose solution. Protoplast regeneration&lt;br /&gt;was not successful using although different media compositions&lt;br /&gt;and conditions. Growth process from cell division to&lt;br /&gt;cell aggregate was only successful on IR64 protoplast culture&lt;br /&gt;on a medium that contained AgNO3.&lt;/p&gt;
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Polivanova, O. B., A. S. Egorova, A. B. Sivolapova, and S. V. Goryunova. "Current state and prospects of protoplast technology and potato somatic hybridization (review)." Agricultural Science Euro-North-East 24, no. 1 (2023): 7–19. http://dx.doi.org/10.30766/2072-9081.2023.24.1.7-19.

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Wild Solanum species have often been used as sources of important agricultural traits, including resistance to various diseases, pests, and abiotic factors. However, their large-scale use in potato breeding is limited by complex barriers of sexual incompatibility with Solanum tuberosum. Fusion of protoplasts enzymatically isolated from somatic cells is one of the approaches to overcoming sexual incompatibility. The diverse nuclear and cytoplasmic traits exhibited by potato somatic hybrids provide new genetic material for breeding programs, which is confirmed by the creation of a large number of somatic hybrids of cultivated potatoes with wild Solanum species. The research in development of somatic potato hybrids by means of protoplast fusion has been carried out for more than 40 years already. In this review, the prospects for the use of this technology in modern potato breeding are considered. Genomic, transcriptomic, and proteomic studies provide further insight into the fundamental processes underlying the somatic hybrids formation, such as cell wall formation, chromosomal rearrangements in fusion products, regeneration, and also make a significant contribution to understanding the processes of genome stabilization. Improvement in the methods of molecular screening of both genome and cytoplasm also contributes to the expansion of the field of application of somatic hybrids in breeding. Finally, it has been shown that somatic hybridization promotes the introgression of important agricultural traits, primarily resistance to pathogens.
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Raikar, S. V., M. C. Christey, A. J. Conner Braun, and C. Bryant. "Protoplast isolation, colony formation and shoot regeneration from Lolium perenne." NZGA: Research and Practice Series 12 (January 1, 2006): 41–44. http://dx.doi.org/10.33584/rps.12.2006.3045.

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Lolium perenne (ryegrass) is the most widely sown pasture grass in New Zealand providing forage for millions of sheep and cattle. Genetic improvement of Lolium species through conventional breeding has been particularly difficult because of its outbreeding and heterozygous nature. Biotechnology techniques that are not based on genetic modification technology, such as protoplast fusion, could help in the breeding of L. perenne as it enables the introgression of DNA (and associated desirable traits) from other species of plants that are otherwise unobtainable by conventional means. We intend to adapt this technology for L. perenne and report on our progress with the isolation of protoplasts, micro colony and callus formation, and shoot regeneration. Friable callus was induced from L. perenne seeds and used for isolation of protoplasts. After testing several combinations of enzyme mixtures at different molarities, a high yield of protoplasts (1.1 × 107 g-1 FW) with a viability of 82% has been obtained. Callus colonies were formed from protoplasts cultured on a nitrocellulose membrane over a feeder layer. Plating efficiencies of 0.1-0.2% were obtained. Regeneration of plantlets from these colonies were obtained on Linsmaier and Skoog medium supplemented with growth hormones. All the plants regenerated to date have an albino appearance.
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Chen, Sheng, Yu Li, Wen Bin Jin, Yan Chen, Xiao Guang Liu, and Fu Ping Lu. "Selective Breeding of Oxygen-Tolerant and Oxalate-Degrading Lactic Acid Bacteria by Protoplast Fusion." Advanced Materials Research 750-752 (August 2013): 1489–94. http://dx.doi.org/10.4028/www.scientific.net/amr.750-752.1489.

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Bifidobacterium lactiswith oxalate-degrading capacity can efficiently reduce the oxalate in vivo, and it can be used to prevent and treat kidney stone diseases. WhileBifidobacterium lactisis poorly oxygen-tolerant, which hinders it from being as microbial ecological agents. To obtain oxygen-tolerant and oxalate-degrading lactic acid bacteria, protoplast fusion technology was used betweenB. lactisandL. acidophilus.Under the optimum conditions of protoplast fusion with PEG 6000 concentration 50%, the fusion time 7 min, the fusion temperature 30°C, the concentration of CaCl20. 02mol/ L and the concentration of MgCl20.5mol/ L, the fusion rate reached 7.6%, and three oxygen-tolerant fusant showing that the level of oxalate degradation were similar withB. lactiswas obtained. The fusants of SZY1-7 and SZY2-1 could tolerance to pH 2.5 and 0.5% (w/v) bile salt.
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Liu, Shuping, Xiaojie Li, Jiani Zhu, et al. "Modern Technologies Provide New Opportunities for Somatic Hybridization in the Breeding of Woody Plants." Plants 13, no. 18 (2024): 2539. http://dx.doi.org/10.3390/plants13182539.

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Advances in cell fusion technology have propelled breeding into the realm of somatic hybridization, enabling the transfer of genetic material independent of sexual reproduction. This has facilitated genome recombination both within and between species. Despite its use in plant breeding for over fifty years, somatic hybridization has been limited by cumbersome procedures, such as protoplast isolation, hybridized-cell selection and cultivation, and regeneration, particularly in woody perennial species that are difficult to regenerate. This review summarizes the development of somatic hybridization, explores the challenges and solutions associated with cell fusion technology in woody perennials, and outlines the process of protoplast regeneration. Recent advancements in genome editing and plant cell regeneration present new opportunities for applying somatic hybridization in breeding. We offer a perspective on integrating these emerging technologies to enhance somatic hybridization in woody perennial plants.
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Christiansen, C., H. Kryvi, P. Sontum, and T. Skotland. "Isolation of intergeneric hybrids between Bacillus subtilis and Zymomonas mobilis and the production of thermostable amylase by hybrids." Biotechnology and Applied Biochemistry 20, no. 1 (1994): 109–16. http://dx.doi.org/10.1111/j.1470-8744.1994.tb00309.x.

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Stable hybrids were obtained by protoplast fusion between Bacillus subtilis and Zymomonas mobilis. All the hybrids were able to hydrolyse starch and possessed ampicillin‐ and tetracycline‐resistant phenotypes. Two of the hybrids, BZ‐1 and BZ‐2, were hyperproducers of alpha‐amylase. The enzyme produced by these hybrids exhibited increased thermostability. The results show that stable intergeneric gene transfer can be achieved through poly(ethylene glycol)‐mediated protoplast fusion between two industrially important genera of bacteria.
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Prajwalitha, Kanala, Vijay Bahadur, and Saket Mishra. "Somatic Hybrids and Cybrids: Innovations in Vegetable Improvement." International Journal of Plant & Soil Science 37, no. 1 (2025): 331–46. https://doi.org/10.9734/ijpss/2025/v37i15275.

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Somatic hybridization through protoplast fusion is indeed a promising method for producing asymmetrical and unbalanced polyploid somatic hybrids in various plant species. This technique involves merging protoplasts from different plant species to create hybrids that possess desirable traits for both scion and rootstock improvements. By circumventing the limitations of sexual hybridization, such as male/female sterility and sexual incompatibility, somatic hybridization enables the incorporation of beneficial genes from closely related or even distantly related species. The success of somatic hybridization in horticulture is evident in various crops such as citrus, potato, brinjal (eggplant), tomato, mango, avocado, banana, strawberry, pear, and cherry. It facilitates the transfer of numerous uncloned genes that confer resistance to biotic and abiotic stresses, thereby enhancing crop resilience and productivity. Unlike transgenic technology, which is often subject to regulatory constraints, somatic hybridization allows for the exchange of genetic material without the same legal formalities. Despite its potential benefits, somatic hybridization faces challenges and constraints compared to sexual hybridization. These include technical difficulties in protoplast isolation and fusion, as well as limitations in generating fertile hybrids and maintaining genome stability. However, advancements in genomic technologies provide optimism for overcoming these challenges. Improved understanding of plant genomes enables more precise manipulation and selection of desired traits through somatic hybridization. In conclusion, somatic hybridization is a crucial tool in modern plant breeding and crop improvement efforts. It expands the gene pool available for breeding programs by incorporating genetic variability from diverse sources, thus offering new opportunities to enhance agricultural productivity and sustainability. As research and technology continue to advance, somatic hybridization holds promise for addressing current and future challenges in global agriculture.
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Book chapters on the topic "Protoplast fusion technology"

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Sumeet and K. G. Mukerji. "Exploitation of Protoplast Fusion Technology in Improving Biocontrol Potential." In Biocontrol Potential and its Exploitation in Sustainable Agriculture. Springer US, 2000. http://dx.doi.org/10.1007/978-1-4615-4209-4_4.

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Rokka, Veli-Matti. "Protoplast Technology in Genome Manipulation of Potato Through Somatic Cell Fusion." In Somatic Genome Manipulation. Springer New York, 2015. http://dx.doi.org/10.1007/978-1-4939-2389-2_10.

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Keskitalo, Marjo Kristiina. "Application of Protoplast Fusion Technology to Tansy (Tanacetum vulgare L.): Biodiversity as a Source to Enhance Biological Activity of Secondary Compounds." In Breeding Research on Aromatic and Medicinal Plants. CRC Press, 2024. http://dx.doi.org/10.1201/9781003573227-29.

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"Applications of protoplast technology." In Plant Cell Culture, edited by N. W. Blackhall, M. R. Davey, and J. B. Power. Oxford University PressOxford, 1995. http://dx.doi.org/10.1093/oso/9780199634033.003.0003.

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Abstract Somatic hybridization of plants involves four discrete stages; protoplast isolation, protoplast fusion, the regeneration of plants from selected tissues, and analysis of regenerated plants. As virtually any combination of protoplasts can be induced to undergo fusion, somatic hybridization provides a means to circumvent sexual barriers to plant breeding. It not only provides a method for generating hybrids between sexually incompatible plants, but also facilitates the genetic modification of vegetatively propagated crops, sterile or subfertile species, and plants with naturally long life cycles. Fusion treatment results in the production of heterokaryons and homokaryons, while some protoplasts remain unfused. Heterokaryons are the fusion products relevant to plant genetic manipulation. They contain the nuclei of the two genera, species, or varieties, initially in a mixed cytoplasm. Heterokaryons may develop into hybrid cells. Like unfused plant cells, somatic hybrid cells are totipotent and are therefore capable of developing, via embryogenesis or organogenesis, into plants.
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Conference papers on the topic "Protoplast fusion technology"

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Shiwei Wang, Qinghui Wang, Ying Zhai, and Min Wang. "Screening of NHase high-producing Rhodococcus ruber Strain TQD-58 by both parents inactivated protoplast fusion." In 2012 International Symposium on Information Technology in Medicine and Education (ITME 2012). IEEE, 2012. http://dx.doi.org/10.1109/itime.2012.6291408.

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