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Dissertations / Theses on the topic 'Protoplasts'

Author: Grafiati

Published: 4 June 2021

Last updated: 25 July 2025

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1

Lynch, Paul Thomas. "The uptake of fungal protoplasts by plant protoplasts." Thesis, University of Liverpool, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.328131.

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2

Mullins, P. J. "Protoplasts from Phytophthora." Thesis, University of Liverpool, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.381366.

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3

Lawrence, W. A. "Microinjection of tobacco protoplasts." Thesis, University of East Anglia, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.372559.

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4

Attree, S. M. "Properties of Pteridium protoplasts." Thesis, University of Manchester, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.378009.

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5

Sheard, J. P. "Glucose uptake by pea mesophyll protoplasts." Thesis, University of East Anglia, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.235210.

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6

Hansen, Christine S. "Construction of galactose assimilating, carotenoid producing yeasts by protoplast fusion." Thesis, University of British Columbia, 1988. http://hdl.handle.net/2429/27935.

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Protoplasts were prepared from two yeast strains P. rhodozyma (ATCC 24202) and K. fragilis (ATCC 8455). Protoplasts prepared from P. rhodozyma were facilitated by prior growth of the cells in a media containing S-(2-aminoethyl)-L-cysteine. Protoplasts from these two yeast genera were fused either by the use of electrofusion or polyethylene glycol treatment. Stable carotenoid producing cell lines were selected by growth at 30°C on yeast nitrogen base plus galactose. Selected single fusants display taxonomic characteristics common to both genera with a cellular morphology and a carotenoid compo

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7

Souza, Bárbara Lizandra Perini de. "Fusão de protoplastos entre Penicillium echinulatum e Trichoderma harzianum para obtenção de variabilidade visando a produção de celulases." reponame:Repositório Institucional da UCS, 2007. https://repositorio.ucs.br/handle/11338/881.

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O estudo de fungos celulolíticos tem-se mostrado relevante, tendo em vista o interesse econômico do complexo celulases, especialmente na indústria têxtil e, mais recentemente, para propósitos energéticos. No presente trabalho, a fusão de protoplastos foi utilizada para combinar genótipos de mutantes parcialmente desreprimidos para produção de celulases de Penicillium echinulatum (9A02S1B9) e richoderma harzianum (AS5CH3), utilizando a técnica do doador morto, buscando-se obter recombinantes com maior produção de celulases. Nesta estratégia, ambas as linhagens tiveram seu micélio tratado com

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8

Bourne, N. "The regeneration and transformation of Bacillus protoplasts." Thesis, Cardiff University, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.376554.

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9

Newell, Jane Marie. "Vacuole development in evacuolated oat mesophyll protoplasts." Thesis, University of Southampton, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.295919.

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10

Ward, Kenneth Glenn. "Microinjection and regeneration of tobacco and potato protoplasts." Thesis, University of Cambridge, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.303971.

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11

Doulis, Andreas G. "Antioxidant responses of pea (Pisum sativum L.) protoplasts." Diss., This resource online, 1994. http://scholar.lib.vt.edu/theses/available/etd-09192008-063125/.

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12

Kuroki, Mayra Akemi. "Desenvolvimento de uma estratégia de clonagem customizada de regiões promotoras do genoma da cana-de-açúcar." Universidade de São Paulo, 2012. http://www.teses.usp.br/teses/disponiveis/87/87131/tde-05022013-082130/.

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O objetivo deste trabalho foi desenvolver uma metodologia para identificação de regiões promotoras funcionais a partir de segmentos de um genoma qualquer. O genoma da cana-de-açúcar foi escolhido para o desenvolvimento desta estratégia na qual envolve a obtenção de fragmentos de DNA os quais foram clonados em vetores de expressão. A triagem destes fragmentos foi realizada através de biobalística e resultou no isolamento de quatro clones. Um ensaio de transformação permanente em arroz com três clones gerou 12 plantas. Foi detectada expressão do marcador GUS em calos, folhas e raízes, comprovand

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13

Hörtensteiner, Stefan. "Re-formation of vacuoles in evacuolated tobacco mesophyll protoplasts /." [S.l.] : [s.n.], 1993. http://e-collection.ethbib.ethz.ch/show?type=diss&nr=10426.

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14

De, Both Michiel Theodoor Jan. "Gene transfer by electroporation in plant protoplasts and tissues." Thesis, Imperial College London, 1990. http://hdl.handle.net/10044/1/46278.

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15

Butt, Adrian David. "Physiological and biochemical studies with rubber (Hevea brasiliensis) protoplasts." Thesis, University of Nottingham, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.257197.

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16

Sproule, Anne Carleton University Dissertation Biology. "Plant regeneration from stem cortex protoplasts of Brassica juncea." Ottawa, 1987.

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17

Crotty, Christopher M. "Two distinct outward K+ conductances are simultaneously activated in TBY-2 suspension culture protoplasts." Thesis, McGill University, 2001. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=38174.

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Two kinetically and pharmacologically distinct outward K+ conductances were found to be simultaneously activated at the plasma membrane of TBY-2 suspension culture protoplasts using the whole-cell patch-clamp technique. We used a modified Hodgkin-Huxley model to quantify the activation kinetics of the outward current. Time constants for the two conductances derived from the model differed in magnitude by 5--10 fold over the voltage range from -10 mV to +50 mV, allowing their classification as either fast or slow. Deactivation kinetics were better fit by two exponential terms rather than one, y

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18

Foreman, Julia Louise. "Genetic and molecular analysis of root hair initiation and tip growth in Arabidopsis thaliana." Thesis, University of East Anglia, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.247111.

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19

Lamb, Mary Patricia. "Osmolality tolerance and ion channels in protoplasts of entomophthoralean fungi." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk2/tape16/PQDD_0006/MQ34198.pdf.

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20

Spice, Nicola Jane. "Evaluating the use of fungal protoplasts to investigate fungicide action." Thesis, Nottingham Trent University, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.341273.

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21

Curtis, Nicola. "Protoplasts as tools for the genetic study of Agraricus bisporus." Thesis, University of Nottingham, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.335201.

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22

George, Alexander Christopher Stephen. "Magnetofection of tobacco protoplasts: a novel mechanism for plant transformation." Thesis, George, Alexander Christopher Stephen (2018) Magnetofection of tobacco protoplasts: a novel mechanism for plant transformation. Honours thesis, Murdoch University, 2018. https://researchrepository.murdoch.edu.au/id/eprint/49707/.

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To help meet the challenges of climate change and a growing global population, it has never been more critical that the yields of agricultural crops are increased: new breeding technologies such as genetic engineering can play a major role in achieving increased food production. Despite several decades of study there are still limitations to plant transformation technologies. These include transformation methods based on vector-mediated introduction of gene constructs using Agrobacterium, or direct gene transfer methods such as particle bombardment or transformation of protoplasts using polyet

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23

Saka, Kamel. "REGENERATION OF COTTON (GOSSYPIUM HIRSUTUM L.) CALLUS PROTOPLASTS TO MACROCALLI." Thesis, The University of Arizona, 1985. http://hdl.handle.net/10150/275376.

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24

Sinha, Debleena. "Development of an In Vitro Protoplast Culture System for Albizia Lebek (L.) Benth., an Economically Important Leguminous Tree." Thesis, University of North Texas, 1998. https://digital.library.unt.edu/ark:/67531/metadc500422/.

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An in vitro system of generating protoplasts from their callus cultures was established. The friable callus was more productive in terms of producing protoplasts than the green compact callus. The concentration of the various cell wall degrading enzymes had an effect on the viability of the protoplasts in the medium. The protoplast system developed from the experiments was stable and could be used for the transformation experiments of Albizia lebek and for other plant improvement practices.

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25

Yokoya, Kazutomo. "Studies on the regeneration of intergeneric somatic hybrid plants of roses." Thesis, University of East London, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.360915.

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26

Stanworth, Marie Helen. "Plasma membrane ATPase of Phytophthora cactorum." Thesis, University of the West of England, Bristol, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.284886.

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27

Butler, Declan M. "Isolation and culture of protoplasts and tissues of Laminaria spp. (Phaeophyta)." Thesis, University of Leeds, 1989. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.237096.

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28

Sinha, Anupam. "Studies on the isolation and culture of Lupin (Lupinus albus) protoplasts." Thesis, University of Reading, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.297700.

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29

Al-Forkan, Mohammad. "Agrobacterium-mediated transformation of Indica rice (Oryza sativa L.)." Thesis, University of Nottingham, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.342480.

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30

Browne, H. M. "Protoplasts and their application to the study of genetics in Lipomyces starkeyi." Thesis, University of Nottingham, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.235343.

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31

Bayley, C. C. R. "An assessment of the consequences of fusion of tobacco and alfalfa protoplasts." Thesis, University of Nottingham, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.376160.

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32

Matthews, Derek. "The use of protoplasts in the regeneration and genetic manipulation of rose." Thesis, University of East London, 1993. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.385004.

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33

Jackson, R. T. "Isolation of plasma membranes from leaf protoplasts : A search for definitive markers." Thesis, University of Southampton, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.373954.

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34

Slomka, Marek Jozef. "Studies of tomato golden mosaic virus in plants, protoplasts and tissue culture." Thesis, Imperial College London, 1987. http://hdl.handle.net/10044/1/46616.

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35

Edwards, K. J. "Studies of homologous recombination between plasmid and chromosomal DNA in plant protoplasts." Thesis, University of Leicester, 1990. http://hdl.handle.net/2381/35209.

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A vector, called pWreckl, was constructed for the direct DNA transformation of N. tabacum protoplasts. This vector was shown to confer kanamycin resistance to transformed callus. Using derivatives of this plasmid containing various reporter genes, a protocol for direct DNA transformation by PEG-mediated uptake was established. However, all attempts to obtain transformed callus by electroporation were unsuccessful. The CAB6 gene from N. tabacum was identified as a suitable candidate for gene targeting and was further manipulated to produce constructs in pWreckl which were used in gene targeting

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36

Collings, Alan. "Cellulase and related enzyme activity in protoplasts and mycelium of filamentous fungi." Thesis, Sheffield Hallam University, 1986. http://shura.shu.ac.uk/19494/.

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Production of cellulase, pectinase and xylanase was investigated using culture filtrates from selected mesophilic (Aspergillus niger, Penicillium funiculosum, Penicillium janthinellum, Penicillium ochrochloron, Trichoderma viride), thermotolerant (Aspergillus fumigatus) and thermophilic (Geosmithia emersonii, formerly Talaromyces emersonii; the perfect stage of Penicillium emersonii) fungi. The fungi were grown on Acetobacter xylinum cellulose pellicles and soluble carboxymethyl cellulose and their enzyme complements were characterised under various growth conditions. The factors affecting the

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37

Penmetsa, Ramachandra V. "Factors influencing transient gene expression in electroporated tall fescue (Festuca arundinacea Schreb.) protoplasts." Thesis, This resource online, 1992. http://scholar.lib.vt.edu/theses/available/etd-09052009-040406/.

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38

Abram, Beverley Louise. "Responses of Nicotiana glauca guard cell protoplasts to changes in the culture environment." Thesis, Lancaster University, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.314238.

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39

Elshihy, O. M. "In vitro culture of tissues, cells and protoplasts of cotton (Gossypium barbadense L.)." Thesis, University of Southampton, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.374060.

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40

Starling, Anthony Patrick. "The uptake of heavy metals by protoplasts and whole cells of filamentous fungi." Thesis, Keele University, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.293925.

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41

Valles, K. L. M. "The use lipophilic ions to measure intracellular membrane potentials in intact wheat protoplasts." Thesis, University of Sussex, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.373919.

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42

Daniels, Alison. "Microscopic and computer analysis of ultrastructural changes accompanying isolation and manipulation of tobacco protoplasts." Thesis, University of Nottingham, 1989. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.278755.

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43

Harker, C. L. "The detection of virus coded proteins in cauliflower mosaic virus infected plants and protoplasts." Thesis, Bucks New University, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.376417.

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44

Yeung, Elaine Yee-Ling. "Functional roles of group vii erfs through transient transformation of protoplasts in Arabidopsis and rice." Thesis, University of California, Riverside, 2013. http://pqdtopen.proquest.com/#viewpdf?dispub=1547838.

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<p> Environmental stresses involving flooding and the accompanying cellular low oxygen (hypoxia) constitute a major limitation to agricultural production worldwide. These stresses hinder the plant's ability to maintain energy production and growth. Yet, previous studies have shown that certain genes of group VII ethylene responsive factor (ERF) family of transcription factors play a functional role in the survival of submergence and low oxygen stresses in <i>Oryza sativa</i>(rice) and <i>Arabidopsis thaliana</i>. This study utilized a transient gene transfection assay of protoplasts to charact

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45

Yan-Xiu, Zhao. "The isolation, culture and genetic manipulation of protoplasts from salt and drought tolerant leguminous plants." Thesis, Coventry University, 1993. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.332788.

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46

Shacklock, Philip S. "The involvement of second messengers in the phytochrome-mediated swelling of etiolated wheat leaf protoplasts." Thesis, University of Edinburgh, 1993. http://hdl.handle.net/1842/14380.

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Although a considerable amount of circumstantial evidence exists which indicates the involvement of Ca<SUP>2</SUP>&43 in the regulation of phytochrome-mediated responses (Bossen <i>et al</i>., 1988; Viner <i>et al</i>., 1988; Roux, 1986), the exact nature of this involvement has remained unclear. Etiolated wheat leaf protoplasts swell in response to red light (Bossen <i>et al</i>., 1988) by a mechanism which is phytochrome-mediated and is related to leaf growth and unrolling (Zhou <i>et al</i>., 1990). In this study fluorescent Ca<SUP>2</SUP>+ indicators have been used in conjunction with Lase

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47

Abdullah, R. B. "The development of a reproducible system for efficient plant regeneration from rice (Oryza sativa L.) protoplasts." Thesis, University of Nottingham, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.381060.

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48

Woskow, Steven A. "Effect of Proteolytic Enzymes on Transfection and Transformation of Streptococcus lactis Protoplasts and Transformation of Streptococcus cremoris." DigitalCommons@USU, 1987. https://digitalcommons.usu.edu/etd/5357.

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The effect of proteolytic enzymes on the transformation and transfection of Streptococcus lactis LM2301 protoplasts was examined in an attempt to eliminate the variability observed. By using both chymotrypsin and mutanolysin to form protoplasts followed by spread plating, consistent frequencies of 104 to 105 transformants per μg of pGB301 DNA, and 105 transfectants per μg of c2 bacteriophage DNA where achieved. Optimum transformation and transfection frequencies were obtained when 16 h cultures were treated simultaneously with 25 U/ml mutanolysin and 1.25 U/ml chymotrypsin for 15 min. Trypsin

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49

Gomez, Luengo Rodolfo Gustavo. "Proteins and serological relationships of maize mosaic virus isolates and replication of the virus in Maize (Zea Mays L.) protoplasts /." The Ohio State University, 1987. http://rave.ohiolink.edu/etdc/view?acc_num=osu1487327695621001.

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50

Carvalho, Dayse Cristina de. "Fusão de protoplastos visando a reconstrução da laranja azeda." Universidade de São Paulo, 2006. http://www.teses.usp.br/teses/disponiveis/11/11136/tde-12012007-160405/.

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O objetivo deste trabalho foi aplicar a técnica de fusão química de protoplastos para desenvolver híbridos somáticos interespecíficos entre tangerinas (Citrus reticulata) e toranjas (Citrus grandis), visando a obtenção de porta-enxertos semelhantes à laranja azeda Citrus aurantium). Como fonte de protoplastos foram utilizadas suspensões celulares embriogênicas de tangelo \'Page\' (C. reticulata x C. paradisi) e tangor \'Murcote\' (C. reticulata x C. sinensis) e folhas jovens de seedlings de toranjas \'Lau Tau\' e \'Ogami\' (Citrus grandis). Após a fusão os protoplastos foram cultivados sob aus

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