To see the other types of publications on this topic, follow the link: Ram Semen.
Journal articles on the topic 'Ram Semen'
Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles
Consult the top 50 journal articles for your research on the topic 'Ram Semen.'
Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.
You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.
Browse journal articles on a wide variety of disciplines and organise your bibliography correctly.
1
Salamon, S., and W. M. C. Maxwell. "Storage of ram semen." Animal Reproduction Science 62, no. 1-3 (August 2000): 77–111. http://dx.doi.org/10.1016/s0378-4320(00)00155-x.
Full text
2
Oláh, János, Tamás Pécsi, András Kovács, Anna Pécsi, and András Jávor. "Tenability of ram semen." Acta Agraria Debreceniensis, no. 31 (November 24, 2008): 63–66. http://dx.doi.org/10.34101/actaagrar/31/3008.
Full textAbstract:
It could be stated that the diluted semen of Awassi rams taken in the breeding season preserved its fertilizing abilityat different temperatures for different periods of time. The motility of spermatozoa kept at 23 vs. 8oC was checked daily. The largest spread of data was observed 24 hours after taking the semen, then the motility rate of cells showed a linear decrease. Motility results of fresh and frozen-thawed samples were compared also after heat resistance test and significant differences were found between these groups. Significant individual differences were observedin the sperm motility after heat resistance test.
3
Budai, Csilla, István Egerszegi, József Rátky, and András Kovács. "Storage of ram semen in gelatin supplemented extender." Acta Agraria Debreceniensis, no. 48 (July 31, 2012): 7–10. http://dx.doi.org/10.34101/actaagrar/48/2444.
Full textAbstract:
The aim of our study was to examine how different gelatin concentrations affect ram semens viability in liquid storage at 5 oC for five days. Our hypothesis was if we add gelatin to the semen extender, than the viability of ram semen will be better in the extenders containing gelatin, than the control. We used two different semen extenders:1.5% UHT milk and 1.5% UHT milk + 5% egg yolk. We added 0; 0.5; 1.0; 1.5; 2.0% Dr. Oetker gelatin to the semen extenders. We stored the semen for five days at 5 oC and in every 24 hour we made sampling.We stained the smears with Kovács-Foote staining and evaluated them with light-microscope. We categorized the cells in five groups like: live and intact cells, live cells with injured acrosome, dead cells, live head with dead tail and live tail with dead head. We used one-way analysis of variance (ANOVA) to assign how gelatin concentration affects the number of the categorized cells. On the fifth day, the viability was the best in the following semen extenders: 1.5% fat UHT milk + 1.0% gelatin and 1.5% fat UHT milk + 1.5% gelatin, but it was not significant (p>0.05).
4
Ptáček, M., M. Stádníková, F. Savvulidi, and L. Stádník. "Ram Semen Cryopreservation Using Egg Yolk or Egg Yolk-free Extenders: Preliminary Results." Scientia Agriculturae Bohemica 50, no. 2 (June 1, 2019): 96–103. http://dx.doi.org/10.2478/sab-2019-0014.
Full textAbstract:
Abstract Kinematic parameters of thawed ram semen frozen under field conditions were analysed with the use of two commercial (egg yolk vs egg yolk free) semen extenders in different year-seasonal periods. The semen was collected from Suffolk (n = 2) and Charollais (n = 1) rams kept in private breeding farm on 3 test days (pre-mating, at mating, post mating) during year 2016. Two commercial semen extenders (egg yolk-based BullXcell® or egg yolk-free AndroMed®) were used for semen processing. Processed semen was frozen in 0.25 ml plastic cryostraws using the custom-made portable freezing box for ram semen cryopreservation under field conditions. Thawed semen characteristics were evaluated using computer-assisted semen analysis (CASA) system immediately after thawing and after 2 h of heat incubation (±38°C). Significantly higher total motility (+12.3%), straight line velocity (+5.6 μm s−1), and average-path velocity (+6.9 μm s−1) were detected for the semen processed and cryopreserved using egg yolk-based semen extender. Year-seasonal variation and introducing the ram to service had shown to have a significant effect on the cryopreserved ram spermatozoa. These preliminary results confirmed the feasibility of ram semen processing and cryopreservation under field conditions.
5
Swelum, A. A., I. M. Saadeldin, M. Bahadi, M. Afifi, M. Al-Mutary, and A. N. Alowaimer. "The effect of heterologous seminal plasma from ram, buck or camel on the freezability of ram semen." Veterinární Medicína 63, No. 11 (November 19, 2018): 500–512. http://dx.doi.org/10.17221/52/2018-vetmed.
Full textAbstract:
Here, we compared the effects of ram, buck and dromedary camel seminal plasma mixed with TRIS-egg yolk glycerol extender on the freezing preservation of ram semen. Awassi ram semen samples underwent primary evaluation and were then pooled and diluted with the following diluents: TRIS-egg yolk glycerol mixed with (1) whole ram semen as a control (T); (2) ram sperm after seminal plasma removal (W); or (3) ram, (4) buck or (5) camel seminal plasma (R, B and C, respectively). The diluted semen was frozen using liquid nitrogen vapor. Various sperm parameters were evaluated in the frozen semen. Total motility before and after freezing was significantly higher in R, B and C diluents than in T and W diluents. Progressive motility after freezing was significantly higher in R, B, C and T diluents than in W diluent. Vitality after freezing was significantly higher in B than in W diluent. DNA fragmentation before and after freezing was significantly lower in R, B, C and T diluents than in W diluent. Plasma membrane integrity before and after freezing was significantly higher in R, B and C diluents than in W diluent. Sperm abnormalities before freezing were significantly lower in R, B and C diluents than in W diluent. Malondialdehyde concentration was significantly higher in T and W diluents than other diluents. Reduced glutathione concentration was significantly higher in B diluent than other diluents. Moreover, reduced glutathione concentration was significantly higher in C, R and W diluents than in T diluent. Thus, the addition of ram, buck or camel seminal plasma to TRIS-egg yolk glycerol extender improved the quality of frozen ram semen, while seminal plasma removal adversely affected it. Ram, buck and camel seminal plasma had similar effects, with no significant differences between them on the evaluated parameters of frozen ram semen.
6
Zegeye, Zemenu Birhan, Nóra Vass, and Andualem Tomano. "Application of laparoscopic artificial insemination in conventional Lacaune sheep farm using frozen-thawed semen." Acta Agraria Debreceniensis, no. 2 (December 1, 2020): 133–38. http://dx.doi.org/10.34101/actaagrar/2/7113.
Full textAbstract:
The complex anatomical structure of the ewe reproductive tract accompanied with low quality of frozen ram semen for artificial insemination, resulted in a challenge with regard to using superior genotypes for reproductive ovine performance. Hence, improved genetics in ovine management has not been efficiently and widely used especially in undeveloped countries. Therefore, intrauterine semen deposition by laparoscopic insemination should be adopted in the current sheep production systems. Thus, this study aimed to assess the pregnancy rate and lambing rate of ewe inseminated by laparoscopic insemination techniques using frozen-thawed semen. The research used imported frozen semen from two rams of the Lacaune breed. Ewes were grouped according to age in years (1, 2 and 4). Before insemination, the semen was examined microscopically for its motility and viability and thereafter the laparoscopic artificial insemination technique was performed to 19 Lacaune breed ewes using frozen-thawed semen. The overall pregnancy and prolificacy rates were 31.57% and 42.10% respectively. Out of 2 ewes in the 1-year age group that were inseminated, only 1 ewe lambed representing 50%. However, from 16 ewes inseminated of four-year age group, 5 ewes lambed representing 31.25%. Significant difference based on age group was not evaluated due disproportionate of the data, (such that the data included 2 ewes in one-year-old age, 1 ewe in 2-year-old age and 16 ewes in 4-year-old age). Based on the ram semen, 33.33% and 30% of the inseminated ewes were pregnant from ram A and ram B semen respectively. However, in the case of prolificacy rate, 44.44% and 40 % of the ewes lambed from using semen of ram A and B, respectively. There was no significant difference (p>0.05) in pregnancy and prolificacy rates based on semen from the two rams. In conclusion, in this research study, ram semen had no significant effect on pregnancy and prolificacy rates using laparoscopic AI on Lacaune sheep. This could be due to the fact that the rams had very good quality semen. Evaluation of ram semen, accompanied with appropriate ewe selection based on age and rightful deposition of semen could lead to better and more consistent results. Overall this could contribute to the successful application of laparoscopic artificial insemination in Lacaune sheep production systems for enhanced productivity.
7
Amiridis, G. S., A. Lymberopoulos, Sophia Varsakeli, Theodora Kouskoura, and Sophia Belibasaki. "Ram seminal plasma and fertility: Results from an ongoing field study." Acta Veterinaria Hungarica 48, no. 3 (July 1, 2000): 335–41. http://dx.doi.org/10.1556/avet.48.2000.3.10.
Full textAbstract:
The effects of partial replacement of ram semen diluent with ram seminal plasma on the fertility of ewes were studied. Crossbred Chios ewes (n = 152) were assigned to six groups. The oestrous cycles of the ewes were synchronised at the peak (Groups A, B, C and D) and at the end (Groups E and F) of the breeding season by means of intravaginal sponges impregnated with fluorogestone acetate (FGA) for 14 days. Four hundred IU of PMSG were injected intramuscularly at the time of sponge removal. Ewes of Groups A, C and E were artificially inseminated with ram semen diluted with skim milk extender, while those of Groups B, D and F with ram semen diluted with 50% skim milk and 50% ram seminal plasma. The addition of ram seminal plasma induced a significant increase (P < 0.05) in litter size in Groups B and D when compared with that of Groups A and C (1.85 and 1.88 vs. 1.39 and 1.52, respectively). This increase was not significant when insemination was performed at the end of the breeding season (2.0 vs. 1.4). These results indicate that the addition of seminal plasma can influence the fertility of ewes or the fertilising capacity of extended ram semen to some extent.
8
Ahangari, Y. J., M. Nowrozi, and A. Soleimani. "The effect of dilution rates and freezing methods on post-thawing motility of Baluchi ram spermatozoa." Proceedings of the British Society of Animal Science 2002 (2002): 130. http://dx.doi.org/10.1017/s1752756200007869.
Full textAbstract:
For the effective use of Artificial insemination technique in sheep industry, investigation on the methods of ram semen dilution and freezing is necessary. Ahangari (1992) showed that various rates of dilution from 1:1 to 1:4 did not affect p>0.05 post thawing survival of Cambridge ram spermatozoa. Fiser et al (1987) achieved a 73% c.f. 67% pregnancy rate using thawed semen, previously frozen to -100 and -79 respectively. The objective of this study was to investigate the effect of two rates of dilution of semen and two methods of freezing on post-thawing motility of ram spermatozoa.
9
Widiantoro, Krisna, Sri Pantja Madyawati, Trilas Sardjito, Tatik Hernawati, Indah Norma Triana, and Sunaryo Hadi Warsito. "Kualitas post-thawing semen domba Merino dalam bahan pengencer berbasis susu skim-kuning telur yang ditambah isolat crude protein Tirosine Kinase." Ovozoa : Journal of Animal Reproduction 10, no. 2 (August 17, 2021): 39. http://dx.doi.org/10.20473/ovz.v10i2.2021.39-45.
Full textAbstract:
This study was conducted to determine the effect of crude protein tyrosine kinase (PTK) isolates supplementation into skim milk-egg yolk based diluent to maintain the quality of Merino ram spermatozoa. Four ejaculates of two Merino rams were divided into two groups for the control group (P0): Merino ram semen was diluted in skimmed milk-egg yolk based diluent, and the treatment group (P1): Merino ram semen was diluted in skim milk-egg yolk based diluent contained crude PTK 1,597 mg/ml diluent. All diluted semen was equilibrated for 2 hours at 5 °C and filled into 0.25 mL French straws. The filled straws were placed on steel racks (Cooltop, Minitube) held in liquid nitrogen vapour for 10 minutes at –140 °C, immersed immediately in liquid nitrogen at –196 °C, and stored for 48 hours for later assessment. Post-thawed semen samples were evaluated for spermatozoa motility, viability, and morphological abnormality. The results showed that the spermatozoa motility of fresh semen of Merino ram was 82.5 ± 2.89, which was qualified for freezing. The post-thawing spermatozoa motility, viability, and morphological abnormalities of Merino ram in the P1 group were 34.11 ± 3.26%, 38.00 ± 3.00%, and 12.89 ± 4.54%, respectively. It were higher (p <0.05) than the control group of 24.44 ± 2.9%, 26.67 ± 3.32%, and 21.11 ± 3.02%. It was concluded that the addition of crude PTK isolates of 1.597 mg/ml skim milk-egg yolk diluent improved the quality of post-thawed spermatozoa of Merino ram.
10
Windsor, D. P. "Mitochondrial function and ram sperm fertility." Reproduction, Fertility and Development 9, no. 3 (1997): 279. http://dx.doi.org/10.1071/r96109.
Full textAbstract:
These experiments investigated the effect of freezing on mitochondrial function in ram sperm, the effectiveness of current freezing procedures in protecting mitochondria, and the role of mitochondrial respiration in cervical penetration and transit by ram sperm. Only sperm with functioning mitochondria (assessed by rhodamine 123 staining) after freezing and thawing were motile in a viscous medium (P < 0· 05). A simplified rhodamine 123 uptake assay was developed to monitor sperm mitochondrial function. The results of this procedure were highly correlated (r2 = 0 · 98) with the proportion of damaged sperm in the semen sample. A semen freezing procedure commonly used by industry was compared with newer methods, and with freezing without cryoprotectants. None of the freezing protocols produced sperm with higher post-thaw levels of mitochondrial integrity than unprotected sperm. Merino ewes were inseminated with semen treated with metabolic inhibitors. Glycolytic inhibition did not affect fertility. Mitochondrial inhibition reduced fertility in cervically (P < 0 · 05), but not laparoscopically inseminated ewes. It is concluded that mitochondrial respiration plays an important part in penetration of the cervix by ram sperm. Mitochondrial injury during freezing is likely to be implicated in the poor fertility of frozen ram semen used for cervical insemination.
11
Mataveia, G., S. J. Terblanche, J. O. Nöthling, and D. Gerber. "21 EFFECTS OF HETEROLOGOUS SEMEN PLASMA AND SEMEN EXTENDERS ON PROGRESSIVE MOTILITY OF FROZEN - THAWED RAM SPERM." Reproduction, Fertility and Development 17, no. 2 (2005): 160. http://dx.doi.org/10.1071/rdv17n2ab21.
Full textAbstract:
Frozen-thawed ram semen crosses the cervix poorly, necessitating laparoscopic insemination. Acceptable fertility can be achieved with frozen-thawed ram semen deposited at the external cervical opening if ram semen plasma (SP) is added (McPhie et al. 2000 14th ICAR 2, 78 abst). Homologous SP improves the fertility of frozen-thawed sperm of boars and dogs. Heterologous SP may have effects as well; the addition of bovine SP increased the ability of buffalo sperm (Syncerus caffer) to fertilize bovine oocytes in vitro (de Haas et al. 2003 Theriogenology 59, 392). The aim of the current study was to compare the effects of SP of rams (SPR), bulls (SPB), and dogs (SPD), protein-free TALP, Triladyl (Minitüb, Tiefenbach, Germany), and skim milk upon longevity and percentage of progressively motile frozen-thawed ram sperm. Three ejaculates from each of six rams (2 Dorpers, 2 Döhne merinos, and 2 merinos), aged 2–4 years, were extended in Triladyl, pooled and frozen as a single batch per ram at 200 × 106/mL in 0.25-mL straws. SPR was obtained from the same rams and SPB from 5 bulls by centrifugation, while the post-sperm fractions were collected from 5 dogs (SPD). Within a species, the SP from different donors was pooled and frozen in aliquots at −18°C. The 108 straws (6 rams, 6 diluents, 3 replicates) were thawed in random order. Once thawed, a straw was emptied into a tube with 0.85 mL of the appropriate fluid at 37°C and kept for 6 h. Percentage of progressively motile sperm was estimated at ×200 magnification immediately and 2, 4 and 6 h after thawing. One person thawed the semen and prepared motility specimens, while another performed all motility evaluations. Data were evaluated by means of repeated-measures ANOVA, with rams as subjects and time and fluid as fixed effects. Non-significant interactions were removed from the model. Means were compared by means of Bonferroni's test (P < 0.05). The model included ram, time, fluid, and ram × fluid, and time × fluid interactions, which were all significant (P < 0.01). Mean motility decreased from each time to the next and were 39.0% (0 h), 26.0% (2 h), 19.6% (4 h) and 12.6% (6 h), SEM 1.38%, n = 108. Mean motility was higher for skim milk (39.9%) than for all other fluids except Triladyl (27.7%), which was better than SPB (13.0%), whereas TALP (20.5%) and SPR (21.9%) were similar to Triladyl and SPB (n = 72, SEM 2.85%). The interactions (ram × fluid or time × fluid) were mainly due to SPD, SPR, Triladyl, and TALP, while milk resulted in the best and SPB in the lowest motility. This study shows that heat-treated skim milk maintains progressive motility of frozen-thawed ram sperm better than the SP of various species and protein-free TALP. In contrast to SPR, skim milk is known to result in poor fertility of frozen-thawed ram semen after cervical insemination. It would thus appear that maintenance of progressive motility in vitro may be a poor indicator of fertility after cervical insemination.
12
Savvulidi, Filipp, Martin Ptáček, and Luděk Stádník. "Pathogens in Processed Ram Semen and Approaches for Their Elimination." Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis 66, no. 4 (2018): 1065–72. http://dx.doi.org/10.11118/actaun201866041065.
Full textAbstract:
A variety of pathogenic contaminants might be isolated from semen, processed for artificial insemination (AI) programs: prions, viruses, bacteria, yeasts or protozoa. This review will discuss the broad scale of pathogens detected in processed ram semen. Reviewed findings will be confronted with the latest knowledge related to semen contamination in other livestock species. An accent will be placed on current experience and future aspects of approaches for elimination of pathogens from processed semen (especially, from semen processed under field conditions).
13
Godfrey, Robert W., Sabrina Keiper, and Sue Lakos. "78 Evaluation of extended hair sheep ram semen stored as liquid at 5°C." Journal of Animal Science 98, Supplement_2 (November 1, 2020): 74–75. http://dx.doi.org/10.1093/jas/skz397.175.
Full textAbstract:
Abstract This study was designed to evaluate the quality of extended hair sheep ram semen stored as a liquid at 5°C. St Croix White (STX; n = 6) and Dorper x STX (DRPX, n = 5) rams were collected weekly for 3 wk using estrus ewes fitted with an intravaginal collection vial. Semen was kept at 32°C during transport to the lab and during processing. Semen was evaluated for percent motility (MOT), viability (LIVE) using eosin-nigrosin stain and concentration using a hemocytometer counting chamber. Semen was extended to a final concentration of 250 x 106/mL in a one-step dilution with a skim UHT milk extender with 10% egg yolk by volume and packaged into 0.5 mL straws. Straws were stored in a styrofoam box in a refrigerator at 5°C for 96 h, or in an Equitainer® set up using the manufacturer’s instructions, for 24 h at which time they were transferred to the styrofoam box in the refrigerator for 72 h. Semen was evaluated for MOT and LIVE at -1, 0, 24, 48 72 and 96 h relative to cooling. Semen traits were analyzed using GLM of SAS for repeated measures with ram, time, breed and cooling method in the model. There was no difference (P > 0.10) in MOT or LIVE over time between breeds or cooling method. One STX ram did not produce any samples that survived extension; one STX and four DRPX rams produced at least one sample that did not survive extension and all were removed from analysis. The MOT decreased (P < 0.0001) from 81.7 ± 2.9 % at -1 h to 52.2 ± 2.9% at 96 h. The LIVE decreased (P < 0.0001) from 83.1 ± 3.6% at -1 h to 50.4 ± 3.6% at 96 h. These results show that ram semen stored as a liquid at 5°C can maintain motility and viability for 96 h. Further studies will be conducted to evaluate cooling of extended ram semen and fertility after artificial insemination.
14
Anderson, Jennifer M. L., R. F. E. Axford, and I. Ap Dewi. "The effect of selenium supplementation on fresh and frozen ram semen." Proceedings of the British Society of Animal Science 1996 (March 1996): 185. http://dx.doi.org/10.1017/s0308229600031512.
Full textAbstract:
Previous research conducted on bulls, rats and man have shown that selenium-deficient animals produce less viable semen than animals of an adequate status, because the tail of the spermatozoa is a seleno-flagellate (Slaweta et al., 1988). Furthermore, the fertilising ability of ram spermatozoa is reduced in liquid nitrogen as the semen quality is affected by osmolality and the freezing and thawing process (Colas and Guerin, 1981). In a small experiment, the effect of selenium supplementation on low-selenium rams was tested to ascertain the quality and viability of fresh ram semen and the post-thaw recovery and fertilising ability of frozen semen.
15
El-Shahat, Khaled, Magdi Waheed, Amal Hasan Ali, Abdel Aziz Sallam, and Badr El-Saidy. "Influence of sugars and osmoregulators on motility and viability of cooled and frozen-thawed ram semen." Roczniki Naukowe Polskiego Towarzystwa Zootechnicznego 16, no. 2 (June 30, 2016): 51–57. http://dx.doi.org/10.5604/01.3001.0014.2020.
Full textAbstract:
The aim of the study was to determine the effect of sugars and osmoregulators on the viability of frozen-thawed ram semen in a Tris-based diluent. Two experiments were conducted to evaluate the effect of sugars (fructose, glucose, sucrose and raffinose) and two osmoregulators (hypotaurine and taurine) on the viability of ram sperm. In experiment 1, each of the four sugars (fructose, glucose, sucrose and raffinose) was added (500 mg%) to the diluents. Semen was collected from 15 rams once a week using an artificial vagina, diluted, cooled slowly to 5°C over 2 h, frozen in the form of pellets, and plunged into liquid nitrogen. The frozen semen was thawed, and sperm viability was calculated 3 h after thawing. In experiment 2, two osmoregulators, hypotaurine (0.20 and 0.40 mg/ml) and taurine (2 and 4 mg/ml), were added to Tris-based raffinose diluent. Motility and viability rates were calculated. The results showed that motility was gradually significantly (P < 0.05) improved by using 500 mg% raffinose in the Tris-based diluents after dilution at 30°C and before freezing at 5°C. Post-thawing motility and viability rates were highest (P<0.05) when raffinose was used in Tris-based diluent for cryopreservation of ram semen. In vitro supplementation of the semen diluent with 4 mg/ml taurine had a beneficial effect on the motility and viability of ram spermatozoa after dilution at 30°C and before freezing at 5°C. The same trend was observed after freezing-thawing and 3 h post-thawing. In conclusion, 500 mg% raffinose was a more suitable sugar component for treatment of ram spermatozoa in Tris diluent than fructose or glucose, while 4 mg/ml of taurine in Tris-raffinose medium exerted a beneficial effect on the motility and viability of ram sperm at cooling and post-thawing.
16
Janett, F., D. Hüssy, C. Lischer, M. Hässig, and R. Thun. "Semen characteristics after vasectomy in the ram." Theriogenology 56, no. 3 (August 2001): 485–91. http://dx.doi.org/10.1016/s0093-691x(01)00579-9.
Full text
17
Maxwell, WM, and S. Salamon. "Liquid storage of ram semen: a review." Reproduction, Fertility and Development 5, no. 6 (1993): 613. http://dx.doi.org/10.1071/rd9930613.
Full textAbstract:
Research from 1930 to 1992 is reviewed with regard to storage of semen at reduced (0-5 degrees C or 10-15 degrees C) and at ambient temperatures. Diluents investigated have included synthetic buffers combined with sugars and egg yolk or its fractions, milk from various sources, glycine, and other substances. Irrespective of the diluent, dilution rate, temperature or conditions of storage, the spermatozoa deteriorate with time of storage. Changes include reduction in motility and morphological integrity of spermatozoa, accompanied by a decline in their survival in the female reproductive tract, reduction in fertility and increased embryonic loss. In critical studies, fertility declined rapidly when semen stored for more than 24 h was used for cervical insemination, but after intrauterine insemination some spermatozoa maintained their fertilizing capacity up to 10 days. Laparoscopic intrauterine or transcervical inseminations could be the means of improvement of fertility. These methods may eliminate the problem of sperm transport through the cervix and ageing of spermatozoa in the reproductive tract, thereby improving fertilization of ova and reducing embryonic loss.
18
Bittencourt, Rodrigo Freitas, Eunice Oba, Carmo Emanuel de Almeida Biscarde, Hymerson Costa Azevedo, Marta Vasconcelos Bittencourt, Gabriel Felipe Oliveira de Menezes, Adrielle da Silva Lima, Kárita da Mata Fuchs, and Antônio de Lisboa Ribeiro Filho. "Dimethylacetamide and trehalose for ram semen cryopreservation." Cryobiology 85 (December 2018): 1–6. http://dx.doi.org/10.1016/j.cryobiol.2018.10.266.
Full text
19
Puspita, Satya Alysa Cahya, Suherni Susilowati, Trilas Sardjito, Abdul Samik, Indah Norma Triana, and Soeharsono Soeharsono. "Penambahan alfa-tokoferol dalam pengencer susu skim - kuning telur terhadap kualitas spermatozoa domba Sapudi yang disimpan pada suhu 5°C." Ovozoa Journal of Animal Reproduction 9, no. 3 (December 7, 2020): 69. http://dx.doi.org/10.20473/ovz.v9i3.2020.69-76.
Full textAbstract:
Spermatozoa in fresh semen of Sapudi ram has a limited life span. The storage of semen in cold temperatures (5 °C) is intended to prolong the spermatozoa's life. However, storage in cold temperatures can lead to increased production of reactive oxygen species (ROS). This condition reduces the quality of spermatozoa. The purpose of this study was to determine the effect of alphatocopherol supplementation in skim milk-egg yolk extender on viability, motility, and plasma membrane integrity of Sapudi ram spermatozoa. Fresh semen derived from Sapudi ram was divided into four treatment groups. Control treatment (P0): semen was added in the extender of skim milkegg yolk without alpha-tocopherol. Three other treatments: P1, P2, and P3 semen were added in skim milk-egg yolk extender with the supplementation of 0.25, 0.5, and 1 gram alpha-tocopherol/ 100 mL extender, respectively. The results showed that the viability, motility, and integrity of the spermatozoa plasma membrane decreased gradually according to the storage length. Supplementation of skim milk-egg yolk extender with 0.5 gram of alpha-tocopherol/100 mL (P2) was able to maintain spermatozoa quality longer (p <0.05) than the control group. It can be concluded that alpha-tocopherol with a concentration of 0.5 g/100 mL of skim milk-egg yolk extender effectively maintains the quality of Sapudi ram spermatozoa in storage at 5 ° C.
20
Morrier, A., F. Castonguay, and J. L. Bailey. "Glycerol addition and conservation of fresh and cryopreserved ram spermatozoa." Canadian Journal of Animal Science 82, no. 3 (September 1, 2002): 347–56. http://dx.doi.org/10.4141/a01-045.
Full textAbstract:
Fresh extended ram semen has a short fertile lifespan whereas acceptable fertility with cryopreserved semen is achieved only by laparoscopy, which limits widespread artificial insemination in sheep. Although glycerol is considered essential for freezing spermatozoa, it is often included in extenders for short-term storage at above-freezing temperatures. To test the hypothesis that glycerol reduces the function of fresh sperm, ram semen was divided into two aliquots and diluted with commercial extenders that were identical, except that one contained 7% glycerol (n = 6). In a second experiment, ram semen was prepared for cryopreservation by a one-step dilution with a 7% glycerol extender or gradually, with a two-step protocol, to test the hypothesis that the method and time of glycerol addition affects sperm quality after freezing and thawing (n = 7). For both experiments, semen was diluted in a synthetic oviductal fluid (SOF-m) and sperm quality was assessed by computer-assisted motility, viability and chlortetracycline fluorescence (CTC) patterns (an indicator of capacitation status). The presence of glycerol did not affect the quality of fresh sperm (P > 0.27). For cryopreserved sperm, the method of glycerol addition also did not affect thawed sperm. However, a decrease in sperm motility and viability, and different distribution of CTC patterns occurred due to the duration of time in extender and in SOF-m (P ≤ 0.0002). Cryo-capacitation was also observed. In conclusion, the presence of glycerol in the extender did not reduce ram sperm quality during conservation of the semen at 5°C or when it was used to completely and rapidly dilute the semen before cooling for cryopreservation. Key words: Sheep, Triladyl, Biladyl, chlortetracycline, artificial insemination, spermatozoa.
21
NTEMKA, A., I. A. TSAKMAKIDIS, E. KIOSSIS, A. MILOVANOVIĆ, and C. M. BOSCOS. "Current status and advances in ram semen cryopreservation." Journal of the Hellenic Veterinary Medical Society 69, no. 2 (July 18, 2018): 911. http://dx.doi.org/10.12681/jhvms.18014.
Full textAbstract:
Ram semen cryopreservation contributes to genetic improvement through artificial insemination, eliminates geographical barriers in artificial insemination application and supports the preservation of endangered breeds thus the conservation of biodiversity. Sperm freezing process induces ultrastructural, biochemical and functional changes of spermatozoa. Especially, spermatozoa’s membranes and chromatin can be damaged, sperm membranes’ permeability is increased, hyper oxidation and formation of reactive oxygen species takes place, affecting fertilizing ability and subsequent early embryonic development. Aiming to improve ram frozen-thawed semen’s fertilizing capacity, many scientific investigations took place. Among them the composition of semen extenders, was a main point of interest. Semen preservation extenders regulate and support an environment of adequate pH and buffering capacity to protect spermatozoa from osmotic and cryogenic stress. Therefore, permeating (glycerol, dimethyl sulfoxide) and non-permeat ing (egg yolk, skimmed milk) cryoprotectants, sugars (glucose, lactose, trehalose, raffinose), salts (sodium citrate, citric acid) and antioxidants (amino acids, vitamins, enzymes) have been added and tested. Moreover, semen dilution rate, storage temperature, cooling rate and thawing protocol, are also some key factors that have been studied. The research results of this scientific topic are encouraging, not only about the freezing and thawing procedures, but also about the improvement of the additives’ properties. However, further research is needed to enhance the fertilizing ability of ram frozen-thawed semen, making its use practical in sheep reproductive management by the application of cervical artificial insemination.
22
Attila Németh, Sándor Mihályfi, and Tamás Szabados. "Analysis of small ruminants’ semen under the cooling, deep-freezing and sex-orientation method." Acta Agraria Debreceniensis, no. 40 (December 1, 2010): 53–58. http://dx.doi.org/10.34101/actaagrar/40/2706.
Full textAbstract:
The aim of this study was to examine the influence of cooling, deep-freezing and sex-orientation methods on fertility of ram and buck semen. It was pointed out that deep-freezing and sexorientation methods had a more considerable destruction on both semen compared with the effect of cooling method. However, with the development of the sex-orientation method, the results of lambing had a significant increase in sheep. On the other hand, the NRR of the inseminations with deep-frozen ram semen exceeded most of previously publicated results. Being interesting, and hopefully useful benefit in practice that the analysed buck semen samples are shown more favourable results in all methods compared with the same results of rams.
23
Suttiyotin, P., and CJ Thwaites. "Comparison of a swim-up technique with the Hamilton Thorn Motility Analyser for measurement of sperm velocity and motility." Reproduction, Fertility and Development 4, no. 2 (1992): 153. http://dx.doi.org/10.1071/rd9920153.
Full textAbstract:
A colorimeter-based swim-up (SU) technique was developed and compared with a Hamilton Thorn Motility Analyser (HTM) for the evaluation of ram semen. SU parameters (sperm velocity in microns s-1, proportion of rapidly moving spermatozoa, and motility index) were significantly correlated (r from 0.47 to 0.98, P from < 0.05 to < 0.001) with most HTM parameters in semen containing known proportions of motile and immotile spermatozoa and in semen that was cold shocked, aged, diluted-stored and frozen-thawed. There were, however, no significant correlations between sperm velocity and HTM parameters in the fresh semen studied, possibly because of the small number of observations (n = 21) and narrow range of semen quality (83 +/- 2% subjectively scored motility) available. It is concluded that the swim-up technique is a simple, cheap, objective and reliable means of measuring sperm velocity and motility in ram semen.
24
Ahmed, E., MS Islam, MGS Alam, PK Jha, S. Ghosh, N. Naher, and FY Bari. "Bacterial contamination of ram semen used for artificial insemination in indigenous ewes." Bangladesh Veterinarian 34, no. 1 (October 28, 2018): 20–26. http://dx.doi.org/10.3329/bvet.v34i1.38709.
Full textAbstract:
Ram semen was assessed for quality and presence of bacteria. Four ejaculates were collected from each of four rams twice a week using artificial vagina. The volume varied from 0.4 - 1.3 mL, colour from 2 - 4 (creamy to creamy-grey), mass activity from 3 - 5, sperm motility from 75 - 85%, viability from 80 - 95%, and concentration from, 2500 - 5000 × 106/mL. The mass activity of ram R6 was significantly (P<0.05) higher (5.0 ± 0.0) compared with ram R1 (4.4 ± 0.5), R2 (3.9 ± 0.0) and R5 (4.7 ± 0.5). The mean motility was 81.7 ± 4.0, viability 90.0 ± 4.0 and concentration 3519.0 ± 545.6 x 106/ml. E. coli and Staphylococcus spp. were found in all four rams’ fresh semen confirmed by culture, staining and biochemical tests. However, Bacillus spp. was found only in ram R5. When the semen samples were treated with antibiotics there was no growth of bacteria after three days of incubation. It is suggested antibiotics control the transmission of microorganisms through AI in ewes.Bangl. vet. 2017. Vol. 34, No. 1, 20-26
25
Immelda, Kicky Hanis, Suherni Susilowati, and Ira Sari Yudaniayanti. "PENGARUH BAHAN PENGENCER SARI KACANG KEDELAI (Glycine max) TERHADAP VIABILITAS DAN NEKROSIS SPERMATOZOA DOMBA SAPUDI." Ovozoa : Journal of Animal Reproduction 8, no. 1 (April 6, 2020): 36. http://dx.doi.org/10.20473/ovz.v8i1.2019.36-42.
Full textAbstract:
The purpose of study was to find out the effect of different concentrations of soya bean extractin sperm diluter, stored at 5°C toward sperm viability and necrosis. The semen was divided into four groups : P0: 0.1 ml sapudi ram + 1 ml of diluent yolk citrate, P1: sapudi ram 0.1 ml + 1 ml diluent soybeans extract konsentration 5%, P2: 0.1 ml sapudi ram + 1 ml diluent soybeans extract konsentration 10%, P3: 0.1 ml sheep semen + 1 ml diluent soybeans konsentration 15%. The result showed that the dose of soya bean extract diluter konsentration of 15% could increase the viability percentage and could decrease the necrosis percentage of sperm.
26
Lloyd, R. E., A. Fazeli, P. F. Watson, and W. V. Holt. "The oviducal protein, heat-shock 70-kDa protein 8, improves the long-term survival of ram spermatozoa during storage at 17°C in a commercial extender." Reproduction, Fertility and Development 24, no. 4 (2012): 543. http://dx.doi.org/10.1071/rd11173.
Full textAbstract:
Poor fertility rates are often observed when fresh ram semen stored in conventional extenders is used for cervical artificial insemination (AI). Heat-shock 70-kDa protein 8 (HSPA8), found within the oviduct, prolongs boar, ram and bull sperm survival at body temperatures in vitro. Here, we aimed to determine whether supplementing extenders (INRA-96 and RSD-1) with HSPA8 (4 µg mL–1) would improve their performance in maintaining freshly collected ram sperm viability and sperm nuclear DNA integrity during storage over 48 h at 17°C. Sperm function was assessed at 1, 6, 24 and 48 h and this experiment was repeated using 25 × 106 and 800 × 106 spermatozoa mL–1. INRA96 supplemented with HSPA8 maintained sperm viability significantly better than INRA96 alone at both sperm concentrations. However, sperm nuclear DNA fragmentation (DF) increased significantly during storage using the higher sperm concentration, irrespective of the extender and the protein treatment used. Increasing levels of sperm nuclear DF over time could explain why poor fertility rates are often observed following cervical AI using stored ram semen. However, further research is required to ascertain whether supplementing the commercially available INRA96 extender with HSPA8 will improve fertility rates following cervical AI using stored ram semen.
27
Veress, L., Zs Tasi, T. Pécsi, S. Babik, Irén Horváth, and K. Magyar. "Zootechnical and Genetic Aspects of a Prolific Merino Program." Acta Veterinaria Hungarica 47, no. 1 (January 1, 1999): 17–31. http://dx.doi.org/10.1556/avet.47.1999.1.2.
Full textAbstract:
In a Prolific Merino nucleus herd of 200 ewes the ovulation rate (OR) test results obtained in 169 animals between 1988 and 1993 were compared with those of 113 ewes from the same herd in 1996. Whereas earlier the ratio of individuals showing an OR ≥ 4 was only 32%, that of the group checked in 1996 was 59%. This increase could be attributed to 40 ewes, both of whose parents had proven to be homozygous carriers of the prolific gene. To develop the Prolific Merino breed, 21 Booroola Merino rams were imported from New Zealand, and mostly their frozen semen was used. Of these rams, one was not a prolific gene carrier, 8 were homozygous carriers, 10 were heterozygous carriers and two had not been identified yet. Of the 36 home-bred rams, 9 proved to be homozygous by parents, 11 heterozygous, 8 homozygous, one proved to be a non-carrier, and 7 rams and their frozen semen were to be progeny tested. Six thousand doses of frozen semen from a total of 33 animals (16 imported rams and their 17 home-bred offspring) are stored in plastic straws. Sixty-three % of this is semen reserve from rams of the FecBFecBgenotype, belonging to 10 ram lines. The remaining 37% is gene reserve intended for creating homozygous ram lines. Only one ram (no. 3244) was bought for the nucleus herd, the other ram lines were introduced into the herd by assortative mating, using intrauterine insemination. The average conception rate found after 472 intrauterine inseminations was 53% with large (occasionally 10-100%) individual ram differences.
28
Hossain, A., MM Islam, F. Naznin, RN Ferdousi, FY Bari, and NS Juyena. "Quality of ram spermatozoa separated with modified swim up method." Bangladesh Veterinarian 33, no. 2 (April 24, 2018): 62–70. http://dx.doi.org/10.3329/bvet.v33i2.36459.
Full textAbstract:
Semen was collected from four rams, using artificial vagina and viability%, motility% and plasma membrane integrity% were measured. Fresh ejaculates (n = 32) were separated by modified swim-up separation using modified human tubal fluid medium. Four fractions of supernatant were collected at 15-minute intervals. The mean volume, mass activity, concentration, motility%, viability%, normal morphology and membrane integrity% (HOST +ve) of fresh semen were 1.0 ± 0.14, 4.1 ± 0.1 × 109 spermatozoa/ml, 85.0 ± 1.3, 89.4 ± 1.0, 85.5 ± 0.7, 84.7 ± 0.5 respectively. There was no significant (P>0.05) difference in fresh semen quality parameters between rams. The motility%, viability% and HOST +ve % of first, second, third and fourth fractions were 53.4 ± 0.5, 68.2 ± 0.3, 74.8 ± 0.3 and 65.5 ± 0.4; 55.5 ± 0.4, 66.2 ± 0.4, 74.5 ± 0.3 and 73.6 ± 0.3 and 66.7 ± 0.5, 66.8 ± 0.5, 65.2 ± 0.4 and 74.7 ± 0.5 respectively. The motility%, viability% and membrane integrity% of separated semen samples differed significantly (P<0.05) between four fractions. The mean motility% and viability% were significantly higher (P<0.05) in third fraction (74.8 ± 0.3%), whereas the mean HOST +ve% was significantly higher (P<0.05) in fourth fraction (74.7 ± 0.5). All quality parameters of separated spermatozoa were significantly (P<0.05) lower than that of fresh semen. The pregnancy rates were higher with fresh semen (71%) in comparison to that of separated sample (57%).Bangl. vet. 2016. Vol. 33, No. 2, 62-70
29
Al-Anazi, Y., M. G. Al-Mutary, M. M. Alfuraiji, M. Al-Ghadi, A. R. Al-himaidi, and A. Ammari. "Effect of Ram Breed on the Efficiency of in vitroDevelopment of Sheep Embryos." Biosciences, Biotechnology Research Asia 14, no. 4 (December 25, 2017): 1309–13. http://dx.doi.org/10.13005/bbra/2574.
Full textAbstract:
The aim of this work was to investigate the impacts of ram breed on in vitro embryo development from fresh or frozen semen. Semen was collected from Najdi and Naimi rams and frozen; the mass and progressive motility of the spermwere assessed in each trial before and after freezing. Then, 970 oocytes in six replicates were fertilized with fresh and frozen semen in vitro. Different stages of sheep embryos were recorded. There were no significant differences in mass and progressive sperm motility of fresh or frozen ram semen between Najdi and Naimi,but there were significant differences between frozen and fresh semen within each breed. Our results showed significant (P<0.05) differences in 2-cell stage, 4-cell stage, 8-cell stage, morula, fragmented embryos, cleavage and blastocyst rates in the frozen semen group compared to fresh semen group in both breeds. In addition, significant (P<0.05) differencesbetween the two breeds were shown in 8-cell and16-cell embryonic stages.In conclusion, there were slight breed effects on the efficiency of in vitro development of sheep embryos.
30
Arruda, Lúcia Cristina Pereira, Girliane Regina da Silva, André Mariano Batista, Ellen Cordeiro Bento da Silva, Helder Melo de Souza, Giovanna Isabella de Souza Couto, Tânia Maria Sarmento da Silva, and Maria Madalena Pessoa Guerra. "Effect of trolox added to freezing extenders over goat and ram spermatozoa." Research, Society and Development 10, no. 5 (April 29, 2021): e10310514764. http://dx.doi.org/10.33448/rsd-v10i5.14764.
Full textAbstract:
Semen cryopreservation is responsible for decrease the gamete fertility, due to structural and functional damages. Among the various causes, oxidative stress, resulting from the higher generation of reactive oxygen species (ROS), has been attributed to affect semen quality. Thus, it was objectified evaluate the effect of Trolox on ram and goat sperm, subjected to freezing. Semen pools of goat (n=5) and ram (n=6) were diluted in skimmed milk (7% glycerol) or Tris-egg yolk (5% glycerol) extender, respectively, added or not of Trolox (0, 20 or 40 μM/ml) and frozen. After thawing (37 °C/30 s), aliquots of semen were evaluated for lipid peroxidation by high performance liquid chromatography, coupled with a photodiode array detector (HPLC-DAD), and flow cytometry (C11-BODIPY581/591), besides of plasma membrane and acrosome integrity by fluorescence microscopy, and sperm kinetics by computerized sperm analysis (CASA). The antioxidant treatment with Trolox did not determine significant effects (p>0.05) on lipid peroxidation, plasma membrane integrity, acrosomal integrity and on the kinetic parameters evaluated. Thus, it is concluded that Trolox (20 or 40 μM) did not have a protective or deleterious effect on goats and ram sperm, submitted to freezing.
31
Al-Nakib, F. M. S., G. A. Lodge, and J. B. Owen. "A study of sexual development in ram lambs." Animal Science 43, no. 3 (December 1986): 459–68. http://dx.doi.org/10.1017/s0003356100002671.
Full textAbstract:
ABSTRACTTwo experiments were designed to study the effect of rearing method, female company, and repeated libido and semen tests on the pattern of sexual development of ram lambs. Four artificial rearing treatments (AR) were compared with natural rearing (NR) in one experiment and four artificial compared with three natural rearing treatments in a second experiment. Lambs on AR treatments in both experiments, as well as naturally reared lambs in the second experiment, were reared with or without females either before and/or after weaning. Half the lambs in the second experiment were libido and semen tested, and their mean plasma testosterone level was also measured, while the rest were only tested at the end of the experiment. The effect of method of rearing and female company on body growth, testes diameter, libido and semen quality were also investigated.A close relationship was found between body weight and sexual characteristics (for example, testis diameter, semen traits, libido, testosterone level and age at the onset of sexual maturity). NR resulted in faster growth than AR but was not appreciably different from early weaned-naturally reared treatments. It was also observed that mating performance improved steadily with advancement in age and increase in body weight. The onset of sexual maturity was attained at a minimum of 23 kg body weight and a minimum of 198 days of age. However, the heavier the lamb when sexually mature, the better its mating performance.Previous libido tests of ram lambs improved their subsequent mating performance, but had no effect on semen quality. However, libido tests did not improve the performance of those ram lambs which did not exhibit initial libido.It is suggested that early weaning at 2 months of age could be a suitable system for ram lambs weaned off their mothers for breeding purposes. These lambs should be reared as an all-male group but libido tested at monthly intervals commencing at 3 months of age. The criteria for selecting these lambs at an earlier age should be body weight and testis diameter.
32
Robertson, L., and P. F. Watson. "Calcium transport in diluted or cooled ram semen." Reproduction 77, no. 1 (May 1, 1986): 177–85. http://dx.doi.org/10.1530/jrf.0.0770177.
Full text
33
Murawski, Maciej, Tomasz Schwarz, Joanna Grygier, Krzysztof Patkowski, Zdzisław Oszczęda, Igor Jelkin, Anna Kosiek, et al. "The utility of nanowater for ram semen cryopreservation." Experimental Biology and Medicine 240, no. 5 (December 8, 2014): 611–17. http://dx.doi.org/10.1177/1535370214557219.
Full text
34
Liu, Gang, Bin Pan, Shubin Li, Jingyu Ren, Biao Wang, Chunyu Wang, Xiulan Su, and Yanfeng Dai. "Effect of bioactive peptide on ram semen cryopreservation." Cryobiology 97 (December 2020): 153–58. http://dx.doi.org/10.1016/j.cryobiol.2020.08.007.
Full text
35
Maxwell, W. M. C., and P. F. Watson. "Recent progress in the preservation of ram semen." Animal Reproduction Science 42, no. 1-4 (April 1996): 55–65. http://dx.doi.org/10.1016/0378-4320(96)01544-8.
Full text
36
Ahangari, Y. J., R. E. F. Axford, and I. ApDewi. "The use of a hyper-osmotic diluent for freezing ram semen." Proceedings of the British Society of Animal Production (1972) 1993 (March 1993): 60. http://dx.doi.org/10.1017/s0308229600023874.
Full textAbstract:
When semen is frozen ice forms extracellularly, and water is drawn from the cells (Mazur, 1970). Rapid freezing reduces dehydration and causes intracellular ice to form more quickly (Mazur, 1980). Cells frozen rapidly in liquid nitrogen do not survive, suggesting that partial dehydration is necessary for spermatozoa to survive freezing (Fahy et aL, 1984). Watson (1979) suggested that non-penetrating sugars have protective effects on spermatozoa during freezing. The addition of a non-penetrating solute such as raffinose to semen will dehydrate the cells by its osmotic effect.The object of this study was to investigate the effect of raffinose on cryoinjury of ram spermatozoa.Experiment 1.Raffinose was added to Tris egg-yolk glycerol buffer (Evans and Maxwell (1987) to concentrations of 0mM, 33mM, 66mM, 99mM, 132mM, 165mM and 198mM raffinose and used to extend (2:1) pooled semen from Cambridge rams.Factorial experiments with 3 replications were set up to test the effect of raffinose concentration, glycerol concentration, method of packaging of semen, pellet size and cooling rate of straws on the motility and survival of ram semen before and after freezing/thawing.
37
Hegedűšová, Zdeňka, Ladislav Štolc, František Louda, Libor Čunát, and Jan Vejnar. "Effect of different extenders on ram sperm traits during storage." Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis 60, no. 6 (2012): 111–16. http://dx.doi.org/10.11118/actaun201260060111.
Full textAbstract:
The aim of the study was to test commercial extenders used for short-term and long-term sperm preservation. Semen was collected in the reproduction season, i.e. from June to December. The ejaculates were obtained from single services and the routine analysis of the semen was performed immediately after the collection. The examination included semen volume, colour and texture, sperm concentration and motility, ejaculate turbulence and percentage of sperm with abnormal morphology. The semen was diluted with an extender in the ratio of 1:4. The processed semen was transported in an insulated container at 16–18 °C to the laboratory and stored in a stationary thermostat under the same temperature. Sperm motility tests were performed 24, 48, 72 and 96 hours after the placement in to thermostat. Ejaculates diluted with Ovipro, Optidyl, Triladyl and Andromed CSS gave very good results of viability (81.23 %–83.41 %) after 24 hours of storage. After 48 hours, Ovipro, Andromed, Optidyl and Triladyl gave values above 75 %. The Triladyl extender proved to be a good stabilizing agent, showing consistent results during a long-term storage. It was chosen as a control one for overall assessment. Other preservation media did not show any improving or worsening effects. The extender Ovipro showed a high motility effect in the first 48 hours only, and hence it appears to be the best solution for the short-term preservation.
38
Ahangari, Y. J., R. F. E. Axford, and I. Ap Dewi. "A spectrophotometric method for the assessment of vigour of ram semen." Proceedings of the British Society of Animal Science 1995 (March 1995): 136. http://dx.doi.org/10.1017/s0308229600029020.
Full textAbstract:
An ideal method of assessment of semen would be simple, rapid and objective with a high correlation with the ability of semen sample to cause conception in the ewes (Ahangari, 1992). Currently the proportion of motile spermatozoa is determined by visual microscopic appraisal. Although, this method of assessment is rapid and easy, it is subjective. Jasko et al (1989) described a spetrophotometric procedure for measuring equine spermatozoan motility objectively. In the present study a modification of the method of Jasko et al (1989) was tested and applied to ram semen.
39
Hunton, J. R., Sally E. Flecker, and W. M. C. Maxwell. "Pregnancy rates following intra-uterine insemination with pellet or straw-frozen ram semen." Journal of Agricultural Science 109, no. 1 (August 1987): 189–91. http://dx.doi.org/10.1017/s0021859600081144.
Full textAbstract:
There has been considerable research on techniques for artificial insemination of sheep with frozen ram semen (Maxwell, 1984). Acceptable pregnancy rates were reported following ‘two-step’ dilution and freezing of semen in P.V.C. ‘ministraws’ (Colas, 1975; Colas & Guerin, 1981); however, other workers have obtained poor fertility following cervical insemination with semen frozen in straws (Maxwell, etal. 1980; Tervit elal. 1984).
40
Suharman, Herdis. "KUALITAS SEMEN BEKU DOMBA GARUT (Ovis aries) PENAMBAHAN SUKROSA DALAM PENGENCER SEMEN KUNING." BERITA BIOLOGI 16, no. 1 (July 7, 2017): 31–38. http://dx.doi.org/10.14203/beritabiologi.v16i1.2294.
Full textAbstract:
The objective of this research was to examine the effect of sucrose in improving the quality of the plasma membrane intact and sperm motility of frozen semen of Garut ram. Semen was collected using artificial vagina weekly from six mature garut rams. Immediately after initial evaluation, fresh semen was divided into four parts and diluted with Tr s extender without sucrose (T0), Tris extender + sucrose 0.2g/100 ml (T1), Tris extender + sucrose 0.4g/100 ml (T2) and Tris extender + sucrose 0.6g/100 ml (T3), respectively. Results of this research showed that the percentage of sperm motility after thawing in T2 (49.00 ± 5.48%) was significantly (P<0.05) higher than T0 (42.00 ± 2.74%) but was not significantly difference (P>0.05) than T1 (46.00 ± 4.18%)and T3 (48.00 ± 4.47%). Evaluation of plasma membrane intact showed that T1 (62.33 ± 6.51%) was s gnificantly different (P <0.05) w h T0 (49.40 ± 2.19%) but was not significantly different (P> 0.05) than T2 (58.50 ± 4.97%) and T3 (56.40 ± 5.90%). In conclusion, he addition of sucrose in semen extender improved the quality of frozen semen of Garut ram. Concentration of 0.2g / 100 ml is the op ma dose to improve the quality of the plasma membrane intact and motility of spermatozoa during the freezing process.
41
Galarza, D. A., M. Ladrón de Guevara, P. Beltrán-Breña, M. J. Sánchez-Calabuig, A. López-Sebastián, J. Santiago-Moreno, and D. Rizos. "137 Assessment of fertilizing ability of Merino ram semen cold stored up to 48h by heterologous IVF of bovine oocytes." Reproduction, Fertility and Development 31, no. 1 (2019): 194. http://dx.doi.org/10.1071/rdv31n1ab137.
Full textAbstract:
The use of cold-stored ram semen has been applied in sheep AI programs, because it preserves its fertilizing ability similar to fresh. Besides, the heterologous IVF has been successfully employed to assess semen fertilizing ability in several species. Hence, we aimed to evaluate the fertilizing ability of ram semen cold stored up to 48h at 5°C by assessing heterologous IVF using bovine oocytes. Fifteen pools of 3 normospermic Merino ram (2-7 years) ejaculates were collected using artificial vagina, diluted to 200×106 spermatozoa mL−1 with ultra-heat-treatment-based extender (skim milk-6% egg yolk) and cold stored up to 48h. In vitro matured zona-intact bovine oocytes were subjected to heterologous IVF using fresh semen (FS, n=707), semen cold stored to 24h (CS24, n=832) or semen cold stored to 48h (CS48, n=611). In parallel, homologous IVF (control, n=1356) and parthenogenesis (parth control non-fertilized oocytes, n=334) were performed. Ram non-selected and selected (BoviPure, Nidacon International, Mölndal, Sweden) semen parameters were evaluated by computer-assisted semen analysis. Sperm-oocyte interaction was assessed at 2.5h post-insemination (hpi) by evaluating the number of bound spermatozoa, whereas penetration and polyspermy were evaluated after 12 hpi. Presumptive zygotes were fixed and stained with Hoechst 33342 at 18, 20, 22, 24 and 26 hpi to assess pronuclear formation using phase contrast and confocal microscopy. Cleavage rate was evaluated in all groups at 48 hpi. Data obtained from 5 replicates were analysed using one-way ANOVA. Data was expressed as mean±standard error of the mean. In terms of sperm storage time, non-selected semen showed a significant decrease (P<0.05) for CS24 and CS48 compared with FS on progressive motility [SPM (%): 52.30±4.1 and 36.9±5.5v. 71.3±1.6] and straight-line velocity (mm s−1: 132.2±6.1 and 109.7±6.3v. 176.7±4.3), respectively. However, selected semen showed a decrease (P<0.05) only for CS48 when compared with CS24 or FS on SPM (35.6±3.9v. 56.1±6.91 and 59.3±2.6) and straight-line velocity (83.5±4.4v. 105.3±6.5 and 110±2.0), respectively. No differences were observed between heterologous IVF groups in all parameters evaluated. Homologous IVF showed a higher percentage of penetration only when compared with heterologous FS group (44.4±6.8v.12.5±4.5%; P<0.01). The polyspermy was higher in heterologous CS24 group when compared with homologous IVF (11.4±3.4v. 3.8±2.2; P<0.05). The homologous IVF group, as expected, showed the higher percentage of pronuclear formation at 18 hpi compared with heterologous IVF with FS (67.3±5.8v. 35.2±5.6%), CS24 (72.1±4.5 v. 37.2±5.7%) and CS48 (63.0±6.0 v. 27.0±5.6%), respectively (P<0.001). Likewise, cleavage rate was higher in homologous group compared with heterologous IVF and parthenogenetic groups for FS (78.3±2.6.8v. 46.3±3.2 and 7.0±2.3%), CS24 (78.4±2.6 v. 48.3±3.2 and 4.9±2.0%), and CS48 (78.4±3.3 v. 43.3±3.5 and 4.3±1.2%), respectively (P<0.001). In conclusion, Merino ram semen cold stored up to 48h maintains its fertilization ability to the same extend as fresh and can be used for sheep crossbreeding programs.
42
Toishibekov, M. M., M. T. Jazkbayev, and B. B. Molzhigitov. "55 EFFECT OF TREHALOSE FOR FROZEN–THAWED RAM SPERM MOTILITY PARAMETERS." Reproduction, Fertility and Development 27, no. 1 (2015): 120. http://dx.doi.org/10.1071/rdv27n1ab55.
Full textAbstract:
Computer-assisted sperm analysers have become the standard tool for evaluating sperm motility because they provide objective results for thousands of mammalian spermatozoa. Ram semen was collected using electro-ejaculation from 10 adult rams of Chingizskaya indigenous sheep breed. Motility was determined using computer-automated semen analysis (Hamilton Thorne Motility Analyzer, Beverly, MA, USA). Trehalose solution (0.375 M) was added to Tris-buffered saline solution to give the following trehalose extenders: 25, 50, 75, and 100% (vol:vol), and analysed for motility using computer-automated semen analysis. The sperm pellets were resuspended at 24°C in cooling extender – trehalose extenders of each concentration containing 5% egg yolk. The diluted semen was cooled to 5°C within 2 h. The semen was then further diluted 1 : 1 with freezing extender – each trehalose extender containing 1.5% glycerol to obtain a sperm concentration of 2.0 × 108 cells mL–1 – and then loaded into 0.5-mL straws. Straws were frozen using a programmable freezer with a freezing curve of 5°C to –5°C at 4°C per min, –5°C to –110°C at 25°C per min, and –110°C to –140°C at 35°C per min, and then the straws were plunged into liquid nitrogen for storage. Frozen samples were thawed in a 37°C water bath for 30 s and analysed for motility using computer-automated semen analysis. Statistical analyses were performed with a Student's test. The fresh semen samples showed the next results: motility 88.3 ± 2.4%, progressive motility 26.8 ± 6.9%, and progressive velocity 61.9 ± 4.2 μm s–1. Motility of the frozen-thawed spermatozoa was 63.6 ± 2.9% (25% trehalose), 55.6 ± 5.2% (50%), 32.4 ± 4.7% (75%), and 23.6 ± 3.2 (100%). Progressive motility was 15.6 ± 3.9% (25%), 13.7 ± 3.7% (50%), 4.5 ± 1.3% (75%), and 5.2 ± 1.3% (100%). Progressive velocity was 93.5 ± 8.3 μm s–1 (25%), 85.4 ± 8.1 μm s–1 (50%), 65.7 ± 6.1 μm s–1 (75%), 35.2 ± 3.3 μm s–1 (100%). Motility of the frozen-thawed spermatozoa significantly decreased with increasing concentrations of trehalose in the extender (P < 0.05). These preliminary studies showed that further research is needed of use trehalose for ram spermatozoa cryoconservation.
43
Morrier, A., F. Castonguay, and J. L. Bailey. "Conservation of fresh ram spermatozoa at 5°C in the presence of seminal plasma." Canadian Journal of Animal Science 83, no. 2 (June 1, 2003): 221–27. http://dx.doi.org/10.4141/a02-060.
Full textAbstract:
Seminal plasma aids sperm transport and contains factors beneficial for sperm function. In artificial insemination, however, diluting the semen reduces the concentration of seminal plasma. To test the hypothesis that supplemental seminal plasma in extended ram semen improves conservation at 5°C, we added various concentrations of seminal plasma to semen during storage, and investigated subsequent sperm function in vitro. Semen was divided into three aliquots, extended in a commercial diluent (Triladyl) supplemented with 0, 10 or 25% (vol:vol) ovine seminal plasma and cooled to 5°C. After 8 and 24 h at 5°C, sperm were suspended in a modified synthetic oviduct fluid (SOF-m) at 39°C to mimic the female genital tract at insemination. Sperm aliquots were assessed for motility and chlortetracycline fluorescence after 0, 4 and 8 h in the SOFm. No significant differences were observed due to seminal plasma supplementation during conservation at 5°C or incubation in SOF-m at 39°C. However, decreased sperm motility and fewer non-capacitated sperm were observed concomitant with an augmentation of capacitated and acrosome-reacted cells during incubation in SOF-m. Therefore, the hypothesis that diluent supplementation with homologous seminal plasma improves ram sperm conservation or subsequent sperm function was not supported. Key words: Ovine, ram, sperm, motility, viability, chlortetracycline fluorescence, artificial insemination, SOF
44
King, B. J., V. V. Da Silva, J. D. Harper, C. J. Scott, and M. N. Sillence. "220. Equine growth hormone enhances motility and extends longevity of ram spermatozoa in vitro." Reproduction, Fertility and Development 17, no. 9 (2005): 85. http://dx.doi.org/10.1071/srb05abs220.
Full textAbstract:
Sperm survival in vitro decreases with time at room temperature, but may be improved by treatment with recombinant bovine growth hormone in rats1, bulls2 and horses3. Two experiments investigated the effect of equine growth hormone (eGH) on the longevity of ram spermatozoa in vitro. The aim of experiment 1 was to determine if the addition eGH would improve the motility of ram spermatozoa after 24 h and identify any interaction with semen dilution rates used for ram semen preservation. Semen was collected from five mature Merino rams. Ejaculates were assessed for good quality and were diluted 1+50, 1+4, 1+3, 1+2 (semen+diluent) with a Tris-based cryoprotectant. Aliquots from each ram were mixed with eGH to achieve a final concentration of 100 ng/mL eGH and stored at 20°C for 24 h. Motility of spermatozoa was then assessed manually. eGH improved the motility of spermatozoa at all dilution rates compared to controls (P < 0.0001) but most markedly in the 1 + 3 and 1 + 2 samples (42.6 ± 0.8% eGH v. 22.7 ± 2.6% control; 40.5 ± 1.4% eGH v. 22.7 ± 2.2% control, respectively, P < 0.01). The aim of experiment 2 was to determine the optimum eGH concentration for improving sperm motility. eGH was added to aliquots of diluted semen (1+3 dilution rate) to produce samples with final concentrations of 1000, 100, 10 and 1 ng eGH/mL. The samples were placed in a water bath at 20°C for 24 h at which time the motility of sperm was assessed as before. Sperm motility was higher in the 100 ng/mL eGH sample (P < 0.05; 39.6 ± 0.7%) compared to other concentrations (1000 ng/mL 11.8 ± 0.7%, 10 ng/mL 21.5 ± 0.7% and 1 ng/mL 11.3±0.7%). We conclude that growth hormone is effective in promoting the longevity in vitro of ram spermatozoa stored at room temperature, and that this effect is concentration dependent. (1)Breier et al. (1996). Endocrinology 137, 4061–4064.(2)Sauerwein et al. (2000). Dom. Anim. Endocrinol. 18, 145–158.(3)Champion et al. (2002). Theriogenology 57, 1793–1800.
45
Hrymak, K. "Cryopreservation of ram-sires semen with usage of synthetic extenders." Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies 22, no. 92 (May 8, 2020): 62–70. http://dx.doi.org/10.32718/nvlvet-a9211.
Full textAbstract:
The retrospective analysis of the invention of synthetic extender for ram’s semen cryopreservation, developed by different authors, is presented in the article. It has been shown, that in order to ensure the biological usefulness of the extenders for semen, they should contain phospholipids, electrolytes, non-electrolytes, cryoprotectants, biologically active substances and other ingredients, due to the many physical and chemical processes that occur in the process of cooling and deep freezing of spermatozoa. Researchers have established the protective role of phospholipids from chicken egg in protecting sperm from cold stroke. It has been proven that the usage of dried yolk and egg powder in extenders during the cryopreservation of sperm provides a high protective effect of sperm at the level of natural yolk. The degree of effectiveness of different electrolytes in preventing cold shock has been indicated. Their protective function not only for the creation of buffer properties of extender, but also for the reduction of freezing point of aqueous solutions has been emphasized. The importance of sugars in the composition of ram’s semen cryopreservation extenders has been proven. They reduce the conductivity of semen, thereby reducing sperm agglutination, act as antioxidants, inactivate spermolysins, increase the viscosity, structure water, prevent excessive hydration of proteins and organelles are used by sperm for respiration and glycolysis. It has been experimentally proven by a number of researchers, who studied the protective effect of antioxidants for the development of synthetic extender, that the process of cryopreservation intensifies the lipids peroxidation, which disrupts the orderliness of the sperm membranes structure and thereby the oxidation products damage the plasma membrane and adversely affect on the energy of sperm. A perspective direction in the development of new extenders for animal reproduction biotechnology has been highlighted, both for in vitro fertilization of oocytes and for freezing and thawing of semen using the metal nanoparticles.
46
Ali, Ali J. "Effect of Ivermectin on semen parameters and levels of Alanine aminotransferase (ALT) and Aspartate aminotransferase (AST) enzymes in seminal plasma of Iraqi Awassi rams." Al-Qadisiyah Journal of Veterinary Medicine Sciences 13, no. 1 (June 30, 2014): 115. http://dx.doi.org/10.29079/vol13iss1art290.
Full textAbstract:
The objective of this study was to evaluate the effect of Ivermectin on semen quality and estimation of alanine aminotransferase (ALT), and aspartate aminotransferase (AST) in seminal plasma. Six mature Awassi rams aged 2-3 years and weighing 38-42 Kg were used in this study. Semen collection was done weekly for five weeks from the same ram. The first two collection considered as training for the animal and discarded, the second two collection were considers for the control group. After administration of the drug (Ivermectin), semen samples were collected from the Awassi ram 1, 7,14 days. The parameter studies were included semen volume, percentage motility of sperm, sperm concentration, live sperm, and morphology percentage. Samples of seminal plasma were analyzed for the estimation of alanine aminotransferase (ALT), aspartate aminotransferase (AST). Results of this study indicate that Ivermectin leads to significant (P<0.05) decreased in semen parameters after the first semen collection. Reaction time significantly higher (P<0.05) in the treated group after 1 day of injection. Alanine aminotransferase ALT, Aspartate aminotransferase AST show no significant differences in activities in seminal plasma during the study. In conclusion: it is preferable to use the animals for the purpose of reproduction only after at least 7 days after Ivermectin injection due to it harmful effect on semen quality.
47
Haresign, W., S. R. Read, R. M. Curnock, and H. C. B. Reed. "A note on the use of laparoscopy for intrauterine insemination of frozen-thawed semen in the ewe." Animal Science 43, no. 3 (December 1986): 553–56. http://dx.doi.org/10.1017/s0003356100002762.
Full textAbstract:
One of the major limitations to the widespread use of artificial insemination (AI) in the United Kingdom (UK) sheep industry is that with frozen-thawed semen current insemination techniques result in lowered fertility. Consequently, only fresh or liquid-chilled ram semen can be used if commercially acceptable conception rates are to be achieved.
48
Inanc, ME, S. Gungor, C. Ozturk, F. Korkmaz, I. Bastan, and B. Cil. "Cholesterol-loaded cyclodextrin plus trehalose improves quality of frozen-thawed ram sperm." Veterinární Medicína 64, No. 03 (March 20, 2019): 118–24. http://dx.doi.org/10.17221/146/2018-vetmed.
Full textAbstract:
The objective of this study was to determine effects of supplementing Tris-based semen extenders with either cholesterol-loaded cyclodextrin (CLC) or 7-dehydrocholesterol loaded cyclodextrin (7-DCLC) plus trehalose (T) for cryopreservation of ram semen. Semen was collected with an artificial vagina from five Merino rams (2–3 years of age) during the non-breeding season. Ejaculates were pooled, divided into eight equal portions, diluted with a standard Tris-based extender containing: no additive (control); T (50 mM); or T (50 mM) + 1.5, 2.5 or 3.5 mg of either 7-DCLC or CLC. Semen was chilled from 37°C to 4°C, placed in 0.25 ml French straws, held 5 cm above liquid nitrogen for 12 minutes, then plunged into liquid nitrogen. After thawing, a computer-aided semen analyzer system (CASA) was used to assess motility, whereas plasma membrane and acrosome integrity (PMAI) and high mitochondrial membrane potential (HMMP) were assessed with flow cytometry. Sperm supplemented with 2.5 mg and 3.5 mg CLC + T had the highest (P < 0.05) total and progressive motility (65.2 ± 4.7 and 19.0 ± 1.0% respectively, mean ± SEM), albeit with no significant differences from sperm with 1.5 or 3.5 mg CLC + T. Sperm with 2.5 mg CLC + T had the highest (P < 0.05) PMAI (59.3%; not different from 3.5 mg CLC + T) and highest (P < 0.05) HMMP (64.6%; not different from 1.5 or 3.5 mg CLC + T). The lowest ALH value, 2.8 ± 0.3 µm was in the 2.5 mg 7-DCLC + T group; otherwise, there were no significant differences among groups for any other CASA end point. In conclusion, adding CLC + T to a tris-based extender optimized quality of frozen-thawed ram semen. Therefore, extenders including CLC + T have potential to improve quality of frozen-thawed ram sperm.
49
Oláh, J., S. Kusza, S. Harangi, J. Posta, A. Kovács, A. Pécsi, C. Budai, and A. Jávor. "Seasonal changes in scrotal circumference, the quantity and quality of ram semen in Hungary." Archives Animal Breeding 56, no. 1 (October 10, 2013): 102–8. http://dx.doi.org/10.7482/0003-9438-56-010.
Full textAbstract:
Abstract. In this study, the quantitative and qualitative traits of semen were studied in seven rams of different breeds (Prolific Merino, Cokanski Tsigai, Barbados Blackbelly, Bábolna Tetra, Awassi, Ile de France and Suffolk), bred in Hungary. The semen parameters (density, volume, pH, mass motility, % motility, thawing and heat resistance), freezability of semen and the factors influencing these parameters were evaluated with respect to breed and season. The fresh and post-thawing quality of semen varied greatly with the breed and the season. The postthawing motility of semen cells was outstandingly high for Awassi rams in three seasons. During the test period, the smallest scrotal circumference was measured for Barbados Blackbelly, except for the summer when it increased by 12.5 cm. The reintroduction of artificial insemination could lead to a significant advancement of the sheep sector in Hungary. To promote this, we have provided useful and new information for breeders and organisations.
50
Wade, C., and M. Goddard. "How much is a genetically superior ram worth?" Australian Journal of Agricultural Research 45, no. 2 (1994): 403. http://dx.doi.org/10.1071/ar9940403.
Full textAbstract:
The economic value of a genetic difference between rams was estimated for stud and commercial tiers in the Australian Merino industry. Discounted returns were predicted using the method of Hill (1974). Results were generated for breeding schemes considering natural mating (Scheme A), artificial insemination (Scheme B), the use of home-bred sires at daughter stud level (Scheme C) and the use of culled stud ewes in lower tiers (Scheme D). The effects of discount rate and ram flock life were assessed. The value of one extra unit of genetic merit in three traits (Clean Fleece Weight, Fibre Diameter and WOOLPLAN index score) was found for commercial, daughter and parent tiers. One extra kilogram of clean fleece weight (CFW) was worth around $817 for a commercial level ram (all Schemes) and up to $390000 for a parent level ram in Scheme B. The same improvement was worth $4 per semen dose used at commercial level, and up to $3977 at parent stud level (Scheme B). Increasing the discount rate devalued later returns, reducing the number of discounted expressions. Increasing ram flock life increased ram value but reduced semen value. The results are discussed for current industry practice and values obtained previously for commercial rams. The economic value of multiple ovulation and embryo transfer is considered.