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Journal articles on the topic 'Protoplast fusion'

Author: Grafiati
Published: 4 June 2021
Last updated: 25 July 2025

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1

Navrátilová, B. "Protoplast cultures and protoplast fusion focused on Brassicaceae: A review." Horticultural Science 31, No. 4 (2011): 140–57. http://dx.doi.org/10.17221/3809-hortsci.

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The subjects of this article are protoplast isolations and protoplast fusions, in particular their history, a review of factors influencing the protoplasts isolation and fusion, selection of hybrid plants and utilization of somatic hybrids in plant breed-ing. Somatic hybridization through protoplast fusion can overcome sexual incompatibility among plant species or genera; transfer genes of resistance to diseases (viral, bacterial, fungal), pests, herbicides and others stress factors; obtain cybrid plants; transfer cytoplasmic male sterility or incease content of secondary metabolites in hybrid
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2

Perera, Srini C., and Peggy Ozias-Akins. "Regeneration from Sweetpotato Protoplasts and Assessment of Growth Conditions for Flow-sorting of Fusion Mixtures." Journal of the American Society for Horticultural Science 116, no. 5 (1991): 917–22. http://dx.doi.org/10.21273/jashs.116.5.917.

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Petiole protoplasts of the sweetpotato [Ipomoea batatas (L.) Lam.] cultivars Red Jewel and Georgia Jet formed cell walls within 24 hours and divided in 2 to 3 days. Pretreating enzyme solutions with activated charcoal increased the viability and division frequency of protoplasts. Culture of protoplast-donor plants in a medium containing STS did not affect plant growth, protoplasm yield, or viability, but did increase the division frequency. Culture of protoplasts for 24 hours in a medium containing DB, a cell wall synthesis inhibitor, or staining of protoplasts with FDA did not significantly a
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3

Liu, Donglong, and Nancy A. Reichert. "PROTOPLAST ISOLATION AND CULTURE OF KENAF (HIBISCUS CANNABINUS L.)." HortScience 29, no. 7 (1994): 729e—729. http://dx.doi.org/10.21273/hortsci.29.7.729e.

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Protoplast isolation and culture protocols were developed for leaf tissue from 6 kenaf cultivars [Everglades 41 (E41), E71, Guatemala 4 (G4), G45, G51, and Tainung 1]. For protoplast isolation, the best combination of hydrolytic enzymes was cellulysin (1% w/v; Calbiochem) plus macerase (0.5% w/v; Calbiochem), with a 24 hour digestion at 30°C in the dark. Yields reached 7.2 (10)6 protoplasts/g leaf tissue. Protoplast viabilities ranged from 65% to 96%. Minor cultivar differences were observed related to protoplast yield, but all viability estimates were in an acceptable range. Greatest cell div
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4

Ahmed, Mohamed A. A., Miao Miao, Emmanouil D. Pratsinakis, et al. "Protoplast Isolation, Fusion, Culture and Transformation in the Woody Plant Jasminum spp." Agriculture 11, no. 8 (2021): 699. http://dx.doi.org/10.3390/agriculture11080699.

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Plant protoplasts are significant for plant cell culture, somatic cell fusion, genetics, and breeding studies. In addition, in vitro plant regeneration has great importance for developmental biology, manifesting potential applications in agriculture and biotechnology. In this regard, we present a well-established protocol regarding protoplast isolation, cell culture and protoplast fusion of Jasminum spp. In particular, different tissues of Jasminum samab L. and Jasminum mesnyi were employed for protoplast isolation, and stem explants provided a high callus induction rate in a short period of t
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5

Armita, Devi. "Plant Breeding Through Protoplast Fusion." Jurnal Biologi UNAND 8, no. 2 (2020): 42. http://dx.doi.org/10.25077/jbioua.8.2.42-47.2020.

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Protoplast culture (protoplast fusion) is one method of tissue culture that is widely used in plant breeding programs in a relatively short time. This method is used to overcome the problem of plants that are difficult or impossible to cross conventionally as well as used for species improvement by transferring the desired gene from the donor plant to the target plant via protoplast fusion. Protoplast fusion makes it possible to produce plants that are resistant to a disease and various abiotic stresses, rapid growth rates and have a better quantity and quality of metabolites than their parent
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6

Seidel, Thorsten, Philipp Johannes Artmann, Ioannis Gkekas, Franziska Illies, Anna-Lena Baack, and Martina Viefhues. "Microfluidic Single-Cell Study on Arabidopsis thaliana Protoplast Fusion—New Insights on Timescales and Reversibilities." Plants 13, no. 2 (2024): 295. http://dx.doi.org/10.3390/plants13020295.

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Plant cells are omnipotent and breeding of new varieties can be achieved by protoplast fusion. Such fusions can be achieved by treatment with poly(ethylene glycol) or by applying an electric field. Microfluidic devices allow for controlled conditions and targeted manipulation of small batches of cells down to single-cell analysis. To provide controlled conditions for protoplast fusions and achieve high reproducibility, we developed and characterized a microfluidic device to reliably trap some Arabidopsis thaliana protoplasts and induced cell fusion by controlled addition of poly(ethylene glyco
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7

Nolan, Richard A. "Stage-specific changes in cytoplasmic protein synthesis in Entomophaga aulicae protoplasts." Canadian Journal of Microbiology 35, no. 3 (1989): 373–78. http://dx.doi.org/10.1139/m89-057.

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The patterns of protein synthesis associated with three sequential stages in protoplast morphogenesis (spindle-shaped, early fusion sphere, and late fusion sphere protoplasts) of the fungus Entomophaga aulicae were studied using both one-dimensional gels with general protein staining and two-dimensional gels with [35S]methionine protein labelling and fluorography. A total of 332 proteins were observed with 63.5% (211) common to all three developmental stages. Of the individual totals, 3.3% (8 out of 245), 7.3% (22 out of 301), and 4.5% (13 out of 286) of the proteins were unique to the spindle
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8

Chen, Qin, H. Y. Li, Y. Z. Shi, D. Beasley, B. Bizimungu, and M. S. Goettel. "Development of an effective protoplast fusion system for production of new potatoes with disease and insect resistance using Mexican wild potato species as gene pools." Canadian Journal of Plant Science 88, no. 4 (2008): 611–19. http://dx.doi.org/10.4141/cjps07045.

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Somatic hybridization through protoplast fusion is an important alternative approach for overcoming sexual incompatibility between diploid Solanum species and cultivated potatoes. However, compared with other potato species, methods for protoplast isolation and plant generation for several Mexican wild diploid potato species are not well established. In this study, a systematic procedure was designed for the isolation of a large number of high-quality protoplasts from various Mexican wild species that carry high levels of disease (late blight) and insect [Colorado potato beetle (CPB)] resistan
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9

Djajanegara, Ira, and Agus Masduki. "PROTOPLAST FUSION BETWEEN WHITE AND BROWN OYSTER MUSHROOMS." Indonesian Journal of Agricultural Science 11, no. 1 (2013): 16. http://dx.doi.org/10.21082/ijas.v11n1.2010.p16-23.

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Genetic crossing of white oyster mushroom (Pleurotus floridae) to introduce longer storage life trait can only be done within individuals in this particular species. However, longer storage life trait is possessed by brown oyster mushroom (Pleurotus cystidiosus). Therefore, a protoplast fusion experiment between white and brown oyster mushrooms was conducted to obtain an oyster mushroom strain showing high productivity and long storage life. The experiment was done at the biology laboratory of the University of Al Azhar Indonesia from May 2008 to August 2009. Protoplast fusion was done by isol
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10

Nolan, Richard A. "Influence of a negatively charged surface (Teflon disk) on Entomophaga aulicae protoplast morphogenesis under mass fermentation conditions." Canadian Journal of Botany 69, no. 11 (1991): 2578–81. http://dx.doi.org/10.1139/b91-321.

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The effects of a negatively charged surface (Teflon disk) on protoplast morphogenesis for the fungus Entomophaga aulicae under mass fermentation conditions were determined. The control consisted of a vessel lacking such a disk. In the presence of the disk the initial three and sequentially produced protoplast stages (spindle shaped, early fusion sphere, and late fusion sphere protoplasts) recycled with the early fusion sphere predominating. The production of the subsequent and walled stage (i.e., hyphal body) was suppressed. The results are in contrast with those obtained in a previous study u
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11

Wang, Chao, XiaoLin Zhang, Zhi Chen, Ying Wen, and Yuan Song. "Strain construction for enhanced production of spinosad via intergeneric protoplast fusion." Canadian Journal of Microbiology 55, no. 9 (2009): 1070–75. http://dx.doi.org/10.1139/w09-064.

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Spinosad is a new class of insecticides produced by Saccharopolyspora spinosa . The aim of this study was to construct a starch-utilizing strain that overproduced spinosad by intergeneric fusion between S. spinosa and Streptomyces avermitilis . Protoplast fusion is an important technique for engineering microbial strains, especially for microorganisms with few available molecular genetic tools. Protoplast fusion was conducted with UV-irradiated protoplasts of S. spinosa and S. avermitilis. Among 76 recombinants screened by ESI-MS and HPLC, a starch-utilizing strain F17, identified as S. spinos
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12

Pauls, K. P., and P. V. Chuong. "Flow cytometric identification of Brassica napus protoplast fusion products." Canadian Journal of Botany 65, no. 5 (1987): 834–38. http://dx.doi.org/10.1139/b87-113.

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A flow cytometric method to identify Brassica somatic hybrids has been developed. The procedure is based on the use of fluorescein isothiocyanate stained hypocotyl protoplasts and unstained mesophyll protoplasts as partners for polyethylene glycol induced protoplast fusion. The fluorescein isothiocyanate stained hypocotyl protoplasts and unstained mesophyll protoplasts were passed through the flow cytometer – cell sorter separately to maximize the sensitivity of the red and green detectors to chlorophyll and fluorescein isothiocyanate fluorescence, respectively, and to ensure that there was no
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13

S., V. S. S. S. L. N. Hima Bindu, Samatha B, and A. Singara Charya M. "A study on strain improvement of Fomitopsis feei by protoplast fusion technology." International journal of Microbiology and Mycology (IJMM) 6, no. 1 (2017): 9–15. https://doi.org/10.5281/zenodo.8394142.

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Protoplast fusion technology was used for the formation of improved strain from a brown rot fungus,&nbsp;<em>Fomitopsis feei</em>&nbsp;and a white rot fungus,&nbsp;<em>Pycnoporous</em>&nbsp;sps. for the enhanced production of exopolysaccharide. Self-fusion of&nbsp;<em>Fomitopsis feei</em>&nbsp;and intergeneric hybridization of&nbsp;<em>Fomitopsis feei</em>&nbsp;with&nbsp;<em>Pycnoporus&nbsp;</em>sp. were researched. A combination of chitinase and lysing enzymes were used for the release of protoplasts. Fusion was successful with the formation of visible stable morphological regenerated colonie
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14

Kurita-Tashiro, Asami, Noriko Hayashi, Tomoya Oyanagi, and Hamako Sasamoto. "New Factors for Protoplast-Callose-Fiber Formation in Salt-Tolerant Mangrove Plants, Avicennia alba and Bruguiera sexangula and Analysis of Fiber Substructures." Journal of Plant Studies 9, no. 2 (2020): 1. http://dx.doi.org/10.5539/jps.v9n2p1.

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Elongated and spiral &amp;beta;-1,3-glucan (callose) fibers were obtained by new factors from protoplasts cultured in liquid medium from suspension cultured cells of two salt-tolerant mangrove species; Avicennia alba and Bruguiera sexangula. Differences in salt factor for protoplast-fiber formation were compared with those of the callose fibers developed from protoplasts of non-mangrove tree plants, Larix leptolepis and Betula platyphylla, which high concentrations of divalent cations, Mg2+ (50 mM) or Ca2+ (100 mM), were stimulatory. In the halophilic A. alba protoplasts, whose cell division w
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15

Djajanegara, Ira, and Agus Masduki. "PROTOPLAST FUSION BETWEEN WHITE AND BROWN OYSTER MUSHROOMS." Indonesian Journal of Agricultural Science 11, no. 1 (2013): 16. http://dx.doi.org/10.21082/ijas.v11n1.2010.16-23.

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Genetic crossing of white oyster mushroom (Pleurotus floridae)&lt;br /&gt;to introduce longer storage life trait can only be done within&lt;br /&gt;individuals in this particular species. However, longer storage&lt;br /&gt;life trait is possessed by brown oyster mushroom (Pleurotus&lt;br /&gt;cystidiosus). Therefore, a protoplast fusion experiment between&lt;br /&gt;white and brown oyster mushrooms was conducted to obtain an&lt;br /&gt;oyster mushroom strain showing high productivity and long&lt;br /&gt;storage life. The experiment was done at the biology laboratory&lt;br /&gt;of the Universit
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16

Zhang, Shiying, Sujuan Guo, and Ruijie Zheng. "Optimization of Establishment, Protoplast Separation, and Fusion via Embryonic Suspension System in Chestnut (Castanea mollissima Bl.)." Agronomy 15, no. 7 (2025): 1595. https://doi.org/10.3390/agronomy15071595.

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Castanea mollissima Bl. is rich in nutrition and strong in stress resistance, and has nutritional, economic, and ecological values. A protoplast is impactful in somatic fusion and germplasm creation. Here, we propose an effective scheme for the construction of an embryonic suspension cell, protoplast isolation, and fusion. Studies have shown that when 1.0 g yellow loose embryonic callus was inoculated into MS + 1.5 mg∙L−1 6-BA + 0.2 mg∙L−1 NAA + 0.5 mg∙L−1 2, 4-D liquid medium, a stable suspension cell line can be obtained. After further culturing for 2–4 days, protoplast isolation was perform
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17

Tusa, N., J. W. Grosser, and F. G. Gmitter. "Plant Regeneration of `Valencia' Sweet Orange, `Femminello' Lemon, and the Interspecific Somatic Hybrid following Protoplasm Fusion." Journal of the American Society for Horticultural Science 115, no. 6 (1990): 1043–46. http://dx.doi.org/10.21273/jashs.115.6.1043.

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Protoplasm culture following the chemical fusion of `Valencia' sweet orange [Citrus sinensis (L.) Osb.] protoplasts, isolated from an embryogenic suspension culture, with `Femminello' lemon [Citrus limon (L.) Burro. f.] leaf protoplasts resulted in the regeneration of an interspecific allotetraploid somatic hybrid plant, two autotetraploid lemon plants, and diploid plants from both parents. The regeneration of plants from lemon leaf protoplasts is an example of protoplast-to-plant regeneration from non-nucellus-derived tissue for Citrus. Regenerated plants were classified according to leaf mor
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18

Kucsera, Judit, Ilona Pfeiffer, and Lajos Ferenczy. "A novel method for hybridization of Saccharomyces species without genetic markers." Canadian Journal of Microbiology 44, no. 10 (1998): 959–64. http://dx.doi.org/10.1139/w98-093.

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Protoplasts of Saccharomyces cerevisiae were inactivated by treatment with different concentrations of antifungal compounds for various periods. Of the 14 compounds tested, N-ethylmaleimide proved to be the most efficient. The inactivation effect was fully reproducible. The inactivated protoplasts could be reactivated and still function as fusion partners. They were fused with untreated protoplasts by polyethylene glycol treatment and produced viable hybrid cells. Nuclear and extrachromosomal genetic analysis and chromosome separation of the fusion products from fusion experiments involving in
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19

Li, Jintao, Linling Liu, Lin Xu, et al. "Interspecific Hybridization between Ganoderma lingzhi and G. resinaceum by PEG-Induced Double-Inactivated Protoplast Fusion." Horticulturae 9, no. 10 (2023): 1129. http://dx.doi.org/10.3390/horticulturae9101129.

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Ganoderma lingzhi is an important medicinal fungus, and it is particularly important to select strains with high yields and active substance contents. In this study, protoplasts of G. lingzhi were thermally inactivated to destroy intracellular enzyme proteins and preserve DNA. The DNA of G. resinaceum was damaged by ultraviolet (UV) radiation, and other components of the protoplasm except DNA were preserved. Then, the protoplast was induced using polyethylene glycol (PEG) for fusion. The results showed that the optimal thermal inactivation conditions for G. lingzhi were 30 min in a 45 °C water
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20

Al-Nema, Qutaiba, and Mozahim AL-Mallah. "Electrofusion of mesophyll protoplasts from two varieties of sugar beet, (Beta vulgaris L.)." Journal of Life and Bio Sciences Research 1, no. 1 (2020): 22–25. http://dx.doi.org/10.38094/jlbsr117.

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Somatic hybridization between different plants through protoplast fusion represent an efficient experimental approach to produce genetically transformed plant species. Electrofution of mesophyll protoplasts in sugar beet was occurred to overcome the barriers faced breeding program of this economically industrial crop Protoplasts were successfully isolated from leave's mesophyll of two varieties of sugar beet (Beta vulgaris L.). Various enzyme solutions were assessed for the cell wall degrading ability. They express different efficiency in isolation of mesophyll protoplasts of var. Baraka. The
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21

Hassan, Mohamed M., Ismail A. Ismail, and Abd El-Latif A. Sorour. "Phylogeny and antagonistic activity of some protoplast fusants in Trichoderma and Hypocrea." International Journal of Applied Sciences and Biotechnology 2, no. 2 (2014): 146–51. http://dx.doi.org/10.3126/ijasbt.v2i2.10113.

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The present work aimed to apply mutagenesis and inter-specific protoplast fusion techniques of two locally isolated Hypocrea and Trichoderma to enhancement their biocontrol abilities against some important plant fungal pathogens which cause damping-off diseases that attacking different crops. The mutants were selected after EMS/UV treatment of Trichoderma isolates. The obtained protoplasts were fused by polyethylene glycol, and six fusants were selected for further studies. The phylogeny of the parental strains was carried out using sequence of ITS region. The BLAST of the obtained sequence wa
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22

Al-Ne'ma, Q. Sh, and M. K. Al-Mallah. "Protoplast isolation from leaf mesophyll of sugarbeet Beta vulgaris L. axenic seedlings." Journal of Biotechnology Research Center 7, no. 3 (2013): 36–42. http://dx.doi.org/10.24126/jobrc.2013.7.3.280.

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Protoplasts were isolated from leaf mesophyll of sugarbeet (Beta vulgaris L.) axenic seedlings. Eight enzyme mixtures were tested for cell wall degrading ability. The efficient enzyme solution was mixture "II" that consist of 1.5% Cellulase RS, 2% Cellulase R10, 1% Macerozym R10 and 0.1% Pectolyase Y23. This mixture was efficient in releasing protoplasts and gave high yield of a density 7.3 × 104 protoplast / ml. These isolated protoplasts were viable 93%, their sizes ranged from 13 up to 52 µm, vacuolated and unvacuolated. This finding enable workers to focus on somatic hybridization through
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23

Zhao, Lihong, Wenli Yin, and Lele Wang. "High-Yield Laccase-Producing Strains Constructed by Protoplast Fusion Between Bacterium and Fungus." Open Biotechnology Journal 9, no. 1 (2015): 221–24. http://dx.doi.org/10.2174/1874070701509010221.

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This study is based on the construction of high-yield laccase-producing fusant achieved by inter-kingdom protoplast fusion between Pleurotus ostreatus and Escherichia coli. The optimized protoplasts formation and regeneration conditions were demonstrated with the presence of 1.5% cellulase +1.0% snailase and 0.6M mannitol at 30°C for 3h. The fusants were screened for different characteristics between two parental strains and further identified by laccase activity, offering one of the genetically stable fusants, Strain F. The fusant F produced the highest yield of laccase, being about 22% highe
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24

Suryati, Emma, Andi Tenriulo, and Sri Rejeki Hesti Mulyaningrum. "ISOLASI DAN KULTUR PROTOPLAS RUMPUT LAUT Kappaphycus alvarezii DI LABORATORIUM." Jurnal Riset Akuakultur 2, no. 3 (2007): 399. http://dx.doi.org/10.15578/jra.2.3.2007.399-405.

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Isolasi protoplas rumput laut K. alvarezii, telah dilakukan dalam rangka penyiapan protoplas untuk penyilangan melalui fusi protoplas. Metode yang digunakan antara lain melalui cara kimia dengan melisis tallus rumput laut dengan campuran enzim komersial, kemudian enzim yang berasal dari viscera keong mas baik yang segar maupun yang beku, dengan media kultur yang digunakan pada pemeliharaan makro algae antara lain Conwy, PES, dan air laut steril. Tallus rumput laut yang digunakan berasal dari bagian pangkal, tengah dan ujung. Protoplas yang hidup diuji menggunakan evans blue 0,1%, hormon perang
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25

Wang, Peilin, Yuanchun Pu, Muhammad Ali Abid, et al. "A Rapid and Efficient Method for Isolation and Transformation of Cotton Callus Protoplast." International Journal of Molecular Sciences 23, no. 15 (2022): 8368. http://dx.doi.org/10.3390/ijms23158368.

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Protoplasts, which lack cell walls, are ideal research materials for genetic engineering. They are commonly employed in fusion (they can be used for more distant somatic cell fusion to obtain somatic hybrids), genetic transformation, plant regeneration, and other applications. Cotton is grown throughout the world and is the most economically important crop globally. It is therefore critical to study successful extraction and transformation efficiency of cotton protoplasts. In the present study, a cotton callus protoplast extraction method was tested to optimize the ratio of enzymes (cellulase,
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26

Gleddie, Stephen, and Wilfred A. Keller. "Protoplast fusion technology." Journal of Tissue Culture Methods 12, no. 4 (1989): 157–61. http://dx.doi.org/10.1007/bf01404443.

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27

Binding, Horst, Monika Zuba, Joachim Rudnick, and Gudrun Mordhorst. "Protoplast Gel Fusion." Journal of Plant Physiology 133, no. 4 (1988): 409–13. http://dx.doi.org/10.1016/s0176-1617(88)80027-0.

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28

Aljaramany, Naseem, and Sokrat G. Monakhos. "Isolation and regeneration of cell suspension-derived Foeniculum vulgare protoplasts." BIO Web of Conferences 139 (2024): 05008. http://dx.doi.org/10.1051/bioconf/202413905008.

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Hybrid development incorporating lines with cytoplasmic male sterility (CMS) has become an increasingly effecting technique for utilizing heterosis in vegetable crops. Currently, somatic hybridization has become prevalent in the Apiaceae family, which includes carrots, celery, and leeks. Therefore, as a first step, a simple and efficient protocol for isolating and regenerating protoplasts is established for the commercial fennel hybrid “Dragon” as a source of CMS that will be transferred later into carrot via protoplast fusion. To this end, cell suspensions from Dragon were initiated as a sour
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Gu, Yu-Huan, and Wen-Hsiung Ko. "Creation of hybrid vigor through nuclear transplantation in Phytophthora." Canadian Journal of Microbiology 47, no. 7 (2001): 662–66. http://dx.doi.org/10.1139/w01-074.

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When isolated nuclei of a diploid oomycete, Phytophthora parasitica, were fused with protoplasts of another strain of the same species, the regenerated nuclear hybrids grew faster than the parental isolates. Such a phenomenon did not occur in hybrids regenerated from mitochondrion–protoplast or protoplast–protoplast fusion products between these two strains. These results indicate that hybrid vigor is the result of the interaction between two different kinds of nuclei, but not between mitochondria, and they suggest that the presence of mitochondria from nuclear donor cells represses the expres
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Burza, W., B. Woźniak, J. A. Tarkowska, and S. Malepszy. "Cytohistological analysis of somatic embryogenesis in cucumber (Cucumis sativus L). II. Natural fluorescence and direct somatic embryogenesis from protoplasts." Acta Societatis Botanicorum Poloniae 63, no. 3-4 (2014): 265–68. http://dx.doi.org/10.5586/asbp.1994.035.

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The development of protoplast derived from somatic embryos and some of their characteristics were compared with embryos from suspension and in vivo in the same B line. Embryos formed in a protoplast culture differed from others that their younger stages contained vacuolated cells, and older ones had altered morphological and histological structure. Somatic embryogenesis is more regular from suspension then from protoplasts. No distinct differences were observed in the rate of embryo development in vivo and in vitro, and in vitro embryos show a larger variation in size at the same stage. Embryo
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Ftouhi, N., and N. Guillén. "Genetic analysis of fusion recombinants in Bacillus subtilis: function of the recE gene." Genetics 126, no. 3 (1990): 487–96. http://dx.doi.org/10.1093/genetics/126.3.487.

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Abstract Bacillus subtilis protoplast fusion allows the study of the genetic recombination of an entire procaryotic genome. Protoplasts from bacterial strains marked genetically by chromosomal mutations were fused using polyethylene glycol and the regenerated cells analyzed. Recombinants represent 19.3% of heterozygotic cells; they are haploids. Individual characterization of clones show a unique particular phenotype in each colony suggesting that recombination takes place immediately after fusion, probably before the first cellular division. Recombination occurs in the whole chromosome; in on
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32

GLORIA, FERNANDA JANUZZI MENDES DA, FRANCISCO DE ASSIS ALVES MOURÃO FILHO, and BEATRIZ MADALENA JANUZZI MENDES. "Plant regeneration from protoplast of Brazilian citrus cultivars." Pesquisa Agropecuária Brasileira 35, no. 4 (2000): 727–32. http://dx.doi.org/10.1590/s0100-204x2000000400008.

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A procedure is described to regenerate plants from protoplasts of Brazilian citrus cultivars, after isolation, fusion and culture. Protoplasts were isolated from embryogenic cell suspension cultures and from leaf mesophyll of seedlings germinated in vitro. The enzyme solution for protoplast isolation was composed of mannitol (0.7 M), CaCl2 (24.5 mM), NaH2PO4 (0.92 mM), MES (6.15 mM), cellulase (Onozuka RS - Yakult, 1%), macerase (Onozuka R10 - Yakult, 1%) and pectolyase Y-23 (Seishin, 0.2%). Protoplast culture in liquid medium after chemical fusion lead to the formation of callus colonies furt
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33

Gyuris, J., and E. G. Duda. "High-efficiency transformation of Saccharomyces cerevisiae cells by bacterial minicell protoplast fusion." Molecular and Cellular Biology 6, no. 9 (1986): 3295–97. http://dx.doi.org/10.1128/mcb.6.9.3295-3297.1986.

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After a new transformation procedure, 10% of Saccharomyces cerevisiae cells were found to contain transforming DNA sequences. We used direct transfer of plasmid molecules by fusing bacterial minicell protoplasts to yeast protoplasts. Since the procedure significantly reduces the toxic effect of procaryotic protoplasm on the eucaryotic organism, it might be generally applicable in other systems in which transformation is inefficient or impossible.
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Gyuris, J., and E. G. Duda. "High-efficiency transformation of Saccharomyces cerevisiae cells by bacterial minicell protoplast fusion." Molecular and Cellular Biology 6, no. 9 (1986): 3295–97. http://dx.doi.org/10.1128/mcb.6.9.3295.

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After a new transformation procedure, 10% of Saccharomyces cerevisiae cells were found to contain transforming DNA sequences. We used direct transfer of plasmid molecules by fusing bacterial minicell protoplasts to yeast protoplasts. Since the procedure significantly reduces the toxic effect of procaryotic protoplasm on the eucaryotic organism, it might be generally applicable in other systems in which transformation is inefficient or impossible.
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Sukmadjaja, Deden, Novianti Sunarlim, Endang G. Lestari, Ika Roostika, and Tintin Suharlini. "Teknik Isolasi dan Kultur Protoplas Tanaman Padi." Jurnal AgroBiogen 3, no. 2 (2016): 60. http://dx.doi.org/10.21082/jbio.v3n2.2007.p60-65.

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&lt;p&gt;Protoplast&lt;br /&gt;fusion or somatic hybridization technology is an alternative&lt;br /&gt;technology for production hybrids of plants that are difficult&lt;br /&gt;to be produced by conventional methods due to their sexual&lt;br /&gt;incompatibility. An experiment was conducted to develop&lt;br /&gt;techniques for isolation, purification, and culture of rice&lt;br /&gt;protoplasts of cultivar IR64 and a wild rice species (Oryza&lt;br /&gt;officinalis). Optimization of protoplast isolation and purification&lt;br /&gt;methods from both rice genotypes were successfully&lt;br /&gt;don
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Satpute, Aditi D., Chunxian Chen, Fredrick G. Gmitter, et al. "Cybridization of Grapefruit with ‘Dancy’ Mandarin Leads to Improved Fruit Characteristics." Journal of the American Society for Horticultural Science 140, no. 5 (2015): 427–35. http://dx.doi.org/10.21273/jashs.140.5.427.

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In cybridization, new combinations of nuclear and cytoplasmic genes result in a unique genotype that may bring cellular, physical, physiological, and biochemical changes to the plant. This has been demonstrated in the unexpected cybrids generated from the fusion of citrus (Citrus sp.) protoplasts in two independent experiments. The first experiment was conducted to generate potentially seedless triploids by fusing diploid protoplasts of embryogenic ‘Dancy’ mandarin (Citrus reticulata) suspension culture cells with haploid ‘Ruby Red’ grapefruit (C. paradisi) protoplasts derived from tetrad-stag
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37

Rahmawati, Novia, Muhammad Zainuri, and Hermin Pancasakti Kusumaningrum. "Aplikasi Pakan Kaya Karotenoid Hasil Fusi ProtoplasmIntergenera Dunaliella salina dan Chlorella vulgaris pada Udang Windu (Penaeus monodon F.) Stadia PL-20 Di Desa Asempapan, Pati, Jawa Tengah." Bioma : Berkala Ilmiah Biologi 15, no. 2 (2013): 46. http://dx.doi.org/10.14710/bioma.15.2.46-52.

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Dunaliella salina and Chlorella vulgaris is a natural feed microalgae with high carotenoid content that can be increased using protoplast fusion technique. Protoplast fusion as one of the application fields of genetic engineering is a method for obtaining recombinant with the desired properties and profitable in a short time. This study aimed to see the effect of the addition of carotenoid-rich feed results from protoplast fusion recombinant D. salina and C. vulgaris on the survival rate and weight of shrimp post larvae. Mixed fusion results feed and artificial feed needed for the growth of po
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38

Fo, Francisco A. A. Mourão, Jude W. Grosser, and Frederick G. Gmitter. "362 PRODUCTION OF SEVEN NEW INTERGENERIC SOMATIC HYBRIDS FOR CITRUS ROOTSTOCK IMPROVEMENT." HortScience 29, no. 5 (1994): 482g—483. http://dx.doi.org/10.21273/hortsci.29.5.482g.

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Protoplast culture following polyethylene glycol (PEG)-induced fusion resulted in the regeneration of somatic hybrid plants from the following combinations: `Succari' sweet orange (C. sinensis L. Osbeck) + Severinia disticha; `Hamlin' sweet orange (C. sinensisj + S. disticha: `Valencia' sweet orange (C. sinesis) + S. disticha; `Nova' tangelo (C. reticulata hybrid) + S. disticha; `Succari' sweet orange + S. buxifolia; `Nova' tangelo + Citropsis gilletiana; and `Succari' sweet orange + Atlantia ceylanica. `Succari', `Hamlin', `Valencia', and `Nova' protoplasts were Isolated from ovule-derived em
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39

Kang, Yup, Jung H. Kim, and Dewey D. Y. Ryu. "Protoplast Fusion ofLactobacillus casei." Agricultural and Biological Chemistry 51, no. 8 (1987): 2221–27. http://dx.doi.org/10.1080/00021369.1987.10868376.

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40

Kohler, Joyce, and Gary Darland. "Protoplast fusion inStreptomyces avermitilis." Journal of Industrial Microbiology 3, no. 5 (1988): 311–20. http://dx.doi.org/10.1007/bf01569532.

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41

Pe'er, S., and I. Chet. "Trichoderma protoplast fusion: a tool for improving biocontrol agents." Canadian Journal of Microbiology 36, no. 1 (1990): 6–9. http://dx.doi.org/10.1139/m90-002.

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Protoplasts from two auxotrophic mutants of Trichoderma harzianum Rifai (ATCC 32173), obtained from young thalli following cell wall digestion by NovoZym 234, were fused in 33% PEG suspended in 10 mM Tris-HCl and 10 mM CaCl2, pH 7.5. The frequency of fusion between lysine- and arginine-requiring auxotrophs resulting in prototrophic strains was about 5%. These prototrophic strains were classified into parental and nonparental types. Colonies developed from single conidia of the nonparental phenotype exhibited prototrophic parental or recombinant phenotypes. The ability of both prototrophic and
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42

Cho, Kwang-Soo, and Tae-Ho Park. "Potato breeding via protoplast fusion." Journal of Plant Biotechnology 41, no. 2 (2014): 65–72. http://dx.doi.org/10.5010/jpb.2014.41.2.65.

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Ohgawara, T., and S. Kobayashi. "Application of protoplast fusion tocitrusbreeding." Food Biotechnology 5, no. 2 (1991): 169–84. http://dx.doi.org/10.1080/08905439109549800.

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KANG, Yup, Jung H. KIM, and Dewey D. Y. RYU. "Protoplast fusion of Lactobacillus casei." Agricultural and Biological Chemistry 51, no. 8 (1987): 2221–27. http://dx.doi.org/10.1271/bbb1961.51.2221.

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Kanatani, Kazuo, Kazushi Yoshida, Takatsugu Tahara, Masaru Sakamoto, and Masao Oshimura. "Intraspecific Protoplast Fusion ofLactobacillus plantarum." Agricultural and Biological Chemistry 54, no. 1 (1990): 225–27. http://dx.doi.org/10.1080/00021369.1990.10869927.

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Arnau, José, Antonio Ortiz, Juan C. Gomez-Fernández, Francisco J. Murillo, and Santiago Torres-Martínez. "Liposome-protoplast fusion inPhycomyces blakesleeanus." FEMS Microbiology Letters 51, no. 1 (1988): 37–40. http://dx.doi.org/10.1111/j.1574-6968.1988.tb02964.x.

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Iwata, M., M. Mada, and H. Ishiwa. "Protoplast fusion of Lactobacillus fermentum." Applied and Environmental Microbiology 52, no. 2 (1986): 392–93. http://dx.doi.org/10.1128/aem.52.2.392-393.1986.

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Li, Yinmei, Lijie Guan, Liren Lou, et al. "Laser-induced tobacco protoplast fusion." Science in China Series C: Life Sciences 42, no. 2 (1999): 122–27. http://dx.doi.org/10.1007/bf02880046.

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Kanchanapoom, Kamnoon, and Wendy F. Boss. "Osmoregulation of fusogenic protoplast fusion." Biochimica et Biophysica Acta (BBA) - Biomembranes 861 (1986): 429–39. http://dx.doi.org/10.1016/0005-2736(86)90451-7.

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Muralidhar, R. V., and T. Panda. "Fungal protoplast fusion – a revisit." Bioprocess Engineering 22, no. 5 (2000): 429–31. http://dx.doi.org/10.1007/s004490050755.

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