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1

Ortiz, Rocío, Mónica Antilén, Hernán Speisky, Margarita E. Aliaga, and Camilo López-Alarcón. "Analytical Parameters of the Microplate-Based ORAC-Pyrogallol Red Assay." Journal of AOAC INTERNATIONAL 94, no. 5 (2011): 1562–66. http://dx.doi.org/10.5740/jaoacint.11-033.

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Abstract The analytical parameters of the microplate-based oxygen radicals absorbance capacity (ORAC) method using pyrogallol red (PGR) as probe (ORAC-PGR) are presented. In addition, the antioxidant capacity of commercial beverages, such as wines, fruit juices, and iced teas, is estimated. A good linearity of the area under the curve (AUC) versus Trolox concentration plots was obtained [AUC = (845 ± 110) + (23 ± 2) [Trolox, μM], R = 0.9961, n = 19]. QC experiments showed better precision and accuracy at the highest Trolox concentration (40 μM) with RSD and REC (recuperation) values of 1.7 and
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2

Singhal, Pallavi, and Hirendra N. Ghosh. "Ultrafast excited state dynamics of S2 and S1 states of triphenylmethane dyes." Phys. Chem. Chem. Phys. 16, no. 31 (2014): 16824–31. http://dx.doi.org/10.1039/c4cp01766h.

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Excited state dynamics of S2 and S1 states for a series of TPM dyes, pyrogallol red (PGR), bromopyrogallol red (Br-PGR) and aurin tricarboxylic acid (ATC), have been monitored by using ultrafast transient absorption and fluorescence up-conversion techniques.
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3

Ortiz, Rocío, Mónica Antilén, Hernán Speisky, Margarita E. Aliaga, Camilo López-Alarcón, and Steve Baugh. "Application of a Microplate-Based ORAC-Pyrogallol Red Assay for the Estimation of Antioxidant Capacity: First Action 2012.03." Journal of AOAC INTERNATIONAL 95, no. 6 (2012): 1558–61. http://dx.doi.org/10.5740/jaoacint.cs2012_03.

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Abstract A method was developed for microplate-based oxygen radicals absorbance capacity (ORAC) using pyrogallol red (PGR) as probe (ORAC-PGR). The method was evaluated for linearity, precision, and accuracy. In addition, the antioxidant capacity of commercial beverages, such as wines, fruit juices, and iced teas, was measured. Linearity of the area under the curve (AUC) versus Trolox® concentration plots was [AUC = (845 ± 110) + (23 ± 2) [Trolox, μM]; R = 0.9961, n = 19]. Analyses showed better precision and accuracy at the highest Trolox concentration (40 μM) with RSD and recovery (REC) valu
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4

Mozgovaya, Elena V., Natalya E. Androsova, and Vladimir V. Dorofeicov. "Compared diagnostic value of proteinuria definition applying different methods at pregnants with preeclampsia." Journal of obstetrics and women's diseases 66, no. 4 (2017): 51–56. http://dx.doi.org/10.17816/jowd66451-56.

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Colorimetric method of protein determination in urine with use of pyrogallol red (PGR) has certain advantages over a routine method of proteinuria determination with use of sulfosalicylic acid (SSA) which is still applied in most clinics in the Russian Federation. For the purpose to determinate the diagnostic significance of proteinuria estimation by different methods at pregnants with preeclampsia, conducted a comparative research of proteinuria levels at 68 pregnants with preeclampsia and at 15 healthy pregnant by two methods: with sulfosalicylic acid and with pyrogallol red. At the same pat
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5

Hasanpour, Foroozan, Hossein Teimori, Masoud Fouladgar, and Masoumeh Taei. "Trace and selective determination of cobalt(II) in water and salt samples using cathodic adsorptive stripping voltammetry in the presence of pyrogallol red." Journal of the Serbian Chemical Society 78, no. 5 (2013): 717–24. http://dx.doi.org/10.2298/jsc120305070h.

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A sensitive and selective procedure is presented for voltammetric determination of cobalt. The procedure involves an adsorptive accumulation of cobalt pyrogallol red (PGR) complex on stationary mercury drop electrode, followed by cathodic stripping voltammetry measurement of reduction current of adsorbed complex at -1.17 V (vs. Ag/AgCl). The optimum conditions for determination of cobalt include pH 11.0, 35 ?M pyrogallol red, an accumulation potential of -0.9 V (vs. Ag/AgCl) and scan rate 80 mVs-1. The peak current is proportional to the concentration of cobalt over the concentration range of
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6

A., B. Zade, P. Kalbende P., B. Upase A., and Belsare G.·w. "Sensitive microspectrophotometric determination ofthorium(IV) and uranium(VI) with pyrogallol red in presence of cationic surfactant." Journal of Indian Chemical Society Vol. 89, Jun 2012 (2012): 811–22. https://doi.org/10.5281/zenodo.5763829.

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Department of Chemistry, Laxminarayan Institute of Technology, R.T.M. Nagpur University, Nagpur-440 010, Maharashtra, India <em>E-mail </em>: ab_zadel8@yahoo.com Department of Chemistry, New English Junior College, Warud, Dist. Amravati-444 906, Maharashtra, India P.G. Department of Chemistry, Shri Shivaji Science College, Akola-444 001, Maharashtra, India Manuscript received 31 May 2011, revised 12 October 2011, accepted 17 October 2011 Formation of water soluble deeply colored ternary complexes of Th<sup>IV</sup> and U<sup>VI</sup> metal ions with pyrogallol red (PGR) in presence of cationic
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7

Jinadasa, Ambulugala Gamage Rajika Greshamali, Lanka Acharige Shalka Madushan Srimantha, Indika Deepani Siriwardhana, Kalani Buddika Gunawardana, and Anoja Priyadarshani Attanayake. "Optimization of 25% Sulfosalicylic Acid Protein-to-Creatinine Ratio for Screening of Low-Grade Proteinuria." International Journal of Analytical Chemistry 2021 (January 28, 2021): 1–9. http://dx.doi.org/10.1155/2021/6688941.

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Proteinuria is an important prognostic marker in the diagnosis and management of kidney diseases. Sulfosalicylic acid method (SSA) is a simple, low cost, qualitative test, widely used to assess proteinuria. The aim of this study was to optimize SSA test as a quantitative screening tool to assess proteinuria at lower excretory levels which would facilitate the screening and early diagnosis of renal impairment using protein-to-creatinine ratio (PCR). The study was conducted in two phases. In phase I, optimum SSA percentage to detect low-grade proteinuria was selected by comparing the performance
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8

Maity, Partha, Tushar Debnath, Uday Chopra, and Hirendra Nath Ghosh. "Cascading electron and hole transfer dynamics in a CdS/CdTe core–shell sensitized with bromo-pyrogallol red (Br-PGR): slow charge recombination in type II regime." Nanoscale 7, no. 6 (2015): 2698–707. http://dx.doi.org/10.1039/c4nr05829a.

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For the first time ultrafast cascading hole and electron transfer dynamics in a CdS/CdTe type II core–shell sensitized with a molecular adsorbate (Br-PGR) have been demonstrated using transient absorption spectroscopy.
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9

Bridi, Raquel, Ady Giordano, Maria Fernanda Peñailillo, and Gloria Montenegro. "Antioxidant Effect of Extracts from Native Chilean Plants on the Lipoperoxidation and Protein Oxidation of Bovine Muscle." Molecules 24, no. 18 (2019): 3264. http://dx.doi.org/10.3390/molecules24183264.

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The present study investigated the antioxidant potential and the ability to inhibit lipid and protein oxidation in bovine meat of four native Chilean species: canelo (Drimys winteri), nalca (Gunnera tinctoria), tiaca (Caldcluvia paniculata), and ulmo (Eucryphia cordifolia). Phenolic acids (gallic, chlorogenic, caffeic, and coumaric) and flavonoids (catechin, epicatechin, and rutin) were identified and quantified by HPLC-MS/MS. Drimys winteri extract exhibited the highest antioxidant capacity evaluated by oxygen radical absorption capacity-red pyrogallol method (ORAC-PGR) and ferric ion reducin
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10

Acosta-Quiroga, Karen, Esteban Rocha-Valderrama, Matías Zúñiga-Bustos, et al. "Gross Antioxidant Capacity and Anti-Inflammatory Potential of Flavonol Oxidation Products: A Combined Experimental and Theoretical Study." Antioxidants 14, no. 4 (2025): 479. https://doi.org/10.3390/antiox14040479.

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This study evaluated the antioxidant capacity of the oxidation products of three flavonols using oxygen radical absorbance capacity—fluorescein assay (ORAC-FL), oxygen radical absorbance capacity—pyrogallol red assay (ORAC-PGR), and the cellular antioxidant activity (CAA) assay in human dermal fibroblast (HFF) cells, with 2,2’-azobis(2-amidinopropane) dihydrochloride (AAPH) as a free radical generator under controlled pH and solvent conditions. At pH 2 in a polar aprotic solvent, BZF-OH (benzofuranone-OH) compounds were formed, while methoxylated analogs were obtained at pH 7 in a polar protic
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11

Waugh, Jason, Richard Hooper, Edmund Lamb, et al. "Spot protein–creatinine ratio and spot albumin–creatinine ratio in the assessment of pre-eclampsia: a diagnostic accuracy study with decision-analytic model-based economic evaluation and acceptability analysis." Health Technology Assessment 21, no. 61 (2017): 1–90. http://dx.doi.org/10.3310/hta21610.

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Background The National Institute for Health and Care Excellence (NICE) guidelines highlighted the need for ‘large, high-quality prospective studies comparing the various methods of measuring proteinuria in women with new-onset hypertensive disorders during pregnancy’. Objectives The primary objective was to evaluate quantitative assessments of spot protein–creatinine ratio (SPCR) and spot albumin–creatinine ratio (SACR) in predicting severe pre-eclampsia (PE) compared with 24-hour urine protein measurement. The secondary objectives were to investigate interlaboratory assay variation, to evalu
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12

Orsonneau, J. L., P. Douet, C. Massoubre, P. Lustenberger, and S. Bernard. "An improved pyrogallol red-molybdate method for determining total urinary protein." Clinical Chemistry 35, no. 11 (1989): 2233–36. http://dx.doi.org/10.1093/clinchem/35.11.2233.

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Abstract We adapted the pyrogallol red-molybdate method for total urinary protein to the Cobas Bio centrifugal analyzer. The method is simple, rapid, sensitive, and inexpensive. Addition of 25 mg of sodium dodecyl sulfate per liter to the reagent modifies protein reactivities so that the chromogenicity of human gamma globulins is the same as that of albumin. Results by this method and a comparison method that included gel filtration and a modified biuret reaction correlated well (r = 0.951).
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13

Paudel, Govinda Akela, Saroj Kunwar, Shrijan Shakya, Rasana Shakya, Arbin Baral, and Kailash Shrestha. "Spot Urine Protein Creatinine Ratio in Comparison to 24-Hour Urinary Protein for Detection of Proteinuria in Type 2 Diabetic Patients." Med Phoenix 9, no. 1 (2024): 24–28. http://dx.doi.org/10.3126/medphoenix.v9i1.67181.

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Introduction: Diabetic nephropathy is a disease in which both glucose and protein appears in urine and is characterized by progressive renal damage due to the long-term hyperglycemia affecting the glomerulus and ultimately degrading the filtrating ability of the kidney. Determination of 24-hour urine protein excretion is simple and inoffensive method and is considered to be a gold-standard one. The method is time consuming, tiresome, and imprecise due to collection error. Random urine sample for protein creatinine ratio (PCR) would be more acceptable and less time consuming. Therefore, the pre
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14

Triviño, Juan José, Miquel Esteban, José Díaz-Cruz, Nuria Serrano, Alejandro Alvarez-Lueje, and VERONICA ARANCIBIA. "Determination of Chromium(VI) with Screen‐Printed ex‐situ Bismuth film electrodes by adsorptive stripping voltammetry." Electroanalysis, September 12, 2024. http://dx.doi.org/10.1002/elan.202400218.

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This work presents a fast and sensitive method, with an ex−situ bismuth film screen‐printed carbon electrode (ex−situ Bi−SPCE), for the determination of Cr(VI) through adsorptive stripping voltammetry (AdSV) using Pyrogallol Red (PGR) as complexing agent. The method is based on the oxidation of PGR by Cr(VI) and posterior complexation with Cr(III). To obtain a sensitive and selective method, the effects of various parameters such as pH, ligand concentration of PGR (C &lt;sub&gt;PGR&lt;/sub&gt;), accumulation potential, and accumulation time (E &lt;sub&gt;acc&lt;/sub&gt;, t &lt;sub&gt;acc&lt;/s
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15

Koide, Rintaro, Hiroyuki Ikeda, Satoko Takahashi, et al. "Difference in urinary protein‐to‐creatinine ratio using the benzethonium chloride and pyrogallol red methods in children." Pediatrics International 66, no. 1 (2024). http://dx.doi.org/10.1111/ped.15813.

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AbstractBackgroundThe appropriate reference value of the urinary protein‐to‐creatinine ratio (PCR) for proteinuria may change when the urinary pyrogallol red (PR) protein assay method is changed to the benzethonium chloride method (BC). This study aimed to evaluate the difference between BC‐based PCR (BC‐PCR) and PR‐based PCR (PR‐PCR) values in children.MethodsWe compared the BC‐PCR and PR‐PCR values in the same first‐morning urine samples without significant proteinuria in school urine screening settings. The upper limit of the reference values was set at the 97.5th percentile.ResultsNotably,
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16

Adu, O. B., G. A. Adeyemo, O. B. Falua, et al. "The Effect of Thaumatococcus danielli Leaf Extracts on Immunological and Oxidative Stress Markers in Rat." Asian Journal of Biochemistry, Genetics and Molecular Biology, April 22, 2021, 6–14. http://dx.doi.org/10.9734/ajbgmb/2021/v7i430179.

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This work demonstrated the immunological and antioxidant activities of Thaumatococcus daniellii leaf extracts and their potential as the pharmaceutical factor for the treatment of some pathological disorders such as cancer, leucopenia, cells inflammation, dietary, atherosclerosis, neurodegenerative disease and other immunological and oxidative stress disorders.36 albino rats of average weight 140 ± 5 g were randomly grouped into 6 with six rats per group. The rats in group A (positive control) received only 0.1% DMSO. The rats in the negative control (group B) received pyrogallol (immunosuppre
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