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Journal articles on the topic 'Rapd Analysis of P. Aeruginosa'

Author: Grafiati

Published: 3 June 2025

Last updated: 31 July 2025

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1

Auda, Ibtesam Ghadban, Israa M. S. Al-Kadmy, Sawsan Mohammed Kareem, et al. "RAPD- and ERIC-Based Typing of Clinical and Environmental Pseudomonas aeruginosa Isolates." Journal of AOAC INTERNATIONAL 100, no. 2 (2017): 532–36. http://dx.doi.org/10.5740/jaoacint.16-0267.

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Abstract Pseudomonas aeruginosa is a major cause of nosocomial infection in children and adults, resulting in significant morbidity and mortality due to its ability to acquire drug resistance. The ability of P. aeruginosa in the environment to cause infection in individuals has been reported previously; henceforth, surveillance of the emergence and transmission of P. aeruginosa strains among patients is important for infection control in a clinical setup. Various gene-typing methods have been used for epidemiological typing of P. aeruginosa isolates for the purpose of surveillance. In this wor

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Sallman, Ruqaia Sabbar, Suzan Saadi Hussein, and Munum Radwan Ali. "ERIC- PCR Typing, RAPD-PCR Fingerprinting and Quorum Sensing Gene Analysis of Pseudomonas aeruginosa Isolated from Different Clinical Sources." Al-Mustansiriyah Journal of Science 29, no. 2 (2018): 50. http://dx.doi.org/10.23851/mjs.v29i2.345.

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Recently, Pseudomonas aeruginosa infections proportions have increased significantly. Molecular typing and virulence analysis are good techniques, which can lead us to know P. aeruginosa infections. P. aeruginosa isolates were identified by using molecular method (16S rDNA gene) via PCR technique for accurate identification. The highest percent 41.26% of P. aeruginosa bacteria was found in the burn infections followed by 28.57% in wound swabs, 17.46% in ear discharge and lowest percentage were obtained from sputum samples. All isolates classified into six groups (A-F) according to classes of a

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Speijer, Han, Paul H. M. Savelkoul, Marc J. Bonten, Ellen E. Stobberingh, and Jeroen H. T. Tjhie. "Application of Different Genotyping Methods forPseudomonas aeruginosa in a Setting of Endemicity in an Intensive Care Unit." Journal of Clinical Microbiology 37, no. 11 (1999): 3654–61. http://dx.doi.org/10.1128/jcm.37.11.3654-3661.1999.

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Colonization with Pseudomonas aeruginosa was studied by taking serial swab specimens from the oropharynges and anuses and tracheal and gastric aspirates from patients in an intensive care unit during a 10-month period in a setting of endemicity. Nineteen (10%) of the 192 patients included in the study were colonized on admission, while another 30 (16%) patients acquired P. aeruginosawhile in the hospital. Typing of 353 isolates was performed by random amplified polymorphic DNA (RAPD) analysis, and 56 strains were selected for further typing by RAPD analysis, pulsed-field gel electrophoresis (P

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Ravenni, N., P. Cocchi, S. Campana, C. Braggion, and G. Taccetti. "71 Follow-up of P. aeruginosa eradication in CF patients by RAPD analysis." Journal of Cystic Fibrosis 6 (June 2007): S17. http://dx.doi.org/10.1016/s1569-1993(07)60061-7.

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Li, Lingyan, Hongjiang Yang, Shuxiang Lin, and Shiru Jia. "Classification of 17 newly isolated virulent bacteriophages of Pseudomonas aeruginosa." Canadian Journal of Microbiology 56, no. 11 (2010): 925–33. http://dx.doi.org/10.1139/w10-075.

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Seventeen virulent bacteriophages specific to Pseudomonas aeruginosa strains were isolated by screening various environmental samples. These isolated bacteriophages were grouped based on results obtained from restriction fragment analysis of phage genomes, random amplification of polymorphic DNA (RAPD) typing, morphology observations under transmission electron microscope, and host range analysis. All 17 bacteriophages are double-stranded DNA viruses and can be divided into 5 groups based on DNA restriction profiles. A set of 10-mer primers was used in RAPD typing of phages, and similar conclu

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Heiba, Samy A. A., Ibthal S. El-Demerdash, and Shimaa E. Rashad. "Evaluation of Biological Control of Sorghum Strains Using Bacillus Thuringiensis and Pseudomonas Aeruginosa Under Drought Stress." Journal of Advanced Zoology 44, no. 4 (2023): 8–22. http://dx.doi.org/10.17762/jaz.v44i4.1321.

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Background: Sorghum is an economically significant staple food crop for more than half a billion people in developing nations, especially in arid and semi-arid locations where drought stress is a significant limiting factor. Despite usually being regarded as tolerant, sorghum suffers severely from drought stress, which lowers its productivity and nutritional quality throughout its principal cultivation areas. Objective: Improvements in DNA fingerprinting by ISSRs, SSRs, and RAPD markers have also been employed in sorghum genetic modification (GMOs) to enhance the economic characteristics of th

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Schauer, Bernhard, Regina Wald, Verena Urbantke, Igor Loncaric, and Martina Baumgartner. "Tracing Mastitis Pathogens—Epidemiological Investigations of a Pseudomonas aeruginosa Mastitis Outbreak in an Austrian Dairy Herd." Animals 11, no. 2 (2021): 279. http://dx.doi.org/10.3390/ani11020279.

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The present study describes an outbreak of Pseudomonas (P.) aeruginosa mastitis in a 20-cow dairy herd where throughout genotyping of isolates reusable udder towels were identified as the source of infection. Sampling of cows during three herd surveys and bacteriological culturing showed that P. aeruginosa was isolated from nine cows with a total of 13 infected quarters. Mastitis occurred as mild clinical or subclinical infection. P. aeruginosa was additionally isolated from a teat disinfectant solution, containing N-(3-aminopropyl)-N-dodécylpropane-1,3-diamine 1 as active component, and micro

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Verónica, Jocelyne Flores-Velázquez, and Pérez-y.-Terrón Rocío. "Pseudomonas aeruginosa: Mechanisms of resistance to antibiotics and case analysis." GSC Biological and Pharmaceutical Sciences 14, no. 3 (2021): 179–88. https://doi.org/10.5281/zenodo.4657017.

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<em>Pseudomonas aeruginosa</em> is an opportunistic pathogen, causing great concern due to the rapid increase in its resistance to antibiotics. The objective of the research was to describe the resistance mechanisms that <em>P. aeruginosa</em> possesses, as well as to report its behavior against antibiotics in the years 2003 to 2018 in Mexico. A retrospective and longitudinal documentary research was carried out in different digital resources referring to antibiotic resistance in <em>P. aeruginosa</em>. The results showed that the main resistance mechanisms of <em>P. a

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Verónica Jocelyne Flores-Velázquez and Rocío Pérez-y-Terrón. "Pseudomonas aeruginosa: Mechanisms of resistance to antibiotics and case analysis." GSC Biological and Pharmaceutical Sciences 14, no. 3 (2021): 179–88. http://dx.doi.org/10.30574/gscbps.2021.14.3.0066.

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Pseudomonas aeruginosa is an opportunistic pathogen, causing great concern due to the rapid increase in its resistance to antibiotics. The objective of the research was to describe the resistance mechanisms that P. aeruginosa possesses, as well as to report its behavior against antibiotics in the years 2003 to 2018 in Mexico. A retrospective and longitudinal documentary research was carried out in different digital resources referring to antibiotic resistance in P. aeruginosa. The results showed that the main resistance mechanisms of P. aeruginosa are β-lactamases, ejection pumps, mutations in

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Quintero-Garrido, Karen Guadalupe, Fátima Berenice Ramírez-Montiel, Marilú Chávez-Castillo, et al. "Antibacterial behavior and bacterial resistance analysis of P. aeruginosa in contact with copper nanoparticles." Mexican journal of biotechnology 8, no. 1 (2023): 1–20. http://dx.doi.org/10.29267/mxjb.2023.8.1.1.

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The present study describes the antibacterial behavior and the bacterial resistance analysis of extremophile Pseudomonas aeruginosa in contact with copper nanoparticles (CuNPs). For this purpose, green synthesis of CuNPs was performed by combined ultrasound-assisted and chemical reduction methods, obtaining semispherical CuNPs ranging from ca. 4-9 nm. Antibacterial activity (AA) of biosynthesized CuNPs demonstrates an antibacterial inhibition of 85 % (LD85) at 400 μg/mL and a minimum bactericidal concentration (MBC) of 800 μg/mL after 3 h of contact. Bacterial adaptation in contact with CuNPs

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Wang, Ke, Yi-qiang Chen, May M. Salido, et al. "The rapid in vivo evolution of Pseudomonas aeruginosa in ventilator-associated pneumonia patients leads to attenuated virulence." Open Biology 7, no. 9 (2017): 170029. http://dx.doi.org/10.1098/rsob.170029.

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Pseudomonas aeruginosa is an opportunistic pathogen that causes severe airway infections in humans. These infections are usually difficult to treat and associated with high mortality rates. While colonizing the human airways, P. aeruginosa could accumulate genetic mutations that often lead to its better adaptability to the host environment. Understanding these evolutionary traits may provide important clues for the development of effective therapies to treat P. aeruginosa infections. In this study, 25 P. aeruginosa isolates were longitudinally sampled from the airways of four ventilator-associ

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Xu, Yun, Yanqi Wu, Ling Liang, et al. "Real-Time Recombinase Polymerase Amplification (RPA) Detection of Pseudomonas aeruginosa Using Magnetic Nano-Beads for DNA Extraction." Science of Advanced Materials 13, no. 9 (2021): 1657–65. http://dx.doi.org/10.1166/sam.2021.4067.

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Pseudomonas aeruginosa (P. aeruginosa) is a ubiquitous nonfermentative gram-negative bacillus, residing in nature widely as a conditional pathogen that is commonly isolated from nosocomial infection cases, having a larger genome (5.5–7 Mbp). P. aeruginosa possesses great environmental adaptability and higher mutation rates, which accounts for its ability to resist antibiotics. Furthermore, multi-antibiotics-resistant P. aeruginosa has recently been established to be responsible for increased nosocomial infection incidence. Therefore, to detect, diagnose, and treat this life-threatening infecti

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Harris, Anthony D., Sarah S. Jackson, Gwen Robinson, et al. "Pseudomonas aeruginosa Colonization in the Intensive Care Unit: Prevalence, Risk Factors, and Clinical Outcomes." Infection Control & Hospital Epidemiology 37, no. 5 (2016): 544–48. http://dx.doi.org/10.1017/ice.2015.346.

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OBJECTIVETo determine the prevalence of Pseudomonas aeruginosa colonization on intensive care unit (ICU) admission, risk factors for P. aeruginosa colonization, and the incidence of subsequent clinical culture with P. aeruginosa among those colonized and not colonized.METHODSWe conducted a cohort study of patients admitted to a medical or surgical intensive care unit of a tertiary care hospital. Patients had admission perirectal surveillance cultures performed. Risk factors analyzed included comorbidities at admission, age, sex, antibiotics received during current hospitalization before ICU ad

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Tyumentseva, Marina, Yulia Mikhaylova, Anna Prelovskaya, et al. "CRISPR Element Patterns vs. Pathoadaptability of Clinical Pseudomonas aeruginosa Isolates from a Medical Center in Moscow, Russia." Antibiotics 10, no. 11 (2021): 1301. http://dx.doi.org/10.3390/antibiotics10111301.

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Pseudomonas aeruginosa is a member of the ESKAPE opportunistic pathogen group, which includes six species of the most dangerous microbes. This pathogen is characterized by the rapid acquisition of antimicrobial resistance, thus causing major healthcare concerns. This study presents a comprehensive analysis of clinical P. aeruginosa isolates based on whole-genome sequencing data. The isolate collection studied was characterized by a variety of clonal lineages with a domination of high-risk epidemic clones and different CRISPR/Cas element patterns. This is the first report on the coexistence of

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Vasquez-Rifo, Alejandro, Emiliano P. Ricci, and Victor Ambros. "Pseudomonas aeruginosa cleaves the decoding center of Caenorhabditis elegans ribosomes." PLOS Biology 18, no. 12 (2020): e3000969. http://dx.doi.org/10.1371/journal.pbio.3000969.

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Pathogens such as Pseudomonas aeruginosa advantageously modify animal host physiology, for example, by inhibiting host protein synthesis. Translational inhibition of insects and mammalian hosts by P. aeruginosa utilizes the well-known exotoxin A effector. However, for the infection of Caenorhabditis elegans by P. aeruginosa, the precise pathways and mechanism(s) of translational inhibition are not well understood. We found that upon exposure to P. aeruginosa PA14, C. elegans undergoes a rapid loss of intact ribosomes accompanied by the accumulation of ribosomes cleaved at helix 69 (H69) of the

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Liew, Kok Jun, Xinhua Zhang, Xiaohong Cai, et al. "Transcriptome Study of Cold Plasma Treated Pseudomonas aeruginosa." Chiang Mai Journal of Science 50, no. 2 (2023): 1–19. http://dx.doi.org/10.12982/cmjs.2023.014.

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C old plasma is a disinfection technique widely used in food, agricultural, and medical industries. This work used cold plasma to sterilize Pseudomonas aeruginosa and cell survivability was determined. RNA sequencing was used to determine the bacterial responses at 1 minute (T1), 3 minutes (T3), and 5 minutes (T5) of cold plasma treatments. The results show that longer treatment leads to lower cell survivability. Cold plasma induced rapid cell responses in P. aeruginosa. Gene Ontology enrichment analysis showed that T5 had the most enriched terms compared to T1 and T3. The most affected genes

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Protonotariou, Efthymia, Georgios Meletis, Nikoletta Vlachodimou, et al. "Rapid Reversal of Carbapenemase-Producing Pseudomonas aeruginosa Epidemiology from blaVIM- to blaNDM-harbouring Isolates in a Greek Tertiary Care Hospital." Antibiotics 13, no. 8 (2024): 762. http://dx.doi.org/10.3390/antibiotics13080762.

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Carbapenemase-producing Pseudomonas aeruginosa strains present a specific geographical distribution regarding the type of carbapenemase-encoding genes that they harbor. For more than twenty years, VIM-type enzymes were the only major carbapenemases that were detected among P. aeruginosa isolates in Greece until the emergence of NDM-1-encoding P. aeruginosa in early 2023. In the present study, we present the rapid reversal of the carbapenemase-producing P. aeruginosa epidemiology from blaVIM- to blaNDM-harbouring isolates that occurred in our hospital since then. Between January 2023 and Februa

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Islam, Nazrul, Dilruba Ahmed, Nazmul Ahsan, Chowdhury R. Ahsan, and Mahmuda Yasmin. "Phenotypic-genotypic Features of MDR Pseudomonas Aeruginosa and Acinetobacter Baumannii From Dhaka, Bangladesh." Bioresearch Communications 9, no. 2 (2023): 1276–84. http://dx.doi.org/10.3329/brc.v9i2.67079.

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Pseudomonas aeruginosa and Acinetobacter baumannii are one of the most common causes of MBL mediated morbidity and mortality throughout the world. Day-by-day these isolates are showing increasing resistance trends to different antimicrobial agents. But there are few data in Bangladesh, the study was designed to observe the pattern of antimicrobial resistance, prevalence of MBL, AmpC and finally phylogenetic distributions. A total of 200 isolates were analyzed in this study, comprising of 100 MDR-carbapenem resistant P. aeruginosa and 100 MDR-carbapenem resistant A. baumonnii. Isolates were tes

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García-Rivera, Celia, Carmen Molina-Pardines, José M. Haro-Moreno, Mónica Parra Grande, Juan Carlos Rodríguez, and Mario López-Pérez. "Genomic Analysis of Antimicrobial Resistance in Pseudomonas aeruginosa from a “One Health” Perspective." Microorganisms 12, no. 9 (2024): 1770. http://dx.doi.org/10.3390/microorganisms12091770.

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The “One Health” approach provides a comprehensive framework for understanding antimicrobial resistance. This perspective is of particular importance in the study of Pseudomonas aeruginosa, as it is not only a pathogen that affects humans but also persists in environmental reservoirs. To assess evolutionary selection for niche-specific traits, a genomic comparison of 749 P. aeruginosa strains from three environments (clinical, aquatic, and soil) was performed. The results showed that the environment does indeed exert selective pressure on specific traits. The high percentage of persistent geno

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Sartory, David P., Danièle Pauly, Nathalie Garrec, et al. "Evaluation of an MPN test for the rapid enumeration of Pseudomonas aeruginosa in hospital waters." Journal of Water and Health 13, no. 2 (2014): 427–36. http://dx.doi.org/10.2166/wh.2014.187.

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In this study, the performance of a new most probable number (MPN) test (Pseudalert®/Quanti-Tray®) for the enumeration of Pseudomonas aeruginosa from hospital waters was compared with both international and national membrane filtration-based culture methods for P. aeruginosa: ISO 16266:2006 and UK The Microbiology of Drinking Water – Part 8 (MoDW Part 8), which both use Pseudomonas CN agar. The comparison based on the calculation of mean relative differences between the two methods was conducted according to ISO 17994:2014. Using both routine hospital water samples (80 from six laboratories) a

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Ferjani, Sana, Elaa Maamar, Asma Ferjani, Lamia Kanzari, and Ilhem Boutiba Ben Boubaker. "Evaluation of Three Carbapenemase-Phenotypic Detection Methods and Emergence of Diverse VIM and GES Variants among Pseudomonas aeruginosa Isolates in Tunisia." Antibiotics 11, no. 7 (2022): 858. http://dx.doi.org/10.3390/antibiotics11070858.

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Background: Since 2012, few reports on the molecular epidemiology of Pseudomonas aeruginosa were reported in Tunisia. Objectives: This study aimed to evaluate carbapenem-resistance determinants and molecular epidemiology and to compare the carbapenemase-phenotypic detection methods of multidrug-resistant P. aeruginosa isolates. Methods: During a period of four years (2014 to 2017), all imipenem-ceftazidime-resistant P. aeruginosa isolates were retrospectively selected at the microbial laboratory of Charles Nicolle hospital of Tunis. These isolates were examined by the modified Hodge test, modi

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Lagoumintzis, George, Myrto Christofidou, George Dimitracopoulos та Fotini Paliogianni. "Pseudomonas aeruginosa Slime Glycolipoprotein Is a Potent Stimulant of Tumor Necrosis Factor Alpha Gene Expression and Activation of Transcription Activators Nuclear Factor κB and Activator Protein 1 in Human Monocytes". Infection and Immunity 71, № 8 (2003): 4614–22. http://dx.doi.org/10.1128/iai.71.8.4614-4622.2003.

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ABSTRACT Pseudomonas aeruginosa, an opportunistic pathogen, causes infections associated with a high incidence of morbidity and mortality in immunocompromised hosts. Production of tumor necrosis factor alpha (TNF-α), primarily by cells of monocytic lineage, is a crucial event in the course of these infections. During in vivo infections with P. aeruginosa, both lipopolysaccharide (LPS) and extracellular slime glycolipoprotein (GLP) produced by mucoid and nonmucoid strains are released. In the present study, we sought to explore the relative contributions of these two bacterial products to TNF-α

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Hussain, Mubashir, Xu He, Mingyue Chen, et al. "Optical Spectroscopy Based Microfluidic Platform for Detecting Pathogens Using Immunomagnetic Separation." Journal of Nanoelectronics and Optoelectronics 18, no. 11 (2023): 1393–99. http://dx.doi.org/10.1166/jno.2023.3523.

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Rapid detection of pathogens is crucial for controlling pathogenic diseases and improving the quality of food industry. This paper presents a microfluidic platform integrated with optical detection module to rapidly detect Pseudomonas aeruginosa (P. aeruginosa) and Escherichia coli (E. coli). The detection module comprises a microfluidic chip embedded with fiber optics connected to photosensors and a laser source. Initially, the immunomagnetic separation technique was applied to isolate specific pathogens out of testing sample using magnetic particles coated with antibodies. The separated samp

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Moehario, Lucky Hartati, Enty Tjoa, Hans Putranata, Shikha Joon, Daniel Edbert, and Thomas Robertus. "Performance of TDR-300B and VITEK®2 for the identification of Pseudomonas aeruginosa in comparison with VITEK®-MS." Journal of International Medical Research 49, no. 2 (2021): 030006052198989. http://dx.doi.org/10.1177/0300060521989893.

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Objective Automated systems are needed for the rapid and accurate diagnosis of Pseudomonas-associated nosocomial infections among critically ill patients admitted to the intensive care unit. We assessed the performance of TDR-300B and VITEK®2 for the identification of P. aeruginosa using VITEK®-MS as the gold standard. Methods This analytical study employed a cross-sectional approach. First, 44 clinical isolates of P. aeruginosa were collected and refreshed. Next, a single colony of oxidase-positive, gram-negative rods (30 samples) was inoculated into a TDR-300B NF-64 card and VITEK®2 GN casse

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Milojković, Marko, Željka Nenadović, Slaviša Stanković, et al. "Phenotypic and genetic properties of susceptible and multidrug-resistant Pseudomonas aeruginosa isolates in Southern Serbia." Archives of Industrial Hygiene and Toxicology 71, no. 3 (2020): 231–50. http://dx.doi.org/10.2478/aiht-2020-71-3418.

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AbstractDrug resistance of Pseudomonas aeruginosa is a leading problem in hospital infections. The aim of this study was to determine the best molecular genetic discrimination method for Pseudomonas spp. isolates among 94 outpatients and inpatients and see their grouping by phenotype characteristics (biofilm formation, frequency of serotypes, pigmentation, production of different class of beta-lactamases, and susceptibility to different antibiotic classes) and genotype. The most common serotypes were P1, P6, and P11, while co-productions of pyoverdine and pyocyanin were observed in 70 % of iso

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Sørensen, Jan, Jan Skouv, Anita Jørgensen, and Ole Nybroe. "Rapid identification of environmental isolates of Pseudomonas aeruginosa, P. fluorescens and P. putida by SDS-PAGE analysis of whole-cell protein patterns." FEMS Microbiology Ecology 10, no. 1 (1992): 41–50. http://dx.doi.org/10.1111/j.1574-6941.1992.tb01647.x.

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Sørensen, J. "Rapid identification of environmental isolates of Pseudomonas aeruginosa, P. fluorescens and P. putida by SDS-PAGE analysis of whole-cell protein patterns." FEMS Microbiology Letters 101, no. 1 (1992): 41–50. http://dx.doi.org/10.1016/0378-1097(92)90696-l.

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Sørensen, J. "Rapid identification of environmental isolates of Pseudomonas aeruginosa, P. fluorescens and P. putida by SDS-PAGE analysis of whole-cell protein patterns." FEMS Microbiology Ecology 101, no. 1 (1992): 41–50. http://dx.doi.org/10.1016/0168-6496(92)90070-a.

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SÃ¸rensen, Jan, Jan Skouv, Anita JÃ¸rgensen, and Ole Nybroe. "Rapid identification of environmental isolates ofPseudomonas aeruginosa, P. fluorescensandP. putidaby SDS-PAGE analysis of whole-cell protein patterns." FEMS Microbiology Letters 101, no. 1 (1992): 41–50. http://dx.doi.org/10.1111/j.1574-6968.1992.tb05760.x.

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Gopalraaj, Jhanani, and Krishnakumar Velayudhannair. "Antimicrobial potential of selected fruit peel extracts against multidrug-resistant bacteria: An eco-friendly approach." Journal of Applied and Natural Science 17, no. 1 (2025): 152–61. https://doi.org/10.31018/jans.v17i1.6197.

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The rapid rise of multidrug resistance (MDR) bacteria due to the misuse of antibiotics necessitates the discovery of alternative therapeutic agents. This study investigates the antimicrobial properties of methanolic extract of selected fruit peels: Carica papaya, Ananas comosus, Musa acuminata, and Punica granatum. These extracts were tested against gram-positive bacteria (Staphylococcus aureus) and gram-negative bacteria (Pseudomonas aeruginosa and Klebsiella pneumoniae). The findings indicated that all four fruit peel extracts exhibited antimicrobial activity against all the selected pathoge

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Stingley, Robin L., Wen Zou, Thomas M. Heinze, Huizhong Chen, and Carl E. Cerniglia. "Metabolism of azo dyes by human skin microbiota." Journal of Medical Microbiology 59, no. 1 (2010): 108–14. http://dx.doi.org/10.1099/jmm.0.012617-0.

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Reduction of Methyl Red (MR) and Orange II (Or II) by 26 human skin bacterial species was monitored by a rapid spectrophotometric assay. The analysis indicated that skin bacteria, representing the genera Staphylococcus, Corynebacterium, Micrococcus, Dermacoccus and Kocuria, were able to reduce MR by 74–100 % in 24 h, with only three species unable to reduce completely the dye in that time. Among the species tested, only Corynebacterium xerosis was unable to reduce Or II to any degree by 24 h, and only Staphylococcus delphini, Staphylococcus sciuri subsp. sciuri and Pseudomonas aeruginosa were

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ANTON, Maria. "The genetic basis of gram-negative bacteria resistant to antimicrobials isolated from invasive infections in the Republic of Moldova." One Health & Risk Management 5, no. 2 (2024): 34–41. http://dx.doi.org/10.38045/ohrm.2024.2.04.

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Introduction. Despite the efforts made and measures taken to combat antimicrobial resistance, alarming levels of resistance in gram-negative bacteria continue to be reported on a global scale. The antimicrobial resistance mechanisms of these bacteria represent the main cause of therapeutic failures. Material and methods. A retrospective analysis of strains of E. coli, K. pneumoniae, P. aeruginosa, and A. baumannii isolated from patients with invasive infections was conducted for the period 2020-2023. Screening for carbapenemase-producing strains was performed based on sensitivity to antimicrob

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Huq, Md Amdadul, and Shahina Akter. "Bacterial Mediated Rapid and Facile Synthesis of Silver Nanoparticles and Their Antimicrobial Efficacy against Pathogenic Microorganisms." Materials 14, no. 10 (2021): 2615. http://dx.doi.org/10.3390/ma14102615.

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In the present study, silver nanoparticles (AgNPs), biosynthesized using culture supernatant of bacterial strain Paenarthrobacter nicotinovorans MAHUQ-43, were characterized and their antimicrobial activity was investigated against both Gram-positive Bacillus cereus and Gram-negative bacteria Pseudomonas aeruginosa. Bacterial-mediated synthesized AgNPs were characterized by UV-Visible (UV-Vis) spectrophotometer, field emission-transmission electron microscopy (FE-TEM), energy dispersive X-ray (EDX), X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, and dynamic light scat

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Kang, Dingyu, Hai Lin, Qiang Li, et al. "Enhanced Oil Recovery in a Co-Culture System of Pseudomonas aeruginosa and Bacillus subtilis." Microorganisms 12, no. 11 (2024): 2343. http://dx.doi.org/10.3390/microorganisms12112343.

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Microbial enhanced oil recovery (MEOR) is a promising technology for oil field extraction. This study investigated a co-culture system of Pseudomonas aeruginosa and Bacillus subtilis to increase MEOR efficacy. We analyzed bacterial growth, biosurfactant production, and crude oil emulsified performance under different inoculation ratios. Compared to single cultures, the co-culture system showed superior growth and functional expression, with an optimal inoculation ratio of 1:1. Quantitative assessments of the cell numbers and biosurfactant production during the co-culture revealed that rapid B.

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Hernando-Amado, Sara, Fernando Sanz-García, and José Luis Martínez. "Rapid and robust evolution of collateral sensitivity in Pseudomonas aeruginosa antibiotic-resistant mutants." Science Advances 6, no. 32 (2020): eaba5493. http://dx.doi.org/10.1126/sciadv.aba5493.

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The analysis of trade-offs, as collateral sensitivity, associated with the acquisition of antibiotic resistance, is mainly based on the use of model strains. However, the possibility of exploiting these trade-offs for fighting already resistant isolates has not been addressed in depth, despite the fact that bacterial pathogens are frequently antibiotic-resistant, forming either homogeneous or heterogeneous populations. Using a set of Pseudomonas aeruginosa-resistant mutants, we found that ceftazidime selects pyomelanogenic tobramycin-hypersusceptible mutants presenting chromosomal deletions in

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Göpfert, Lisa, Julia Klüpfel, Charlotte Heinritz, Martin Elsner, and Michael Seidel. "Macroporous epoxy-based monoliths for rapid quantification of Pseudomonas aeruginosa by adsorption elution method optimized for qPCR." Analytical and Bioanalytical Chemistry 412, no. 29 (2020): 8185–95. http://dx.doi.org/10.1007/s00216-020-02956-3.

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Abstract Pseudomonas aeruginosa contaminations in tap water systems have caused severe health problems in both hospital and household settings. To ensure fast and reliable detection, culture-independent methods are recommendable. However, the typically low cell number in water samples requires sample enrichment prior to analysis. Therefore, we developed and optimized an adsorption elution method using monolithic adsorption filtration and subsequent centrifugal ultrafiltration that can be combined with culture-independent detection methods. The principle of adsorption of Pseudomonas aeruginosa

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Kang, Min-Gyun, Fazlurrahman Khan, Du-Min Jo, DoKyung Oh, Nazia Tabassum, and Young-Mog Kim. "Antibiofilm and Antivirulence Activities of Gold and Zinc Oxide Nanoparticles Synthesized from Kimchi-Isolated Leuconostoc sp. Strain C2." Antibiotics 11, no. 11 (2022): 1524. http://dx.doi.org/10.3390/antibiotics11111524.

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The rapid emergence of antimicrobial resistance (AMR) among bacterial pathogens results in antimicrobial treatment failure and the high mortality rate associated with AMR. The application of nanoparticles synthesized from probiotics will be widely accepted due to their efficacy and biocompatibility in treating microbial infections in humans. The current work sought to isolate and identify lactic acid bacteria (LAB) from Kimchi. Based on 16S rRNA gene sequencing, the LAB isolate C2 was identified as a member of the genus Leuconostoc. The obtained supernatant from Leuconostoc sp. strain C2 was e

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Huq, Md Amdadul. "Green Synthesis of Silver Nanoparticles Using Pseudoduganella eburnea MAHUQ-39 and Their Antimicrobial Mechanisms Investigation against Drug Resistant Human Pathogens." International Journal of Molecular Sciences 21, no. 4 (2020): 1510. http://dx.doi.org/10.3390/ijms21041510.

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Silver nanoparticles (AgNPs) have shown great promise in biomedical applications. The exact mechanism and mode of action of AgNPs regarding antimicrobial activity are still not well known. Moreover, synthesis of nanoparticles by physical and chemical methods is expensive and not ecofriendly. This study highlights the green, rapid, facile, cost-effective and ecofriendly synthesis of AgNPs using Pseudoduganella eburnea MAHUQ-39 and also investigates their antibacterial mechanisms. The transmission electron microscopy (TEM) image revealed a spherical shape of the AgNPs. The size of the synthesize

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Zechman, James M., and John N. Labows Jr. "Volatiles of Pseudomonas aeruginosa and related species by automated headspace concentration – gas chromatography." Canadian Journal of Microbiology 31, no. 3 (1985): 232–37. http://dx.doi.org/10.1139/m85-045.

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The volatile metabolites of three strains of Pseudomonas aeruginosa and one strain each of Pseudomonas cepacia, Pseudomonas maltophilia, Pseudomonas fluorescens, and Pseudomonas putida were analyzed using an automated headspace concentrator incorporating a gas chromatograph. The procedure does not require sample preparation and automates the entire analytical sequence to yield reproducible profiles of volatile constituents. Gas chromatographic profiles of the volatile metabolites of each species were obtained using a 20-min concentration period and two fused silica capillary columns of differe

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Jongers, Bart’s, An Hotterbeekx, Kenny Bielen, et al. "Identification of Potential Urinary Metabolite Biomarkers of Pseudomonas aeruginosa Ventilator-Associated Pneumonia." Biomarker Insights 17 (January 2022): 117727192210991. http://dx.doi.org/10.1177/11772719221099131.

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Introduction: Ventilator-associated pneumonia (VAP) caused by Pseudomonas aeruginosa is a major cause of morbidity and mortality in hospital intensive care units (ICU). Rapid identification of P. aeruginosa-derived markers in easily accessible patients’ samples can enable an early detection of P. aeruginosa VAP (VAP-PA), thereby stewarding antibiotic use and improving clinical outcomes. Methods: Metabolites were analysed using liquid chromatography-mass spectrometry (LC-MS) in prospectively collected urine samples from mechanically ventilated patients admitted to the Antwerp University Hospita

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Hirakata, Yoichi, Koichi Izumikawa, Toshiyuki Yamaguchi та ін. "Rapid Detection and Evaluation of Clinical Characteristics of Emerging Multiple-Drug-Resistant Gram-Negative Rods Carrying the Metallo-β-Lactamase GeneblaIMP". Antimicrobial Agents and Chemotherapy 42, № 8 (1998): 2006–11. http://dx.doi.org/10.1128/aac.42.8.2006.

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Gram-negative rods (GNR) carrying the transferable carbapenem resistance gene blaIMP, includingPseudomonas aeruginosa and Serratia marcescens, have been isolated from more than 20 hospitals in Japan. Although the emergence of such multiple-drug-resistant bacteria is of utmost clinical concern, little information in regard to the distribution ofblaIMP-positive GNR in hospitals and the clinical characteristics of infected patients is available. To address this, a system for the rapid detection of theblaIMP gene with a simple DNA preparation and by enzymatic detection of PCR products was develope

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Abdul Rahim, Muhammad Khairulanwar, Nur Mas Ayu Jamaludin, Jacinta Santhanam, Azrul Azlan Hamzah, and Muhamad Ramdzan Buyong. "Rapid ESKAPE Pathogens Detection Method using Tapered Dielectrophoresis Electrodes via Crossover Frequency Analysis." Sains Malaysiana 49, no. 12 (2020): 2913–25. http://dx.doi.org/10.17576/jsm-2020-4912-04.

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This paper introduces the versatile of an electrokinetic technique by using the non-uniform electric field for dielectrophoresis (DEP) application. This technique is defined as electromicrofluidics. The potential application for portable and real time detection method of Enterococcus faecium (EF), Staphylococcus aureus (SA), Klebsiella pneumoniae (KP), Acinetobacter baumannii (AB), Pseudomonas aeruginosa (PA) and Enterobacter aerogenes (EA), which are the (ESKAPE) bacteria. The MATLAB analytical modelling was used in simulating the polarisation factor and velocities of bacteria based on Clausi

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Ghozzi, Rafiaa, Philippe Morand, Agnes Ferroni, et al. "Capillary Electrophoresis–Single-Strand Conformation Polymorphism Analysis for Rapid Identification of Pseudomonas aeruginosa and Other Gram-Negative Nonfermenting Bacilli Recovered from Patients with Cystic Fibrosis." Journal of Clinical Microbiology 37, no. 10 (1999): 3374–79. http://dx.doi.org/10.1128/jcm.37.10.3374-3379.1999.

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We used capillary electrophoresis–single-strand conformation polymorphism (CE-SSCP) analysis of PCR-amplified 16S rRNA gene fragments for rapid identification of Pseudomonas aeruginosa and other gram-negative nonfermenting bacilli isolated from patients with cystic fibrosis (CF). Target sequences were amplified by using forward and reverse primers labeled with various fluorescent dyes. The labeled PCR products were denatured by heating and separated by capillary gel electrophoresis with an automated DNA sequencer. Data were analyzed with GeneScan 672 software. This program made it possible to

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Wilkinson, Lauren V., Morgan A. Alford, Shannon R. Coleman, et al. "Peptide 1018 inhibits swarming and influences Anr-regulated gene expression downstream of the stringent stress response in Pseudomonas aeruginosa." PLOS ONE 16, no. 4 (2021): e0250977. http://dx.doi.org/10.1371/journal.pone.0250977.

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Pseudomonas aeruginosa is a ubiquitous opportunistic pathogen that causes considerable human morbidity and mortality, particularly in nosocomial infections and individuals with cystic fibrosis. P. aeruginosa can adapt to surface growth by undergoing swarming motility, a rapid multicellular movement that occurs on viscous soft surfaces with amino acids as a nitrogen source. Here we tested the small synthetic host defense peptide, innate defense regulator 1018, and found that it inhibited swarming motility at concentrations as low as 0.75 μg/ml, well below the MIC for strain PA14 planktonic cell

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Candela, Ana, Manuel J. Arroyo, María Sánchez-Cueto, et al. "Rapid Discrimination of Pseudomonas aeruginosa ST175 Isolates Involved in a Nosocomial Outbreak Using MALDI-TOF Mass Spectrometry and FTIR Spectroscopy Coupled with Machine Learning." Transboundary and Emerging Diseases 2023 (September 7, 2023): 1–11. http://dx.doi.org/10.1155/2023/8649429.

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The goal of this study was to evaluate matrix-assisted laser desorption ionization–iime of flight mass spectrometry (MALDI-TOF MS) and Fourier-transform infrared spectroscopy (FTIR-S) as diagnostic alternatives to DNA-based methods for the detection of Pseudomonas aeruginosa sequence type (ST) 175 isolates involved in a hospital outbreak. For this purpose, 27 P. aeruginosa isolates from an outbreak detected in the Hematology department of our hospital were analyzed by the above-mentioned methodologies. Previously, these isolates had been characterized by pulse-field gel electrophoresis (PFGE)

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Williams, Calistus C., G. A. Ajibade, Victoria Moltong Yilwa, and Nwankwo Cornelius Tochukwu. "Signaling Molecules in Pseudomonas aeruginosa Response to Antibiotics at Sub-Inhibitory Concentrations." Asian Journal of Biotechnology and Bioresource Technology 10, no. 4 (2024): 60–71. http://dx.doi.org/10.9734/ajb2t/2024/v10i4219.

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Signaling Molecules are N-acylhomoserine lactones (AHLs) that form the Pseudomonas aeruginosa cell information system which determines gene expressions in a population dependent manner called quorum sensing (QS). Signal molecules, which are chemically varied, control genes expressions to antibiotics resistance, pathogenicity, motility, biofilm development, bioluminescence, secondary metabolite production, and plasmid transfer. This research was aimed to identify the signaling molecules in Pseudomonas aeruginosa response to antibiotics at sub-inhibitory concentrations. One hundred and fifty (15

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ABOU-DOBARA, M. I., M. A. DEYAB, E. M. ELSAWY, and H. H. MOHAMED. "Antibiotic Susceptibility and Genotype Patterns of Escherichia coli, Klebsiella pneumonia and Pseudomonas aeruginosa Isolated from Urinary Tract Infected Patients." Polish Journal of Microbiology 59, no. 3 (2010): 207–12. http://dx.doi.org/10.33073/pjm-2010-032.

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Thirty nine isolates of Escherichia coli, twenty two isolates of Klebsiella pneumoniae and sixteen isolates of Pseudomonas aeruginosa isolated from urinary tract infected patients were analyzed by antimicrobial susceptibility typing and random amplified polymorphic DNA (RAPD)-PCR. Antibiotic susceptibility testing was carried out by microdilution and E Test methods. From the antibiotic susceptibility, ten patterns were recorded (four for E. coli, three for K. pneumoniae and three for P. aeruginosa respectively). Furthermore, genotyping showed seventeen RAPD patterns (seven for E. coli, five fo

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Martins-Oliveira, Inês, Blanca Pérez-Viso, Rosário Gomes, et al. "Phenotypic Ultra-Rapid Antimicrobial Susceptibility Testing for Ceftazidime–Avibactam: In Support of Antimicrobial Stewardship." Microorganisms 13, no. 2 (2025): 414. https://doi.org/10.3390/microorganisms13020414.

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Ceftazidime–avibactam (CZA) is a potent broad-spectrum drug combination covering extended-spectrum β-lactamases, AmpC, and carbapenemases of class A and D, OXA-48-type producers. Rapid antimicrobial susceptibility testing is crucial for the timely de-escalation/escalation of therapy. We evaluate CZA susceptibility using the CE-IVD FASTgramneg kit (FASTinov®), a ground-breaking 2 h assay, based on flow cytometry technology for antimicrobial susceptibility testing. The assay involved rapid bacterial extraction and purification from positive blood cultures (PBCs), followed by a 1 h 37 °C incubati

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Gooderham, W. James, Manjeet Bains, Joseph B. McPhee, Irith Wiegand, and Robert E. W. Hancock. "Induction by Cationic Antimicrobial Peptides and Involvement in Intrinsic Polymyxin and Antimicrobial Peptide Resistance, Biofilm Formation, and Swarming Motility of PsrA in Pseudomonas aeruginosa." Journal of Bacteriology 190, no. 16 (2008): 5624–34. http://dx.doi.org/10.1128/jb.00594-08.

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ABSTRACT Pseudomonas aeruginosa is an important opportunistic pathogen that causes infections that can be extremely difficult to treat due to its high intrinsic antibiotic resistance and broad repertoire of virulence factors, both of which are highly regulated. It is demonstrated here that the psrA gene, encoding a transcriptional regulator, was upregulated in response to subinhibitory concentrations of cationic antimicrobial peptides. Compared to the wild type and the complemented mutant, a P. aeruginosa PAO1 psrA::Tn5 mutant displayed intrinsic supersusceptibility to polymyxin B, a last-reso

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Capatina, Denisa, Teodora Lupoi, Bogdan Feier, Diana Olah, Cecilia Cristea, and Radu Oprean. "Highly Sensitive Detection of PQS Quorum Sensing in Pseudomonas Aeruginosa Using Screen-Printed Electrodes Modified with Nanomaterials." Biosensors 12, no. 8 (2022): 638. http://dx.doi.org/10.3390/bios12080638.

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The rapid diagnosis of Pseudomonas aeruginosa infection is very important because this bacterium is one of the main sources of healthcare-associated infections. Pseudomonas quinolone signal (PQS) is a specific molecule for quorum sensing (QS) in P. aeruginosa, a form of cell-to-cell bacterial communication and its levels can allow the determination of the bacterial population. In this study, the development of the first electrochemical detection of PQS using screen-printed electrodes modified with carbon nanotubes (CNT-SPE) is reported. The electrochemical fingerprint of PQS was determined usi

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