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Dissertations / Theses on the topic 'Psychrotrophic bacteria'

Author: Grafiati

Published: 4 June 2021

Last updated: 25 July 2025

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1

McCarthy, Conor Neil, and n/a. "Regulatory Elements Controlling Lipase and Metalloprotease Production in Pseudomonas fluorescens B52." Griffith University. School of Health Science, 2003. http://www4.gu.edu.au:8080/adt-root/public/adt-QGU20031015.124744.

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Psychrotrophic bacteria, such as Pseudomonas fluorescens B52, are a major cause of milk spoilage at refrigeration temperature due to the production of lipolytic and proteolytic enzymes. Regulatory mechanisms controlling the production of lipase and protease by the B52 lipA and aprX genes were investigated. Transposon mutagenesis identified the possible involvement of a poly-A polymerase enzyme which destabilises mRNA by 3' polyadenylation. A homologue of the E. coli EnvZ/OmpR two-component sensor/regulator system was identified by transposon mutagenesis and shown to repress lipase and protease

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2

McCarthy, Conor Neil. "Regulatory Elements Controlling Lipase and Metalloprotein Production in Pseudomonas fluorescens B52." Thesis, Griffith University, 2003. http://hdl.handle.net/10072/367432.

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Psychrotrophic bacteria, such as Pseudomonas fluorescens B52, are a major cause of milk spoilage at refrigeration temperature due to the production of lipolytic and proteolytic enzymes. Regulatory mechanisms controlling the production of lipase and protease by the B52 lipA and aprX genes were investigated. Transposon mutagenesis identified the possible involvement of a poly-A polymerase enzyme which destabilises mRNA by 3' polyadenylation. A homologue of the E. coli EnvZ/OmpR two-component sensor/regulator system was identified by transposon mutagenesis and shown to repress lipase and protease

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3

Suzuki, Takeshi. "Enzymological Studies of Cold-active Esterases from Psychrotrophic Bacteria." Kyoto University, 2003. http://hdl.handle.net/2433/149012.

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4

Gauthier, Elisabeth. "Utilization of low molecular weight substrates by psychrotrophic meat spoilage organisms." Thesis, McGill University, 1990. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=59274.

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Four meat spoilage organisms were grown at 4$ sp circ$C for 7 d, in an aqueous extract of meat (Meat Juice Medium), and the levels of various nutrients in the extracts were measured. At an agitation rate of 50 rev$ cdot$min$ sp{-1},$ the four species reached viable counts of 10$ sp8$ Colony Forming Units (CFU)$ cdot$ml$ sp{-1},$ and the order of nutrient utilization was as follows: (1) glucose, (2) gluconate and urea, (3) glycerol, (4) glucose-6-phosphate. Several substrates were still present in the growth medium at the end of the growth period, namely lactate, glucose-6-phosphate and the two

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5

Rasmussen, David Dean. "The Effectiveness of Potassium Lactate and Lactic Acid Against Campylobacter Species and Psychrotrophic Bacteria." Thesis, Virginia Tech, 1999. http://hdl.handle.net/10919/35318.

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This study examined the efficacy of potassium lactate and lactic acid to control Campylobacter sp. and psychrotrophic bacteria on chicken. The objectives of the two studies conducted were to determine the optimal combination of potassium lactate and lactic acid to inhibit Campylobacter sp. in a challenge study and to inhibit naturally occurring Campylobacter sp. and psychrotrophic bacteria in a shelf life study. <p> Boneless, skinless chicken breasts were injected with three levels of potassium lactate (0,1.5,2%), in conjunction with four levels of lactic acid. Lactic acid was injected

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6

Fitzgerald, Laura Emma. "The application of microfiltration as a partial sterilisation technique for the reduction of psychrotrophic spore forming bacteria from viscous dairy feeds." Thesis, University of Bath, 2012. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.582874.

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The use of microfiltration as an alternative to pasteurisation to reduce the microbial load of raw skimmed milk is a well established technology. However, its application in reducing bacteria from highly viscous dairy based solutions has not due to issues of low flux and high fouling tendency. This study involves the application of microfiltration to remove spores from high solids content Milk Protein Isolate (MPI) solutions. MPI feeds were inoculated with Bacillus mycoides spores a safer alternative to Bacillus cereus, a psychrotrophic spore forming bacteria found in dairy feeds. Suitable pro

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7

Voges, Joana Gerent. "Qualidade microbiológica da água e do leite e ocorrência de Leite Instável Não Ácido (LINA) em propriedades de agricultura familiar do Planalto Norte de Santa Catarina." Universidade do Estado de Santa Catarina, 2015. http://tede.udesc.br/handle/handle/954.

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Made available in DSpace on 2016-12-08T16:24:22Z (GMT). No. of bitstreams: 1 PGCA15MA173.pdf: 751760 bytes, checksum: 23f614e28b7b6a81f91e72de196f5cb7 (MD5) Previous issue date: 2015-06-29<br>Coordenação de Aperfeiçoamento de Pessoal de Nível Superior<br>Milk production is an important income for family farmers, but to act competitively in the market the dairy sector needs to raise the quality of its raw material. The study aimed to evaluate the factors that influence water quality and milk quality and unstable non-acid milk (UNAM) occurrence in family farms of Planalto Norte of Santa Catari

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8

Cardoso, Rodrigo Rezende. "Influência da microbiota psicrotrófica no rendimento de queijo Minas Frescal elaborado com leite estocado sob refrigeração." Universidade Federal de Viçosa, 2006. http://locus.ufv.br/handle/123456789/5390.

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Made available in DSpace on 2015-03-26T13:52:03Z (GMT). No. of bitstreams: 1 texto completo.pdf: 780684 bytes, checksum: 95e7e3b5d38de6b4ae7eeb032a065277 (MD5) Previous issue date: 2006-03-16<br>Coordenação de Aperfeiçoamento de Pessoal de Nível Superior<br>The raw milk storage under cooling temperatures selects the psychrotropic bacteria growth including species able to produce heat resistant proteases and lipases that are usually associated with milk quality deterioration. The decrease in the cheese production yield is an important problem associated with the psychrotrophic bacteria growth

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9

Kawamoto, Jun. "Studies of cold-adaptation mechanism of a psychrotrophic bacterium, Shewanella livingstonensis Ac10." Kyoto University, 2008. http://hdl.handle.net/2433/136582.

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Kyoto University (京都大学)<br>0048<br>新制・課程博士<br>博士(農学)<br>甲第13493号<br>農博第1670号<br>新制||農||951(附属図書館)<br>学位論文||H20||N4318(農学部図書室)<br>UT51-2007-T869<br>京都大学大学院農学研究科応用生命科学専攻<br>(主査)教授江﨑信芳, 教授清水昌, 教授阪井康能<br>学位規則第4条第1項該当

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10

Park, Jung ha. "Studies of cold-inducible inner membrane proteins of a psychrotrophic bacterium, Shewanella livingstonensis Ac10." Kyoto University, 2012. http://hdl.handle.net/2433/157717.

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Kyoto University (京都大学)<br>0048<br>新制・課程博士<br>博士(農学)<br>甲第16926号<br>農博第1942号<br>新制||農||1001(附属図書館)<br>学位論文||H24||N4687(農学部図書室)<br>29601<br>京都大学大学院農学研究科応用生命科学専攻<br>(主査)教授栗原達夫, 教授喜多恵子, 教授阪井康能<br>学位規則第4条第1項該当

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11

Drevet, Pascal. "Etude du système protéolytique intracellulaire d'une bactérie psychrotrophe du genre Arthrobacter." Grenoble 2 : ANRT, 1987. http://catalogue.bnf.fr/ark:/12148/cb376046391.

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12

Michel, Valérie. "Caracterisation de la reponse aux chocs thermiques d'une bacterie psychrotrophe d'alteration, pseudomonas fragi." Clermont-Ferrand 2, 1996. http://www.theses.fr/1996CLF21813.

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Les effets de chocs thermiques sur la croissance et la synthese proteique ont ete etudies chez pseudomonas fragi, bacterie psychotrophe d'alteration des aliments. Les cinetiques de synthese proteique ont ete caracterisees par autoradiographies des proteines intracellulaires marquees a la methionine-#3#5s et separees en electrophorese bidimensionnelle (e-2d). La croissance se poursuivait avec un taux caracteristique de la temperature finale du choc de facon immediate apres les chocs chauds de 5 a 20 ou 30c et de 28 a 34c, et apres 3 et 5 h de latence a la suite d'un transfert de 20 a 5c et de 3

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13

Dai, Xian-zhu. "Studies on the folding of an outer membrane protein from a psychrotrophic bacterium, Shewanella livingstonensis Ac10." Kyoto University, 2012. http://hdl.handle.net/2433/157718.

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Kyoto University (京都大学)<br>0048<br>新制・課程博士<br>博士(農学)<br>甲第16927号<br>農博第1943号<br>新制||農||1001(附属図書館)<br>学位論文||H24||N4688(農学部図書室)<br>29602<br>京都大学大学院農学研究科応用生命科学専攻<br>(主査)教授栗原達夫, 教授小川順, 教授阪井康能<br>学位規則第4条第1項該当

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14

GRABSKA, JADWIGA. "Contribution a la determination de l'activite des lipases des bacteries psychrotrophes dans le lait cru." Paris, Institut national d'agronomie de Paris Grignon, 1986. http://www.theses.fr/1986INAPA003.

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Le principal objectif etait la recherche et la mise au point d'une methode de determination de l'activite lipasique des laits de collecte permettant de differencier la lipoproteine lipase naturelle et les lipases excretees par les bacteries psychrotrophes, notamment le genre pseudomonas. Ces toutes dernieres annees, plusieurs methodes ont ete proposees dans le but de detecter ces enzymes, le plus souvent presentes en faibles quantites en raison de leur thermoresistance. La complexite du milieu lait, avec la presence de proteines, de matieres grasses et celles d'une autre enzyme lipolytique, re

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15

Girardot, Françoise. "Bases moléculaires de la croissance à basse température chez la bactérie psychrotrophe Arthrobacter globiformis SI55." Lyon 1, 1995. http://www.theses.fr/1995LYO10164.

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La croissance a basse temperature a ete etudiee chez la bacterie psychrotrophe arthrobacter globiformis si55. Elle fait intervenir des proteines particulieres appelees caps (cold acclimation proteins) qui persistent pendant toute la duree de la croissance au froid. D'autres proteines appelees csps (cold shock proteins) sont surexprimees transitoirement apres des chocs froids d'amplitude variee. L'analyse en afc (analyse factorielle des correspondances) de leur cinetique d'induction en fonction de la temperature de choc a permis de degager nettement trois groupes de proteines: un groupe de prot

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16

Sugiura, Miwa. "Effects of eicosapentaenoic acid-containing phospholipids on the formation of membrane proteins from Shewanella livingstonensis Ac10." Kyoto University, 2018. http://hdl.handle.net/2433/235099.

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Kyoto University (京都大学)<br>0048<br>新制・課程博士<br>博士(農学)<br>甲第21379号<br>農博第2303号<br>新制||農||1071(附属図書館)<br>学位論文||H30||N5152(農学部図書室)<br>京都大学大学院農学研究科応用生命科学専攻<br>(主査)教授栗原達夫, 教授植田充美, 教授小川順<br>学位規則第4条第1項該当

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17

Trial, De Greef Nathalie. "Etude de l'effet de l'hydrolyse des caséines par des protéases de bacteries psychrotrophes sur la texture du yoghourt brassé." Montpellier 2, 2002. http://www.theses.fr/2002MON20001.

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18

Felipus, Nadine Cristina. "Impacto do transporte a granel na qualidade microbiológica e físico-química e na composição do leite cru refrigerado em indústria de laticínios." Universidade do Estado de Santa Catarina, 2017. http://tede.udesc.br/handle/handle/2562.

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Submitted by Claudia Rocha (claudia.rocha@udesc.br) on 2018-03-16T12:55:23Z No. of bitstreams: 1 PGCA17MA215.pdf: 1647507 bytes, checksum: 1c4f64e83500f380a07f262ae5ae2919 (MD5)<br>Made available in DSpace on 2018-03-16T12:55:23Z (GMT). No. of bitstreams: 1 PGCA17MA215.pdf: 1647507 bytes, checksum: 1c4f64e83500f380a07f262ae5ae2919 (MD5) Previous issue date: 2017-03-21<br>Milk is nutritionally complete, but a highly perishable food and its characteristics easily altered by the action of microorganisms and by the manipulation to which it is submitted. The quality and shelf life of the product

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19

Reche, Natália Luiza Machado. "Influência do armazenamento do leite em resfriador por expansão direta sobre a contagem de micro-organismos e estabilidade da caseína." Universidade do Estado de Santa Catarina, 2013. http://tede.udesc.br/handle/handle/900.

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Made available in DSpace on 2016-12-08T16:24:16Z (GMT). No. of bitstreams: 1 PGCA13MA125.pdf: 1115012 bytes, checksum: 736f2a82baac7129fc498949399b0b87 (MD5) Previous issue date: 2013-11-28<br>The milk is rich in proteins, lipids, water and carbohydrates, making it an excellent environment for bacterial contamination from different origins and for its proliferation. Bacterial multiplication depends on its capacity of adaptation to the environment and time available. In dairy farms, milk can be storage under cold in bulk tanks models of two and four milking capacity, with a chilling capacity

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20

Decimo, M. "BACTERIAL ENZYMATIC ACTIVITIES AS POTENTIAL MARKERS FOR ASSESSING THE TECHNOLOGICAL PROPERTIES OF (UN)PROCESSED MILK." Doctoral thesis, Università degli Studi di Milano, 2014. http://hdl.handle.net/2434/244558.

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RIASSUNTO I batteri psicrotrofi sono i principali responsabili del deterioramento del latte crudo o termotrattato poiché sono in grado di sintetizzare proteasi e lipasi extracellulari termostabili, causa di formazione di odori e sapori sgradevoli, fenomeni di gelificazione, riduzione delle proprietà schiumogene del latte, perdita di qualità sensoriale e riduzione della shelf-life. Ad oggi, non esiste ancora una sufficiente conoscenza degli specifici pathways proteolitici e lipolitici di questi enzimi termostabili. Inizialmente questo lavoro ha riguardato la caratterizzazione dell’attivit

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21

Liu, Chin-Chun, and 劉芹君. "Isolation of Psychrotrophic Bacteria in the Coastal Area of Shalun, Tamshui." Thesis, 2000. http://ndltd.ncl.edu.tw/handle/13844082927179602188.

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碩士<br>國立陽明大學<br>微生物暨免疫學研究所<br>88<br>In this thesis, we sampled seawater and sediment at the seaside of northern Taiwan and studied several novel microbial strains isolated from the sample. Among all isolates, seven pure strains were picked for further study, and they are designated as strains SR1~SR7 respectively. By using various classification methods, such as morphology, physiological properties, biochemical tests, GC content, and 16S rRNA sequence comparison, strains SR1~SR7 are classified into known categories. Strains SR1~SR7 are all Gram-negative, motile, polar-flagellated, aerobic, rod

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22

Meer, Ralph R. "Identification and characterization of some psychrotrophic heat resistant/sporeforming bacteria in the Grade A raw milk supply of Oregon." Thesis, 1987. http://hdl.handle.net/1957/27089.

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Heat resistant sporeforming psychrotrophic bacteria were isolated from raw milk samples from 59 Grade A farms in Oregon. Forty-nine of the 59 (83%) raw milk samples in this survey contained sporeforming psychrotrophic bacteria; isolates from twenty-four (40%) of the samples exhibited proteolytic properties. Populations of sporeforming psychrotrophic bacteria ranged from <10 to >10,000 CFU/mL for all samples. One hundred-two isolates were identified as Bacillus species. Twelve different Bacillus species were identified with B. licheniformis being the most predominate (18% of the samples) and B.

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23

Sun, Jun-Ning, and 孫君寧. "The biodiversity of psychrotrophic bacteria from the soil of high-elevation regions in Yushan National Park." Thesis, 2017. http://ndltd.ncl.edu.tw/handle/e5bwvm.

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碩士<br>國立臺灣大學<br>海洋研究所<br>105<br>Most of the Earth’s biosphere is permanently cold environment, which temperature is lower than 5 °C, including alpine and polar regions which constitute 25 % of the world’s land surface area, and deep sea, etc. Many psychrotrophs have been isolated from these cold environments. There isn’t any permanently cold environment in Taiwan, for the reason that Taiwan is situated in the subtropical zone. However, the high elevation regions (over 3,000 m) in the Yushan National Park are classified as frigid zones. Therefore, psychrotrophs may abundantly exist in those col

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24

Fu, Ching-Fan, and 傅靜凡. "Isolation Characterization of Psychrotrophic Histamine-producing Bacterium in Seafoods." Thesis, 2013. http://ndltd.ncl.edu.tw/handle/64066131233869518034.

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碩士<br>國立澎湖科技大學<br>食品科學研究所<br>101<br>Abstract Histamine fish poisoning usually results from the ingestion of fish containing high levels of histamine. Histamine is formed in fish by certain bacteria that can decarboxylate histidine. Most of the histamine-producing bacteria are mesophilic, strict temperature control is important in the prevention of histamine fish poisoning. However, the presence of psychrophilic histamine-producing bacteria also causes the accumulation of histamine at low temperatures. To study those psychrophilic histamine-producing bacteria,27 strains were isolated from Scomb

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25

MIKULOVÁ, Magda. "Psychrotrofní lipolytické bakterie a obsah volných mastných kyselin v bazénových vzorcích syrového kravského mléka." Doctoral thesis, 2012. http://www.nusl.cz/ntk/nusl-115843.

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The objective of the study was to analyze the relationship of psychrotrophic lipolytic bacteria in bulk samples of cow?s raw milk and their metabolic activity based on the content of free fatty acids and identifying the factors that may influence their levels. The contents of free fatty acids (FFA) and counts of total bacteria, psychrotrophic lipolytic bacteria (PLiBC) and somatic cells were determined in 150 samples of cow?s bulk raw milk on 20 farms with three different milking technologies in South Bohemia during 2008?10. FFA were determined using an extraction-titration method. Within the

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26

Lee, Yung-Hua, and 李永華. "Identification of gene controlling extracellular protease production from a Psychrotrophic Bacterium Pseudomonas sp. P90." Thesis, 2001. http://ndltd.ncl.edu.tw/handle/84962456987608232763.

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碩士<br>國立中興大學<br>植物學系<br>89<br>Recent resrarch reports that several exo-enzyme activity of genus Pseudomonas were regulated by two-component regulatory system composed of lemA/gacA gene. Pseudomonas sp. P90 which is a psychotrophic bacterium isolated from Ta-Ta-Ja Taiwan in Mountain had both exocellular protease and lipase activity. After mutagenizing with mini transposon pUT-Tn5/(pfm)Tc a strain P90A2 without exocellular protease and lipase activity were isolated. According to the result of Northern blot, the loss of protease activity was due to no transcription of protease gene bu

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CHIUN, CHIU WEI, and 邱偉鈞. "Metalloprotease from the psychrotrophic bacterium Pseudomonas sp. P90:gene cloning and enzyme purification and characterization." Thesis, 2000. http://ndltd.ncl.edu.tw/handle/83297037934669534717.

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碩士<br>國立中興大學<br>植物學系<br>88<br>Abstract Pseudomonas sp. P90, a gram-negative psychrotrophic bacterium we isolated in the Taiwan Mountain Ta-Ta-Ja about 2700 M alt, secretes a thermostable protease into the culture medium. To clone this protease gene, we used PCR to amplify a part of the protease gene. A plasmid carrying this PCR product and ori was integrated via homologous recombination to the chromosome of wild type P90, and then the chromosome was digested with restriction enzyme followed by self-ligation by treatment with T4 DNA ligase. A clone obtained, pZP1, contained a 6.0-kb

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石靜慧. "Cloning and characterization of genes controling extracellular protease production from a psychrotrophic bacterium pseudomonas sp.P90." Thesis, 2003. http://ndltd.ncl.edu.tw/handle/41382392034130884251.

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Huei-Ming, Lin, and 林惠敏. "Analysis and Comparison of Gene Regulating Extracellular Protease Production from a Psychrotrophic Bacterium Pseudomonas sp. P90." Thesis, 2002. http://ndltd.ncl.edu.tw/handle/07643297878005290674.

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碩士<br>國立中興大學<br>植物學系<br>90<br>Prokaryotes can respond to a variety of environmental stimuli by means of systems generally composed of two proteins. The first protein is sensory kinase, which has sensor function and kinase activity. The second protein is response regulator. In the genus Pseudomonas, a conserved two-component regulatory system consisting of the GacS and GacA has a decisive role in the control of the production of extracellular enzymes, antibiotics or toxins. Previously, Pseudomonas sp. P90, a gram-negative psychrotrophic bacterium isolated from Ta-Ta-Ja Mountain of middle Taiwa

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Lee, Hui-Chen, and 李慧貞. "Lipolytic enzyme from the Psychrotrophic Bacterium Pseudomonas sp. P116：Gene Cloning and enzyme Purification and Characterization." Thesis, 2001. http://ndltd.ncl.edu.tw/handle/45086069506887322912.

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碩士<br>國立中興大學<br>植物學系<br>89<br>In this study, Pseudomonas sp. P116, a gram-negative psychrotrophic bacterium displays extracellular lipolytic and proteolytic activities,was isolated from Ho-Huan Mountain of middle Taiwan. Following shotgun cloning, a DNA fragment containg lipolytic enzyme gene was obtained and sequenced. Analysis of the complete nucleotide sequence of this fragment revealed an open reading frame of 930 nucleotids, which may encode a sequence of 310 amino acids. Alignment comparison showed this amino sequence was homologous to the lipase/esterase of Pseudomonas aeruginosas PAO1

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Vithanage, Nuwan R. "Mapping the thermo-tolerant proteases in ultra high temperature (UHT) treated milk using molecular approaches." Thesis, 2017. https://vuir.vu.edu.au/34726/.

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Refrigerated storage of raw milk selects for psychrotrophic bacterial genera such as Pseudomonas (mainly Pseudomonas fluorescens), Bacillus, Acinetobacter, Hafnia, Klebsiella, Rahnella, Stenotrophomonas and Aeromonas, Serratia, which are disseminated by means of feed, faeces, bedding material, soil, air, water and milking and processing equipment at the farming and processing environments. These bacteria produce a wide range of extracellular proteases that are resistant to the current heating regimes used in Ultra High Temperature (UHT) processing, typically 135-150 °C for 2-10 s. The residual

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