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Journal articles on the topic 'Quartet de microtubules'

Author: Grafiati
Published: 4 June 2021
Last updated: 1 February 2022

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1

Gottlieb, R. A., and D. B. Murphy. "Analysis of the microtubule-binding domain of MAP-2." Journal of Cell Biology 101, no. 5 (1985): 1782–89. http://dx.doi.org/10.1083/jcb.101.5.1782.

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We examined the microtubule-binding domain of the microtubule-associated protein (MAP), MAP-2, using rabbit antibodies that specifically bind to the microtubule-binding region ("stub") and the projection portion ("arm") of MAP-2. We found that (a) microtubules decorated with arm antibody look similar to those labeled with whole unfractionated MAP antibody, though microtubules are not labeled with stub antibody; (b) incubation of depolymerized microtubule protein with stub antibody prior to assembly partially inhibits the rate of microtubule elongation, presumably because MAPs that are complexe
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2

Miller, Rita K., Soo-Chen Cheng, and Mark D. Rose. "Bim1p/Yeb1p Mediates the Kar9p-dependent Cortical Attachment of Cytoplasmic Microtubules." Molecular Biology of the Cell 11, no. 9 (2000): 2949–59. http://dx.doi.org/10.1091/mbc.11.9.2949.

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In Saccharomyces cerevisiae, positioning of the mitotic spindle depends on the interaction of cytoplasmic microtubules with the cell cortex. In this process, cortical Kar9p in the bud acts as a link between the actin and microtubule cytoskeletons. To identify Kar9p-interacting proteins, a two-hybrid screen was conducted with the use of full-length Kar9p as bait, and three genes were identified: BIM1, STU2, andKAR9 itself. STU2 encodes a component of the spindle pole body. Bim1p is the yeast homologue of the human microtubule-binding protein EB1, which is a binding partner to the adenomatous po
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3

Vaughn, Kevin C., and Larry P. Lehnen. "Mitotic Disrupter Herbicides." Weed Science 39, no. 3 (1991): 450–57. http://dx.doi.org/10.1017/s0043174500073215.

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Approximately one-quarter of all herbicides that have been marketed affect mitosis as a primary mechanism of action. All of these herbicides appear to interact directly or indirectly with the microtubule. Dinitroaniline and phosphoric amide herbicides inhibit microtubule polymerization from free tubulin subunits. Because of the loss of spindle and kinetochore microtubules, chromosomes cannot move to the poles during mitosis, resulting in cells exhibiting an arrested prometaphase configuration. Nuclear membranes re-form around the chromosomal masses to form lobed nuclei. Cortical microtubules,
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4

Loiodice, Isabelle, Marcel Janson, Penny Tavormina, et al. "Quantifying Tubulin Concentration and Microtubule Number Throughout the Fission Yeast Cell Cycle." Biomolecules 9, no. 3 (2019): 86. http://dx.doi.org/10.3390/biom9030086.

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The fission yeast Schizosaccharomyces pombe serves as a good genetic model organism for the molecular dissection of the microtubule (MT) cytoskeleton. However, analysis of the number and distribution of individual MTs throughout the cell cycle, particularly during mitosis, in living cells is still lacking, making quantitative modelling imprecise. We use quantitative fluorescent imaging and analysis to measure the changes in tubulin concentration and MT number and distribution throughout the cell cycle at a single MT resolution in living cells. In the wild-type cell, both mother and daughter sp
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5

Franzen, Rachelle, Sandra L. Tanner, Suzanne M. Dashiell, Catherine A. Rottkamp, Jeffrey A. Hammer, and Richard H. Quarles. "Microtubule-associated protein 1B." Journal of Cell Biology 155, no. 6 (2001): 893–98. http://dx.doi.org/10.1083/jcb.200108137.

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Myelin-associated glycoprotein (MAG) is expressed in periaxonal membranes of myelinating glia where it is believed to function in glia–axon interactions by binding to a component of the axolemma. Experiments involving Western blot overlay and coimmunoprecipitation demonstrated that MAG binds to a phosphorylated neuronal isoform of microtubule-associated protein 1B (MAP1B) expressed in dorsal root ganglion neurons (DRGNs) and axolemma-enriched fractions from myelinated axons of brain, but not to the isoform of MAP1B expressed by glial cells. The expression of some MAP1B as a neuronal plasma mem
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6

Stephens, R. E., S. Oleszko-Szuts, and R. W. Linck. "Retention of ciliary ninefold structure after removal of microtubules." Journal of Cell Science 92, no. 3 (1989): 391–402. http://dx.doi.org/10.1242/jcs.92.3.391.

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When axonemes of isolated gill cilia from the bay scallop Aequipecten irradians are heated at 45 degrees C for a minimum of 8 min in a 10 mM-Tris-HCl (pH 8), 1 mM-EDTA solution, nearly 80% of the tubulin is solubilized but most minor structural proteins are retained in a ninefold symmetrical configuration. This remnant consists of the junctional protofilaments, derived from outer doublet tubules, interconnected by nexin linkages, with radial spoke components still directed inwards. The remnant is of the same length as the original cilium, with the junctional protofilaments attached at the dist
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7

Esson, Heather J., Brooke Morriswood, Sevil Yavuz, Keni Vidilaseris, Gang Dong, and Graham Warren. "Morphology of the Trypanosome Bilobe, a Novel Cytoskeletal Structure." Eukaryotic Cell 11, no. 6 (2012): 761–72. http://dx.doi.org/10.1128/ec.05287-11.

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ABSTRACT The trypanosome bilobe is a cytoskeletal structure of unclear function. To date, four proteins have been shown to localize stably to it: TbMORN1, TbLRRP1, TbCentrin2, and TbCentrin4. In this study, a combination of immunofluorescence microscopy and electron microscopy was used to explore the morphology of the bilobe and its relationship to other nearby cytoskeletal structures in the African trypanosome procyclic trypomastigote. The use of detergent/salt-extracted flagellum preparations was found to be an effective way of discerning features of the cytoskeletal ultrastructure that are
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8

Zhang, Zhechun, Yonathan Goldtzvik, and D. Thirumalai. "Parsing the roles of neck-linker docking and tethered head diffusion in the stepping dynamics of kinesin." Proceedings of the National Academy of Sciences 114, no. 46 (2017): E9838—E9845. http://dx.doi.org/10.1073/pnas.1706014114.

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Kinesin walks processively on microtubules (MTs) in an asymmetric hand-over-hand manner consuming one ATP molecule per 16-nm step. The individual contributions due to docking of the approximately 13-residue neck linker to the leading head (deemed to be the power stroke) and diffusion of the trailing head (TH) that contributes in propelling the motor by 16 nm have not been quantified. We use molecular simulations by creating a coarse-grained model of the MT–kinesin complex, which reproduces the measured stall force as well as the force required to dislodge the motor head from the MT, to show th
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9

De Diego Otero, Yolanda, Lies-Anne Severijnen, Gert van Cappellen, Mariëtte Schrier, Ben Oostra, and Rob Willemsen. "Transport of Fragile X Mental Retardation Protein via Granules in Neurites of PC12 Cells." Molecular and Cellular Biology 22, no. 23 (2002): 8332–41. http://dx.doi.org/10.1128/mcb.22.23.8332-8341.2002.

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ABSTRACT Lack of fragile X mental retardation protein (FMRP) causes fragile X syndrome, a common form of inherited mental retardation. FMRP is an RNA binding protein thought to be involved in translation efficiency and/or trafficking of certain mRNAs. Recently, a subset of mRNAs to which FMRP binds with high affinity has been identified. These FMRP-associated mRNAs contain an intramolecular G-quartet structure. In neurons, dendritic mRNAs are involved in local synthesis of proteins in response to synaptic activity, and this represents a mechanism for synaptic plasticity. To determine the role
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10

Darling, Sarah, Andrew B. Fielding, Dorota Sabat-Pośpiech, Ian A. Prior, and Judy M. Coulson. "Regulation of the cell cycle and centrosome biology by deubiquitylases." Biochemical Society Transactions 45, no. 5 (2017): 1125–36. http://dx.doi.org/10.1042/bst20170087.

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Post-translational modification of proteins by ubiquitylation is increasingly recognised as a highly complex code that contributes to the regulation of diverse cellular processes. In humans, a family of almost 100 deubiquitylase enzymes (DUBs) are assigned to six subfamilies and many of these DUBs can remove ubiquitin from proteins to reverse signals. Roles for individual DUBs have been delineated within specific cellular processes, including many that are dysregulated in diseases, particularly cancer. As potentially druggable enzymes, disease-associated DUBs are of increasing interest as phar
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11

Iwamoto, Mitsuhiko, Nayuko Sato, Risa Terasawa, et al. "Phase II study of eribulin mesylate as first- or second-line therapy for metastatic HER2-negative breast cancer." Journal of Clinical Oncology 31, no. 15_suppl (2013): TPS1140. http://dx.doi.org/10.1200/jco.2013.31.15_suppl.tps1140.

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TPS1140 Background: Eribulin mesylate is a nontaxane microtubule dynamics inhibitor that is approved in patients with metastatic breast cancer who have previously received at least two chemotherapeutic regimens (including an anthracycline and a taxane) for metastatic breast cancer. In a previous phase III study, eribulin mesylate demonstrated to significant improvement in overall survival in patients with heavily pretreated, locally recurrent or metastatic breast cancer when compared to treatment of physician’s choice. The majority of patients were HER2-negative and all had previously failed 2
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12

Wang, Xiaolong, Yongliang Fan, Yifan He, et al. "Integrative Analysis of miRNA and mRNA Expression Profiles in Mammary Glands of Holstein Cows Artificially Infected with Staphylococcus aureus." Pathogens 10, no. 5 (2021): 506. http://dx.doi.org/10.3390/pathogens10050506.

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Staphylococcus aureus- induced mastitis is one of the most intractable problems for the dairy industry, which causes loss of milk yield and early slaughter of cows worldwide. Few studies have used a comprehensive approach based on the integrative analysis of miRNA and mRNA expression profiles to explore molecular mechanism in bovine mastitis caused by S. aureus. In this study, S. aureus (A1, B1 and C1) and sterile phosphate buffered saline (PBS) (A2, B2 and C2) were introduced to different udder quarters of three individual cows, and transcriptome sequencing and microarrays were utilized to de
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13

Park, S. W., Y. W. Jeong, J. J. Kim, et al. "73 IMPROVEMENT OF CANINE CLONING EFFICIENCY BY OPTIMIZED DONOR CELL PREPARATION." Reproduction, Fertility and Development 22, no. 1 (2010): 195. http://dx.doi.org/10.1071/rdv22n1ab73.

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The Tibetan Mastiff is the oldest dog breed in the world, and it is at the edge of extinction. Li et al. (2008) believe that protection of and research on the Tibetan Mastiff is extremely urgent, yet few studies have been carried out, particularly at the molecular level. Somatic cell nuclear transfer (SCNT) is an efficient technique for the conservation of endangered animals because it can increase the number of individuals within a population. Considering the virtually unlimited value of cloned canids in critical biotechnology applications, including gene conservation of endangered canids and
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14

Dong, Xiaoduo, Teck Kwang Lim, Qingsong Lin, and Cynthia Y. He. "Basal Body Protein TbSAF1 Is Required for Microtubule Quartet Anchorage to the Basal Bodies in Trypanosoma brucei." mBio 11, no. 3 (2020). http://dx.doi.org/10.1128/mbio.00668-20.

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ABSTRACT Sperm flagellar protein 1 (Spef1, also known as CLAMP) is a microtubule-associated protein involved in various microtubule-related functions from ciliary motility to polarized cell movement and planar cell polarity. In Trypanosoma brucei, the causative agent of trypanosomiasis, a single Spef1 ortholog (TbSpef1) is associated with a microtubule quartet (MtQ), which is in close association with several single-copied organelles and is required for their coordinated biogenesis during the cell cycle. Here, we investigated the interaction network of TbSpef1 using BioID, a proximity-dependen
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15

Chadourne, Manon, Elodie Poumerol, Luc Jouneau, et al. "Structural and Functional Characterization of a Testicular Long Non-coding RNA (4930463O16Rik) Identified in the Meiotic Arrest of the Mouse Topaz1–/– Testes." Frontiers in Cell and Developmental Biology 9 (July 1, 2021). http://dx.doi.org/10.3389/fcell.2021.700290.

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Spermatogenesis involves coordinated processes, including meiosis, to produce functional gametes. We previously reported Topaz1 as a germ cell-specific gene highly conserved in vertebrates. Topaz1 knockout males are sterile with testes that lack haploid germ cells because of meiotic arrest after prophase I. To better characterize Topaz1–/– testes, we used RNA-sequencing analyses at two different developmental stages (P16 and P18). The absence of TOPAZ1 disturbed the expression of genes involved in microtubule and/or cilium mobility, biological processes required for spermatogenesis. Moreover,
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