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Journal articles on the topic 'Quantitative immunoblotting'

Author: Grafiati

Published: 4 June 2021

Last updated: 1 February 2022

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1

Váradi, Katalin, Jutta Schreiner, Barbara Plaimauer, et al. "ADAMTS13 autoantibody detection by quantitative immunoblotting." Blood 102, no. 5 (2003): 1932–33. http://dx.doi.org/10.1182/blood-2003-05-1688.

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2

Takekida, Kaori, Yutaka Kikuchi, Takeshi Yamazaki, et al. "Quantitative Analysis of Prion Protein by Immunoblotting." JOURNAL OF HEALTH SCIENCE 48, no. 3 (2002): 288–91. http://dx.doi.org/10.1248/jhs.48.288.

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3

Fernández, Emilio, and John J. Kopchick. "Quantitative determination of growth hormone by immunoblotting." Analytical Biochemistry 191, no. 2 (1990): 268–71. http://dx.doi.org/10.1016/0003-2697(90)90218-x.

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4

Janes, Kevin A. "An analysis of critical factors for quantitative immunoblotting." Science Signaling 8, no. 371 (2015): rs2. http://dx.doi.org/10.1126/scisignal.2005966.

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5

CHALMERS, RONALD M., and CHARLES A. FEWSON. "Quantitative immunoblotting in the study of bacterial evolution." Biochemical Society Transactions 16, no. 2 (1988): 153–54. http://dx.doi.org/10.1042/bst0160153.

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6

Schwarz, H. Peter, Mary Jo Heeb, Bernhard Lämmle, Mauro Berrettini, and John H. Griffin. "Quantitative Immunoblotting of Plasma and Platelet Protein S." Thrombosis and Haemostasis 56, no. 03 (1986): 382–86. http://dx.doi.org/10.1055/s-0038-1661680.

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SummaryProtein S, the activated protein C cofactor, was measured in plasma and in platelets by a quantitative immunoblotting assay using a double antibody technique. Either whole plasma or platelet lysates were electrophoresed on sodium dodecyl sulfate polyacrylamide gels and transferred to nitrocellulose membranes by electroblotting. Based on the demonstration of proportional transfer of protein S from the gel to the nitrocellulose membrane, a reproducible and sensitive quantitative assay for protein S antigen (∼ 70,000 MW) was developed that correlated well with the Laurell rocket assay for

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7

Lämmle, Bernhard, Mauro Berrettini, Hans Peter Schwarz, Mary Jo Heeb, and John B. Griffin. "Quantitative immunoblotting assay of blood coagulation factor XII." Thrombosis Research 41, no. 6 (1986): 747–59. http://dx.doi.org/10.1016/0049-3848(86)90373-7.

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8

Stahl, Dorothea, Sébastien Lacroix-Desmazes, Luc Mouthon, Srini V. Kaveri, and Michel D. Kazatchkine. "Analysis of human self-reactive antibody repertoires by quantitative immunoblotting." Journal of Immunological Methods 240, no. 1-2 (2000): 1–14. http://dx.doi.org/10.1016/s0022-1759(00)00185-x.

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9

Newman, Andrew J., Dennis Selkoe та Ulf Dettmer. "A New Method for Quantitative Immunoblotting of Endogenous α-Synuclein". PLoS ONE 8, № 11 (2013): e81314. http://dx.doi.org/10.1371/journal.pone.0081314.

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10

Schiavini, D. G., J. M. Puel, S. A. Averous, and J. A. Bazex. "Quantitative western immunoblotting analysis in survey of human immunodeficiency virus-seropositive patients." Journal of Clinical Microbiology 27, no. 9 (1989): 2062–66. http://dx.doi.org/10.1128/jcm.27.9.2062-2066.1989.

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11

Burckhardt, Christoph J., John D. Minna, and Gaudenz Danuser. "Co-immunoprecipitation and semi-quantitative immunoblotting for the analysis of protein-protein interactions." STAR Protocols 2, no. 3 (2021): 100644. http://dx.doi.org/10.1016/j.xpro.2021.100644.

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12

Zhu, Guangjing, Zhi Liu, Jonathan I. Epstein, et al. "A Novel Quantitative Multiplex Tissue Immunoblotting for Biomarkers Predicts a Prostate Cancer Aggressive Phenotype." Cancer Epidemiology Biomarkers & Prevention 24, no. 12 (2015): 1864–72. http://dx.doi.org/10.1158/1055-9965.epi-15-0496.

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13

Gettys, T. W., K. Sheriffcarter, F. J. Moomaw, I. L. Taylor та J. R. Raymond. "Characterization and Use of Crude α-Subunit Preparations for Quantitative Immunoblotting of G Proteins". Analytical Biochemistry 220, № 1 (1994): 82–91. http://dx.doi.org/10.1006/abio.1994.1302.

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14

Fredericks, Salim, Katie Bainbridge, Joanne F. Murray, Paul O. Collinson, Nicholas D. Carter, and David W. Holt. "Measurement of cardiac troponin I in striated muscle using three experimental methods." Annals of Clinical Biochemistry: International Journal of Laboratory Medicine 40, no. 3 (2003): 244–48. http://dx.doi.org/10.1258/000456303321610547.

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Background: Qualitative and quantitative measures of cardiac troponin I (cTnI) in striated muscle have been reported as part of diverse investigations. However, there is disparity in the literature regarding the findings of these analyses. The cTnI molecule can exist in phosphorylated, non-phosphorylated, reduced, non-reduced, complexed or non-complexed forms. Each of these forms can change the antigenicity of cTnI, resulting in different antibody-antigen interactions in different experimental formats, thereby giving rise to the disparities in the literature. Methods: cTnI in heart and skeleta

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15

Duez, H., C. Pelletier, S. Cools, et al. "A colony immunoblotting method for quantitative detection of a Bifidobacterium animalis probiotic strain in human faeces." Journal of Applied Microbiology 88, no. 6 (2000): 1019–27. http://dx.doi.org/10.1046/j.1365-2672.2000.01073.x.

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16

Chiou, Yee-Hsuan, Chung-Yee Yuo, Lin-Yu Wang, and Shiao-ping Huang. "Detection of Cross-Reactivity for Atopic Immunoglobulin E against Multiple Allergens." Clinical Diagnostic Laboratory Immunology 10, no. 2 (2003): 229–32. http://dx.doi.org/10.1128/cdli.10.2.229-232.2003.

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ABSTRACT The existence of specific immunoglobulin E (IgE) allows us to determine the allergens that cause the allergic disease. For the purposes of allergen avoidance and immunotherapy, the measurement of specific IgE is widely applied in clinical laboratories. However, if IgE from the serum of an allergic patient exhibits reactivity to multiple allergens, it would cause a problem. The present study analyzes whether the serum IgE with multiple reactivity is due to the presence of unique IgE against the common epitope shared by different allergens or the presence of multiple IgEs against differ

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17

Fulcher, CA, S. de Graaf Mahoney, and TS Zimmerman. "FVIII inhibitor IgG subclass and FVIII polypeptide specificity determined by immunoblotting." Blood 69, no. 5 (1987): 1475–80. http://dx.doi.org/10.1182/blood.v69.5.1475.1475.

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Abstract We used immunoblotting of purified factor VIII coagulant protein (FVIII) to localize FVIII inhibitor epitopes in 76 inhibitor plasmas to either the 92-kd FVIII polypeptide (and its 54-kd and/or 44-kd thrombin fragments), the 80-kd polypeptide (and its 72-kd thrombin fragment), or both of these polypeptides. We also used immunoblotting to examine the immunoglobulin class and subclass content of 12 inhibitors with monoclonal antibodies specific for human IgG subclasses and IgM. Seven hemophilic (alloantibody) and five spontaneous (autoantibody) inhibitors contained IgG-1 and IgG-4 antib

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18

Fulcher, CA, S. de Graaf Mahoney, and TS Zimmerman. "FVIII inhibitor IgG subclass and FVIII polypeptide specificity determined by immunoblotting." Blood 69, no. 5 (1987): 1475–80. http://dx.doi.org/10.1182/blood.v69.5.1475.bloodjournal6951475.

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We used immunoblotting of purified factor VIII coagulant protein (FVIII) to localize FVIII inhibitor epitopes in 76 inhibitor plasmas to either the 92-kd FVIII polypeptide (and its 54-kd and/or 44-kd thrombin fragments), the 80-kd polypeptide (and its 72-kd thrombin fragment), or both of these polypeptides. We also used immunoblotting to examine the immunoglobulin class and subclass content of 12 inhibitors with monoclonal antibodies specific for human IgG subclasses and IgM. Seven hemophilic (alloantibody) and five spontaneous (autoantibody) inhibitors contained IgG-1 and IgG-4 antibody; one

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19

Weghofer, M., W. R. Thomas, G. Pittner, F. Horak, R. Valenta, and S. Vrtala. "Comparison of purified Dermatophagoides pteronyssinus allergens and extract by two-dimensional immunoblotting and quantitative immunoglobulin E inhibitions." Clinical Experimental Allergy 35, no. 10 (2005): 1384–91. http://dx.doi.org/10.1111/j.1365-2222.2005.02345.x.

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20

Zhao, Fuguang, Bo Ma, Zhenye Lv, et al. "Zerumbone decreases BACH1 levels by upregulating miR- 708 to inhibit breast cancer cell proliferation and invasion." Tropical Journal of Pharmaceutical Research 19, no. 7 (2020): 1411–16. http://dx.doi.org/10.4314/tjpr.v19i7.11.

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Purpose: To investigate the potential mechanism by which zerumbone suppresses breast cancer (BC) cells.Methods: Cell viability and Transwell assays were performed to assess the effect of zerumbone on BC cell growth. The downstream target of zerumbone was determined using quantitative polymerase chain reaction assays and immunoblotting. Cell viability assays and immunoblotting were conducted to detect if zerumbone had any effect on BACH1 (BTB domain and CNC homolog 1) expression.Results: Zerumbone suppressed the proliferation, migration, and invasion of BC cells. It also upregulated the express

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21

Desoubeaux, Guillaume, Roman Peschke, Carolina Le-Bert, et al. "Seroprevalence Survey for Microsporidia in Common Bottlenose Dolphin (Tursiops truncatus): Example of a Quantitative Approach Based on Immunoblotting." Journal of Wildlife Diseases 54, no. 4 (2018): 870–73. http://dx.doi.org/10.7589/2017-11-287.

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22

Hisanaga, Shin-ichi. "Quantitative analysis of in vivo phosphorylation of Cyclin-dependent kinase activator p35 by Phos-tag SDS-PAGE/immunoblotting." SEIBUTSU BUTSURI KAGAKU 56, Suppl_1 (2012): s9—s13. http://dx.doi.org/10.2198/sbk.56.s9.

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23

Beier, Konstantin, Alfred Völkl, and H. Dariush Fahimi. "The impact of aging on enzyme proteins of rat liver peroxisomes: quantitative analysis by immunoblotting and immunoelectron microscopy." Virchows Archiv B Cell Pathology Including Molecular Pathology 63, no. 1 (1993): 139–46. http://dx.doi.org/10.1007/bf02899254.

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24

CHAPMAN, M., E. THOMPSON, P. CANDLER, R. DALE, A. CHURCH, and G. GIOVANNONI. "Quantitative demonstration of intrathecal synthesis of high affinity immunoglobulin G in herpes simplex encephalitis using affinity-mediated immunoblotting." Journal of Neuroimmunology 185, no. 1-2 (2007): 130–35. http://dx.doi.org/10.1016/j.jneuroim.2007.01.002.

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25

Bhakdi, Sucharit, Dieter Jenne, and Ferdinand Hugo. "Electroimmunoassay-Immunoblotting (EIA-IB) for the utilization of monoclonal antibodies in quantitative immunoelectropheresis: the method and its applications." Journal of Immunological Methods 80, no. 1 (1985): 25–32. http://dx.doi.org/10.1016/0022-1759(85)90160-7.

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26

Cartwright, I. J., and J. A. Higgins. "Quantification of apolipoprotein B-48 and B-100 in rat liver endoplasmic reticulum and Golgi fractions." Biochemical Journal 285, no. 1 (1992): 153–59. http://dx.doi.org/10.1042/bj2850153.

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We have developed a method for measurement of apolipoprotein (apo) B-48 and apo B-100 in blood and subcellular fractions of rat liver based on SDS/PAGE followed by quantitative immunoblotting using 125I-Protein A. Standard curves were prepared in each assay using apo B prepared from total rat lipoproteins by extraction with tetramethylurea. Subcellular fractions (rough and smooth endoplasmic reticulum and Golgi fractions) were prepared from rat liver and separated into membrane and cisternal-content fractions. For quantification, membrane fractions were solubilized in Triton X-100, and the apo

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27

Yu, Ming-Jiun, Trairak Pisitkun, Guanghui Wang, et al. "Large-scale quantitative LC-MS/MS analysis of detergent-resistant membrane proteins from rat renal collecting duct." American Journal of Physiology-Cell Physiology 295, no. 3 (2008): C661—C678. http://dx.doi.org/10.1152/ajpcell.90650.2007.

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In the renal collecting duct, vasopressin controls transport of water and solutes via regulation of membrane transporters such as aquaporin-2 (AQP2) and the epithelial urea transporter UT-A. To discover proteins potentially involved in vasopressin action in rat kidney collecting ducts, we enriched membrane “raft” proteins by harvesting detergent-resistant membranes (DRMs) of the inner medullary collecting duct (IMCD) cells. Proteins were identified and quantified with LC-MS/MS. A total of 814 proteins were identified in the DRM fractions. Of these, 186, including several characteristic raft pr

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28

Hollenbeck, P. J. "The distribution, abundance and subcellular localization of kinesin." Journal of Cell Biology 108, no. 6 (1989): 2335–42. http://dx.doi.org/10.1083/jcb.108.6.2335.

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An antiserum which binds kinesin specifically on Western blots was used to determine the distribution and abundance of chicken kinesin by correlated immunoblotting and immunolocalization. Quantitative immunoblotting showed that the abundance of kinesin varied widely in different cell and tissue types, from 0.039% of total protein in epidermal fibroblasts to 0.309% in sympathetic neurons; of the types examined, only red blood cells lacked detectable kinesin. The molar ratio of tubulin/kinesin varied over a narrower range. To analyze the intracellular distribution of kinesin, cultured fibroblast

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29

Berrettini, M., B. Lammle, T. White, et al. "Detection of in vitro and in vivo cleavage of high molecular weight kininogen in human plasma by immunoblotting with monoclonal antibodies." Blood 68, no. 2 (1986): 455–62. http://dx.doi.org/10.1182/blood.v68.2.455.455.

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Abstract Purified human high-mol-wt kininogen (HMWK), the cofactor of the contact phase of blood coagulation, migrated as a single band (approximately 110,000 mol wt) in a continuous buffer sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), but appeared as two separated bands (approximately 120,000 and 105,000 mol wt) when analyzed in a discontinuous buffer SDS-PAGE system. After elution from SDS polyacrylamide gels, each of the two bands showed coagulant activity. Six murine monoclonal antibodies (Mabs) against HMWK were produced and purified. In immunoblotting studies, thr

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30

Berrettini, M., B. Lammle, T. White, et al. "Detection of in vitro and in vivo cleavage of high molecular weight kininogen in human plasma by immunoblotting with monoclonal antibodies." Blood 68, no. 2 (1986): 455–62. http://dx.doi.org/10.1182/blood.v68.2.455.bloodjournal682455.

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Purified human high-mol-wt kininogen (HMWK), the cofactor of the contact phase of blood coagulation, migrated as a single band (approximately 110,000 mol wt) in a continuous buffer sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), but appeared as two separated bands (approximately 120,000 and 105,000 mol wt) when analyzed in a discontinuous buffer SDS-PAGE system. After elution from SDS polyacrylamide gels, each of the two bands showed coagulant activity. Six murine monoclonal antibodies (Mabs) against HMWK were produced and purified. In immunoblotting studies, three Mabs b

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31

Scandella, D., M. Mattingly, and R. Prescott. "A recombinant factor VIII A2 domain polypeptide quantitatively neutralizes human inhibitor antibodies that bind to A2." Blood 82, no. 6 (1993): 1767–75. http://dx.doi.org/10.1182/blood.v82.6.1767.1767.

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Abstract Human antibodies that inactivate coagulation factor VIII (fVIII), known as inhibitors, have been shown by immunoblotting or immunoprecipitation assays to bind predominantly to epitopes within the A2 and/or C2 domains of the fVIII protein. Because these assays simply measure antibody binding, a soluble recombinant polypeptide containing the fVIII A2 domain was used to develop a quantitative inhibitor neutralization assay for antibodies that bound only to A2 by immunoblotting assay. Antibodies from six of eight inhibitor plasmas were fully neutralized by A2 (> or = 90%), whereas two

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32

Scandella, D., M. Mattingly, and R. Prescott. "A recombinant factor VIII A2 domain polypeptide quantitatively neutralizes human inhibitor antibodies that bind to A2." Blood 82, no. 6 (1993): 1767–75. http://dx.doi.org/10.1182/blood.v82.6.1767.bloodjournal8261767.

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Human antibodies that inactivate coagulation factor VIII (fVIII), known as inhibitors, have been shown by immunoblotting or immunoprecipitation assays to bind predominantly to epitopes within the A2 and/or C2 domains of the fVIII protein. Because these assays simply measure antibody binding, a soluble recombinant polypeptide containing the fVIII A2 domain was used to develop a quantitative inhibitor neutralization assay for antibodies that bound only to A2 by immunoblotting assay. Antibodies from six of eight inhibitor plasmas were fully neutralized by A2 (> or = 90%), whereas two were only

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33

FRANKLIN, Isobel K., Robert A. WINZ, and Michael J. HUBBARD. "Endoplasmic reticulum Ca2+-ATPase pump is up-regulated in calcium-transporting dental enamel cells: a non-housekeeping role for SERCA2b." Biochemical Journal 358, no. 1 (2001): 217–24. http://dx.doi.org/10.1042/bj3580217.

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Dental enamel-forming cells face a major challenge to avoid the cytotoxic effects of excess calcium. We have characterized sarcoplasmic/endoplasmic reticulum calcium-ATPase pumps (SERCA) in rat enamel cells to address the proposal that non-mitochondrial calcium stores play a dominant role in transcellular calcium transport. A single major isoform, SERCA2b, was detected during the protein-secretory and calcium-transport stages of enamel formation using reverse-transcriptase PCR, cDNA cloning, Northern analysis and immunoblotting. Most importantly, SERCA2b exhibited a specific 3-fold up-regulati

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34

Tanaka, K., K. Omori, and Y. Tashiro. "Quantitative immunoferritin localization of leucine aminopeptidase on canine hepatocyte cell surface." Journal of Histochemistry & Cytochemistry 34, no. 6 (1986): 775–84. http://dx.doi.org/10.1177/34.6.3517150.

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Rabbit monospecific antibody against canine kidney leucine aminopeptidase (LAP) (EC.3.4.11.2) specifically immunoprecipitated kidney and also liver LAP activity from corresponding plasma membrane preparations previously solubilized with Triton X-100. Immunological specificity of the antibody was also shown by immunoblotting of LAP from canine and rat liver plasma membranes and by electrophoretic analyses of the precursor forms in MDCK cells. Canine liver was pre-fixed by perfusion with 0.6% glutaraldehyde and the dissociated liver cells were prepared without losing their polarized structure (2

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35

Ferro, A., C. Plumpton, and M. J. Brown. "Is Receptor Cross-Regulation in Human Heart Caused by Alterations in Cardiac Guanine Nucleotide-Binding Proteins?" Clinical Science 85, no. 4 (1993): 393–99. http://dx.doi.org/10.1042/cs0850393.

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1. Guanine nucleotide-binding proteins (G-proteins) play a central role in signal transduction between a wide variety of cell-surface receptors and intracellular second messenger systems. Recently, we and others have demonstrated that cross-regulation can occur between a variety of G-protein-linked receptors in human heart. Chronic β1-adrenoceptor blockade gives rise to sensitization of β2-adrenoceptor and of 5HT4-receptor responses, both of which are mediated via stimulation of adenylate cyclase through stimulatory G-proteins (Gs), and also gives rise to desensit-ization of muscarinic M2-rece

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36

Ji, Qiling, Xuemei Wang, Jianxin Cai, Xiangnan Du, Hui Sun та Nan Zhang. "MiR-22-3p Regulates Amyloid β Deposit in Mice Model of Alzheimer's Disease by Targeting Mitogen-activated Protein Kinase 14". Current Neurovascular Research 16, № 5 (2020): 473–80. http://dx.doi.org/10.2174/1567202616666191111124516.

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Propose: To investigate whether miR-22-3p is able to regulate AD development and its molecular mechanism. Methods: Morris water maze test was performed to test the spatial memory. Quantitative polymerase chain reaction (qPCR) was used to assess the expression level of miR-22-3p. The enzymelinked immunosorbent assay (ELISA) was used to assess the levels of Aβ40 and Aβ42. Immunoblotting analysis was performed to detect the protein expression levels of amyloid precursor protein (APP), mitogen-activated protein kinase 14 (MAPK14) and beta-site Amyloid precursor protein Cleaving Enzyme 1 (BACE1). L

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37

Song, Dongqiang, Beili Xu, Dongmin Shi, Shuyu Li, and Yu Cai. "S100A6 promotes proliferation and migration of HepG2 cells via increased ubiquitin-dependent degradation of p53." Open Medicine 15, no. 1 (2020): 317–26. http://dx.doi.org/10.1515/med-2020-0101.

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AbstractPurposeS100A6 protein (calcyclin), a small calcium-binding protein of the S100 family, is often upregulated in various types of cancers, including hepatocellular carcinoma (HCC). The aim of this study was to illustrate the molecular mechanism of S100A6 in regulating the proliferation and migration of HCC cells.MethodsThe expressions of S100A6 in human HCC and adjacent non-tumor liver specimens were detected using immunoblotting and quantitative PCR (qPCR). The recombinant glutathione S-transferase (GST)-tagged human S100A6 protein was purified and identified. After treatment with S100A

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38

Silva, Richard C., Beatriz A. Castilho, and Evelyn Sattlegger. "A Rapid Extraction Method for mammalian cell cultures, suitable for quantitative immunoblotting analysis of proteins, including phosphorylated GCN2 and eIF2a." MethodsX 5 (2018): 75–82. http://dx.doi.org/10.1016/j.mex.2017.10.008.

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39

Ehrhardt, R. A., and A. W. Bell. "Developmental increases in glucose transporter concentration in the sheep placenta." American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 273, no. 3 (1997): R1132—R1141. http://dx.doi.org/10.1152/ajpregu.1997.273.3.r1132.

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To explore the molecular basis for the gestational increase in glucose transport capacity of the sheep placenta in vivo, placentas from twin-pregnant ewes at days 75, 110, and 140 postcoitus (n = 6/group) were analyzed for glucose transporter (GT) concentration. Concentration (pmol/mg protein) of D-glucose-inhibitable binding sites, measured by [3H]cytochalasin B binding analysis, increased 3.4 times from mid- to late pregnancy. Concurrently, abundance of GLUT-1 and GLUT-3 protein, measured by immunoblotting with specific polyclonal antibodies, increased 2.3 and 2.9 times, respectively, while

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40

Zerlauth, G., J. Wesierska-Gadek, and G. Sauermann. "Fibronectin observed in the nuclear matrix of HeLa tumour cells." Journal of Cell Science 89, no. 3 (1988): 415–21. http://dx.doi.org/10.1242/jcs.89.3.415.

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We have investigated the intracellular distribution of insoluble fibronectin in HeLa tumour cells. By indirect immunofluorescence microscopy fibronectin was detected in the nuclear region, but not in the region of the cell surface. Isolated nuclei and isolated nuclear matrices also stained for fibronectin. By quantitative enzyme-linked immunosorbent assay (ELISA), fibronectin was found almost exclusively in the subcellular fraction of isolated nuclear matrices. Using immunoblotting techniques fibronectin was detected in nuclear matrices isolated from cells grown in standard and in fibronectin-

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41

Weisner, Abbie M., Henrik Chart, Andrew Bush, Jane C. Davies, and Tyrone L. Pitt. "Detection of antibodies to Pseudomonas aeruginosa in serum and oral fluid from patients with cystic fibrosis." Journal of Medical Microbiology 56, no. 5 (2007): 670–74. http://dx.doi.org/10.1099/jmm.0.46833-0.

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Cystic fibrosis (CF) patients who are chronically infected with Pseudomonas aeruginosa make serum antibodies to bacterial surface LPS as well as other pseudomonas antigens. This study investigated the feasibility of using oral fluid samples for the detection of pseudomonas antibodies in CF patients and compared these results with corresponding serum antibodies. Most strains of P. aeruginosa produce two forms of LPS molecule, termed A-band (described as a common antigen) and B-band (O-serotype-specific antigen), apparently bound to a common core oligosaccharide moiety. A-band LPS was demonstrat

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42

Aldridge, Georgina M., David M. Podrebarac, William T. Greenough, and Ivan Jeanne Weiler. "The use of total protein stains as loading controls: An alternative to high-abundance single-protein controls in semi-quantitative immunoblotting." Journal of Neuroscience Methods 172, no. 2 (2008): 250–54. http://dx.doi.org/10.1016/j.jneumeth.2008.05.003.

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43

Lee, Jen-Chieh, Chien-Feng Li, Fu-Min Fang, et al. "Prognostic implication of MET overexpression in myxofibrosarcomas: an integrative array comparative genomic hybridization, real-time quantitative PCR, immunoblotting, and immunohistochemical analysis." Modern Pathology 23, no. 10 (2010): 1379–92. http://dx.doi.org/10.1038/modpathol.2010.128.

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44

Fredericks, Salim, Joanne F. Murray, Michael Bewick, et al. "Cardiac Troponin T and Creatine Kinase MB Are Not Increased in Exterior Oblique Muscle of Patients with Renal Failure." Clinical Chemistry 47, no. 6 (2001): 1023–30. http://dx.doi.org/10.1093/clinchem/47.6.1023.

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Abstract Background: Serum cardiac troponin T (cTnT) concentrations may be increased in patients with renal dysfunction without evidence of cardiac damage, as assessed by conventional methods. It has been suggested that these positive measurements result from the expression in skeletal muscle of fetal isoforms of cTnT, which are detected by the cTnT immunoassay. Methods: Skeletal muscle (exterior oblique) biopsies were taken from healthy living kidney donors (n = 5) and transplant recipients (n = 19). The amounts of cTnT and creatine kinase (CK) isoenzymes in skeletal muscle of healthy control

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45

Ruediger, R., J. E. Van Wart Hood, M. Mumby, and G. Walter. "Constant expression and activity of protein phosphatase 2A in synchronized cells." Molecular and Cellular Biology 11, no. 8 (1991): 4282–85. http://dx.doi.org/10.1128/mcb.11.8.4282.

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The levels of the A, B, and C subunits of protein phosphatase 2A in extracts from synchronized embryonic bovine tracheal cells were determined by immunoblotting with subunit-specific antibodies. A constant amount of each subunit was found in resting cells as well as in growing cells from all stages of the cell cycle. The phosphatase activity of protein phosphatase 2A was also constant. A quantitative comparison showed that the A and C subunits were present in similar amounts, whereas the B subunit was present at a significantly lower level. Together, the A, B, and C subunits represented approx

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Ruediger, R., J. E. Van Wart Hood, M. Mumby, and G. Walter. "Constant expression and activity of protein phosphatase 2A in synchronized cells." Molecular and Cellular Biology 11, no. 8 (1991): 4282–85. http://dx.doi.org/10.1128/mcb.11.8.4282-4285.1991.

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The levels of the A, B, and C subunits of protein phosphatase 2A in extracts from synchronized embryonic bovine tracheal cells were determined by immunoblotting with subunit-specific antibodies. A constant amount of each subunit was found in resting cells as well as in growing cells from all stages of the cell cycle. The phosphatase activity of protein phosphatase 2A was also constant. A quantitative comparison showed that the A and C subunits were present in similar amounts, whereas the B subunit was present at a significantly lower level. Together, the A, B, and C subunits represented approx

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Raykha, Christina N., Justin D. Crawford, Andrea F. Burry та ін. "IGF2 expression and β-catenin levels are increased in Frozen Shoulder Syndrome". Clinical & Investigative Medicine 37, № 4 (2014): 262. http://dx.doi.org/10.25011/cim.v37i4.21733.

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Purpose: Frozen Shoulder Syndrome is a fibrosis of the shoulder joint capsule that is clinically associated with Dupuytren’s disease, a fibrosis of the palmar fascia. Little is known about any commonalities in the pathophysiology of these connective tissue fibroses. β-catenin, a protein that transactivates gene expression, and levels of IGF2 mRNA, encoding insulin-like growth factor-II, are elevated in Dupuytren’s disease. The aim of this study was to determine if correlating changes in β-catenin levels and IGF2 expression are evident in Frozen Shoulder Syndrome. Methods: Tissue from patients

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Chen, Yinan, Changhui Sun, Jiong Lu, et al. "MicroRNA-590-5p antagonizes the inhibitory effect of high glucose on osteoblast differentiation by suppressing Smad7 in MC3T3-E1 cells." Journal of International Medical Research 47, no. 4 (2019): 1740–48. http://dx.doi.org/10.1177/0300060519830212.

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Objective MicroRNA-590-5p (miR-590-5p) has been reported to stimulate osteoblast differentiation; however, its effect in diabetic osteoporosis remains unknown. This study investigated the effect of miR-590-5p on high glucose (HG)-suppressed osteoblast differentiation. Methods The effect of HG on MC3T3-E1 cell survival was assessed using the MTT assay. The expression levels and activities of osteoblastic proteins were evaluated by quantitative reverse transcription polymerase chain reaction (qRT-PCR), alkaline phosphatase (ALP) assay, and immunoblotting assay. Tumor growth factor-β (TGF-β) sign

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Banan, A., J. Z. Fields, D. A. Talmage, Yang Zhang та A. Keshavarzian. "PKC-β1 mediates EGF protection of microtubules and barrier of intestinal monolayers against oxidants". American Journal of Physiology-Gastrointestinal and Liver Physiology 281, № 3 (2001): G833—G847. http://dx.doi.org/10.1152/ajpgi.2001.281.3.g833.

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Using monolayers of human intestinal (Caco-2) cells, we found that oxidants and ethanol damage the cytoskeleton and disrupt barrier integrity; epidermal growth factor (EGF) prevents damage by enhancement of protein kinase C (PKC) activity and translocation of the PKC-β1 isoform. To see if PKC-β1 mediates EGF protection, cells were transfected to stably over- or underexpress PKC-β1. Transfected monolayers were preincubated with low or high doses of EGF (1 or 10 ng/ml) or 1-oleoyl-2-acetyl- sn-glycerol [OAG; a PKC activator (0.01 or 50 μM)] before treatment with oxidant (0.5 mM H2O2). Only in mo

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Colin-Pierre, Charlie, Valérie Untereiner, Ganesh D. Sockalingum, et al. "Hair Histology and Glycosaminoglycans Distribution Probed by Infrared Spectral Imaging: Focus on Heparan Sulfate Proteoglycan and Glypican-1 during Hair Growth Cycle." Biomolecules 11, no. 2 (2021): 192. http://dx.doi.org/10.3390/biom11020192.

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The expression of glypicans in different hair follicle (HF) compartments and their potential roles during hair shaft growth are still poorly understood. Heparan sulfate proteoglycan (HSPG) distribution in HFs is classically investigated by conventional histology, biochemical analysis, and immunohistochemistry. In this report, a novel approach is proposed to assess hair histology and HSPG distribution changes in HFs at different phases of the hair growth cycle using infrared spectral imaging (IRSI). The distribution of HSPGs in HFs was probed by IRSI using the absorption region relevant to sulf

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