Academic literature on the topic 'RAPD analysis'

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Journal articles on the topic "RAPD analysis"

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Raddová, J., M. Beránek, I. Oukropec, and M. Vachůn. "RAPD Analysis of peaches within Czech National collection." Czech Journal of Genetics and Plant Breeding 39, No. 4 (November 23, 2011): 113–19. http://dx.doi.org/10.17221/3728-cjgpb.

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The Random Amplified Polymorphic DNA (RAPD) technique was used to study the genetic diversity and relationships within the collection of the Czech National Plant Genetic Resources (PGR) of peaches (Prunus persica L.). The aim of the work was to elaborate a dendrogram of genetic similarity and to divide collection into clusters. 46 primers were applied to 6 cultivars differing in the place of origin, the fruit shape, the fruit colour, and in some other morphological characteristics. 12 primers were chosen which gave polymorphic repeatable strong and middle strong bands. They were subsequently used for the RAPD reactions within the whole collection of peaches. The selected RAPD primers distinguished 28 peach cultivars and RAPD data were used to group the accessions analysed. Almonds and peach × almond hybrids were clearly separated in the frame of the whole collection. The grouping corresponded to the botanical system, to the available information about pedigree, and to the cultivars description.  
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Gawel, Nick J., Rory Mellinger, Eric Stout, and R. Sauve. "RAPD Analysis of Acer." HortScience 30, no. 4 (July 1995): 813F—813. http://dx.doi.org/10.21273/hortsci.30.4.813f.

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DNA from 27 Acer species was used for RAPD analysis. A relatively high number of phylogenically informative polymorphisms were detected, as would be expected in intraspecific comparisons. Principle coordinates analysis was used to discern groupings among the species and a RAPD-based phylogeny was constructed. As expected when making comparisons among species, very high levels of polymorphism were found. Cultivars that grouped together in the principle components analysis also grouped together in the phylogenic analysis. Parts of the phylogenic analysis do not agree with morphology-based phylogenies. This may be due to poor correlation between morphological and DNA markers, or perhaps RAPDs may be too discriminatory to be used for interspecies comparisons. The extremely high level of between-species variation coupled with the low level of within-species variation, indicates the potential of DNA-based identification and discrimination of Acer species is high.
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Vlastníková, H., K. Moravcová, and M. Pidra. "The RAPD analysis of several cultivars of grapevine (Vitis viniferaL.) and their clones." Horticultural Science 31, No. 4 (November 25, 2011): 136–39. http://dx.doi.org/10.17221/3807-hortsci.

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Nine identification RAPD markers (Moravcová et al. 2003) were used to distinguish 24 clones and grapevine cultivars. No polymorphism was detected among all the tested clones of Chardonnay, Pinot gris and Zweigeltrebe from Polešovice. Pinot noir, Pinot gris, Pinot blanc and Pinot Meunier were indistinguishable within clones, they also showed the identical RAPD profile within cultivars (except discussed sample No. 26). On the other hand, Auxerrois as a relative to cultivars of Pinot group showed unique patterns and may be classified as a different cultivar. Some irregularities within the cultivars of Pinot family from Oblekovice were also found, several of them gave different results from those expected: Pinot blanc sample 26 has the RAPD profile typical of Chardonnay. A new abnormal RAPD pattern as a marker of typical Chardonnay and Pinot profiles was observed in two cases. While RAPD banding patterns could not distinguish between the known clones, they were useful for distinguishing between phenotypically similar cultivars and for assessing the origins of cultivars thought to have originated as sports.    
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Ozbey, G., Ertas HB, and A. Muz. "Random amplified polymorphic DNA (RAPD) analysis of Ornithobacterium rhinotracheale strains isolated from chickens in Turkey." Veterinární Medicína 50, No. 12 (March 28, 2012): 526–30. http://dx.doi.org/10.17221/5660-vetmed.

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Six field strains of Ornithobacterium rhinotracheale isolated from chickens in Elazig province located in the East of Turkey were typed by serotyping and random amplified polymorphic DNA assay using a random primer (OPG-11). Using the AGP test used for serotyping, serotype A was found to be the predominant serotype, only one strain was serotyped as serotype B. By RAPD assay, the tested ORT strains were found to have different RAPD profiles. In addition, the RAPD assay showed almost similar DNA profiles among the tested strains of the serotypes A, B, D and E. The strain of serotype C did give a different RAPD profile. Within strains of the same serotype (A), different profiles were found but the strain of serotype (B) had an identical profile as strains of serotype A. This study suggests that more genotypes of ORT strains are present within the same serotype and thus that no relationship exists between the RAPD pattern of ORT and their serotype.
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Tigano, Myrian S., and Salah Aljanabi. "RAPD Analysis of Nomuraea rileyi." Journal of Invertebrate Pathology 75, no. 3 (April 2000): 240–42. http://dx.doi.org/10.1006/jipa.1999.4920.

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Säull, T., C. Lind-Halldén, and C. Halldén. "Primer Mixtures in RAPD Analysis." Hereditas 132, no. 3 (May 5, 2004): 203–8. http://dx.doi.org/10.1111/j.1601-5223.2000.00203.x.

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Rasmussen, H. N., J. E. Olsen, and O. F. Rasmussen. "RAPD analysis of Yersinia enterocolitica." Letters in Applied Microbiology 19, no. 5 (November 1994): 359–62. http://dx.doi.org/10.1111/j.1472-765x.1994.tb00475.x.

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Miyata, M., T. Aoki, V. Inglis, T. Yoshida, and M. Endo. "RAPD analysis ofAeromonas salmonicidaandAeromonas hydrophila." Journal of Applied Bacteriology 79, no. 2 (August 1995): 181–85. http://dx.doi.org/10.1111/j.1365-2672.1995.tb00933.x.

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Scott, Michelle Pellissier, Kenneth M. Haymes, and Scott M. Williams. "Parentage analysis using RAPD PCR." Nucleic Acids Research 20, no. 20 (1992): 5493. http://dx.doi.org/10.1093/nar/20.20.5493.

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Baleiras Couto, M. M., J. M. B. M. van der Vossen, H. Hofstra, and J. H. J. Huis in 't Veld. "RAPD analysis: a rapid technique for differentiation of spoilage yeasts." International Journal of Food Microbiology 24, no. 1-2 (December 1994): 249–60. http://dx.doi.org/10.1016/0168-1605(94)90123-6.

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Dissertations / Theses on the topic "RAPD analysis"

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Woodburn, Mary Alice. "Random amplified polymorphic DNA (RAPD) analysis of Bacillus sphaericus." Thesis, This resource online, 1994. http://scholar.lib.vt.edu/theses/available/etd-07102009-040429/.

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Roberts, David Mark. "Genome analysis of plant and insect pathogenic species of Verticillium using molecular DNA methodologies." Thesis, King's College London (University of London), 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.313053.

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Chani, Eduard. "Molecular marker analysis of a segregating monoploid potato family." Diss., Virginia Tech, 1998. http://hdl.handle.net/10919/29792.

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Anther culture experiments were conducted to construct a monoploid family. The donor plants used were hybrids between high leptine producing selections of Solanum chacoense Bitt. and anther culture responsive selections of Solanum phureja Juz. et Buk. Several steps of the anther culture process were studied. The results indicated that genotype remains the main factor affecting anther culture response. Growing anther-donor plants in higher greenhouse temperatures (30 degrees C day/20 degrees C night) increased the number of embryos per anther by 40 percent. A heat shock given to anthers in culture for 12h at 35 degrees C was also found to be beneficial resulting in an increase of the anther culture response by 40 percent. However the effect of the high temperature shock resulted in lower regeneration rates. In all experiments a highly significant "date" effect was observed with one or two days differing from the others by showing higher response rates in all hybrids tested. The majority of the regenerated plants was diploid, probably resulting from unreduced gametes. Simple sequence repeat analysis with eight polymorphic primer pairs was used successfully to identify the homozygous diploid plants that were added to the monoploids. In total 34 monoploid plants and 14 homozugous diploids were obtained. The degree of heterozygosity revealed by SSR analysis indicated that the diploid plants originated from unreduced gametes formed by first division restitution (FDR) mechanism. The SSR marker data were used to map the genes with respect to the centromeres by half tetrad analysis. SSR-containing sequences from the public databases, as well as sequences obtained from a genomic library enriched for SSRs, were used to generate 48 primer pairs. Only 12 of them were found to be polymorphic in the monoploid family. Ten primer pairs did not amplify any specific fragment. The monoploid population showed distorted segregation at four of the polymorphic loci, showing overrepresentation of the chacoense alleles in three of them. One of the loci showing distorted segregation (STSTP, amplified by primer pair RV 11+12) is most probably linked to lethal alleles, whereas another one (ST13ST, amplified by primer pair RV 21+22) could be linked to genes affecting anther-culture response. The location of the SSR loci on the potato chromosomes is not known except for one (waxy, primer pair 3+4), but statistical analysis on the segregation data obtained from 70 heterozygous anther-derived diploids showed no linkage between them. The SSR primer pairs developed in this study might be useful in studying genetic relationships among cultivars and accessions in breeding programs. Randomly amplified polymorphic DNA (RAPD) analysis was used in association with bulked segregant analysis to detect linkage with genes controlling leptine biosynthesis. With all the limitations imposed by the population size and contamination from foreign pollen, a band amplified by primer OPA-16 could differentiate the bulks contrasting for leptine content. It is possible that this band is linked to genes suppressing leptine biosynthesis, since it appears only in the plants that do not synthesize leptines. Further investigation with larger populations is needed to confirm this possibility.
Ph. D.
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Weir, Brian James. "Development and application of RAPD analysis for intra- and interspecific characterization within the genus Amelanchier." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1996. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp05/nq23957.pdf.

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Wamser, Gerson Henrique. "Divergência em genótipos de cebola." Universidade do Estado de Santa Catarina, 2011. http://tede.udesc.br/handle/handle/1125.

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Made available in DSpace on 2016-12-08T16:44:39Z (GMT). No. of bitstreams: 1 PGPV11MA080.pdf: 299920 bytes, checksum: 31fddfd15b77ee0da63e2e67e528bf3f (MD5) Previous issue date: 2011-05-20
Considering that genetic variability is essential for any breeding program, one of the first steps to be taken by the breeder is to determine the genetic variability available. Is this study sought to determine teh divergence between the genotypes studied using predictive techniques, ie, those based on the morphological, physiological or molecular, quantified in a measure of similarity or dissimilarity, wich can express the degree of genetic diversity among the possible parentes. We studied fifteen genotypes used in the state of Santa Catarina: Super superprecoce, Bela Vista, Baia Indaial, Crioula Roxa and Crioula Branca (Populations); Empasc 352 Bola Precoce, Empasc 355 Juporanga, Epagri 362 Crioula Alto Vale and Epagri 363 Superprecoce (comercial populations Epagri) bela Catarina, Bella Vista and Bella Dura (Hybrid s comercial Sakata) Boreal and Gauchinha (Hortec Populations company s business); Catarina (population Agritu comercial company). The first part of the work was divided into two experiments were conducted in experimental stations of Epagri Ituporanga and Lages. The design was a randomized block designs with three replications at each location. Weevaluated the following characteristics: i) length of pseudostem in cm, ii) number of leaves per pseudostem iii) stem diameter in mm; iv) bulb diameter in cm, v) height of the bulb in cm; vi) weight bulb in grams; vii) ratio of height: diameter of the bulb (value obtained by the ratio between height and diameter of the bulb), viii) total production of bulbs in kg há-1, ix) bulkb shape, x) flowering percentage and xi) percentage of rotten bulbs. We performed multivariate analysis of variance and produced a matrix of dissimilarity based on Mahalanobis distance. The variables that contributed most to the divergence were the production of bulbs and total length of the pseudostem to the environment Ituporanga and percentage of flowering and bulb weight of the environment Lages. The second experimente was conducted at the Institute Molecular genetics and Breeding of UDESC IMEGEM. Genotype were planted in the greenhouse, thus, the extraction of DNA from plants are Young. We used eleven primers of 10 bases, which produce thirty and five bands, wirh twenty-eight polymorphic. We used the Jaccard index as a measure of similarity and UPGMA clustering method for the preparation of a dendrogram of genetic similarity. The results showed that there is divergence between the genotypes studied, Crioula Roxa and Bola Precoce the most divergente, with 0.27 similarity and were less divergente Bella Vista and Bella Dura wirh 0.89 similarity
Considerando que a variabilidade genética é essencial para qualquer programa de melhoramento, um dos primeiros passos a ser dado pelo melhorista, é determinar a variabilidade disponível. Neste trabalho procurou-se determinar a divergência existente entre os genótipos estudados através de técnicas preditivas, ou seja, que têm por base as diferenças morfológicas, fisiológicas ou moleculares, quantificadas em uma medida de dissimilaridade ou similaridade, que possa expressar o grau de diversidade genética entre os possíveis genitores. Foram estudados quinze genótipos utilizados no estado de Santa Catarina: Super Superprecoce, Bela Vista, Baia Indaial, Crioula Roxa e Crioula Branca (Populações); Empasc 352 - Bola Precoce, Empasc 355 - Juporanga Epagri 362 Crioula Alto Vale e Epagri 363 - Superprecoce (Populações comerciais da Epagri); Bella Catarina, Bella Vista e Bella Dura (Híbridos comerciais da empresa Sakata); Boreal e Gauchinha (Populações comerciais da empresa Hortec); Catarina (população comercial da empresa Agritu). A primeira parte do trabalho foi dividida em dois experimentos, sendo realizados nas estações experimentais da Epagri de Ituporanga e de Lages. O delineamento utilizado foi de blocos casualizados com três repetições em cada local. Foram avaliadas as seguintes características: i) comprimento do pseudocaule em cm; ii) número de folhas por pseudocaule; iii) diâmetro do pseudocaule em mm; iv) diâmetro do bulbo em cm; v) altura do bulbo em cm; vi) peso do bulbo em gramas; vii) relação altura: diâmetro do bulbo (valor obtido pela razão entre a altura do bulbo e o diâmetro); viii) produção total de bulbos em kg ha-1; ix) forma do bulbo; x) porcentagem de florescimento e xi) porcentagem de bulbos podres. Foi realizada a análise da variância multivariada e elaborada uma matriz de dissimilaridade, com base na distância de Mahalanobis. As variáveis que mais contribuíram para a divergência foram a produção total de bulbos e comprimento do pseudocaule para o ambiente Ituporanga e percentagem de florescimento e peso do bulbo para o ambiente Lages. O segundo experimento foi realizado no Instituto de Melhoramento e Genética Molecular da UDESC IMEGEM. Os genótipos foram semeados em casa de vegetação, sendo realizada a extração de DNA das plantas ainda jovens. Foram utilizados onze oligonucleotídeos iniciadores de 10 bases, que produziram trinta e cinco bandas, sendo vinte e oito polimórficas. Foi utilizado o coeficiente de Jaccard como medida de similaridade e o método de agrupamento UPGMA para a elaboração de um dendrograma de similaridade genética. Os resultados demonstraram que existe divergência entre os genótipos estudados, sendo Crioula Roxa e Bola Precoce os mais divergentes, com 0,27 de similaridade e os menos divergentes foram Bella Vista e Bella Dura, com 0,89 de similaridade
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Rinaldi, Catherine. "Authentication of the Panax genus plants used in Traditional Chinese Medicine (TCM) using Randomly Amplified Polymorphic DNA (RAPD) analysis." University of Western Australia. Centre for Forensic Science, 2007. http://theses.library.uwa.edu.au/adt-WU2008.0054.

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[Truncated abstract] Traditional medicines are used by millions of people throughout the world as their primary source of medical care. A range of materials are in used traditional medicines including plant and animal parts. Even though the traditional medicine trade is estimated to be worth sixty billion dollars annually the trade remains largely unregulated. Unscrupulous practices by vendors to increase their profit margins such as substituting and adulterating expensive material with cheaper varieties go unchecked. This can be dangerous to consumers because some substitutions involve poisonous material. Also, animal parts from endangered species can find their way into traditional medicines, therefore there needs to be a way to identify them in traditional medicines to prosecute poachers. The traditional techniques used for the identification of material used in Traditional Chinese Medicine (TCM) include, morphological, histological, chemical and immunological analysis. However, these techniques have their limitations. This makes applying multiple techniques essential to provide thorough authentication of the material. DNA profiling provides a technique well suited to analysing material used in TCM. DNA profiling is advantageous over other techniques used to authenticate material used in TCM because it requires only a small sample amount, can determine the cultivator, be used on all forms of TCM and potentially distinguish the components of mixtures. ... Therefore, profiles of different species/individual are different and species? can be distinguished. Commercially sold traditional medicines are processed which is likely to degrade the DNA of the sample making extraction and amplification difficult. Here an organic Phenol:Chloroform extraction technique extracted DNA from commercial dried root samples. The extracted DNA was amplifiable using RAPD primers. The RAPD primers used here produced enough polymorphic bands to distinguish different plant species. They were used to distinguish commercial samples that were sold as three different species within the Panax genus, Panax ginseng, Panax quinquefolium and Panax notoginseng and genetically unrelated plant material; Potato and Eleutherococcus senticosus. Liquid samples and mixtures were also profiled with the RAPD primers to determine whether the RAPD primers provide enough distinguishing ability to analyse these forms of TCM. DNA was extracted from the liquid samples, one a ginseng drink and the other an ginseng extractum. However, there was no reliability in the production of PCR products. The analysis of the mixture samples found that not enough polymorphic bands were produced by the RAPD primers used here to identify Panax species within mixtures of two Panax species. While when P. ginseng was mixed with a genetically unrelated sample there was enough polymorphism to differentiate the two samples in the mixture. The results of this research show that RAPD analysis provides a simple and inexpensive technique to begin analysis of materials used in TCM. Using RAPD analysis it is possible to distinguish Panax plant species from each other. However, the RAPD primers used here did not provide enough reproducibility or polymorphism to analyse liquid and mixtures of Panax species plants.
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Paterson, Ian. "Molecular genetic (RAPD) analysis of Leach's storm petrels (Oceanodroma leucorhoa) from three breeding islands in Atlantic Canada." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp05/mq22034.pdf.

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Ahmed, Sabina. "Availability of Nitrogen in Rice-Azolla Dual System and Phylogeny Analysis of Genus Azolla Using RAPD Markers." Kyoto University, 2009. http://hdl.handle.net/2433/124004.

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Kyoto University (京都大学)
0048
新制・課程博士
博士(農学)
甲第14692号
農博第1774号
新制||農||971(附属図書館)
学位論文||H21||N4465(農学部図書室)
UT51-2009-D404
京都大学大学院農学研究科農学専攻
(主査)教授 谷坂 隆俊, 教授 稲村 達也, 教授 冨永 達
学位規則第4条第1項該当
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Alamri, Sarah. "COMPARATIVE ANALYSIS OF SOYBEAN (GLYCINE MAX) ACCESSIONS USING INTER SIMPLE SEQUENCE REPEAT (ISSR) AND RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) MARKERS." Thesis, Laurentian University of Sudbury, 2014. https://zone.biblio.laurentian.ca/dspace/handle/10219/2201.

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Soybean (Glycine max) is an important crop in the world in terms of total production and usage. It is also among the least diverse species. The main objectives of the present study were 1) to determine differences between ISSR and RAPD marker systems in detecting genetic variation in soybeans and 2) to identify and characterize accession- diagnostic molecular markers in G. max accessions. Genomic DNAs from 108 G. max accessions from 11 different gene pools were analyzed using several ISSR and RAPD primers. The levels of polymorphic loci detected with the two marker systems were in general moderate and similar.. Overall, 82% of genetic distance values were above 0.40 based on ISSR analysis. However, RAPD data revealed that the accessions from different countries are closely related with 64% genetic distance values below 0.40. The dendrograms constructed with ISSR data revealed that the South Korean accessions formed an out-group while the RAPD analysis showed that accessions from Sweden were separate from the other 10 gene pools. One variety-diagnostic marker generated with ISSR 5 primer was identified in the accession Kao Chien Tao from China. This marker was cloned, and sequenced. Although RAPD and ISSR marker systems detected similar levels of genetic variability, they target different regions of the soybean genome, resulting in different clustering of the 11 gene pools indicating different genetic relatedness among them. This finding demonstrates the usefulness of both marker systems in assessing diversity and relatedness among Glycine max gene pools.
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陳堅峰 and Kin-fung Chan. "Phylogenetic relationships and genetic diversity detected by rapd and isozyme analysis of crop and weedy species of amaranthus." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 1996. http://hub.hku.hk/bib/B29803846.

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Books on the topic "RAPD analysis"

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Kisuke, Connie. Rape: A critical analysis. Nairobi: Uzima, 2008.

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Zmuda, Joseph. The quick analysis: Rapid handwriting-analysis techniques. San Francisco, CA: Z-Grafic Publications, 1986.

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Yu, Chuck Wah (Francis). Solution thermochemistry for rapid analysis. Salford: University of Salford, 1987.

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Veeraraghavan, Vimala. Rape and victims of rape: A socio-psychological analysis. New Delhi: Northern Book Centre, 1987.

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A, Clark Stuart, ed. Rapid detection assays for food and water. Cambridge: Royal Society of Chemistry, 2001.

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Bourhy, H. Rapid rabies enzyme immunodiagnosis kit. Geneva: World Health Organization, 1988.

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Patel, P. D. Rapid Analysis Techniques in Food Microbiology. Boston, MA: Springer US, 1995.

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Driver, Christopher M. E. Rapid prototype models for optical analysis. [s.l.]: typescript, 2000.

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Wright, Alan A. Analysis of glucosinolates in oilseed rape. Wolverhampton: University of Wolverhampton, 1995.

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Patel, P. D., ed. Rapid Analysis Techniques in Food Microbiology. Boston, MA: Springer US, 1995. http://dx.doi.org/10.1007/978-1-4615-2662-9.

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Book chapters on the topic "RAPD analysis"

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Tingey, Scott V., J. Antoni Rafalski, and Michael K. Hanafey. "Genetic Analysis with RAPD Markers." In Plant Molecular Biology, 491–500. Berlin, Heidelberg: Springer Berlin Heidelberg, 1994. http://dx.doi.org/10.1007/978-3-642-78852-9_45.

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Taylor, P. W. J., T. A. Fraser, H. L. Ko, and R. J. Henry. "RAPD Analysis of Sugarcane During Tissue Culture." In Current Issues in Plant Molecular and Cellular Biology, 241–46. Dordrecht: Springer Netherlands, 1995. http://dx.doi.org/10.1007/978-94-011-0307-7_32.

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Fujimori, F., H. Takaya, Y. Anzai, and T. Okuda. "RAPD and PAUP Analysis for Microbial Screening Programs." In Fingerprinting Methods Based on Arbitrarily Primed PCR, 227–41. Berlin, Heidelberg: Springer Berlin Heidelberg, 1997. http://dx.doi.org/10.1007/978-3-642-60441-6_25.

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Saunders, G. C., and S. J. Owens. "RAPD and ITS Analysis of Orchid Mycorrhizal Fungi." In Mycorrhiza Manual, 413–24. Berlin, Heidelberg: Springer Berlin Heidelberg, 1998. http://dx.doi.org/10.1007/978-3-642-60268-9_26.

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Bin, Wang, Li Songtao, Zhang Zhongting, Bao Xiaoming, and Huang Baiqu. "Identification of Wheat-Wheatgrass Translocation Lines by RAPD Analysis." In Biotechnology in Agriculture, 240–44. Dordrecht: Springer Netherlands, 1993. http://dx.doi.org/10.1007/978-94-011-1779-1_39.

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Graham, G. C., and R. J. Henry. "Preparation of Fungal Genomic DNA for PCR and RAPD Analysis." In Fingerprinting Methods Based on Arbitrarily Primed PCR, 29–34. Berlin, Heidelberg: Springer Berlin Heidelberg, 1997. http://dx.doi.org/10.1007/978-3-642-60441-6_5.

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Mikhailova, N., and K. Johannesson. "A comparison of different protocols for RAPD analysis of Littorina." In Aspects of Littorinid Biology, 33–42. Dordrecht: Springer Netherlands, 1998. http://dx.doi.org/10.1007/978-94-011-5336-2_5.

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Waugh, R. "RAPD Analysis: Use for Genome Characterization, Tagging Traits and Mapping." In Plant Molecular Biology — A Laboratory Manual, 305–33. Berlin, Heidelberg: Springer Berlin Heidelberg, 1997. http://dx.doi.org/10.1007/978-3-642-87873-2_6.

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Black, William C., and Nancy M. DuTeau. "RAPD-PCR and SSCP analysis for insect population genetic studies." In The Molecular Biology of Insect Disease Vectors, 361–73. Dordrecht: Springer Netherlands, 1997. http://dx.doi.org/10.1007/978-94-009-1535-0_31.

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Helliot, B., D. Madur, M. Brison, E. Dirlewanger, and Mt De Boucaud. "Genetic Stability Analysis of Cryopreserved Prunus Ferlenain Rootstock by RAPD and AFLP." In Plant Biotechnology and In Vitro Biology in the 21st Century, 249–50. Dordrecht: Springer Netherlands, 1999. http://dx.doi.org/10.1007/978-94-011-4661-6_58.

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Conference papers on the topic "RAPD analysis"

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Aputri, Farah Nuriessa, Laila Hanum, Rahmat Pratama, and Yuanita Windusari. "Analysis of Mosquito Genetic with PCR-RAPD Approach." In 2021 IEEE International Conference on Health, Instrumentation & Measurement, and Natural Sciences (InHeNce). IEEE, 2021. http://dx.doi.org/10.1109/inhence52833.2021.9537231.

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"Molecular analysis of sugar beet samples using the RAPD method." In Plant Genetics, Genomics, Bioinformatics, and Biotechnology. Novosibirsk ICG SB RAS 2021, 2021. http://dx.doi.org/10.18699/plantgen2021-001.

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Besedina, E. N., V. I. Kil, and M. N. Karpunin. "UNIVERSAL RAPD AND ISSR PRIMERS FOR LACE BUGS PCR ANALYSIS (HETEROPTERA: TINGIDAE)." In STATE AND DEVELOPMENT PROSPECTS OF AGRIBUSINESS. DSTU-PRINT, 2020. http://dx.doi.org/10.23947/interagro.2020.1.393-396.

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46 RAPD and 25 ISSR primers were tested for universality and specificity for DNA of bugs of the lace family (Heteroptera, Tingidae). Universal RAPD primers (OPA 07, 09, 18), with high specificity and revealing genetic polymorphism in lace bugs were revealed. These primers can be used for intraspecific and interspecific comparisons of the studied bugs
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Aloysius, Suyitno, Ixora S. Mercuriani, Ratnawati, and Sudarsono. "Molecular Characterization of Orchid Variants Spathoglottis plicata Blume Based on RAPD Analysis." In 7th International Conference on Research, Implementation, and Education of Mathematics and Sciences (ICRIEMS 2020). Paris, France: Atlantis Press, 2021. http://dx.doi.org/10.2991/assehr.k.210305.009.

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Auvira, Fay Della Prika, Ixora Sartika Mercuriani, and Suyitno Aloysius. "Genetic Variability Analysis of Terrestrial Spathoglottis plicata Orchid Variants Based on RAPD Marker." In 7th International Conference on Research, Implementation, and Education of Mathematics and Sciences (ICRIEMS 2020). Paris, France: Atlantis Press, 2021. http://dx.doi.org/10.2991/assehr.k.210305.011.

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Ifah, Arini Al, Endang Yuniastuti, and Parjanto. "Analysis of breadfruit plant diversity (Artocarpus altilis P.) by random amplified polymorphic DNA (RAPD) in DIY." In THE 8TH ANNUAL BASIC SCIENCE INTERNATIONAL CONFERENCE: Coverage of Basic Sciences toward the World’s Sustainability Challanges. Author(s), 2018. http://dx.doi.org/10.1063/1.5062802.

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Permatasari, Fitria, Ixora S. Mercuriani, and Evy Yulianti. "Genetic similarity analysis of Rhynchostylis retusa (L.) Blume orchids using OPA 15 and OPA 03 RAPD marker." In THE 8TH ANNUAL BASIC SCIENCE INTERNATIONAL CONFERENCE: Coverage of Basic Sciences toward the World’s Sustainability Challanges. Author(s), 2018. http://dx.doi.org/10.1063/1.5062755.

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STAPULIONYTĖ, Asta, Skaistė BONDZINSKAITĖ, Monika STRAVINSKAITĖ, Raimondas ŠIUKŠTA, Ričardas TARAŠKEVIČIUS, and Tatjana ČĖSNIENĖ. "SOIL GENOTOXICITY BIOMONITORING IN RECULTIVATED FACTORY AREA USING THE CYTOGENETIC AND MOLECULAR ASSAYS IN TWO PLANT TEST-SYSTEMS." In RURAL DEVELOPMENT. Aleksandras Stulginskis University, 2018. http://dx.doi.org/10.15544/rd.2017.025.

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Soil pollution with industrial leftovers is of real danger to living organisms since harmful effects can arise after exposure to the contaminants in the soil. In our study, we applied a plant bioassay battery to monitor soil genotoxicity after short-term exposure to the soil. The soil was collected in 3 rounds: at the central part of the brownfield before (S-I) and after (S-III) topsoil removal, and at the brownfield periphery (S-II). The permissible value of the total contamination index is <16 and the corresponding values were 780 in S-I, 69 in S-II and 133 in S-III soil showing that whole brownfield territory is extremely polluted with heavy metals. Cytogenetic markers were recorded in Allium and Tradescantia test-systems and two types of molecular markers, RAPD and ISSR, were analysed in Allium. Our results revealed that the most polluted soil sample has induced an alarming increase of apoptotic cells in onion roots. Chromosome aberration and micronuclei frequency in Allium decreased inconsistently along with the pollution reduction in the soil. Increased frequencies of all cytogenetic markers were revealed in Tradescantia cuttings after exposure to the S-I soil extracts. Cluster analysis of Allium RAPD and ISSR markers showed that the most polluted soil samples induced genetic changes in onions different from those induced by the least polluted soil. Both plant test-systems in this study confirm that soil from the brownfield is harmful to plants and is potentially hazardous to humans.
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Ciptadi, G., M. Mudawamah, V. M. A. Nurgiartiningsih, S. Wahjuningsih, Rr F. D. Listiani, Susiati, L. Hakim, and A. Budiarto. "Reproduction performance and phenogram analysis of local swamp buffalo in East Java with a case of inbreeding based on phenotypic and DNA-RAPD characteristics." In THE 8TH ANNUAL BASIC SCIENCE INTERNATIONAL CONFERENCE: Coverage of Basic Sciences toward the World’s Sustainability Challanges. Author(s), 2018. http://dx.doi.org/10.1063/1.5062807.

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Chelu, Cristina, Carmen Varlam, Gheorghe Titescu, and Gallia Butnaru. "Diversitatea moleculară a două ecotipuri de Datura inoxia provenite din vestul şi estul României." In International Scientific Symposium "Plant Protection – Achievements and Prospects". Institute of Genetics, Physiology and Plant Protection, Republic of Moldova, 2020. http://dx.doi.org/10.53040/9789975347204.03.

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Molecular Diversity of two Ecotypes of Datura inoxia Originating from Western and Eastern Romania. To characterize genomic variation among genotypes, we have performed RAPD analysis using ten random primers. The results yielded 88 bands out of which 39 were polymorphic. The primers US1 and US7 showed 87.71% and 72.72% polymorphism respectively. The least polymorphism was shown by primer US9 (12.50%). The primer US15 did not produce any bands suggesting the absence of matching sequences in the genomic DNA. The dendrogram classified ecotypes into two clusters (A and B); cluster B possess three sub-clusters: B1 - Socodor 2; B2 - Flamura 1 and Flamura 2, and B3 - Flamura 3. Overall, the values of genetic similarity between ecotypes were low pointing out their particular origin and “evolution”.
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Reports on the topic "RAPD analysis"

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Amzeri, Achmad, Kaswan Badami, and Gita Pawana. Inheritance of resistance to downy mildew (Peronosclerospora maydis) in crossing of Madura Maize Plant (Zea mays L.). Innovative Scientific Information & Services Network, May 2019. http://dx.doi.org/10.21107/amzeri.2019.1.

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Hybridization of Back cross is one method to get varieties that are resistant to downy mildew. The purpose of this study was to obtain information on inheritance characteristics of downy mildew resistance. This research was conducted at the experiment center of Agro-Technology Study Program of Agriculture Faculty, University of Trunojoyo Madura. Research of Assessment of resistance to Downy Mildew used a randomized block design with 18 treatments (P1, P2, F1, F2, BC1P1 and BC1P2 in three sets of crosses, namely LGL x Mdr-3, T12 x Mdr-1 and E02 x Mdr-2) and three replications so there were 54 experimental units. Identification of polymorphic RAPD markers for endurance to downy mildew through Bulk Segregant Analysis (BSA) was done by amplifying the DNA in the resistant pool and susceptible pool. The random primers used were 120 primers from 6 operon groups, namely OPA, OPB, OPC, OPD, OPF and OPG. The results showed that the inheritance pattern of maize genetic resistance to downy mildew followed a segregation pattern of 3:1 with a degree of dominance between -1 and 0, and was controlled by incomplete partially negative dominant gene. OPC-07 was a marker that was linkage close to the resistance to downy mildew with a genetic distance of 1.9 cM.
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Berney IV, Ernest S., and Ronald E. Wahl. A Rapid Soils Analysis Kit. Fort Belvoir, VA: Defense Technical Information Center, March 2008. http://dx.doi.org/10.21236/ada483829.

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Kidder, Thalia, Carine Pionetti, Unity Chipfupa, and Jane Remme. Participatory Methodology: Rapid Care Analysis. Oxfam, November 2016. http://dx.doi.org/10.21201/2016.620147.

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Chipfupa, Unity, Thalia Kidder, Imogen Davies, and Jane Remme. Rapid Care Analysis Training Modules. Oxfam, March 2018. http://dx.doi.org/10.21201/2017.1862.

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Coleman, Sarah, Linda Lopez, and David Luntz. An Analysis of Army Rapid Acquisition. Fort Belvoir, VA: Defense Technical Information Center, September 2015. http://dx.doi.org/10.21236/ad1008892.

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Buttler, D., D. Andrzejewski, K. Stevens, D. Anastasiu, and B. Gao. Rapid Exploitation and Analysis of Documents. Office of Scientific and Technical Information (OSTI), November 2011. http://dx.doi.org/10.2172/1033748.

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Smullen, Daniel, and Travis Breaux. Towards Rapid Recertification Using Formal Analysis. Fort Belvoir, VA: Defense Technical Information Center, April 2015. http://dx.doi.org/10.21236/ada623140.

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Toma, Iulia. Rapid Care Analysis in a Rapid-Onset Emergency: Cox’s Bazar, Bangladesh. Oxfam, June 2018. http://dx.doi.org/10.21201/2018.2777.

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Ghormley, Douglas, Stephen Jones, Denis Bueno, Michelle Leger, Timothy Loffredo, and Geoffrey Reedy. RAMSeS: Rapid Analysis of Mission Software Systems. Office of Scientific and Technical Information (OSTI), December 2020. http://dx.doi.org/10.2172/1735977.

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Fink, Eugene, Jaime G. Carbonell, Anatole Gershman, Ganesh Mani, and Dwight Dietrich. Representation and Analysis of Probabilities Intelligence Data (RAPID). Fort Belvoir, VA: Defense Technical Information Center, April 2009. http://dx.doi.org/10.21236/ada498238.

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