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Dissertations / Theses on the topic 'Protoplast'

Author: Grafiati

Published: 4 June 2021

Last updated: 25 July 2025

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1

Cheng, Jianping. "Inheritance of protoplast culturability and improvement in pollen development by protoplast manipulation in solanum." Diss., This resource online, 1990. http://scholar.lib.vt.edu/theses/available/etd-09162005-115010/.

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2

Brites, Anny Stella Monteiro. "Seleção de linhagens de Saccharomyces cerevisiae potencializadas pelo fator Killer, H2S- e o carater floculante." Universidade de São Paulo, 2003. http://www.teses.usp.br/teses/disponiveis/11/11141/tde-19052003-144728/.

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Dentre as características desejáveis em leveduras fermentadoras alcóolicas estão a capacidade de floculação, a não produção de H2S e o caráter "killer". Neste trabalho foram selecionadas sete linhagens de Saccharomyces cerevisiae com algumas destas características, que passaram por testes confirmativos e pela cariotipagem eletroforética resultando na escolha de duas linhagens: ATCC 26602 (altamente floculante) e K1 (H2S - e possuidoras do caráter "killer"). Estas linhagens foram utilizadas em um cruzamento via fusão de protoplasto para se obter um produto de fusão estável com as característica

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3

Wilson, J. M. "Protoplast-mediated genetic manipulation of brewing yeasts." Thesis, University of Nottingham, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.355426.

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4

Illing, G. T. "Protoplast fusion and regeneration in Streptomyces clavuligerus." Thesis, University of Nottingham, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.378980.

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5

Dunn, R. M. "The protoplast mediated genetics of thermophilic bacilli." Thesis, Cardiff University, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.380254.

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6

Mandegaran, Zohreh. "Genetic improvement of roses by protoplast fusion." Thesis, University of East London, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.339583.

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7

Seear, Paul James. "Protoplast production and interspecific hybridisation in Lupinus albus." Thesis, University of Reading, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.326751.

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8

Maren, Nathan Allen. "Symmetric Protoplast Fusion in Interserial Syringa (Oleaceae) Hybridization." Thesis, North Dakota State University, 2016. https://hdl.handle.net/10365/28026.

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Few other woody plants embody the preeminence of temperate woody plants in garden cultivation like the lilacs. In spite of their relationship, the trees lack the diversity of cultivated floral forms observed within the shrub lineages. Typical selection and cross-pollination schemes within the tree lilacs or between trees and shrubs have failed to yield the diversity of colors and fragrances on a tree form. With somatic fusion in Citrus spp. as a guideline for Syringa spp. protoplast isolation and culture, experiments were designed to optimize the conditions through somatic fusion. Protoplast i

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9

Attree, S. M. "Properties of Pteridium protoplasts." Thesis, University of Manchester, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.378009.

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10

Thompson, J. A. "Plant regeneration from cell and protoplast cultures of rice." Thesis, University of Nottingham, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.378368.

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11

Tamura, Mihoko. "Ploidy Manipulation through Protoplast Culture Techniques for Persimmon Breeding." Kyoto University, 1997. http://hdl.handle.net/2433/202415.

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12

Khehra, Gurpreet Singh. "Genetic manipulation in rice using rice tungro spherical virus coat protein genes." Thesis, University of Nottingham, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.261168.

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13

Hansen, Christine S. "Construction of galactose assimilating, carotenoid producing yeasts by protoplast fusion." Thesis, University of British Columbia, 1988. http://hdl.handle.net/2429/27935.

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Protoplasts were prepared from two yeast strains P. rhodozyma (ATCC 24202) and K. fragilis (ATCC 8455). Protoplasts prepared from P. rhodozyma were facilitated by prior growth of the cells in a media containing S-(2-aminoethyl)-L-cysteine. Protoplasts from these two yeast genera were fused either by the use of electrofusion or polyethylene glycol treatment. Stable carotenoid producing cell lines were selected by growth at 30°C on yeast nitrogen base plus galactose. Selected single fusants display taxonomic characteristics common to both genera with a cellular morphology and a carotenoid compo

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14

Finch, Robert P. "Development of protoplast systems for the genetic manipulation of rice." Thesis, University of Nottingham, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.280099.

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15

Baset, Abdul. "Protoplast to plant regeneration systems for cultivated and wild rices." Thesis, University of Nottingham, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.315069.

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16

Ghadimzadeh, Mortaza. "Studies of protoplast and liposome techniques in plant tissue culture." Thesis, University of Salford, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.255239.

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17

Foulger, David. "Protoplast regeneration and somatic hybridisation of potato (Solanum tuberosum L.)." Thesis, Rothamsted Research, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.374242.

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18

Butt, Adrian David. "Physiological and biochemical studies with rubber (Hevea brasiliensis) protoplasts." Thesis, University of Nottingham, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.257197.

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19

Nobre, Jose Manso Preto. "Studies on methods for the genetic manipulation of barley (Hordeum vulgare L.)." Thesis, University of Nottingham, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.336932.

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20

Wardrop, Julie. "Biotechnological applications of perfluorochemical liquids in plant tissue culture." Thesis, University of Nottingham, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.389475.

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21

Chen, W. H. "Genetic manipulation of sugarcane." Thesis, University of Nottingham, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.376164.

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22

Cui, Xiucheng. "Targeted Gene Editing Using CRISPR/Cas9 in a Wheat Protoplast System." Thesis, Université d'Ottawa / University of Ottawa, 2017. http://hdl.handle.net/10393/36543.

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The clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system has become a promising tool for targeted gene editing in a variety of organisms including plants. In this system, a 20 nt sequence on a single guide RNA (sgRNA) is the only gene-specific information required to modify a target gene. Fusarium head blight (FHB) is a devastating disease in wheat caused by the fungus Fusarium graminearum. The trichothecene it produces, deoxynivalenol (DON), is a major mycotoxin contaminant causing food production loss both in quality and yield. In this project, we used the CRISPR/Ca

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23

Raikar, Sanjeev Vencu. "Protoplast fusion of Lolium perenne and Lotus corniculatus for gene introgression." Phd thesis, Lincoln University. Bio-Protection and Ecology Division, 2007. http://theses.lincoln.ac.nz/public/adt-NZLIU20080214.105406/.

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Protoplast fusion of Lolium perenne and Lotus corniculatus for gene introgression by Sanjeev V. Raikar Lolium perenne is one of the most important forage crops globally and in New Zealand. Lotus corniculatus is a dicotyledonous forage that contains valuable traits such as high levels of condensed tannins, increased digestibility, and high nitrogen fixing abilities. However, conventional breeding between these two forage crops is impossible due to their markedly different taxonomic origin. Protoplast fusion (somatic hybridisation) provides an opportunity for gene introgression between these two

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24

Raikar, S. V. "Protoplast fusion of Lolium perenne and Lotus corniculatus for gene introgression." Diss., Lincoln University, 2007. http://hdl.handle.net/10182/301.

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Lolium perenne is one of the most important forage crops globally and in New Zealand. Lotus corniculatus is a dicotyledonous forage that contains valuable traits such as high levels of condensed tannins, increased digestibility, and high nitrogen fixing abilities. However, conventional breeding between these two forage crops is impossible due to their markedly different taxonomic origin. Protoplast fusion (somatic hybridisation) provides an opportunity for gene introgression between these two species. This thesis describes the somatic hybridisation, the regeneration and the molecular analysis

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25

Redway, F. A. "Regeneration in tissue and protoplast culture of Sainpaulia ionantha (African violet)." Thesis, University of Cambridge, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.382673.

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26

Slamet, Inez Hortense. "Protoplast culture and somatic hydridisation of Indica and Japonica rice cultivars." Thesis, University of Nottingham, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.335365.

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27

Aziz, Zaleha Biniti A. "Tissue culture of Centella asiatica : asiaticoside biosynthesis." Thesis, University of Nottingham, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.368364.

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28

Tang, Kexuan. "Studies on rice protoplast culture and transformation using an insect resistance gene." Thesis, University of Nottingham, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.294301.

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29

Barakat, M. N. R. "Protoplast and tissue culture studies for somatic hybridisation in the genus Linum." Thesis, University of Nottingham, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.304777.

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30

Gilbert, Julie E. "Interaction between mixing and density in protoplast cultures of Solanum tuberosum L." Thesis, University of Reading, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.314683.

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31

Johnson, Alexander Arthur Theodore. "Protoplast Fusion for the Production of Intermonoploid Somatic Hybrids in Cultivated Potato." Thesis, Virginia Tech, 1998. http://hdl.handle.net/10919/46514.

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Monoploid potato genotypes represent plant material that is free from the "genetic load" of lethal and severely deleterious alleles normally present in the highly heterozygous cultivated potato species. Field evaluations enabled the identification of agronomically superior monoploid potato genotypes from a population of more than 100 anther-derived monoploids. Chemical fusion and electrofusion between pairs selected from 31 superior monoploids resulted in the production of three different groups of intermonoploid somatic hybrids. The hybridity of somatic hybrid plants and callus

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32

Costa, Natalia Layane Badaró. "Improved procedures to assess plant protoplast viability: evidencing cytological and genomic damages." Universidade Federal de Viçosa, 2017. http://www.locus.ufv.br/handle/123456789/11949.

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Submitted by Reginaldo Soares de Freitas (reginaldo.freitas@ufv.br) on 2017-10-09T14:46:30Z No. of bitstreams: 1 texto completo.pdf: 4569752 bytes, checksum: 7423d0749a49ab3a18033fe827e2181d (MD5)<br>Made available in DSpace on 2017-10-09T14:46:30Z (GMT). No. of bitstreams: 1 texto completo.pdf: 4569752 bytes, checksum: 7423d0749a49ab3a18033fe827e2181d (MD5) Previous issue date: 2017-07-21<br>Conselho Nacional de Desenvolvimento Científico e Tecnológico<br>Fundação de Amparo a Pesquisa do Estado de Minas Gerais<br>Coordenação de Aperfeiçoamento de Pessoal de Nível Superior<br>Em todas as

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33

Hothersall, Joanne. "Metabolite production and molecular characterisation of interspecific Aspergilli." Thesis, University of Nottingham, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.285775.

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34

Qusus, Saba J. "Molecular Studies on Soybean Mosaic Virus-Soybean Interations." Diss., Virginia Tech, 1997. http://hdl.handle.net/10919/30328.

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In the U.S., soybean mosaic virus (SMV) is classified into seven strain groups, designated G1 to G7, based on their different responses on resistant soybean [Glycine max (L.) Merr.] cultivars. These responses are: symptomless or resistant (R), necrotic (N), and mosaic or susceptible (S). The gene-for-gene model has been proposed for SMV-soybean interactions. In the majority of cultivars, a single dominant gene, Rsv1, confers both the R and N responses. In the first part of this study, the coat protein (CP) genes of two SMV strains, G1 and G6 were isolated, cloned, and sequenced. Gene isolation

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35

Castro, Lívia Mendes de. "Isolamento, cultura de protoplastos e regeneração de plantas de laranja doce (Citrus sinensis L. Osbeck)." Universidade de São Paulo, 2010. http://www.teses.usp.br/teses/disponiveis/11/11136/tde-16032010-164451/.

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A regeneração de plantas, por organogênese ou embriogênese somática, a partir do cultivo de células e tecidos vegetais in vitro é a base para a utilização da biotecnologia no melhoramento. Realizaram-se estudos com cinco cultivares de laranja doce (Citrus sinensis L. Osbeck), Pêra, Natal, Lima Verde, Hamlin e Westin. Este trabalho objetivou a avaliação da eficiência de isolamento de protoplastos das cultivares de laranja doce; o estudo da eficiência de plaqueamento em função de cinco densidades de protoplastos e diferentes meios de cultura e avaliação da embriogênese somática em função da comp

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36

Nahar, Maksuda Anjuman. "Regeneration of plants from anther, callus and protoplast cultures of rice (Oryza sativa L.)." Thesis, University of Nottingham, 1994. http://eprints.nottingham.ac.uk/13505/.

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The work described in this thesis focuses on the development of fully reproducible systems of plant regeneration through embryogenic callus and totipotent protoplast cultures of rice (Oryza sativa L. cv. Taipei 309). The calli originated from different explants, including excised anthers, isolated microspores, mature seed-embryos, immature seed-embryos and leaf-base meristems. In the callus culture and plant regeneration studies, excised anther-derived callus produced higher numbers of green (90.5%) and albino (9.5%) plants, both haploids (81.2%) and diploids (18.8%), compared to other explant

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37

Ayeleso, Taiwo Betty. "Protoplast isolation and plant regeneration in Bambara groundnut : a platform for transient gene expression." Thesis, Cape Peninisula University of Technology, 2016. http://hdl.handle.net/20.500.11838/2003.

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Thesis (MTech (Agriculture))--Cape Peninsula University Of Technology, 2016.<br>Bambara groundnut (Vigna subterranea), a dicotyledonous plant is a legume which has a potential to contribute to food security and nutrition. Protoplasts are naked plant cells lacking cell walls. Viable protoplasts are potentially totipotent. Therefore, when given the correct stimuli, each protoplast is capable, theoretically, of regenerating a new wall and undergoing repeated mitotic division to produce daughter cells from which fertile plants may be regenerated through the tissue culture process. Protoplast syste

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38

Gokhale, D. V. "Transfer of DNA coding for cellulases from cellulomonas to bacillus subtilis by protoplast fusion." Thesis(Ph.D.), CSIR-National Chemical Laboratory, Poona, 1988. http://dspace.ncl.res.in:8080/xmlui/handle/20.500.12252/3319.

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39

Ravichandran, Vidya. "Application of molecular markers to characterize potato plants derived from anther culture and protoplast fusion." Thesis, This resource online, 1996. http://scholar.lib.vt.edu/theses/available/etd-11072008-063210/.

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40

Sinha, Debleena. "Development of an In Vitro Protoplast Culture System for Albizia Lebek (L.) Benth., an Economically Important Leguminous Tree." Thesis, University of North Texas, 1998. https://digital.library.unt.edu/ark:/67531/metadc500422/.

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An in vitro system of generating protoplasts from their callus cultures was established. The friable callus was more productive in terms of producing protoplasts than the green compact callus. The concentration of the various cell wall degrading enzymes had an effect on the viability of the protoplasts in the medium. The protoplast system developed from the experiments was stable and could be used for the transformation experiments of Albizia lebek and for other plant improvement practices.

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41

Mujahid, Hana, Feng Tan, Jian Zhang, Babi Ramesh Nallamilli, Ken Pendarvis, and Zhaohua Peng. "Nuclear proteome response to cell wall removal in rice (Oryza sativa)." BioMed Central, 2013. http://hdl.handle.net/10150/610238.

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Plant cells are routinely exposed to various pathogens and environmental stresses that cause cell wall perturbations. Little is known of the mechanisms that plant cells use to sense these disturbances and transduce corresponding signals to regulate cellular responses to maintain cell wall integrity. Previous studies in rice have shown that removal of the cell wall leads to substantial chromatin reorganization and histone modification changes concomitant with cell wall re-synthesis. But the genes and proteins that regulate these cellular responses are still largely unknown. Here we present an e

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42

Daneri, Castro Sergio Nicolas. "Germination-related cell death in the aleurone layer of malting barley." Thesis, The University of Sydney, 2016. http://hdl.handle.net/2123/15893.

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Malting of barley grain represents the most economically favourable application of this cereal. Most studies of barley grain germination have stemmed from the need to optimise the malting process, thus saving resources and obtaining a superior product. The need to optimise the malting process remains important and represents the background for the research reported in this thesis. One of the most functionally important tissues of the barley grain during germination is the aleurone layer, since it secretes most of the hydrolytic enzymes necessary for the degradation of the products stored in th

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43

Mat, Yunus Abdul Masani [Verfasser]. "Development of a protoplast-based transformation system for genetic engineering of oil palm / Abdul Masani Mat Yunus." Aachen : Hochschulbibliothek der Rheinisch-Westfälischen Technischen Hochschule Aachen, 2013. http://d-nb.info/1037936132/34.

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44

Bennett, Robert Ian. "The development of techniques for the selection of somatic hybrids of Brassica spp. produced by protoplast fusion." Thesis, University of Cambridge, 1989. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.334116.

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45

McCutchan, Jennifer Susan. "Transferring ascochyta blight resistance from Lathyrus sp. into field pea (Pisum sativum L.) via protoplast fusion (somatic hybridisation) /." Connect to thesis, 2001. http://eprints.unimelb.edu.au/archive/00000696.

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46

Lee, Sung-Ho. "Genetic transformation of rice via a protoplast-to-plant regeneration system using the rolA gene from Agrobacterium rhizogenes." Thesis, University of Nottingham, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.307761.

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47

Jelodar, Nadali Babaeian. "Protoplast culture and somatic hybridisation of cultivated rice, Oryza sativa, and the wild relative of rice, Porteresia coarctata." Thesis, University of Nottingham, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.321371.

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48

Taylor, Thomas E. "Inheritance of competencies for leaf disc regeneration, anther culture, and protoplast culture in Solanum phureja and correlations among them." Thesis, This resource online, 1991. http://scholar.lib.vt.edu/theses/available/etd-10242009-020252/.

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49

Le, Minh Phuong. "Biotransfer of selected risk metals into plants and their accumulation and distribution in plant organs." Doctoral thesis, Česká zemědělská univerzita v Praze, 2016. http://www.nusl.cz/ntk/nusl-259725.

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Contamination of soils with heavy metals is one of the serious environmental problems threatening human being. Heavy metals are considered as the special hazard of soil pollutants because of the adverse effects on the plant growth, the amount, activity of useful microorganisms in soils and the quality of food. Regard to the persistent and toxicity, the heavy metals are toxic when we consider different kinds of pollutants in soils. In the soil, zinc (Zn), cadmium (Cd), lead (Pb) and mercury (Hg) toxicities frequently occur than the other metals because of their precipitation and sorption by the

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50

WANG, MIN-XING, and 王敏行. "Observation on the suspension cell and protoplast cultures and wall regeneration from protoplasts of daucus carota L." Thesis, 1988. http://ndltd.ncl.edu.tw/handle/54521976442766643631.

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