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1
Anand, Shashi, Mohammad Aslam Khan, Moh’d Khushman, Santanu Dasgupta, Seema Singh, and Ajay Pratap Singh. "Comprehensive Analysis of Expression, Clinicopathological Association and Potential Prognostic Significance of RABs in Pancreatic Cancer." International Journal of Molecular Sciences 21, no. 15 (2020): 5580. http://dx.doi.org/10.3390/ijms21155580.
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RAB proteins (RABs) represent the largest subfamily of Ras-like small GTPases that regulate a wide variety of endosomal membrane transport pathways. Their aberrant expression has been demonstrated in various malignancies and implicated in pathogenesis. Using The Cancer Genome Atlas (TCGA) database, we analyzed the differential expression and clinicopathological association of RAB genes in pancreatic ductal adenocarcinoma (PDAC). Of the 62 RAB genes analyzed, five (RAB3A, RAB26, RAB25, RAB21, and RAB22A) exhibited statistically significant upregulation, while five (RAB6B, RAB8B, RABL2A, RABL2B, and RAB32) were downregulated in PDAC as compared to the normal pancreas. Racially disparate expression was also reported for RAB3A, RAB25, and RAB26. However, no clear trend of altered expression was observed with increasing stage and grade, age, and gender of the patients. PDAC from occasional drinkers had significantly higher expression of RAB21 compared to daily or weekly drinkers, whereas RAB25 expression was significantly higher in social drinkers, compared to occasional ones. The expression of RABL2A was significantly reduced in PDAC from diabetic patients, whereas RAB26 was significantly lower in pancreatitis patients. More importantly, a significant association of high expression of RAB21, RAB22A, and RAB25, and low expression of RAB6B, RABL2A, and RABL2B was observed with poorer survival of PC patients. Together, our study suggests potential diagnostic and prognostic significance of RABs in PDAC, warranting further investigations to define their functional and mechanistic significance.
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Tasaka, K., N. Masumoto, J. Mizuki, et al. "Rab3B is essential for GnRH-induced gonadotrophin release from anterior pituitary cells." Journal of Endocrinology 157, no. 2 (1998): 267–74. http://dx.doi.org/10.1677/joe.0.1570267.
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Gonadotrophin-releasing hormone (GnRH) induces the release of gonadotrophins via an increase in cytosolic Ca2+ concentration ([Ca2+]). Rab3B, a member of the small GTP-binding protein Rab family, is known to be involved in Ca(2+)-regulated exocytosis in pituitary cells. However, it is not known whether Rab3B functions in the physiological process regulated by GnRH in gonadotrophs. In this study using antisense oligonucleotide against Rab3B (AS-Rab3B) we determined that Rab3B is involved in GnRH-induced gonadotrophin release. Rab3B immunopositive cells were reduced in 24% of pituitary cells by AS-Rab3B. This treatment did not affect the population of gonadotrophs or the intracellular contents of gonadotrophins. However, AS-Rab3B significantly inhibited the total amount of basal and GnRH-induced gonadotrophin released from pituitary cells. These results show that Rab3B is involved in basal and GnRH-induced gonadotrophins release but not the storage of gonadotrophins. Next, the changes in [Ca2+] and exocytosis in gonadotrophs treated with AS-Rab3B were compared among Rab3B-positive and -negative cells. The change in [Ca2+] was not different in the two groups, but exocytosis was significantly inhibited in Rab3B-negative cells. These results suggest that Rab3B is essential for GnRH-regulated exocytosis downstream of cytosolic Ca2+ in gonadotrophs.
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Wong, Wing Hei, Stephanie Z. Liu, Annie Shi Ru Li, Xingyou Liu, Morris F. Manolson, and Ralph A. Zirngibl. "Evidence for Rab7b and Its Splice Isoforms Having Distinct Biological Functions from Rab7a." International Journal of Molecular Sciences 26, no. 6 (2025): 2610. https://doi.org/10.3390/ijms26062610.
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The Rab family of small guanosine triphosphatases (GTPases) are nucleotide-dependent switches. Mutations in Rabs can result in human diseases. Rab7a and Rab7b transition from early endosomes to lysosomes and are presumed to function similarly. Most studies look at Rab7a, less on Rab7b, with the underlying assumption they function similarly. There have yet to be articles comparing them side by side. Whilst cloning Rab7 homologues, we identified splice isoforms for Rab7b only. These splice isoforms, Rab7b2 and Rab7bx8 lacking different exons, have not been previously characterized but suggest alternative function(s) for Rab7b. Thus, we hypothesize that Rab7 homologues have distinct functions. Here, we compare Rab7a and Rab7b nucleotide mutants locked in GDP-bound (Rab7T22N), GTP-bound (Rab7Q67L), nucleotide-free (Rab7aN125I/Rab7bN124I) states and characterized localization of the Rab7b splice isoforms. HeLa cells were transiently transfected with fluorescently tagged Rab7 reporters. Confocal images were processed with ImageJ and analyzed with SPSS. Rab7a and Rab7b nucleotide mutants were significantly different to one another. Approximately 50% of Rab7b splice isoform-expressing cells had aggregated vesicles, which were phenotypically different from Rab7b vesicles. Rab7a and Rab7b vesicles shared approximately 60% colocalization with each other, while Rab7b vesicles preferentially localized to the Trans Golgi Network. Our results suggest Rab7b is distinct from Rab7a, and Rab7b splice isoforms have different biological functions.
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Aulia Pratama Ricardo and Wimbrayardi Wimbrayardi. "Deskriptif Proses Pembuatan Alat Musik Rabab Pasisia di Painan Timur Kabupaten Pesisir Selatan." Imajinasi : Jurnal Ilmu Pengetahuan, Seni, dan Teknologi 1, no. 2 (2024): 10–23. http://dx.doi.org/10.62383/imajinasi.v1i2.127.
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The process of making traditional musical instruments Rabab Pasisia in South Pesisir Regency. This study aims to describe the process of making Rabab Pasisia musical instruments. The approach used is a qualitative approach with a descriptive type of research. This research was conducted with data collection techniques used are observation, interview and documentation techniques. Data analysis techniques are carried out by reducing, presenting data and verifying this data aims to find out the structure, process, manufacturing techniques, playing techniques, from Rabab Pasisia. This musical instrument is made of jariang wood and has different tones, and uses several types of wood. The results of this study show that the parts and stages of the process of making traditional Rabab Pasisia musical instruments include: wooden mangkapatiah for Rabab bodies, sketching rabab patterns, making rabab tongues, installing rabab friction nylon, making rabab kudo-kudo, and how to produce sounds, tuning systems on traditional Rabab Pasisia musical instruments. The instruments used in the process of making traditional musical instruments Gandang Tambua include: Lantiak Knife (containing rabab tongue), Kapatiah (forming rabab body), Cutting Saw, Hammer (Stick), Measuring instrument (meter), Pencil, Sandpaper, Scissors. The ingredients are; Jariang Wood (45Cm Long, 30Cm Wide, and 4Cm Thick), Montiah Wood (Meranti), Sicerek Wood, Shell, Rope, Nails and, Wood glue. The process of making Rabab Pasisia musical instruments includes several stages, namely: 1) The process of making the body (jariang wood), until the finishing of fine and rough sanding, 2) The process of installing nylon on the rabab frictioner, 3) The process of installing squirrels, kudo-kudo, talingo and rabab strings for tuning the tone.
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Parwati, Silpa, and Harisnal Hadi. "PENGARUH PERTUNJUKAN ORGEN TUNGGAL TERHADAP EKSISTENSI RABAB PASISIA DI KEC. LENGAYANG KABUPATEN PESISIR SELATAN." Jurnal Sendratasik 9, no. 4 (2020): 45. http://dx.doi.org/10.24036/jsu.v9i1.109539.
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This study aims to find and describe the effect of Orgen Tunggal performance on the existence of Rabab Pasisiain Kambang Village, Lengayang District, South Pesisir Regency. This is a qualitative research using a descriptivemethod. The main instrument in this study was the researcher it self and was assisted by supporting instruments such as writing instruments and cameras. The data were collected through literature study, observation, interview, and documentation. The steps for analyzing the data are collecting the data, describing the data, and making conclusions. The results show that Orgen Tunggal performance can affect the existence of Rabab Pasisia in Kambang Village community, Lengayang District, South Pesisir Regency. Nowadays, people no longer understand the messages contained in Rabab, and Rababis viewed as entertainment only. There are factors which influence the existence of RababPasisia. Internally, the community more likely enjoys Orgen Tunggal. The people no longer understand the education value in Rabab, so it makes Rabab Pasisia unattractive. Externally, modernization and globalization rises. The existence of Orgen Tunggal which is increasingly popular in the community of KambangVillage, Lengayang District, affects the existence of Rabab as traditional entertainment which has moral messages.Gradually it also affects the intensity of Rabab performance on the community who enjoys Rabab, on messages Rabab delivers, on the existence of Rabab, and on the performance of Rabab. Rabab undergoes a few updates so that it still exists and is favored by the community. One of which is a form of Raun Sabalik which has pleasant rhythms.Keywords: influence, single orgen, the existence of rabab pasisia
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Meng, Tong. "CSIG-35. RAB3B DICTATES MTORC1/S6 SIGNALING IN CHORDOMA AND PREDICTS RESPONSE TO MTORC1-TARGETED THERAPY." Neuro-Oncology 26, Supplement_8 (2024): viii71. http://dx.doi.org/10.1093/neuonc/noae165.0284.
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Abstract Chordoma is a rare mesenchymal malignancy, exhibiting a high tendency to postoperative recurrence and poor prognosis. To date, its tumorigenic regulatory mechanisms remain elusive leading to a lack of effective therapeutic targets and drug sensitivity indicators. Here, via transcriptome and proteome analyses, we unveiled RAB3B as the prominent oncogenic regulator in chordoma. Multidimensional tissue samples further indicated its overexpression and enhancer-associated high transcriptional activity in chordoma. Notably, RAB3B ablation attenuated the chordoma cell growth, stemness, migration and tumorigenicity in vivo and in vitro. Through determining the RAB3B-mediated program in chordoma, we identified that RAB3B was highly associated with PI3K-AKT-mTOR signaling and directly bound to S6. Mechanistically, RAB3B increased the S6K-mediated phosphorylation of S6 by physically interacting with dephosphorylase DUSP12, to block its dephosphorylating of p-S6 specifically at S235/236. Pharmacological targeting mTORC1 pathway via dactolisib and rapamycin dramatically impeded the RAB3B-induced chordoma cell oncogenesis, while RAB3B knockout desensitized mTORC1 inhibition. Clinically, RAB3B/p-S6 suggested a good prognostic value and response to mTORC1 inhibitors for chordoma patients. Altogether, this work uncovers RAB3B as a novel activator of mTORC1 pathway, and suggests the oncogenic and predictive roles of RAB3B/p-S6 in chordoma, indicating therapeutic potentials of mTORC1-targeted therapy in advanced chordoma patients with aberrant RAB3B/p-S6 hyperactivation.
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Zhang, Yiwen, Zhen Yang, Ke Dai, et al. "Rab4b Promotes Cytolethal Distending Toxin from Glaesserella parasuis-Induced Cytotoxicity in PK-15 Cells." Toxins 16, no. 9 (2024): 407. http://dx.doi.org/10.3390/toxins16090407.
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Glaesserella parasuis cytolethal distending toxin (GpCDT) can induce cell cycle arrest and apoptosis. Our laboratory’s previous work demonstrated that GTPase 4b (Rab4b) is a key host protein implicated in GpCDT-induced cytotoxicity. This study investigated the probable involvement of Rab4b in the process. Our study used CRISPR/Cas9 technology to create a Rab4b-knockout cell line. The results showed greater resistance to GpCDT-induced cell cytotoxicity. In contrast, forced Rab4b overexpression increased GpCDT-induced cytotoxicity. Further immunoprecipitation study reveals that GpCDT may bind with Rab4b. In PK-15 cells, GpCDT is transported to the early endosomes and late endosomes, while after knocking out Rab4b, GpCDT cannot be transported to the early endosome via vesicles. Rab4b appears essential for GpCDT-induced cytotoxicity in PK-15 cells.
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Monetta, Pablo, Ileana Slavin, Nahuel Romero, and Cecilia Alvarez. "Rab1b Interacts with GBF1 and Modulates both ARF1 Dynamics and COPI Association." Molecular Biology of the Cell 18, no. 7 (2007): 2400–2410. http://dx.doi.org/10.1091/mbc.e06-11-1005.
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Assembly of the cytosolic coat protein I (COPI) complex at the ER–Golgi interface is directed by the ADP ribosylation factor1 (Arf1) and its guanine nucleotide exchange factor (GBF1). Rab1b GTPase modulates COPI recruitment, but the molecular mechanism underlying this action remains unclear. Our data reveal that in vivo expression of the GTP-restricted Rab1b mutant (Rab1Q67L) increased the association of GBF1 and COPI to peripheral structures localized at the ER exit sites (ERES) interface. Active Rab1b also stabilized Arf1 on Golgi membranes. Furthermore, we characterized GBF1 as a new Rab1b effector, and showed that its N-terminal domain was involved in this interaction. Rab1b small interfering RNA oligonucleotide assays suggested that Rab1b was required for GBF1 membrane association. To further understand how Rab1b functions in ER-to-Golgi transport, we analyzed GFP-Rab1b dynamics in HeLa cells. Time-lapse microscopy indicated that the majority of the Rab1b-labeled punctuated structures are relatively short-lived with limited-range movements. FRAP of Golgi GFP-Rab1bwt showed rapid recovery (t1/2 120 s) with minimal dependence on microtubules. Our data support a model where Rab1b-GTP induces GBF1 recruitment at the ERES interface and at the Golgi complex where it is required for COPII/COPI exchange or COPI vesicle formation, respectively.
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Maiben alpandi and Zulkarnain Mistortoify. "TUKANG RABAB DAN KABA RABAB PASISIA DALAM ACARA BARALEK DI NAGARI TALAO." Sorai: Jurnal Pengkajian dan Penciptaan Musik 17, no. 1 (2024): 45–54. http://dx.doi.org/10.33153/sorai.v17i1.6074.
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The study "Tukang Rabab dan Kaba Rabab Pasisia dalam Acara Baralek di Nagari Talao, Sumatra Barat" explores the competencies and presentation of kaba rabab pasisia in baralek events. This performance reveals the richness of Minangkabau culture, from a warm opening to a meaningful core story. Through poetic lyrics and captivating music, Kaba enriches understanding of Minangkabau cultural values. Tukang Rabab, as cultural guardians, adeptly perform rabab pasisia and master Minangkabau language and literature. Social interaction is key to their competence, living within the community of rabab pasisia artists and exchanging knowledge and skills. Despite facing modernization challenges, the art of rabab pasisia remains relevant and valuable. The participation of tukang rabab in cultural events, art training, and preservation projects strengthens Minangkabau identity and cultural heritage. This performance fosters community solidarity and serves as a platform for learning life values, enriching and preserving Minangkabau cultural identity, and encouraging the younger generation to uphold ancestral traditions
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Zhao, Qin, Chang Miao, Yi-Ting Chen, et al. "Host Factor Rab4b Promotes Japanese Encephalitis Virus Replication." Microorganisms 12, no. 9 (2024): 1804. http://dx.doi.org/10.3390/microorganisms12091804.
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Although the Japanese encephalitis virus (JEV) infects various cell types, its receptor molecules are still not clearly understood. In our laboratory’s prior research, Rab4b was identified as a potential host factor that facilitates JEV infection in PK15 cells, utilizing a genome-wide CRISPR/Cas9 knockout library (PK-15-GeCKO). To further explore the effect of Rab4b on JEV replication, we used the Rab4b knockout PK15 cell line using the CRISPR/Cas9 technology and overexpressing the Rab4b PK15 cell line, with IFA, RT–qPCR, and Western blot to study the effect of Rab4b on viral replication in the whole life cycle of the JEV. The results show that the knockout of Rab4b inhibited the replication of the JEV in PK15 cells, and the overexpression of Rab4b promoted the replication of the JEV in PK15 cell lines. Furthermore, we demonstrated for the first time that host factor Rab4b facilitates the adsorption, internalization, assembly, and release of the JEV, thereby promoting JEV replication. This study enriches the regulatory network between the JEV and host factors and lays the experimental foundation for further understanding of the function of the Rab4b protein.
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Kottorou, Anastasia, Foteinos-Ioannis Dimitrakopoulos, Georgia Diamantopoulou, et al. "Small Extracellular Vesicles (sEVs) Biogenesis Molecular Players Are Associated with Clinical Outcome of Colorectal Cancer Patients." Cancers 15, no. 6 (2023): 1685. http://dx.doi.org/10.3390/cancers15061685.
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A growing number of studies have shed light on the role of small extracellular vesicles (sEVs), including exosomes, in colorectal cancer (CRC). Available data regarding the clinical significance of molecular players in CRC, implicated in sEVs biogenesis, is limited. In this study, we assessed the expression of the most important genes which are implicated in sEVs biogenesis and their association with sEVs plasma levels, investigated with a double sandwich ELISA assay, as well as with the clinical outcome of patients with CRC. Our study shows that RAB27A, RAB27B, RAB2B, and RAB3B mRNA levels were lower in tumor tissues compared to tumor adjacent, non-malignant tissues (p < 0.001, p = 0.009, p = 0.011, and p < 0.001, respectively). In addition, high tumor expression of RAB27A, RAB27B, RAB9A, RAB11B, and STX1A was favorable of a 5-year survival (p = 0.038, p = 0.015, p = 0.008, p = 0.002, and p = 0.028, respectively). Furthermore, patients with adenomas had lower overall plasma sEVs concentrations, compared to healthy volunteers (p = 0.026), while no statistically significant differences were observed in the overall or tumor-derived plasma sEVs concentration (p = 0.885 and p = 0.330, respectively) of CRC patients. In conclusion, sEVs biogenesis has a potentially significant role in CRC, with RAB27A, RAB27B, RAB9A, RAB11B, and STX1A having a promising role in survival outcomes.
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Romero, Nahuel, Catherine I. Dumur, Hernán Martinez, et al. "Rab1b overexpression modifies Golgi size and gene expression in HeLa cells and modulates the thyrotrophin response in thyroid cells in culture." Molecular Biology of the Cell 24, no. 5 (2013): 617–32. http://dx.doi.org/10.1091/mbc.e12-07-0530.
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Rab1b belongs to the Rab-GTPase family that regulates membrane trafficking and signal transduction systems able to control diverse cellular activities, including gene expression. Rab1b is essential for endoplasmic reticulum–Golgi transport. Although it is ubiquitously expressed, its mRNA levels vary among different tissues. This work aims to characterize the role of the high Rab1b levels detected in some secretory tissues. We report that, in HeLa cells, an increase in Rab1b levels induces changes in Golgi size and gene expression. Significantly, analyses applied to selected genes, KDELR3, GM130 (involved in membrane transport), and the proto-oncogene JUN, indicate that the Rab1b increase acts as a molecular switch to control the expression of these genes at the transcriptional level, resulting in changes at the protein level. These Rab1b-dependent changes require the activity of p38 mitogen-activated protein kinase and the cAMP-responsive element-binding protein consensus binding site in those target promoter regions. Moreover, our results reveal that, in a secretory thyroid cell line (FRTL5), Rab1b expression increases in response to thyroid-stimulating hormone (TSH). Additionally, changes in Rab1b expression in FRTL5 cells modify the specific TSH response. Our results show, for the first time, that changes in Rab1b levels modulate gene transcription and strongly suggest that a Rab1b increase is required to elicit a secretory response.
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Opdam, F. J., A. Echard, H. J. Croes, et al. "The small GTPase Rab6B, a novel Rab6 subfamily member, is cell-type specifically expressed and localised to the Golgi apparatus." Journal of Cell Science 113, no. 15 (2000): 2725–35. http://dx.doi.org/10.1242/jcs.113.15.2725.
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Members of the Rab subfamily of small GTPases play an important role in the regulation of intracellular transport routes. Rab6A has been shown to be a regulator of membrane traffic from the Golgi apparatus towards the endoplasmic reticulum (ER). Here, we report on the identification of a Rab6 isoform, termed Rab6B. The corresponding full-length cDNA was isolated from a Caco-2 cell library. The deduced amino acid sequence showed 91% identity with the Rab6A protein and revealed that sequence divergence is dispersed over a large region of the COOH-terminal domain. Rab6B is encoded by an independent gene which is located on chromosome 3 region q21-q23. In contrast to Rab6A whose expression is ubiquitous, northern blot analysis, immunohistochemistry, and immunofluorescence demonstrated that Rab6B is expressed in a tissue and cell-type specific manner. Rab6B is predominantly expressed in brain and the neuroblastoma cell line SK-N-SH. In brain, Rab6B was found to be specifically expressed in microglia, pericytes and Purkinje cells. Endogenous Rab6B localises to the Golgi apparatus and to ERGIC-53-positive vesicles. Comparable studies between Rab6A and Rab6B revealed distinct biochemical and cellular properties. Rab6B displayed lower GTP-binding activities and in overexpression studies, the protein is distributed over Golgi and ER membranes, whereas Rab6A is more restricted to the Golgi apparatus. Since the GTP-bound form of Rab6B (Rab6B Q72L) does interact with all known Rab6A effectors, including Rabkinesin-6, the results suggest a cell-type specific role for Rab6B in retrograde membrane traffic at the level of the Golgi complex.
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He, Donghua, Taoyong Chen, Mingjin Yang, et al. "Rab7b Promotes Megakaryocytic Differentiation of K562 Cells by Activating the Protein Kinase C/Extracellular Signal-Regulated Kinase Dependent Pathway." Blood 114, no. 22 (2009): 3612. http://dx.doi.org/10.1182/blood.v114.22.3612.3612.
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Abstract Abstract 3612 Poster Board III-548 Rab proteins are small GTPases belonging to the Ras superfamily and involved in cell protein transportation processes such as internalization and exocytosis. Recently it has been found that several Rab proteins such as Rab7 are also essential for signaling and the controlling of cell proliferation and differentiation. We previously have identified a small GTPase homologous to Rab7, named Rab7b, which is localized to lysosome-associated compartments and is selectively expressed in promyeloid or monocytic cells. We demonstrated that rab7b is involved in monocytic differentiation of human acute promyelocytic leukemia cells. In this study, we detected the expression level of Rab7b during the process of PMA induced megakaryocytic differentiation of K562 cells, transfected Rab7b and its mutants into K562 cells, and investigated the function and signaling mechanism of PMA induced megakaryocytic differentiation of K562 cells. Firstly, we treated K562 cells with PMA for various times, detected the expression level of Rab7b by quantitative PCR and Western blot, and measured the expression level of CD11b, CD14, CD33 and CD41a on the cell surface. Next, we constructed Rab7b, Rab7b-Q67L (active GTPase-deficient mutant), Rab7b-ΔCC (localization deficiency) and Rab7b-RNAi expression vectors, which were then transfected into K562 cells. Megakaryocytic differentiation of cells were analyzed after treated with PMA for various times, including cell morphology and adhesive properties, cell growth arrest, polyploidization, secretion of IL-6 and expression of specific marker CD41a. Because of the considerable role of PKC and ERK1/2 signaling pathways in the megakaryocytic differentiation of K562 cells, we treated various K562 cells with PMA for various times and detected the activation of ERK1/2 by Western blot. Later, cells were inhibited by PKC and ERK1/2 specific inhibitors and treated with PMA for various times, and then the expression of Rab7b and megakaryocytic differentiation of K562 cells were observed. At last, PMA-induced activation of PKC and ERK1/2 signaling has been suggested to regulate cell cycle arrest and megakaryocyte differentiation by modulating the expression of the p21waf/cip1 and the dephosphorylation of the Rb protein, which were then detected by us after transfection of Rab7b and inhibition of PKC/MAPK pathways in K562 cells. The expression of Rab7b was upregulated during the PMA-induced megakaryocytic differentiation of K562 cells. Overexpression of wild-type and active mutant (Q67L) of Rab7b enhanced PMA stimulated megakaryocytic differentiation and ERK1/2 activation. In addition, overexpression of the localization deficient mutant (ΔCC) or RNAi of Rab7b inhibited PMA-stimulated megakaryocytic differentiation and ERK1/2 activation. PMA-induced megakaryocytic differentiation and activation of ERK1/2 in K562 cells was reversed by inhibiting either PKC or ERK1/2 signaling pathway, whereas the mRNA and protein expression of Rab7b was only suppressed in PKC inhibiting groups, indicating that Rab7b mediates a signal for megakaryocytic differentiation downstream of PKC and upstream of ERK1/2. Therefore, we concluded that Rab7b could promote PMA induced differentiation of K562 cells by activating the PKC/ERK1/2 signaling pathway and provide Rab7b as a candidate to connect the PKC pathway and the ERK1/2 pathway in PMA-induced megakaryocytic differentiation of K562 cells. Disclosures: No relevant conflicts of interest to declare.
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Amos, John. "KAMPANYE GIZI SEIMBANG MELALUI KESENIAN RABAB." Jurnal Sehat Mandiri 13, no. 2 (2018): 35–41. http://dx.doi.org/10.33761/jsm.v13i2.25.
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ABSTRACT
 The prevalence of underweight children under five in Indonesia is still high, compared to the target of Indonesia's MDGs of 18.5% West Sumatra is still below the target. Health problems can not only be solved by the government, but community involvement is required, therefore the potential needs to be mobilized. Rabab is a traditional Minangkabau traditional string instrument made from coconut shell. With this rabab can be channeled one's musical talents. Usually in this rabab narrated a variety of nagari stories or known by the term Kaba.merupakan a traditional theater that is populist in Minangkabau area, West Sumatra. Research with qualitative approach with research strategy "Case Study Research (CSR)" with descriptive case study. The study was planned to be conducted on 10 (ten) rabab players active in the Balai Selasa, Pesisir Selatan District. Most informants stated that the balanced nutrition message can be compiled into story through Rabab, almost all informants stated that balanced nutrition messages can be prepared according to the language of the community local, most informants stated that the message of balanced nutrition can be compiled according to the dialeg minang community, most informants stated that the message of balanced nutrition can be compiled by summarizing information in simple form, Rabab as a regional art that has the potential Minangkabau have the potential to campaign balanced nutrition in achieve optimal health for children under five and family. It is recommended to review the balanced nutrition messages for the various ages of the community through Rabab art, to examine the influence of Rabab arts interventions on the knowledge, attitude and actions of people of all ages on balanced nutrition, to formulate strategies for the development of Rabab art through the preparation of scenarios for changes in community behavior about balanced nutrition.
 
 Keywords: nutrition, rabab
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Piper Hanley, Karen, Tom Hearn, Andrew Berry, et al. "In vitro expression of NGN3 identifies RAB3B as the predominant Ras-associated GTP-binding protein 3 family member in human islets." Journal of Endocrinology 207, no. 2 (2010): 151–61. http://dx.doi.org/10.1677/joe-10-0120.
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Neurogenin 3 (NGN3) commits pancreatic progenitors to an islet cell fate. We have induced NGN3 expression and identified upregulation of the gene encoding the Ras-associated small molecular mass GTP-binding protein, RAB3B. RAB3B localised to the cytoplasm of human β-cells, both during the foetal period and post natally. Genes encoding alternative RAB3 proteins and RAB27A were unaltered by NGN3 expression and in human adult islets their transcripts were many fold less prevalent than those of RAB3B. The regulation of insulin exocytosis in rodent β-cells and responsiveness to incretins are reliant on Rab family members, notably Rab3a and Rab27a, but not Rab3b. Our results support an important inter-species difference in regulating insulin exocytosis where RAB3B is the most expressed isoform in human islets.
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Hirsch, Thilo. "Rabab & Rebec." Schweizer Jahrbuch für Musikwissenschaft 39 (December 29, 2022): 163. http://dx.doi.org/10.36950/sjm.39.14.
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Overmeyer, Jean H., Amy L. Wilson, Robert A. Erdman, and William A. Maltese. "The Putative “Switch 2” Domain of the Ras-related GTPase, Rab1B, Plays an Essential Role in the Interaction with Rab Escort Protein." Molecular Biology of the Cell 9, no. 1 (1998): 223–35. http://dx.doi.org/10.1091/mbc.9.1.223.
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Posttranslational modification of Rab proteins by geranylgeranyltransferase type II requires that they first bind to Rab escort protein (REP). Following prenylation, REP is postulated to accompany the modified GTPase to its specific target membrane. REP binds preferentially to Rab proteins that are in the GDP state, but the specific structural domains involved in this interaction have not been defined. In p21 Ras, the α2 helix of the Switch 2 domain undergoes a major conformational change upon GTP hydrolysis. Therefore, we hypothesized that the corresponding region in Rab1B might play a key role in the interaction with REP. Introduction of amino acid substitutions (I73N, Y78D, and A81D) into the putative α2 helix of Myc-tagged Rab1B prevented prenylation of the recombinant protein in cell-free assays, whereas mutations in the α3 and α4 helices did not. Additionally, upon transient expression in transfected HEK-293 cells, the Myc-Rab1B α2 helix mutants were not efficiently prenylated as determined by incorporation of [3H]mevalonate. Metabolic labeling studies using [32P]orthophosphate indicated that the poor prenylation of the Rab1B α2 helix mutants was not directly correlated with major disruptions in guanine nucleotide binding or intrinsic GTPase activity. Finally, gel filtration analysis of cytosolic fractions from 293 cells that were coexpressing T7 epitope-tagged REP with various Myc-Rab1B constructs revealed that mutations in the α2 helix of Rab1B prevented the association of nascent (i.e., nonprenylated) Rab1B with REP. These data indicate that the Switch 2 domain of Rab1B is a key structural determinant for REP interaction and that nucleotide-dependent conformational changes in this region are largely responsible for the selective interaction of REP with the GDP-bound form of the Rab substrate.
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Anita, Yesi, Nurman Nurman, and Aldri Frinaldi. "NILAI-NILAI MORAL YANG TERDAPAT DALAM KABA PADA KESENIAN RABAB (Studi pada Sebuah Pertunjukan Kesenian Rabab di Nagari Duku Kecamatan Koto XI Tarusan Kabupaten Pesisir Selatan Sumatera Barat)." Humanus 11, no. 1 (2012): 52. http://dx.doi.org/10.24036/jh.v11i1.623.
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This research aims to reveal religious and moral values in kaba (a type of Saga in Minangkabau literature) and rabab Pasisie (a type of percussion in Minangkabau particularly the one from Pesisir Selatan regency). The research is qualitative descriptive. The data, primary and secondary, is collected by observation, interview, and documentation. The interviewees are selected through purposive sampling. The data is then tested by triangulation of the sources, and then analysed using selection, reduction, classification, and presentation of data, which is later concluded. The result shows that rabab Pasisie is still fancied by the communities in Nagari Duku Kecamatan Koto XI of Tarusan District. The research concludes that kaba and rabab Pasisie embodies moral values that can be applied in the daily life of the community. We suggest that the leader of Nagari Duku Kecamatan Koto XI of Tarusan District promulgate the traditional rabab as a consideration to make it cultural tourism, while the Minangkabau society can implement moral values in kaba, and it is important that the district’s youth continue to learn and play rabab to maintain the traditional arts. Key words: Minangkabau kaba, rabab Pasisie, moral values, arts
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Lu, Peilin, Donghua He, Yang Yang, et al. "Rab7b suppresses autophagy and induces apoptosis of K562 leukemic cells treated with homoharringtonine." Blood 118, no. 21 (2011): 4884. http://dx.doi.org/10.1182/blood.v118.21.4884.4884.
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Abstract Abstract 4884 Homoharringtonine is an effective anti-leukemia medicine developed by Chinese. It has been found to induce differentiation and apoptosis of leukemia cells. However, the mechanism of its anti-leukemia function has not been fully understood. Rab is a kind of G protein of Ras superfamily and participates in endocytosis and exocytosis of protein transport process. In recent studies, some Rab proteins such as Rab7 are found to be associated with cellular autophagy and apoptosis. We previously identified a new small GTPase homologous to Rab7, named Rab7b, which is selectively expressed in promyeloid and monocytic cells and is localized to lysosome-associated compartments. To investigate the roles of Rab7b in acute myeloid leukemia, we used leukemia cell line K562 as a model in the present study. After treatment of K562 cells with various doses of HHT, cell viability and apoptosis were measured by MTT assay and Annexin V/PI staining respectively. Protein expression of LC3, a marker of autophagy, and caspase3, 9, ERK1/2,Akt were determined by Western blot analysis. Using stable gene transfection, several Rab7b variants, including Rab7b wild-type, active mutant Rab7b-Q67L and localization-deficient mutant Rab7b-ΔCC as well as Rab7b RNAi were transfected in to K562 cells and their roles in regulation of apoptosis in K562 leukemia cells induced by HHT were further evaluated. Our data showed that the viability of the K562 cells was greatly reduced by HHT treatment in a dose- and time- dependant manner. Treatment of the K562 with HHT significantly increases apoptosis in the cells as measured by Annexin V/PI staining. Using Western blot analysis, we further determined that the expression of caspase3, 9 was increased, and ERK1/2 augmented with Akt was suppressed in the cells treated with HHT. After suppressing autophagy with 3-MA, apoptosis was enhanced in the K562 cells treated with HHT. (p<0.05). By constitutively expression of Rab7B and variants in K562 cells, we found that the rate of apoptotic cells are much higher in the K562 cells transfected with Rab7b wild-type and Rab7b-Q67L variants, along with increased expression of caspase3, 9, ERK1/2 and decreased expression of Akt in the transfectants with Rab7b wild-type and active mutant Rab7b-Q67L. Our study suggests that HHT is able to suppress autophagy and enhance apoptosis in K562 leukemia cells in a caspase-dependent way, which is associated with suppression of Akt phosphorylation and upregulation of ERK1/2. Over-expression of Rab7b can enhance HHT induced apoptosis in K562 cells, which may also be associated with suppression of Akt phosphorylation and upregulation of ERK1/2. Taken together, our study elucidates a new recognition for the mechanism of HHT in anti-leukemia therapy and provides a new insight into understanding the relationship between autophagy and apoptosis in leukemia cells induced chemotherapy. Disclosures: No relevant conflicts of interest to declare.
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Plutner, H., A. D. Cox, S. Pind, et al. "Rab1b regulates vesicular transport between the endoplasmic reticulum and successive Golgi compartments." Journal of Cell Biology 115, no. 1 (1991): 31–43. http://dx.doi.org/10.1083/jcb.115.1.31.
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We report an essential role for the ras-related small GTP-binding protein rab1b in vesicular transport in mammalian cells. mAbs detect rab1b in both the ER and Golgi compartments. Using an assay which reconstitutes transport between the ER and the cis-Golgi compartment, we find that rab1b is required during an initial step in export of protein from the ER. In addition, it is also required for transport of protein between successive cis- and medial-Golgi compartments. We suggest that rab1b may provide a common link between upstream and downstream components of the vesicular fission and fusion machinery functioning in early compartments of the secretory pathway.
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Weber, E., G. Berta, A. Tousson, et al. "Expression and polarized targeting of a rab3 isoform in epithelial cells." Journal of Cell Biology 125, no. 3 (1994): 583–94. http://dx.doi.org/10.1083/jcb.125.3.583.
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Pathways of polarized membrane traffic in epithelial tissues serve a variety of functions, including the generation of epithelial polarity and the regulation of vectorial transport. We have identified a candidate regulator of polarized membrane traffic in epithelial cells (i.e., rab3B), which is a member of the rab family of membrane traffic regulators. Rab3B is highly homologous to a brain-specific rab3 isoform (rab3A) that targets in a polarized fashion to the presynaptic nerve terminal, where it probably regulates exocytosis. The coding region for human rab3B was cloned from epithelial mRNA using a reverse-transcription polymerase chain reaction strategy. This cDNA clone hybridized to a single mRNA species in Northern blots of poly(A)+ RNA isolated from epithelial cell lines. A rab3B-specific antibody that was raised against recombinant fusion protein recognized a 25-kD band in immunoblots of cell lysates prepared from cultured epithelial cells (e.g., T84 and HT29-CL19A), but not from a variety of nonepithelial cells (e.g., PC12 neuroendocrine cells). Immunofluorescence analysis confirmed that rab3B protein is preferentially expressed in cultured epithelial cells as well as in a number of native epithelial tissues, including liver, small intestine, colon, and distal nephron. Rab3B localized to the apical pole very near the tight junctions between adjacent epithelial cells within all of these cell lines and native epithelial tissues, as determined by immunofluorescence and immunoelectron microscopic analysis. Moreover, this pattern of intracellular targeting was regulated by cell contact; namely, rab3B was reversibly retrieved from the cell periphery as epithelial cell contact was inhibited by reducing the extracellular Ca2+ concentration. Our results indicate that neurons and epithelial cells express homologous rab3 isoforms that target in a polarized fashion within their respective tissues. The pattern and regulation of rab3B targeting in epithelial cells implicates this monomeric GTPase as a candidate regulator of apical and/or junctional protein traffic in epithelial tissues.
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Armstrong, J., N. Thompson, J. H. Squire, J. Smith, B. Hayes, and R. Solari. "Identification of a novel member of the Rab8 family from the rat basophilic leukaemia cell line, RBL.2H3." Journal of Cell Science 109, no. 6 (1996): 1265–74. http://dx.doi.org/10.1242/jcs.109.6.1265.
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We describe the cloning of a cDNA from the rat basophilic leukaemia cell line (RBL.2H3) encoding a novel member of the Rab family of small GTP binding proteins. The novel clone, which we call Rab8b, is most highly related to the Rab8 family with substantial divergence in the variable C-terminal domain. Northern blot analysis reveals highest levels of expression of Rab8b in the spleen, testis and brain, which is in marked contrast to the tissue distribution of Rab8. The Rab8b cDNA was modified to introduce a c-myc epitope tag at the extreme N terminus of the protein, and transient transfection studies were performed to analyse the intracellular localization of Rab8b by confocal microscopy. Transient expression of the c-myc/Rab8b fusion protein in both PC12 and RBL.2H3 cells shows staining of both the plasma membrane and ill-defined vesicular structures, and in the case of RBL.2H3 cells appears to induce striking outgrowths of the plasma membrane.
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Alvarez, Cecilia, Rafael Garcia-Mata, Elizabeth Brandon, and Elizabeth Sztul. "COPI Recruitment Is Modulated by a Rab1b-dependent Mechanism." Molecular Biology of the Cell 14, no. 5 (2003): 2116–27. http://dx.doi.org/10.1091/mbc.e02-09-0625.
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The small GTPase Rab1b is essential for endoplasmic reticulum (ER) to Golgi transport, but its exact function remains unclear. We have examined the effects of wild-type and three mutant forms of Rab1b in vivo. We show that the inactive form of Rab1b (the N121I mutant with impaired guanine nucleotide binding) blocks forward transport of cargo and induces Golgi disruption. The phenotype is analogous to that induced by brefeldin A (BFA): it causes resident Golgi proteins to relocate to the ER and induces redistribution of ER-Golgi intermediate compartment proteins to punctate structures. The COPII exit machinery seems to be functional in cells expressing the N121I mutant, but COPI is compromised, as shown by the release of β-COP into the cytosol. Our results suggest that Rab1b function influences COPI recruitment. In support of this, we show that the disruptive effects of N121I can be reversed by expressing known mediators of COPI recruitment, the GTPase ARF1 and its guanine nucleotide exchange factor GBF1. Further evidence is provided by the finding that cells expressing the active form of Rab1b (the Q67L mutant with impaired GTPase activity) are resistant to BFA. Our data suggest a novel role for Rab1b in ARF1- and GBF1-mediated COPI recruitment pathway.
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Veeck, Christopher, Nadine Biedenkopf, Cornelius Rohde, Stephan Becker, and Sandro Halwe. "Inhibition of Rab1B Impairs Trafficking and Maturation of SARS-CoV-2 Spike Protein." Viruses 15, no. 4 (2023): 824. http://dx.doi.org/10.3390/v15040824.
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Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) utilizes cellular trafficking pathways to process its structural proteins and move them to the site of assembly. Nevertheless, the exact process of assembly and subcellular trafficking of SARS-CoV-2 proteins remains largely unknown. Here, we have identified and characterized Rab1B as an important host factor for the trafficking and maturation of the spike protein (S) after synthesis at the endoplasmic reticulum (ER). Using confocal microscopy, we showed that S and Rab1B substantially colocalized in compartments of the early secretory pathway. Co-expression of dominant-negative (DN) Rab1B N121I leads to an aberrant distribution of S into perinuclear spots after ectopic expression and in SARS-CoV-2-infected cells caused by either structural rearrangement of the ERGIC or Golgi or missing interaction between Rab1B and S. Western blot analyses revealed a complete loss of the mature, cleaved S2 subunit in cell lysates and culture supernatants upon co-expression of DN Rab1B N121I. In sum, our studies indicate that Rab1B is an important regulator of trafficking and maturation of SARS-CoV-2 S, which not only improves our understanding of the coronavirus replication cycle but also may have implications for the development of antiviral strategies.
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White, Richard C., and Nicholas P. Cianciotto. "Type II Secretion Is Necessary for Optimal Association of the Legionella-Containing Vacuole with Macrophage Rab1B but Enhances Intracellular Replication Mainly by Rab1B-Independent Mechanisms." Infection and Immunity 84, no. 12 (2016): 3313–27. http://dx.doi.org/10.1128/iai.00750-16.
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Previously, we documented that type II secretion (T2S) promotes intracellular infection of macrophages byLegionella pneumophila. In the present study, we identified infection events that are modulated by T2S by comparing the behaviors of wild-type and T2S mutant bacteria in murine bone marrow-derived macrophages and human U937 cells. Although the two strains behaved similarly for entry into the host cells and evasion of lysosomal fusion, the mutant was impaired in the ability to initiate replication between 4 and 8 h postentry and to grow to large numbers in theLegionella-containing vacuole (LCV), as evident at 12 h. At 4 h postinoculation, mutant LCVs had a significantly reduced association with Rab1B, a host GTPase that facilitates the tethering of endoplasmic reticulum (ER)-derived vesicles to LCVs. The mutant did not lose expression or translocation of six type IV secretion effectors (e.g., SidM) that are well known for mediating Rab1B association with the LCV, indicating that T2S promotes the interaction between the LCV and Rab1B via a novel mechanism. Interestingly, the mutant's growth defect was exacerbated in macrophages that had been depleted of Rab1B by short hairpin RNA (shRNA) treatment, indicating that T2S also potentiates events beyond Rab1B association. In support of this, asidM lspFdouble mutant had an intracellular growth defect that was more dramatic than that of thelspFmutant (and asidMmutant) and showed a growth difference of as much as a 400-fold compared to the wild type. Together, these data reveal a new role for T2S in intracellular infection that involves both Rab1B-dependent and Rab1B-independent processes.
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LIN, Chong-Gee, Yu-Chi LIN, Hwan-Wun LIU, and Lung-Sen KAO. "Characterization of Rab3A, Rab3B and Rab3C: different biochemical properties and intracellular localization in bovine chromaffin cells." Biochemical Journal 324, no. 1 (1997): 85–90. http://dx.doi.org/10.1042/bj3240085.
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In this study we examined the biochemical properties and subcellular localization of Rab3A, Rab3B and Rab3C in bovine adrenal chromaffin cells. The Kd for guanosine 5′-[γ-thio]triphosphate (GTP[S]) of the three Rab3 proteins was 15, 2700 and 204 nM for Rab3A, Rab3B and Rab3C respectively. The intrinsic GTPase activity of the three Rab3 proteins seemed similar and was increased approx. 3-fold by bovine chromaffin cell lysate. Truncation of the C-terminal 31 amino acid residues decreased the binding affinity for GTP[S] of the three Rab3 proteins. When the C-terminus of Rab3C was replaced with that of Rab3A, the binding affinity of Rab3C for GTP[S] was decreased, but the replacement did not affect the affinity of Rab3B for GTP[S]. Immunostaining experiments showed that Rab3A, Rab3B and Rab3C are localized separately within chromaffin cells. Anti-Rab3A and anti-Rab3C antibodies stained vesicle-like structures, whereas anti-Rab3B antibody distinctly stained the plasma membrane. In summary, bovine chromaffin cells express the three Rab3 proteins but the subcellular localization and biochemical properties of the three Rab3 proteins are distinct.
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Zou, Liyun, Jingran Zhou, Jinyu Zhang, et al. "The GTPase Rab3b/3c-positive recycling vesicles are involved in cross-presentation in dendritic cells." Proceedings of the National Academy of Sciences 106, no. 37 (2009): 15801–6. http://dx.doi.org/10.1073/pnas.0905684106.
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Antigen cross-presentation in dendritic cells is a complex intracellular membrane transport process, but the underlying molecular mechanisms remain to be thoroughly investigated. In this study, we examined the effect of siRNA-mediated knockdown of 57 Rab GTPases, the key regulators of membrane trafficking, on antigen cross-presentation. Twelve Rab GTPases were identified to be associated with antigen cross-presentation, and Rab3b/3c was indicated to be colocalized with MHC class I molecules at perinuclear tubular structure. Tracing with fluorescence protein-tagged β2-microglobulin demonstrated that the MHC class I molecules were internalized from the plasma membrane to Rab3b/3c-positive compartments, which were also colocalized with the internalized transferrin. Moreover, depletion of Rab3b/3c strongly reduced the fast phase recycling rate of transferrin receptors. Furthermore, the Rab3b/3c-positive compartments were colocalized with a fraction of Rab27a at a juxtaposition of phagosomes. Together, these data demonstrate that Rab3b/3c-positive recycling vesicles are involved in and may constitute one of the recycling compartments in exogenous antigen cross-presentation.
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Kamal, Zahara. "NYANYIAN ANAK BALAM: TERAPI MISTIK PERDUKUNAN KE SENI PERTUNJUKAN RABAB PASISIE DI PESISIR SELATAN SUMATERA BARAT." Humanus 14, no. 2 (2015): 165. http://dx.doi.org/10.24036/jh.v14i2.5683.
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This article is part of reserch,which reveals the problem of singing of anak balam in the context of shamanism entered the real of the performing art tradition of rabab pasisie in south pesisir.in the context of shamanism,the singing of anak balam serves as a means of communication between shamans and spirit of ancestor to identify a patient’s disease and type of medicine.while the singing of anak balam in context of performing arts rabab, serves as ameans of intertainment to enliven various local community activities.this reserch use a quanlitative method sosiology approach,antropology approach,easthetic approach.data colected through observasion, interview and documentation.this research found that in term of aesthetics singing of anak balam in context of shamanic different with the singing of anak balam in the context of art performing rabab pasisie, because in shamanism context eathetic value is not considered by shamans, because preferred here is to communicate with the spirit of ancestors,while in the context of performing arts rabab,aesthitic value higly considered by rabab artists both from the cultivation of musical and singing text.
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Adha Zuhkri Arafad and Budiwirman Budiwirman. "Perancangan Buku Ilustrasi “Nyanyian Anak Balam Rabab Pasisia“." Student Research Journal 1, no. 5 (2023): 132–44. http://dx.doi.org/10.55606/srjyappi.v1i5.632.
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The Balam Rabab Pasisia Children's Song is a folk tale from the Minangkabau people, West Sumatra, South Coast. It is said that the children's singing of Balam is a traditional healing process. In the past, someone from the family who was sick would be taken to a shaman (smart person) to carry out a healing ritual. In line with the development of the Balam Children's Song era, it has now entered the Pesisian rabab performing arts. Besides that, rabab is a performing art that tells stories about kaba from other folk tales. This is what prompted the designer to design the illustrated book ''Nyaniyan Anak Balam Rabab Pasisia''. There are still many among the younger generation who do not know the culture, traditions and folklore because there is a lack of information and dissemination is still not effective. The aim of designing the illustration book "Song of Children's Balam Rabab Pasisia" is to provide information to the Minangkabau community about the story of the Song of Children's Balam Rabab Pasisia, as well as a medium for preserving folklore in Minangkabau society. The design of the illustration "Balam Rabab Pasisia Children's Song" uses the glass box design method with the 5W1H analysis technique. The type of illustration used is a 2D semi-realist cartoon. The output of this design is a book measuring 18 cm x 18 cm which contains narrative and related illustrations to clarify the narrative and also add to the aesthetic value of the book. After carrying out a feasibility test, the results showed that the design was good starting from the colors, illustrations, fonts and layout. And it can be concluded that the Illustrated Book "Children's Songs of Balam Rabab Pasisia" was well received by respondents. In this design there is also supporting media which includes: posters, banners, bookmarks, e-books, t-shirts, tote bags, key chains, tumblers and stickers.
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Darmansyah, Darmansyah. "Perilaku Bermusik dalam Repertoar Rabab Pasisia Kaba Gadih Basanai." Jurnal Sendratasik 12, no. 2 (2023): 220. http://dx.doi.org/10.24036/js.v12i2.122978.
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Kaba Gadih Basanai is a very popular story for the Minangkabau people and gives a very deep meaning in life because there is a historical background to the life of the people in the colonial era which was full of twists and turns of heartbreaking struggles. The purpose of this study is to examine how music behaves in conveying Rabab Pasisie kaba Gadih Basanai. This research is research with a descriptive qualitative approach, the researcher makes observations, describes then gives meaning to the symbols found. The Gadih Basanai song has traditional musical elements played by all the artists all of these artists have a different musical taste for the quality of each talent they have, and different characteristics when the performers of the Rabab (Violin) arts serve it. The method used in this study is a qualitative method with a descriptive analysis approach. Musical behaviour is presented in various forms and musical variants created by each Rabab (violin) maker based on the artist's level of intelligence, to be able to give a musical sense that is presented during performances of Gadih Basanai singing or songs. Then the behaviour of the Rabab makers seems clear that the Rabab which is played through the instrument instrumental melodies often give or brings up new melodies through inspiration by the players themselves, who always synergize between instruments to become an interesting work and not out of frames the melody on the coastal Rabab itself.
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Bucci, Cecilia, Oddmund Bakke, and Cinzia Progida. "Rab7b and receptors trafficking." Communicative & Integrative Biology 3, no. 5 (2010): 401–4. http://dx.doi.org/10.4161/cib.3.5.12341.
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Jalagadugula, Gauthami, Lawrence E. Goldfinger, Guangfen Mao, Michele P. Lambert, and A. Koneti Rao. "Defective RAB1B-related megakaryocytic ER-to-Golgi transport in RUNX1 haplodeficiency: impact on von Willebrand factor." Blood Advances 2, no. 7 (2018): 797–806. http://dx.doi.org/10.1182/bloodadvances.2017014274.
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Key Points GTPase RAB1B is a direct transcriptional target of RUNX1 in MK/platelets and is downregulated in RUNX1 haplodeficiency. RUNX1 downregulation is associated with defective RAB1B-related ER-to-Golgi transport and alterations in α-granule vWF.
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Kato, Yukino, Remina Shirai, Katsuya Ohbuchi, et al. "Hesperetin Ameliorates Inhibition of Neuronal and Oligodendroglial Cell Differentiation Phenotypes Induced by Knockdown of Rab2b, an Autism Spectrum Disorder-Associated Gene Product." Neurology International 15, no. 1 (2023): 371–91. http://dx.doi.org/10.3390/neurolint15010025.
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Autism spectrum disorder (ASD) is a central nervous system (CNS) neurodevelopmental disorder that includes autism, pervasive developmental disorder, and Asperger’s syndrome. ASD is characterized by repetitive behaviors and social communication deficits. ASD is thought to be a multifactorial disorder with a range of genetic and environmental factors/candidates. Among such factors is the rab2b gene, although it remains unclear how Rab2b itself is related to the CNS neuronal and glial developmental disorganization observed in ASD patients. Rab2 subfamily members regulate intracellular vesicle transport between the endoplasmic reticulum and the Golgi body. To the best of our knowledge, we are the first to report that Rab2b positively regulates neuronal and glial cell morphological differentiation. Knockdown of Rab2b inhibited morphological changes in N1E-115 cells, which are often used as the neuronal cell differentiation model. These changes were accomplished with decreased expression levels of marker proteins in neuronal cells. Similar results were obtained for FBD-102b cells, which are used as the model of oligodendroglial cell morphological differentiation. In contrast, knockdown of Rab2a, which is another Rab2 family member not known to be associated with ASD, affected only oligodendroglial and not neuronal morphological changes. In contrast, treatment with hesperetin, a citrus flavonoid with various cellular protective effects, in cells recovered the defective morphological changes induced by Rab2b knockdown. These results suggest that knockdown of Rab2b inhibits differentiation in neuronal and glial cells and may be associated with pathological cellular phenotypes in ASD and that hesperetin can recover their phenotypes at the in vitro level at least.
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Fernandez-Chamorro, Javier, Rosario Francisco-Velilla, Jorge Ramajo, and Encarnación Martinez-Salas. "Rab1b and ARF5 are novel RNA-binding proteins involved in FMDV IRES–driven RNA localization." Life Science Alliance 2, no. 1 (2019): e201800131. http://dx.doi.org/10.26508/lsa.201800131.
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Internal ribosome entry site (IRES) elements are organized in domains that guide internal initiation of translation. Here, we have combined proteomic and imaging analysis to study novel foot-and-mouth disease virus IRES interactors recognizing specific RNA structural subdomains. Besides known picornavirus IRES–binding proteins, we identified novel factors belonging to networks involved in RNA and protein transport. Among those, Rab1b and ARF5, two components of the ER-Golgi, revealed direct binding to IRES transcripts. However, whereas Rab1b stimulated IRES function, ARF5 diminished IRES activity. RNA-FISH studies revealed novel features of the IRES element. First, IRES-RNA formed clusters within the cell cytoplasm, whereas cap-RNA displayed disperse punctate distribution. Second, the IRES-driven RNA localized in close proximity with ARF5 and Rab1b, but not with the dominant-negative of Rab1b that disorganizes the Golgi. Thus, our data suggest a role for domain 3 of the IRES in RNA localization around ER-Golgi, a ribosome-rich cellular compartment.
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Okprioni, Vivin, and Harisnal Hadi. "PEMBELAJARAN INSTRUMEN MUSIK TRADISIONAL RABAB KELAS X DI SMK NEGERI 7 PADANG." Jurnal Sendratasik 8, no. 4 (2019): 37. http://dx.doi.org/10.24036/jsu.v7i4.105106.
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Abstract This article aims to know the process of learning music rabab against learning styles students use audio visual media in SMK Padang 7. This type of research is qualitative descriptive method. Object of research i.e. grade X Karawitan SMKN 7 Padang. The data obtained is collected through literature study, observation, interviews, and documentation. Results of the study is that the results of student learning is already quite good than ever before. Based on the results of the observation of learning styles, media relevant to learning is applied in the audiovisual media. Lying spirit and students in learning the music improved rabab. Students are more motivated to follow the music learning process of learning from the rabab proved that followed with active students analyze, follow, move, touches and bold things in regard to learning music rabab. It also can be proved by the increase in student learning outcomes assessment. Keywords: learning, learning styles, and audio visual media
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Nagata, Koh-ichi, Yukio Okano, and Yoshinori Nozawa. "Differential Expression of Low Mr GTP-binding Proteins in Human Megakaryoblastic Leukemia Cell Line, MEG-01, and their Possible Involvement in the Differentiation Process." Thrombosis and Haemostasis 77, no. 02 (1997): 368–75. http://dx.doi.org/10.1055/s-0038-1655970.
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SummaryThe expression of various low Mr GTP-binding proteins at various states of differentiation of a human megakaryoblastic leukemia cell line, MEG-01, was analyzed using thermocycle amplification of mRNA and immunoblotting. MEG-01 cells were found to express mRNAs of rap1A, rap1B, rap2B, ralA, rhoA, rac1, rac2, CDC42Hs, rab1, rab3B, rab6, ram and ran, but not rab4, and the proteins of Rap 1, Rap2, RhoA, Rac1, Rac2, Rab3B, Rab4, Rab6 and Rab8 were expressed. Differentiation of MEG-01 cells induced by 100 nM 12-O-tetradecanoylphorbol-13-acetate revealed the considerable increases in mRNA expression of rap1B, rab3B, rabA, ram and ran whereas the levels of rap2B, rhoA and rac1 decreased. During the differentiation process, significant changes in protein levels of Rap1, RhoA, Rac1, Rac2, Rab3B, Rab4 and Rab6 were observed among three subcellular (cytosol, Triton X-100-soluble membrane and -insoluble cytoskeleton) fractions. The present investigation may be useful for the study of the megakaryocyte differentiation.
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Gyurkovska, Valeriya, Rakhilya Murtazina, Sarah F. Zhao, Sojin Shikano, Yukari Okamoto, and Nava Segev. "Dual function of Rab1A in secretion and autophagy: hypervariable domain dependence." Life Science Alliance 6, no. 5 (2023): e202201810. http://dx.doi.org/10.26508/lsa.202201810.
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We currently understand how the different intracellular pathways, secretion, endocytosis, and autophagy are regulated by small GTPases. In contrast, it is unclear how these pathways are coordinated to ensure efficient cellular response to stress. Rab GTPases localize to specific organelles through their hypervariable domain (HVD) to regulate discrete steps of individual pathways. Here, we explored the dual role of Rab1A/B (92% identity) in secretion and autophagy. We show that although either Rab1A or Rab1B is required for secretion, Rab1A, but not Rab1B, localizes to autophagosomes and is required early in stress-induced autophagy. Moreover, replacing the HVD of Rab1B with that of Rab1A enables Rab1B to localize to autophagosomes and regulate autophagy. Therefore, Rab1A-HVD is required for the dual functionality of a single Rab in two different pathways: secretion and autophagy. In addition to this mechanistic insight, these findings are relevant to human health because both the pathways and Rab1A/B were implicated in diseases ranging from cancer to neurodegeneration.
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Abenza, Juan F., Antonio Galindo, Areti Pantazopoulou, Concha Gil, Vivian de los Ríos, and Miguel A. Peñalva. "Aspergillus RabBRab5 Integrates Acquisition of Degradative Identity with the Long Distance Movement of Early Endosomes." Molecular Biology of the Cell 21, no. 15 (2010): 2756–69. http://dx.doi.org/10.1091/mbc.e10-02-0119.
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Aspergillus nidulans early endosomes display characteristic long-distance bidirectional motility. Simultaneous dual-channel acquisition showed that the two Rab5 paralogues RabB and RabA colocalize in these early endosomes and also in larger, immotile mature endosomes. However, RabB-GTP is the sole recruiter to endosomes of Vps34 PI3K (phosphatidylinositol-3-kinase) and the phosphatidylinositol-3-phosphate [PI(3)P] effector AnVps19 and rabBΔ, leading to thermosensitivity prevents multivesicular body sorting of endocytic cargo. Thus, RabB is the sole mediator of degradative endosomal identity. Importantly, rabBΔ, unlike rabAΔ, prevents early endosome movement. As affinity experiments and pulldowns showed that RabB-GTP recruits AnVps45, RabB coordinates PI(3)P-dependent endosome-to-vacuole traffic with incoming traffic from the Golgi and with long-distance endosomal motility. However, the finding that Anvps45Δ, unlike rabBΔ, severely impairs growth indicates that AnVps45 plays RabB-independent functions. Affinity chromatography showed that the CORVET complex is a RabB and, to a lesser extent, a RabA effector, in agreement with GST pulldown assays of AnVps8. rabBΔ leads to smaller vacuoles, suggesting that it impairs homotypic vacuolar fusion, which would agree with the sequential maturation of endosomal CORVET into HOPS proposed for Saccharomyces cerevisiae. rabBΔ and rabAΔ mutations are synthetically lethal, demonstrating that Rab5-mediated establishment of endosomal identity is essential for A. nidulans.
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Mitchell, Joseph C., Joseph R. Mendelson, and Margaret M. Stewart. "George Bernard Rabb." Copeia 103, no. 4 (2015): 1086–92. http://dx.doi.org/10.1643/ot-15-361.
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Mitchell, Joseph C., Joseph R. Mendelson, and Margaret M. Stewart. "George Bernard Rabb." Copeia 105, no. 3 (2017): 592–98. http://dx.doi.org/10.1643/ot-15-361.1.
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He, Donghua, Taoyong Chen, Mingjing Yang, Xuhui Zhu, Xuetao Cao, and Zhen Cai. "Small Rab GTPase Rab7b Promotes Megakaryocytic Differentiation by Enhancing IL-6 Production and STAT3-GATA-1 Association." Blood 116, no. 21 (2010): 1549. http://dx.doi.org/10.1182/blood.v116.21.1549.1549.
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Abstract Abstract 1549 Interleukin 6 (IL-6) is a pleiotropic cytokine acting on a variety of cell types, and plays important roles in hematopoiesis. GATA-1 (GATA binding protein 1) is an important transcription factor involved in either megakaryocytic or erythrocytic differentiation. However, the mechanisms for IL-6-induced megakaryocytic differentiation and regulation of GATA-1 have not been fully elucidated. By using phorbol-12-myristate-13-acetate (PMA)-induced megakaryocytic differentiation of K562 cells as a model, we investigated the roles of Rab7b, a late endosome/lysosome-localized myeloid small GTPase, in megakaryocytic differentiation. We find that Rab7b can promote PMA-induced megakaryocytic differentiation, as evidenced by typical morphological alterations, increased fibronectin-specific adhesion, increased polyploidy formation, and increased expression of CD41a. The GTP-bound status and lysosomal localization of Rab7b are required for promotion of K562 megakaryocytic differentiation, which can be blocked by inhibitor of nuclear factor κB (NF-κB) and neutralizing antibodies for IL-6 and gp130. In Rab7b-silenced K562 cells, PMA-induced activation of NF-κB, IL-6 production and megakaryocytic differentiation are impaired. Furthermore, we demonstrate that IL-6-induced activation of signal transducer and activator of transcription 3 (STAT3) and the subsequent association of STAT3 with GATA-1 may contribute to PMA-induced and Rab7b-mediated transcriptional upregulation of megakaryocytic differentiation markers. Therefore, our data suggest that Rab7b may play important roles in megakaryopoiesis by activating NF-κB and promoting IL-6 production. Our study also indicates that the IL-6-induced association of STAT3 with GATA-1 may favor megakaryopoiesis. Disclosures: No relevant conflicts of interest to declare.
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Chen, Xiubing, Xiaomin Liao, Biaolin Zheng, et al. "Differential Plasma Proteins Identified via iTRAQ-Based Analysis Serve as Diagnostic Markers of Pancreatic Ductal Adenocarcinoma." Disease Markers 2023 (January 20, 2023): 1–11. http://dx.doi.org/10.1155/2023/5145152.
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Objective. We aimed to identify differentially expressed proteins in the plasma of patients with pancreatic cancer and control subjects, which could serve as potential tumor biomarkers. Methods. Differentially expressed proteins were determined via isostatic labeling and absolute quantification (iTRAQ). Potential protein biomarkers were identified via enzyme-linked immunosorbent assay (ELISA) in 40 patients and 40 control subjects, and those eventually selected were further validated in 40 pancreatic cancer and normal pancreatic tissues. Results. In total, 30 proteins displayed significant differences in expression among which 21 were downregulated and 9 were upregulated compared with the control group. ELISA revealed downregulation of peroxiredoxin-2 (PRDX2) and upregulation of alpha-1-antitrypsin (AAT), Ras-related protein Rab-2B (RAB2B), insulin-like growth factor-binding protein 2 (IGFBP2), Rho-related GTP-binding protein RhoC (RHOC), and prelamin-A/C (LMNA) proteins in 40 other samples of pancreatic cancer. Notably, only AAT, RAB2B, and IGFBP2 levels were consistent with expression patterns obtained with iTRAQ. Moreover, all three proteins displayed a marked increase in pancreatic cancer tissues. Data from ROC curve analysis indicated that the diagnostic ability of AAT, RAB2B, and IGFBP2 combined with carbohydrate antigen 19-9 (CA19-9) for pancreatic cancer was significantly greater than that of the single indexes (area under the curve (AUC): 90% vs. 75% (CA19-9), 76% (AAT), 71% (RAB2B), and 71% (IGFBP2), all P < 0.01 ). Conclusion. AAT, RAB2B, and IGFBP2 could serve as effective biomarkers to facilitate the early diagnosis of pancreatic cancer.
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Hartitom, Hartitom. "Rabab Pasisia sebagai Pertunjukan Seni Tutur di Kabupaten Pesisir Selatan." Resital: Jurnal Seni Pertunjukan 20, no. 1 (2019): 1–12. http://dx.doi.org/10.24821/resital.v20i1.2588.
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Penelitian ini sampai pada kesimpulan bahwa keberlangsungan dan perubahan pada pertunjukan Rabab Pesisir Selatan, terutama sekali di wilayah Lengayang telah meluas menjadi suatu bentuk seni multifungsi. Nyanyian Sikambang merupakan ungkapan bermakna komunal bagi masyarakat Pesisir Selatan, yang memiliki makna filosofi kehidupan dan pengertian lokal tertentu. Beberapa gaya seni populer ditemukan pula sebagai bagian yang terintegrasi dalam pertunjukan musik tradisi ini. Tujuannya adalah untuk tetap memiliki daya tarik di tengah masyarakat Sumatera Barat dan merupakan pula satu bentuk cara bertahan hidup bagi para seniman dan masyarakat pendukung kesenian Pesisir Selatan. Rabab Pasisia as a Tutur Art Show in The Pesisir Selatan District. The research has come-up with the conclusion that cotinuity and change in Pesisir Selatan rabab performance, particularly in sub-district of Lengayang has been expanded to a multifunctional arts. Sikambang song has become a means of communal expression in Pesisir Selatan, with its certain local meanings and philosophy of life. Some genre of popular-art has been found-out as integrated in the musical style, in order to maintain attractiveness amongst the West Sumatera community and survival for its musicians and its Pesisir Selatan supporting society.Keywords: rabab; pasisia; sikambang; tutur
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Campoy, Emanuel Martín, Felipe Carlos Martín Zoppino, and María Isabel Colombo. "The Early Secretory Pathway Contributes to the Growth of theCoxiella-Replicative Niche." Infection and Immunity 79, no. 1 (2010): 402–13. http://dx.doi.org/10.1128/iai.00688-10.
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ABSTRACTCoxiella burnetiiis a Gram-negative obligate intracellular bacterium. After internalization, this bacterium replicates in a large parasitophorous vacuole that has features of both phagolysosomes and autophagosomal compartments. We have previously demonstrated that early after internalizationCoxiellaphagosomes interact with both the endocytic and the autophagic pathways. In this report, we present evidence that theCoxiella-replicative vacuoles (CRVs) also interact with the secretory pathway. Rab1b is a small GTPase responsible for the anterograde transport between the endoplasmic reticulum and the Golgi apparatus. We present evidence that Rab1b is recruited to the CRV at later infection times (i.e., after 6 h of infection). Interestingly, knockdown of Rab1b altered vacuole growth, indicating that this protein was required for the proper biogenesis of the CRV. In addition, overexpression of the active GTPase-defective mutant (GFP-Rab1b Q67L) affected the development of theCoxiella-replicative compartment inhibiting bacterial growth. On the other hand, disruption of the secretory pathway by brefeldin A treatment or by overexpression of Sar1 T39N, a defective dominant-negative mutant of Sar1, affected the typical spaciousness of the CRVs. Taken together, our results show for the first time that theCoxiella-replicative niche also intercepts the early secretory pathway.
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Kulkarni, Rewa, Maria E. Teves, Angela X. Han, Jan M. McAllister, and Jerome F. Strauss. "Colocalization of Polycystic Ovary Syndrome Candidate Gene Products in Theca Cells Suggests Novel Signaling Pathways." Journal of the Endocrine Society 3, no. 12 (2019): 2204–23. http://dx.doi.org/10.1210/js.2019-00169.
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Abstract Genome-wide association studies identified loci associated with polycystic ovary syndrome (PCOS), including those near the LH receptor gene (LHCGR), a clathrin-binding protein (DENND1A) that functions as a guanine nucleotide exchange factor, and the gene encoding RAB5B, a GTPase involved in vesicular trafficking. We proposed that these three PCOS loci could be assembled into a functional network that contributes to altered gene expression in theca cells, resulting in increased androgen synthesis. The functional significance of this network was supported by our discovery that a truncated protein splice variant of the DENND1A gene, termed DENND1A.V2, is elevated in PCOS theca cells, and that forced expression of DENND1A.V2 in normal theca cells increased CYP11A1 and CYP17A1 expression and androgen synthesis, a hallmark of PCOS. In this study, we demonstrate the colocalization of LHCGR, DENND1AV.2, and RAB5B proteins in various cellular compartments in normal and PCOS theca cells by immunofluorescence. Human chorionic gonadotropin and forskolin stimulation was shown to affect the cytoplasmic distribution of LHCGR, DENND1A.V2, and RAB5B. DENND1A.V2 accumulated in the nuclei of the theca cells. Moreover, PCOS theca cells, following forskolin treatment, had a significantly greater relative abundance of nuclear DENND1A.V2. RAB5B also accumulated in the nuclei of PCOS theca cells treated with forskolin. In contrast, LHCGR did not enter the nucleus. This cytological evidence, and the previously reported increase in androgen biosynthesis with forced expression of DENND1A.V2 in normal theca cells, raises the possibility that DENND1A.V2 and RAB5B participate in increasing transcription of genes involved in androgen synthesis.
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Ballester, Jordi. "Influencias del rebec europeo sobre el rabel hispánico a finales de la Edad Media en la Corona de Aragón." Anuario Musical, no. 60 (December 30, 2005): 21. http://dx.doi.org/10.3989/anuariomusical.2005.60.48.
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Fruto de la fusión entre la lyra bizantina y el rabab árabe surgió y se desarrolló en la Europa medieval el rebec europeo. Sin embargo, diversas fuentes iconográficas parecen indicar que la música hispana -al menos por lo que a la antigua Corona de Aragón se refiere- se mantuvo ajena a ese tipo de rebec hasta muy avanzado el siglo XV, conservando entre sus instrumentos un tipo de rabel estrechamente emparentado con el rabab árabe. A partir de fuentes iconográficas y documentos, el presente artículo adelanta ligeramente la cronología mencionada, y muestra cómo -en la Corona de Aragón- los contactos entre el rebec europeo y el rabel hispánico (tipo rabab) se iniciaron ya hacia la segunda mitad del siglo XIV (o durante la primera mitad del siglo XV).
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Wang, Yuzhen, Taoyong Chen, Chaofeng Han, et al. "Lysosome-associated small Rab GTPase Rab7b negatively regulates TLR4 signaling in macrophages by promoting lysosomal degradation of TLR4." Blood 110, no. 3 (2007): 962–71. http://dx.doi.org/10.1182/blood-2007-01-066027.
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Abstract Toll-like receptor 4 (TLR4) initiates both myeloid differentiation factor 88 (MyD88)-dependent and Toll/interleukin (IL)-1R domain–containing adapter, inducing interferon (IFN)-β–dependent signaling, leading to production of proinflammatory mediators and type I interferon (IFN) to eliminate pathogens. However, uncontrolled TLR4 activation may contribute to pathogenesis of autoimmune and inflammatory diseases. TLR4 is transported from the plasma membrane to the endosome for ubiqutination and to the lysosome for degradation, and downregulation of TLR4 expression or promotion of TLR4 degradation are important ways for negative regulation of TLR4 signaling. We previously identified a lysosome-associated small guanosine triphosphatase (GTPase) Rab7b that may be involved in lysosomal trafficking and degradation of proteins. Here we demonstrate that Rab7b can negatively regulate lipopolysaccharide (LPS)-induced production of tumor necrosis factor (TNF)-α, IL-6, nitric oxide, and IFN-β, and potentiate LPS-induced activation of mitogen-activated protein kinase, nuclear factor κB, and IFN regulatory factor 3 signaling pathways in macrophages by promoting the degradation of TLR4. Rab7b is localized in LAMP-1–positive subcellular compartments and colocalized with TLR4 after LPS treatment and can decrease the protein level of TLR4. Our findings suggest that Rab7b is a negative regulator of TLR4 signaling, potentially by promoting the translocation of TLR4 into lysosomes for degradation.
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Bierings, Ruben, Nicola Hellen, Nikolai Kiskin, et al. "The interplay between the Rab27A effectors Slp4-a and MyRIP controls hormone-evoked Weibel-Palade body exocytosis." Blood 120, no. 13 (2012): 2757–67. http://dx.doi.org/10.1182/blood-2012-05-429936.
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Abstract Weibel-Palade body (WPB) exocytosis underlies hormone-evoked VWF secretion from endothelial cells (ECs). We identify new endogenous components of the WPB: Rab3B, Rab3D, and the Rab27A/Rab3 effector Slp4-a (granuphilin), and determine their role in WPB exocytosis. We show that Rab3B, Rab3D, and Rab27A contribute to Slp4-a localization to WPBs. siRNA knockdown of Slp4-a, MyRIP, Rab3B, Rab3D, Rab27A, or Rab3B/Rab27A, or overexpression of EGFP-Slp4-a or EGFP-MyRIP showed that Slp4-a is a positive and MyRIP a negative regulator of WPB exocytosis and that Rab27A alone mediates these effects. We found that ECs maintain a constant amount of cellular Rab27A irrespective of the WPB pool size and that Rab27A (and Rab3s) cycle between WPBs and a cytosolic pool. The dynamic redistribution of Rab proteins markedly decreased the Rab27A concentration on individual WPBs with increasing WPB number per cell. Despite this, the probability of WPB release was independent of WPB pool size showing that WPB exocytosis is not determined simply by the absolute amount of Rab27A and its effectors on WPBs. Instead, we propose that the probability of release is determined by the fractional occupancy of WPB-Rab27A by Slp4-a and MyRIP, with the balance favoring exocytosis.
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Ruíz Moleón, Vera, Maryam Fotouhi, Riham Ayoubi, et al. "A guide to selecting high-performing antibodies for Rab1A and Rab1B for use in Western Blot, immunoprecipitation and immunofluorescence." F1000Research 12 (December 28, 2023): 1578. http://dx.doi.org/10.12688/f1000research.143928.2.
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Rab1 is a highly conserved small GTPase that exists in humans as two isoforms: Rab1A and Rab1B, sharing 92% sequence identity. These proteins regulate vesicle trafficking between the endoplasmic reticulum (ER) and Golgi and within the Golgi stacks. Rab1A and Rab1B may be oncogenes, as they are frequently dysregulated in various human cancers. Moreover, they contribute to the progression of Parkinson’s disease. The availability of high-quality antibodies specific for Rab1A or Rab1B is essential to understand the distinct functions of these Rab1 proteins in both health and diseaseand to enhance the reproducibility of research involving these proteins. In this study, we characterized seven antibodies targeting Rab1A and five antibodies targeting Rab1B for Western Blot, immunoprecipitation, and immunofluorescence using a standardized experimental protocol based on comparing read-outs in knockout cell lines and isogenic parental controls. These studies are part of a much larger, collaborative initiative seeking to address the antibody reproducibility issue by characterizing commercially available antibodies for human proteins and publishing the results openly as a valuable resource for the scientific community. While uses of antibodies and protocols vary between laboratories, we encourage readers to use this report as a guide to select the most appropriate antibodies for their specific needs.