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Dissertations / Theses on the topic 'Raffinose oligosaccharides'

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Published: 4 June 2021

Last updated: 1 February 2022

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1

Greenwood, Michelle. "The regulation and metabolism of raffinose family oligosaccharides in malting barley." Thesis, Heriot-Watt University, 2007. http://hdl.handle.net/10399/2101.

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The raffinose family oligosaccharides (RFO) are alpha-galactosyl derivatives of sucrose, and the most common are raffinose, stachyose and verbascose. Raffinose is found exclusively in the embryo. Embryos and endosperms of barley (Hordeum vulgare. Cv. Golden Promise) were studied throughout their development and into maturity to investigate the 'relationships among a-amylase, carbohydrate, abscisic acid (ABA), raffinose synthesis pathway and the onset of desiccation tolerance. The onset of desiccation tolerance corresponds to an increase in starch, a-amylase, raffinose and a decrease in monosac

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2

Cao, Te. "Metabolic Engineering of Raffinose-Family Oligosaccharides in the Phloem Reveals Alterations in Patterns of Carbon Partitioning and Enhances Resistance to Green Peach Aphid." Thesis, University of North Texas, 2010. https://digital.library.unt.edu/ark:/67531/metadc30441/.

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Phloem transport is along hydrostatic pressure gradients generated by differences in solute concentration between source and sink tissues. Numerous species accumulate raffinose-family oligosaccharides (RFOs) in the phloem of mature leaves to accentuate the pressure gradient between source and sinks. In this study, metabolic engineering was used to generate RFOs at the inception of the translocation stream of Arabidopsis thaliana, which transports predominantly sucrose. To do this, three genes, GALACTINOL SYNTHASE, RAFFINOSE SYNTHASE and STACHYOSE SYNTHASE, were expressed from promoters specifi

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3

Unda, Faride. "Evaluating the role of the raffinose family of oligosaccharides in hybrid poplar." Thesis, University of British Columbia, 2012. http://hdl.handle.net/2429/43360.

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The raffinose family of oligosaccharides (RFO) function as transport carbohydrates in the phloem, storage compounds in sink tissues, and as putative metabolic agents that combat plant stresses. Research on the RFO pathway has focused on seed biology and plants that transport raffinose as their primary photoassimilate. In contrast, few studies have explored this pathway in woody species. As such, this thesis investigated the fundamental function of the RFO enzymes in hybrid poplar, with emphasis on galactinol synthase (GolS), an enzyme key to the pathway. Phylogenetic comparisons of Populus Go1

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4

Hagely, Katherine Bray. "Improving soybean seed composition through molecular breeding for Raffinose family oligosaccharides, lectin, and trypsin inhibitors." Thesis, University of Missouri - Columbia, 2013. http://pqdtopen.proquest.com/#viewpdf?dispub=3576094.

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5

Loedolff, Bianke. "Functional roles of raffinose family oligosaccharides: Arabidopsis case studies in seed physiology, biotic stress and novel carbohydrate engineering." Thesis, Stellenbosch : Stellenbosch University, 2015. http://hdl.handle.net/10019.1/98052.

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Thesis (PhD)--Stellenbosch University, 2015.<br>ENGLISH ABSTRACT: The raffinose family of oligosaccharides (RFOs) are α1,6-galactosyl extensions of sucrose (Suc-Galn) unique to the plant kingdom. Their biosynthesis is mediated via α1,6-galactosyltransferases which catalyse the formation of raffinose (Raf, Suc-Gal1), stachyose (Sta, Suc-Gal2) and higher oligomers (Suc-Galn, n ≥ 13) in a stepwise manner. RFOs are well known for their historical roles as phloem translocates and general carbon storage reserves. In recent years their physiological roles have expanded to include potential func

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6

Findling, Sarah Angelika [Verfasser]. "Subcellular Distribution, Regulation of the Synthesis and Functions of Raffinose-Oligosaccharides in Ajuga reptans (Lamiaceae) / Sarah Angelika Findling." Wuppertal : Universitätsbibliothek Wuppertal, 2014. http://d-nb.info/1062996879/34.

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7

Wang, Hongrui. "Developing Novel Methods to Mitigate Freezing Injury in Grapevines." The Ohio State University, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=osu1564743163557437.

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8

Meyer, Thibault. "La dégradation des acides hydroxycinnamiques comme signal de perception de la plante : régulation et rôle dans l’écologie d’Agrobacterium fabrum." Thesis, Lyon, 2018. http://www.theses.fr/2018LYSE1101.

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Les agrobactéries établissent des relations à long terme avec les plantes et ce, dans deux styles de vie différents, rhizosphérique et pathogène (galle du collet). Dans ce mode de vie, les bactéries modifient génétiquement leur hôte et se créent ainsi une niche écologique spécifique (tumeur). La transition entre les deux styles de vie est déclenchée par la perception de signaux végétaux, parmi lesquels des acides hydroxycinnamiques (HCAs) comme l’acide férulique. Or dans l’espèce Agrobacterium fabrum, des gènes spécifiques permettent la dégradation des HCAs. Nous avons émis l’hypothèse que cet

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9

Bruel, Laëtitia. "Identification et caractérisation d'une enzyme bifonctionnelle de Ruminococcus gnavus E1 (AgaSK), présentant une activité [alpha]-galactosidase et une activité kinase." Thesis, Aix-Marseille, 2014. http://www.theses.fr/2014AIXM4309.

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Les α-galactosides sont des glucides non digestibles constitués d'unités galactose liées en α(1,6). Les α-galactosides de la famille du raffinose (RFO) sont, avec le saccharose, les principaux oligosaccharides des légumineuses. Cependant, aucune activité α(1,6)-galactosidase n'est retrouvée au niveau de l'épithélium intestinal humain, les RFO sont donc exclusivement fermentés par les enzymes microbiennes. Ces travaux introduisent une enzyme bifonctionnelle de Ruminococcus gnavus E1, un membre majoritaire du microbiote intestinal humain, présentant une activité α(1,6)-galactosidase/ saccharose

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10

Pimont, Pedro Teixeira. "Caracterização dos genes rafinose sintase e estaquiose sintase em gramíneas." reponame:Repositório Institucional da UFABC, 2018.

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Orientadora: Profª. Drª. Hana Paula Masuda<br>Coorientador: Prof. Dr. Danilo da Cruz Centeno<br>Dissertação (mestrado) - Universidade Federal do ABC, Programa de Pós-Graduação em Biotecnociência, São Bernardo do Campo, 2018.<br>Os oligossacarídeos da série da rafinose (OSRs) são carboidratos formados pela adição sequencial de um grupo galactosil, geralmente doado por uma molécula de galactinol, à molécula de sacarose. Essa via é regulada principalmente por três enzimas. A galactinol sintase (GOLS) que é responsável pela síntese de galactinol. A rafinose sintase (RAFS) que transfere o resíduo g

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11

Zhao, Lu. "Temperature-dependent Regulation of Sugar Metabolism During Cold Stress Responses." The Ohio State University, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=osu1483361544085616.

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12

Cruz, Ana Clara de Oliveira. "Metabolismo de carboidratos em cana-de-açúcar sob déficit hídrico." reponame:Repositório Institucional da UFABC, 2018.

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Orientador: Prof. Dr. Danilo da Cruz Centeno<br>Coorientadora: Profª. Drª. Hana Paula Masuda<br>Dissertação (mestrado) - Universidade Federal do ABC, Programa de Pós-Graduação em Biotecnociência, São Bernardo do Campo, 2018.<br>A busca por fontes renováveis de energia tem aumentado devido à preocupação mundial com o uso excessivo de combustíveis fósseis, destacando-se como alternativa o etanol produzido a partir da cana-de-açúcar. O plantio de cana é intenso no Brasil, por ser uma cultura relativamente bem adaptada às distintas condições locais. No entanto, condições de estresse biótico e abió

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13

Kirsch, Monika. "Low temperature induces raffinose family oligosaccharide and maltose accumulation in plants." Göttingen Cuvillier, 2009. http://d-nb.info/999186418/04.

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14

Glover, Natasha Marie. "The Genetic Basis of Phytate, Oligosaccharide Content, and Emergence in Soybean." Diss., Virginia Tech, 2011. http://hdl.handle.net/10919/77157.

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Soybean [Glycine max (L.) Merr] is one of the U.S.'s most economically important crops due to the protein and oil content of seeds. The major storage form of phosphorus in soybean seeds is found in the form of phytate, but because of its negative nutritional and environmental impacts, seed phytate and raffinosaccharide content have been a recent focus of breeders and molecular geneticists. The soybean line CX1834 is a low phytate mutant known to have two low phytate QTLs on linkage groups (LGs) L and N. The first objective of this research was to determine the genetic basis of the low phytate

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15

Knop, Christian. "Zur Bedeutung von Saccharose-Transportern in Pflanzen mit offener Phloemanatomie." [S.l.] : [s.n.], 2001. http://webdoc.sub.gwdg.de/diss/2001/knop/knop.pdf.

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16

Alvarez, Sophie. "Modifications du protéome et variations de la composition en métabolites : sucres solubles, amidon, acides organiques et proline, au cours de l'acclimatation au froid associées à la tolérance au gel du pois." Lille 1, 2004. https://pepite-depot.univ-lille.fr/LIBRE/Th_Num/2004/50376-2004-87-88.pdf.

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La relation entre l'acquisition de la tolérance au gel et les teneurs en sucres solubles, l'amidon, les acides organiques et la proline a été étudiée chez deux variétés de pois (Pisum sativum L. ): Champagne, tolérant au gel et Térèse, sensible au gel. Le contenu en saccharose et raffinose dans l'appareil aérien et racinaire de Champagne augmente significativement en réponse à l'acclimatation au froid. Cependant chez les 59 lignées recombinantes, issues du croisement Champagne et Térèse cultivées en champ, il semble que l'accumulation des sucres solubles ne garantit pas l'acquisition de la tol

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17

"Raffinose family oligosaccharides (RFO) biosynthesis in chickpea (Cicer arietinum L.) seeds." Thesis, 2014. http://hdl.handle.net/10388/ETD-2014-08-1694.

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To increase the global acceptability of chickpea by improving its nutritional quality, seed RFO (Raffinose Family Oligosaccharides) concentration needs to be reduced without affecting their role during seed development and positive impact on human health. To achieve this objective, the key regulating step(s) of RFO biosynthesis needs to be identified. The three main objectives of the thesis were: (1) to optimize an analytical method to determine soluble sugars concentration in chickpea seeds including RFO, (2) to determine chickpea genotypes with contrasting seed RFO concentration, and (3) to

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