Relevant bibliographies by topics / Quantitative real-time PCR TaqMan probe

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  2. Dissertations / Theses
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  4. Book chapters
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Academic literature on the topic 'Quantitative real-time PCR TaqMan probe'

Author: Grafiati
Published: 4 June 2021
Last updated: 5 February 2022

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Journal articles on the topic "Quantitative real-time PCR TaqMan probe"

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Lee, Jae Il, and In Seop Kim. "TaqMan Probe Real-Time PCR for Quantitative Detection of Mycoplasma during Manufacture of Biologics." KSBB Journal 29, no. 5 (2014): 361–71. http://dx.doi.org/10.7841/ksbbj.2014.29.5.361.

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Weller, S. A., J. G. Elphinstone, N. C. Smith, N. Boonham, and D. E. Stead. "Detection of Ralstonia solanacearumStrains with a Quantitative, Multiplex, Real-Time, Fluorogenic PCR (TaqMan) Assay." Applied and Environmental Microbiology 66, no. 7 (2000): 2853–58. http://dx.doi.org/10.1128/aem.66.7.2853-2858.2000.

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ABSTRACT A fluorogenic (TaqMan) PCR assay was developed to detectRalstonia solanacearum strains. Two fluorogenic probes were utilized in a multiplex reaction; one broad-range probe (RS) detected all biovars of R. solanacearum, and a second more specific probe (B2) detected only biovar 2A. Amplification of the target was measured by the 5′ nuclease activity of Taq DNA polymerase on each probe, resulting in emission of fluorescence. TaqMan PCR was performed with DNA extracted from 42 R. solanacearum and genetically or serologically related strains to demonstrate the specificity of the assay. In
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Jothikumar, Narayanan, Theresa L. Cromeans, Vincent R. Hill, Xiaoyan Lu, Mark D. Sobsey, and Dean D. Erdman. "Quantitative Real-Time PCR Assays for Detection of Human Adenoviruses and Identification of Serotypes 40 and 41." Applied and Environmental Microbiology 71, no. 6 (2005): 3131–36. http://dx.doi.org/10.1128/aem.71.6.3131-3136.2005.

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ABSTRACT A quantitative real-time TaqMan PCR assay for detection of human adenoviruses (HAdV) was developed using broadly reactive consensus primers and a TaqMan probe targeting a conserved region of the hexon gene. The TaqMan assay correctly identified 56 representative adenovirus prototype strains and field isolates from all six adenovirus species (A to F). Based on infectious units, the TaqMan assay was able to detect as few as 0.4 and 0.004 infectious units of adenovirus serotype 2 (AdV2) and AdV41, respectively, with results obtained in less than 90 min. Using genomic equivalents, the bro
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Liu, Ya-Mei, Liang Qiu, An-Zhi Sheng, Xiao-Yuan Wan, Dong-Yuan Cheng, and Jie Huang. "Quantitative detection method of Enterocytozoon hepatopenaei using TaqMan probe real-time PCR." Journal of Invertebrate Pathology 151 (January 2018): 191–96. http://dx.doi.org/10.1016/j.jip.2017.12.006.

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Rosenbergova, K., P. Lany, Z. Pospisil, O. Kubicek, V. Celer, and D. Molinkova. "Quantification of avian influenza virus in tissues of mute swans using TaqMan real time qRT-PCR." Veterinární Medicína 54, No. 9 (2009): 435–43. http://dx.doi.org/10.17221/16/2009-vetmed.

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This study reports on the first quantification of avian influenza virus in the organs of mute swans that died during the epizootic of avian influenza (H5N1) between January and April 2006 in the Czech Republic. The quantitative real-time Reverse Transcriptase PCR (qRT-PCR) assay based on a TaqMan probe was developed for a rapid detection and quantification of avian influenza virus RNA in clinical samples collected from mute swans. Conserved regions in the matrix protein gene of avian influenza virus served as targets for the primers and TaqMan probe design. A recombinant plasmid containing the
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Li, Jin, Lilin Wang, Pasi A. Jänne, and G. Mike Makrigiorgos. "Coamplification at Lower Denaturation Temperature-PCR Increases Mutation-Detection Selectivity of TaqMan-Based Real-Time PCR." Clinical Chemistry 55, no. 4 (2009): 748–56. http://dx.doi.org/10.1373/clinchem.2008.113381.

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Abstract Background: DNA genotyping with mutation-specific TaqMan® probes (Applied Biosystems) is broadly used in detection of single-nucleotide polymorphisms but is less so for somatic mutations because of its limited selectivity for low-level mutations. We recently described coamplification at lower denaturation temperature-PCR (COLD-PCR), a method that amplifies minority alleles selectively from mixtures of wild-type and mutation-containing sequences during the PCR. We demonstrate that combining COLD-PCR with TaqMan technology provides TaqMan genotyping with the selectivity needed to detect
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Jiang, Qianli, Shan Jiang, Fanyi Meng, et al. "Efficient Duplex Real-Time Quantitative RT-PCR of BCR-ABL and ABL Gene Transcripts for Monitoring of Minimal Residual Disease." Blood 112, no. 11 (2008): 4887. http://dx.doi.org/10.1182/blood.v112.11.4887.4887.

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Abstract BCL-ABL fusion gene can be found in 100% chronic myelogenous leukemia (CML) and about 25% adult acute lympholid leukemia where its expression level is a crucial parameter for monitoring of minimal residual disease (MRD). Depending on the breakpoint in BCR, exon 2 of ABL (a2) joins with exons 1 (e1), 13 (b2), or 14 (b3), or rarely to exon 19 (e19) of BCR resulting in chimeric proteins of p190, p210 and p230, respectively. The c-ABL gene is one of the best controls for MRD detection by real-time quantitative RT-PCR (RQ-RT-PCR). In most studies published before, PCR probes were labeled b
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Pusterla, Nicola, Jon B. Huder, Christian M. Leutenegger, Ueli Braun, John E. Madigan, and Hans Lutz. "Quantitative Real-Time PCR for Detection of Members of the Ehrlichia phagocytophila Genogroup in Host Animals and Ixodes ricinus Ticks." Journal of Clinical Microbiology 37, no. 5 (1999): 1329–31. http://dx.doi.org/10.1128/jcm.37.5.1329-1331.1999.

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A TaqMan PCR was established for identification and quantitation of members of the Ehrlichia phagocytophila group in experimentally infected cows and in Ixodes ricinus ticks. The TaqMan PCR identified a 106-bp section of the 16S rRNA gene by use of a specific fluorogenic probe and two primers. This technique was specific for members of the E. phagocytophila group, which include E. phagocytophila, Ehrlichia equi, and the agent of human granulocytic ehrlichiosis. The TaqMan system identified 10 copies of a cloned section of the 16S rRNA gene ofE. phagocytophila. The sensitivity and specificity o
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Boyer, S., and J. Yang. "Measurement of gene amplification in FFPE tumor tissues by quantitative real-time PCR in comparison with FISH." Journal of Clinical Oncology 25, no. 18_suppl (2007): 14086. http://dx.doi.org/10.1200/jco.2007.25.18_suppl.14086.

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14086 Background: Amplified oncogenes such as HER2 and EGFR have proven to be good targets for molecular therapy. Recent retrospective analyses suggest that EGFR amplification predicate better responses to EGFR kinase inhibitors. However, different methods were used to measure EGFR gene amplification and polysomy in these studies, including FISH and various real-time quantitative PCR platforms, which may have led to the different conclusions reported by these groups. In this study, we compared Taqman- QPCR with FISH in scoring EGFR and HER2 amplification and EGFR polysomy on 42 FFPE tumor samp
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Lin, Mei-Hui, Tse-Ching Chen, Tseng-tong Kuo, Ching-Chung Tseng, and Ching-Ping Tseng. "Real-Time PCR for Quantitative Detection ofToxoplasma gondii." Journal of Clinical Microbiology 38, no. 11 (2000): 4121–25. http://dx.doi.org/10.1128/jcm.38.11.4121-4125.2000.

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The protozoan Toxoplasma gondii is one of the most common infectious pathogenic parasites and can cause severe medical complications in infants and immunocompromised individuals. We report here the development of a real-time PCR-based assay for the detection of T. gondii. Oligonucleotide primers and a fluorescence-labeled TaqMan probe were designed to amplify the T. gondii B1 gene. After 40 PCR cycles, the cycle threshold values (CT) indicative of the quantity of the target gene were determined. Typically, a CT of 25.09 was obtained with DNA from 500 tachyzoites of the T. gondii RH strain. The
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Dissertations / Theses on the topic "Quantitative real-time PCR TaqMan probe"

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Steyn, HC, A. Pretorius, and CME McCrindle. "A quantitative real-time PCR assay for Ehrlichia ruminantium using pCS20." Elsevier, 2008. http://encore.tut.ac.za/iii/cpro/DigitalItemViewPage.external?sp=1000379.

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Heartwater is a tick borne disease that affects ruminants and wild animals in Africa south of the Sahara. It is caused by Ehrlichia ruminantium and transmitted by the tick Amblyomma hebraeum. The protocols currently used to detect heartwater take several days to complete. Here, we describe the development of a pCS20 quantitative real-time PCRTaqMan probe assay to detect E. ruminantium in livestock blood and ticks from the field. The assay is based on the conserved pCS20 gene region of E. ruminantium that contains two overlapping genes, rnc and ctaG [Collins, N.E., Liebenberg, J., De Villiers,
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Kessler, Yvonne. "Quantitative TaqMan® real-time PCR assays for gene expression normalisation in feline tissues /." [S.l.] : [s.n.], 2009. http://opac.nebis.ch/cgi-bin/showAbstract.pl?sys=000292626.

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Stupin, Jens [Verfasser]. "Quantitative mRNA-Bestimmung von Zytokinen aus mononukleären Nabelschnurblutzellen nach Geburten von Schwangeren mit Verdacht auf ein Amnioninfektionssyndrom mittels real-time TaqMan RT-PCR / Jens Stupin." Berlin : Medizinische Fakultät Charité - Universitätsmedizin Berlin, 2010. http://d-nb.info/1024865932/34.

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Setu, Dambhare. "Development of a specific and sensitive method for detection and quantification of Ustilago nuda by qPCR." Thesis, Högskolan i Skövde, Institutionen för biovetenskap, 2021. http://urn.kb.se/resolve?urn=urn:nbn:se:his:diva-20243.

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Loose smut of barley, caused by fungal pathogen Ustilago nuda is one of the major concerns throughout the globe for barley producers. The infection takes place without exhibiting any obvious symptoms and an infected seed lot can only be identified at the heading stage when the fungal teliospores emerge at the place of crop. The percentage losses on yield are directly proportional to the occurrence of infection. Currently available detection methods include seed health testing protocols which are time-consuming and cumbersome. With the globalization of the international market and increased cro
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Eulálio, Inês Mariana Cardoso. "Developmente of a CNVs detection method through qPCR." Master's thesis, Universidade de Aveiro, 2017. http://hdl.handle.net/10773/22507.

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Mestrado em Biotecnologia<br>Os copy number variations (CNVs) consistem em segmentos de DNA de uma kilobase ou mais, que se encontram num número variável de cópias, em comparação com um genoma de referência. A deteção de CNVs é convencionalmente realizada através de técnicas de citogenética, como fluorescence in situ hybridization e array comparative genomic hybridization, ou com base em PCR, como multiplex ligation-dependent probe amplification, SNP arrays ou deep sequencing. Porém, a evolução da técnica de PCR quantitativo em tempo real (qPCR) permitiu que fosse, actualmente, considerada o m
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Chen, Pei-Shih, and 陳培詩. "Real-time quantitative PCR with Gene Probe, Fluorochrome and Flow Cytometry for Bioaerosols." Thesis, 2005. http://ndltd.ncl.edu.tw/handle/62509276124378183270.

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博士<br>國立臺灣大學<br>環境衛生研究所<br>93<br>Traditional culture and microscopy methods for evaluation of bioaerosols are slow, tedious, and rather imprecise. Here, using pure suspensions of E. coli, three non-culture methods, namely, flow cytometry (FCM), epifluorescence microscopy (EFM), and real-time quantitative polymerase chain reaction (real-time qPCR), were compared with a traditional culture-based method. Then, the optimal conditions of FCM with 5 different fluorescent dyes (FCM/FL) were evaluated in laboratory samples and validated to field study. In addition, FCM/FL was applied to evaluate th
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Pei-Shih, Chen. "Environmental Bioaerosol Monitoring - Real-time quantitative PCR with Gene Probe, Fluorochrome and Flow Cytometry." 2005. http://www.cetd.com.tw/ec/thesisdetail.aspx?etdun=U0001-3101200509324400.

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Books on the topic "Quantitative real-time PCR TaqMan probe"

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Taberlet, Pierre, Aurélie Bonin, Lucie Zinger, and Eric Coissac. Single-species detection. Oxford University Press, 2018. http://dx.doi.org/10.1093/oso/9780198767220.003.0009.

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Chapter 9 “Single-species detection” deals with the practical aspects of detecting a single and predefined taxon with eDNA, with a particular focus on the use of quantitative PCR (qPCR) for this purpose. After presenting how single-species detection has been implemented in a few seminal studies, it details the principles underlying qPCR. More specifically, it describes the typical qPCR amplification curve and the different systems (SYBR green and TaqMan probe assays) available to record amplicon accumulation in real time via fluorescence measurements. Chapter 9 also explains how the initial nu
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Book chapters on the topic "Quantitative real-time PCR TaqMan probe"

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Vaisman, Boris L. "Genotyping of Transgenic Animals by Real-Time Quantitative PCR with TaqMan Probes." In Methods in Molecular Biology. Humana Press, 2013. http://dx.doi.org/10.1007/978-1-60327-369-5_11.

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Dötsch, Jörg, Ellen Schoof, and Wolfgang Rascher. "Quantitative TaqMan Real-Time PCR." In Medical Biomethods Handbook. Humana Press, 2005. http://dx.doi.org/10.1385/1-59259-870-6:305.

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Dötsch, Jörg, Ellen Schoof, and Wolfgang Rascher. "Quantitative TaqMan Real-Time PCR: Diagnostic and Scientific Applications." In Molecular Analysis and Genome Discovery. John Wiley & Sons, Ltd, 2005. http://dx.doi.org/10.1002/0470020202.ch2.

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Baric, Sanja. "Duplex TaqMan Real-Time PCR for Rapid Quantitative Analysis of a Phytoplasma in Its Host Plant without External Standard Curves." In Phytoplasmas. Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-8837-2_10.

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Conference papers on the topic "Quantitative real-time PCR TaqMan probe"

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Xu, Qiong, Wenjia Gu, Yinan Zhang, et al. "Quantitative Detection of raw sheep in Meat Products by a TaqMan Real-Time PCR Assay." In International Conference on Chemical,Material and Food Engineering. Atlantis Press, 2015. http://dx.doi.org/10.2991/cmfe-15.2015.10.

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Britto, Constança, Carolina Messias, Otacilio Moreira, et al. "Development of Quantitative Real-Time PCR (TaqMan Triplex System) for the Diagnosis and Evaluation of Therapeutic Efficacy in Chagas Disease." In IV International Symposium on Immunobiologicals & VII Seminário Anual Científico e Tecnológico. Instituto de Tecnologia em Imunobiológicos, 2019. http://dx.doi.org/10.35259/isi.sact.2019_32808.

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