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1

Dowd, S., A. A. Sneddon, and S. M. Keyse. "Isolation of the human genes encoding the pyst1 and Pyst2 phosphatases: characterisation of Pyst2 as a cytosolic dual-specificity MAP kinase phosphatase and its catalytic activation by both MAP and SAP kinases." Journal of Cell Science 111, no. 22 (November 15, 1998): 3389–99. http://dx.doi.org/10.1242/jcs.111.22.3389.

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We have isolated the human genes encoding the Pyst1 (MKP-3) and Pyst2 (MKP-X) MAP kinase phosphatases. Both genes consist of three exons interrupted by two introns and lack an intron which is conserved in all the other members of this gene family characterised to date. This reinforces the conclusion that Pyst1 and Pyst2 are members of a distinct and structurally homologous subfamily of dual-specificity (Thr/Tyr) MAP kinase phosphatases. We find that Pyst2 mRNA is constitutively expressed in a wide variety of human cell lines including those derived from ovarian, bladder and breast cancers. While there is no evidence for inducible expression of Pyst2 mRNA in human skin fibroblasts in response to cellular stress, Pyst2 mRNA levels are moderately increased in response to serum stimulation. Pyst2 protein is predominantly cytosolic when expressed in COS-1 cells. In common with Pyst1, Pyst2 shows substrate selectivity for the classical p42 (ERK2) isoform of MAP kinase both in vitro and in vivo, displaying much reduced activity towards stress activated MAP kinase isoforms such as JNK-1 and p38/RK. Pyst2 binds p42 MAP kinase in vivo and both MAP kinase binding and substrate selectivity correlate with the ability of different recombinant MAP and SAP kinases to cause catalytic activation of the Pyst2 phosphatase in vitro.
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2

Deng, Ji-Hua, Gui-Quan Guo, and Di-Chang Zhong. "Bis[N-aminocarbonyl-N′-(3-pyridylmethylene-κN)hydrazine]diaquabis(thiocyanato-κN)zinc(II)." Acta Crystallographica Section E Structure Reports Online 63, no. 11 (October 10, 2007): m2696—m2697. http://dx.doi.org/10.1107/s1600536807048908.

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The Zn atom of the title complex, [Zn(NCS)2(C7H8N4O)2(H2O)2] or [Zn(SCN)2(H-Pysc)2(H2O)2] [H-Pysc = N-aminocarbonyl-N′-(3-pyridylmethylene)hydrazine], derived from the condensation of pyridine-3-carbaldehyde and semicarbazone, is located at a crystallographic centre of inversion and is octahedrally coordinated by two thiocyanate anions, two aqua molecules and two molecules of the neutral Schiff base ligand H-Pysc. The Schiff base molecules act as monodentate ligands coordinating the metal through the pyridyl N atom, whereas the amide O and imine N atoms remain uncoordinated. The crystal packing is stabilized by intermolecular hydrogen bonds involving H-Pysc ligands, thiocyanate anions and water molecules.
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3

Val, D. L., A. Chapman-Smith, M. E. Walker, J. E. Cronan, and J. C. Wallace. "Polymorphism of the yeast pyruvate carboxylase 2 gene and protein: effects on protein biotinylation." Biochemical Journal 312, no. 3 (December 15, 1995): 817–25. http://dx.doi.org/10.1042/bj3120817.

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In Saccharomyces cerevisiae there are two isoenzymes of pyruvate carboxylase (Pyc) encoded by separate genes designated PYC1 and PYC2. We report the isolation and sequencing of a PYC2 gene, and the localization of both genes on the physical map of S. cerevisiae. Comparison with the previously reported sequence [Stucka, Dequin, Salmon and Gancedo (1991) Mol. Gen. Genet. 229, 307-315] revealed significant differences within the open reading frame. The most notable difference was near the 3′ end, where we found a single base deletion reducing the open reading frame by 15 bases. We have confirmed the C-terminus of Pyc2 encoded by the gene isolated here by expressing and purifying an 86-amino-acid biotin-domain peptide. In addition, we investigated the effects of the two changes in the Pyc2 biotin domain (K1155R substitution and Q1178P/five-amino-acid extension) on the extent of biotinylation in vivo by Escherichia coli biotin ligase, and compared the biotinylation of peptides containing these changes with that of two different-length Pyc1 biotin-domain peptides. The K1155R substitution had very little effect on biotinylation, but the five-amino-acid C-terminal extension to Pyc2 and the N-terminal extension to Pycl both improved biotinylation in vivo.
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4

Yu, Zhaojun, Peng Jiang, and Yanmei Chen. "Crystal structure of poly[[hexaqua-1κ4O,2κ2O-bis(μ3-pyridine-2,4-dicarboxylato-1κO2:2κ2N,O2′;1′κO4)cobalt(II)strontium(II)] dihydrate]." Acta Crystallographica Section E Crystallographic Communications 71, no. 9 (August 15, 2015): m167—m168. http://dx.doi.org/10.1107/s2056989015014942.

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In the title polymeric complex, {[CoSr(C7H3NO4)2(H2O)6]·2H2O}n, the CoIIion, which is situated on a crystallographic centre of inversion, is six-coordinated by two O atoms and two N atoms from two pyridine-2,4-dicarboxylate (pydc2−) ligands and two terminal water molecules in a slightly distorted octahedral geometry, to form atrans-[Co(pydc)2(H2O)2]2−unit. The SrIIion, situated on aC2axis, is coordinated by four O atoms from four pydc2−ligands and four water molecules. The coordination geometry of the SrIIatom can be best described as a distorted dodecahedron. Each SrIIion bridges four [Co(pydc)2(H2O)2]2−units by four COO−groups of four pydc2−ligands to form a three-dimensional network structure. Two additional solvent water molecules are observed in the crystal structure and are connected to the three-dimensional coordination polymer by O—H...O hydrogen bonds. Further intra- and intermolecular O—H...O hydrogen bonds consolidate the overall structure.
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5

Yang, Yongfeng, Tao Li, and Yanmei Chen. "The first three-dimensional FeIII–SrIIheterometallic coordination polymer: poly[[diaquatetrakis(μ3-pyridine-2,3-dicarboxylato)diiron(III)strontium(II)] dihydrate]." Acta Crystallographica Section C Structural Chemistry 71, no. 10 (September 18, 2015): 903–7. http://dx.doi.org/10.1107/s2053229615016617.

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The title compound, poly[[diaqua-1κ2O-tetrakis(μ3-pyridine-2,3-dicarboxylato)-2:1:2′κ10N,O2:O2′,O3:O3′;2:1:2′κ8O3:O3′:N,O2-diiron(III)strontium(II)] dihydrate], {[Fe2Sr(C7H3O4)4(H2O)2]·2H2O}n, which has triclinic (P\overline{1}) symmetry, was prepared by the reaction of pyridine-2,3-dicarboxylic acid, SrCl2·6H2O and Fe(OAc)2(OH) (OAc is acetate) in the presence of imidazole in water at 363 K. In the crystal structure, the pyridine-2,3-dicarboxylate (pydc2−) ligand exhibits μ3-η1,η1:η1:η1and μ3-η1,η1:η1,η1:η1coordination modes, bridging two FeIIIcations and one SrIIcation. The SrIIcation, which is located on an inversion centre, is eight-coordinated by six O atoms of four pydc2−ligands and two water molecules. The coordination geometry of the SrIIcation can be best described as distorted dodecahedral. The FeIIIcation is six-coordinated by O and N atoms of four pydc2−ligands in a slightly distorted octahedral geometry. Each FeIIIcation bridges two neighbouring FeIIIcations to form a one-dimensional [Fe2(pydc)4]nchain. The chains are connected by SrIIcations to form a three-dimensional framework. The topology type of this framework istfj. The structure displays O—H...O and C—H...O hydrogen bonding.
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6

Sun, Qiming, Xing Zhang, Samragni Banerjee, Peng Bao, Marc Barbry, Nick S. Blunt, Nikolay A. Bogdanov, et al. "Recent developments in the PySCF program package." Journal of Chemical Physics 153, no. 2 (July 14, 2020): 024109. http://dx.doi.org/10.1063/5.0006074.

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7

Von Poelhsitz, G., B. L. Rodrigues, and A. A. Batista. "Partially oxidized ruthenium phosphine thiolates: [Ru(pySO2)0.33(pyS)1.67(dppe)] and [Ru(pySO2)0.355(pyS)1.645(dppp)]." Acta Crystallographica Section C Crystal Structure Communications 62, no. 9 (August 23, 2006): m424—m427. http://dx.doi.org/10.1107/s0108270106027491.

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8

Yousefi, Zakieh, Hossein Eshtiagh-Hosseini, Alireza Salimi, and Janet Soleimannejad. "(2-Aminopyrimidine-κN1)aqua(pyridine-2,6-dicarboxylato-κ3O2,N,O6)copper(II): X-ray and DFT calculated structure." Acta Crystallographica Section C Structural Chemistry 71, no. 5 (April 15, 2015): 386–93. http://dx.doi.org/10.1107/s2053229615005331.

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In the title compound, [Cu(C7H3N2O4)(C4H5N2)(H2O)], (I), pyridine-2,6-dicarboxylate (pydc2−), 2-aminopyrimidine and aqua ligands coordinate the CuIIcentre through two N atoms, two carboxylate O atoms and one water O atom, respectively, to give a nominally distorted square-pyramidal coordination geometry, a common arrangement for copper complexes containing the pydc2−ligand. Because of the presence of Cu...Xbridgedcontacts (X= N or O) between adjacent molecules in the crystal structures of (I) and three analogous previously reported compounds, and the corresponding uncertainty about the effective coordination number of the CuIIcentre, density functional theory (DFT) calculations were used to elucidate the degree of covalency in these contacts. The calculated Wiberg and Mayer bond-order indices reveal that the Cu...O contact can be considered as a coordination bond, whereas the amine group forming a Cu...N contact is not an effective participant in the coordination environment.
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9

Herzberg, Wiebke, and Kolja Glogowski. "Pysca – Automated frequency extraction from photometric time series." Proceedings of the International Astronomical Union 9, S301 (August 2013): 421–22. http://dx.doi.org/10.1017/s1743921313014853.

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10

Levy-Nissenbaum, Orlev, Shlomit Ben-Menachem, Orit Sagi-Assif, and Isaac P. Witz. "The Pyst2-L phosphatase is involved in cell-crowding." Immunology Letters 104, no. 1-2 (April 2006): 138–45. http://dx.doi.org/10.1016/j.imlet.2005.11.013.

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11

Lucas, Soizick, Laurent Toffin, Yvan Zivanovic, Daniel Charlier, Hélène Moussard, Patrick Forterre, Daniel Prieur, and Gaël Erauso. "Construction of a Shuttle Vector for, and Spheroplast Transformation of, the Hyperthermophilic Archaeon Pyrococcus abyssi." Applied and Environmental Microbiology 68, no. 11 (November 2002): 5528–36. http://dx.doi.org/10.1128/aem.68.11.5528-5536.2002.

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ABSTRACT Our understanding of the genetics of species of the best-studied hyperthermophilic archaea, Pyrococcus spp., is presently limited by the lack of suitable genetic tools, such as a stable cloning vector and the ability to select individual transformants on plates. Here we describe the development of a reliable host-vector system for the hyperthermophilic archaeon Pyrococcus abyssi. Shuttle vectors were constructed based on the endogenous plasmid pGT5 from P. abyssi strain GE5 and the bacterial vector pLitmus38. As no antibiotic resistance marker is currently available for Pyrococcus spp., we generated a selectable auxotrophic marker. Uracil auxotrophs resistant to 5-fluoorotic acid were isolated from P. abyssi strain GE9 (devoid of pGT5). Genetic analysis of these mutants revealed mutations in the pyrE and/or pyrF genes, encoding key enzymes of the pyrimidine biosynthetic pathway. Two pyrE mutants exhibiting low reversion rates were retained for complementation experiments. For that purpose, the pyrE gene, encoding orotate phosphoribosyltransferase (OPRTase) of the thermoacidophilic crenarchaeote Sulfolobus acidocaldarius, was introduced into the pGT5-based vector, giving rise to pYS2. With a polyethylene glycol-spheroplast method, we could reproducibly transform P. abyssi GE9 pyrE mutants to prototrophy, though with low frequency (102 to 103 transformants per μg of pYS2 plasmid DNA). Transformants did grow as well as the wild type on minimal medium without uracil and showed comparable OPRTase activity. Vector pYS2 proved to be very stable and was maintained at high copy number under selective conditions in both Escherichia coli and P. abyssi.
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12

Han, Lizhi, Longyi Jin, Enbo Wang, and Zhongmin Su. "Synthesis and characterization of two isostructural 3d–4f coordination compounds based on pyridine-2,6-dicarboxylic acid and 4,4′-bipyridine." Acta Crystallographica Section C Structural Chemistry 75, no. 6 (May 20, 2019): 723–27. http://dx.doi.org/10.1107/s2053229619006004.

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The design and synthesis of 3d–4f heterometallic coordination polymers have attracted much interest due to the intriguing diversity of their architectures and topologies. Pyridine-2,6-dicarboxylic acid (H2pydc) has a versatile coordination mode and has been used to construct multinuclear and heterometallic compounds. Two isostructural centrosymmetric 3d–4f coordination compounds constructed from pyridine-2,6-dicarboxylic acid and 4,4′-bipyridine (bpy), namely 4,4′-bipyridine-1,1′-diium diaquabis(μ2-pyridine-2,6-dicarboxylato)tetrakis(pyridine-2,6-dicarboxylato)bis[4-(pyridin-4-yl)pyridinium]cobalt(II)dieuropium(III) octahydrate, (C10H10N2)[CoEu2(C10H9N2)2(C7H3NO4)6(H2O)2]·8H2O, (I), and 4,4′-bipyridine-1,1′-diium diaquabis(μ2-pyridine-2,6-dicarboxylato)tetrakis(pyridine-2,6-dicarboxylato)bis[4-(pyridin-4-yl)pyridinium]cobalt(II)diterbium(III) octahydrate, (C10H10N2)[CoTb2(C10H9N2)2(C7H3NO4)6(H2O)2]·8H2O, (II), were synthesized under hydrothermal conditions and characterized by IR and fluorescence spectroscopy, thermogravimetric analysis and powder X-ray diffraction. Both compounds crystallize in the triclinic space group P\overline{1}. The EuIII and TbIII cations adopt nine-coordinated distorted tricapped trigonal–prismatic geometries bridged by three pydc2− ligands. The CoII cation has a six-coordination environment formed by two pydc2− ligands, two bpy ligands and two coordinated water molecules. Adjacent molecules are connected by π–π stacking interactions to form a one-dimensional chain, which is further extended into a three-dimensional supramolecular network by multipoint hydrogen bonds.
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13

Levy-Nissenbaum, Orlev, Orit Sagi-Assif, Pia Raanani, Abraham Avigdor, Isaac Ben-Bassat, and Isaac P. Witz. "Overexpression of the dual-specificity MAPK phosphatase PYST2 in acute leukaemia." Cancer Letters 199, no. 2 (September 2003): 185–92. http://dx.doi.org/10.1016/s0304-3835(03)00352-5.

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14

Rakotomamonjy, Jennifer, Lauren Rylaarsdam, and Alicia Guemez-Gamboa. "PYRC2-Related Hypomyelinating Leukodystrophy: More to This Than Meets the Eye." Neuron 107, no. 1 (July 2020): 3–5. http://dx.doi.org/10.1016/j.neuron.2020.06.007.

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15

Dennis, Kristine, Ken Liu, Young-Mi Go, and Dean Jones. "Impact of Food Processing on Concentrations of Metal-Binding Phytochelatins in Plant-Based Food." Current Developments in Nutrition 4, Supplement_2 (May 29, 2020): 748. http://dx.doi.org/10.1093/cdn/nzaa052_017.

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Abstract Objectives Phytochelatins (PyCs), plant-derived metal-binding compounds, are widely found in plants and thought to impact absorption of metals. Our objective was to assess the impact of food processing on PyC concentrations in a set of commonly consumed plant foods in the U.S. population. Methods Plant food types were selected using USDA's Food Consumption data, purchased from local grocery stores, and selected to ensure a variety of processing levels including canned, frozen, and fresh. Carrot, corn, potato, spinach, tomato and pea samples were ground, extracted, and analyzed using an optimized LC-MS/MS method for PyC detection. PyC concentrations were calculated using single-point calibration with authentic standards. Quantifiable PyCs were compared using Student's t-test within each food type by processing level (e.g., canned vs. fresh). Additional comparisons of processing categories were completed when sample size allowed (minimum n = 3 per processing category). Results PyC2-Gly levels were lower in canned versus frozen or fresh carrots, corn, and peas but not potatoes, spinach or tomatoes (carrots: 1.92 ± 1.39 vs. 8.26 ± 1.43; corn: 1.01 ± 0.73 vs. 9.39 ± 2.88; peas: 0.46 ± 0.09 vs. 1.46 ± 0.19 µg/g fresh weight; mean ± SEM, Student's t-test, P < 0.05). In subanalyses of peas (canned vs. fresh; canned vs. frozen; frozen vs. fresh), PyC2-Gly concentrations differed (canned: 0.46 ± 0.09; frozen: 1.15 ± 0.16; fresh: 1.89 ± 0.20 µg/g fresh weight; P < 0.05). Of the four foods with quantifiable PyC3-Gly (corn, potato, tomato, peas), only corn had lower PyC3-Gly levels in flour or canned versus frozen or fresh (0.0014 ± 0.0011 vs. 0.45 ± 0.19 µg/g fresh weight). PyC2-Ala was quantifiable in peas and corn but did not differ by processing level. Conclusions PyC levels were different by level of processing in some foods with higher PyC concentrations in less processed (i.e., fresh or frozen) foods. Evidence suggests PyCs may protect from absorption of dietary toxic metals such as cadmium. Dietary patterns emphasizing less processed plant foods may contain higher PyC concentrations and offer protection from toxic metals present in the diet. Funding Sources National Institute of Environmental Health Sciences.
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16

Flores, Carmen-Lisset, and Carlos Gancedo. "Yarrowia lipolytica Mutants Devoid of Pyruvate Carboxylase Activity Show an Unusual Growth Phenotype." Eukaryotic Cell 4, no. 2 (February 2005): 356–64. http://dx.doi.org/10.1128/ec.4.2.356-364.2005.

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ABSTRACT We have cloned and characterized the gene PYC1, encoding the unique pyruvate carboxylase in the dimorphic yeast Yarrowia lipolytica. The protein putatively encoded by the cDNA has a length of 1,192 amino acids and shows around 70% identity with pyruvate carboxylases from other organisms. The corresponding genomic DNA possesses an intron of 269 bp located 133 bp downstream of the starting ATG. In the branch motif of the intron, the sequence CCCTAAC, not previously found at this place in spliceosomal introns of Y. lipolytica, was uncovered. Disruption of the PYC1 gene from Y. lipolytica did not abolish growth in glucose-ammonium medium, as is the case in other eukaryotic microorganisms. This unusual growth phenotype was due to an incomplete glucose repression of the function of the glyoxylate cycle, as shown by the lack of growth in that medium of double pyc1 icl1 mutants lacking both pyruvate carboxylase and isocitrate lyase activity. These mutants grew when glutamate, aspartate, or Casamino Acids were added to the glucose-ammonium medium. The cDNA from the Y. lipolytica PYC1 gene complemented the growth defect of a Saccharomyces cerevisiae pyc1 pyc2 mutant, but introduction of either the S. cerevisiae PYC1 or PYC2 gene into Y. lipolytica did not result in detectable pyruvate carboxylase activity or in growth on glucose-ammonium of a Y. lipolytica pyc1 icl1 double mutant.
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17

Lountos, George T., Brian P. Austin, Joseph E. Tropea, and David S. Waugh. "Structure of human dual-specificity phosphatase 7, a potential cancer drug target." Acta Crystallographica Section F Structural Biology Communications 71, no. 6 (May 20, 2015): 650–56. http://dx.doi.org/10.1107/s2053230x1500504x.

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Human dual-specificity phosphatase 7 (DUSP7/Pyst2) is a 320-residue protein that belongs to the mitogen-activated protein kinase phosphatase (MKP) subfamily of dual-specificity phosphatases. Although its precise biological function is still not fully understood, previous reports have demonstrated that DUSP7 is overexpressed in myeloid leukemia and other malignancies. Therefore, there is interest in developing DUSP7 inhibitors as potential therapeutic agents, especially for cancer. Here, the purification, crystallization and structure determination of the catalytic domain of DUSP7 (Ser141–Ser289/C232S) at 1.67 Å resolution are reported. The structure described here provides a starting point for structure-assisted inhibitor-design efforts and adds to the growing knowledge base of three-dimensional structures of the dual-specificity phosphatase family.
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18

Harsha, Harsha, Jitendra Kumar Meena, Ram Bhajan, Usha Pant, and Mohammed Talha. "Assessment of genetic diversity using DNA markers among Brassica rapa var. yellow sarson germplasm lines collected from Eastern Uttar Pradesh and Uttarakhand hills." Journal of Applied and Natural Science 8, no. 3 (September 1, 2016): 1333–40. http://dx.doi.org/10.31018/jans.v8i3.963.

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The genetic diversity and the relatedness among thirty-one germplasm lines of yellow sarson collected from eastern UP were evaluated using morphological characters and Random Amplified Polymorphic DNA (RAPD) markers. Molecular parameters, viz. A total number of bands, average polymorphic band, average percent polymorphism, average polymorphic information content (PIC), Jaccard’s similarity coefficient, Principal Coordinate Analysis (PCA) and dendrogram generated using RAPD markers. A total of 148 different polymorphic amplification products were obtained using 10 selected decamer primers. The Jaccard similarity coefficient ranged from 0.557-0.899. Maximum polymorphism detected was 100 %.The range of amplification was from 190bp to 9 kb. Some unique bands were also reported with different primers that can be used for the identification of particular accession. PYSC-11-11 and PYSC-11-36 genotypes showed a maximum number of unique loci of different size. 31 germplasm lines grouped into two major clusters I and II based on RAPD profiling. Morphological characterization was done on the basis of leaf, petal and beak characteristics. The similarity value among the germplasm lines ranged from 0.222 to 1.000 using morphological descriptors. The dendrogram generated grouped the germplasm accession into two major groups at 44% similarity value. The cluster analysis was comparable up to some extent with Principal Coordinate Analysis (PCA) of two and three-dimensional plots. The variability revealed by morphological and molecular profile were found to be non-comparable. This study indicated the presence of high genetic diversity among collected yellow sarson germplasm, which could be used for developing for breeding and germplasm management purposes.
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Park, Hwang-Seo, Jeong-Yi Jeon, Seong-Eon Ryu, and Seung-Jun Kim. "Discovery of Novel Inhibitors of Dual-Specificity Phosphatase Pyst2 with Structure-Based Virtual Screening." Bulletin of the Korean Chemical Society 32, no. 7 (July 20, 2011): 2167–68. http://dx.doi.org/10.5012/bkcs.2011.32.7.2167.

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Wang, Kangkang, Rui Wen, Shuangzhu Wang, Luyang Tian, Junhua Xiao, and Qing Meng. "The molecular structure of novel pyriform spidroin (PySp2) reveals extremely complex central repetitive region." International Journal of Biological Macromolecules 145 (February 2020): 437–44. http://dx.doi.org/10.1016/j.ijbiomac.2019.12.027.

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Ge, Jing-Yuan, Peng Wang, Jian-Ping Ma, Qi-Kui Liu, and Yu-Bin Dong. "A well-resolved cyclic water tetramer in a dinuclear CuIIcoordination complex based on 1,2-bis(pyridin-3-yloxy)ethane and capped by pyridine-2,6-dicarboxylic acid." Acta Crystallographica Section C Crystal Structure Communications 69, no. 11 (October 24, 2013): 1362–66. http://dx.doi.org/10.1107/s0108270113027996.

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μ-1,2-Bis(pyridin-3-yloxy)ethane-κ2N:N′-bis[aqua(pyridine-2,6-dicarboxylato-κ3O2,N,O6)copper(II)] tetrahydrate, [Cu2(C7H3NO4)2(C12H12N2O2)(H2O)2]·4H2O, (I), is a C-shaped molecule based on 1,2-bis(pyridin-3-yloxy)ethane (L) and CuIIin the presence of pyridine-2,6-dicarboxylic acid (H2pydc). The two five-coordinated CuIIcentres are chelated by terminal pydc2−ligands and bridged by anLspacer. The molecules are arranged in a two-dimensional sheetvia15 O—H...O hydrogen bonds, and C—H...O interactions further bridge neighbouring sheets into a three-dimensional supermolecular architecture. The structure includes a well-resolved cyclic water tetramer, which acts as a subunit to form a larger aggregate. A thermogravimetric analysis of complex (I) was also carried out.
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Soleimannejad, Janet, Zohreh Derikvand, and Farzaneh Koleiae. "A new three-dimensional coordination polymer of SrIIbased on dipicolinic acid, with different coordination environments for SrII." Acta Crystallographica Section C Structural Chemistry 70, no. 6 (May 23, 2014): 613–16. http://dx.doi.org/10.1107/s2053229614009668.

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A three-dimensional coordination polymer of SrIIbased on dipicolinic acid (pydcH2) has been synthesized and characterized, namely poly[[diaquabis(μ3-6-carboxypyridine-2-carboxylato)bis(μ4-pyridine-2,6-dicarboxylato)tristrontium(II)] dihydrate], {[Sr3(C7H3NO4)2(C7H4NO4)2(H2O)2]·2H2O}n. The asymmetric unit consists of two unique SrIIcentres (one of them situated on an inversion centre), two independent pydc2−ligands, and one coordinated and one uncoordinated water molecule. The two independent SrIIcations are surrounded by water and dipicolinate molecules in distorted square-antiprism and distorted tricapped trigonal prismatic geometries. The dipicolinate ligands adopt μ3- and μ4-bridging modes, linking the alkaline earth metal centres into a three-dimensional coordination framework. One dipicolinate ligand is doubly deprotonated, while the other is singly deprotonated.
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23

Yang, Eun Ju, and Hae Choon Chang. "Analysis of pYC2, a cryptic plasmid in Lactobacillus sakei BM5 isolated from kimchi." Biotechnology Letters 31, no. 1 (September 18, 2008): 123–30. http://dx.doi.org/10.1007/s10529-008-9842-y.

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Yang, Eun Ju, and Hae Choon Chang. "Construction and evaluation of shuttle vector, pGYC4α, based on pYC2 from Lactobacillus sakei." Biotechnology Letters 33, no. 3 (November 12, 2010): 599–605. http://dx.doi.org/10.1007/s10529-010-0467-6.

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Calvín, Pablo, Juan J. Villalaín, Antonio M. Casas-Sainz, Lisa Tauxe, and Sara Torres-López. "pySCu: A new python code for analyzing remagnetizations directions by means of small circle utilities." Computers & Geosciences 109 (December 2017): 32–42. http://dx.doi.org/10.1016/j.cageo.2017.07.002.

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Park, Hwangseo, Jeong-Yi Jeon, Seong Eon Ryu, and Seung Jun Kim. "ChemInform Abstract: Discovery of Novel Inhibitors of Dual-Specificity Phosphatase Pyst2 with Structure-Based Virtual Screening." ChemInform 42, no. 46 (October 20, 2011): no. http://dx.doi.org/10.1002/chin.201146132.

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Levy-Nissenbaum, Orlev, Orit Sagi-Assif, Dina Kapon, Shay Hantisteanu, Tamar Burg, Pia Raanani, Abraham Avigdor, Isaac Ben-Bassat, and Isaac P. Witz. "Dual-specificity phosphatase Pyst2-L is constitutively highly expressed in myeloid leukemia and other malignant cells." Oncogene 22, no. 48 (October 2003): 7649–60. http://dx.doi.org/10.1038/sj.onc.1206971.

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Orlev, Levy-Nissenbaum, Barak Ehud, Burg-Golani Tamar, Sagi-Assif Orit, Kloog Yoel, and Isaac P. Witz. "Does the dual-specificity MAPK phosphatase Pyst2-L lead a monogamous relationship with the Erk2 protein?" Immunology Letters 92, no. 1-2 (March 2004): 149–56. http://dx.doi.org/10.1016/j.imlet.2003.11.024.

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Maupin, Oliver G., Andrew D. Baczewski, Peter J. Love, and Andrew J. Landahl. "Variational Quantum Chemistry Programs in JaqalPaq." Entropy 23, no. 6 (May 24, 2021): 657. http://dx.doi.org/10.3390/e23060657.

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We present example quantum chemistry programs written with JaqalPaq, a python meta-programming language used to code in Jaqal (Just Another Quantum Assembly Language). These JaqalPaq algorithms are intended to be run on the Quantum Scientific Computing Open User Testbed (QSCOUT) platform at Sandia National Laboratories. Our exemplars use the variational quantum eigensolver (VQE) quantum algorithm to compute the ground state energies of the H2, HeH+, and LiH molecules. Since the exemplars focus on how to program in JaqalPaq, the calculations of the second-quantized Hamiltonians are performed with the PySCF python package, and the mappings of the fermions to qubits are obtained from the OpenFermion python package. Using the emulator functionality of JaqalPaq, we emulate how these exemplars would be executed on an error-free QSCOUT platform and compare the emulated computation of the bond-dissociation curves for these molecules with their exact forms within the relevant basis.
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Sellmann, Dieter, Kinga Hein, and Frank W. Heinemann. "Ruthenium(II) and Ruthenium(III) Complexes Containing the [pyS4]2− Ligand [pyS42− = 2,6-Bis(2-mercaptophenylthio)dimethylpyridine(2−)]." European Journal of Inorganic Chemistry 2004, no. 15 (August 2004): 3136–46. http://dx.doi.org/10.1002/ejic.200400066.

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Sellmann, Dieter, Nicole Blum, and Frank W. Heinemann. "Transition Metal Complexes with Sulphur Ligands, Part 151 [1]. Ligand Enforced Configurations and Low-Spin States of [FeNS4] Cores in [FeII(L)('pyS4')] Complexes with σ and σ-π Ligands (L = N2H4, pyridine, PMe3, PnPr3; 'pyS4'2- = 2,6-bis(2-mercaptophenylthiomethyl)pyridine (2-))." Zeitschrift für Naturforschung B 56, no. 7 (July 1, 2001): 581–88. http://dx.doi.org/10.1515/znb-2001-0704.

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The reactions of [Fe('pyS4')]2 with PMe3 , PnPr3 , N2H4 and pyridine afforded mononuclear [Fe(L)('pyS4')] complexes with L = PMe3 ( 1 ), PnPr3 (2 ), N2H4 (3) and pyridine (4). NMR spectroscopy, magnetic measurements and X-ray structure determinations revealed that all complexes exhibit frans-thiolate donors and low-spin FeII centres, irrespective of the σ-π or σ ligand character of L. In this regard, the properties of [Fe(L)('pyS4')] complexes strongly contrast with those of [Fe(L)('NHS4')] complexes ('NHS4'2- = 2 ,2 '-bis(2 -mercaptophenylthio)- diethylamine(2 -)) and indicate that the rigid py(CH2)2 entity of the 'pyS42- ligand is able to enforce trans configurations and low-spin states of complexes with [FeNS4 ] cores. In spite of their diamagnetism, confirming the absence of antibonding electrons, all complexes 1 to 4 are highly reactive and rapidly exchange their L ligands for CO to give [Fe(CO)('pyS4')]. Evidence was obtained that the oxidation of [Fe(N'-H4)('pyS4')] (3) yields the diazene complex [μ-N2 H2 {Fe('pyS4’)}2] (5).
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Koval, Peter, Marc Barbry, and Daniel Sánchez-Portal. "PySCF-NAO: An efficient and flexible implementation of linear response time-dependent density functional theory with numerical atomic orbitals." Computer Physics Communications 236 (March 2019): 188–204. http://dx.doi.org/10.1016/j.cpc.2018.08.004.

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33

Brewster, N. K., D. L. Val, M. E. Walker, and J. C. Wallace. "Regulation of Pyruvate Carboxylase Isozyme (PYC1, PYC2) Gene Expression in Saccharomyces cerevisiae during Fermentative and Nonfermentative Growth." Archives of Biochemistry and Biophysics 311, no. 1 (May 1994): 62–71. http://dx.doi.org/10.1006/abbi.1994.1209.

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Menéndez, J. "Regulatory regions in the promoters of the Saccharomyces cerevisiae PYC1 and PYC2 genes encoding isoenzymes of pyruvate carboxylase." FEMS Microbiology Letters 164, no. 2 (July 15, 1998): 344–52. http://dx.doi.org/10.1016/s0378-1097(98)00237-7.

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35

Chauhan, Neeraj, Diane Inglis, Elvira Roman, Jesus Pla, Dongmei Li, Jose A. Calera, and Richard Calderone. "Candida albicans Response Regulator Gene SSK1 Regulates a Subset of Genes Whose Functions Are Associated with Cell Wall Biosynthesis and Adaptation to Oxidative Stress." Eukaryotic Cell 2, no. 5 (October 2003): 1018–24. http://dx.doi.org/10.1128/ec.2.5.1018-1024.2003.

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ABSTRACT Ssk1p of Candida albicans is a putative response regulator protein of the Hog1 two-component signal transduction system. In Saccharomyces cerevisiae, the phosphorylation state of Ssk1p determines whether genes that promote the adaptation of cells to osmotic stress are activated. We have previously shown that C. albicans SSK1 does not complement the ssk1 mutant of S. cerevisiae and that the ssk1 mutant of C. albicans is not sensitive to sorbitol. In this study, we show that the C. albicans ssk1 mutant is sensitive to several oxidants, including hydrogen peroxide, t-butyl hydroperoxide, menadione, and potassium superoxide when each is incorporated in yeast extract-peptone-dextrose (YPD) agar medium. We used DNA microarrays to identify genes whose regulation is affected by the ssk1 mutation. RNA from mutant cells (strain CSSK21) grown in YPD medium for 3 h at 30°C was reverse transcribed and then compared with similarly prepared RNA from wild-type cells (CAF2). We observed seven genes from mutant cells that were consistently up regulated (three-fold or greater compared to CAF2). In S. cerevisiae, three (AHP1, HSP12, and PYC2) of the seven genes that were up regulated provide cells with an adaptation function in response to oxidative stress; another gene (GPH1) is regulated under stress conditions by Hog1p. Three other genes that are up regulated encode a cell surface protein (FLO1), a mannosyl transferase (MNN4-4), and a putative two-component histidine kinase (CHK1) that regulates cell wall biosynthesis in C. albicans. Of the down-regulated genes, ALS1 is a known cell adhesin in C. albicans. Verification of the microarray data was obtained by reverse transcription-PCR for HSP12, AHP1, CHK1, PYC2, GPH1, ALS1, MNN4-4, and FLO1. To further determine the function of Ssk1p in the Hog1p signal transduction pathway in C. albicans, we used Western blot analysis to measure phosphorylation of Hog1p in the ssk1 mutant of C. albicans when grown under either osmotic or oxidative stress. We observed that Hog1p was phosphorylated in the ssk1 mutant of C. albicans when grown in a hyperosmotic medium but was not phosphorylated in the ssk1 mutant when the latter was grown in the presence of hydrogen peroxide. These data indicate that C. albicans utilizes the Ssk1p response regulator protein to adapt cells to oxidative stress, while its role in the adaptation to osmotic stress is less certain. Further, SSK1 appears to have a regulatory function in some aspects of cell wall biosynthesis. Thus, the functions of C. albicans SSK1 differ from those of S. cerevisiae SSK1.
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Waege, Ingrid, Georg Schmid, Sybille Thumann, Michael Thomm, and Winfried Hausner. "Shuttle Vector-Based Transformation System for Pyrococcus furiosus." Applied and Environmental Microbiology 76, no. 10 (April 2, 2010): 3308–13. http://dx.doi.org/10.1128/aem.01951-09.

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ABSTRACT Pyrococcus furiosus is a model organism for analyses of molecular biology and biochemistry of archaea, but so far no useful genetic tools for this species have been described. We report here a genetic transformation system for P. furiosus based on the shuttle vector system pYS2 from Pyrococcus abyssi. In the redesigned vector, the pyrE gene from Sulfolobus was replaced as a selectable marker by the 3-hydroxy-3-methylglutaryl coenzyme A reductase gene (HMG-CoA) conferring resistance of transformants to the antibiotic simvastatin. Use of this modified plasmid resulted in the overexpression of the HMG-CoA reductase in P. furiosus, allowing the selection of strains by growth in the presence of simvastatin. The modified shuttle vector replicated in P. furio s us, but the copy number was only one to two per chromosome. This system was used for overexpression of His6-tagged subunit D of the RNA polymerase (RNAP) in Pyrococcus cells. Functional RNAP was purified from transformed cells in two steps by Ni-NTA and gel filtration chromatography. Our data provide evidence that expression of transformed genes can be controlled from a regulated gluconeogenetic promoter.
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Zelle, Rintze M., Erik de Hulster, Wouter A. van Winden, Pieter de Waard, Cor Dijkema, Aaron A. Winkler, Jan-Maarten A. Geertman, Johannes P. van Dijken, Jack T. Pronk, and Antonius J. A. van Maris. "Malic Acid Production by Saccharomyces cerevisiae: Engineering of Pyruvate Carboxylation, Oxaloacetate Reduction, and Malate Export." Applied and Environmental Microbiology 74, no. 9 (March 14, 2008): 2766–77. http://dx.doi.org/10.1128/aem.02591-07.

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ABSTRACT Malic acid is a potential biomass-derivable “building block” for chemical synthesis. Since wild-type Saccharomyces cerevisiae strains produce only low levels of malate, metabolic engineering is required to achieve efficient malate production with this yeast. A promising pathway for malate production from glucose proceeds via carboxylation of pyruvate, followed by reduction of oxaloacetate to malate. This redox- and ATP-neutral, CO2-fixing pathway has a theoretical maximum yield of 2 mol malate (mol glucose)−1. A previously engineered glucose-tolerant, C2-independent pyruvate decarboxylase-negative S. cerevisiae strain was used as the platform to evaluate the impact of individual and combined introduction of three genetic modifications: (i) overexpression of the native pyruvate carboxylase encoded by PYC2, (ii) high-level expression of an allele of the MDH3 gene, of which the encoded malate dehydrogenase was retargeted to the cytosol by deletion of the C-terminal peroxisomal targeting sequence, and (iii) functional expression of the Schizosaccharomyces pombe malate transporter gene SpMAE1. While single or double modifications improved malate production, the highest malate yields and titers were obtained with the simultaneous introduction of all three modifications. In glucose-grown batch cultures, the resulting engineered strain produced malate at titers of up to 59 g liter−1 at a malate yield of 0.42 mol (mol glucose)−1. Metabolic flux analysis showed that metabolite labeling patterns observed upon nuclear magnetic resonance analyses of cultures grown on 13C-labeled glucose were consistent with the envisaged nonoxidative, fermentative pathway for malate production. The engineered strains still produced substantial amounts of pyruvate, indicating that the pathway efficiency can be further improved.
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38

Pérez-Torrente, Jesús J., Miguel A. Casado, Miguel A. Ciriano, Fernando J. Lahoz, and Luis A. Oro. "Synthesis of Rhodium, Iridium, and Palladium Tetranuclear Complexes Directed by 2,6-Dimercaptopyridine. X-ray Crystal Structure of [Rh4(μ-PyS2)2(cod)4] (cod = 1,5-Cyclooctadiene)." Inorganic Chemistry 35, no. 7 (January 1996): 1782–91. http://dx.doi.org/10.1021/ic950849u.

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Casado, Miguel A., Jesús J. Pérez-Torrente, Miguel A. Ciriano, Fernando J. Lahoz, and Luis A. Oro. "Tetranuclear [Rh4(μ-PyS2)2(diolefin)4] Complexes as Building Blocks for New Inorganic Architectures: Synthesis of Coordination Polymers and Heteropolynuclear Complexes with Electrophilic d8and d10Metal Fragments." Inorganic Chemistry 43, no. 4 (February 2004): 1558–67. http://dx.doi.org/10.1021/ic034893i.

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A. Casado, Miguel, Jesús J. Pérez Torrente, Andrew J. Edwards, Luis A. Oro, Miguel A. Ciriano, and Fernando J. Lahoz. "Rhodium?tetranuclear complexes as building blocks for the construction of coordination polymers: chiroselectivity in the formation of [ClCuRh4(µ-PyS2)2(cod)4]n (H2PyS2= 2,6-dimercaptopyridine)." CrystEngComm 2, no. 23 (2000): 125–27. http://dx.doi.org/10.1039/b004750n.

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41

Sellmann, Dieter, Jürgen Utz, and Frank W. Heinemann. "Transition Metal Complexes with Sulfur Ligands. 136.1Enforced Trans Coordination of Thiolate Donors in Electron Rich Iron, Ruthenium, and Nickel [M(L)(pyN2H2S2)] and [M(L)(pyS4)] Complexes (L = CO, PPh3, DMSO) (pyN2H2S22-= 2,6-Bis(2-mercaptophenylamino)dimethylpyridine(2−); pyS42-= 2,6-Bis(2-mercaptophenylthio)dimethylpyridine(2−))." Inorganic Chemistry 38, no. 23 (November 1999): 5314–22. http://dx.doi.org/10.1021/ic990169h.

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42

Dennis, Kristine, Ken Liu, ViLinh Tran, Bill Liang, Young-Mi Go, and Dean Jones. "Phytochelatin Characterization in Commonly Consumed Plant Foods Using Mass Spectrometry-based Metabolomics (P18-122-19)." Current Developments in Nutrition 3, Supplement_1 (June 1, 2019). http://dx.doi.org/10.1093/cdn/nzz039.p18-122-19.

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Abstract Objectives Phytochelatins (PyCs) are a group of metal-binding compounds formed by plants which could impact bioavailability of essential and toxic metals in the human diet. Liquid chromatography mass spectrometry (LC-MS)-based metabolomics can characterize a diversity of compounds from complex matrices. The aim of this project was to determine chromatographic characteristics of PyCs and their mass spectral signatures using authentic standards and determine if two types of PyCs (PyC2-Gly and PyC3-Gly) can be detected in commonly consumed plant foods and validated using chromatographic characteristics and ion dissociation mass spectrometry (MS/MS). Methods We analyzed PyC2-Gly and PyC3-Gly standards using LC-MS (C18 column; 10 minute analysis, positive ionization mode) at 5 different concentrations with 3 sample preparation methods to determine the common adducts, retention times, and optimal sample preparation methods. The most common adducts were selected for MS/MS to determine the characteristic fragmentation patterns. Onion, carrot, tomato, and whole wheat flour samples were analyzed with LC-MS/MS using the optimized sample preparation method and determined chromatographic and ion dissociation characteristics from the PyC standards analysis. Results For both PyC standards, the most common adducts were identified as follows: M + H > M + Na > M +K > 2M + H > 2M + Na = 2M + K > M + 2H and metabolite features had a retention time of 42 seconds. The fragmentation patterns for PyC2-Gly and PyC3-Gly for the M + H adduct (m/z 538.1272 and m/z 770.1790) were determined by MS/MS. The results show that fragmentation of m/z 538.1272 yielded m/z 231.0433, 334.0525, and 409.0844, and m/z 770.1790 yielded m/z 231.0433, 308.0911, and 641.1358. PyC2-Gly was detected in all 4 food types with a fragmentation pattern matching the PyC2-Gly standard while PyC3-Gly was not detected in these food samples. Conclusions This project defines chromatographic and mass spectrometry characteristics of PyC2-Gly and PyC3-Gly. Using these characteristics, we were able to validate PyC2-Gly in 4 plant foods. As PyCs may impact metal bioavailability from the diet, optimizing LC-MS detection methods for PyCs in plant foods facilitates future characterization of PyCs in the human diet. Funding Sources National Institute of Environmental Health Sciences.
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43

Heselpoth, Ryan D., Chad W. Euler, Raymond Schuch, and Vincent A. Fischetti. "Lysocins: Bioengineered Antimicrobials That Deliver Lysins across the Outer Membrane of Gram-Negative Bacteria." Antimicrobial Agents and Chemotherapy 63, no. 6 (April 8, 2019). http://dx.doi.org/10.1128/aac.00342-19.

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ABSTRACTThe prevalence of multidrug-resistantPseudomonas aeruginosahas stimulated development of alternative therapeutics. Bacteriophage peptidoglycan hydrolases, termed lysins, represent an emerging antimicrobial option for targeting Gram-positive bacteria. However, lysins against Gram-negatives are generally deterred by the outer membrane and their inability to work in serum. One solution involves exploiting evolved delivery systems used by colicin-like bacteriocins (e.g., S-type pyocins ofP. aeruginosa) to translocate through the outer membrane. Following surface receptor binding, colicin-like bacteriocins form Tol- or TonB-dependent translocons to actively import bactericidal domains through outer membrane protein channels. With this understanding, we developed lysocins, which are bioengineeredlysin-bacteriocinfusion molecules capable of periplasmic import. In our proof-of-concept studies, components from theP. aeruginosabacteriocin pyocin S2 (PyS2) responsible for surface receptor binding and outer membrane translocation were fused to the GN4 lysin to generate the PyS2-GN4 lysocin. PyS2-GN4 delivered the GN4 lysin to the periplasm to induce peptidoglycan cleavage and log-fold killing ofP. aeruginosawith minimal endotoxin release. While displaying narrow-spectrum antipseudomonal activity in human serum, PyS2-GN4 also efficiently disrupted biofilms, outperformed standard-of-care antibiotics, exhibited no cytotoxicity toward eukaryotic cells, and protected mice fromP. aeruginosachallenge in a bacteremia model. In addition to targetingP. aeruginosa, lysocins can be constructed to target other prominent Gram-negative bacterial pathogens.
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44

Xiberras, Joeline, Mathias Klein, Celina Prosch, Zahabiya Malubhoy, and Elke Nevoigt. "Anaplerotic reactions active during growth of Saccharomyces cerevisiae on glycerol." FEMS Yeast Research 20, no. 1 (December 10, 2019). http://dx.doi.org/10.1093/femsyr/foz086.

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ABSTRACT Anaplerotic reactions replenish TCA cycle intermediates during growth. In Saccharomyces cerevisiae, pyruvate carboxylase and the glyoxylate cycle have been experimentally identified to be the main anaplerotic routes during growth on glucose (C6) and ethanol (C2), respectively. The current study investigates the importance of the two isoenzymes of pyruvate carboxylase (PYC1 and PYC2) and one of the key enzymes of the glyoxylate cycle (ICL1) for growth on glycerol (C3) as a sole carbon source. As the wild-type strains of the CEN.PK family are unable to grow in pure synthetic glycerol medium, a reverse engineered derivative showing a maximum specific growth rate of 0.14 h−1 was used as the reference strain. While the deletion of PYC1 reduced the maximum specific growth rate by about 38%, the deletion of PYC2 had no significant impact, neither in the reference strain nor in the pyc1Δ mutant. The deletion of ICL1 only marginally reduced growth of the reference strain but further decreased the growth rate of the pyc1 deletion strain by 20%. Interestingly, the triple deletion (pyc1Δ pyc2Δ icl1Δ) did not show any growth. Therefore, both the pyruvate carboxylase and the glyoxylate cycle are involved in anaplerosis during growth on glycerol.
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Kocaaga, Ayca, Gunes Cakmak Genc, Sevim Karakas Celık, Rafet Koca, and Ahmet Dursun. "Association of NOD1, NOD2, PYDC1 and PYDC2 genes with Behcet’s disease susceptibility and clinical manifestations." Ophthalmic Genetics, July 23, 2021, 1–7. http://dx.doi.org/10.1080/13816810.2021.1955273.

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46

Orlev, L. "Does the dual-specificity MAPK phosphatase Pyst2-L lead a monogamous relationship with the Rek2 protein?" Immunology Letters, January 14, 2004. http://dx.doi.org/10.1016/j.mlet.2003.11.024.

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47

Gupta, Sanjeev K., Ankit Sharma, Hiralal Kushwaha, and Pratyoosh Shukla. "Over-expression of a Codon Optimized Yeast Cytosolic Pyruvate Carboxylase (PYC2) in CHO Cells for an Augmented Lactate Metabolism." Frontiers in Pharmacology 8 (July 17, 2017). http://dx.doi.org/10.3389/fphar.2017.00463.

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48

"Poster 216 acute intrathecal baclofen withdrawal due to a pharmaceutical compounding error: A case report. Maurice R. Bernaiche, DO (Michigan State Univ Coll Osteopathic Med, East Lansing, MI); Michael T. Andary, MD; Joel Bez, DO; J.J. Pysch, DO; Scott Kuhnert, MD, e-mail: bernaich@msu.edu." Archives of Physical Medicine and Rehabilitation 85, no. 9 (September 2004): e45. http://dx.doi.org/10.1016/j.apmr.2004.07.283.

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