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Journal articles on the topic 'RAPD analysis'

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Published: 4 June 2021

Last updated: 10 February 2022

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1

Raddová, J., M. Beránek, I. Oukropec, and M. Vachůn. "RAPD Analysis of peaches within Czech National collection." Czech Journal of Genetics and Plant Breeding 39, No. 4 (November 23, 2011): 113–19. http://dx.doi.org/10.17221/3728-cjgpb.

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The Random Amplified Polymorphic DNA (RAPD) technique was used to study the genetic diversity and relationships within the collection of the Czech National Plant Genetic Resources (PGR) of peaches (Prunus persica L.). The aim of the work was to elaborate a dendrogram of genetic similarity and to divide collection into clusters. 46 primers were applied to 6 cultivars differing in the place of origin, the fruit shape, the fruit colour, and in some other morphological characteristics. 12 primers were chosen which gave polymorphic repeatable strong and middle strong bands. They were subsequently used for the RAPD reactions within the whole collection of peaches. The selected RAPD primers distinguished 28 peach cultivars and RAPD data were used to group the accessions analysed. Almonds and peach × almond hybrids were clearly separated in the frame of the whole collection. The grouping corresponded to the botanical system, to the available information about pedigree, and to the cultivars description.  

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2

Gawel, Nick J., Rory Mellinger, Eric Stout, and R. Sauve. "RAPD Analysis of Acer." HortScience 30, no. 4 (July 1995): 813F—813. http://dx.doi.org/10.21273/hortsci.30.4.813f.

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DNA from 27 Acer species was used for RAPD analysis. A relatively high number of phylogenically informative polymorphisms were detected, as would be expected in intraspecific comparisons. Principle coordinates analysis was used to discern groupings among the species and a RAPD-based phylogeny was constructed. As expected when making comparisons among species, very high levels of polymorphism were found. Cultivars that grouped together in the principle components analysis also grouped together in the phylogenic analysis. Parts of the phylogenic analysis do not agree with morphology-based phylogenies. This may be due to poor correlation between morphological and DNA markers, or perhaps RAPDs may be too discriminatory to be used for interspecies comparisons. The extremely high level of between-species variation coupled with the low level of within-species variation, indicates the potential of DNA-based identification and discrimination of Acer species is high.

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3

Vlastníková, H., K. Moravcová, and M. Pidra. "The RAPD analysis of several cultivars of grapevine (Vitis viniferaL.) and their clones." Horticultural Science 31, No. 4 (November 25, 2011): 136–39. http://dx.doi.org/10.17221/3807-hortsci.

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Nine identification RAPD markers (Moravcová et al. 2003) were used to distinguish 24 clones and grapevine cultivars. No polymorphism was detected among all the tested clones of Chardonnay, Pinot gris and Zweigeltrebe from Pole&scaron;ovice. Pinot noir, Pinot gris, Pinot blanc and Pinot Meunier were indistinguishable within clones, they also showed the identical RAPD profile within cultivars (except discussed sample No. 26). On the other hand, Auxerrois as a relative to cultivars of Pinot group showed unique patterns and may be classified as a different cultivar. Some irregularities within the cultivars of Pinot family from Oblekovice were also found, several of them gave different results from those expected: Pinot blanc sample 26 has the RAPD profile typical of Chardonnay. A new abnormal RAPD pattern as a marker of typical Chardonnay and Pinot profiles was observed in two cases. While RAPD banding patterns could not distinguish between the known clones, they were useful for distinguishing between phenotypically similar cultivars and for assessing the origins of cultivars thought to have originated as sports.    

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4

Ozbey, G., Ertas HB, and A. Muz. "Random amplified polymorphic DNA (RAPD) analysis of Ornithobacterium rhinotracheale strains isolated from chickens in Turkey." Veterinární Medicína 50, No. 12 (March 28, 2012): 526–30. http://dx.doi.org/10.17221/5660-vetmed.

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Six field strains of Ornithobacterium rhinotracheale isolated from chickens in Elazig province located in the East of Turkey were typed by serotyping and random amplified polymorphic DNA assay using a random primer (OPG-11). Using the AGP test used for serotyping, serotype A was found to be the predominant serotype, only one strain was serotyped as serotype B. By RAPD assay, the tested ORT strains were found to have different RAPD profiles. In addition, the RAPD assay showed almost similar DNA profiles among the tested strains of the serotypes A, B, D and E. The strain of serotype C did give a different RAPD profile. Within strains of the same serotype (A), different profiles were found but the strain of serotype (B) had an identical profile as strains of serotype A. This study suggests that more genotypes of ORT strains are present within the same serotype and thus that no relationship exists between the RAPD pattern of ORT and their serotype.

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5

Tigano, Myrian S., and Salah Aljanabi. "RAPD Analysis of Nomuraea rileyi." Journal of Invertebrate Pathology 75, no. 3 (April 2000): 240–42. http://dx.doi.org/10.1006/jipa.1999.4920.

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6

Säull, T., C. Lind-Halldén, and C. Halldén. "Primer Mixtures in RAPD Analysis." Hereditas 132, no. 3 (May 5, 2004): 203–8. http://dx.doi.org/10.1111/j.1601-5223.2000.00203.x.

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7

Rasmussen, H. N., J. E. Olsen, and O. F. Rasmussen. "RAPD analysis of Yersinia enterocolitica." Letters in Applied Microbiology 19, no. 5 (November 1994): 359–62. http://dx.doi.org/10.1111/j.1472-765x.1994.tb00475.x.

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8

Miyata, M., T. Aoki, V. Inglis, T. Yoshida, and M. Endo. "RAPD analysis ofAeromonas salmonicidaandAeromonas hydrophila." Journal of Applied Bacteriology 79, no. 2 (August 1995): 181–85. http://dx.doi.org/10.1111/j.1365-2672.1995.tb00933.x.

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9

Scott, Michelle Pellissier, Kenneth M. Haymes, and Scott M. Williams. "Parentage analysis using RAPD PCR." Nucleic Acids Research 20, no. 20 (1992): 5493. http://dx.doi.org/10.1093/nar/20.20.5493.

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10

Baleiras Couto, M. M., J. M. B. M. van der Vossen, H. Hofstra, and J. H. J. Huis in 't Veld. "RAPD analysis: a rapid technique for differentiation of spoilage yeasts." International Journal of Food Microbiology 24, no. 1-2 (December 1994): 249–60. http://dx.doi.org/10.1016/0168-1605(94)90123-6.

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11

Warghat, Nandkishor, Navin Sharma, Punam Thakur, and Mumtaz Baig. "Genetic Diversity Analysis of Crab Spider (Araneae: Thomisidae) based on RAPD-PCR." Indian Journal of Applied Research 4, no. 8 (October 1, 2011): 1–5. http://dx.doi.org/10.15373/2249555x/august2014/191.

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12

Ling, Jing-Tian, Nick Gawel, and Roger J. Sauve. "RAPD Analysis of Hosta Species and Cultivars." HortScience 30, no. 4 (July 1995): 811E—811. http://dx.doi.org/10.21273/hortsci.30.4.811e.

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The genus of Hosta (plantain lily) is a shade-loving herbaceous plant with attractive foliage. Confusion exists in the genus regarding nomenclature and taxonomy. In this study, the possibility of application of RAPD markers to characterize Hosta species and cultivars was investigated. DNA was extracted from 28 Hosta species and cultivars. Thirty-six of 37 primers generated RAPD markers. Phylogenic analysis and principal components analysis showed groupings among cultivars. Results indicated that H. plantaginea and H. ventricosa were the most distant from the other tested species and cultivars. These results suggest RAPDs may be useful in the identification and analysis of relationships among Hosta.

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13

Jan, C. H., D. H. Byrne, J. Manhart, and H. Wilson. "Rose Germplasm Analysis with RAPD Markers." HortScience 34, no. 2 (April 1999): 341–45. http://dx.doi.org/10.21273/hortsci.34.2.341.

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The genus Rosa consists of more than 100 species classified into four subgenera, Eurosa, Platyrhodon, Hesperhodos, and Hulthemia, and distributed widely throughout the northern hemisphere. The subgenus Eurosa includes 11 sections. The other subgenera are monotypic. One hundred and nineteen accessions and 213 markers of 36 rose species that include eight sections of the subgenus Eurosa and one species each from the subgenera Hesperhodos and Platyrhodon were used to calculate a similarity matrix, which was clustered with the unweighted pair group method using arithmetic means (UPGMA). The RAPD markers distinguished between all the rose accessions, and species grouped into their respective sections. Therefore, classification of Rosa using RAPD data generally supports traditional classification. The Asian rose sections (Laevigatae, Banksianae, Bracteatae, Pimpinellifoliae, Chinenses, and Synstylae) were consistently separated from the primarily North American sections (Cassiorhodon and Carolinae). The Cassiorhodon and Carolinae sections were grouped together with the subgenera Hesperhodos and Platyrhodon. Both subgenera separated out at the same level as sections within the subgenus Eurosa, suggesting that they are more appropriately classified as sections within the subgenus Eurosa. Sections Cassiorhodon and Carolinae overlapped, and are probably best grouped as one section as previously suggested.

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14

Mandal, P. K., I. M. Santha, and S. L. Mehta. "RAPD Analysis of Lathyrus sativus Somaclones." Journal of Plant Biochemistry and Biotechnology 5, no. 2 (July 1996): 83–86. http://dx.doi.org/10.1007/bf03262987.

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15

Okatani, Alexandre Tomomitsu, Hideki Hayashidani, Toshio Takahashi, Takahide Taniguchi, Masuo Ogawa, and Ken-ichi Kaneko. "Randomly Amplified Polymorphic DNA Analysis ofErysipelothrix spp." Journal of Clinical Microbiology 38, no. 12 (2000): 4332–36. http://dx.doi.org/10.1128/jcm.38.12.4332-4336.2000.

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The usefulness of randomly amplified polymorphic DNA method (RAPD) to identify each species of genus Erysipelothrix and for epidemiological analysis of this genus was studied. Eighty-one strains and 18 random primers were tested. Among the tested primers, the primers NK51 (GGTGGTGGTATC) and NK6 (CCCGCGCCCC) produced noticeable results. The primer NK51 revealed four species-specific RAPD patterns. Of the 66 strains of E. rhusiopathiae, 64 had the same unique band of 884 bp. Of the 12 strains of E. tonsillarum, 11 produced a 1,265-bp band. In addition, two strains, previously thought to be E. rhusiopathiae, produced the 1,265-bp band, suggesting that they had been misclassified. One strain of E. tonsillarumproduced the 884-bp band, suggesting that it too was E. rhusiopathiae. The E. rhusiopathiae strain of serovar 13 produced a 650-bp band, and the strain of serovar 18 produced a clear 420-bp band as well as three weak bands of 1,265, 918, and 444 bp. The primer NK6 revealed 14 RAPD patterns that were not serovar specific. However, different patterns were produced among strains of the same serovar showing that the RAPD method is able to identify the genetic variations of strains of this genus and can rapidly and easily differentiate strains of the same serovar. Based on these results, we concluded that the RAPD method with primers NK51 and NK6 is a rapid and reliable method to identify the species of this genus; we also concluded that this method might be a useful tool for the epidemiological analysis of the Erysipelothrix species.

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16

Noli, Enrico, Silvio Salvi, and Roberto Tuberosa. "Comparative analysis of genetic relationships in barley based on RFLP and RAPD markers." Genome 40, no. 5 (October 1, 1997): 607–16. http://dx.doi.org/10.1139/g97-080.

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Genetic relationships have seldom been analyzed with different types of molecular markers in order to compare the information provided by each marker class. We investigated genetic relationships among nine barley cultivars using separate cluster analyses based on restriction fragment length polymorphisms (RFLPs) and random amplified polymorphic DNAs (RAPDs). Genomic DNA restricted with three enzymes and hybridized with 68 probes revealed 415 RFLPs (74.2% of all bands). Among the 128 primers used for RAPD analysis, 100 provided a reproducible profile, 89 of which revealed 202 polymorphic and 561 monomorphic bands (26.5 and 73.5%, respectively). A nonrandom distribution of 62 RAPDs with a tendency to cluster near centromeric regions was produced when these RAPDs were mapped using 76 doubled-haploid lines derived from a cross between two of the nine cultivars. The correlation between the RFLP and RAPD similarity matrices computed for the 36 pairwise comparisons among the nine cultivars was equal to 0.83. The dendrograms obtained by cluster analyses of the RFLP and RAPD data differed. These results indicate that in barley the information provided by RFLPs and RAPDs is not equivalent, most likely as a consequence of the fact that the two marker classes explore, at least in part, different portions of the genome.Key words: Hordeum vulgare L., genetic distance, molecular markers, cluster analysis.

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17

Wu, Y. J., Y. Chen, J. Wang, C. X. Zhu, and B. L. Xu. "RAPD analysis of jasmine rice-specific genomic structure." Genome 49, no. 6 (June 1, 2006): 716–19. http://dx.doi.org/10.1139/g06-018.

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Total genomic DNA was extracted from 29 samples of rice seed, including jasmine rice Oryza sativa L. subsp indica 'KDML105', 'KDML105'-derived varieties, nonaromatic Thailand rice, and japonica rice. Polymorphism in RAPD profiles was analyzed to explore the genomic structure specific to jasmine rice. The degree of band sharing was used to evaluate genetic distance between varieties and to construct a phylogenetic tree. RD15, CNTLR85033, and CNT87040 were found to be closest to 'KDML105', which was consistent with the true relation among them. Four RAPD fragments that cooperatively distinguished jasmine rice from others were cloned and sequenced. PCR amplification using pairs of primers designed specifically further confirmed the credibility of the RAPD result. Comparison through Genbank revealed that a 454 bp RAPD band was similar to the first intron of a putative Cf2/Cf5 disease resistance gene and a 1107 bp RAPD band similar to a wall-associated kinase (wak) gene sequence.Key words: Jasmine rice, RAPD, specific sequence.

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18

Bayazit, S., B. Imrak, A. Küden, and M. Kemal Güngör. "RAPD analysis of genetic relatedness among selected quince (Cydonia oblonga Mill.) accessions from different parts of Turkey." Horticultural Science 38, No. 4 (November 15, 2011): 134–41. http://dx.doi.org/10.17221/97/2011-hortsci.

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Quince (Cydonia oblonga Mill.) is a minor fruit crop, which is primarily used for marmalade, jam, sauce and as rootstocks for pears. Different cultivated and local quince genotypes are grown in almost all parts of Turkey for fruit usage. In this study, randomly amplified polymorphic DNA (RAPD) technology was used to study the genetic relationships among 13 quince accessions selected from different parts of Turkey. Thirty decamer primers were used and 14 of them did not produce any polymorphism. The remaining 16 primers ranged in their amplification fragments between one (P-402, P-437, OPA 10, OPA 16, OPA 18 and OPA-19) and five (OPA-06 and OPA-07). The size of fragments varied from 100 to 1500 bp. Similarity values among the studied genotypes ranged between 0.483 and 0.925. The resulting dendrogram clustered into two groups (0.69 similarity value) based on evaluation of genetic similarities and differences. The results suggest that RAPD analysis could be used to distinguish and determine genetic variation among quince accessions. Also, the obtained clustering based on RAPD markers agreed to some extent with the geographical origin of the studied set of quince accessions.

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19

Lee, In-Ho, Jong-In Park, Oon-Sup Jeong, Hyo-Jin Jung, Gun-Ho Jung, and Ill-Sup Nou. "Genetic Relationship Based on RAPD Analysis of Yeosu Dolsan Leaf Mustard(Brassica juncea)." Journal of Life Science 20, no. 1 (January 30, 2010): 66–70. http://dx.doi.org/10.5352/jls.2010.20.1.066.

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20

Ii, Y., A. Uragami, Y. Uno, M. Kanechi, and N. Inagaki. "RAPD-based analysis of differences between male and female genotypes of Asparagus officinalis." Horticultural Science 39, No. 1 (February 16, 2012): 33–37. http://dx.doi.org/10.17221/70/2011-hortsci.

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Asparagus (Asparagus officinalis L.) plants are dioecious. All-male cultivars are desired because of their higher yields. To increase the proportion of male individuals planted in the field and expedite the breeding of all-male cultivars in asparagus, development of generally applicable molecular markers to distinguish male and female individuals is required. Bulked genomic DNA samples from ten male (XY) and ten female (XX) plants was screened with 10-bp random primers. Of the 188 primers tested, the primer T35R54 produced a 1600-bp fragment observed only in male individuals. The specificity of this T35R54-1600 marker was verified using DNA from one supermale (YY) and one female (XX) breeding line and their four F<sub>1</sub> progenies (XY). The T35R54-1600 marker fragment was observed in both supermale and all-male lines. The sequence of the T35R54 primer (5'-TTCACGGTGG-3') was absent among the sequences of primers or amplified fragments from previous studies. Therefore, this marker could be useful as a sex-related marker in future studies to increase the reliability of sex determination in asparagus.

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21

Wagh, SS, AP Suryawanshi, and DV Pawar. "RAPD Based Molecular Diversity Analysis of Different Alternaria carthami Isolates of Safflower in Maharashtra." Journal of Pure and Applied Microbiology 11, no. 3 (September 30, 2017): 1535–40. http://dx.doi.org/10.22207/jpam.11.3.38.

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22

Matsui, Toshiyuki, Yusuke Kosugi, Tomohiro Yanagi, Haruo Suzuki, Pankaj Kumar Bhow, and Sutevee Sukarakarn. "Classification of Oriental Melon by RAPD Analysis." Pakistan Journal of Biological Sciences 5, no. 2 (January 15, 2002): 208–11. http://dx.doi.org/10.3923/pjbs.2002.208.211.

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23

De la Rosa, R., A. Martin, L. Rallo, A. Angiolillo, C. Guerrero, and L. Baldoni. "RAPD AND AFLP ANALYSIS FOR OLIVE MAPPING." Acta Horticulturae, no. 586 (October 2002): 79–82. http://dx.doi.org/10.17660/actahortic.2002.586.7.

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24

Takeda, Toshihide, Takehiko Shimada, Keiichi Nomura, Takeshi Ozaki, Takashi Haji, Masami Yamaguchi, and Masao Yoshida. "Classification of Apricot Varieties by RAPD Analysis." Engei Gakkai zasshi 67, no. 1 (1998): 21–27. http://dx.doi.org/10.2503/jjshs.67.21.

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25

Liu, C., and M. Mei. "CLASSIFICATION OF LYCHEE CULTIVARS WITH RAPD ANALYSIS." Acta Horticulturae, no. 665 (January 2005): 149–60. http://dx.doi.org/10.17660/actahortic.2005.665.17.

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26

Maciuszonek, Anna, Bartosz Grajewski, and Marek Bednarczyk. "RAPD-PCR Analysis of Various Goose Populations." Folia Biologica 53, no. 1 (June 1, 2005): 83–85. http://dx.doi.org/10.3409/1734916054663384.

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27

Ranade, S. A., A. Verma, M. Gupta, and N. Kumar. "RAPD Profile Analysis of Betel Vine Cultivars." Biologia plantarum 45, no. 4 (December 1, 2002): 523–27. http://dx.doi.org/10.1023/a:1022364823330.

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28

Zheng, Z. B., H. J. Luo, S. J. Hu, S. Luo, and C. M. Liu. "IDENTIFICATION OF 'DONGYUAN' LONGAN BY RAPD ANALYSIS." Acta Horticulturae, no. 863 (May 2010): 201–6. http://dx.doi.org/10.17660/actahortic.2010.863.26.

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29

Li, Y., H. Liu, and Y. Matsubara. "CULTIVAR IDENTIFICATION BY RAPD ANALYSIS IN EPIDENDRUM." Acta Horticulturae, no. 937 (September 2012): 605–8. http://dx.doi.org/10.17660/actahortic.2012.937.73.

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30

Ortiz, A., R. Renaud, I. Calzada, and E. Ritter. "Analysis of plum cultivars with RAPD markers." Journal of Horticultural Science 72, no. 1 (January 1997): 1–9. http://dx.doi.org/10.1080/14620316.1997.11515485.

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31

Bando, Silvia Yumi, Gloria R. F. Valle, Marina B. Martinez, Luiz R. Trabulsi, and Carlos A. Moreira-Filho. "Characterization of enteroinvasiveEscherichia coliandShigellastrains by RAPD analysis." FEMS Microbiology Letters 165, no. 1 (August 1998): 159–65. http://dx.doi.org/10.1111/j.1574-6968.1998.tb13141.x.

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32

VANDER KLOET, S. P., and T. A. DICKINSON. "RAPD typification: phenetic analysis of Vaccinium inflorescences." Botanical Journal of the Linnean Society 148, no. 4 (August 2005): 445–57. http://dx.doi.org/10.1111/j.1095-8339.2005.00429.x.

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33

Makaradze, Elza, Galina Meparishvili, Natela Varshanidze, Inga Diasamidze, Ketevan Dolidze, Eteri Jakeli, and Nana Zarnadze. "RAPD-ANALYSIS OF CYCLAMEN SPP. GENOME POLYMORPHISM." CBU International Conference Proceedings 7 (September 30, 2019): 949–53. http://dx.doi.org/10.12955/cbup.v7.1483.

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Wild plants form the basis of biological resources both for Georgia and the whole world. A strategic task of any country is to preserve the biological diversity of plants. In the territory of Ajara, a large species diversity of plants grows, among which there are rare, endemic and relict plants. In particular, Cyclamen adzharicum. Modern systematics of wild plants in Georgia is based on classical methods of botany. In this regard, it is relevant to conduct genetic studies of species diversity and genetic polymorphism of species and populations using molecular genetic markers, in particular RAPD-PCR methods. The purpose of this study was to identify genetic polymorphism in Cyclamen L species using RAPD methods. As a result of the conducted research the 65 RAPD-markers in length from 150 to 1500 BP have been revealed. The number of the amplified fragments DNA varied depending on the primer from 6 (OPA-2) to 11 (OPB-4). The results of grouping Cyclamen adzharicum and C. coum samples allowed two clusters to be identified. In the first cluster were samples of three populations Cyclamen adzharicum and showed a low stubble in the intra-species variability. Cyclamen coum was attributed to the second cluster. The used primers gave the opportunity to identify polymorphism between the tested types of cyclamen.

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Shim, Young-Hun, Jung-Ho Choi, Chan-Dong Park, Chul-Joo Lim, Jung-Hee Cho, and Hong-Jin Kim. "Molecular differentiation ofPanax species by RAPD analysis." Archives of Pharmacal Research 26, no. 8 (August 2003): 601–5. http://dx.doi.org/10.1007/bf02976708.

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35

Ostrowska, Ewa, Morley Muralitharan, Stephen Chandler, Peter Volker, Sandra Hetherington, Robin Mitra, and Frank Dunshea. "Optimized conditions for rapd analysis inPinus radiata." In Vitro Cellular & Developmental Biology - Plant 34, no. 3 (July 1998): 225–30. http://dx.doi.org/10.1007/bf02822712.

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36

Hasan, Mehfuz, and Mohammad Sharif Raihan. "Genetic Variability in Bangladeshi Aromatic Rice through RAPD Analysis." Turkish Journal of Agriculture - Food Science and Technology 3, no. 3 (October 1, 2014): 107. http://dx.doi.org/10.24925/turjaf.v3i3.107-111.210.

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Genetic polymorphism and relationships among 30 commercial varieties of Bangladeshi aromatic rice (Oryza sativa L.) were established using random amplified polymorphic DNA (RAPD) primers. Out of fifty 10-mer RAPD primers screened initially, four were chosen and used in a comparative analysis of different varieties of indigenous Bangladeshi aromatic rice. Of the 33 total RAPD fragments amplified, 7 (21.21%) were found to be shared by individuals of all eight varieties. The remaining 26 fragments were found to be polymorphic (78.79%). Pair-wise estimates of similarity ranged from 0.101 to 0.911. Highest genetic diversity was determined between Radhunipagol and Dubsail varieties (0.911). The amount of genetic diversity within aromatic rice germplasm was quite high as determined by the genetic similarity coefficients between varieties. Genetic similarities obtained from RAPD data were also used to create a cluster diagram. Cluster analysis using an un-weighted pair-group method with arithmetic averages (UPGMA) was used to group the varieties and the 30 aromatic rice varieties were grouped into 6 clusters where cluster I includes the maximum number of varieties (9). Cluster VI includes minimum number of varieties (2). This Study offered a rapid and reliable method for the estimation of variability between different varieties which could be utilized by the breeders for further improvement of the local aromatic rice varieties.

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37

MIYAZAKI, Hitoshi, Aya ONODA, Hisaya TERADA, and Masahiro NAKAJIMA. "Species Identification of Pufferfish Products Using RAPD Analysis." Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi) 58, no. 2 (2017): 75–79. http://dx.doi.org/10.3358/shokueishi.58.75.

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38

Sun, Gen-Lou, Björn Salomon, and Roland von Bothmer. "Analysis of tetraploid Elymus species using wheat microsatellite markers and RAPD markers." Genome 40, no. 6 (December 1, 1997): 806–14. http://dx.doi.org/10.1139/g97-804.

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An analysis of Amplification fragment polymorphism of DNA from 27 accessions of 19 tetraploid Elymus species was carried out using 18 wheat microsatellite (WMS) primer pairs and 10 decamer primers. Ten WMS primer pairs produced multiple polymorphism on all accessions tested. Two independent phenograms, one based on WMS-PCR and one on RAPDs, separated the 19 tetraploid species into two main groups, viz., the SH genome species group and the SY genome species group. The results coincide with the genomic classification of these species and hence support previous studies showing that Elymus is not a monophyletic genus. The assays indicated that accessions within a species cluster together, which concurs with the morphological classification. Interspecific and intraspecific polymorphisms were detected by the WMS-PCR and RAPD analyses. Variation was observed among accessions of Elymus caninus. The WMS-PCR detected a much higher level of polymorphism than the RAPD analysis. WMSs seem to be more efficient markers than RAPD markers for studying the population diversity of Elymus species. The potential of cross-species amplification of microsatellite markers as an additional source for genetic analysis and applications in Elymus is discussed in the context of these results.Key words: Elymus, wheat, RAPD, phenetics, microsatellites.

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39

DeVerno, L. L., and A. Mosseler. "Genetic variation in red pine (Pinus resinosa) revealed by RAPD and RAPD-RFLP analysis." Canadian Journal of Forest Research 27, no. 8 (August 1, 1997): 1316–20. http://dx.doi.org/10.1139/x97-090.

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Swoboda, Ines, and Prem L. Bhalla. "RAPD analysis off genetic variation in the Australian fan flower, Scaevola." Genome 40, no. 5 (October 1, 1997): 600–606. http://dx.doi.org/10.1139/g97-079.

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The use of randomly amplified polymorphic DNA (RAPD) to study genetic variability in Scaevola (family Goodeniaceae), a native Australian species used in ornamental horticulture, is demonstrated. Plants of the genus Scaevola are commonly known as "fan flowers," due to the fan-like shape of the flowers. Nineteen accessions of Scaevola (12 cultivated and 7 wild) were studied using 20 random decamer arbitrary primers. Eight primers gave a distinct reproducible amplification profile of 90 scorable polymorphic fragments, enabling the differentiation of the Scaevola accessions. RAPD amplification of genomic DNA revealed a high genetic variability among the different species of Scaevola studied. Molecular markers were used to calculate the similarity coefficients, which were then used for determining genetic distances between each of the accessions. Based on genetic distances, a dendrogram was constructed. Though the dendrogram is in general agreement with the taxonomy, it also highlights discrepancies in the classification. The RAPD data showed that Scaevola aemula (series Pogogynae) is closer to Scaevola glandulifera of series Globuliferae than to the rest of members of series Pogogynae. In addition, the RAPD banding pattern of white flower S. aemula, one of the commercial cultivars, was identical to that of Scaevola albida, indicating their genetic similarity. Our study showed that there is a large genetic distance between commercial cultivars of Scaevola (Purple Fanfare, Pink Perfection, and Mauve Cluster), indicating considerable genetic variation among them. The use of RAPDs in intra- and inter-specific breeding of Scaevola is also explored.Key words: Scaevola, Australian native, RAPD, genetic distance, genetic variability.

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Samal, S., G. R. Rout, and P. C. Lenka. "Analysis of genetic relationships between populations of cashew (Anacardium occidentale L.) by using morphological characterisation and RAPD markers." Plant, Soil and Environment 49, No. 4 (December 10, 2011): 176–82. http://dx.doi.org/10.17221/4110-pse.

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In the present paper genetic relationships of twenty varieties of cashew are described on the basis of morphological characters and RAPD (Randomly Amplified Polymorphic DNA) markers. Results obtained for the phenotypic characters based on similarity coefficient were divided into four clusters with 70% similarity. By means of similarity coefficients (SG), cluster I was found to consist of twelve varieties. Cluster II consisted of a single variety, NRCC-1, cluster III consisted of six varieties and cluster IV had only one variety, Vridhachalam-2. The analysis started by using RAPD markers that allowed us to distinguish 20 varieties. A total of 80 distinct DNA fragments ranging from 0.2 to 3.0 kb were amplified by using 11 selected random 10-mer primers. Genetic similarity analysis was conducted for the presence or absence of bands in the RAPD profile. Cluster analysis clearly showed that 20 varieties of cashew grouped into two major clusters based on similarity indices. The first major cluster comprised one minor cluster. The other major cluster was divided into two sub-minor clusters, one sub-minor cluster having three varieties and the other sub-minor cluster was represented by 15 varieties. Among the 20 varieties, Ullal-3 and Dhana (H-1608) showed the highest similarity indices (87%). It was noted that Vengurla-2 and Vengurla-3 were not grouped into a single cluster but Vengurla-4 has 82% similarity to Vengurla-3. The variety Vengurla-2 has very close similarity (85%) with variety Vridhachalam-3 (M-26/2). The analysis of genetic relationships in cashew using morphological traits and RAPD banding data can be useful for plant improvement, descriptions of new varieties and also for assessment of variety purity in plant certification programmes.  

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Antolin, Michael F., Christopher F. Bosio, Julie Cotton, William Sweeney, Michael R. Strand, and William C. Black. "Intensive Linkage Mapping in a Wasp (Bracon hebetor) and a Mosquito (Aedes aegypti) With Single-Strand Conformation Polymorphism Analysis of Random Amplified Polymorphic DNA Markers." Genetics 143, no. 4 (August 1, 1996): 1727–38. http://dx.doi.org/10.1093/genetics/143.4.1727.

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Abstract The use of random amplified polymorphic DNA from the polymerase chain reaction (RAPD-PCR) allows efficient construction of saturated linkage maps. However, when analyzed by agarose gel electrophoresis, most RAPD-PCR markers segregate as dominant alleles, reducing the amount of linkage information obtained. We describe the use of single strand conformation polymorphism (SSCP) analysis of RAPD markers to generate linkage maps in a haplodiploid parasitic wasp Bracon (Habrobracon) hebetor and a diploid mosquito, Aedes aegypti. RAPD-SSCP analysis revealed segregation of codominant alleles at markers that appeared to segregate as dominant (band presence/band absence) markers or appeared invariant on agarose gels. Our SSCP protocol uses silver staining to detect DNA fractionated on large thin polyacrylamide gels and reveals more polymorphic markers than agarose gel electrophoresis. In B. hebetor, 79 markers were mapped with 12 RAPD primers in six weeks; in A. aegypti, 94 markers were mapped with 10 RAPD primers in five weeks. Forty-five percent of markers segregated as codominant loci in B. hebetor, while 11% segregated as codominant loci in A. aegypti. SSCP analysis of RAPD-PCR markers offers a rapid and inexpensive means of constructing intensive linkage maps of many species.

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Ozbey, G., A. Kilic, H. B Ertas, and A. Muz. "Random amplified polymorphic DNA (RAPD) analysis of Pasteurella multocida and Manheimia haemolytica strains isolated from cattle, sheep and goats." Veterinární Medicína 49, No. 3 (March 29, 2012): 65–69. http://dx.doi.org/10.17221/5678-vetmed.

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n this study, 30, 15 and 1 strains of <i>Pasteurella multocida</i> and 9, 8 and 6 strains of <i>Mannheimia haemolytica</i> from cattle, sheep and goats isolated in Elazig province located in the East of Turkey, respectively were typed by random amplified polymorphic DNA (RAPD) assay using a random primer (OPA-11). By RAPD assay, two and three distinct band profiles were obtained in the examination of <i>P. multocida</i> isolates from cattle and sheep, respectively. However, <i>M. haemolytica</i> isolates from cattle, sheep and goats showed only one profile and these strains were not discriminated by RAPD. This study showed that little genetic heterogeneity exists among <i>P. multocida</i> and <i>M. haemolytica</i> isolates from lungs of cattle, sheep and goats.

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Kazan, Kemal, John M. Manners, and Don F. Cameron. "Inheritance of random amplified polymorphic DNA markers in an interspecific cross in the genus Stylosanthes." Genome 36, no. 1 (February 1, 1993): 50–56. http://dx.doi.org/10.1139/g93-007.

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The inheritance of random amplified polymorphic DNA (RAPD) markers generated via the polymerase chain reaction amplification of genomic DNA sequences in an F2 family of an interspecific cross between Stylosanthes hamata and S. scabra was investigated. An initial comparison between the parental species, S. hamata cv. Verano and S. scabra cv. Fitzroy, demonstrated that 34% of detected RAPD bands were polymorphic. Of 90 primers tested, 35 showed relatively simple and reliably scorable polymorphisms and were used for segregation analysis. Sixty F2 individuals were scored for the segregation of 73 RAPD markers and 55 of these markers fit a 3:1 ratio. Segregation of eight other RAPD markers deviated significantly from a 3:1 ratio. There was no bias in the inheritance of RAPD markers regarding parental origin of the segregating RAPD markers. Linkage analysis revealed 10 linkage groups containing a total of 44 RAPD loci. Another 10 RAPD markers (7 of maternal origin) that were polymorphic between the parents did not segregate in the F2 population. One of the maternally inherited RAPD bands hybridized to chloroplast DNA. Analysis of RAPD loci by DNA hybridization indicated that mainly repeated sequences were amplified. These data indicate that RAPDs are useful genetic markers in Stylosanthes spp. and they may be suitable for genetic mapping.Key words: genetic mapping, molecular markers, polymerase chain reaction, Stylosanthes hamata, Stylosanthes scabra.

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Lee, Jae-Hong, Nam-Gil Lee, Youn-Gi Mun, Tae-Sung Jeong, Sun-Bae Kwon, Jae-Rok Kim, and Jin-Won Kim. "Genetic relationship analysis of Pleurotus pulmonarius strains using RAPD." Journal of Mushroom 13, no. 1 (March 31, 2015): 37–40. http://dx.doi.org/10.14480/jm.2015.13.1.37.

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Jeong, Jin-Woo, Cheng-Yun Jin, Mun-Ock Kim, Jae-Yun Lee, Yung-Hyun Choi, and Jae-Dong Lee. "RAPD Analysis and Cordycepin Concentration of Hybrided Cordyceps militaris Strains by Mating." Korean Journal of Mycology 37, no. 1 (June 30, 2009): 86–90. http://dx.doi.org/10.4489/kjm.2009.37.1.086.

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Wettasinghe, Ruwanthi C., and Ellen B. Peffley. "Optimizing RAPD Markers for Onion Genomic DNA Analysis." HortScience 30, no. 4 (July 1995): 877E—877. http://dx.doi.org/10.21273/hortsci.30.4.877e.

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Random amplified polymorphic DNA (RAPD) have potential as genetic markers that may facilitate selection in plant improvement. To obtain clear, reproducible, and repeatable RAPD bands, four DNA extraction protocols and two Taq polymerases were compared. DNA extraction followed modified Tai and Tanksley (PMBR), Dellaporta et al. (PMBR), and Guilllemant et al. (PMBR) protocols, and a plant tissue DNA isolation kit from Gentra Systems was used. The modified Guillemant protocol was selected because of ease of extraction and cost effectiveness. Genotypes studied were TG1015Y (Allium cepa). Polymerases compared were Taq and Taq Stoffel fragment. Results are based on separate amplifications and electrophoretic assays. PCR amplifications of Stoffel fragment produced more scorable and reproducible RAPD bands compared to bands produced using Taq polymerase.

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Das, Sasmita, Pratima Pradhan, and Ajay Kumar Sahu. "To Study the Genomic Fingerprinting of Relatedness in Strains of Bacillus Sp. by RAPD Analysis." International Journal of Trend in Scientific Research and Development Volume-3, Issue-2 (February 28, 2019): 70–75. http://dx.doi.org/10.31142/ijtsrd20284.

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Zakryś, Bożena, Robert Kucharski, and Ireneusz Moraczewski. "Genetic and morphological variability among clones of Euglena pisciformis based on RAPD and biometric analysis." Algological Studies/Archiv für Hydrobiologie, Supplement Volumes 81 (September 18, 1996): 1–21. http://dx.doi.org/10.1127/algol_stud/81/1996/1.

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Wettashinghe, Ruwanthi C., and Ellen B. Peffley. "OPTIMIZING RAPD MARKERS FOR ONION GENOMIC DNA ANALYSIS." HortScience 30, no. 3 (June 1995): 435a—435. http://dx.doi.org/10.21273/hortsci.30.3.435a.

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Random amplified polymorphic DNA (RAPD) are genetic markers that facilitate selection in plant breeding. To obtain clear reproducible, and repeatable RAPD bands, four DNA extraction protocols and two Taq polymerases were compared using thirteen TG1015Y (Allium cepa) genotypes. Protocols for DNA extraction followed those of a modified Tai and Tanksley, 1989 (PMBR); a modified Dellaporta et al., 1983 (PMBR); a modified Guillemunt et al., 1992 (PMBR); and extracted with a plant tissue DNA isolation kit from Gentra System (Minneapolis). The modified Guillemunt protocol was selected due to ease of extraction and cost effectiveness. Polymerases compared were Taq and Taq Stoffel fragment. Results are based on three separate amplifications and electrophoretic assays. PCR amplifications of Stoffel fragment produced more scorable and reproducible RAPD bands compared to bands produced using Taq polymerase.

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