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1
McCafferty, Michael Campbell. "Ovine cell mediated immunity to Chlamydia psittaci." Thesis, University of Edinburgh, 1992. http://hdl.handle.net/1842/20002.
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Enzootic Abortion of Ewes (EAE) is an economically important disease of ewes, caused by the gram negative, intracellular bacterium, Chlamydia psittaci. The disease results in lamb loss from abortion and the perinatal death of weak lambs. Vaccines have controlled EAE for more than thirty years, however in the last decade vaccine efficacy has been poor. The primary aim of this project was to examine the cell mediated immune responses to C.psittaci in sheep and to identify potentially immunoprotective antigens for future vaccine studies, by their ability to stimulate both T-cell proliferation and cytokine production. Preliminary studies determined the parameters of an antigen driven, ovine lymphocyte transformation assay for C.psittaci, employing whole chlamydial elementary bodies (EB) as antigen. It was shown that peripheral blood mononuclear cells (PMBC) from post abortion animals would proliferate in response to chlamydial EB, although lymphocytes from naive ewes also proliferated to a lesser degree. Further studies in mice showed this latter response could be caused by a cross reaction with harmless, enteric bacteria. The development of proliferative responses of the PMBC to both EB and mitogens was also measured during gestation. Infection at this time led to the development of lasting T-cell responses and a transient suppression of the response to the T-cell mitogen, Con A. In addition, both mitogen and antigen specific responses were disrupted in the immediate pre-parturition period. These responses returned to control levels soon after lambing. The T-cell proliferative response was further characterised by probing chlamydial EB which had been separated by SDS page electrophoresis and blotted onto nitrocellulose. Individual antigens were then added to cultures of PBMC and T-cell lines generates from post abortion animals. Four antigens were identified with approximate weights of 70, 50, 38 and 30kDa which stimulated proliferation. The ability of individual chlamydial proteins to stimulate cytokine production in these cultures was also tested. The four antigens above also stimulated the production of γ-IFN in the PBMC and T-cell lines from all sheep tested. Finally, the importance of γ-IFN in a chlamydial infection was investigted in an in vivo mouse model, where neutralising γ-IFN with a monoclonal antibody resulted in an increase in the severity of infection in both thymic and athymic mice, when compared with control animals. Increased numbers of viable chlamydiae were isolated from tissues and increased pathological changes and serum interferon levels were demonstrated. The results presented in this thesis provide evidence for the involvement of cell mediated responses in ovine immunity to C.psittaci. Both T-cell proliferation and γ-IFN production can be measured, although how the responses interact with B-cells and antibody has yet to be elucidated.
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Brown, Jeremy Keith. "Immune regulation of Chlamydia psittaci persistence in sheep." Thesis, University of Edinburgh, 1999. http://hdl.handle.net/1842/22786.
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Chlamydia psittaci is the causative agent of ovine enzootic abortion (OEA), the single most common cause of diagnosed infectious ovine abortion in the United Kingdom. Diagnosis and control of OEA is complicated by the ability of C. psittaci to establish a chronic low level or persistent infection in ewes infected out-with pregnancy. Persistent C. psittaci infection was reproduced in vitro by maintaining infected ovine fibroblastic ST.6 cells in the presence of recombinant ovine interferon gamma (ROvIFN-γ) at physiologically relevant concentrations for up to 63 days after infection. ROvIFN-γ restricted C. psittaci growth in a dose dependent manner, concentrations of 25-100 units of biological activity per millilitre (U/ml) induced and maintained persistence whereas concentrations of 250 U/ml or more eradicated C. psittaci from infected cultures. Attempts to identify and characterise the source of IFN-γ in immune sheep using T-cell lines raised against recombinant C. psittaci proteins were unsuccessful. The mechanisms of ROvIFN-γ mediated restriction of C. psittaci were investigated. Pretreatment of ST.6 cells with recombinant interferon alpha or double stranded RNA at concentrations matched with ROvIFN-γ for anti-viral activity did not restrict C. psittaci growth in ST.6 cells, indicating that the mechanism through which ROvIFN-γ restricts C. psittaci in ovine cells is independent from its anti-viral activities. Nitric oxide was not detected in the supernates of infected ST.6 cells pretrated with ROvIFN-γ and addition of L-NMMA, a potent inhibitor of inducible nitric oxide synthase, did not alter the anti- C. psittaci effects of ROvIFN-γ in this cell line. Furthermore, nitric oxide production could not be demonstrated in primary ovine alveolar macrophages treated with combinations of ROvIFN-γ, LPS, heat inactivated C. psittaci and/or live C. psittaci. Addition of exogenous L-tryptophan partially reversed the anti-chlamydial effects of ROvIFN-γ in ST.6 cells that had been infected with C. psittaci over a 48 hours period and also resulted in the recovery of infectious Chlamydiae from persistently infected ST.6 cells maintained in 50 U/ml of ROvIFN-γ. These findings support a role for up-regulation of tryptophan catabolism in ROvIFN-γ mediated restriction of both C. psittaci growth and persistence in ovine cells.
3
Hughes, Susan Elizabeth. "Protective humoral immune responses against ovine abortifacient Chlamydia psittaci." Thesis, University of Edinburgh, 1998. http://hdl.handle.net/1842/29811.
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Breakdown of a native chlamydial vaccine against ovine enzootic abortion (OEA), coupled with the identification of the protective capacity of the major outer membrane protein (MOMP) of C. psittaci, has encouraged research into the use of recombinant forms of MOMP in a third generation OEA vaccine. The prospect of testing all recombinant forms of MOMP in pregnant sheep trials is a daunting one, both in terms of time and money. To circumvent these problems, it was decided that a model system would be required to assess the efficacies of recombinant MOMP constructs. The initial model developed (active immunisation of mice) did not appear to reflect immunisation in sheep. Difficulties were encountered in eliciting seroconversion in mice vaccinated with recombinant antigens. It also demonstrated a possible H-2 link to the MOMP-specific antibody response. Problems associated with the model were overcome to some extent, by the development of a second model (passive transfer of sheep sera to mice). In this case, it was demonstrated that infection of mice susceptible to C. psittaci, could be reduced by passively transferring sera from sheep which had been vaccinated with recombinant antigens. The mechanism of neutralisation in vivo was also examined for this model. The model was further used to identify protective regions within the MOMP by passive transfer of affinity purified antibodies and monoclonal antibodies. Significant protection was afforded by the VS1 and VS2 regions of the MOMP.
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Hulin, Virginie. "Circulation des Chlamydiaceae en filières avicoles, exposition des professionnels et étude de la survie de Chlamydia psittaci." Thesis, Paris Est, 2016. http://www.theses.fr/2016PESC1016/document.
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La chlamydiose aviaire, causée par la bactérie Chlamydia psittaci, représente un risque zoonotique important. L’infection chez l’animal est principalement asymptomatique, mais chez l’Homme elle peut entrainer des pneumopathies atypiques sévères et causer la mort dans les cas les plus graves. Les personnes infectées sont principalement celles exposées régulièrement à des oiseaux, particulièrement dans le cadre professionnel. En France, de nombreux cas humains sont liés à une exposition à des canards Mulard, espèce utilisée pour la production du foie gras. Afin d’évaluer la prévalence des Chlamydiaceae chez les volailles et de caractériser les souches circulantes, des suivis ont été réalisés à différents stades de l’élevage (couvoir, pré-gavage et gavage pour la filière canard, et abattoirs impliqués dans l’abattage de différentes espèces aviaires). Des prélèvements d’air et de poussières ainsi que des suivis sérologiques et biologiques de personnels volontaires ont été effectués en parallèle afin d’évaluer l’exposition des professionnels travaillant au contact des volailles. Des prélèvements environnementaux ainsi que des essais in vitro visant à étudier la survie de C. psittaci ont été réalisés afin de tenter de mieux caractériser les voies de contamination des oiseaux, ce qui pourrait, à terme, permettre de maitriser le risque de contamination par C. psittaci chez l’animal et donc de réduire l’exposition des professionnels. Les résultats ont démontré une prévalence importance de C. psittaci chez le canard Mulard, au contraire des autres volailles qui hébergent très majoritairement C. gallinacea. L’exposition des professionnels aux Chlamydia est réelle, tout au long du processus d’élevage des volailles, mais plus particulièrement en élevages de canards Mulard et à l’abattoir, aussi il convient pour les professionnels de se protéger à chaque contact avec les animaux. L’hypothèse d’une contamination environnementale des animaux se fait de plus en plus claire, avec notamment la mise en évidence d’un lien existant entre les procédures de nettoyage et désinfection et l’excrétion des canards, la description de la survie de C. psittaci en dehors de tout hôte vivant, ou encore la mise en évidence d’une possible survie de C. psittaci au sein d’A. castellanii. La mise au point de moyens de lutte efficaces permettant de réduire voire de supprimer l’excrétion chez les volailles est également nécessaire, dans le but de diminuer l’exposition des professionnelsAvian chlamydiosis is a factor of economic loss to the poultry industry as well as a risk for zoonotic transmission to human. Chlamydia psittaci is the primary avian chlamydial pathogen with zoonotic potential. Although being mainly asymptomatic in birds, it can cause a disease called “psittacosis” in humans, with severe atypical pneumonia that leads to death in the most severe cases. Persons affected are mainly those whose occupations put them at risk of exposure, and a number of recent reports in France have confirmed that most of the human cases seemed to be linked to poultry, especially mule ducks. Currently there is evidence suggesting that avian chlamydiosis in poultry involves a new chlamydial agent, namely C. gallinacea. In order to evaluate the presence of Chlamydiaceae in poultry and the exposure of workers, we conducted four studies in the poultry industries, in duck hatchery, breeding farms and slauhgterhouse, as well as a studie in two poultry slaughterhouses including samples from voluntary workers. Results showed an important asymptomatic carriage of C. psittaci by mule ducks and a real, invisible and unpredictable exposure of workers. The species C. gallinacea was really prevalent in poultry othe than ducks and we still ignore its impact on human. Contamination of animals on farm seems to be mainly made via the environment. In vitro studies have been done to examine the survival of C. psittaci as a function of temperature in a non-nutritiv middle and showed that viable bacteria were still detectable after two months. Finally, the possible interactions between C. psittaci and an amoeba, Acanthamoeba castellanii, were studied and seem to show that the bacteria was able to enter the amoeba but we still ignore if it can survive or not
5
Rayes, H. M. "Serological, cultural and experimental studies of Chlamydia psittaci from sheep." Thesis, University of Liverpool, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.233854.
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Tönnies, Kirsten. "Darstellung der Haltungsbedingungen von Ziervögeln anhand der Praxis in 50 Zoofachgeschäften in den Jahren 1994 bis 1996 und Beurteilung der dort vorgefundenen Haltungsbedingungen unter Berücksichtigung bestehender rechtlicher und anderer Vorgaben." Giessen VVB Laufersweiler, 2009. http://d-nb.info/99599403X/04.
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Saad, M. Z. "In vitro and in vivo studies on Chlamydia psittaci (ovis) infection." Thesis, University of Liverpool, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.380157.
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Johnson, F. W. A. "Studies on Chlamydia psittaci associated ovine foetopathy in the United Kingdom." Thesis, University of Liverpool, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.372676.
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Schmit, Jean-Luc. "Modèle expérimental de pneumopathie murine à Chlamydia psittaci : application à l'évaluation des antibiotiques." Paris 11, 1991. http://www.theses.fr/1991PA114840.
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Castan, Bernard. "Etude des pneumopathies a chlamydia observees dans un centre hospitalier general de janvier 1989 a avril 1992." Toulouse 3, 1992. http://www.theses.fr/1992TOU31144.
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Bilato, Dania <1979>. "Chlamydia psittaci nel colombo di città: aspetti anatomo-patologici, sierologici e biomolecolari." Doctoral thesis, Alma Mater Studiorum - Università di Bologna, 2012. http://amsdottorato.unibo.it/4700/.
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Scopo del lavoro della presente tesi è stato quello di valutare la prevalenza di Chlamydiaceae, ed in particolare Chlamydia psittaci, in una popolazione aviare con caratteristiche sinantropiche quale il colombo di città (Columba livia var. domestica) dell’areale veneziano. Per evidenziare il patogeno sono state utilizzate metodiche sierologiche, d’isolamento e biomolecolari tradizionali. Contestualmente, mediante tecnologie molecolari innovative, quali microarray ed MLVA (Multilocus VNTR Assay) sono stati valutati i genotipi di C. psittaci e le eventuali altre specie di Chlamydia presenti in tale popolazione. Inoltre, si è proceduto ad una classificazione delle lesioni anatomo-patologiche ed ad una loro correlazione con la presenza del patogeno. I risultati dimostrano che la prevalenza di C. psittaci nella popolazione oggetto dello studio è del 10%. Durante tale studio è stata dimostrata la presenza di un ceppo di C. psittaci atipico, in quanto non classificabile con le attuali tecniche a disposizione. La genotipizzazione dei ceppi di C. psittaci conferma la presenza nel colombo di città del genotipo B, E ed E/B, che solitamente risultano essere coinvolti con minore frequenza in episodi di infezione umana. Inoltre sono stati dimostrati alcuni ceppi classificati come Chlamydia spp., in quanto le metodologie applicate e le conoscenze attuali non permettono ulteriori distinzioni, prospettando la possibilità di un nuovo ceppo. Infine, attraverso l’analisi dei dati raccolti durante la prima fase di campionamenti e successivamente confermati durante la seconda fase, siamo riusciti a strutturare un sistema di selezione, basato su caratteristiche funzionali ed anatomopatologiche, che permette di selezionare in sede necroscopica i colombi molto probabilmente infetti, permettendo conseguentemente una migliore organizzazione e gestione dei campioni di interesse contenendo nel contempo i costi ma mantenendo elevati gli standards diagnostici.The aim of this thesis was to evaluate the prevalence of Chlamydiaceae, in particular of C. psittaci, in sinanthropic birds such as urban pigeons (Columba livia var. domestica) in some areas of Venice. The pathogen was detected with serological, molecular, and traditional culture methods. Innovative molecular tools, such as microarray and MLVA (Multilocus VNTR Assay), were applied in this study in order to evaluate the genotypes of C. psittaci and the other species of Chlamydia present in this avian population to assess the risk of zoonosis posed by pigeons in this urban area. Moreover, we classified and correlated the anatomical and pathological lesions with the pathogen. Our results showed the presence of C. psittaci in urban population of pigeons in Venice, with a prevalence of 10%. We also demonstrated an atypical strain of C. psittaci not yet classified with the available laboratory techniques. Genotyping revealed the presence of genotypes B, E and E/B. This genotypes could be considered less frequently involved in cases of human infection. Additionally, we found other Chlamydia strains suggesting the presence of a new Chlamydia genotype. Finally, the elaboration of the data, collected during the first and second sampling phase, revealed a correlation between C. psittaci and adult females pigeons, presenting hepatomegaly. Based on this results we develop and adopted a diagnostic protocol during necropsy that allows to select pigeons, which have a higher probability to be infected, and a better organization and management of interests samples, containing the economic costs and maintaining high-level of the diagnostic standards.
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De, Sa Carlos. "Caractérisation d'antigènes de Chlamydia psittaci : intérêt pour la protection et le diagnostic." Tours, 1996. http://www.theses.fr/1996TOUR3803.
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Braz, Maria Amador. "Detecção e caracterização molecular de Chlamydophila psittaci e Chlamydophila abortus em aves assintomáticas /." Araçatuba : [s.n.], 2012. http://hdl.handle.net/11449/94591.
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Orientador: Marcelo Vasconcelos MeirelesBanca: Raphael Lúcio Andreatti Filho
Banca: Gisele Fabrino Machado
Resumo: Chlamydophila psittaci é uma bactéria que causa doença respiratória ou sistêmica em aves e em seres humanos. Há ainda, alguns relatos de infecção em aves por Chlamydophila abortus, que é um agente etiológico de problemas reprodutivos em mamíferos. Em vista do risco de transmissão para humanos a partir de aves assintomáticas o objetivo deste estudo foi detectar a presença de C. psittaci e C. abortus em amostras de fezes ou suabes cloacais de aves assintomáticas. Foram colhidas 403 amostras fecais ou suabes cloacais, provenientes de aves domésticas, selvagens ou exóticas, mantidas em cativeiro ou oriundas de apreensão. As amostras foram submetidas à PCR em tempo real para C. psittaci e C. abortus, para amplificação de fragmento parcial do gene da subunidade 16S do rRNA, utilizando o SsoFast™ EvaGreen® Supermix (Bio-Rad) e análise da curva de dissociação. Para determinação do genótipo de C. psittaci, foi utilizada a hemi- nested PCR específica para o gene OMP-A, realizada nas amostras positivas pela PCR em tempo real, seguida de sequenciamento dos fragmentos amplificados. A PCR em tempo real revelou positividade em 17 (4,21%) amostras. A hemi-nested foi positiva em 2 amostras positivas pela PCR em tempo real. O genótipo A de C. psittaci foi identificado pelo sequenciamento de uma amostra amplificada pela hemi-nested PCR
Abstract: Chlamydophila psittaci is a bacterium that causes respiratory or systemic disease in birds and humans. In birds there is also some reports of infection by Chlamydophila abortus that is responsible for abortions in mammals. Owing to the risk of transmission of Chlamydophila from asymptomatic birds to humans, the objective of this study was to detect the presence of C. psittaci and C. abortus in asymptomatic birds. Four hundred and three fecal samples or cloacal swabes were collected from domestic, wild or exotic birds kept in captivity or from apprehension. The 403 samples were examined by real time PCR specific for the 16S subunit of rRNA gene using SsoFastEvaGreen®Supermix™(Bio-Rad) and melting curve analysis. Hemi- nested PCR specific for the OMP-A gene, accomplished in real-time PCR positive samples, followed by sequencing of the amplified fragments were used to determine the genotype of C. psittaci. Real-time PCR was positive in 17 (4.21%) samples. Hemi-nested PCR revealed positivity in two samples previously positive by real-time PCR. Sequencing of the fragment amplified by hemi-nested PCR allowed for the identification of genotype A of C. psittaci in one sample
Mestre
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Braz, Maria Amador [UNESP]. "Detecção e caracterização molecular de Chlamydophila psittaci e Chlamydophila abortus em aves assintomáticas." Universidade Estadual Paulista (UNESP), 2012. http://hdl.handle.net/11449/94591.
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Chlamydophila psittaci é uma bactéria que causa doença respiratória ou sistêmica em aves e em seres humanos. Há ainda, alguns relatos de infecção em aves por Chlamydophila abortus, que é um agente etiológico de problemas reprodutivos em mamíferos. Em vista do risco de transmissão para humanos a partir de aves assintomáticas o objetivo deste estudo foi detectar a presença de C. psittaci e C. abortus em amostras de fezes ou suabes cloacais de aves assintomáticas. Foram colhidas 403 amostras fecais ou suabes cloacais, provenientes de aves domésticas, selvagens ou exóticas, mantidas em cativeiro ou oriundas de apreensão. As amostras foram submetidas à PCR em tempo real para C. psittaci e C. abortus, para amplificação de fragmento parcial do gene da subunidade 16S do rRNA, utilizando o SsoFast™ EvaGreen® Supermix (Bio-Rad) e análise da curva de dissociação. Para determinação do genótipo de C. psittaci, foi utilizada a hemi- nested PCR específica para o gene OMP-A, realizada nas amostras positivas pela PCR em tempo real, seguida de sequenciamento dos fragmentos amplificados. A PCR em tempo real revelou positividade em 17 (4,21%) amostras. A hemi-nested foi positiva em 2 amostras positivas pela PCR em tempo real. O genótipo A de C. psittaci foi identificado pelo sequenciamento de uma amostra amplificada pela hemi-nested PCR
Chlamydophila psittaci is a bacterium that causes respiratory or systemic disease in birds and humans. In birds there is also some reports of infection by Chlamydophila abortus that is responsible for abortions in mammals. Owing to the risk of transmission of Chlamydophila from asymptomatic birds to humans, the objective of this study was to detect the presence of C. psittaci and C. abortus in asymptomatic birds. Four hundred and three fecal samples or cloacal swabes were collected from domestic, wild or exotic birds kept in captivity or from apprehension. The 403 samples were examined by real time PCR specific for the 16S subunit of rRNA gene using SsoFastEvaGreen®Supermix™(Bio-Rad) and melting curve analysis. Hemi- nested PCR specific for the OMP-A gene, accomplished in real-time PCR positive samples, followed by sequencing of the amplified fragments were used to determine the genotype of C. psittaci. Real-time PCR was positive in 17 (4.21%) samples. Hemi-nested PCR revealed positivity in two samples previously positive by real-time PCR. Sequencing of the fragment amplified by hemi-nested PCR allowed for the identification of genotype A of C. psittaci in one sample
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Wyllie, Susan. "Structural and functional characterisation of the major outer membrane protein of Chlamydia psittaci." Thesis, University of Edinburgh, 1999. http://hdl.handle.net/1842/22762.
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The major outer membrane protein (MOMP) of Chlamydia shares several biochemical properties with classical porin proteins. To directly test the "porin channel" hypothesis at the molecular level, MOMP was reconstituted into planar lipid bilayers where it gave rise to "triple-barrelled" channels which were modified by an anti-MOMP neutralising monoclonal antibody. These observations are consistent with the well characterised homo-oligomeric nature of MOMP previously revealed by biochemical analysis, and the "triple-barrelled" behaviour of other porins. MOMP channels were weakly anion selective (PCl/PK ~ 2) and permeable to ATP. They may therefore be a route by which Chlamydia can take advantage of host nucleoside triphosphates, and explain why some anti-MOMP antibodies neutralise infection. In order to undertake more detailed studies of the MOMP structure/function relationship, recombinant MOMP from both C. psittaci and C. pneumoniae have been cloned and expressed. The recombinant proteins were functionally reconstituted in planar lipid and analysed at the single channel level. Both form porin-like ion channels that are functionally similar to the native protein. The C. psittaci recombinant porin was modified by the same anti-MOMP neutralising monoclonal antibody that effected the native protein. In contrast to the native protein, both recombinant C. psittaci (PCl/K ~ 0.38) and C. pneumonia (PCl/PK ~ 0.49) proteins were marginally cation selective. This is the first time native function has been demonstrated for recombinant chlamydial MOMP and will have an important impact on the future development of subunit vaccines.
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POUSSIN, MATHILDE. "Modalites de resistance de la souris balb/c vis a vis de chlamydia psittaci." Amiens, 1997. http://www.theses.fr/1997AMIE0106.
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Les chlamydiae sont des bacteries a multiplication intracellulaire obligatoire qui comprennent 4 especes : c. Trachomatis et c. Pneumoniae, qui infectent presque exclusivement l'etre humain, mais aussi c. Psittaci et c. Pecorum, qui touchent principalement les animaux. C. Psittaci est l'espece qui a la plus large gamme d'hotes. La souris balb/c fait partie des races de souris naturellement sensibles a la souche loth de c. Psittaci, qui est une souche d'origine aviaire. Les souris meurent 3 a 10 jours apres l'inoculation. L'injection prealable de la souche d'origine mammalienne a/22 suscite la protection des souris quand elles sont inoculees ensuite avec la souche loth. La protection ne peut etre conferee par le transfert de serum de souris immunisees mais elle peut l'etre par le transfert de cellules monoculeees, et en particulier de lt cd4#+, ce qui nous a conduit a nous interroger sur la nature de la sous-population cd4 la plus impliquee. Chez les souris immunisees en cours de guerison, le profil de production de cytokines des lt est essentiellement de type th1. Chez les souris non immunisees en train de mourir, les messagers produits sont preferentiellement ceux qui correspondent a une reponse de type th2. Cette dominante th1 de la protection est confirmee par la nature des anticorps produits par les souris en reponse a l'infection. Chez les souris immunisees, la quantite de cellules productrices d'igg#2#a specifiques augmente rapidement apres l'inoculation de la souche loth, alors qu'on ne trouve que peu de cellules productrices d'igg#1. Les souris non immunisees meurent trop rapidement pour qu'une telle etude ait pu etre menee. L'etape suivante consistait a identifier la nature et le mode d'action des cellules effectrices. Plusieurs types cellulaires pouvaient raisonnablement etre soupconnes dans ce modele, en particulier les lt cd8#+ et les macrophages actives. Nous avons suivi l'evolution des populations de lt cd8#+ et de macrophages, ainsi que celle des lt c d4#+, au niveau du peritoine des souris infectees par la souche loth. En effet, c'est a ce niveau que se fait l'inoculation de cette souche. Nous avons pu observer une difference dans les proportion de lt cd4#+ entre souris immunisees et non immunisees, ainsi qu'une augmentation, probablement due a un recrutement, du pourcentage de lt cd4#+ au cours du temps chez les souris protegees. En ce qui concerne les lt cd8#+ nous n'avons pas note de difference remarquable entre les differents cas de figure. Par contre, les macrophages se sont reveles plus interessants. En effet, si l'expression du cd11b (mac-1) changeait peu, mac-3, qui signale les macrophages peritoneaux, etait beaucoup plus eleve chez les souris immunisees. Pour verifier que ces macrophages etaient actives, nous avons etudie par rt-pcr l'expression des messagers de la no synthase inductible. Nous avons pu constater que, des le troisieme jour suivant l'inoculation de la souche loth, ces arn etaient exprimes chez les souris immunisees, alors qu'il etaient absents chez les souris non protegees. Nous avons par ailleurs pu verifier qu'une telle activation, quoique plus tardive, chez des souris ayant recu des cellules de souris immunisees, alors qu'on ne la retrouve pas chez les souris temoin ayant recu des cmn de souris non immunisees. Nous avons aussi pu verifier in vitro que les lt cd8#+ des souris immunisees possedent une fonction cytotoxique h-2 restreinte vis a vis de cellules histocompatibles infectees par la souche a/22. Il semble neanmoins que leur efficacite depende du rapport effecteur/cible. Il semble donc que les macrophages actives puissent effectivement impliques in vivo lors de la disparition des bacteries. L'action eventuelle des lt cd8#+ est plus sujette a caution. Des etudes complementaires devront neanmoins etre menees pour mesurer l'importance de l'intervention d'autres types cellulaires. Il faudra aussi determiner quels sont les constituants de la souche a/22 qui permettent l'induction d'une reponse protectrice, afin de pouvoir a terme mettre au point un vaccin.
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Abong, Bwemba Thérèse. "Mise au point d'un nouveau modèle expérimental de maladie murine à Chlamydia psittaci : son utilisation pour l'évaluation de l'activité de différentes molécules antibiotiques sur les Chlamydia." Paris 11, 1988. http://www.theses.fr/1988PA114811.
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Al-Amin, Dahiru Jibrilla. "Studies on ovine abortion with particular reference to the BS isolate of Chlamydia psittaci (ovis)." Thesis, Royal Veterinary College (University of London), 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.337453.
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Griffiths, Peter Charles. "Antigenic and molecular studies of Chlamydia psittaci and Chlamydia pecorum in ruminants : characterisation and diagnosis." Thesis, University of Southampton, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.313152.
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Froesch, Patrizia Rachele. "L'infezione da "Chlamydia psittaci" nei linfomi degli annessi oculari in una casistica di pazienti cubani /." [S.l.] : [s.n.], 2007. http://www.ub.unibe.ch/content/bibliotheken_sammlungen/sondersammlungen/dissen_bestellformular/index_ger.html.
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Frutos, María Celia. "Eco-epidemiología de Chlamydia psittaci, Chlamydia pneumoniae y chlamydia pecorum : Impacto en la Salud Pública." Doctoral thesis, Frutos MC. Eco-epidemiología de Chlamydia psittaci, Chlamydia pneumoniae y chlamydia pecorum : Impacto en la Salud Pública [Internet]. Universidad Nacional de Córdoba; 2015 [citado el 13 de febrero de 2020]. Disponible en: https://rdu.unc.edu.ar/handle/11086/6686, 2015. http://hdl.handle.net/11086/6686.
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Tesis - Doctorado en Ciencias de la Salud - Universidad nacional de Córdoba. Facultad de Ciencias Médicas. Secretaria de Graduados en Ciencias de la Salud, 2015168 h. : il
Zoonotic infections are a growing threat to global health. Atypical pneumonias are often caused by zoonotic pathogens such as Chlamydia. However; very little is known about chlamydial infections and their implications in our region The aim of this study was to enhance the eco-epidemiological knowledge of Chlamydia species in the province of Córdoba, Argentina. Serological and molecular techniques were implemented for the detection, quantification and genetic characterization of Chlamydia from a wide range of human samples [healthy individuals (n = 314) and individuals with suspected human psittacosis (n = 44) as well as animal samples [wild birds (n = 505), captive birds (n = 288), reptiles (n = 30), horses (n = 30)]. C. pneumoniae was the most frequently detected species in humans, followed by C. psittaci and C. pecorum. Co-infections were also detected. We did not find associated with sex, age, specific clinical conditions, seasonal pattern, or avian contact. However, atypical pneumonia was the main clinical manifestation associated with these agents. Mixed infections were associated with increased DNA quantification and an exacerbation of clinical symptoms, leading to hospitalization of patients who required intensive care.
Las infecciones zoonóticas son una creciente amenaza para la salud mundial. Las neumonías atípicas son causadas frecuentemente por patógenos zoonóticos como por ejemplo Chlamydia; sin embargo, varias de estas especies bacterianas y sus implicancias son aún poco conocidas. El objetivo del estudio fue profundizar en el conocimiento eco-epidemiológico de las especies de Chlamydia de importancia médico-veterinaria presentes en la provincia de Córdoba, Argentina. Para tal fin, se implementaron técnicas serológicas y moleculares para la detección, cuantificación y caracterización genética de Chlamydia en un amplio rango de muestras humanas [individuos sanos (n=314), individuos con nexo epidemiológico asociado a psitacosis (n=44) y animales [aves silvestres (n=505), aves en cautiverio (n=288), reptiles (n=30), equinos (n=30)]. La especie de Chlamydia más frecuentemente detectada en humanos fue C. pneumoniae, seguida de C. pecorum y C. psittaci. También se detectaron co-infecciones. Este hallazgo no pudo asociarse al sexo, edad, cuadros clínicos específicos, patrón estacional, ni especie aviar de contacto. Sin embargo, la neumonía atípica fue el cuadro clínico más fuertemente asociado al hallazgo de estos agentes y las infecciones mixtas estuvieron asociadas a mayor cuantificación bacteriana y a una exacerbación del cuadro clínico, llevando a la hospitalización de los pacientes, quienes requirieron cuidados intensivos.
Fil: Frutos, María Cecilia. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Instituto de Virología Dr. José María Vanella; Argentina.
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Ferreira, Vivian Lindmayer. "Avaliação sazonal do perfil sanitário de pombos-domésticos (Columba livia) em áreas de armazenamento de grãos e sementes no Estado de São Paulo." Universidade de São Paulo, 2012. http://www.teses.usp.br/teses/disponiveis/10/10133/tde-09102012-141850/.
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Columbiformes sinantrópicos podem ter um importante papel na epidemiologia de patógenos com potencial zoonótico ou de impacto econômico para a indústria avícola. Dentre eles destacam-se: Mycoplasma spp., Salmonella spp., paramixovírus aviário tipo 1 (APMV-1), inseridos no Programa Nacional de Sanidade Avícola (PNSA) e a Chlamydophila psittaci, agente de uma das principais zoonoses relacionada com aves silvestres. Dentro desse contexto, este trabalho objetivou pesquisar, sazonalmente, a ocorrência destes patógenos em pombos-domésticos (Columba livia) em dois entrepostos no Estado de São Paulo. Ao longo de um ano, mensalmente 10 pombos foram capturados em cada entreposto para a colheita de amostras de suabe cloacal e sangue. A técnica de soroaglutinação rápida em placa (SAR) foi utilizada para a detecção de anticorpos anti-M. synoviae, anti-M. gallisepticum e anti-S.Pullorum/Gallinarum; para a confirmação dos sororeagentes foram utilizadas a prova de inibição da hemaglutinação e soroaglutinação lenta, respectivamente. Para a detecção do DNA de C. psittaci e RNA de AMPV-1 foram utilizados métodos moleculares, PCR e RT-PCR. Para investigação de anticorpos anti-APMV-1 foi empregada a técnica de HI. Na SAR, 3,3% dos soros foram reagentes para M. synoviae; 2,5% para M. gallisepticum e 0,4% para S. Pullorum/Gallinarum. No entanto, essas amostras foram negativas nas técnicas confirmatórias. A ocorrência do APMV-1 não foi detectada. O DNA de C. psittaci foi detectado em 13,3% das amostras sendo 10,8% provenientes de aves capturadas na estação seca e 15,8% na estação chuvosa. Tais resultados são relevantes, pois demonstram que a C. psittaci ocorre em pombos presentes em áreas públicas frequentadas por um grande número de pessoas. Frente à escassez de pesquisas realizadas em Columbiformes no país, novos estudos são necessários para a determinação do real risco que pombos-domésticos podem representar quanto à transmissão de patógenos para aves comerciais e a influência da sazonalidade na disseminação desses microrganismos.Columbiformes may play an important role in the epidemiology of pathogens with zoonotic potential or economic impact in the poultry industry. Among these pathogens there are Mycoplasma spp., Salmonella spp., Avian Paramyxovirus type 1 (APMV-1), included in the National Poultry Health Program (PNSA) and Chlamydophila psittaci, etiologic agent of an important zoonosis associated with wild birds. Thus, the aim of this study was to investigate, seasonally, the occurrence of the pathogens listed above in feral pigeons (Columba livia) in two warehouses in São Paulo State. During one year, 10 birds were captured monthly in each locality and cloacal swabs and blood samples were collected from each pigeon. The rapid seroagglutination test was performed for the detection of antibodies against M. synoviae, M. gallisepticum and Salmonella Pullorum/Gallinarum. Positive results were submitted to the hemagglutination inhibition and slow seroagglutination test, respectively. For the C. psittacis DNA and APMV-1s RNA diagnosis, molecular techniques PCR and RT-PCR were performed. Hemagglutination inhibition test was also performed in order to detect antibodies against APMV-1. From the serum samples analyzed by rapid seroagglutination test, 3.3% were positive for M. synoviae, 2.5% for M. gallisepticum and 0.4% for S. Pullorum/Gallinarum. However, none of these samples was positive on the confirmatory tests. APMV-1 was not detected in any of the laboratory tests used. C. psittacis DNA was detected in 13.3% of the samples being, 10.8% from pigeons captured during the dry season and 15.8% in the rainy season. These results are relevant since they indicate that C. psittaci occurs in birds living in public areas frequented by a large number of people. The occurrence of the other pathogens was not detected. Nevertheless, due to lack of information about the pigeons sanitary status in the country, additional researches are necessary to determine the risk that feral pigeons can pose in the transmission of pathogens for poultry and the influence of each season in the spread of these microorganisms.
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Ostermann, Carola Heike [Verfasser]. "Evaluation and pathophysiological characterisation of a bovine model of respiratory Chlamydia psittaci infection / Carola Heike Ostermann." Berlin : Freie Universität Berlin, 2015. http://d-nb.info/1072072890/34.
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Möhle, Katja [Verfasser]. "Wirts-Erreger-Interaktionen im Respirationstrakt von Kälbern nach experimenteller aerogener Infektion mit Chlamydia psittaci / Katja Möhle." Hannover : Bibliothek der Tierärztlichen Hochschule Hannover, 2011. http://d-nb.info/1019022191/34.
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Silva, Stela Sampaio. "Avaliação clínica, laboratorial e detecção de Chlamydophila psittaci em calopsitas (Nymphicus hollandicus) do Distrito Federal, Brasil." reponame:Repositório Institucional da UnB, 2013. http://repositorio.unb.br/handle/10482/13618.
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Dissertação (mestrado)—Universidade de Brasília, Programa de Pós-Graduação em Saúde Animal, 2013.Submitted by Alaíde Gonçalves dos Santos (alaide@unb.br) on 2013-07-17T12:31:38Z No. of bitstreams: 1 2013_StelaSampaioSilva.pdf: 4584380 bytes, checksum: 258c41c6aa3b8715b3cac80fc95038bf (MD5)
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Psitacídeos, como as calopsitas (Nymphicus hollandicus), são frequentemente criados como animais de estimação. A ordem Psittaciforme é reconhecida como um dos principais reservatórios da bactéria Chlamydophila psittaci, causadora de uma zoonose. Essa bactéria por ser necessariamente intracelular, possui um diagnóstico difícil, normalmente sendo este por métodos sorológicos ou molecular, como a reação em cadeia da polimerase (PCR). A PCR é considerada o método de maior sensibilidade e especificidade para detecção da clamídia. Essa pesquisa visou analisar a ocorrência de C. psittaci em calopsitas de criadores e proprietários do Distrito Federal, através de técnicas de biologia molecular (PCR). Para tal, foram coletadas amostras de sangue e swabs oral e cloacal de 106 aves. Análises sanguíneas foram realizadas para tentar caracterizar eventuais efeitos da infecção sobre a saúde dos animais. Na amostra analisada a ocorrência foi de 0,9%, resultante de apenas uma calopsita positiva. Este animal apresentava diarreia, e escore corporal baixo, sinais clínicos que podem caracterizar infecção por C. psittaci. Não houve alterações hematológicas e bioquímicas na ave positiva, quando comparado com os valores das demais aves analisadas. Ao analisar os valores de hemograma, leucograma e bioquímica sérica das aves negativas para clamídia, quando avaliados fatores: peso, faixa etária, sexo e interação entre esses dois fatores, foi possível notar diferenças estatística em algumas variáveis. As variáveis enzimáticas (FA, AST, proteína total) e heterófilos absolutos são influenciadas pelo sexo, e o hematócrito, hemoglobina e eosinófilos pela faixa etária. A baixa ocorrência de clamídia encontrada pode ser explicada pela boa qualidade sanitária dos locais de origem dessas aves. _______________________________________________________________________________________ ABSTRACT
Psittacines such as cockatiel (Nymphicus hollandicus) are often raised as pet birds. The Order Psittaciforme is recognized as one of the main reservoirs of the Chlamydophila psittaci bacteria, the ethiological agent of a repiratory zoonosis. This bacteria is an obligate intracellular, usually diagnosed by serological or molecular methods, i.e. Polimerase Chain Reaction (PCR). The PCR is considered the test with the best sensitivity and specificity for chlamydia detection. The purpose of this dissertation is to analyse the C. psittaci occurence in cockatiels from commercial and non commercial breeders in Distrito Federal, through molecular technique (PCR). For this objective 106 birds were screened by oral and cloacal swab and blood sample. Blood analysis were performed to try to characterize haematological alterations effects on infected birds health. On this sample the prevalence found is 0.9%, from one positive tested cockatiel. The positive bird showed diarrhea, low body score, clinical signs that may characterize infection for clamydia. No haematological alterations were found on the positive bird in comparison to the other tested birds. The hemogram, leukogram, and serum biochemical on the chlamydia negative birds when compared with weight, age groups, sex and age-sex interation in some variables shown statistical difference. The enzimatic variables alkaline phostatase (ALP), aspartate transaminase (AST), total protein, absolute neutrophil count (ANC) are influenced by sex, haematocrit (HCT), haemoglubulin and eosinophils by age. The ocurrence of chlaymdia found may be explained by the good sanitary condition of the breeders facilities.
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Layachi, Khalid. "Typage de chlamydia psittaci isolées chez les ovins et amélioration du diagnostic de la chlamydiose abortive." Tours, 1990. http://www.theses.fr/1990TOUR3306.
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Prohl, Annette Charlotte [Verfasser]. "Evaluation of antimicrobial treatment strategies against Chlamydia psittaci using a bovine respiratory infection model / Annette Charlotte Prohl." Berlin : Freie Universität Berlin, 2016. http://d-nb.info/1088402100/34.
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Hirschhäuser, Thomas. "Indirekte und direkte Verfahren zum Nachweis von Chlamydophila psittaci-Infektionen bei deutschen Pferden unterschiedlicher Herkunft und Nutzung." Giessen VVB Laufersweiler, 2010. http://geb.uni-giessen.de/geb/volltexte/2010/7678/index.html.
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Ferreira, Lídia Lopes. "Detecção molecular da Chlamydophila psittaci em Columbiformes e Galliformes da região centro-sul do Estado de Goiás." Universidade Federal de Goiás, 2014. http://repositorio.bc.ufg.br/tede/handle/tede/4057.
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The chlamydiosis or ornithosis is a major zoonosis of avian origin and it is important for poultry breending. This study was carried out in order to investigate the presence of Chlamydophila psittaci in pigeons (Columba livia), turkeys (Meleagris gallopavo domesticus), broilers and free-range chickens (Gallus gallus domesticus) and to estimate the occurrence of infected animals in the Center-Souther region of the State of Goias. Cloacal and tracheal swabs were colleted fron 120 pigeons captured near farms, 300 broiler chickens, 240 turkeys and 240 free-range chickens, analyzed by polymerase chain reaction (PCR). We obtained by the PCR, 6,7% (8/120) positive trachea and cloaca swabs in pigeons, 5,8% (7/120) positive tracheas swabs, and 17,5 % (21/120) positive cloacal swabs, totaling 30% (36/120) of birds infected with C. psittaci. We did not detect the bacterium in samples from broilers and turkeys. We found 17,5% (42/240) positive free-range chickens, being 8,3% (20/240) positive trachea and cloaca swabs, 6,7% (16/240) positive swabs and 2,5% (6/240) cloacal swabs. We concluded that the birds as well free- range chickens are carries of C. psittaci while broilers and turkeys were not infected.
A clamidiose ou ornitose é uma das principais zoonoses de origem aviária e de importância para criações de aves domésticas. O presente trabalho foi realizado com o objetivo de pesquisar a presença de Chlamydophila psittaci em pombos (Columba livia), perus (Meleagris gallopavo domesticus), frangos de corte, galinhas caipiras (Gallus gallus domesticus) e estimar a ocorrência desses animais infectados na região Centro-Sul do Estado de Goiás. Foram capturados 120 pombos nas proximidades de agroindústrias, realizados 120 suabes traqueiais e 120 cloacais, também foram coletadas em 300 frangos de corte, 300 amostras de suabes cloacais e 300 de traqueiais. Em 240 perus foram realizados 240 suabes traqueiais e 240 cloacais e 240 suabes cloacais e 240 traqueais de galinhas caipiras de pequenas propriedades e feiras livres, os quais foram analisados por meio da reação em cadeia da polimerase (PCR). Obteveram-se pela PCR, 6,7% (8/120) pombos positivos para traqueias e cloacas, 5,8% (7/120) apenas para suabes de traqueia, 17,5% (21/120) apenas para suabes de cloacas, totalizando 30% (36/120) de aves infectadas com C. psittaci. Nas amostras oriundas de frangos de corte e de perus não se detectou a bactéria. Foram encontrados 17,5% (42/240) de galinhas caipiras positivas, sendo 8,3% (20/240) amostras positivas para traqueias e cloacas, 6,7% (16/240) apenas para suabes de traqueias e 2,5% (6/240) somente para suabes de cloacas. Conclui-se que os pombos assim como as galinhas caipiras deste estudo são portatores de C. psittaci, enquanto aos frangos e perus avaliados, não estavam infectados.
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Prohl, Annette [Verfasser]. "Evaluation of antimicrobial treatment strategies against Chlamydia psittaci using a bovine respiratory infection model / Annette Charlotte Prohl." Berlin : Freie Universität Berlin, 2016. http://d-nb.info/1088402100/34.
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Tomlinson, Lindsay. "Pathology of the ovine female reproductive tract, in particular the uterine tube, and its association with Chlamydia psittaci." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp03/NQ35814.pdf.
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Schüle, André. "Seroepidemiologische Studie zum Nachweis von Antikörpern gegen Coxiella burnetii und Chlamydophila psittaci bei Zootieren und den betreuenden Personen /." Berlin : Mbv, 2008. http://d-nb.info/991427335/04.
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Dahlberg, Jenny. "Development of a triplex real-time PCR method for detection of Chlamydia pneumoniae, Chlamydia psittaci and Mycoplasma pneumoniae." Thesis, Uppsala universitet, Institutionen för biologisk grundutbildning, 2016. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-279508.
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Lambertz, Jacqueline [Verfasser]. "Untersuchungen zur Pathologie und Pathogenese der experimentellen aerogenen Infektion von Kälbern mit Chlamydia psittaci (nicht aviärer Herkunft) / Jacqueline Lambertz." Hannover : Bibliothek der Tierärztlichen Hochschule Hannover, 2011. http://d-nb.info/1013433750/34.
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Bowland, Sandra Lee. "Effects of the estrous cycle or Chlamydia psittaci infection on leukocyte subpopulations in the uterus and blood of ewes." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1998. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp03/MQ31811.pdf.
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Hirschhäuser, Thomas [Verfasser]. "Indirekte und direkte Verfahren zum Nachweis von Chlamydophila psittaci-Infektionen bei deutschen Pferden unterschiedlicher Herkunft und Nutzung / Thomas Hirschhäuser." Gießen : Universitätsbibliothek, 2010. http://d-nb.info/1061047547/34.
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Kästner, Julia [Verfasser], Hans Peter [Akademischer Betreuer] Saluz, Llyod [Akademischer Betreuer] Vaughan, and Angela [Akademischer Betreuer] Berndt. "Identifizierung von immunreaktiven Proteinen in Chlamydia psittaci-infizierten Rindern / Julia Kästner. Gutachter: Hans Peter Saluz ; Llyod Vaughan ; Angela Berndt." Jena : Thüringer Universitäts- und Landesbibliothek Jena, 2015. http://d-nb.info/1076038778/34.
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Baxter, Stuart Ian Forgan. "Development of molecular techniques for the detection of C. psittaci infection in sheep and the typing of chlamydial isolates." Thesis, University of Edinburgh, 1991. http://hdl.handle.net/1842/29882.
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The aim of this project was to use nucleic acid based techniques for the detection and typing of C.psittaci strains. A representative genomic library of C.psittaci, S26/3, was constructed and used to isolate the 16S rRNA and major outer membrane protein (MOMP) genes. The 16S rRNA gene was subcloned into a transcription vector to allow the generation of single stranded RNA probes complementary to native C.psittaci ovine abortion (OA), 16S rRNA. This anti-sense probe was used in an RNase protection test (Hybrid Duplex RNA Analysis-HYDRA). HYDRA was shown to be a sensitive and highly specific detection test for C.psittaci, OA strain infection in biological samples including ovine faeces, ovine and human placenta, ovine ileal and tonsilar tissues and ovine vaginal swabs. The same anti-sense probe was also used to detect C.psittaci OA strains by in situ hybridisation. Chlamydia-specific oligonucleotide primers were designed and polymerase chain reaction (PCR) amplification of both the 16S rRNA and MOMP genes was developed, at a preliminary level, as a detection test for infection by C.psittaci. The application of both Southern blot analysis and restriction endonuclease (RE) profiling of PCR amplified fragments (PCR-RE profiling), using the MOMP and 16S rRNA genes, allowed the identification of C.psittaci subspecies types and the easy discrimination of the three chlamydial species. A combination of Southern blot analysis, PCR-RE profiling and direct DNA sequence determination of the MOMP genes from a large group of C.psittaci,OA isolates was used to investigate possible strain variation within this C.psittaci type. The comparison demonstrated a high degree of identity within the OA group and a contrasting high level of variation within the other group of ovine C.psittaci, non-abortion ruminant strains. This study has established simple and convenient techniques for both detection and identification of the 2 different types of C.psittaci which infect sheep, independent of culture and extensive purification of the causative organism.
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Tan, Tin Wee. "Biochemical, immunological and genetic characterisation of the major outer membrane protein from an ovine abortion strain of Chlamydia psittaci." Thesis, University of Edinburgh, 1989. http://hdl.handle.net/1842/19339.
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Ovine enzootic abortion (OEA) is an economically important disease of ewes caused by a type of Chlamydia psittaci. Vaccines have been available to control this disease for more than 30 years. In the past decade, vaccine efficacy has been poor despite efforts to improve the inactivated whole organism vaccine currently in use. The initial aim of this project was to characterise the antigenic structure of OEA C.psittaci and to identify potentially immunoprotective antigens. Retrospective analyses of sera taken from ewes of different immune status were carried out using immunoblotting. A single 39 to 40 kDa antigen, thought to be the major outer membrane protein (MOMP) of OEA C.psittaci, was implicated as an important immunogen. A series of biochemical analyses including gel electrophoresis, immunoblotting, peptide mapping, surface radio-iodination, detergent fractionation, in vitro oligomerisation and protein microsequencing, as well as electron microscopy, showed conclusively that this antigen was indeed the MOMP of OEA C.psittaci, the analogue of well-characterised MOMPs of other chlamydial strains. OEA MOMP was found to be a major surface-exposed component of the outer membrane fraction of the chlamydial elementary body (EB), and appeared as fine ultrastructural particles (3 to 4 nm in diameter) densely packed on the outermost surface of chlamydial EBs. It possessed epitopes cross-reactive with other chlamydial MOMPs and is a site of heterogeneity between ovine C.psittaci types. Its solubility was enhanced in the presence of reducing agent and MOMP monomers formed disulphide cross-linked oligomers under non-reducing conditions in vitro. It was found to possess an N-terminus identical to C.trachomatis MOMP indicating that cleavage of the signal sequence occurs at the same site to produce a mature protein of 39.5kDa. A method was adapted to isolate MOMP by detergent extraction. An outer membrane fraction, highly enriched in MOMP, was prepared in sufficient quantities for a vaccination-challenge experiment. The results showed that both purified whole elementary bodies and the outer membrane preparation, given in a single dose, could protect ewes from infection and abortion. To test the hypothesis that MOMP was a protective component in these vaccine preparations, sufficient quantities of purified MOMP were needed. A recombinant DNA approach was taken. Firstly, the MOMP gene from a vaccine strain, S26/3, was completely sequenced and extensively analysed in order to develop good strategies of expressing recombinant MOMP (rMOMP). This monocistronic gene contained putative tandem promoter and rho-independent terminator sequences flanking a 1,167 base-pair open reading frame. Comparison with other MOMP gene sequences revealed four highly variable domains interdigitating five constant domains. Using the polymerase chain reaction and specially designed primers, a specific sequence corresponding to the mature MOMP was amplified, cloned into M13 mptac18 viral expression vector and subcloned into three plasmid vectors, pUC8, pRIT5 and pRIT2T, for expression. Several rMOMPs representing the complete mature MOMP and a truncated MOMP were successfully expressed in Escherichia coli. Finally, further analyses showed that these rMOMPs retained antigenic properties of MOMP and that large quantities could be obtained in a highly purified form. Such rMOMPs can now be tested for the presence of epitopes which can protect pregnant ewes from ovine enzootic abortion.
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Nüchter, Heike. "Nachweis von Chlamydophila psittaci in unterschiedlichen Bereichen in zwei Hähnchen- und zwei Putenschlachtereien mittels direkter Immunfluoreszenz nach Erregeranzüchtung in Buffalo-Green-Monkey-Kidney-Zellkulturen sowie der Polymerase-Ketten-Reaktion mit anschliessender Restriktionsenzymanalyse." Wettenberg : VVB Laufersweiler, 2004. http://deposit.ddb.de/cgi-bin/dokserv?idn=970941587.
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Schüle, André [Verfasser]. "Seroepidemiologische Studie zum Nachweis von Antikörpern gegen Coxiella burnetii und Chlamydophila psittaci bei Zootieren und den betreuenden Personen / André Schüle." Berlin : Freie Universität Berlin, 2008. http://d-nb.info/1027497357/34.
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Styles, Darrel Keith. "Psittacid herpesvirus associated with internal papillomatous disease and other tumors in psittacine birds." Texas A&M University, 2005. http://hdl.handle.net/1969.1/2646.
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Internal papillomatous disease (IPD) is characterized by mucosal papillomas
occurring primarily in the oral cavity and cloaca of Neotropical parrots. These lesions
can cause considerable morbidity, and in some cases result in mortality. Efforts to
demonstrate papillomavirus DNA or proteins in the lesions have been largely
unsuccessful. However, increasing evidence suggests that mucosal papillomas may
contain psittacid herpesviruses (PsHVs). In this study, PsHV 1 genotype 1, 2, and 3
DNA was found in 100% of mucosal papillomas from 30 Neotropical parrots by PCR
using PsHV specific primers. However, Psittacus erithacus papillomavirus and finch
papillomavirus DNA were not detected. Additionally, a novel PsHV sequence related
to, but phylogenetically distinct from PsHV 1, was identified in 4 African grey parrots
(Psittacus erithacus), two of which exhibited papillomas. These findings suggest that
mucosal papillomas may develop in parrots latently infected with PsHV. Tumors of the
bile and pancreatic ducts have also been observed in parrots with IPD. Other mucosal
tumors including carcinomas of the proventriculus and ventriculus may be coincident with bile duct tumors, but cloacal carcinomas usually develop as solitary lesions. To test
whether PsHV was associated with these tumors, the fresh tissues from 11 parrots and
the formalin-fixed paraffin-embedded (FFPE) tissues of 5 parrots exhibiting mucosal
tumors were examined by PCR. All tumors were found to contain PsHV 1 genotype 3
DNA except one bird with a cloacal carcinoma that contained genotype 4.
Histologically normal tissues available from six parrots did not contain PsHV DNA.
Experiments were performed using the FFPE tissues of 5 parrots with IPD related
tumors known to contain PsHV by PCR, to show that the virus was in significantly
higher concentration in the neoplastic tissue compared to adjacent histologically normal
tissue. Neoplastic and adjacent unaffected cells were dissected from the tissues using
laser capture microdissection and the DNA was examined by PCR. In situ hybridization
using PsHV specific probes and direct in situ PCR were also performed on the tissues.
A strong association was shown between infection by PsHV 1 genotype 3 and birds
manifesting IPD related tumors and other neoplasms of the digestive tract.
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Shirey, Kari Ann. "Modulation of Interferon-gamma Receptor Expression During Infection with Chlamydia psittaci 6BC and Its Influence on Indoleamine 2,3-dioxygenase." Connect to this document online, 2006. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=miami1140715510.
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Thesis (Ph. D.)--Miami University, Dept. of Microbiology, 2006.Title from second page of PDF document. Document formatted into pages; contains [3], vi, 176 p. : ill. Includes bibliographical references (p. 131-176).
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Wolf, Katharina [Verfasser], Eberhard [Akademischer Betreuer] Straube, Hans Peter [Akademischer Betreuer] Saluz, and Andreas [Akademischer Betreuer] Essig. "Wirksamkeit von Antibiotika gegenüber replikativen und persistenten Formen von Chlamydia psittaci / Katharina Wolf. Gutachter: Eberhard Straube ; Hans Peter Saluz ; Andreas Essig." Jena : Thüringer Universitäts- und Landesbibliothek Jena, 2015. http://d-nb.info/1079840761/34.
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Thierry, Simon. "Etude de la diversité génétique d'Aspergillus fumigatus et de Chlamydophila psittaci chez les oiseaux et mise au point de modèles expérimentaux aviaires." Phd thesis, AgroParisTech, 2011. http://pastel.archives-ouvertes.fr/pastel-00603770.
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Le champignon filamenteux Aspergillus fumigatus et la bactérie Chlamydophila psittaci sont deux agents pathogènes majeurs chez les oiseaux d'élevage. Bien que phylogénétiquement très distants, ces deux micro-organismes partagent un même mode de transmission par voie aérienne. La mortalité et la morbidité associées aux aspergilloses ou aux chlamydioses ont un impact économique qui n'est pas négligeable, en particulier dans les élevages de dindes. En France, les investigations autour de cas humains de chlamydiose identifient fréquemment un lien avec des élevages de canards, chez lesquels l'infection par C. psittaci s'apparente à un portage intestinal asymptomatique. Pour analyser la circulation des agents pathogènes dans les élevages avicoles, nous avons tout d'abord étudié la diversité génétique d' Aspergillus fumigatus et de Chlamydophila psittaci. Pour cela, deux nouvelles méthodes de typage moléculaire MLVA (Multiple Locus VNTR Analysis) ont été mises au point. Ces méthodes se sont révélées très discriminantes, rapides et faciles à mettre en œuvre. Dans le cas d' A. fumigatus, la méthode MLVA a permis de regrouper les génotypes en fonction de leur origine géographique. Elle a également permis d'analyser les modes de contamination d'un couvoir de dindes. Dans un second temps, nous avons analysé le pouvoir pathogène de ces deux microorganismes chez les oiseaux. Pour cela, deux modèles d'infections expérimentales ont été développés. Le premier modèle a permis de reproduire une aspergillose chez des poussins de 1 jour après inhalation d'un aérosol contenant des conidies d'A. fumigatus. Lors de la mise au point du modèle, l'impact de l'immunodépression et de la lignée de poulet a été évalué. Pour C. psittaci, un modèle reproduisant un portage intestinal de la bactérie chez des canetons mulards de 1 jour a été obtenu.
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Vilela, Daniel Ambrozio da Rocha. "Diagnóstico de situação dos animais silvestres recebidos nos CETAS brasileiros e Chlamydophila psittaci em papagaios (Amazona aestiva) no CETAS de Belo Horizonte, MG." Universidade Federal de Minas Gerais, 2012. http://hdl.handle.net/1843/SMOC-9F4GS6.
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Efforts on preventing wildlife trade in Brazil have generated large numbers of rescued animals that are sent to wildlife rehabilitation centers (CETAS). However, stress, superpopulation and lack of biosecurity have been causing a diversity of problems. The objectives of this study were, to elaborate a diagnosis of rescued wildlife, with emphasis on identification of avian species, sent to all Brazilian CETAS, and to determine the occurrence of avian chlamydiosis in CETAS of Belo Horizonte, MG. Were counted 234,595 specimens, being mos birds, coming primarily from confiscations in the Southeast and Northeast regions. Orders received included as the most frequent were Passeriformes, Psittaciformes, and Columbiformes and families Emberezidae, Thraupidae, Psittacidae, Icteridae and Cardinalidae. The saffron finch (Sicalis flaveola), the green-winged Saltator (Saltator similis), the yellow-bellied seedeater (Sporophila nigricolis), the double-collared seedeater (Sporophila caerulescens) and ultramarine grosbeak (Cyanoloxia brissonii) were the predominant species. Approximately 25% of animals received in CETAS were rescued in urban environment or voluntarily delivery to institutions. Rescued animals were principally release into the wild (52%), died (24%) or destined to captivity (7%). For assessment of avian chlamidiosis, 212 psittacines were necropsied, evaluated for gross and histopathological findings and tested for C. psittaci DNA in livers (PCR). Of the total, 152 (71%) were positive for C. psittaci by PCR, most in a state of cachexia. Poor body condition, air sacculitis and hepato and splenomegaly with necrotic foci were the most common post mortem findings. The major microscopic lesions were inflammatory infiltrates and foci of necrosis in the liver and increased plasma cell differentiation in the spleen. Intracytoplasmic inclusion bodies were identified in 52% of hepatic and splenic tissues examined. The genetic sequence showed that only the genotype A was circulating among the population studied. The wildlife traffic impact on the conservation of biodiversity, the implications of reintroduction and the importance of diseases, especially avian chlamydiosis, in wildlife and human health are discussed.As ações de combate e fiscalização do tráfico de vida silvestre geram um quantitativo de animais que, via de regra, são encaminhados para os centros de triagem de animais silvestres (CETAS). Os objetivos do presente estudo foram elaborar um diagnóstico da fauna silvestre, com ênfase na avifauna, encaminhada aos CETAS do Brasil e verificar a ocorrência de clamidiose aviária no CETAS de Belo Horizonte, MG, no período de janeiro de 2008 a dezembro de 2010. Foram contabilizados 234.595 espécimes, a maioria dos animais pertencentes ao grupo das aves e procedentes principalmente de apreensões ocorridas nas regiões Sudeste e Nordeste. As ordens mais recebidas foram os Passeriformes, Psittaciformes e Columbiformes e as famílias Emberezidae, Thraupidae, Psittacidae, Icteridae e Cardinalidae foram as mais frequentes. O canário-da-terra (Sicalis flaveola), o trinca-ferro (Saltator similis), o coleiro-baiano (Sporophila nigricolis), o coleirinho (Sporophila caerulescens) e o azulão (Cyanoloxia brissonii) foram as espécies predominantes. Aproximadamente 25% dos animais encaminhados para os CETAS foram recolhidos nas cidades ou entregues voluntariamente pelas pessoas às instituições. A principal destinação proporcionada aos animais foi a realização de solturas (52%), seguida pelos óbitos (24%) e destinação para criadouros (7%). Para verificar a ocorrência de clamidiose aviária, foram avaliados 212 óbitos de Amazona aestiva por meio de exames necroscópicos, histopatológicos e moleculares. Deste total, 152 foram positivos para C. psittaci pela PCR, a maioria em estado de caquexia. As alterações macroscópicas mais frequentes foram aerossaculite, hepatomegalia com focos de necrose e esplenomegalia. As principais lesões microscópicas foram os infiltrados inflamatórios e focos de necrose no fígado e o aumento na diferenciação plasmocitária no baço. Foram identificados corpúsculos de inclusão intracitoplasmática em 52% dos tecidos hepáticos e esplênicos examinados. Por meio do sequenciamento genético verificou-se que o genótipo A foi o responsável pela infecção dos papagaios. Discute-se os impactos do tráfico na conservação da biodiversidade, as implicações das reintroduções e a importância das doenças, principalmente a clamidiose aviária, na fauna silvestre e saúde humana.
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González, Hein Gisela Andrea. "Estudio serológico de Chlamydophila psittaci, Salmonella spp., virus Pox aviar, adenovirus y virus polioma en aves del orden Psittaciforme en cautiverio en Chile central." Tesis, Universidad de Chile, 2006. http://repositorio.uchile.cl/handle/2250/130847.
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Memoria para optar al Título
Profesional de Médico VeterinarioMediante un estudio serológico se evaluó la presencia de infecciones virales por virus de la diftero viruela aviar (vDVA), adenovirus aviar (AVA) y virus polioma aviar (VPA) e infecciones bacterianas por Salmonella enteritidis, S. typhimurium y Chlamydophila psittaci en muestras de sangre provenientes de diez poblaciones de aves Psittaciformes en cautiverio: nativas (Enicognathus ferrugineus, Enicognathus leptorhynchus, Cyanoliseus patagonus bloxami) y exóticas (30 especies), localizadas en diversos lugares de la Región Metropolitana de Chile. Se demostró que las aves psitácidas incluidas en esta investigación presentaban seropositividad frente a infección por vDVA en 8 de 201 aves (4%); a AVA del grupo I en 9 de 184 aves (4,9%) y particularmente a VPA en 36 de 100 aves (36%). Estas infecciones virales, reconocidas por primera vez en aves psitácidas exóticas en Chile, también han sido comunicadas en este mismo tipo de aves en otros lugares del mundo. Pero se debe agregar que es la primera evidencia de infección por VPA, vDVA y AVA en psitácidas nativas en nuestro país. En este estudio, también se detectó la presencia de anticuerpos séricos contra C. psittaci en 11 de 49 aves (22,4%) y contra S. enteritidis en 2 de 184 aves (1,1%). No se detectó la presencia de anticuerpos séricos contra S. typhimurium en las 184 aves psitácidas monitoreadas
FAVET: Proyecto interno No.3647; Infectious Diseases Laboratory, College of Veterinary Medicine, University of Georgia, Athens, U.S.A.
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Yupanqui, Castañeda Carmen Milagros. "Detección de Chlamydia psittaci en guacamayos (Ara spp) y loros (Amazonas spp y Pionus sp) en cautiverio de un parque zoológico de Lima, Perú." Master's thesis, Universidad Nacional Mayor de San Marcos, 2015. https://hdl.handle.net/20.500.12672/5057.
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Publicación a texto completo no autorizada por el autorEvalúa la presencia de Chlamydia psittaci en 134 aves mantenidas en cautiverio de un parque zoológico de Lima, Perú. Realizó un aislamiento celular, mediante la inoculación de las muestras orofaríngeas en cultivos de células BGM, los cuales se llevaron a incubación 37°C por 6 días para continuar con la técnica de fijación, y bloqueo y posteriormente con la prueba de inmunofluorescencia directa para encontrar dicha bacteria. Las muestras fueron transportadas a Bélgica para su procesamiento, para lo cual fue necesario obtener los permisos/certificados de exportación e importación de cada país por ser procedentes de especies pertenecientes a los apéndices I y II anexos A y B de la lista del CITES (Convención sobre el comercio internacional de especies amenazadas de fauna y flora silvestre). Entre las aves muestreadas se tuvieron 6 especies de guacamayos: Ara ararauna (n=15), Ara chloroptera (n=18), Ara (Primolius) couloni (n=13), Ara macao (n=12), Ara militaris (n=8), Ara severa (n=2); 5 especies de loros de gran tamaño: Amazona amazonica (n=7), Amazona farinosa (n=14), Amazona festiva (n=5), Amazona mercenaria (n=5), Amazona ochrocephala (n=12) y 1 especie de loro de menor tamaño: Pionus menstruss (n=23). Al final del estudio, del total de aves estudiadas, se hallaron 24 (17.91%) aves positivas a Chlamydia psittaci; todas las especies del presente estudio dieron positivo, a excepción de Ara (Primolius) couloni. Esta es la primera vez que se diagnostica C. psittaci en Perú, específicamente, en aves psitácidas.
Tesis
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Friedrich, Lydia [Verfasser], Hans Peter [Akademischer Betreuer] Saluz, Dietmar [Akademischer Betreuer] Gottschild, and Jörg [Akademischer Betreuer] Kotzerke. "Etablierung des Hühnerembryonenmodells zur Darstellung von Infektionen mittels Positronenemissionstomographie / Computertomographie (PET / CT) am Beispiel von Chlamydophila psittaci [[Elektronische Ressource]] / Lydia Friedrich. Gutachter: Hans Peter Saluz ; Dietmar Gottschild ; Jörg Kotzerke." Jena : Thüringer Universitäts- und Landesbibliothek Jena, 2013. http://d-nb.info/1036872238/34.
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Böcker, Selina [Verfasser], Hans Peter [Akademischer Betreuer] Saluz, Thomas [Akademischer Betreuer] Munder, and Michael R. [Akademischer Betreuer] Knittler. "Funktionelle Charakterisierung der Interaktion zwischen dem Typ-III-sekretierten Protein IncB aus Chlamydia psittaci und dem humanen Protein Snapin / Selina Böcker. Gutachter: Hans Peter Saluz ; Thomas Munder ; Michael R. Knittler." Jena : Thüringer Universitäts- und Landesbibliothek Jena, 2015. http://d-nb.info/1066238308/34.
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