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Journal articles on the topic 'Pseudomonas fluorescens biochemical tests'

Author: Grafiati

Published: 3 June 2025

Last updated: 19 July 2025

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1

Ibrar Wali, Syeda Asma Bano, Sobia Nisa, et al. "Isolation and Identification of Bacillus Subtilis and Pseudomonas Fluorescens from Wheat Rhizosphere and Their Use as Biocontrol Agents." Indus Journal of Bioscience Research 2, no. 2 (2024): 918–31. https://doi.org/10.70749/ijbr.v2i02.295.

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Some bacteria may be used as biocontrol agents against fungal pathogens. Biocontrol agents are environment friendly and cost effective for controlling different plant pathogens. Fungal plant pathogens cause detrimental effects on plants causing diseases and yield loss. The bacterial strains Pseudomonas fluorescens and Bacillus subtilis live abundantly in rhizospheric soil and have antagonistic activity against other organisms. The objective of present study was to isolate and identify the Pseudomonas fluorescens and Bacillus subtilis from rhizospheric soil of Triticum aestivum and their use as

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Sh. H. Kamil, R. F. ALjasani, and H. I. ALShammari. "ISOLATION, IDENTEFECATION AND EFFECINCY OF PSEUDOMONAS FLUORESCENS BACTERIA TO TERMITE MICROCEROTERMIS DIVERSUS." IRAQI JOURNAL OF AGRICULTURAL SCIENCES 54, no. 6 (2023): 1583–93. http://dx.doi.org/10.36103/ijas.v54i6.1859.

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This study was conducted to isolate the bacteria Pseudomonase fluorescens from the termite, locust, and American cockroach in the Iraqi environment and to diagnose it based on morphological, biochemical, and molecular diagnosis using the polymerase chain reaction (PCR), as well as test its pathogenicity and efficacy to termites under laboratory conditions. The results of morphological , biochemical, and molecular diagnosis using polymerase chain reaction (PCR) tests showed the isolated bacterial isolates are similar to P. fluorescens .The results of efficiency of different isolates of P. fluor

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Tasić, Srđan, and Aleksandar Janjić. "PSEUDOMONAS FLUORESCENS IN SHEEP MILK GREEK YOGHURT FROM VLASINA – A BIOCHEMICAL CHARACTERIZATION." KNOWLEDGE - International Journal 54, no. 3 (2022): 421–24. http://dx.doi.org/10.35120/kij5403421t.

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Pseudomonas fluorescens is an aerobic, rod-shaped, non-sporulating gram-negative bacteria, mostlyfound in soil, decaying organic matter and feces. This species contaminates the milk mainly through animal feeddust, silage, utensils and polluted water. In addition to casein digestion, this psychotropic and lipolytic speciesgenerates butyric and caproic acids by fermentation of milk fat, which release a strong unpleasant odor and give arancid and bitter taste to dairy products. In this study we tested 48 hours old sheep's sour milk, produced in ahousehold in Vlasina, southeastern Serbia, using tr

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Vela, Ana I., María C. Gutiérrez, Enevold Falsen, et al. "Pseudomonas simiae sp. nov., isolated from clinical specimens from monkeys (Callithrix geoffroyi)." International Journal of Systematic and Evolutionary Microbiology 56, no. 11 (2006): 2671–76. http://dx.doi.org/10.1099/ijs.0.64378-0.

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An unusual Gram-negative, catalase- and oxidase-positive, rod-shaped bacterium isolated from different clinical samples from two monkeys (Callithrix geoffroyi) was characterized by phenotypic and molecular genetic methods. The micro-organism was tentatively identified as a Pseudomonas species on the basis of the results of cellular morphological and biochemical tests. Fatty acid studies confirmed this generic placement and comparative 16S rRNA gene sequencing studies demonstrated that the unknown isolates were phylogenetically closely related to each other (100 % sequence similarity) and were

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Mehnaz, Samina, Brian Weselowski, Faheem Aftab, Sadaf Zahid, George Lazarovits, and Javed Iqbal. "Isolation, characterization, and effect of fluorescent pseudomonads on micropropagated sugarcane." Canadian Journal of Microbiology 55, no. 8 (2009): 1007–11. http://dx.doi.org/10.1139/w09-050.

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In this study, we report on the isolation, identification, and characterization of seven fluorescent pseudomonads isolated from the roots, shoots, and rhizosphere soil of sugarcane and their impacts on the growth of sugarcane plantlets. 16S rRNA gene sequence of five isolates showed close homology with Pseudomonas putida , one with Pseudomonas graminis , and one with Pseudomonas fluorescens . Physiological and biochemical characterizations were determined using API50CH and QTS24 identification kits. The isolates were also subjected to tests for various known growth promoting properties includi

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Hsueh, Po-Ren, Lee-Jene Teng, Hui-Ju Pan, et al. "Outbreak of Pseudomonas fluorescensBacteremia among Oncology Patients." Journal of Clinical Microbiology 36, no. 10 (1998): 2914–17. http://dx.doi.org/10.1128/jcm.36.10.2914-2917.1998.

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From 7 to 24 March 1997, four patients developed Pseudomonas fluorescens bacteremia at the hospital; one on the oncology ward and the other three in the chemotherapy room. These patients all had underlying malignancies and had the Port-A-Cath (Smiths Industries Medical Systems, Deltec, Inc., St. Paul, Minn.) implants. Three patients had primary bacteremia, and one had Port-A-Cath-related infection. None of these patients had received a blood transfusion before the episodes of bacteremia. All patients recovered: two received antimicrobial agents with in vitro activity against the isolates, and

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Sivagamasundari, U., and A. Gandhi. "Enhancement of Vegetative Parameters of Brinjal in Proplates by the Application of Bacterial Endophytes - Azospirillum brasilense and Pseudomonas fluorescens." International Letters of Natural Sciences 27 (October 2014): 14–18. http://dx.doi.org/10.18052/www.scipress.com/ilns.27.14.

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An experiment was carried out with endophytic fixing bacteria Azospirillum brasilense and Pseudomonas fluorescens isolated from brinjal, in different combinations with inorganic fertilizers by seed inoculation of brinjal to observe preliminary vegetative growth at 15th and 30th day and pigment contents in vegetable nursery bed (proplates). A total number of 28 endophytic bacteria isolated from brinjal from three localities (Annamalai University, Karaikal and Putthur). Further the isolates were subjected to various biochemical tests for their species level identification and nitrogen fixing abi

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Sivagamasundari, U., and A. Gandhi. "Enhancement of Vegetative Parameters of Brinjal in Proplates by the Application of Bacterial Endophytes - <i>Azospirillum brasilense</i> and<i> Pseudomonas fluorescens</i>." International Letters of Natural Sciences 27 (October 15, 2014): 14–18. http://dx.doi.org/10.56431/p-bq77ed.

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An experiment was carried out with endophytic fixing bacteria Azospirillum brasilense and Pseudomonas fluorescens isolated from brinjal, in different combinations with inorganic fertilizers by seed inoculation of brinjal to observe preliminary vegetative growth at 15th and 30th day and pigment contents in vegetable nursery bed (proplates). A total number of 28 endophytic bacteria isolated from brinjal from three localities (Annamalai University, Karaikal and Putthur). Further the isolates were subjected to various biochemical tests for their species level identification and nitrogen fixing abi

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Smith-Grenier, L. L., and A. Adkins. "Isolation and characterization of soil microorganisms capable of utilizing the herbicide diclofop-methyl as a sole source of carbon and energy." Canadian Journal of Microbiology 42, no. 3 (1996): 221–26. http://dx.doi.org/10.1139/m96-033.

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Six nonfermentative Gram-negative bacilli were isolated from Manitoban soils after enrichment with diclofopmethyl. Microscopic examination and physiological and biochemical tests have identified the organisms as Sphingomonas paucimobilis, Acinetobacter baumannii, Chryseomonas luteola, Pseudomonas aureofaciens, Pseudomonas cepacia, and Pseudomonas fluorescens. Growth curve studies showed that each of the isolates was able to grow in minimal medium with diclofop-methyl as the sole source of carbon and energy. Chemical analysis confirmed that all of the added diclofop-methyl (1.5 μg ∙ mL−1) had b

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Mustafa, Sameer Midhat, and Maher Abed Suha. "Isolation and identification of pathogenic species of the genus Pseudomonas and study of antibiotic resistance." GSC Biological and Pharmaceutical Sciences 23, no. 1 (2023): 087–98. https://doi.org/10.5281/zenodo.7924953.

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The bacteria <em>Pseudomonas aeruginosa</em> were isolated and diagnosed and compared with <em>Pseudomonas fluorescens</em> for the period between September 2021 and March 2022, in Ghazi Hariri Hospital, Yarmouk and the Medical City.) to investigate Pseudomonas aeruginosa and <em>Pseudomonas fluorescens</em> and other species study antibiotic resistance. (90) samples were collected using a cotton swab and a sterile container. The diagnosis was made based on the culture characteristics and biochemical tests, after which the diagnosis was confirmed at the qualitativ

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Mustafa Sameer Midhat and Suha Maher Abed. "Isolation and identification of pathogenic species of the genus Pseudomonas and study of antibiotic resistance." GSC Biological and Pharmaceutical Sciences 23, no. 1 (2023): 087–98. http://dx.doi.org/10.30574/gscbps.2023.23.1.0144.

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The bacteria Pseudomonas aeruginosa were isolated and diagnosed and compared with Pseudomonas fluorescens for the period between September 2021 and March 2022, in Ghazi Hariri Hospital, Yarmouk and the Medical City.) to investigate Pseudomonas aeruginosa and Pseudomonas fluorescens and other species study antibiotic resistance. (90) samples were collected using a cotton swab and a sterile container. The diagnosis was made based on the culture characteristics and biochemical tests, after which the diagnosis was confirmed at the qualitative level using the phytic technique. Carry out a sensitivi

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LIMA, Ana Beatriz Mori, Lara Stefânia Netto de Oliveira LEÃO-VASCONCELOS, Dayane de Melo COSTA, et al. "Pseudomonas spp. ISOLATED FROM THE ORAL CAVITY OF HEALTHCARE WORKERS FROM AN ONCOLOGY HOSPITAL IN MIDWESTERN BRAZIL." Revista do Instituto de Medicina Tropical de São Paulo 57, no. 6 (2015): 513–14. http://dx.doi.org/10.1590/s0036-46652015000600009.

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This cross-sectional study, performed in an oncology hospital in Goiania, aimed to characterize the prevalence of oral colonization and antimicrobial susceptibility of Pseudomonas spp. isolated from the saliva of healthcare workers. Microorganisms were subjected to biochemical tests, susceptibility profile, and phenotypic detection. Of 76 participants colonized with Gram negative bacilli, 12 (15.8%) harbored Pseudomonas spp. Of all isolates, P. aeruginosa (75.0%), P. stutzeri (16.7%), and P. fluorescens (8.3%), were resistant to cefoxitin, and therefore likely to be AmpC producers. The results

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Karnwal, Arun, and Aradhana Dohroo. "Effect of maize root exudates on indole-3-acetic acid production by rice endophytic bacteria under influence of L-tryptophan." F1000Research 7 (January 25, 2018): 112. http://dx.doi.org/10.12688/f1000research.13644.1.

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Background: It is assumed that plant growth regulators produced by beneficial bacterial species could also influence plant growth. IAA is a major plant growth regulator responsible for stimulation of plant growth. There are several microorganisms which are naturally responsible for L- tryptophan metabolism. Methods: In total, 56 indigenous morphologically distinct isolates from rice roots were selected and subsequently characterized with biochemical tests, 16S rRNA sequencing and plant growth promoting activities. Pseudomonas fluorescens RE1 (GenBank: MF102882.1) and RE17 (GenBank: MF103672.1)

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Chauret, C., L. Stover, C. Volk, T. S. Dykstra, G. A. Gagnon, and R. C. Andrews. "Effect of disinfectants on microbial ecology in model distribution systems." Journal of Water and Health 3, no. 4 (2005): 359–69. http://dx.doi.org/10.2166/wh.2005.050.

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This research was conducted to assess the impact of various disinfectants on bacterial water quality within model distribution systems (i.e. annular reactors). After colonization with non-disinfected water, annular reactors were treated with relatively low doses of chlorine (0.4 mg/l), chlorine dioxide (0.15 mg/l), or chloramines (0.9 mg/l). Under the tested conditions, bacterial inactivation varied as a function of disinfectant type (ranking by efficiency per mg of oxidant: ClO2 &gt; Cl2 &gt; ClNH2) and sample type (bulk water vs. biofilm). Depending on the disinfectant, the log inact

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Rangel, Ana Beatriz Ferreira, Jean Thiago Alves Soares, Mariana Maciel Pereira, Bruna Rachel de Britto Peçanha, Leonardo Emanuel de Oliveira Costa, and Janaína dos Santos Nascimento. "Inhibition of food-related bacteria by antibacterial substances produced by Pseudomonas sp. strains isolated from pasteurized milk." Brazilian Journal of Food Technology 16, no. 4 (2013): 326–33. http://dx.doi.org/10.1590/s1981-67232013005000039.

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In this work, the production of antimicrobial substances by strains of Pseudomonas sp. isolated from pasteurized milk and their potential action against food-related bacteria were investigated. Samples of pasteurized milk were purchased from arbitrarily chosen commercial establishments in the city of Rio de Janeiro, Brazil. Of the four samples analyzed, three presented several typical colonies of Pseudomonas. About 100 colonies were chosen and subjected to biochemical tests for confirmation of their identity. Eighteen strains of the Pseudomonas genus were identified and submitted to tests for

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Kheira, Benyoub, Kacem Mourad, and Kaid-Harche Meriem. "Detection of Pseudomonas syringae pv. Savastanoi, causal agent of olive tuberculosis in two regions of Western Algeria (Ain TÃ©mouchent and Sig)." South Asian Journal of Experimental Biology 9, no. 2 (2019): 64–71. http://dx.doi.org/10.38150/sajeb.9(2).p64-71.

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The present study on olive tuberculosis commenced by isolating bacteria of the genus Pseudomonas from the soils and necrosis of collected olive trees. A total of 180 samples were used in this study: (100) rhizospheric soil samples: (60) samples at the region of Ain Témouchent and (40) at the region of Sig in western of Algeria. In total, (80) galls were collected (40) at branch level and (40) galls at olive tree leaf (level). The isolates were identified by microbiological (macroscopic and microscopic examination), physiological (growth in the presence of Salt (NaCl), growth at different pH va

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Nigam, Rashmi, A. K. Sharma, and Joginder Singh. "Screening of Pseudomonas sp. isolated from rhizosphere of pea plant as plant growth promoter and biocontrol agent." International Journal of Agricultural Invention 1, no. 02 (2016): 138–44. http://dx.doi.org/10.46492/ijai/2016.1.2.3.

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Plant growth promoting rhizobacteria (PGPR) are a heterogeneous group of bacteria that are found in the rhizosphere and rhizoplane which can improve plant growth. Pseudomonas spp. is one of the most promising groups of PGPR which can control plant pathogenic microbes in the soil. In this study, an attempt was made to isolate Pseudomonas spp., a potent PGPR in the rhizosphere. Through appropriate microbiological and biochemical methods, the study demonstrated the presence of fluorescent and nonfluorescent Pseudomonads in the rhizosphere of pea. 12 different strains of Pseudomonas were isolated

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Milijasevic-Marcic, Svetlana, Biljana Todorovic, Milos Stepanovic, et al. "Monitoring of bacterial diseases of Agaricus bisporus in Serbia." Pesticidi i fitomedicina 31, no. 1-2 (2016): 29–35. http://dx.doi.org/10.2298/pif1602029m.

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Monitoring of button mushroom bacterial diseases was conducted to estimate the presence and identity of mycopathogenic bacteria and to determine the predominant bacterial pathogen in Serbia. Samples were collected from mushroom farms during 2006- 2010 and also from fresh markets during 2014-2015. The collected samples showed either symptoms of brown blotch or different degrees of brown discoloration on caps and stalks of Agaricus bisporus resembling bacterial infection. The presence of bacterial droplets on gills was not recorded. The isolated bacteria were Gram-negative and fluorescent on Kin

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Agbo, Ejiofor C., Obiora R. Ejinaka, Obiorah E. Okechukwu, Obeta M. Uchejeso, Jwanse I. Rinpan, and Ifeoma E. Lote-Nwaru. "Molecular Identification of Biofilm-forming Pseudomonas aeruginosa Isolates from Noncritical Surfaces of a Tertiary Healthcare Center in Abia State, Southeast Nigeria." Journal of Biochemistry, Microbiology and Biotechnology 10, no. 1 (2022): 25–30. http://dx.doi.org/10.54987/jobimb.v10i1.659.

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The presence of biofilm-forming Pseudomonas aeruginosa on noncritical surfaces in a hospital has been attributed to healthcare-associated infections (HAIs) leading to multidrug-resistant (MDR) infections. This study is aimed at identifying the molecular characteristics of Biofilm forming Pseudomonas aeruginosa found on non-critical surfaces in a hospital environment. Samples were collected using the swab technique from different noncritical surfaces surrounding hospitalized patients in different wards namely sphygmomanometers, thermometers, bed rails etc. Bacterial analysis was performed by us

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Cai, Xianghai, Siqi Chen, Hong Yang, et al. "Biodegradation of waste greases and biochemical properties of a novel lipase from Pseudomonas synxantha PS1." Canadian Journal of Microbiology 62, no. 7 (2016): 588–99. http://dx.doi.org/10.1139/cjm-2015-0641.

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A lipase-producing bacterial strain was isolated from oil-well-produced water in Shengli oilfield (Shandong province, China) and was identified as Pseudomonas synxantha by 16S rDNA sequence analysis (named Pseudomonas synxantha PS1). Strain PS1 showed a maximum lipase activity of 10.8 U/mL after culturing for 48 h at 30 °C, with lactose (4 g/L) as carbon source, tryptone (8 g/L) as nitrogen source, olive oil (0.5%, v/v) as inductor, and the initial pH 8.0. Meanwhile, the lipase gene from P. synxantha PS1 was cloned and expressed in Escherichia coli BL21 with the vector pET28a. The novel gene (

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Adel Qasim, Doaa. "Isolation and identification of some types of histamine-producing bacteria from shrimp in local markets." Sumer 1 8, CSS 1 (2023): 1–10. http://dx.doi.org/10.21931/rb/css/s2023.08.01.47.

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Different genera and species of bacteria were isolated from shrimp, which were brought from the Al-Faw area in the Basra governorate, and these isolates were diagnosed after purification through studying their phenotypic characteristics and conducting API confirmation, biochemical tests, motility examination and staining with gram stain, in addition to using different types of diagnostic tools bacteria. Twenty-five bacterial isolates of genera and species of bacteria were isolated and diagnosed from shrimp. Morganella morganii sub. Morganii,, Klebsiella pneumonia sub. Pneumonia, Proteus marina

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Oloninefa, Stephen Dare, J. E. Aisoni, V. K. Fadayomi, and O. Oghenemaro. "Phytochemical Screening and Antibacterial Activity of Whole Plant Crude Extracts of Euphorbia hirta Linn against Some Clinical Isolates." Sahel Journal of Life Sciences FUDMA 2, no. 2 (2024): 1–7. http://dx.doi.org/10.33003/sajols-2024-0202-01.

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The problem of bacteria resistance coupled with microorganisms being implicated as causative agents of many infectious diseases has led to the search for substances (phytochemicals) that can be used to stop the activity of these microorganisms and to reduce or eliminate their effect on the human population and to also curb the menace of bacteria resistance. This study aimed at carrying out phytochemical screening and antibacterial activity of whole plant crude extracts of Euphorbia hirta Linn against some clinical isolates. The selected clinical isolates (Escherichia coli, Pseudomonas fluoresc

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Nande, Mas’ud Yusuf. "Isolation and characterization of lipase producing bacteria in hydrocarbon contaminated sites in Kano Metropolis." Dutse Journal of Pure and Applied Sciences 10, no. 2c (2024): 410–21. http://dx.doi.org/10.4314/dujopas.v10i2c.37.

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Lipase (Triacylglycerol hydrolases EC 3.1.1.3) is an enzyme capable of hydrolyzing lipids into fatty acids and glycerol. It possesses numerous industrial applications such as pharmaceuticals, food, detergents, paper and pulp, agrochemicals, biosurfactants and bioremediation, etc. Many attempts have been made to isolate lipase producing microorganisms since this enzyme is used in many biotechnological processes. This study was aimed at isolation and characterization of lipase producing bacteria in hydrocarbon contaminated soil sites in Kano metropolis. Bacterial isolation was done through seria

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Molan, Y., and Y. Ibrahim. "First Report of Tomato (Lycopersicon esculentum) Pith Necrosis Caused by Pseudomonas fluorescens and P. corrugata in the Kingdom of Saudi Arabia." Plant Disease 91, no. 1 (2007): 110. http://dx.doi.org/10.1094/pd-91-0110b.

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From 2002 to 2004, tomato (Lycopersicon esculentum) plants with external stem lesions, adventitious roots, and necrotic pith that was hollowed or chambered were received by the Clinical Lab of the Plant Protection Department from eight greenhouses in the Riyadh, Abha, and El-Kharj regions of Saudi Arabia. Bacteria were isolated on nutrient agar or King's medium B (KMB) from the stems of tomato plants cv. Red Gold, the cultivar most commonly grown in greenhouses. Gram-negative, rod-shaped bacteria were consistently isolated from stems with symptoms of pith necrosis. They were identified as Pseu

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Wimalajeewa, DLS, ND Hallam, AC Hayward, and TV Price. "The etiology of head rot disease of broccoli." Australian Journal of Agricultural Research 38, no. 4 (1987): 735. http://dx.doi.org/10.1071/ar9870735.

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Transmission and scanning electron microscope studies of broccoli florets affected by head rot, at various stages of disease development, strongly indicated a bacterial etiology for the disease. Nevertheless, the different species of bacteria isolated from diseased heads, using standard techniques, failed to reproduce symptoms in pathogenicity tests conducted in the glasshouse and in the field. However, a modified isolation technique, using broccoli heads showing incipient watersoaking symptoms, yielded a fluorescent pseudomonad which reproduced disease symptoms readily in glasshouse and field

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AJIBOYE, Elizabeth Adeyinka, Hikmat Omolara SULAYMAN, and Abdullahi Taiwo AJAO. "Bioremediation of Spent Engine Oil on Selected Contaminated Soils within Ilorin Metropolis." Advanced Journal of Graduate Research 8, no. 1 (2020): 91–104. http://dx.doi.org/10.21467/ajgr.8.1.91-104.

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The research aimed to investigate the bioremediation of spent engine oil on selected contaminated soils within Ilorin metropolis. To achieve this, soil samples were collected from three (3) mechanic workshops along Taiwo axis within the metropolis. The soil samples were then subjected to bioremediation using the land-farming approach. The physicochemical parameters of the soil samples before and after bioremediation were analyzed using standard methods. Bacteria were isolated using standard procedures and identified using biochemical tests and molecular methods. Results for the physicochemical

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Selvi, G. S. Anantha, D. Chelladurai, V. Pattu Kumar, and M. Navin Chandran. "Bacterial Flacherie in Bombyx mori (L): Isolation and Identification of Pathogenic Organism." UTTAR PRADESH JOURNAL OF ZOOLOGY 44, no. 14 (2023): 52–61. http://dx.doi.org/10.56557/upjoz/2023/v44i143558.

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The major restraint in silk production is silkworm diseases caused mainly by Bacterial flacherie. Bacillus subtilis, Streptococcus pneumoniae, Staphylococcus aureus, Pseudomonas fluorescence and Bacillus cereus are the different bacteria were identified and isolated from the infected worm by means of colony morphology, staining property and biochemical tests. The main objectives of this study to isolate and identify the pathogen which causes flacherie disease in silkworm (B. mori).

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Monir, Md Shirajum, Shuvho Chakra Borty, Nazneen Bagum, Md Khalilur Rahman, Md Alimul Islam, and Yahia Mahmud. "Identification of pathogenic bacteria isolated from diseased stinging catfish, Shing (Heteropneustes fossilis) cultured in greater Mymensingh, Bangladesh." Asian-Australasian Journal of Bioscience and Biotechnology 1, no. 1 (2016): 116–24. http://dx.doi.org/10.3329/aajbb.v1i1.61544.

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Stinging catfish, Shing (Heteropneustes fossilis) is been cultured in Bangladesh which is one of the most popular and highly valuable fish species. A total of 120 diseased H. fossilis were collected from twenty five fish farms in greater Mymensingh region, Bangladesh between November 2013 to February 2014. Remarkable clinical symptoms of the affected fishes were loss of equilibrium and, erosions at the bases of fins and tail, hemorrhages and skin lesions on body surface, profuse mucous secretion and congestion along with enlargement of most of the internal organs. Three different kinds of sele

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Ayyal, Nagham Mohammed, Zainab Abdulzahra Abbas, Abdulkarim Jafar Karim, et al. "Bacterial isolation from internal organs of rats (Rattus rattus) captured in Baghdad city of Iraq." Veterinary World 12, no. 1 (2019): 119–25. http://dx.doi.org/10.14202/vetworld.2019.119-125.

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Aim: Rats are accused in disseminating many zoonotic diseases. This study aimed to isolate and identify bacteria from internal organs of rats captured in Baghdad City, Iraq. Materials and Methods: A total of 120 black rats (R. rattus) were trapped from different areas in Baghdad city. Rats were kept in individual plastic cages for 3 h before euthanizing. Deep pharyngeal swab, intestinal content, urine, and pieces of the liver and spleen, lung, kidney, and brain were obtained aseptically. The specimens were inoculated into peptone water and incubated at 37°C for 24 h for enrichment. A loopful o

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Cruz, Darío, Rodrigo Cisneros, Ángel Benítez, et al. "Gram-Negative Bacteria from Organic and Conventional Agriculture in the Hydrographic Basin of Loja: Quality or Pathogen Reservoir?" Agronomy 11, no. 11 (2021): 2362. http://dx.doi.org/10.3390/agronomy11112362.

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Organic and conventional agriculture are vital for the development of human society; however, the use of contaminated water and the inappropriate use of organic chemical fertilizers can lead to an increase in the microbial load (potentially pathogenic) of the normal microbiota of the agricultural soil. In this context, the aim of our study was to isolate Gram-negative bacteria from the superficial soil layer and irrigation water of agricultural areas (11 organic farms and nine conventional farms) and consider their potential ecological and health risk importance. Through culture isolation usin

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Hanudin, Hanudin, and Indijarto Budi Rahardjo. "KARAKTERISTIK PSEUDOMONAS VIRIDIFLAVA: PENYEBAB PENYAKIT BUSUK LUNAK DAN EVALUASI VIRULENSINYA PADA KLON ANGGREK PHALAENOPSIS." Jurnal Hama dan Penyakit Tumbuhan Tropika 11, no. 2 (2011): 185–93. http://dx.doi.org/10.23960/j.hptt.211185-193.

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The objectives of this research were to determine the typical symptoms and isolates chararteristics of Pseudomonas viridiflava as the causal agent of soft rot bacterial disease and to find out orchid Phalaenopsis clones resistant to the disease. The scope of this study included exploration and isolation of the pathogens, pathogenicity, biochemical and LOPAT tests, also screening the Phalaenopsis F1 clones resistant to the pathogen. The results showed that isolates Vd-6, Ph-7 and Ph-18 obtained from Vanda (in Bandung Nursery) and Phalaenopsis orchids respectively, in glass house of Indonesian O

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Khabbaz, Salah Eddin, and Pervaiz A. Abbasi. "Isolation, characterization, and formulation of antagonistic bacteria for the management of seedlings damping-off and root rot disease of cucumber." Canadian Journal of Microbiology 60, no. 1 (2014): 25–33. http://dx.doi.org/10.1139/cjm-2013-0675.

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Antagonistic bacteria are common soil inhabitants with potential to be developed into biofungicides for the management of seedling damping-off, root rot, and other soil-borne diseases of various crops. In this study, antagonistic bacteria were isolated from a commercial potato field and screened for their growth inhibition of fungal and oomycete pathogens in laboratory tests. The biocontrol potential of the 3 most effective antagonistic bacteria from the in vitro tests was evaluated against seedling damping-off and root rot of cucumber caused by Pythium ultimum. Based on phenotypic characteris

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Tanvir, Mustafizur Rahman, Md Shahjalal Sagor, Rafsan Abir, et al. "Chemical and Microbiological Quality of Municipal Supply Water in Dhaka South Area, Bangladesh." European Journal of Biology and Biotechnology 5, no. 3 (2024): 21–28. http://dx.doi.org/10.24018/ejbio.2024.5.3.502.

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This project aimed to find out the microbiological quality of municipal supply water in Dhaka South area and if the current regime of microbiological quality testing can determine the risk to public health from supply water satisfactorily. Water samples were collected from six different points of the water distribution network of Dhaka’s south locality, starting from the source (Shitalakhyariver, Narayanganj) to Jagannath University, Dhaka. The dominant taxonomic units were isolated using standard culture techniques, followed by biochemical identification, VITEK2 identification, and biofilm-fo

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Saha, Raina, Vaishnavi Kodidhala, P. Bhaumik, A. Debnath, and P. M. Bhattacharya. "In-vitro & In-vivo Studies on Antimicrobial and Plant Growth Promoting Efficacy of Fluorescent Pseudomonads." Journal of Advances in Biology & Biotechnology 27, no. 10 (2024): 932–50. http://dx.doi.org/10.9734/jabb/2024/v27i101516.

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Aims: Excessive use of chemical fungicides cause environmental pollution and its residual effects are deleterious for human and animal consumption. So, application of biological control agents in IDM is an ecofriendly complement to manage the diseases as well as to protect the environment. PGPRs are free living microorganisms which have antimicrobial properties as well as exert beneficial effects to plant growth. Study Design: This present study has been designed to identify potential indigenous fluorescent Pseudomonads strains from different crop ecosystems under Eastern Gangetic plains of We

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Bouaichi, .A, Benkirane R., A.AMEUR, S.SADIK, Benbouazza A., and H. Achbani E. "Characterization of Pantoea agglomerans Strains Isolated from Olive Knot disease in Morocco." Biolife 3, no. 2 (2022): 528–34. https://doi.org/10.5281/zenodo.7270238.

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<strong>ABSTRACT</strong> On the base of prospecting conducted during the agriculture season 2012-2013, the results revealed that the olive knot disease caused by <em>Pseudomonas</em> <em>savastanoi</em> pv.<em>savastanoi</em> was observed in different olive groves in Morocco. The disease incidence varies with location and cultivar. However, high disease incidence was reported in Taounate, Ouazzane and Meknes. The biochemical and the molecular tests were revealed the presence of saprophytic bacteria; <em>Pantoea agglomerans</em>, <em>Enterobacter</em> sp., <em>Pseudomonas </em>sp., associated

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Dinata, Gallyndra Fatkhu, Luqman Qurata Aini, and Restu Rizkyta Kusuma. "Identification and Characterization of Antagonistic Bacteria from Coffee Plant Litter." Agrotechnology Research Journal 5, no. 1 (2021): 32–37. http://dx.doi.org/10.20961/agrotechresj.v5i1.49716.

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Nowadays, the use of antagonistic bacteria is widely applied in agriculture and used as biological control. A previous study which is yet to be identified based on physiological and biochemical characterizations discovered these organisms present in coffee plant litter could control fusarium wilt disease. Therefore, this study is a follow-up analysis that aims to identify coffee litter antagonistic bacteria with the ability to control fusarium wilt disease. The investigation techniques used were biochemical tests comprising of endospore staining, yellow colony growth test on media, bacterial p

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Végh, A., M. Hevesi, Zs Némethy, and L. Palkovics. "First Report of Bacterial Leaf Spot of Basil Caused by Pseudomonas viridiflava in Hungary." Plant Disease 96, no. 1 (2012): 141. http://dx.doi.org/10.1094/pdis-09-11-0726.

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In April 2011, typical bacterial spot symptoms were observed on sweet basil plantlets (Ocimum basilicum L.) in a supermarket in Budapest, Hungary. Affected plants had dark brown-to-black lesions on the cotyledons. Spots on the leaves were first water soaked and then became necrotic and progressed inward from the margins. Symptoms were similar to those reported by Little et al. (3) on basil affected by Pseudomonas viridiflava. Bacteria consistently isolated from leaf lesions formed mucoid colonies with a green fluorescent pigment on King's B medium. Strains were gram negative. In LOPAT (levan-o

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Koike, Steven T., Jeri D. Barak, Diana M. Henderson, and Robert L. Gilbertson. "Bacterial Blight of Leek: A New Disease in California Caused by Pseudomonas syringae." Plant Disease 83, no. 2 (1999): 165–70. http://dx.doi.org/10.1094/pdis.1999.83.2.165.

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During 1996 and 1997, a new and damaging disease of leek (Allium porrum) was observed on greenhouse-produced transplants and field-grown plants in California. Symptoms were water-soaked lesions at leaf tips, which eventually expanded down the length of the leaf and resulted in brown, elongated, stripe-like lesions with yellow margins. Diseased leaves eventually wilted. A blue fluorescent pseudomonad was consistently recovered from lesions, and biochemical and physiological tests indicated that it was Pseudomonas syringae. Pathogenicity tests established that representative strains of this P. s

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Admassie, Mesele, Yitbarek Woldehawariat, and Tesfaye Alemu. "In Vitro Evaluation of Extracellular Enzyme Activity and Its Biocontrol Efficacy of Bacterial Isolates from Pepper Plants for the Management of Phytophthora capsici." BioMed Research International 2022 (September 26, 2022): 1–13. http://dx.doi.org/10.1155/2022/6778352.

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Phytophthora capsici is one of the most devastating fungal pathogens, causing severe diseases that lead to economic loss in the pepper industry. As a result of the infections, the chemical approach is becoming more popular. Biological control, on the other hand, is better suited to controlling fungal pathogens. The biological control approach significantly reduces the problems associated with chemical applications while restoring natural environmental balance. As a result, the overall findings indicate that certain bacterial isolates play a beneficial role in lytic enzyme production and biocon

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Oloninefa, S. D, Abalaka, M. E, Daniyan, S. Y, and Mann, A. "Comparisons Between Different Crude Extract Yields Of Whole Plant Of Momordica Charantia And Their Antibacterial Susceptibility Against Selected Clinical Isolates." UMYU Journal of Microbiology Research (UJMR) 3, no. 1 (2018): 61–70. http://dx.doi.org/10.47430/ujmr.1831.010.

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Medicinal plants such as Momordica charantia contained different phytochemicals which have antibacterial activity against Escherichia coli, Staphylococcus aureus, Pseudomonas fluorescens, Salmonella typhimurium and Klebsiella pneumoniae. In this study, four solvents namely n- hexane, ethyl acetate, methanol and aqueous were used for the reflux extraction of whole plant of M. charantia successively and exhaustively. The phytochemicals and antibacterial susceptibility of the crude extracts against selected clinical isolates were determined at different concentrations of 40 mg/ml, 60 mg/ml, 80 mg

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Benlioglu, K., Ü. Özyilmaz, and D. Ertan. "First Report of Bacterial Blight Caused by Pseudomonas syringae pv. pisi on Pea in Turkey." Plant Disease 94, no. 7 (2010): 923. http://dx.doi.org/10.1094/pdis-94-7-0923a.

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In April of 2009, leaf blight symptoms were observed on field peas (Pisum sativum L.) grown in Söke, Torbali, and Ödemis counties in the Aegean Region of Turkey. Field inspections revealed disease incidence as high as 45% and the disease was found in 13 commercial fields. Initial symptoms consisted of small, dark green, water-soaked lesions on leaves, stipules, and stems near ground level. Lesions often enlarged and coalesced and turned chocolate brown with a water-soaked margin. Stem infections usually coalesced and girdled the stem spreading upward to stipules and leaflets forming a fan-like

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ALMOMANI, FOUAD A., IHAB MANASREH, YOUSEF M. ABU-ZAITOON, and ABDEL RAHMAN AL TAWAHA. "Diversity, pathogenicity and biocontrol efficacy of Pseudomonas syringae isolated from plants in northern Jordan." Romanian Biotechnological Letters 27, no. 1/2022 (2022): 3264–69. http://dx.doi.org/10.25083/rbl/27.1/3264-3269.

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Diversity, pathogenicity, and biocontrol efficacy of Pseudomonas syringae strains isolated from a wide range of habitats in Jordan were investigated. Thirty-five infected plant samples (vegetable leaves and woody plant twig) were randomly collected. King's B agar medium was implemented and 124 colonies of P. syringae candidates were selected. Phenotypic, biochemical and pathogenicity tests were further carried out to confirm the identity of P. syringae isolates. The frequency of fluorescent isolates varied between 11- 50 %. Even though all isolates were shown to induce chlorosis of tobacco lea

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Djitro, Noel, Rebecca Roach, Rachel Mann, Brendan Rodoni, and Cherie Gambley. "Characterization of Pseudomonas syringae Isolated from Systemic Infection of Zucchini in Australia." Plant Disease 106, no. 2 (2022): 541–48. http://dx.doi.org/10.1094/pdis-05-21-1039-re.

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Zucchini plants with symptoms including twisted petioles, necrotic leaves, crown rot, and internal fruit rot were found in Bundaberg, Australia, at a commercial field for the first time during late autumn 2016, resulting in direct yield losses of 70 to 80%. Three Pseudomonas syringae strains isolated from symptomatic leaf (KL004-k1), crown (77-4C), and fruit (KFR003-1) were characterized and their pathogenicity evaluated on pumpkin, rockmelon, squash, and zucchini. Biochemical assays showed typical results for P. syringae. The three isolates differed, however, in that two produced fluorescent

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Rado, R., N. Rakotoarimanga, FR Fienena, et al. "Biocontrol of potato wilt by selective rhizospheric and endophytic bacteria associated with potato plant." African Journal of Food, Agriculture, Nutrition and Development 15, no. 68 (2015): 9762–76. http://dx.doi.org/10.18697/ajfand.68.15005.

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Ralstonia solanacearum is the causative agent of wilt disease in plants, which constitutes a severe problem to agricultural crops, particularly for potato production in Madagascar. The present study focuses on the isolation, in vitro and in vivo assays of potential rhizospheric and endophytic bacteria associated with healthy potato plant, capable to inhibit the growth of Ralstonia solanacearum for controlling potato bacterial wilt. A total of 77 bacteria strains were isolated from six soil rhizospheric samples and six vegetal material samples of healthy potatoes in the district of Antsirabe II

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Martín-Sanz, A., J. L. Palomo, and C. Caminero. "First Report of Bacterial Blight Caused by Pseudomonas syringae pv. syringae on Common Vetch in Spain." Plant Disease 93, no. 12 (2009): 1348. http://dx.doi.org/10.1094/pdis-93-12-1348b.

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Common vetch (Vicia sativa L.) is an important legume crop used for livestock feed in the Mediterranean Area. This crop has an important role for sustainable agriculture in dryland rotations in Spain, where the Castilla y León Region is the major production area. During the springs of 2007 and 2008, necrotic lesions on stems, leaves, and flowers were observed in five different common vetch plots around Medina de Rioseco (Castilla y León). Four of the plots were sown with cv. Buza. No information was available about the cultivar in the fifth plot. In many cases, lesions had expanded into the st

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Balaž, J., R. Iličić, S. Maširević, D. Jošić, and S. Kojić. "First Report of Pseudomonas syringae pv. syringae Causing Bacterial Leaf Spots of Oil Pumpkin (Cucurbita pepo) in Serbia." Plant Disease 98, no. 5 (2014): 684. http://dx.doi.org/10.1094/pdis-07-13-0714-pdn.

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Oil pumpkin (Cucurbita pepo L.) is commonly used for oil production, mainly in central and eastern Europe (1). In Serbia, it grows only in the north (Vojvodina Province), up to 1,500 ha. In June 2008, typical bacterial spot symptoms (dark green, water-soaked, transparent and greasy spots with yellow margins) were observed for the first time, cultivated at the experimental fields near Backi Petrovac. Since then, bacterial spots were regularly observed on oil pumpkin in the beginning of the growing seasons and during rainy weather, with disease incidence ranging from 5 to 20%. Bacteria isolated

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Sahin, F., R. Kotan, and M. F. Dönmez. "First Report of Bacterial Blight of Mulberries Caused by Pseudomonas syringae pv. mori in the Eastern Anatolia Region of Turkey." Plant Disease 83, no. 12 (1999): 1176. http://dx.doi.org/10.1094/pdis.1999.83.12.1176b.

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During spring 1999, a severe bacterial blight disease was observed on white mulberry (Morus alba L.) trees grown in the Erzincan, Erzurum, and Artvin provinces in the eastern Anatolia region of Turkey. Initial symptoms appeared as small, water-soaked spots on leaves and shoots. The spots eventually expanded and resulted in dark brown to black, elongated, stripe-like lesions on shoots. Diseased leaves wilted at the twig tips and later died. Disease incidence was close to 100% in the regions surveyed. Fluorescent bacteria were isolated consistently from lesions on diseased leaves and shoots on K

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Journal, Baghdad Science. "Bacteriological and Cytological study For bronchial washes from lung cancer patients." Baghdad Science Journal 8, no. 1 (2011): 406–15. http://dx.doi.org/10.21123/bsj.8.1.406-415.

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The study included the collection of 75 bronchial wash samples from patients suspected to have lung cancer. These samples were subjected to a diagnostic cytological study to detect the dominant type of lung cancer. It was noticed that 33 patients proved to have a lung cancer out of 75 (44%) of these, 19 cases (57.6%)were diagnosed having Squamus cell carcinoma,7cases (21.21%) showed Adenocarcinoma ,6 cases (18.18%) were having small cell carcinoma while only one case (3.03%)was large cell carcinoma .Nearly 70% of cases were correlated with smokers .Bacteria were isolated from 53 patients in wh

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AL-Jubori, Sawsan S., Mohsn A. Ali, and Amna M. Ali. "Bacteriological and Cytological study For bronchial washes from lung cancer patients." Baghdad Science Journal 8, no. 1 (2011): 406–15. http://dx.doi.org/10.21123/bsj.2011.8.1.406-415.

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The study included the collection of 75 bronchial wash samples from patients suspected to have lung cancer. These samples were subjected to a diagnostic cytological study to detect the dominant type of lung cancer. It was noticed that 33 patients proved to have a lung cancer out of 75 (44%) of these, 19 cases (57.6%)were diagnosed having Squamus cell carcinoma,7cases (21.21%) showed Adenocarcinoma ,6 cases (18.18%) were having small cell carcinoma while only one case (3.03%)was large cell carcinoma .Nearly 70% of cases were correlated with smokers .Bacteria were isolated from 53 patients in wh

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Harighi, B. "Angular Leaf Spot of Cucumber Caused by Pseudomonas syringae pv. lachrymans in Kurdistan." Plant Disease 91, no. 6 (2007): 769. http://dx.doi.org/10.1094/pdis-91-6-0769a.

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During the spring of 2004 and 2005, angular leaf spot was observed on cucumber (Cucumis sativus L.) in some areas of Kurdistan Province in Iran for the first time. Disease incidence in affected fields was approximately 100%. Symptoms were initially small, round or irregular, water-soaked spots on leaves ranging from 3 to 5 mm in diameter. These spots were limited by the leaf veins that gave them an angular appearance. Under humid conditions, tiny, white exudates formed on the undersides of the leaves and severely infected leaves turned yellow. A fluorescent pseudomonad was consistently isolate

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