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Dissertations / Theses on the topic 'Pseudomonas chlororaphis'

Author: Grafiati

Published: 4 June 2021

Last updated: 26 July 2025

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1

Selin, Carrie Lynn. "Regulatory Mechanisms Underlying Biological Control Activity of Pseudomonas chlororaphis PA23." FEMS Microbial Ecology, 2010. http://hdl.handle.net/1993/9144.

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Biological control is an intriguing alternative to the use of chemical pesticides as it represents a safer, more environmentally friendly approach to managing plant pathogens. Pseudomonas chlororaphis strain PA23 was isolated from soybean root tips and it was found to be an excellent antagonist of sclerotinia stem rot. Our studies have shown that pyrrolnitrin (PRN) is the key metabolite required for S. sclerotiorum inhibition, while phenazine (PHZ) is important for biofilm establishment. For this reason, research efforts were directed towards elucidating the mechanisms governing PA23-mediate

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2

Pinho, Dora Raquel da Silva. "Isolamento e caracterização de proteínas envolvidas na via desnitrificante em Pseudomonas Chlororaphis." Doctoral thesis, Universidade do Porto. Reitoria, 2002. http://hdl.handle.net/10216/9632.

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Dissertação de Doutoramento em Química apresentada à Faculdade de Ciências da Universidade do Porto<br>O crescimento das estirpes DSM 50135 e 50083T de Pseudomonas chlororaphis em condições microaeróbicas na presença de nitrato produziu células com expressão abundante de metaloproteínas. Da estirpe DSM 50135, que se revelou um bom sistema para expressão das proteínas envolvidas na desnitrificação, isolou-se uma azurina (Az626), uma redutase do nitrito contendo cobre (CuNiR) e uma redutase membranar do nitrato (NaR). A indução da desnitrificação na estirpe DSM 50083T revelou-se mais problemátic

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3

Pinho, Dora Raquel da Silva. "Isolamento e caracterização de proteínas envolvidas na via desnitrificante em Pseudomonas Chlororaphis." Tese, Universidade do Porto. Reitoria, 2002. http://hdl.handle.net/10216/9632.

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Dissertação de Doutoramento em Química apresentada à Faculdade de Ciências da Universidade do Porto<br>O crescimento das estirpes DSM 50135 e 50083T de Pseudomonas chlororaphis em condições microaeróbicas na presença de nitrato produziu células com expressão abundante de metaloproteínas. Da estirpe DSM 50135, que se revelou um bom sistema para expressão das proteínas envolvidas na desnitrificação, isolou-se uma azurina (Az626), uma redutase do nitrito contendo cobre (CuNiR) e uma redutase membranar do nitrato (NaR). A indução da desnitrificação na estirpe DSM 50083T revelou-se mais problemátic

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4

Nandi, Munmun. "Biocontrol agents Pseudomonas brassicacearum DF41 & Pseudomonas chlororaphis PA23: Investigation of fungal suppression and defense against Caenorhabditis elegans." PLoS One, 2015. http://hdl.handle.net/1993/31674.

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The success of biocontrol bacteria is often restrained due to their low persistence in the rhizosphere and fluctuations in expression of antagonistic compounds. In the first part of this thesis the ability of the biocontrol agents (BCAs) Pseudomonas brassicacearum DF41 and Pseudomonas chlororaphis PA23 to resist grazing by the bacterivorous nematode Caenorhabditis elegans was investigated. We found that both BCAs are capable of killing the nematodes through exposure to toxic metabolites. We discovered that in addition to HCN, pyrrolnitrin (PRN) is a potent nematicide produced by PA23. Unique f

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5

Manuel, Jerrylynn Laguras. "An Investigation of the Impact of the Stringent Response on the Growth Inhibition of Sclerotinia sclerotiorum by Biocontrol Pseudomonads Pseudomonas sp. DF41 and Pseudomonas chlororaphis PA23." American Society for Microbiology, 2011. http://hdl.handle.net/1993/4791.

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The stringent response (SR) is a global regulatory mechanism that allows bacteria to survive starvation. The plant surface is one environment where a fluctuation in nutrient availability is experienced. Because both Pseudomonas sp. DF41 and Pseudomonas chlororaphis PA23 are able to protect canola from the fungal pathogen Sclerotinia sclerotiorum when applied as a foliar spray, we sought to investigate the impact of this response on the antifungal activities of these two biocontrol strains. The SR exerts its effects on gene transcription through production of the alarmone(p)ppGpp. Metabol

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6

Maddula, V. S. R. Krishna. "Quorum Sensing and Phenazines are Involved in Biofilm Formation by Pseudomonas Chlororaphis (aureofaciens) Strain 30-84." Diss., The University of Arizona, 2008. http://hdl.handle.net/10150/193917.

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Pseudomonas chlororaphis (aureofaciens) 30-84 is a biocontrol bacterium effective against take-all disease of wheat. Phenazine (PZ) production by strain 30-84 is the primary mechanism responsible for pathogen inhibition and the rhizosphere persistence of 30-84. The PhzR/PhzI system of strain 30-84 directly regulates PZ production and mutations in this QS system are defective in biofilm formation. Genetic complementation or direct addition of AHL signal restored biofilm formation to a phzI mutant. Mutations in PZ biosynthesis were equally defective in biofilm formation. Addition of PZ or genet

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7

Schmidt-Eisenlohr, Heike Barbara. "Pseudomonas chlororaphis (pJP4) als effizienter Rhizosphäre-Kolonisierer: Untersuchung fitness-relevanter Faktoren und Etablierung einer in situ-Detektionsmethode." Diss., lmu, 2002. http://nbn-resolving.de/urn:nbn:de:bvb:19-4902.

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8

Mulesky, Melinda Anne. "Rhizosphere competence, antibiotic and siderophore biosynthesis in Pseudomonas chlororaphis: implications for the biological control of cotton seedling disease pathogens." Diss., Virginia Tech, 1995. http://hdl.handle.net/10919/40235.

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9

Lee, Seungjun. "Improving Lettuce Productivity while Suppressing Biofilm Growth and Comparing Bacterial Profiles of Root Area and Nutrient Solutions in Windowfarm Systems." The Ohio State University, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=osu1398988586.

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10

Ajouz, Sakhr. "Estimation du potentiel de résistance de Botrytis cinerea à des biofongicides." Phd thesis, Université d'Avignon, 2009. http://tel.archives-ouvertes.fr/tel-00453646.

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La pourriture grise, causée par le champignon Botrytis cinerea, est l'une des principales maladies aériennes fongiques sur diverses cultures d'importance agronomique. La diversité génétique de B. cinerea est très forte et la capacité rapide d'adaptation de ce champignon à une pression sélective est également avérée. Ce champignon est ainsi capable de développer des résistances à une grande variété de composés fongicides de synthèse ou d'origine naturelle. Des méthodes alternatives de lutte ont de ce fait été développées ces dernières années : divers agents de lutte biologique (ALB) présentant

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11

Chan, Jason Hok Shun. "Functional investigation of a transcriptional regulator ptrA from Pseudomonas chlororaphis PA23." 2012. http://hdl.handle.net/1993/8895.

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Pseudomonas chlororaphis PA23 is a promising biological control candidate against Sclerotinia sclerotiorum, a fungal pathogen that causes stem rot in canola. A library of transposon mutants was previously created to understand the molecular mechanisms underlying the antifungal capabilities of PA23. A novel LysR-type transcriptional regulator, called PtrA, was identified as a key global regulator involved in secondary metabolite production. The function of PtrA at the molecular level was investigated in this thesis. Solubility problems encountered during the purification of PtrA redirected

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12

Serafim, Bruno Filipe Rodrigues. "Production of antibiotics and biopolymer by the bacterium Pseudomonas chlororaphis using glycerol." Master's thesis, 2019. http://hdl.handle.net/10362/101312.

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Members of the Pseudomonas genus are known for their ability to produce multiple secondary metabolites, including bioactive metabolites, such as antibiotics. Pseudomonas chlororaphis subsp. aurantiaca DSM 19603 is a non-pathogenic bacterium widely used as plant growth-promoting rhizobacteria that has been reported to be able to produce three products of interest, namely, antibiotics (phenazines) and two biopolymers, medium-chain-length polyhydroxyalkanoates (mcl-PHA) and exopolysaccharides (EPS). Phenazines are heterocyclic nitrogenous compounds that can act as antibiotic, antiparasitic or ev

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13

Poritsanos, Nicole Joanna. "Molecular mechanisms involved in secondary metabolite production and biocontrol of Pseudomonas chlororaphis PA23." Thesis, 2006. http://hdl.handle.net/1993/192.

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ABSTRACT Sclerotinia sclerotiorum is a ubiquitous ascomycetous fungal pathogen that causes disease in over 400 crop species, specifically in soybean and canola plants, where stem rot is the most common disease symptom. Pseudomonas chlororaphis PA23 was previously isolated from the rhizosphere of soybean and has demonstrated excellent antifungal activity against S. sclerotiorum in vitro, greenhouse and field experiments. To elucidate the molecular mechanisms involved in PA23 biocontrol, random mutagenesis experiments were initiated. Several mutants were isolated that could be divided into t

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14

Reimer, Lori. "Pseudomonas chlororaphis PA23 biocontrol of Sclerotinia sclerotiorum on canola: understanding populations and enhancing inoculation." 2016. http://hdl.handle.net/1993/31890.

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Pseudomonas chlororaphis strain PA23 has demonstrated biocontrol of Sclerotinia sclerotiorum (Lib.) de Bary, a fungal pathogen of canola (Brassica napus L.). The objectives of this research were two-fold: to optimize PA23 phyllosphere biocontrol and to investigate PA23’s influence in the rhizosphere. PA23 demonstrated longevity when inoculated on B. napus under greenhouse conditions. Carbon source differentially effected growth rate and antifungal metabolite production of PA23 in culture. Carbon source did not have a significant effect on in vivo biocontrol. PA23 demonstrated biocontrol abili

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15

Sopher, Coralie. "High temperature predisposition of sweet pepper to Pythium root rot and its remediation by Pseudomonas chlororaphis." Thesis, 2012. http://hdl.handle.net/10214/3605.

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Pythium root rot caused by Pythium aphanidermatum, a destructive disease of sweet pepper and other hydroponic crops, is characterized by root browning (necrosis) and reduces growth of roots and shoots. Serious losses in crop productivity are common, in part for lack of adequate control measures. Severe root rot has been previously associated with episodes of high temperature, but whether this is due to high temperature effects on the host, the pathogen, or their interaction remains unclear. To clarify these relationships, and to provide a basis for predicting rapid increases in root rot, quant

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16

Duke, Kelly. "Global changes in Brassica napus gene activity in response to Sclerotinia sclerotiorum and the biocontrol agent Pseudomonas chlororaphis PA23." 2016. http://hdl.handle.net/1993/31771.

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The biological control agent Pseudomonas chlororaphis PA23 is effective at protecting Brassica napus (canola) from the necrotrophic fungus Sclerotinia sclerotiorum via direct antagonism. Despite the growing importance of biocontrol bacteria in protecting crop plants from fungal pathogens, little is known about how the host plant responds to bacterial priming on the leaf surface and certainly nothing about global changes in gene activity in the presence and absence of S. sclerotiorum. PA23 priming of mature canola plants reduced the number of lesion-forming petals by 90%. Global RNA sequencing

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17

Schmidt-Eisenlohr, Heike [Verfasser]. "Pseudomonas chlororaphis (pJP4) als effizienter Rhizosphäre-Kolonisierer : Untersuchung fitness-relevanter Faktoren und Etablierung einer In-situ-Detektionsmethode / vorgelegt von Heike Schmidt-Eisenlohr." 2002. http://d-nb.info/965860728/34.

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18

Klaponski, Natasha. "Functional characterization of two divergently transcribed genes: ptrA, encoding a LysR-type transcriptional regulator, and scd, encoding a short-chain dehydrogenase in Pseudomonas chlororaphis PA23." 2014. http://hdl.handle.net/1993/23436.

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Pseudomonas chlororaphis PA23 inhibits several root pathogens in both the greenhouse and field. A LysR-type transcriptional regulator (LTTR) called PtrA (Pseudomonas transcriptional regulator A) that is essential for Sclerotinia sclerotiorum antifungal activity was discovered through transposon mutagenesis. P. chlororaphis PA23 produces the antibiotics phenazine 1-carboxylic acid, 2-hydroxyphenazine and pyrrolnitrin, and several additional products that contribute to biocontrol. Phenotypic assays and proteomic analysis have revealed that production of these secondary metabolites are markedly r

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19

Meneses, Liane Pereira de. "Evaluation of sub-, supercritical fluids as solvents for extraction and impregnation of polyhydroxyalkanoates." Master's thesis, 2017. http://hdl.handle.net/10362/25626.

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Polyhydroxyalkanoates (PHA) are a class of biobased and biodegradable polymers with potential application on different areas, depending on their composition and specific properties that range from thermoplastics to elastomers. However, PHA development is still limited by several factors, including its extraction procedures that rely on organic solvents, such as chloroform. On the other hand, it is important to develop applications of the polymers that further increase their commercial value. This work included two parts: (1) the use of hot compressed water (HCW) as an eco-friendly solvent for

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