Academic literature on the topic 'Randomly Amplified Polymorphic DNA (RAPD)'

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Journal articles on the topic "Randomly Amplified Polymorphic DNA (RAPD)"

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Okatani, Alexandre Tomomitsu, Hideki Hayashidani, Toshio Takahashi, Takahide Taniguchi, Masuo Ogawa, and Ken-ichi Kaneko. "Randomly Amplified Polymorphic DNA Analysis ofErysipelothrix spp." Journal of Clinical Microbiology 38, no. 12 (2000): 4332–36. http://dx.doi.org/10.1128/jcm.38.12.4332-4336.2000.

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The usefulness of randomly amplified polymorphic DNA method (RAPD) to identify each species of genus Erysipelothrix and for epidemiological analysis of this genus was studied. Eighty-one strains and 18 random primers were tested. Among the tested primers, the primers NK51 (GGTGGTGGTATC) and NK6 (CCCGCGCCCC) produced noticeable results. The primer NK51 revealed four species-specific RAPD patterns. Of the 66 strains of E. rhusiopathiae, 64 had the same unique band of 884 bp. Of the 12 strains of E. tonsillarum, 11 produced a 1,265-bp band. In addition, two strains, previously thought to be E. rh
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Wong, N. A. C. S., C. J. Linton, H. Jalal, and M. R. Millar. "Randomly amplified polymorphic DNA typing: a useful tool for rapid epidemiological typing ofKlebsiella pneumoniae." Epidemiology and Infection 113, no. 3 (December 1994): 445–54. http://dx.doi.org/10.1017/s095026880006845x.

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SUMMARYDiscriminatory typing methods are invaluable in the investigation of outbreaks of infectious diseases. Single primers were used to generate randomly amplified polymorphic DNA (RAPD) profiles fromKlebsiella pneumoniaeisolates of various serotype andK. pneumoniaeisolates from cases of sepsis at a Malaysian hospital and two English hospitals. RAPD profiles of acceptable reproducibility, a maximum of three minor band variations, were produced using a rapid DNA extraction method. RAPD typing ofK. pneumoniaewas shown to be as discriminatory as restriction fragment length polymorphism analysis
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Möller, M., and R. Harling. "Randomly amplified polymorphic DNA (RAPD) profiling of Plasmodiophora brassicae." Letters in Applied Microbiology 22, no. 1 (January 1996): 70–75. http://dx.doi.org/10.1111/j.1472-765x.1996.tb01111.x.

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Echt, C. S., L. A. Erdahl, and T. J. McCoy. "Genetic segregation of random amplified polymorphic DNA in diploid cultivated alfalfa." Genome 35, no. 1 (February 1, 1992): 84–87. http://dx.doi.org/10.1139/g92-014.

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Polymerase chain reaction was used, with single 10-mer primers of arbitrary sequence, to amplify random regions of genomic DNA from a diploid cultivated alfalfa backcross population. Segregation of the random amplified polymorphic DNA (RAPD) fragments was analysed to determine if RAPD markers are suitable for use as genetic markers. Of the 19 primers tested, 13 amplified a total of 37 polymorphic fragments, of which 28 (76%) segregated as dominant Mendelian traits. RAPD markers appear useful for the rapid development of genetic information in species like alfalfa where little information curre
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Weir, B. J., R. G. St Pierre, and R. N. Chibbar. "RAPD Marker Polymorphism among Saskatoon Cultivars, Clones, and Seedlings." HortScience 32, no. 6 (October 1997): 1109–13. http://dx.doi.org/10.21273/hortsci.32.6.1109.

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Randomly amplified polymorphic DNA (RAPD) markers were used to distinguish among 16 cultivars of saskatoon (Amelanchier spp.). Eight 9-base, oligonucleotide primers amplified a total of 98 DNA fragments, of which 29 were useful as reproducible polymorphic markers. Twelve cultivars and two pairs of cultivars were uniquely characterized by these 29 markers. Polymorphism was not detected among five sources of the cv. Thiessen, whereas variability was found among seedlings from self-pollinated `Thiessen'. Samples of the cvs. Regent and Parkhill were indistinguishable from one of two sources, sugge
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Lim, Hak-Tae. "Comparative Studies on Hanabusaya asiatica and Its Allied Groups Based on Randomly Amplified Polymorphic DNA (RAPD) Analysis." HortScience 32, no. 3 (June 1997): 482E—482. http://dx.doi.org/10.21273/hortsci.32.3.482e.

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The phylogenetic relationships between Korean endemic, Hanabusaya asiatica, and its allied groups, including four genera and nine species, were investigated at the DNA level using randomly amplified polymorphic DNA (RAPD) method. Ten primers out of 80 primers (10-mer) screened gave rise to very high polymorphism (99%) in all of the tested plants, producing 153 randomly amplified DNA fragments. H. asiatica was differentiated from its allied groups at the 0.62 of similarity index of RAPDs. This results were in accordance with previous classification based on palynological studies. It was confirm
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Picton, Deric D., and Harrison G. Hughes. "Characterization of Alstroemeria Species using Random Amplified Polymorphic DNA (RAPD) Analysis." HortScience 32, no. 3 (June 1997): 482F—482. http://dx.doi.org/10.21273/hortsci.32.3.482f.

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In this study, 11 species, hybrids, and color variants were characterized using randomly amplified polymorphic DNA (RAPD) analysis. Total genomic DNA was extracted using a 2% CTAB extraction buffer using fresh or frozen leaf material. The DNA was amplified using standard RAPD-PCR protocols utilizing 10-mer primers. All primers utilized exhibited a high degree of polymorphism in their banding patterns among the species and hybrids studied. The primers used produced ≈40 reproducible bands. It was possible to identify and uniquely distinguish all species and hybrids investigated using these bands
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Ronning, C. M., R. J. Schnell, and S. Gazit. "Using Randomly Amplified Polymorphic DNA (RAPD) Markers to Identify Annona Cultivars." Journal of the American Society for Horticultural Science 120, no. 5 (September 1995): 726–29. http://dx.doi.org/10.21273/jashs.120.5.726.

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The native American genus Annona contains many species that are cultivated for their edible fruit, including the custard apple (A. reticuluta L.), soursop (A. muricata L.), cherimoya (A. cherimola L.), sugar apple (A. squamosa L.), and interspecific hybrids, the atemoyas. RAPD analysis of A. cherimola. `Campa' and `Jete,' A. squamosa `Lessard,' and the atemoyas `Ubranitzki,' `Malali,' and `Kaspi' resulted in very distinctive patterns, indicating that RAPD markers, may be an efficient method of fingerprinting genotypes within and between Annona species. All 15 primers used generated repeatable,
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Franklin, Rima B., Douglas R. Taylor, and Aaron L. Mills. "Characterization of microbial communities using randomly amplified polymorphic DNA (RAPD)." Journal of Microbiological Methods 35, no. 3 (April 1999): 225–35. http://dx.doi.org/10.1016/s0167-7012(99)00003-2.

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Gidoni, D., M. Rom, T. Kunik, M. Zur, E. Izsak, S. Izhar, and N. Firon. "Strawberry-cultivar Identification using Randomly Amplified Polymorphic DNA (RAPD) Markers." Plant Breeding 113, no. 4 (December 1994): 339–42. http://dx.doi.org/10.1111/j.1439-0523.1994.tb00747.x.

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Dissertations / Theses on the topic "Randomly Amplified Polymorphic DNA (RAPD)"

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Golembiewski, Robert Craig. "Identification and characterization of creeping bentgrass using randomly amplified polymorphic DNA (RAPD) markers /." The Ohio State University, 1997. http://rave.ohiolink.edu/etdc/view?acc_num=osu1487942739809001.

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Woodburn, Mary Alice. "Random amplified polymorphic DNA (RAPD) analysis of Bacillus sphaericus." Thesis, This resource online, 1994. http://scholar.lib.vt.edu/theses/available/etd-07102009-040429/.

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Robert, Florence. "Typage de "Candida albicans" par Random Amplified Polymorphic DNA (RAPD)." Paris 5, 1992. http://www.theses.fr/1992PA05P198.

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Wei, Ling. "Identification of DNA Markers in Triticum aestivum-Aegilops caudata Additions Lines by Randomly Amplified Polymorphic DNA (RAPD) Technology." DigitalCommons@USU, 1995. https://digitalcommons.usu.edu/etd/4543.

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The objective of this study was to identify DNA markers for each of six added C-genome chromosomes in Triticum aestivum L. cv. 'Alceso'-Aegilops caudata L. addition lines using the randomly amplified polymorphic DNA (RAPD) technique. DNA from Ae. caudata, T. aestivum, amphiploid of T. aestivum X Ae. caudata, and six disomic addition lines of wheat having a pair of Ae. caudata chromosomes was used as the template for the amplification of RAPD markers with a total of 58 random 10-mer oligonucleotide primers. Two primers, OPC-08 and OPJ-16, produced one intense band each from the amphiploid of T.
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Papadopoulos, Sarantos. "Untersuchungen genomischer Veränderungen von Mammakarzinomzellen mittels Random Amplified Polymorphic DNA." Doctoral thesis, Humboldt-Universität zu Berlin, Medizinische Fakultät - Universitätsklinikum Charité, 2001. http://dx.doi.org/10.18452/14631.

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Im ersten Teil der Arbeit wurde die random amplified polymorphic DNA (RAPD) Methode für die Anwendung humaner DNA optimiert, indem die Konzentrationen der einzelnen Agentien und das thermische Profil der PCR-Reaktion verändert wurden. Des weiteren wurde der Einfluss von Polymerasen und PCR-Maschinen auf die RAPD, insbesondere auf die Erweiterung des Spektrums der Amplimere und die Reproduzierbarkeit der Reaktionen untersucht. Unsere Untersuchungen haben gezeigt, dass die RAPD eine sehr robuste und reproduzierbare Methode ist. Im zweiten Teil wurden qualitative und quantitative Unterschiede zwi
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Campos, Lázara Pereira. "Genome relationships among Lotus species based on random amplified polymorphic DNA (RAPD)." Thesis, McGill University, 1992. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=56888.

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The usefulness of RAPDs (Random Amplified Polymorphic DNA) to distinguish among different taxa of Lotus was evaluated. The following species were included: L. corniculatus, L. tenuis, L. alpinus, L. japonicus, and L. uliginosus. Several accessions for each species were studied. Following DNA extraction, amplification reactions were performed in a Hybaid DNA Thermal Cycler, and the product visualized according to a standard procedure. Twenty primers were used for each species/accession. Clear bands and several polymorphisms were obtained for all primers. A phenogram was drawn based on the genet
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Rinaldi, Catherine. "Authentication of the Panax genus plants used in Traditional Chinese Medicine (TCM) using Randomly Amplified Polymorphic DNA (RAPD) analysis." University of Western Australia. Centre for Forensic Science, 2007. http://theses.library.uwa.edu.au/adt-WU2008.0054.

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[Truncated abstract] Traditional medicines are used by millions of people throughout the world as their primary source of medical care. A range of materials are in used traditional medicines including plant and animal parts. Even though the traditional medicine trade is estimated to be worth sixty billion dollars annually the trade remains largely unregulated. Unscrupulous practices by vendors to increase their profit margins such as substituting and adulterating expensive material with cheaper varieties go unchecked. This can be dangerous to consumers because some substitutions involve poison
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Patel, Sushma M. "Ribosomal RNA genes and RAPD for Cryptosporidium species and subspecies discrimination." Thesis, Coventry University, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.389769.

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Hughes, Greta. "Population structure and genetics of the European lobster Homarus gammarus." Thesis, Bangor University, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.341219.

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Góis, Plácia Barreto Prata. "Aplicação do RAPD (Randomly Amplified Polymorphic dna) para tipagem molecular de amostras de Salmonella isoladas de diversas fontes da cidade de Aracaju-SE." Universidade Federal de Sergipe, 2006. https://ri.ufs.br/handle/riufs/3673.

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior<br>The Salmonella infection is a relevant problem in the public health, being one of the most important causes of the world´s enteric pathologies, with 1,3 million ill people, resulting in 600 deaths/year. The Salmonella spp. transmission occurs especially through water consumption and contaminated food. The diagnosis is realized using biochemical, serologic, and molecular tests. The RAPD (Randomly Amplified Polymorphic DNA) test is able to detect the genetic variation present in the isolated Salmonella spp. samples, allowing a mole
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Books on the topic "Randomly Amplified Polymorphic DNA (RAPD)"

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Hilton, Anthony Craig. Randomly amplified polymorphic DNA analysis of salmonella & campylobacter. Birmingham: University of Birmingham, 1996.

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Book chapters on the topic "Randomly Amplified Polymorphic DNA (RAPD)"

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Galvão, Lúcia Maria da Cunha, and Eliane Lages-Silva. "Randomly Amplified Polymorphic DNA (RAPD)." In Springer Protocols Handbooks, 133–47. Totowa, NJ: Humana Press, 2008. http://dx.doi.org/10.1007/978-1-60327-375-6_10.

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Babu, Kantipudi Nirmal, Muliyar Krishna Rajesh, Kukkumgai Samsudeen, Divakaran Minoo, Erinjery Jose Suraby, Kallayan Anupama, and Paul Ritto. "Randomly Amplified Polymorphic DNA (RAPD) and Derived Techniques." In Methods in Molecular Biology, 191–209. Totowa, NJ: Humana Press, 2013. http://dx.doi.org/10.1007/978-1-62703-767-9_10.

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Rafalski, Antoni, Scott Tingey, and John G. K. Williams. "Random amplified polymorphic DNA (RAPD) markers." In Plant Molecular Biology Manual, 423–29. Dordrecht: Springer Netherlands, 1994. http://dx.doi.org/10.1007/978-94-011-0511-8_27.

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Rafalski, Antoni, Scott Tingey, and John G. K. Williams. "Random amplified polymorphic DNA (RAPD) markers." In Plant Molecular Biology, 55–63. Dordrecht: Springer Netherlands, 1993. http://dx.doi.org/10.1007/978-94-017-6951-8_3.

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Babu, Kantipudi Nirmal, Thotten Elampilay Sheeja, Divakaran Minoo, Muliyar Krishna Rajesh, Kukkamgai Samsudeen, Erinjery Jose Suraby, and Illathidath Payatatti Vijesh Kumar. "Random Amplified Polymorphic DNA (RAPD) and Derived Techniques." In Methods in Molecular Biology, 219–47. New York, NY: Springer US, 2020. http://dx.doi.org/10.1007/978-1-0716-0997-2_13.

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Sperisen, Christoph, and Urs Büchler. "Cloning of Random Amplified Polymorphic DNA (RAPD) to Generate Codominant Genetic Markers." In Molecular Tools for Screening Biodiversity, 217–22. Dordrecht: Springer Netherlands, 1998. http://dx.doi.org/10.1007/978-94-009-0019-6_43.

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Al-Khalifah, Nasser S., and A. E. Shanavaskhan. "Molecular Identification of Date Palm Cultivars Using Random Amplified Polymorphic DNA (RAPD) Markers." In Methods in Molecular Biology, 185–96. New York, NY: Springer New York, 2017. http://dx.doi.org/10.1007/978-1-4939-7159-6_16.

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Modi, Arpan, Bhavesh Gajera, Naraynan Subhash, and Nitish Kumar. "Evaluation of Clonal Fidelity of Micropropagated Date Palm by Random Amplified Polymorphic DNA (RAPD)." In Methods in Molecular Biology, 81–89. New York, NY: Springer New York, 2017. http://dx.doi.org/10.1007/978-1-4939-7159-6_8.

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Mergeai, G., I. Vroh Bi, J. P. Baudoin, and P. Du Jardin. "Use of Random Amplified Polymorphic DNA (RAPD) Markers to Assist Wide Hybridization in Cotton." In Biotechnology in Agriculture and Forestry, 121–39. Berlin, Heidelberg: Springer Berlin Heidelberg, 1998. http://dx.doi.org/10.1007/978-3-642-80373-4_8.

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Singh, Kamaleshwar P. "Screening of DNA Methylation Changes by Methylation-Sensitive Random Amplified Polymorphic DNA-Polymerase Chain Reaction (MS-RAPD-PCR)." In Molecular Toxicology Protocols, 71–81. Totowa, NJ: Humana Press, 2014. http://dx.doi.org/10.1007/978-1-62703-739-6_6.

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Conference papers on the topic "Randomly Amplified Polymorphic DNA (RAPD)"

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Eriksson, John, Charlotta Löfström, and Peter Rådström. "Randomly amplified polymorphic DNA (RAPD) typing of Salmonella Senftenberg in animal feed production." In Fifth International Symposium on the Epidemiology and Control of Foodborn Pathogens in Pork. Iowa State University, Digital Press, 2003. http://dx.doi.org/10.31274/safepork-180809-538.

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Liu, Haiying, Guie Wang, Xiangying Meng, and Xiuli Wang. "Genetic Variation in Six Oratosquilla oratoria Populations Revealed by Random Amplified Polymorphic DNA (RAPD) Markers." In 2010 4th International Conference on Bioinformatics and Biomedical Engineering (iCBBE). IEEE, 2010. http://dx.doi.org/10.1109/icbbe.2010.5516354.

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Ifah, Arini Al, Endang Yuniastuti, and Parjanto. "Analysis of breadfruit plant diversity (Artocarpus altilis P.) by random amplified polymorphic DNA (RAPD) in DIY." In THE 8TH ANNUAL BASIC SCIENCE INTERNATIONAL CONFERENCE: Coverage of Basic Sciences toward the World’s Sustainability Challanges. Author(s), 2018. http://dx.doi.org/10.1063/1.5062802.

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Darkazanli, Mohamad, Irina Kiseleva, and Kinan Darkazanli. "Genetic diversity of E. coli O157:H7 isolated from Aleppo River water samples using random amplified polymorphic DNA (RAPD) marker." In PHYSICS, TECHNOLOGIES AND INNOVATION (PTI-2019): Proceedings of the VI International Young Researchers’ Conference. AIP Publishing, 2019. http://dx.doi.org/10.1063/1.5134359.

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Li, Zi-tong, Guang-xin Yuan, Miao Wang, Li-jun Gao, Li-hua Zhang, Ming-cheng Li, and Jia-mu Niu. "Tortoise shell and falsify random amplified polymorphism DNA (RAPD) appraisal method research." In 2015 International Forum on Energy, Environment Science and Materials. Paris, France: Atlantis Press, 2015. http://dx.doi.org/10.2991/ifeesm-15.2015.188.

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