Dissertations / Theses on the topic 'RabbitMQ'

The main goal of this project has been to investigate how a message queue can be used to handle error codes in log files more actively. The project has followed the Design Science Research Methodology for development and implementation of the solution. A model of the transaction system was developed and emulated in newly developed applications. Two experiments were performed, the first of which tested a longer run time with intervals between messages and the second a time measurement of how long it takes to send 20 000 messages. The first experiment showed that the message queue was able to handle all messages which gave a high throughput of 22.5 messages per second without any messages being lost. The implemented consumer application received all messages and successfully counted the number of error codes in the received data. The experiments that have been carried out have proven that a message queue can be implemented to handle error codes in log files more actively. The future work that can be performed may include an evaluation of the security of the system, comparisons of performance compared to other message queues, performing the experiments on more powerful computers and implementation of machine learning to classify the log data.
Målet med det här projektet har varit att undersöka hur en meddelandekö kan användas för att felhantera felkoder i loggfiler mer aktivt. Projektet har följt Design Science Research Methodology för utveckling och implementering av lösningen. En modell av transaktionssystemet togs fram och emulerades i nyutvecklade applikationer. Två experiment utfördes varav det första testade en längre körning med intervall mellan meddelanden och det andra en tidmätning för hur lång tid det tar att skicka 20 000 meddelanden. Det första experimentet visade att meddelandekön klarade av att hantera meddelanden som skickades över två timmar. Det andra experimentet visade att systemet tog 14 minuter och 45 sekunder att skicka och hantera alla meddelanden, vilket gav en hög genomströmning av 22.5 meddelanden per sekund utan att några meddelanden gick förlorade. Den implementerade mottagarapplikationen tog emot alla meddelanden och lyckades räkna upp antalet felkoder som presenterades i den inkomna datan. De experiment som har utförts har bevisat att en meddelandekö kan implementeras för att felhantera felkoder i loggfiler mer aktivt. De framtida arbeten som kan utföras omfattar en utvärdering av säkerheten av systemet, jämförelser av prestanda jämfört med andra meddelandeköer, utföra experimenten på kraftfullare datorer och en implementering av maskininlärning för att klassificera loggdatan.

It is not always that easy to accurately interpret a parking signs The driver is expected to keep track of what every road sign, direction, prohibition, and amendment means, both by themselves and in combination with each others In addition, the driver must also keep track of the time, date, if there is a holiday, week number, etcs This can make the driver unsure of the rules, or interpret the rules incorrectly, which can lead to hefty fnes or even a towed vehicles By developing a mobile application that can analyze a photograph of a parking sign and quickly give the driver the verdict, the interpretation process can be made easys The purpose of this study has been to examine available technology within image and text analysis and then develop a prototype of an Android application that can interpret a photograph of a parking sign and quickly give the correct verdict, with the help of said technologys The constructed prototype will be evaluated partly by user tests to evaluate the application’s usability, and partly by functionality tests to evaluate the accuracy of the analysis processs Based on the results from the tests, a conclusion was drawn that the application gave a very informative and clear verdict, which was correct most of the time, but ran into problems with certain signs and under more demanding environmental circumstancess The tests also showed that the interface was perceived as easy to understand and use, though less interaction needed from the user was desireds There is a great potential for future development of ParCam, where the focus will be on increasing the automation of the processs
Att tolka en parkeringsskylt korrekt är inte alltid så  enkelt. Föraren förväntas ha koll på vad alla vägmärken, anvisningar, förbud, och tillägg betyder, både för sig själva och i kombination med varandra. Dessutom måste föraren även ha koll på  tid, datum, ev. helgdag, veckonummer m.m. Detta kan leda till att föraren blir osäker på vad som gäller eller tolkar reglerna felaktigt, vilket kan leda till dryga böter och även bortbogserat fordon. Genom att utveckla en mobilapplikation som kan analysera ett fotografi av en parkeringsskylt och snabbt ge svar kan denna tolkningsprocess underlättas för föraren. Syftet med denna studie har varit att utforska befintliga teknologier inom bild- och textanalys och därefter konstruera en prototyp av en Android-app som med hjälp av denna teknologi samt användarens mobilkamera kunna tolka fotografier av en parkeringsskylt och snabbt ge en korrekt utvärdering. Den konstruerade prototypen kommer att utvärderas dels genom användartester för att testa applikationens användbarhet och dels genom analys av utdata för att mäta analysens träffsäkerhet. Från testerna drogs slutsatsen att applikationen gav ett väldigt tydligt och informativt svar där analysen var korrekt de allra flesta gångerna, men stötte på problem med vissa skyltar och under svårare miljöförhållanden. Testerna visade också att gränssnittet upplevdes lätt att använda, men skulle helst kräva mindre inblandning från användaren. Det finns stor utvecklingspotential för ParCam, där fokus kommer att läggas på utökad automatisering av processen.

Applications found in both large and small enterprises need a communication method in order to meet requirements of scalability and durability. Many communication methods exist, but the most well-used are message queues and message brokers. The problem is that there exist many different types of message queues and message brokers with their own unique design and implementation choices. These choices result in different parameter sets, which can be configured in order to meet requirements of for example high durability, throughput, and availability. This thesis tests two different message brokers, Apache Kafka and RabbitMQ, with the purpose of discussing and showing the impact on throughput and latency when using a variety of parameters. The experiments conducted are focused on two primary metrics, latency and throughput, with secondary metrics such as diskand CPU-usage. The parameters chosen for both RabbitMQ and Kafka are optimized for maximized throughput and decreased latency. The experiments conducted are tested on a cloud platform; Amazon Web Services. The results show that Kafka outshines RabbitMQ regarding throughput and latency. RabbitMQ is the most efficient in terms of quantity of data being written, while on the other hand being more CPU-heavy than Kafka. Kafka performs better than RabbitMQ in terms of the amount of messages being sent and having the shortest one-way latency.
Applikationer som finns i både komplexa och icke-komplexa system behöver en kommunikationsmetod för att uppfylla kriterierna för skalbarhet och hållbarhet. Många kommunikationsmetoder existerar, men de mest använda är meddelandeköer och meddelandemäklare. Problemet är att det finns en uppsjö av olika typer av meddelandeköer och meddelandemäklare som är unika med avseende på deras design och implementering. Dessa val resulterar i olika parametersatser som kan konfigureras för att passa olika kriterier, exempelvis hög hållbarhet, genomströmning och tillgänglighet. Denna avhandling testar två olika meddelandemäklare, Apache Kafka och RabbitMQ med syfte att diskutera och visa effekterna av att använda olika parametrar. De utförda experimenten är inriktade på två primära mätvärden, latens och genomströmning, med sekundära mätvärden som exempelvis diskanvändning och CPU-användning. De parametrar som valts för både RabbitMQ och Kafka optimeras med fokus på de primära mätvärdena. Experimenten som genomförs testades på en molnplattform; Amazon Web Services. Resultaten visar att Kafka presterar bättre än RabbitMQ när det kommer till genomströmning och latens. Gällande inverkan av Kafka och RabbitMQ på mängden skriven data, är RabbitMQ den mest effektiva, medan den å andra sidan är mer CPU-tung än Kafka.

Människors levnadsstandarder förbättras ständigt tack vare nya innovativa system som möjliggörs genom att sensorer kopplas upp mot internet för att i realtid producera och analysera stora mängder viktiga data om den verkliga världens tillstånd. Luftkvalitet förvärras i världen och genom datainsamling och dataanalys kan realtidsvarningar erbjudas. Meddelandemäklare introduceras i systemarkitekturer för att samla in, lagra och strukturera datamängder på ett felsäkert sätt. Problemet är att meddelandemäklare måste kunna hantera många distribuerade luftkvalitetssensorer för att tillgodose behovet av exakt representation av luftkvalitet. Kafka och RabbitMQ sattes upp med hjälp av Docker för att under experiment undersöka vilken meddelandemäklare som tillhandahöll bäst prestanda när antalet sensorer ökade. En containeriserad webbapplikation utvecklades för att i ett gränssnitt kunna definiera exekverbara experiment. Containeriserade tjänster startades under exekvering upp. Genomsnittliga data aggregerades varje sekund till en mätpunkt för realtidspresentation i webbgränssnittet. Kafka tillhandahöll lägst latens och högst genomströmningshastighet när antalet sensorer ökade.

Det finns övrigt digitalt material (t.ex. film-, bild- eller ljudfiler) eller modeller/artefakter tillhörande examensarbetet som ska skickas till arkivet.

In this thesis, a scalable, highly available and reactive RabbitMQ cluster is implemented on Amazon Web Services (AWS) and Microsoft Azure. An alternative solution was created on AWS using the CloudFormation service. These solutions are performance tested using the RabbitMQ PerfTest tool by simulating high loads with varied parameters. The test results are used to analyze the throughput and price-performance ratio for a chosen set of instances on the respective cloud platforms. How performance changes between instance family types and cloud platforms is tested and discussed. Additional conclusions are presented regarding the general performance differences in infrastructure between AWS and Microsoft Azure.

Message-oriented middleware (MOM) is a middleware used for communication between applications. There are many different MOM technologies available today, each offering different performance (throughput and latency). The performance of MOMs depends on both message size and message guarantee settings used. The problem is that it can be difficult for users to know which MOM they should choose given their requirements. The goal was to create a performance (latency and throughput) comparison of three popular MOMs; Apache Kafka, RabbitMQ and Nats Streaming. The result shows that Kafka is the best performing MOM for smaller message sizes (under 512 bytes). RabbitMQ has the best performance for larger message sizes (over 32768 bytes). Nats Streaming only outperformed the other message system for a few combinations of message guarantee settings with the message size 4096 bytes.
Meddelandeorienterad mellanprogramvara (MOM) är mellanprogramvara som används för kommunikation mellan applikationer. Det finns många MOM system som erbjuder olika prestanda (genomströmning och latens). Prestandan är beroende av vilka meddelandegarantier som används samt meddelande storlek. Detta gör det svårt för användare att välja MOM utifrån sina krav. Målet är därför att jämföra tre populära MOMs; Apache Kafka, RabbitMQ och Nats Streaming. Resultaten visar att Kafka presterar bäst med små meddelandestorlekar (Under 512 bytes). RabbitMQ presterar bäst för större meddelanden (Över 32768 bytes) medans Nats Streaming enbart presterar bäst med ett begränsat antal meddelandegarantier och med en meddelandestorlek på 4096 bytes.

Today’s applications are required to continuously adapt and adjust, to be able to meet a constant change in demand. As result of an increasing amount of data, choosing the right communication method becomes a vital step. A solution that have been functional for a long time, may at any point in time be unable to reach the level it requires and instead turns into bottlenecks and inefficient solutions.Using a database as a communication method between system entities, does not have to be a bad solution. A database has it perks with being a simple solution and efficient query operations. However, using it as a queue management system, requires entities to continuously poll new table entries. This solution may not be the most suitable nor best available option. There exists communication system developed for the specific purpose of efficiently distributing messages to available parties.Implementing a message-oriented middleware enables for asynchronous communication which promotes applications to be more loosely coupled. As a result, available resources could be better utilised and improve the system performance. This degree project investigates the development and integration of two message-oriented middlewares, RabbitMQ and AcviteMQ, within an e-commerce system. The purpose is to explore the potentials of changing queue management system from a database to a message broker. The expected outcome is a more flexible job handling and, perhaps, an improvement of job processing by using a more efficient distribution.The results show that changing queue management system from the database to a message-oriented middleware could improve the performance of handling of invoice jobs. Testing the application servers of the Proceedo system, with a batch of invoice jobs, showed a potential of up to 17 percent faster process time using a message broker. This corresponds to a reduced process time of around 11 minutes for one application server and 6 minutes using two. Additionally, both brokers provide flexible message handling through functionality to priorities messages.
Dagens applikationer måste kontinuerligt anpassa och justera sig för att kunna möta en ständig förändring i efterfrågan. Resultat som blir av den ökande mängd data som behöver kunna hanteras, är kravet på att välja rätt kommunikationsmetod. En lösning som varit funktionell under lång tid, kan när som helst bli oförmögen att nå den nivå som krävs. Istället förvandlas den till en flaskhals och på så sätt bli en ineffektiv lösning.Att använda en databas som en kommunikationsmetod mellan systemenheter behöver inte vara en dålig lösning. En databas har förmåner som att att vara en enkel lösning och effektivt kunna hantera förfrågningar. När det appliceras som ett köhanteringssystem, krävs det att alla enheter kontinuerligt skickar nya förfrågningar för att hämta nya tabelluppdateringar. Denna lösning kanske inte är det mest lämpliga eller bästa tillgängliga alternativet. Det finns kommunikationssystem utvecklade för det här specifika syftet, att effektivt distribuera meddelanden till tillgängliga parter.Införandet av ett meddelandeorienterad middlewares gör det möjligt för asynkron kommunikation som främjar applikationer till att kunna vara mer löst kopplade. Som ett resultat kan tillgängliga resurser utnyttjas bättre och förbättra systemets prestanda. Detta examensprojekt undersöker utvecklingen och integrationen av två meddelandeorienterade middlewares, RabbitMQ och AcviteMQ, inom ett e-handelssystem. Syftet är att undersöka de positiva möjligheterna som finns av att by-ta köhanteringssystem från en databas till en meddelandeorienterad middleware. Det förväntade resultatet är en mer flexibel jobbhantering och kanske en förbättring av jobbearbetningen, genom att använda en effektivare meddelande distribution.Resultaten visar att bytet av köhanteringssystem, från databasen till en meddelandeorienterad middleware, kan förbättra hanteringen av fakturahandlingar. Testningen av Proceedo-systemets applikationsservrar visade potential på upp till 17 procent snabbare processtid med hjälp av en meddelande broker. Det motsvarar en hanteringstid på 11 minuter snabbare vid användande av en applikationserver och 6 minuter vid använding av två. Dessutom ger båda middlewares en mer flexibel meddelandehantering, i form av, funktionalitet att kunna prioritera meddelanden

Many different message-oriented middlewares (MOM) exist today on the market. With different underlying designs, purposes and features, it is not easy to make the right choice of MOM for your system. The IT company Nordicstation is in such a situation where they need to make a choice of MOM. They currently own a system called Sharelock which generates reports about violations of financial assets trad- ing. They wish to make the process of generating a report more asynchronous by using a MOM and they have a couple of requirements. This thesis was carried out with the purpose of finding the most suitable products for the system in mind, compare their features, performance, licenses and ease-of- use. This was accomplished by analysing their installation process, monitoring in- terfaces, documentation on their websites and performance in a simple throughput test. The results showed that RabbitMQ was the strongest candidate. It had good per- formance, an attractive web interface for monitoring, an easy installation and it also offered commercial support. Apache Artemis was also found to be an attractive choice but it did not have a web interface which made it hard to manage the system.
Idag finns det många meddelandebaserade mellanprogramvaror (MOM) på marknaden. Dessa har olika grundläggande designer, syften och kännetecken vilket kan göra det svårt att göra ett bra val av MOM för ett visst system. IT-företaget Nordicstation befinner sig i en sådan situation där de behöver göra ett val av MOM. De har ett system som kallas Sharelock som genererar rapporter om regelbrott in- om värdepappershandeln. De vill göra processen mer asynkron genom att använda en MOM och de har ett antal krav på produkten. Det här examensarbetet utfördes med syftet att hitta de mest passande produkter- na för det tänka systemet, jämföra deras särdrag, prestanda, licenser och anvä- ndarvänlighet. Detta gjordes genom att analysera deras installationsprocess, övervakningsgränssnitt, dokumentation på deras hemsida och prestanda i ett en- kelt prestandatest. Resultaten visade att RabbitMQ var den starkaste kandidaten. Den hade bra prestanda, ett attraktivt webbgränssnitt, en enkel installation och den erbjöd också kommersiell support åt kunder. Apache Artemis var också ett attraktivt val men den hade inget webbgränssnitt vilket gjorde det svårt att övervaka och hantera sys- temet.

Il lavoro di tesi riguarda un framework per code asincrone in Jolie sviluppato durante l'esperienza di tirocinio ad Imola Informatica. Il framework permette di modificare automaticamente la comunicazione tra due microservizi da sincrona ad asincrona, integrando il broker open-source RabbitMQ nell'architettura, e senza che lo sviluppatore debba modificare il codice dei microservizi da lui scritti. Il lavoro è stato quello di sviluppo del framework e testing dello stesso con lettura dei tempi di esecuzione e produzione di grafici.

The thesis focuses on developing prototype of e-commerce platform. This platform will be used as a base for a full-fledged e-commerce solution of specific trader in the future. The thesis deals with the analysis of the current state, analysis of available solutions, description of selected technologies, including a description of how these technologies were specifically implemented in the project. The result of this thesis is a functional prototype of e-commerce platform.

As a system grows in size, it is common that it is transformed into a microservice architecture. In order to be able monitor this new architecture there is a need to collect information from the microservices. The software company IDA Infront is transitioning their product iipax to a microservice architecture and is faced with this problem. In order to solve this, they propose the use of a Message-oriented Middleware (MOM). There exists many different MOMs that are suitable to execute this task. The aim of this thesis is to determine, in terms of latency, throughput and scalability, which MOM is best suitable for this. Out of four suitable MOMs Apache Kafka and RabbitMQ are chosen for further testing and benchmarking. The tests display that RabbitMQ is able to send single infrequent messages (latency) faster than Kafka. But it is also shown that Kafka is faster at sending a lot of messages rapidly and with an increased number of producers sending messages (throughput and scalability). However, the scalability test suggests that RabbitMQ possibly scales better with a larger amount of microservices, thus more testing is needed to get a definite conclusion.

Thesis discusses about different ways of a communication between components of a distributed system. It describes a communication using a message exchange and at the same time talks about other alternatives. It adds details about various models of a message exchange, various message types and about various specifications as well. Commercial tools ActiveMQ, RabbitMQ and Kafka are presented. Special emphasis is placed on describing the way these tools exchange messages, scalability options and others. The web service is designed according to the described features. Its main purpose is management and monitoring of the tool by user choice and easy replacement of this tool with another one. Designed application is implemented using the Kotlin language for selected tool RabbitMQ. The implemented solution allows a simple exchange of messages through the REST api.

La rapida diffusione di dispositivi connessi ad Internet e il conseguente aumento della generazione di dati ha portato le piattaforme di data processing a voler sempre di più diminuire i tempi di latenza dell'elaborazione delle informazioni. Esistono vari framework dedicati al real-time processing, tutti con vari pro e contro, dipendenti anche dal tipo di applicazione che si vuole sviluppare. In particolare, il framework RAM3S si basa su Flink, Storm e Spark, tre piattaforme di Apache con caratteristiche differenti ma tutte aderenti al paradigma dello stream processing. Tramite RAM3S lo sviluppatore viene sgravato dall'impegno di dover conoscere approfonditamente i framework prima citati, mettendo a disposizione delle interfacce per semplificare lo sviluppo delle applicazioni. In questo lavoro di tesi verranno descritti i procedimenti per includere Apache Samza e Kafka all'interno di RAM3S. Samza è un framework per lo stream processing da affiancare a Flink, Storm e Spark mentre Kafka mantiene la coda di messaggi che, al momento, viene amministrata da RabbitMQ. Verranno inoltre svolte alcune analisi di prestazioni per valutare velocità e throughput del sistema in seguito ai cambiamenti prima citati.

Att flera datorer används för att gemensamt lösa problem är inte någonting nytt. Det finns många distribuerade system i bruk och många olika lösningar för hur dessa ska kommunicera med varandra. Vissa använder sig av meddelande-orienterade lager för kommunikation vilket det finns väldigt många implementationer av. RabbitMQ är ett exempel där att kommunikation går genom en (eller ett kluster av) central nod och kommunicerar med hjälp av protokollet Advanced Message Queue Protocol, AMQP. I en helt annan kategori finns ZeroMQ som inte definierar någon central nod för all kommunikation att passera utan peer to peer är istället möjlig vilket innebär snabbare responstider men försvårar hur olika klienter hittar till varandra. Det bloggindexerande företaget Twingly kör idag med ett distribuerat system som använder flera olika kösystem för att koordinera ut arbete till de olika datorerna. De vill kolla närmare på hur de kan bygga sitt system med hjälp av meddelande-orienterade lager. Resultatet av arbetet är att RabbitMQ innebär mindre komponenter att hålla reda på vilket innebär att koden blir mindre komplex. Det som kommer gratis med att använda RabbitMQ är just att klienterna inte behöver känna till varandra utan endast behöver känna till RabbitMQ-servern. Nackdelen är att RabbitMQ-servern kommer bli en flaskhals för systemet. ZeroMQ är däremot friare att implementera den funktionalitet man själv behöver vilket är till fördel i de fall tid och pengar finns för att skapa ett eget system byggt ovanpå ZeroMQ. För Twingly som vill ha ett system inom en snar framtid är RabbitMQ ett bättre val av dessa två alternativ.

L’elaborazione di quantità di dati sempre crescente ed in tempi ragionevoli è una delle principali sfide tecnologiche del momento. La difficoltà non risiede esclusivamente nel disporre di motori di elaborazione efficienti e in grado di eseguire la computazione coordinata su un’enorme mole di dati, ma anche nel fornire agli sviluppatori di tali applicazioni strumenti di sviluppo che risultino intuitivi nell’utilizzo e facili nella messa in opera, con lo scopo di ridurre il tempo necessario a realizzare concretamente un’idea di applicazione e abbassare le barriere all’ingresso degli strumenti software disponibili. Questo lavoro di tesi prende in esame il progetto RAM3S, il cui intento è quello di semplificare la realizzazione di applicazioni di elaborazione dati basate su piattaforme di Stream Processing quali Spark, Storm, Flinke e Samza, e si occupa di esaudire il suo scopo originale fornendo un framework astratto ed estensibile per la definizione di applicazioni di stream processing, capaci di eseguire indistintamente sulle piattaforme disponibili sul mercato.

Genetic algorithms are modern algorithms intended to solve optimization problems. Inspiration originates in evolutionary principles in nature. Parallelization of genetic algorithms provides not only faster processing but also new and better solutions. Parallel genetic algorithms are also closer to real nature than their sequential counterparts. This paper describes the most used models of parallelization of genetic algorithms. Moreover, it provides the design and implementation in programming language Python. Finally, the implementation is verified in several test cases.

There is a need to develop a range different VLP for use as nanoscale templates and vaccines. The aim of this research was to develop RHDV VLP as a versatile vaccine delivery system easily modified for use against a wide range of different diseases. Production of Rabbit haemorrhagic disease virus (RHDV) capsid protein in a baculovirus system led to the self-assembly of Virus-like Particles (VLP) that could be purified to greater than 99% purity using simple methods. The capsid gene, vp60, can be manipulated genetically to incorporate immunogenic peptide sequences or a functional DNA-binding site. Fusion of these small epitopes to VP60 was well tolerated, forming VLP and greatly enhanced the presentation of peptide to, and activation of CD4+ T helper cell hybridoma. To avoid constraints imposed on chimeric VLP and dramatically increase the versatility of RHDV VLP, rapid conjugation of antigen was carried out, employing the hetero-bifunctional chemical linker, sulpho-SMCC. Incorporation of sulfhydral groups by design or treatment with SATA allowed for great versatility, in turn enabling many diverse peptides and proteins to be conjugated to VLP. RHDV VLP and consequently the conjugated GFP antigen were efficiently taken up by DC with more than 85% of DC positive for GFP by flow cytometry. This was also visualised by confocal microscopy and electron microscopy of both gold- labelled VLP and conjugated antigen. RHDV VLP conjugate was shown to induce the significant up regulation of the activation markers CD40, CD80, CD86 and MHC class II on the surface of dendritic cells (DC). As well, DC pulsed with RHDV VLP/OVA effectively presented OVA to both CD4+ and CD8+ T cells transgenic for respective peptide-specific T cell receptors, eliciting a greater proliferative response in both T cell subsets than antigen delivered alone. The surface accessibility of peptides on VLP was demonstrated, while administration of VLP/Ovalbumin (OVA) conjugate in mice was shown to evoke very high titre antibody responses specific for conjugated antigen. VLP/OVA conjugates were also shown to induce IFN-γ production and OVA-specific cytotoxic killing in vivo, of up to 80% of fluorescently labelled, adoptively transferred target cells. No distinguishable cytotoxicity was detected in unimmunised control mice. This assay was also used to demonstrate the necessity for antigen to be conjugated to VLP, as antigen mixed with VLP induced only sub-optimal killing. To investigate the anti-tumour effects, mice vaccinated with VLP conjugated to OVA protein, CD4+ or CD8+ T cell OVA epitopes were inoculated with B16- OVA tumour cells and monitored for tumour growth. Untreated control mice had to be sacrificed by day 19, while mice immunised with either VLP/OVA or VLP conjugated with both CD4+ and CD8+ OVA epitopes, showed a significant delay in tumour growth (P = 0.0002), with one mouse remaining free of palpable tumour until day 92. These results show that RHDV VLP can be easily produced and purified and demonstrate the versatility of this RHDV capsid. Rapid conjugation techniques allowed the modification of VLP with both peptide and protein rendered these antigens highly immunogenic, stimulating both humoral and cell-mediated immunity targeted against conjugated antigens of choice. The versatility and immune stimulating properties of RHDV VLP provides a molecular tool with almost limitless applications within the fields of nanotechnology and immunology.

With the substantial growth of cloud computing over the past decade, microservices has gained significant popularity in the industry as a new architectural pattern. It promises a cloud-native architecture that breaks large applications into a collection of small, independent, and distributed packages. Since microservices-based applications are distributed, one of the key challenges when designing an application is the choice of mechanism by which services communicate with each other. There are several approaches for implementing Interprocess communication (IPC) in microservices, and each comes with different advantages and trade-offs. While theoretical and informal comparison exists between them, this thesis has taken an experimental approach to compare and contrast common forms of IPC communications. In this the- sis, IPC methods have been categorized into Synchronous and Asynchronous categories. The Synchronous type consists of REST API and Google gRPC, while the Asynchronous type is using a message broker known as RabbitMQ. Further, a collection of microservices for an e-commerce scenario has been designed and developed using all the three IPC methods. A load test has been executed against each model to obtain quantitative data related to Performance Efficiency, and Availability of every method. Developing the same set of functionalities using different IPC methods has offered a qualitative data related to Scalability, and Complexity of each IPC model. The evaluation of the experiment indicates that, although there is no universal IPC solution that can be applied in all cases, Asynchronous IPC patterns shall be the preferred option when designing the system. Nevertheless, the findings of this work also suggest there exist scenarios where Synchronous patterns can be more suitable.
Med den kraftiga tillväxten av molntjänster under det senaste decenniet har mikrotjänster fått en betydande popularitet i branschen som ett nytt arkitektoniskt mönster. Det erbjuder en moln-baserad arkitektur som delar stora applikationer i en samling små, oberoende och distribuerade paket. Eftersom microservicebaserade applikationer distribueras och körs på olika maskiner, är en av de viktigaste utmaningarna när man utformar en applikation valet av mekanism med vilken tjänster kommunicerar med varandra. Det finns flera metoder för att implementera Interprocess-kommunikation (IPC) i mikrotjänster och var och en har olika fördelar och nackdelar. Medan det finns teoretisk och in- formell jämförelse mellan dem, har denna avhandling tagit ett experimentellt synsätt för att jämföra och kontrastera vanliga former av IPC-kommunikation. I denna avhandling har IPC-metoder kategoriserats i synkrona och asynkrona kategorier. Den synkrona typen består av REST API och Google gRPC, medan asynkron typ använder en meddelandemäklare känd som RabbitMQ. Dessutom har en samling mikroservice för ett e-handelsscenario utformats och utvecklats med alla de tre olika IPC-metoderna. Ett lasttest har utförts mot var- je modell för att erhålla kvantitativa data relaterade till prestandaeffektivitet, och tillgänglighet för varje metod. Att utveckla samma uppsättning funktionaliteter med olika IPC-metoder har erbjudit en kvalitativ data relaterad till skalbarhet och komplexitet för varje IPC-modell. Utvärderingen av experimentet indikerar att även om det inte finns någon universell IPC-lösning som kan tillämpas i alla fall, ska asynkrona IPC-mönster vara det föredragna alternativet vid utformningen av systemet. Ändå tyder resultaten från detta arbete också på att det finns scenarier där synkrona mönster är mer lämpliga.

La tesi è volta a presentare quanto svolto presso l'azienda FastCode Consulting Srl di Cesena. Il lavoro ha compreso la riprogettazione dell'applicazione aziendale abbandonando la struttura monolitica e adottando l'architettura a microservizi. Successivamente si è dato il via allo sviluppo di tale riprogettazione con l'implementazione di un microservizio che consente di gestire i corsi aziendali e la formazione del personale.

I sistemi software nati dall'esigenza di supportare agevolmente ed efficacemente il lavoro cooperativo, in particolare quelli orientati al supporto di azioni di soccorso in scenari di emergenza, appaiono tutt'ora fortemente limitati e frammentati. In molti casi vengono affrontate solamente specifiche dimensioni del problema complessivo, anche se il livello al quale è giunto lo sviluppo tecnologico e i risultati osservati in ambito di ricerca permettono di delineare soluzioni complete e significative per l'impiego in ambiti reali. Tale tipologia di sistemi è stata scelta per il grande interesse che desta sia dal punto di vista accademico, essendo costituita da molteplici sotto--sistemi spesso eterogenei che debbono necessariamente interagire e supportare l'azione umana, sia dal punto di vista industriale, interpretando la necessità crescente di iniettare nel maggior numero possibile di livelli sociali la forte dipendenza (il supporto allo stesso tempo) dalle scienze tecnologiche ed informatiche, per rafforzare e talvolta estendere le possibilità dell'essere umano in quanto tale. Dopo una prima fase in cui verrà delineato un quadro concettuale piuttosto dettagliato circa i principali elementi e problematiche che caratterizzano la classe di sistemi considerati, sarà dato spazio alla validazione di tali principi e considerazioni emerse, confrontandosi con la progettazione e sviluppo in forma prototipale di un sotto--sistema relativo ad un caso di studio reale, significativo per l'ambito di applicazione, nato dalla collaborazione con l'Università degli Studi di Bologna di un'azienda della regione Emilia--Romagna. Il sistema software realizzato vuole essere innanzi tutto la risposta alle esigenze emerse nel caso di studio trattato, in modo tale da potersi sostituire agli attuali limitati supporti alla cooperazione, ma anche un esperimento che possa essere considerato un artefatto centrale da utilizzare come base di conoscenza condivisa, in cui vengano fattorizzati i concetti e meccanismi chiave, fondamentali per sviluppi futuri.

Mestrado em Engenharia de Computadores e Telemática
Com conceitos como a internet das coisas a surgir e a tornarem-se cada vez mais populares, criar ligações entre veículos foi apenas um próximo passo lógico, formando assim as redes ad hoc veiculares. Estas redes são um caso particular das redes móveis ad hoc, nas quais os veículos se ligam uns aos outros de uma forma espontânea. Acrescentar aos veículos a capacidade de comunicarem uns com os outros faz surgir uma abundância de possibilidades. Contudo, atualmente já existem diversas aplicações que fazem uso destas redes; no entanto, a maioria destas aplicações estão mais diretamente relacionadas com a ccomunicação entre veículo e não entre utilizadores. Soluções como o REINVENT fornecem a capacidade de expedir mensagens através de uma VANET utilizando smartphones, contudo falta-lhe uma camada lógica capaz de suportar a expedição de mensagens de utilizador para utilizador. A nossa contribuição, o Sistema de Mensagens para Redes Veiculares (VNMS), permite a troca de mensagens entre utilizadores numa VANET. Com a implantação de um quadro de avisos virtual nos nós da VANET, com uma camada de reencaminhamento de mensagens e um naming service, fornece aos utilizadores a capacidade de trocarem mensagens entre si sem a necessidade de informação ou serviços da VANET. Os nós do VNMS atuam como agregadores de mensagens, providenciando repositórios locais de mensagens de utilizadores e reencaminhamento sobre a rede para o utilizador alvo, i.e., o nó ao qual o utilizador está ligado. Na perspetiva do utilizador, este pode usar os serviços do VNMS de uma forma transparente através de uma aplicação Android – foi criada uma aplicação de chat que usa a VANET como prova de conceito.
With concepts like the internet of things currently cropping up and getting more popular, connecting vehicles with each other was just a logical step, originating the vehicular ad-hoc networks (VANETs). VANETs are a particular case of Mobile ad-hoc networks (MANETs) in which vehicles connect with other vehicles in ad-hoc mode and evolving topologies. By enhancing vehicles with the ability to communicate with each other, an abundance of capabilities arises. However, currently most applications using VANETs are focused on the vehicle to vehicle communications, and not on vehicles users, either drivers or passengers. Previous work like REINVENT provided a solution capable of dispatching messages through VANETs using standard smartphones; however, it lacked a logical layer to support user to user logical message brokering. Our contribution, the Vehicular Network Messaging System (VNMS), allows user to user message exchange on VANET. By deploying virtual bulletin boards (VBBs) in VANETs nodes, a layer of message forwarding, and user naming service, it provides users the ability to exchange messages without the explicit need of any VANETs specific information or service. VNMS nodes act as brokers for user messages, providing local user message repositories and VANETs routing to targeted user(s) i.e. its VANET node. From the user perspective, it is possible to use VNMS services transparently using Android mobile application – we implemented a VANETs enabled chat application as proof of concept.

The common ringtail possum (Pseudocheirus peregrinus), a member of the family Pseudocheiridae, is an arboreal folivorous marsupial that feeds predominantly on Eucalyptus foliage. Contrary to the expectation that small body size would inhibit utilisation of a diet containing such high levels of lignified fibre because of relatively low gut volume to body mass ratios and relatively high mass-specific metabolic rates and nutrient requirements (Hume 1999), the ringtail possum is able to survive solely on a diet of Eucalyptus foliage. The rabbit (Oryctolagus cuniculus) is a terrestrial herbivore and is a member of the family Leporidae that feeds predominantly on grasses. The rabbit was proposed as a digesta flow model for the ringtail possum since both are caecotrophic (periodically re-ingest caecal contents) and both are proposed to exhibit a colonic separation mechanism (CSM) where fluids and small, easily digested particles are preferentially returned to the caecum. The rabbit is of value for the modelling process since it is more accessible for experimental manipulation than the ringtail possum. This study investigated a proposal to use digesta passage through the gastrointestinal tract (GIT) of the rabbit as a model of digesta passage for the ringtail possum on the basis that both are caecotrophic caecum fermenters. A number of potential problems were identified with this proposal and investigation of these problems formed the basis for the research described in this thesis. Two main areas were identified as being potentially problematic: 1) fundamental flaws with the particulate markers used in digesta rate of passage studies; and 2) differences in animal behaviour and natural diet between the two subject species which suggested different digestive strategies and hence different patterns of digesta flow through the GIT. The proposed digesta passage markers were lanthanide metals (Dy, Tm, Eu and Yb) attached to either fibrous particles (1200 - 600�m) or formalin-fixed rumen bacteria (20 � 0.2�m). These markers were shown to not be of the assumed size classes and the extent of lanthanide metal binding differed between the four metals used. An effect due to method of dosing was also observed. The findings of marker inconsistencies caused major limitation to model development and further research is necessary to clarify these markers. The proposal to use digesta flow in the rabbit GIT as a model for digesta flow in the ringtail possum was shown to be idealistic due to the differences in anatomy and behaviour observed between the two herbivores. Laboratory observations, time series analysis and compartmental modelling confirmed the differences between the animals. This study showed: 1) the GIT of the rabbit was more complex both anatomically and functionally than that of the ringtail possum; 2) behaviour affecting digesta passage of the rabbit and ringtail were different and; 3) compartmental models confirmed the anatomical and behavioural findings. Digesta passage in the rabbit could not be modelled mathematically using data on digesta passage due to complexities of the system. In contrast, a basic model was constructed for digesta passage in the ringtail possum. On the basis of these findings, the research hypothesis "that digesta passage in rabbits is similar to that in ringtail possums" was rejected.

The European rabbit lives in defined social groups of between two and about twenty individuals. There are distinct social hierarchies within each group. Rabbits are known to scent-mark their environment with secretions from several glands, and the secretion of the submandibular cutaneous gland is strongly correlated with social status. Dominant, male rabbits have a higher secretory activity of the gland, and show much more scent-marking behaviour than do any other individuals within the social group. This study was principally conducted at Hope Farm, Cattai National Park in New South Wales, Australia.The proteinaceous components of the secretion varied between individuals, but the protein profile of an individual did not change over time.It was found that dominant rabbits chin mark preferentially at the entrances to warrens, and at the boundaries of their territory.The work in this study provides new insights into the way that rabbit semiochemical messages work. The difference between the secretion of a subordinate and a dominant rabbit appears to be due to the presence or absence of one compound, 2-phenoxy ethanol. This compound, with known fixative properties, supports the idea that the only difference between dominant and subordinate secretions is whether or not they persist in the environment after marking. Such a mechanism for asserting dominance may be much more common in mammals than is apparent from the published literature
Doctor of Philosophy (PhD)

[ES] Cualquier mejora en el conocimiento de la nutrición y el manejo de los futuros conejos reproductores debe considerarse como prioritario en la producción cunícola. Sin embargo, los principales esfuerzos en investigación se han centrado en estos animales durante la fase de reproducción propiamente dicha. La hipótesis inicial de esta tesis fue que alcanzar un desarrollo temprano adecuado durante la gestación, lactancia, engorde y recría, con la ayuda de programas ajustados de alimentación en algunos de estos períodos, mejoraría el rendimiento reproductivo y la vida útil de los futuros conejos reproductores. Para evaluar esta hipótesis, se llevaron a cabo dos tipos de ensayos, tanto en conejos hembra de líneas maternas como en machos de líneas seleccionadas por velocidad de crecimiento y destinadas a la inseminación artificial. El primero de estos ensayos se centró en los diferentes patrones de crecimiento, desde el nacimiento hasta el final de la fase de recría, y su influencia en la carrera reproductiva y la vida útil del conejo. En el segundo tipo de pruebas, se evaluó el efecto del uso de diferentes dietas o programas de alimentación durante el período de recría en la productividad a corto y medio plazo. Los resultados muestran dos fases importantes en el desarrollo temprano, de acuerdo con sus efectos sobre el rendimiento reproductivo y la vida útil. Mostrar un mayor desarrollo corporal desde el nacimiento hasta los 63 días de vida parece tener una influencia positiva en el grado de madurez y la productividad al comienzo de su vida reproductiva, además de poder mejorar su supervivencia a corto plazo, pero con posibles efectos negativos en la esperanza de vida a largo plazo. Sin embargo, el desarrollo durante el período de recría (desde los 63 días de vida hasta el comienzo de la carrera reproductiva) parece tener un mayor impacto en la productividad y la esperanza de vida de nuestros futuros conejos reproductores. Las hembras más engrasadas en la primera inseminación tuvieron tamaños de camada más pequeños y un mayor riesgo de ser sacrificadas que las magras (P<0.05). Además, los machos que mostraron una mayor ganancia media diaria durante el período de recría tuvieron un número y porcentaje de eyaculados viables más bajos a lo largo de su vida productiva y mayor riesgo de muerte o sacrificio. Estos resultados podrían indicar que la clave para llevar a cabo un desarrollo adecuado durante el período de recría debería basarse tanto en conseguir que los conejos alcancen un grado de madurez apropiado, tanto fisiológica como reproductivamente, así como evitar un excesivo engrasamiento al inicio de su vida productiva. En los ensayos destinados a evaluar los programas de alimentación durante la recría, el uso de una restricción alimentaria en hembras, y especialmente el uso de dietas fibrosas (de 8.5 a 10 MJ ED/kg MS) permiten un desarrollo corporal gradual y adecuado a la edad de cubrición, con suficientes reservas corporales para afrontar el comienzo de la vida reproductiva y mejorando tanto su rendimiento reproductivo como su vida útil. El éxito de estos programas de alimentación de recría depende del momento y la duración de la aplicación de estos, mejorándose con la aplicación de un cambio a un pienso enriquecido energéticamente alrededor del primer apareamiento. En los machos, se observó que un programa de alimentación durante la recría adecuado debería adaptarse a la capacidad de ingesta de estos y a sus necesidades nutricionales en períodos concretos de la recría, consiguiendo así mejoras en algunos parámetros del semen al inicio de su vida reproductiva. En conclusión, tanto un desarrollo temprano adecuado desde la gestación hasta el comienzo de su vida reproductiva, como el uso de programas adecuados de alimentación durante la recría podrían mejorar significativamente el rendimiento reproductivo futuro, así como la esperanza de vida, tanto en conejos he
[CAT] Qualsevol millora en el coneixement tant de la nutrició com del maneig dels futurs conills de criança ha de considerar-se com a prioritari en la producció de conills. No obstant açò, els principals esforços en investigació s'han centrat en aquests animals durant la fase de reproducció pròpiament dita. La hipòtesi inicial d'aquesta tesi va ser que aconseguir un desenvolupament primerenc adequat durant la gestació, lactància, engreix i criança, amb l'ajuda de programes ajustats d'alimentació en alguns d'aquests períodes, milloraria el rendiment reproductiu i la vida útil dels futurs conills reproductors. Per a avaluar aquesta hipòtesi, es van dur a terme dos tipus d'assajos, tant en conills femella de línies maternes, com en mascles de línies seleccionades per a la velocitat de creixement i destinades a la inseminació artificial. El primer d'aquests assajos es va centrar en els diferents patrons de creixement, des del naixement fins al final de la fase de criança, i la seua influència en la carrera reproductiva i la vida útil del conill. En el segon tipus de proves, es va avaluar l'efecte de l'ús de diferents dietes o programes d'alimentació durant el període de criança, en la productivitat a curt i mig termini. Els resultats obtinguts semblen indicar que podríem diferenciar dues fases en el desenvolupament primerenc, d'acord amb els seus efectes sobre el rendiment reproductiu i la vida útil. Mostrar un major desenvolupament corporal des del naixement fins als 63 dies de vida sembla tenir una influència positiva en el grau de maduresa i la productivitat al començament de la seua vida reproductiva, a més de poder millorar la seua supervivència a curt termini, però amb possibles efectes negatius en l'esperança de vida a llarg termini. No obstant açò, el desenvolupament durant el període de sembla tenir un major impacte en la productivitat i l'esperança de vida dels nostres futurs conills reproductors. Les femelles més greixades en la primera inseminació van tenir grandàries de ventrada més xicotets (tant nascuts com deslletats) i un major risc de ser sacrificades que les magres (P<0.05). A més, els mascles que van mostrar un major guany mitjà diari durant el període de criança van tenir un nombre i percentatge d'ejaculats viables més baixos al llarg de la seua vida productiva i major risc de mort o sacrifici. Aquests resultats podrien indicar que la clau per a dur a terme un desenvolupament adequat durant el període de criança hauria de basar-se tant a aconseguir que els conills aconseguisquen un grau de maduresa apropiat, per a afrontar els futurs desafiaments productius, com evitar un excessiu greixatge a l'inici de la seua vida productiva. En els assajos destinats a avaluar els programes d'alimentació durant la criança, l'ús d'una restricció alimentària en femelles, i especialment l'ús de dietes fibroses (de 8.5 a 10 MJ ED/kg MS) permeten un desenvolupament corporal gradual i adequat a l'edat de cobriment, amb suficients reserves corporals per a afrontar el començament de la vida reproductiva i millorant tant el seu rendiment reproductiu com la seua vida útil. L'èxit d'aquests programes d'alimentació de criança depèn del moment i la durada de l'aplicació d'aquests, millorant-se amb l'aplicació d'un canvi a un pinso enriquit energèticament al voltant del primer cobriment. En els mascles, es va observar que un programa d'alimentació durant la criança adequat hauria d'adaptar-se a la capacitat d'ingesta d'aquests i a les seues necessitats nutricionals en períodes concrets de la criança, aconseguint així millores en alguns paràmetres del semen a l'inici de la seua vida reproductiva. En conclusió, tant un desenvolupament primerenc adequat des de la gestació fins al començament de la seua vida reproductiva, com l'ús de programes adequats d'alimentació durant la criança podrien millorar significativament el rendiment reproductiu futur, així com l'esperança
[EN] Any improvement on the knowledge of both nutrition and management of the future rabbit breeders should be considered as crucial. However, main research efforts have been focused on animals during reproduction phase. The initial hypothesis of this thesis was that achieving an adequate early development during young rabbits' gestation, lactation, fattening and rearing, with the help of fitted feeding programmes in some of these periods, would be improved reproductive performance and lifespan of the future breeding rabbits. To evaluate this hypothesis, two types of trials, both in rabbit females from maternal lines selected for litter size at weaning and males from paternal lines selected for growth rate and intended on artificial insemination, were carried out. The first of these trials were mainly focused on the different growth patterns, from birth to the end of the rearing age, and their influence on rabbit's reproductive career and lifespan. In a second type of trials, the effect of the use of different diets or feeding programs during the rearing period in the performance in the short- medium-term was evaluated. The results obtained from the evaluation of the different growth patterns trials seem to indicate that, we could differentiate two parts on the early development according to their effects on reproductive performance and lifespan. To show a greater body development from birth to 63 days of life seems to have a positive influence on the maturity degree and productivity at the beginning of their reproductive life, as well as being able to improve their survival in the short-term, but it could be even negative for long-term lifespan. However, the development during the rearing period (from 63 days of life to the beginning of reproduction career) seems to have a higher impact on the productivity and lifespan of our rabbit breeders. Fatter rabbit females at first insemination had smaller litter sizes (born and weaned) and a higher risk of being culled (+13% per positive change in mm of perirenal fat) than lean ones (P<0.05). Also, rabbit males that showed a greater average daily gain during rearing period had a lower number and percentage of profitable ejaculates through it productive life (-10.1 and -4.9% per each 10 g of daily gain increase, respectively; P<0.05) and higher risk of death or culling (+16.2% per each increase in one standard deviation unit on live weight; P<0.05). These results could indicate that the key to carrying out an adequate development during the rearing period should allow the rabbits to reach an appropriate degree of physiological and reproductive maturity to face the future productive challenges, avoiding an excessive fatness at the beginning of their productive life. The use of a feeding restriction in rabbit females, and especially the use of fibrous diets (from 8.5 to 10 MJ DE/ kg DM) lead a gradual and adequate body development at mating, with enough body reserves to face the beginning of reproduction life and improving their whole reproductive performance and lifespan. The success of these rearing feeding programs depends in the moment and duration of application of them and improved with the application of a nutritional flushing around first mating. In rabbit males, it was observed that an adequate rearing feeding program should adapt it to the male's intake capacity and their nutritional requirements rearing period, especially to their high DP needs at the beginning of rearing period or when their requirements were close to maintenance, to improve some semen parameters at the beginning of their reproduction life. In conclusion, both the adequate early development of future rabbit breeders, from their own gestation to the beginning of their reproductive life, and the use of appropriate rearing feeding programs could significantly improve their future reproductive performance, as well as their life expectancy, both in rabbit females and males.
Martínez Paredes, EM. (2019). Rearing programmes for breeding rabbits. Effect of early development and feeding on future reproductive performance of rabbit females and males [Tesis doctoral no publicada]. Universitat Politècnica de València. https://doi.org/10.4995/Thesis/10251/124969
TESIS

Thesis (Ph.D.) -- University of Western Sydney, Hawkesbury, 2000.
A thesis presented to the University of Western Sydney, Hawkesbury, in partial fulfilment of the requirements for the degree of Doctor of Philosophy, December, 2000. Includes bibliographical references.

The experiments described in this thesis were designed to assess changes in pasture dynamics (biomass and species composition of pasture) of grazing land on the Southern Tablelands of ACT and NSW, after 16 combinations of rabbit control treatments had been applied. The rabbit control performed by CSIRO Division of Wildlife and Ecology consisted of all combinations of presence-absence of Poisoning (using sodium monofluoroacetate, 1080: POIS), Ripping (ripping warrens using a tractor fitted with ripping tynes: RIP), Fumigation (pressure fumigation using chloropicrin: FUM) and repeated follow-up fumigation (using phostoxin pellets one, six and eighteen months after completion of the initial treatment: ANN). The pasture was assessed before treatments were applied, and every six months after rabbit control treatments. Treatment combinations were assigned randomly in a 24 factorial design on a total of 32 sites. There was a significant increase of pasture biomass at the RIP+ANN treatment at post-treatment sample 5. The analysis of covariance did not detect any other significant increase or decrease of pasture biomass for any rabbit control treatment, at any posttreatment sample. A significant increase of grass species occurred for the treatments of POIS+RIP+FUM, POIS and RIP+ANN for the post-treatment samples of 1, 3 and 5 respectively. There was a significant increase of thistles at the rabbit control treatments of POIS+RIP+FUM+ANN (post-treatment sample 1), RIP, ANN, RIP+FUM, RIP+FUM+ANN and POIS+RIP+FUM+ANN (post-treatment sample 3) and RIP and FUM+ANN (post-treatment sample 5). A significant increase of weeds occurred at FUM (post-treatment sample 3) and at FUM+ANN (post-treatment sample 5). No significant changes in the amount of herbs or legumes was apparent for any rabbit control treatment or post-treatment sample. There were no significant decreases for any species group. Except for the significant results for post-treatment sample 1, all significant increases of biomass for any species group occurred during spring (post-treatment sample 3 and 5) which suggests a growth phase during spring then subsequent dieback (particularly for thistles and weeds), as any change was not detected in the following autumn sample. No strong trend is evident for any particular rabbit control treatments, or any combination of treatments. Analysis of covariance revealed that the rabbit control treatment of RIP+ANN showed significant increases in both total biomass of pasture and grass biomass during post-treatment sample 5. This treatment reduced the number of active entrances the most. Significant positive correlations were found between pasture biomass (total) with grass, herb, legume, thistle and weed species groups. Significant negative correlations between grass biomass and the number of active entrances were found when the rabbit control had been highly effective in reducing the number of active entrances. When rabbit control had not been very successful, there was a significant positive but low correlation with the number of active entrances. There was no significant relationship between the number of active entrances with the weight of rabbit dung pellets. It is reasoned that they are different measures of rabbit abundance. More rabbit dung pellets were found closer to the warren than further away from the warren, but there was no correlation between rabbit dung and pasture biomass. Rainfall was above average for most of the experiment, biomass increased accordingly, and rabbit control was highly successful. The resulting changes in the pasture were difficult to detect, although some increases in species composition groups occurred. It is reasoned that the changes observed are partly attributable to seasonal conditions, and to high rainfall. Grazing by domestic animals, sheep and cattle, had been found to be consistent throughout the experiment.

Two populations of rabbits (Oryctolaqus cuniculus) were investigated to see whether polygynous, multi-male groups formed in the absence of large multi-entranced warrens. They did not. Rabbits neither gathered in space nor time. The small warrens were spread out evenly across homogeneous patches and the females were well spaced out. Monogamy, distinguished by a battery of tests, was prevalent, with the more dominant males as 'mate' rabbits. That the polygyny frequently mentioned in the literature was a result of male dominance and female defense was considered. The genetic structure of each population was investigated by taking blood from rabbits and having it analysed electrophoretically and for immunoglobulins. A method for assessing relatedness between groups of pairs of animals was implemented, then validated and developed with Monte Carlo simulations. With the seven polymorphic allele obtained, no non-zero relatedness was found but it was sometimes possible to exclude high relatedness. The bearing of sociality on vigilance during feeding was investigated. Although a rabbit's vigilance decreased as its 'mate' approached, the presence of other rabbits was correlated with increased vigilance. It was concluded that the need for social vigilance outweighed the benefit of 'many eyes' watching for predators. This conclusion was tested by experiment, using stuffed animals as stimuli. Rabbits increased their vigilance during grazing bouts both by increasing the length and frequency of scans. Scans could be short or long: the probability of ending a scan decreased sharply at a certain point; a form of positive feedback. The durations of short 'maintenance' scans were dependent on chewlength (the amount of food in the mouth). This fitted a timesharing definition as supported by experiment. Long scans in response to a visible threat did not involve chewing.

Olfactory behaviour in the European rabbit (Oryctolagus cuniculus) has been studied extensively under laboratory and semi-natural conditions. Results of observations on aspects of the olfactory behaviour of a free-living population of rabbits are presented. To facilitate interpretation of these results, considerable preliminary information about the population was collected. The study was conducted over a three year period on the Isle of May off the east coast of Scotland. A total of 326 rabbits were trapped and marked to permit identification in the field, and data on sex, age and social status of these individuals was collated. Data on the overall structure and fluctuations in the population are presented. The social organisation and home ranges of rabbits at four study sites throughout the three years are described. Observations indicate that the social organisation of free-living populations is more complex and variable than previous descriptions of semi-natural populations would have led us to expect. The reproductive performance of the population was investigated and intra and interwarren variations are analysed with respect to warren and group size. Results indicate an inverse relationship between warren size and reproductive success. The most frequently observed group composition (2 males, 2 females) was also the most reproductively successful. Daily and seasonal activity patterns of different age, sex and social status classes of rabbits are described. Olfactory communication was investigated by analysis of the frequency, daily and seasonal variation, and behavioural context of odour related activities performed by members of different age, sex and social status classes. The importance of using appropriate methods for the sampling of behaviour in field studies of olfaction is stressed. The present study concentrates on behaviour related to latrines; chin marking of the substrate and of conspecifics; enurination and urine squirting; and pawscraping. The results suggest that different scent products may carry similar information but analysis of variations in the frequency and context of odour deposition suggests that the deposition of scent fulfills a variety of functions.

Thesis (Ph. D.)--University of Nevada, Reno, 2007.
"May, 2007." Includes bibliographical references. Online version available on the World Wide Web. Library also has microfilm. Ann Arbor, Mich. : ProQuest Information and Learning Company, [2007]. 1 microfilm reel ; 35 mm.

El objetivo de esta tesis ha sido estudiar la respuesta directa a la selección por tasa de ovulación en conejo y las respuestas correlacionadas en tamaño de camada y tasas de supervivencia. Los animales pertenecían a una línea de conejos seleccionada por tasa de ovulación durante 10 generaciones. La selección se realizó en base al valor fenotípico de la hembra, que se midió el día 12 de la segunda gestación mediante laparoscopia. Se creó una línea control a partir de la recuperación de aproximadamente 470 embriones de 50 hembras donantes de la generación base. Los embriones fueron vitrificados y almacenados en nitrógeno líquido hasta su transferencia al final del experimento de selección (generación 10 de la línea seleccionada). Se midieron los siguientes caracteres: tamaño de camada (LS), estimada como el número total de gazapos al parto en un máximo de 5 partos; tasa de ovulación (OR), estimada como el número de cuerpos lúteos en los dos ovarios; tasa de ovulación derecha y tasa de ovulación izquierda (ROR y LOR); el número de embriones implantados totales (IE), en el lado derecho (RIE) y en el lado izquierdo (LIE); la diferencia ovulatoria (OD), definida como la diferencia entre ROR y LOR, expresada en valor absoluto; la diferencia de implantación (ID), definida como la diferencia entre RIE y LIE, expresada en valor absoluto; la supervivencia embrionaria (ES), calculada como IE/OR; la supervivencia fetal (FS), calculada como LS/IE; la supervivencia prenatal (PS), calculada como LS/OR. Se utilizó metodología bayesiana para analizar los datos. Las estimas de las heredabilidades de OR, LS, ES, FS y PS fueron 0.16, 0.09, 0.09, 0.24 y 0.14, respectivamente. Las estimas de las correlaciones fenotípicas de OR con LS, ES, FS y PS fueron 0.09, -0.07, -0.26 and -0.28, respectivamente. Las estimas de las correlaciones genéticas de OR con LS y ES tuvieron una baja precisión, y no se pudo concretar su signo. Las estimas de las correlaciones genéticas de OR con FS y PS fueron negativas (probabilidad de ser negativa de 1.00 y 0.98, respectivamente). Las correlaciones fenotípicas y genéticas entre LS y las tasas de supervivencias fueron positivas (probabilidad de ser positivas de 1.00).
Laborda Vidal, P. (2011). Selection for ovulation rate in rabbits [Tesis doctoral no publicada]. Universitat Politècnica de València. https://doi.org/10.4995/Thesis/10251/14121
Palancia

Dissertação (Mestrado) - Universidade Federal de Santa Catarina, Centro de Comunicação e Expressão.
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The mechanism of rabbit skeletal muscle phosphoglucomutase (EC.2.7.5.1) has been investigated using fluorinated and deoxygenated substrate analogues. Each of the analogues in which the non-acceptor hydroxyls are replaced by fluorine or hydrogen are substrates of the enzyme. The kinetic constants of these substrates are reported. The rate of the mutase reaction of each substrate analogue in the presence of glucose 1,6-diphosphate is the same as that of the half reaction involving production of the fluorinated and deoxygenated glucose 1,6-diphosphate species. The exceptions are 3-fluoro- and 3-deoxy-glucose 1-phosphate, in which cases the rates of the half reactions are 8 times that of the overall mutase reaction. The Km of 3-fluoro-glucose 1,6-diphosphate is approximately 90 times that of glucose 1,6-diphosphate and the other deoxy and fluoro analogues. The inhibition of phosphoglucomutase by fluorinated and deoxygenated substrate analogues has been investigated. The synthesis of a series of novel disubstituted inhibitors (based on glucose 1-phosphate) in which the C-6 hydroxyl is replaced by fluorine and a sugar ring hydroxyl is replaced by either hydrogen or fluorine is described. The inhibition constants show that the hydroxyl distal to the acceptor hydroxyl is most important in the formation of a strong enzyme-inhibitor complex. The synthesis is described of three phosphorofluoridate analogues of glucose phosphate substrates. These analogues were found to only weakly inhibit phosphoglucomutase. No evidence of any phosphoryl transfer between the phosphoenzyme and the phosphorofluoridate analogues could be detected. Thus phosphoglucomutase has a strict requirement for a doubly negatively charged substrate phosphate group. The interaction of phosphoglucomutase with fluorinated substrates and inhibitors has been investigated by ¹⁹Fnmr. Large downfield changes in the chemical shifts of the inhibitors 6-fluoro-glucose 1-phosphate and α-glucosyl fluoride 6-phosphate were found to accompany binding to the phosphoenzyme. The effects of the binding of activating and non-activating metal ions on these spectra were investigated. The different effects observed may be directly related to the chemical basis for the metal induced activation of the enzyme. ¹⁹Fnmr data consistent with a 10² to 10³ fold increase in the tenacity with which phosphoglucomutase binds substrates and inhibitors in the presence of Li⁺ were observed in the spectra of the phosphoenzyme with difluorinated glucose 1-phosphate inhibitors. Two enzyme bound species were detected in the ¹⁹Fnmr spectra of the complexes formed by reaction of the Cd²+ phosphoenzyme with 2- and 3-fluoro-glucose phosphates. These species are tentatively assigned as the fluoro-glucose 1,6-diphosphate species bound in two different modes to the dephosphoenzyme. Only one bound species was observed in the case of 4-fluoro-glucose phosphates. The environment of each substrate glucose hydroxyl in the active site was probed using ¹⁹Fnmr and the fluorinated glucose phosphate substrates. Data inconsistent with a minimal motion type of mechanism (W.J. Ray, A.S. Mildvan & J.W. Long, Biochemistry 1973,12, 3124) were obtained. The results of the nmr and kinetic studies are consistent with an exchange type of mechanism in which the C-3 hydroxyl plays an important role in the reorientation of the glucose 1,6-diphosphate. The data also suggest that there are two distinct glucose binding sites, one for each substrate and glucose 1,6-diphosphate bound in the same mode.
Science, Faculty of
Chemistry, Department of
Graduate

The meniscus is essential for normal knee function. Previously considered unimportant, removal was performed when injured. This frequently led to degenerative arthritic changes. Today surgeons attempt to repair or minimally resect torn menisci, however many patients are encountered with irreparable tears or previously removed menisci. In these patients meniscale transplantation may prevent degenerative arthritis from developing.
Problems facing transplantation are ability to store tissues and prevent disease transmission. To overcome this, freezing and irradiation effects were examined on 60 rabbits undergoing medial meniscal transplantation. Fresh, frozen, and frozen-irradiated allografts were followed up to 17 months, 10 other rabbits underwent meniscectomy alone.
Zero-time studies revealed that freezing with or without irradiation rendered cells non-viable with no changes in mechanical properties. At long term follow-up, all grafts healed, and showed repopulation with metabolically active cells determined radioautographically. Mechanical properties were unchanged, except for a decreased time constant in the frozen group. Degenerative changes were not significantly different among groups.