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Journal articles on the topic 'Pseudomonas fluorescens identification'

Author: Grafiati

Published: 3 June 2025

Last updated: 31 July 2025

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1

Meyer, Jean-Marie, Valérie A. Geoffroy, Nader Baida, et al. "Siderophore Typing, a Powerful Tool for the Identification of Fluorescent and Nonfluorescent Pseudomonads." Applied and Environmental Microbiology 68, no. 6 (2002): 2745–53. http://dx.doi.org/10.1128/aem.68.6.2745-2753.2002.

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ABSTRACT A total of 301 strains of fluorescent pseudomonads previously characterized by conventional phenotypic and/or genomic taxonomic methods were analyzed through siderotyping, i.e., by the isoelectrophoretic characterization of their main siderophores and pyoverdines and determination of the pyoverdine-mediated iron uptake specificity of the strains. As a general rule, strains within a well-circumscribed taxonomic group, namely the species Pseudomonas brassicacearum, Pseudomonas fuscovaginae, Pseudomonas jessenii, Pseudomonas mandelii, Pseudomonas monteilii, “Pseudomonas mosselii,” “Pseud

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2

Ibrar Wali, Syeda Asma Bano, Sobia Nisa, et al. "Isolation and Identification of Bacillus Subtilis and Pseudomonas Fluorescens from Wheat Rhizosphere and Their Use as Biocontrol Agents." Indus Journal of Bioscience Research 2, no. 2 (2024): 918–31. https://doi.org/10.70749/ijbr.v2i02.295.

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Some bacteria may be used as biocontrol agents against fungal pathogens. Biocontrol agents are environment friendly and cost effective for controlling different plant pathogens. Fungal plant pathogens cause detrimental effects on plants causing diseases and yield loss. The bacterial strains Pseudomonas fluorescens and Bacillus subtilis live abundantly in rhizospheric soil and have antagonistic activity against other organisms. The objective of present study was to isolate and identify the Pseudomonas fluorescens and Bacillus subtilis from rhizospheric soil of Triticum aestivum and their use as

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3

Dirmawati, Suskandini Ratih. "IDENTIFIKASI SURFAKTIN PADA PSEUDOMONAS FLUORESCENS ST1 PENGENDALI EFEKTIF PENYAKIT PUSTUL KEDELAI." Jurnal Hama dan Penyakit Tumbuhan Tropika 9, no. 1 (2009): 54–57. http://dx.doi.org/10.23960/j.hptt.1954-57.

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Identification of surfactin in Pseudomonas fluorescens ST1 which effectively suppres soybean bacterial pustule. Identification of surfactin in Pseudomonas fluorescens ST1 filtrate was conducted in Plant Pest and Disease Laboratory, Bogor Agriculture University. The 48 hours cultured suspension of P. fluorescens ST1 with 108 CFU/ml density was centrifuged to obtain the supernatant. The supernatant was analyzed for its surfactin content by High Performance Liquid Chromatography with Colum ODS-5 and eluen acetonitril and acetat acid. The result showed that surfactin was producted by P. fluorescen

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4

Brown, Gerry, Zeayen Khan, and Ran Lifshitz. "Plant growth promoting rhizobacteria: strain identification by restriction fragment length polymorphisms." Canadian Journal of Microbiology 36, no. 4 (1990): 242–48. http://dx.doi.org/10.1139/m90-042.

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A genomic library of the Pseudomonas putida strain GR12-2 was screened to identify both genus-universal and strain-specific 8-kilobase inserts. The genus-universal clone (pAM141), in combination with the restriction enzymes EcoRI, PstI, and PvuII, was used to generate unique restriction fragment length polymorphisms for 20 related plant growth promoting rhizobacteria and seven reference strains. Strain restriction fragment length polymorphism profiles based on the genus-universal clone pAM141 allow positive identification of individual pseudomonad strains. The strain-specific clone (pAM227) cl

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5

Falih, Aysar Ihsan, and Naresh K. Y. "Isolation Identification and Charecterization of Pencillinase Producing Bacteria from Soil." SAR Journal of Pathology and Microbiology 6, no. 04 (2025): 123–45. https://doi.org/10.36346/sarjpm.2025.v06i04.001.

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Aim: To identify the penicillin resistance organisms in soil sample collected from the hospital area. Methods: Bacteria were grown on nutrient agar medium and sub cultured in nutrient broth with different concentrations of Penicillin. The resistance organisms were characterized by 16s rRNA gene sequencing and blast analysis. Results: A total of 21 cultural were identified as penicillin resistance organisms. Out of 21 cultures we have characterized four cultures and identified as Pseudomonas putida, Pseudomonas fluorescens, Comamonas sp. KBB4, Bacterium SM2-6. Conclusion: Pseudomonas putida, Ps

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6

Mustafa, Sameer Midhat, and Maher Abed Suha. "Isolation and identification of pathogenic species of the genus Pseudomonas and study of antibiotic resistance." GSC Biological and Pharmaceutical Sciences 23, no. 1 (2023): 087–98. https://doi.org/10.5281/zenodo.7924953.

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The bacteria <em>Pseudomonas aeruginosa</em> were isolated and diagnosed and compared with <em>Pseudomonas fluorescens</em> for the period between September 2021 and March 2022, in Ghazi Hariri Hospital, Yarmouk and the Medical City.) to investigate Pseudomonas aeruginosa and <em>Pseudomonas fluorescens</em> and other species study antibiotic resistance. (90) samples were collected using a cotton swab and a sterile container. The diagnosis was made based on the culture characteristics and biochemical tests, after which the diagnosis was confirmed at the qualitativ

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7

Sorathiya Shreya Kalariya, Kalpesh. "Isolation, Identification, and Biocontrol Potential of Plant Growth Promoting Rhizobacteria Pseudomonas fluorescens." International Journal of Science and Research (IJSR) 12, no. 8 (2023): 503–8. http://dx.doi.org/10.21275/sr23728111145.

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8

Mehnaz, Samina, Brian Weselowski, Faheem Aftab, Sadaf Zahid, George Lazarovits, and Javed Iqbal. "Isolation, characterization, and effect of fluorescent pseudomonads on micropropagated sugarcane." Canadian Journal of Microbiology 55, no. 8 (2009): 1007–11. http://dx.doi.org/10.1139/w09-050.

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In this study, we report on the isolation, identification, and characterization of seven fluorescent pseudomonads isolated from the roots, shoots, and rhizosphere soil of sugarcane and their impacts on the growth of sugarcane plantlets. 16S rRNA gene sequence of five isolates showed close homology with Pseudomonas putida , one with Pseudomonas graminis , and one with Pseudomonas fluorescens . Physiological and biochemical characterizations were determined using API50CH and QTS24 identification kits. The isolates were also subjected to tests for various known growth promoting properties includi

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9

Mustafa Sameer Midhat and Suha Maher Abed. "Isolation and identification of pathogenic species of the genus Pseudomonas and study of antibiotic resistance." GSC Biological and Pharmaceutical Sciences 23, no. 1 (2023): 087–98. http://dx.doi.org/10.30574/gscbps.2023.23.1.0144.

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The bacteria Pseudomonas aeruginosa were isolated and diagnosed and compared with Pseudomonas fluorescens for the period between September 2021 and March 2022, in Ghazi Hariri Hospital, Yarmouk and the Medical City.) to investigate Pseudomonas aeruginosa and Pseudomonas fluorescens and other species study antibiotic resistance. (90) samples were collected using a cotton swab and a sterile container. The diagnosis was made based on the culture characteristics and biochemical tests, after which the diagnosis was confirmed at the qualitative level using the phytic technique. Carry out a sensitivi

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10

Gobbetti, M., and J. Rossi. "Peptidase Profiles of Pseudomonas fluorescens: Identification and Properties." Journal of Dairy Science 75, no. 4 (1992): 924–34. http://dx.doi.org/10.3168/jds.s0022-0302(92)77832-1.

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11

Morimoto, Yuh, Mari Tohya, Zulipiya Aibibula, Tadashi Baba, Hiroyuki Daida, and Teruo Kirikae. "Re-identification of strains deposited as Pseudomonas aeruginosa, Pseudomonas fluorescens and Pseudomonas putida in GenBank based on whole genome sequences." International Journal of Systematic and Evolutionary Microbiology 70, no. 11 (2020): 5958–63. http://dx.doi.org/10.1099/ijsem.0.004468.

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The taxonomic classification of Pseudomonas species has been revised and updated several times. This study utilized average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) cutoff values of 95 and 70 %, respectively, to re-identify the species of strains deposited in GenBank as P. aeruginosa , P. fluorescens and P. putida . Of the 264 deposited P. aeruginosa strains, 259 were correctly identified as P. aeruginosa , but the remaining five were not. All 28 deposited P. fluorescens strains had been incorrectly identified as P. fluorescens . Four of these strains were re-identifi

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12

S., Gosh, Debnath A., Bandyopadhyay S., and Bhattacharya P. M. "Identification and Evaluation of Antagonistic Potential of Different fluorescent pseudomonads in Northern Plains of West Bengal." Environment and Ecology 42, no. 3 (2024): 1060–68. http://dx.doi.org/10.60151/envec/jrxw3511.

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To achieve future food security, reliable crop protection will be critically important. Development of biological control for soil borne disease is accepted as a safe, durable and ecofriendly alternative for chemical management strategy. Pseudomonads possess many traits like production antibiotics, lytic enzymes, siderophores, hydrogen cyanide (HCN) that make them well suited as biocontrol and growth-promoting agents. An attempt was taken to isolate and identify different fluorescent Pseudomonads and to evaluate their antagonistic ability. Twelve fluorescent Pseudomonads isolates were collecte

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13

Hsueh, Po-Ren, Lee-Jene Teng, Hui-Ju Pan, et al. "Outbreak of Pseudomonas fluorescensBacteremia among Oncology Patients." Journal of Clinical Microbiology 36, no. 10 (1998): 2914–17. http://dx.doi.org/10.1128/jcm.36.10.2914-2917.1998.

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From 7 to 24 March 1997, four patients developed Pseudomonas fluorescens bacteremia at the hospital; one on the oncology ward and the other three in the chemotherapy room. These patients all had underlying malignancies and had the Port-A-Cath (Smiths Industries Medical Systems, Deltec, Inc., St. Paul, Minn.) implants. Three patients had primary bacteremia, and one had Port-A-Cath-related infection. None of these patients had received a blood transfusion before the episodes of bacteremia. All patients recovered: two received antimicrobial agents with in vitro activity against the isolates, and

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14

Chapalain, A., G. Rossignol, O. Lesouhaitier, et al. "Comparative study of 7 fluorescent pseudomonad clinical isolates." Canadian Journal of Microbiology 54, no. 1 (2008): 19–27. http://dx.doi.org/10.1139/w07-110.

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There is some debate about the potential survival of Pseudomonas fluorescens at temperatures above 37 °C and its consequences for infectious potential, owing to the heterogeneity of clinical strains. Seven clinical strains growing at 37 °C or more were submitted for polyphasic identification; 2 were identified as Pseudomonas mosselii and 4 were precisely characterized as P. fluorescens bv. I or II. The binding indexes on glial cells of the strains identified as P. fluorescens bv. I and P. mosselii were compared with that of a reference psychrotrophic strain, P. fluorescens MF37 (bv. V). Clinic

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15

Förster-Fromme, Karin, Birgit Höschle, Christina Mack, Michael Bott, Wolfgang Armbruster, and Dieter Jendrossek. "Identification of Genes and Proteins Necessary for Catabolism of Acyclic Terpenes and Leucine/Isovalerate in Pseudomonas aeruginosa." Applied and Environmental Microbiology 72, no. 7 (2006): 4819–28. http://dx.doi.org/10.1128/aem.00853-06.

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ABSTRACT Geranyl-coenzyme A (CoA)-carboxylase (GCase; AtuC/AtuF) and methylcrotonyl-CoA-carboxylase (MCase; LiuB/LiuD) are characteristic enzymes of the catabolic pathway of acyclic terpenes (citronellol and geraniol) and of saturated methyl-branched compounds, such as leucine or isovalerate, respectively. Proteins encoded by two gene clusters (atuABCDEFGH and liuRABCDE) of Pseudomonas aeruginosa PAO1 were essential for acyclic terpene utilization (Atu) and for leucine and isovalerate utilization (Liu), respectively, as revealed by phenotype analysis of 10 insertion mutants, two-dimensional ge

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16

Hunjan, Mandeep Singh, Anjali Thakur, and Pushpinder Paul Singh. "Identification and characterization of Pseudomonas fluorescensstrains effective against Xanthomonasoryzaepv. oryzae causing bacterial blight of rice in Punjab, India." Journal of Applied and Natural Science 9, no. 1 (2017): 253–61. http://dx.doi.org/10.31018/jans.v9i1.1181.

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For the control of bacterial blight of rice caused by Xanthomonasoryzaepv. oryzae, sixty four Pseudomonas fluorescens strains were recovered from rice and wheat rhizosphere. These strains were identified on the basis of internal transcribed spacer (ITS) region. It was observed that the strains showing fluorescence in the selective media showed the amplification of the targeted P. fluorescens specific ITS region. The strains were also characterized for the production of the antibiotic 2, 4-diacetylphloroglucinol (DAPG) using phlDlocus. The characteristic 750bp region was amplified in all the DA

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17

Hearn, Elizabeth M., Jonathan J. Dennis, Murray R. Gray, and Julia M. Foght. "Identification and Characterization of the emhABC Efflux System for Polycyclic Aromatic Hydrocarbons in Pseudomonas fluorescens cLP6a." Journal of Bacteriology 185, no. 21 (2003): 6233–40. http://dx.doi.org/10.1128/jb.185.21.6233-6240.2003.

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ABSTRACT The hydrocarbon-degrading environmental isolate Pseudomonas fluorescens LP6a possesses an active efflux mechanism for the polycyclic aromatic hydrocarbons phenanthrene, anthracene, and fluoranthene but not for naphthalene or toluene. PCR was used to detect efflux pump genes belonging to the resistance-nodulation-cell division (RND) superfamily in a plasmid-cured derivative, P. fluorescens cLP6a, which is unable to metabolize hydrocarbons. One RND pump, whose gene was identified in P. fluorescens cLP6a and was designated emhB, showed homology to the multidrug and solvent efflux pumps i

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18

Gill, Santokh, Julie Belles-Isles, Gerry Brown, et al. "Identification of variability of ribosomal DNA spacer from Pseudomonas soil isolates." Canadian Journal of Microbiology 40, no. 7 (1994): 541–47. http://dx.doi.org/10.1139/m94-087.

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The polymerase chain reaction was used to amplify the spacer region located between the 16S and 23S ribosomal RNA genes of strains of Pseudomonas fluorescens and Pseudomonas putida isolated from peat bog, canola field, or arctic plants. Some of these amplified spacer regions were used to probe Southern blots of total DNA digests from the various strains of P. fluorescens and P. putida. Differences were observed in the patterns of hybridization of the various bacterial DNAs. The ribosomal DNA spacer region of four of the P. fluorescens strains examined, strains 64-3, 63-28, QP5, and R17-FP2, wa

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19

Tasić, Srđan, and Aleksandar Janjić. "PSEUDOMONAS FLUORESCENS IN SHEEP MILK GREEK YOGHURT FROM VLASINA – A BIOCHEMICAL CHARACTERIZATION." KNOWLEDGE - International Journal 54, no. 3 (2022): 421–24. http://dx.doi.org/10.35120/kij5403421t.

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Pseudomonas fluorescens is an aerobic, rod-shaped, non-sporulating gram-negative bacteria, mostlyfound in soil, decaying organic matter and feces. This species contaminates the milk mainly through animal feeddust, silage, utensils and polluted water. In addition to casein digestion, this psychotropic and lipolytic speciesgenerates butyric and caproic acids by fermentation of milk fat, which release a strong unpleasant odor and give arancid and bitter taste to dairy products. In this study we tested 48 hours old sheep's sour milk, produced in ahousehold in Vlasina, southeastern Serbia, using tr

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20

HU, LIANXIA, SHUFEI ZHANG, YULING XUE, et al. "Rapid Identification of Pseudomonas fluorescens Harboring Thermostable Alkaline Protease by Real-Time Loop-Mediated Isothermal Amplification." Journal of Food Protection 85, no. 3 (2021): 414–23. http://dx.doi.org/10.4315/jfp-21-272.

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ABSTRACT Thermostable alkaline protease (TAP) harbored by Pseudomonas fluorescens decomposes protein in milk and dairy products, leading to milk and dairy product spoilage during storage. Thus, a specific, sensitive, rapid, and simple method is required to detect TAP-harboring P. fluorescens. Two sets of primers targeting the aprX and gyrB genes of P. fluorescens were designed. The detection system and conditions were optimized, and a real-time loop-mediated isothermal amplification (real-time LAMP) method was developed for the simultaneous detection of TAP-harboring P. fluorescens in two sepa

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21

Teshome, Befekadu, Eleni Belay, Barkelegne Mengesha, Zerihun Tsegaye, Edwin K. Akley, and Awuku Frederick. "Selection and identification of Pseudomonas and Bacillus Rhizobacteria with Bioinoculant potential for Sorghum." International Journal of Science, Technology, Engineering & Mathematics 5, no. 2 (2025): 38–59. https://doi.org/10.53378/ijstem.353191.

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This study aimed to select and identify elite strains of plant growth-promoting rhizobacteria (PGPR) among phosphate-solubilizing bacteria (PSB) conserved at the Ethiopian Biodiversity Institute. In-vitro screening for plant growth-promoting traits and abiotic stress tolerance identified 12 promising PGPR isolates. These strains exhibited phosphate solubilization (index 1.8–3.2), auxin production, nitrogen fixation, and ammonia production. All isolates showed drought tolerance, and some exhibited salt tolerance (up to 10% NaCl). 16S rDNA sequencing identified Pseudomonas frederiksbergensis, Ps

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22

CHENG, CHORNG-MING, MICHAEL P. DOYLE, and JOHN B. LUCHANSKY. "Identification of Pseudomonas fluorescens Strains Isolated from Raw Pork and Chicken That Produce Siderophores Antagonistic towards Foodborne Pathogens." Journal of Food Protection 58, no. 12 (1995): 1340–44. http://dx.doi.org/10.4315/0362-028x-58.12.1340.

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Three strains of Pseudomonas fluorescens (strains 13, 43, and 52) isolated from ground pork or chicken meat exhibited broad-spectrum antimicrobial activity against both gram-positive (Listeria monocytogenes and Staphylococcus aureus) and gram-negative (Salmonella enteritidis, Escherichia coli O157:H7, Yersinia enterocolitica, and Campylobacter jejuni) foodborne pathogens, but not lactic acid bacteria (Lactobacillus and Pediococcus). Inhibition was not due to production of organic acids or other antimicrobials, but was related to the availability of iron, with the addition of ferric chloride to

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23

Sivagamasundari, U., and A. Gandhi. "Enhancement of Vegetative Parameters of Brinjal in Proplates by the Application of Bacterial Endophytes - Azospirillum brasilense and Pseudomonas fluorescens." International Letters of Natural Sciences 27 (October 2014): 14–18. http://dx.doi.org/10.18052/www.scipress.com/ilns.27.14.

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An experiment was carried out with endophytic fixing bacteria Azospirillum brasilense and Pseudomonas fluorescens isolated from brinjal, in different combinations with inorganic fertilizers by seed inoculation of brinjal to observe preliminary vegetative growth at 15th and 30th day and pigment contents in vegetable nursery bed (proplates). A total number of 28 endophytic bacteria isolated from brinjal from three localities (Annamalai University, Karaikal and Putthur). Further the isolates were subjected to various biochemical tests for their species level identification and nitrogen fixing abi

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Sivagamasundari, U., and A. Gandhi. "Enhancement of Vegetative Parameters of Brinjal in Proplates by the Application of Bacterial Endophytes - <i>Azospirillum brasilense</i> and<i> Pseudomonas fluorescens</i>." International Letters of Natural Sciences 27 (October 15, 2014): 14–18. http://dx.doi.org/10.56431/p-bq77ed.

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An experiment was carried out with endophytic fixing bacteria Azospirillum brasilense and Pseudomonas fluorescens isolated from brinjal, in different combinations with inorganic fertilizers by seed inoculation of brinjal to observe preliminary vegetative growth at 15th and 30th day and pigment contents in vegetable nursery bed (proplates). A total number of 28 endophytic bacteria isolated from brinjal from three localities (Annamalai University, Karaikal and Putthur). Further the isolates were subjected to various biochemical tests for their species level identification and nitrogen fixing abi

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25

Yao Fulgence Koffi, Moumouny Traoré, Amoa Armist Tehua, Adjoua Marthe Ehui, and Wazé Aimée Mireille Alloue-Boraud. "Reduction of fungal spoilage in Amélie variety mango (Mangifera indica L.) using biocontrol agents." GSC Biological and Pharmaceutical Sciences 29, no. 3 (2024): 165–73. https://doi.org/10.30574/gscbps.2024.29.3.0430.

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The mango is today one of the five most produced and consumed fruits in the world after the orange, the banana, the grape and the apple. It is one of the three most exported fruits from Côte d’Ivoire with banana and pineapple. However, the mango sector faces enormous post-harvest losses due to diseases and handling activities. It is in this context that the objective of this work is to reduce post-harvest losses of the Amélie variety mango, using formulations based on biocontrol agents (Pseudomonas fluorescens and Bacillus subtilis) against fungal germs spoilage. To do this, isolation and iden

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MOHAMMAD, Anwar O., Abdulkareem E. S. ALKURTANY, and Abdullah A. HASSAN. "Evaluation of API 20E system in fluorescent Pseudomonas identification from button mushroom Agaricus bisporus cultivation casing soil." Notulae Scientia Biologicae 12, no. 2 (2020): 258–63. http://dx.doi.org/10.15835/nsb12210628.

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Bacterial activity, mainly Pseudomonas spp. plays a vital role in the fruiting process of white button mushroom, hence a rapid procedure to identify these bacteria is crucial. In the current study, the validity of commercial identification system, Analytical profile index API 20E to identify Pseudomonas isolates from mushroom casing soil were assessed. Using API strips fifty bacterial isolates from a selective medium (King B medium) were examined, all isolates were belonged to the genus Pseudomonas according to API 20E identification systems. However, only 74% of Pseudomonas bacteria were iden

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27

Kůdela, V., V. Krejzar, and I. Pánková. "Pseudomonas corrugata and Pseudomonas marginalis associated with the collapse of tomato plants in rockwool slab hydroponic culture." Plant Protection Science 46, No. 1 (2010): 1–11. http://dx.doi.org/10.17221/44/2009-pps.

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Plant pathogenic species Pseudomonas corrugata and P. marginalis were detected and determined in collapsed tomato plants in rockwool slab hydroponic culture in southern Moravia, Czech Republic. Surprisingly, P. marginalis was also determined before planting in apparently healthy grafted tomato transplants grown in hydroponic culture. Moreover, non-pathogenic P. fluorescens, P. putida, P. synxantha, and Stenotrophomonas malthophilia were identified. The Biolog Identification GN2&nbsp;MicroPlate&trade; System System (Biolog, Inc., Hayward, USA) was used for identification of bacterial is

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Said, Layla Abdulhamed, Sawsan Saeed Hasan, and Saad L. Hamed. "Identification of Clinical Pseudomonas spp. by VITEK 2 Compact System and Species-specific Polymerase Chain Reaction Assay for Identification of Pseudomonas aeruginosa." International Journal of Drug Delivery Technology 10, no. 03 (2020): 383–88. http://dx.doi.org/10.25258/ijddt.10.3.14.

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The objective of this study was to isolate, identify, and diagnose Pseudomonas spp. from different clinical sources in Baghdad, Iraq. VITEK 2 compact system identification gram-negative bacteria (ID gNB) cards were used to confirm the identification. Polymerase chain reaction (PCR) technique and sequencing were used for recognition of the 16S rDNA gene, by two pairs of primers, universal primers (930 bp fragments) for recognition of Pseudomonas spp., and Pseudomonas aeruginosa specific species (PASS) primers (956 bp fragments) for differentiation of P. aeruginosa from other species. Amplified

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Li, B., G. L. Wang, Z. Y. Wu, W. Qiu, Q. M. Tang, and G. L. Xie. "First Report of Bacterial Head Rot of Broccoli Caused by Pseudomonas fluorescens in China." Plant Disease 93, no. 11 (2009): 1219. http://dx.doi.org/10.1094/pdis-93-11-1219a.

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During warm and humid periods in the winters from 2005 to 2008, head rot symptoms on broccoli (cv. Sijilv) (Brassica oleracea L. var italica Planch) were observed in commercial fields in Ningbo, Zhejiang Province, China. In agreement with the report of Cui and Harling (1), water-soaked lesions developed on the buds and then progressed into a brown-black soft rot. Longitudinal sections of the symptomatic inflorescences showed brown discoloration and rotting of the internal tissues. Broccoli production is hampered by the disease, with disease incidence ranging from 65 to 81%. Bacteria were isola

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Lim, Ju Hyoung, Ho-Gun Rhie, and Jeong Nam Kim. "Identification and Analysis of Putative Polyhydroxyalkanoate Synthase (PhaC) in Pseudomonas fluorescens." Journal of Microbiology and Biotechnology 28, no. 7 (2018): 1133–40. http://dx.doi.org/10.4014/jmb.1803.03006.

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David, Kamei, and U. Singh Archana. "Studies on the isolation, identification and enzyme activity of bioagent Pseudomonas fluorescens used for controlling brown spot disease of rice caused by Helminthosporium oryzae." World Journal of Advanced Research and Reviews 12, no. 2 (2021): 458–61. https://doi.org/10.5281/zenodo.5773299.

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In the present investigation studies was carried out ontheIsolation, Identification and Enzyme activity of bioagent <em>Pseudomonas fluorescens</em> used for controlling Brown spot disease of Rice caused by <em>Helminthosporium oryzae</em>(Breda de Haan).This is a fungal pathogen causing major disease that causes enormous losses in grain yield (upto 90%) particularly when leaf spotting phase assumes epiphytotic proportions.

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Ahn, Jung Hoon, Jae Gu Pan, and Joon Shick Rhee. "Identification of the tliDEF ABC Transporter Specific for Lipase in Pseudomonas fluorescensSIK W1." Journal of Bacteriology 181, no. 6 (1999): 1847–52. http://dx.doi.org/10.1128/jb.181.6.1847-1852.1999.

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ABSTRACT Pseudomonas fluorescens, a gram-negative psychrotrophic bacterium, secretes a thermostable lipase into the extracellular medium. In our previous study, the lipase of P. fluorescensSIK W1 was cloned and expressed in Escherichia coli, but it accumulated as inactive inclusion bodies. Amino acid sequence analysis of the lipase revealed a potential C-terminal targeting sequence recognized by the ATP-binding cassette (ABC) transporter. The genetic loci around the lipase gene were searched, and a secretory gene was identified. Nucleotide sequencing of an 8.5-kb DNA fragment revealed three co

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Rajendran, Narayanan. "Identification and Cloning of a Gene Locus Encoding Peptide Synthetase of Pseudomonas fluorescens by Two Sets of PCR Primers." Zeitschrift für Naturforschung C 54, no. 1-2 (1999): 105–9. http://dx.doi.org/10.1515/znc-1999-1-218.

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A chromosomal locus encoding biosynthetic genes for a putative peptide synthetase of Pseudomonas fluorescens was identified and cloned. To achieve this, two sets of degenerated oligonucleotide primers KAGGA:SGTTG and TGD :LGG were used in PCR. These primers were selected based on highly conserved units of known peptide synthetases involved in adenylation and thiolation regions of Bacillus subtilis. The discrete amplified bands from PCR ca. 300 bp for KAGGA:SGTTG and ca. 500 bp for TGD:LGG proved to be integral part of the genomic DNA of P. fluorescens were cloned and sequenced. Sequence alignm

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Sonawane, Avinash, Ute Klöppner, Sven Hövel, Uwe Völker, and Klaus-Heinrich Röhm. "Identification of Pseudomonas proteins coordinately induced by acidic amino acids and their amides: a two-dimensional electrophoresis study." Microbiology 149, no. 10 (2003): 2909–18. http://dx.doi.org/10.1099/mic.0.26454-0.

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The acidic amino acids (Asp, Glu) and their amides (Asn, Gln) are excellent growth substrates for many pseudomonads. This paper presents proteomics data indicating that growth of Pseudomonas fluorescens ATCC 13525 and Pseudomonas putida KT2440 on these amino acids as sole source of carbon and nitrogen leads to the induction of a defined set of proteins. Using mass spectrometry and N-terminal sequencing, a number of these proteins were identified as enzymes and transporters involved in amino acid uptake and metabolism. Most of them depended on the alternative sigma factor σ 54 for expression an

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A.Al-Fathedhal, Fadhal, and Duaa A.Al-Khafaji. "Bioactivity of Bacteria Bacillus subtilis and Pseudomonas fluorescens against Rhizoctonia solani and Fusarium solani, which causes seed rot, seedling on cucumber in greenhouses." Al-Kufa University Journal for Biology 10, no. 3 (2018): 146–63. http://dx.doi.org/10.36320/ajb/v10.i3.8107.

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The research contracts with the study of the antagonistic effect of microbe against cucumber disease caused by Rhizoctonia solani and Fusarium solani. --Bacillus subtilis and Pseudomonas fluorescens were isolated from cucumber field soil. Some of the fungi diagnosing that cause the death of cucumber seeds in the greenhouse in Najaf Governorate. The results showed identification of R. solani and F. solani was carried out using the PCR technique,with forefronts and back-fronts primers in a range of about 600 pairs to a nitrogen base (Base paris,bp). The analysis showed a new isolation of Rhizoct

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RUSSELL, SCOTT M. "A Rapid Microbiological Method for Enumeration of Pseudomonas fluorescens from Broiler Chicken Carcasses." Journal of Food Protection 60, no. 4 (1997): 385–90. http://dx.doi.org/10.4315/0362-028x-60.4.385.

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A method to selectively enumerate Pseudomonas fluorescens from fresh chicken carcasses in less than 24 h using capacitance microbiology was developed. Capacitance assays were conducted on whole-carcass rinses at 25°C using brain heart infusion broth (BHI) containing 25 μg of Irgasan per ml to obtain a detection time. The capacitance samples were spread plated on plate count agar for isolation and identification. From plates with the highest dilution, from each carcass, 4 colonies were randomly selected and identified. Seven species of bacteria including Pseudomonas fluorescens were responsible

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M. D. Makut, C. U. Agu, N. F. Okey-Ndeche, J. E. Owuna, and A. E. Nsofor. "Screening for antibacterial producing bacteria isolated from soil in Keffi, Nasarawa, State, Nigeria." GSC Biological and Pharmaceutical Sciences 22, no. 3 (2023): 225–34. http://dx.doi.org/10.30574/gscbps.2023.22.3.0110.

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Antibiotics are secondary metabolites that inhibit other competing cells to give competitive advantage to the microorganisms that produce them. This study is aimed at screening for antibacterial producing bacteria isolated from soil in Keffi. Standard microbiological methods were used in isolation and screening for antimicrobial of activity of crude extracts. Molecular identification of bacteria isolated were carried out using 16srRNA method. The antibacterial activity was carried out using agar well diffusion methods. The highest bacteria were Isolated from maize rhizosphere (90.0%) and the l

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Wiedmann, Martin, Denise Weilmeier, Sean S. Dineen, Robert Ralyea, and Kathryn J. Boor. "Molecular and Phenotypic Characterization ofPseudomonas spp. Isolated from Milk." Applied and Environmental Microbiology 66, no. 5 (2000): 2085–95. http://dx.doi.org/10.1128/aem.66.5.2085-2095.2000.

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ABSTRACT Putative Pseudomonas spp. isolated predominantly from raw and processed milk were characterized by automated ribotyping and by biochemical reactions. Isolates were biochemically profiled using the Biolog system and API 20 NE and by determining the production of proteases, lipases, and lecithinases for each isolate. Isolates grouped into five coherent clusters, predominated by the species P. putida (cluster A), P. fluorescens (cluster B), P. fragi (as identified by Biolog) or P. fluorescens (as identified by API 20 NE) (cluster C), P. fragi (as identified by Biolog) or P. putida (as id

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David Kamei and Archana U Singh. "Studies on the isolation, identification and enzyme activity of bioagent Pseudomonas fluorescens used for controlling brown spot disease of rice caused by Helminthosporium oryzae." World Journal of Advanced Research and Reviews 12, no. 2 (2021): 458–61. http://dx.doi.org/10.30574/wjarr.2021.12.2.0564.

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In the present investigation studies was carried out ontheIsolation, Identification and Enzyme activity of bioagent Pseudomonas fluorescens used for controlling Brown spot disease of Rice caused by Helminthosporium oryzae(Breda de Haan).This is a fungal pathogen causing major disease that causes enormous losses in grain yield (upto 90%) particularly when leaf spotting phase assumes epiphytotic proportions.

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Kim, Jung-Soo, Manish Kumar Tiwari, Hee-Jung Moon, et al. "Identification and characterization of a novel nitrilase from Pseudomonas fluorescens Pf-5." Applied Microbiology and Biotechnology 83, no. 2 (2009): 273–83. http://dx.doi.org/10.1007/s00253-009-1862-6.

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Mulet, Magdalena, María José Martínez, Margarita Gomila, et al. "Genome-Based Species Diversity Assessment in the Pseudomonas chlororaphis Phylogenetic Subgroup and Proposal of Pseudomonas danubii sp. nov. Isolated from Freshwaters, Soil, and Rhizosphere." Diversity 15, no. 5 (2023): 617. http://dx.doi.org/10.3390/d15050617.

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The Pseudomonas chlororaphis phylogenetic subgroup of species, within the Pseudomonas fluorescens group, currently includes seven bacterial species, all of which have environmental relevance. Phylogenomic analyses help clarify the taxonomy of strains in the group and allow for precise identification. Thirteen antibiotic-resistant strains isolated in a previous study from nine different sampling sites in the Danube River were suspected to represent a novel species and are investigated taxonomically in the present study, together with four other strains isolated from the Woluwe River (Belgium) t

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Shilev, Stefan, Almudena Fernández López, María Sancho Prieto, and Enrique David Sancho Puebla. "INDUCED PROTEIN PROFILE CHANGES IN ARSENATE TOLERANT AND SENSITIVE PSEUDOMONAS FLUORESCENS STRAINS." JOURNAL OF ENVIRONMENTAL ENGINEERING AND LANDSCAPE MANAGEMENT 15, no. 4 (2007): 221–26. http://dx.doi.org/10.3846/16486897.2007.9636934.

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Pseudomonas fluorescens are gram‐negative, motile, rod‐shaped bacteria known for their metabolic versatility. Many strains of this species possess excellent capability to colonize plant roots, promoting plant growth in soils contaminated with toxic metals. P. fluorescens biotype F is a strain tolerant to arsenic which had been isolated previously from soil contaminated with arsenic and other toxic metals and characterized as a promoter of plant growth and accumulation of arsenic. In the present work we studied the protein profile of this strain together with the profile of a sensitive P. fluor

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Navitasari, Lisa, Loekas Soesanto, and Ahadiyat Yugi Rahayu. "PENGARUH APLIKASI PSEUDOMONAS FLUORESCENS P60 TERHADAP MUTU PATOLOGIS, MUTU FISIOLOGIS, DAN PERTUMBUHAN BIBIT PADI IR 64." Jurnal Hama dan Penyakit Tumbuhan Tropika 13, no. 2 (2013): 179–90. http://dx.doi.org/10.23960/j.hptt.213179-190.

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Effect of Pseudomonas fluorescens P60 on pathological and physiological quality and growth of rice IR 64 seedlings. The research objectives were (1) detection and identification of seed-borne pathogens of IR 64 rice, (2) testing Pseudomonas fluorescents P60 in inhibiting the in vitro growth of seed-borne pathogens colonies, (3) testing P. fluorescents P60 for pathological and physiological seed quality, and (4) testing P. fluorescents P60 on the growth of seedlings in the greenhouse. The results showed that some seed-borne pathogens can be found both on farmers’ IR 64 rice and factory’s; they

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Yao, Fulgence Koffi, Traoré Moumouny, Armist Tehua Amoa, Marthe Ehui Adjoua, and Aimée Mireille Alloue-Boraud Wazé. "Reduction of fungal spoilage in Amélie variety mango (Mangifera indica L.) using biocontrol agents." GSC Biological and Pharmaceutical Sciences 29, no. 3 (2024): 165–73. https://doi.org/10.5281/zenodo.14891016.

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The mango is today one of the five most produced and consumed fruits in the world after the orange, the banana, the grape and the apple. It is one of the three most exported fruits from Côte d’Ivoire with banana and pineapple. However, the mango sector faces enormous post-harvest losses due to diseases and handling activities. It is in this context that the objective of this work is to reduce post-harvest losses of the <em>Amélie</em> variety mango, using formulations based on biocontrol agents (<em>Pseudomonas fluorescens</em> and <em>Bacillus subtilis</

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Jaber, Tariq, Vikram Saini, Laura Morris, James D. Como, Nitin Bhanot, and Zaw Min. "799. A Pseudo-Outbreak of Pseudomonas fluorescens Infections." Open Forum Infectious Diseases 8, Supplement_1 (2021): S494—S495. http://dx.doi.org/10.1093/ofid/ofab466.995.

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Abstract Background Pseudomonas fluorescens is a water-borne pathogen that has been associated with outbreaks from transfusion of contaminated blood products or medical equipment. Our institution had a cluster of cultures that grew an uncommonly encountered microbe P. fluorescens within a period of one week. This prompted an internal investigation. We summarize the investigational process that led to the resolution of this pseudo-outbreak. Methods We conducted a retrospective chart review of surgical and non-surgical patients with cultures positive for P. fluorescens from July 2nd to July 8th

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Zechman, James M., and John N. Labows Jr. "Volatiles of Pseudomonas aeruginosa and related species by automated headspace concentration – gas chromatography." Canadian Journal of Microbiology 31, no. 3 (1985): 232–37. http://dx.doi.org/10.1139/m85-045.

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The volatile metabolites of three strains of Pseudomonas aeruginosa and one strain each of Pseudomonas cepacia, Pseudomonas maltophilia, Pseudomonas fluorescens, and Pseudomonas putida were analyzed using an automated headspace concentrator incorporating a gas chromatograph. The procedure does not require sample preparation and automates the entire analytical sequence to yield reproducible profiles of volatile constituents. Gas chromatographic profiles of the volatile metabolites of each species were obtained using a 20-min concentration period and two fused silica capillary columns of differe

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Raaijmakers, Jos M., Robert F. Bonsall, and David M. Weller. "Effect of Population Density of Pseudomonas fluorescens on Production of 2,4-Diacetylphloroglucinol in the Rhizosphere of Wheat." Phytopathology® 89, no. 6 (1999): 470–75. http://dx.doi.org/10.1094/phyto.1999.89.6.470.

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The role of antibiotics in biological control of soilborne pathogens, and more generally in microbial antagonism in natural disease-suppressive soils, often has been questioned because of the indirect nature of the supporting evidence. In this study, a protocol for high pressure liquid chromatography/mass spectrometry is described that allowed specific identification and quantitation of the antibiotic 2,4-diacetylphloroglucinol (Phl) produced by naturally occurring fluorescent Pseudomonas spp. on roots of wheat grown in a soil suppressive to take-all of wheat. These results provide, for the fi

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Thomas, Smitha, and Lizzy Mathew. "Biological Control for Solanum melongena L. in Sustainable Agriculture." Current Agriculture Research Journal 11, no. 3 (2024): 826–39. http://dx.doi.org/10.12944/carj.11.3.13.

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The environment and ecosystem were disrupted by the extensive use of fertilizers and pesticides which are harmful to humans and animals. Nature unfolds a biological response to overcome the different types of hazardous agrochemicals, in the form of microorganisms which have the efficiency to encourage plant growth without disturbing the environment. We conducted a biological approach to control phytopathogenic agents by plant growth-promoting rhizobacteria (PGPR), capable of restraining the devastation by phytopathogen. Pseudomonads can cling to soil particles, motile, prototrophic, and antibi

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Koffi, Yao Fulgence, Mathias N’cho, Amoa Armist Tehua, Ornella Akissi Loukou, and Wazé Aimée Mireille Alloue-Boraud. "Biocontrol of Spoilage in Avocado (Percea americana Mill) Using Pseudomonas fluorescens and Bacillus subtilis BT2." Microbiology Research Journal International 34, no. 9 (2024): 86–100. http://dx.doi.org/10.9734/mrji/2024/v34i91483.

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The avocado (Persea americana Mill.) is a dicotyledonous plant of the Lauraceae family. It is a fruit rich in nutrients, which has many benefits for human health. It is especially appreciated for its taste quality. However, post-harvest diseases of avocados due to fungal attacks are one of the main constraints on conservation and marketing in Côte d’Ivoire. The aim is to achieve biological control of avocado spoilage fungal strains during post-harvest conservation using Pseudomonas fluorescens and Bacillus subtilis BT2. Thus, 200 sampled lawyers were used for this study. Identification of the

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Mavrodi, D. V., O. V. Mavrodi, B. B. McSpadden-Gardener, B. B. Landa, D. M. Weller, and L. S. Thomashow. "Identification of Differences in Genome Content among phlD-Positive Pseudomonas fluorescens Strains by Using PCR-Based Subtractive Hybridization." Applied and Environmental Microbiology 68, no. 10 (2002): 5170–76. http://dx.doi.org/10.1128/aem.68.10.5170-5176.2002.

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ABSTRACT Certain 2,4-diacetylphloroglucinol-producing strains of Pseudomonas fluorescens colonize roots and suppress soilborne diseases more effectively than others from which they are otherwise phenotypically almost indistinguishable. We recovered DNA fragments present in the superior colonizer P. fluorescens Q8r1-96 but not in the less rhizosphere-competent strain Q2-87. Of the open reading frames in 32 independent Q8r1-96-specific clones, 1 was similar to colicin M from Escherichia coli, 3 resembled known regulatory proteins, and 28 had no significant match with sequences of known function.

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