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Journal articles on the topic 'Rapid microbiological method'

Author: Grafiati

Published: 4 June 2021

Last updated: 1 February 2022

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1

Raghuprakash P, Gowrav M P, Gangadharappa H V, and Hemanth Kumar S. "Rapid microbiological testing method." International Journal of Research in Pharmaceutical Sciences 11, no. 3 (2020): 3927–32. http://dx.doi.org/10.26452/ijrps.v11i3.2582.

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Classical microbiological methods currently have unacceptably long cycle times. Rapid microbiological strategies are accessible within the marketplace for about 10 years and are mostly used in the clinical laboratory and in food industries. However, their reapplication in the pharma industry has wide range of advantage. A comparison with ancient strategies to be conjointly performed. During this review, data concerning the validation of RMM strategies described within the document was given in addition as proof of the issue of validation of those strategies. A comparison with ancient strategie

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Marri, Nicla, Francesca Losito, Loris Le Boffe, et al. "Rapid Microbiological Assessment in Raw Milk: Validation of a Rapid Alternative Method for the Assessment of Microbiological Quality in Raw Milk." Foods 9, no. 9 (2020): 1186. http://dx.doi.org/10.3390/foods9091186.

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The consumption of dairy products and the dairy industry are one of the main global agri-food sectors for its size, economic importance, and level of technology. Microbiological quality of pasteurized milk or other milk products is dependent on microbiological quality of raw milk. A variety of microbiological count methods is available for monitoring the hygienic quality of raw milk. Among them, the pour plate method is the official essay for counting the number of colony-forming units in milk samples according to International Organization for Standardization (ISO) No. 4833-1:2013. The aim of

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Hübner, I., U. Obst, I. Steinmetz, and D. Bitter-Suermann. "Microbiological-Immunological Rapid Method to Detect Legionellaceae in Water." Acta hydrochimica et hydrobiologica 20, no. 4 (1992): 205–7. http://dx.doi.org/10.1002/aheh.19920200405.

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Meder, Hervé, Anne Baumstummler, Renaud Chollet, et al. "Fluorescence-Based Rapid Detection of Microbiological Contaminants in Water Samples." Scientific World Journal 2012 (2012): 1–10. http://dx.doi.org/10.1100/2012/234858.

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Microbiological contamination of process waters is a current issue for pharmaceutical industries. Traditional methods require several days to obtain results; therefore, rapid microbiological methods are widely requested to shorten time-to-result. Milliflex Quantum was developed for the rapid detection and enumeration of microorganisms in filterable samples. It combines membrane filtration to universal fluorescent staining of viable microorganisms. This new alternative method was validated using European and United States Pharmacopeia definitions, with sterile water and/or sterile water artific

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EDMISTON, ANGELA L., and SCOTT M. RUSSELL. "A Rapid Microbiological Method for Enumerating Escherichia colifrom Broiler Chicken Carcasses." Journal of Food Protection 61, no. 10 (1998): 1375–77. http://dx.doi.org/10.4315/0362-028x-61.10.1375.

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Experiments were conducted to evaluate a rapid method for enumerating Escherichia coli from broiler chicken carcasses. In three separate trials, carcasses were obtained from a commercial processing plant, temperature abused at 37°C for 0, 3, 6, 9, or 12 h, and then rinsed. E. coli were enumerated from carcass rinses using Petrifilm E. coli count plates (PC) and by placing the rinse into double-strength colifiform medium supplemented with 2% dextrose (CMD). The CMD mixture was placed into a Bactometer module and conductance was measured at 44°C. Once a detection time (DT) was recorded, the samp

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Kanda, Maki, Tomoto Kusano, Setsuko Kanai, et al. "Rapid Determination of Fluoroquinolone Residues in Honey by a Microbiological Screening Method and Liquid Chromatography." Journal of AOAC INTERNATIONAL 93, no. 4 (2010): 1331–39. http://dx.doi.org/10.1093/jaoac/93.4.1331.

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Abstract A rapid and efficient method was developed for the simultaneous determination of seven fluoroquinolone (FQ) residues: norfloxacin, ciprofloxacin, danofloxacin, enrofloxacin, orbifloxacin, sarafloxacin, and difloxacin in honey. The samples were first screened with a microbiological method by using test plates made from metal-free purified agar seeded with Bacillus subtilis BGA. When a sample was found to contain FQ residues by using the microbiological method, it was analyzed by LC with fluorescence detection (LC/FL). FQs were extracted with Na2EDTA-McIlvaine buffer and purified by a d

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INGHAM, STEVEN C., and MICHAEL W. MOODY. "Enumeration of Aerobic Plate Count and E. coli During Blue Crab Processing by Standard Methods, Petrifilm™, and Redigel™." Journal of Food Protection 53, no. 5 (1990): 423–24. http://dx.doi.org/10.4315/0362-028x-53.5.423.

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Blue crab (Callinectes sapidus) samples were collected from commercial processing plants in Louisiana and examined for microbiological quality. The major processing steps were evaluated for effects on aerobic plate count (APC) and for sources of E. coli. The reliability of simple in-plant rapid microbiological methods (Redigel™, Petrifilm™ standard plate count, and Petrifilm™ E. coli) was compared with that of standard methods. The APC increased significantly during overnight cooling prior to picking, and no consistent patterns of E. coli contamination were observed. There were no significant

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McCormick, P. J., M. J. Schoene, M. A. Dehmler, et al. "Evaluation of a Rapid Microbiological Method with a Mixed Culture Biofilm Model." PDA Journal of Pharmaceutical Science and Technology 67, no. 5 (2013): 512–32. http://dx.doi.org/10.5731/pdajpst.2013.00932.

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HORIE, Masakazu, Harumi KOBAYASHI, Rie ISHII, et al. "Simple and Rapid Microbiological Method for Determination of Residual Antibacterials in Meat." Journal of the Food Hygienic Society of Japan (Shokuhin Eiseigaku Zasshi) 49, no. 3 (2008): 168–76. http://dx.doi.org/10.3358/shokueishi.49.168.

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Mareković, Ivana, Vanda Plečko, Zagorka Boras, et al. "Evaluation of PCR as rapid microbiological method in diagnosis of pneumococcal pneumonia." Scandinavian Journal of Infectious Diseases 40, no. 10 (2008): 843–45. http://dx.doi.org/10.1080/00365540802029581.

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11

Yushina, Yuliya, Anzhelika Makhova, Elena Zayko, and Dagmara Bataeva. "Loop-mediated isothermal amplification (LAMP) for rapid detection of L. monocytogenes in meat." Potravinarstvo Slovak Journal of Food Sciences 13, no. 1 (2019): 800–805. http://dx.doi.org/10.5219/1165.

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There is a continued need to develop improved rapid methods for detection of foodborne pathogens. Rapid and sensitive methods for enumeration of Listeria monocytogenes are important for microbiological food safety testing purpose. The aim of this project was to evaluate a commercial loop-mediated isothermal amplification (LAMP) based system with bioluminescence, named as 3M™ Molecular Detection Assay (MDA), was validated for the detection of L. monocytogenes in food products with a standard GOST 32031-2012 method as reference. The results of this study revealed that a commercial LAMP-based met

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RUSSELL, SCOTT M. "A Rapid Microbiological Method for Enumeration of Pseudomonas fluorescens from Broiler Chicken Carcasses." Journal of Food Protection 60, no. 4 (1997): 385–90. http://dx.doi.org/10.4315/0362-028x-60.4.385.

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A method to selectively enumerate Pseudomonas fluorescens from fresh chicken carcasses in less than 24 h using capacitance microbiology was developed. Capacitance assays were conducted on whole-carcass rinses at 25°C using brain heart infusion broth (BHI) containing 25 μg of Irgasan per ml to obtain a detection time. The capacitance samples were spread plated on plate count agar for isolation and identification. From plates with the highest dilution, from each carcass, 4 colonies were randomly selected and identified. Seven species of bacteria including Pseudomonas fluorescens were responsible

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Reyes, Erlinda S. P., Kathleen M. Norris, Cheryl Taylor, and Donald Potts. "Comparison of Paired-Ion Liquid Chromatographic Method with AOAC Fluorometric and Microbiological Methods for Riboflavin Determination in Selected Foods." Journal of AOAC INTERNATIONAL 71, no. 1 (1988): 16–19. http://dx.doi.org/10.1093/jaoac/71.1.16.

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Abstract A paired-ion liquid chromatographic (LC) technique coupled with fluorometric detection to determine riboflavin in various food matrices is described. Chromatograms of many foods showed 2 peaks of interest due to presence of riboflavin and flavin mononucleotide (FMN). Relatively high levels of FMN were found in raw beef, corned beef, chicken liver, and canned mushrooms. When riboflavin and FMN contents were summed, LC values were comparable to those obtained by the AOAC standard procedures. The LC technique was sensitive, rapid, and simple, yielding a mean standard deviation of 3.1% wh

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Anglès d'Auriac, Marc B., Hildegarde Roberts, Terri Shaw, Reidun Sirevåg, Leonila Fajardo Hermansen, and James D. Berg. "Field Evaluation of a Semiautomated Method for Rapid and Simple Analysis of Recreational Water Microbiological Quality." Applied and Environmental Microbiology 66, no. 10 (2000): 4401–7. http://dx.doi.org/10.1128/aem.66.10.4401-4407.2000.

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ABSTRACT An early warning system using a rapid enzymatic semiautomated method suitable for fecal coliform detection in recreational waters within 8 h was developed further and evaluated in this study. This rapid method was compared to the standard method followed in the United Kingdom. We used 1,011 samples originating from 206 different locations in Wales. When we assessed the presence or absence of fecal coliforms, targeting very low levels of contamination, we obtained 83.9% agreement between the rapid method and the lauryl sulfate broth-membrane filtration technique, whereas direct confirm

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Braga, Patrícia A. C., Juliano L. Gonçalves, Juliana R. Barreiro, et al. "Rapid identification of bovine mastitis pathogens by MALDI-TOF Mass Spectrometry." Pesquisa Veterinária Brasileira 38, no. 4 (2018): 586–94. http://dx.doi.org/10.1590/1678-5150-pvb-4821.

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ABSTRACT: Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been shown to be an alternative method for identification of bacteria via their protein profile spectra, being able to identify bacteria at the genus, species and even at subspecies level. With the aim of large-scale identification of pathogens causing mastitis by this platform, a total of 305 isolates of bacteria identified from cows with subclinical mastitis were analyzed by conventional microbiological culture (MC) as well as by MALDI-TOF MS coupled with Biotyper data processing. Approx

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Stegarus, Diana Ionela, Magda Panaitescu, Ecaterina Lengyel, and Aurel Simion. "Management of Microbiological Quality of Meat in the Context of Rapid Tenderization." Management of Sustainable Development 10, no. 2 (2018): 17–20. http://dx.doi.org/10.2478/msd-2019-0003.

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Abstract This paper focuses on the study of the microbiological load of pork and beef meat, in the context of utilising fast methods of tenderizing them. The study highlights, through both classic and fast methods, the microbial load of fresh, salted and smoked pork and beef meat. It has been established that fresh pork is at around 7,2x104-8,5x104CFU/g, whereas fresh beef sits at around 2,3x103 CFU/g. For salted, smoked and classically tenderized, these values are between 3,3x104 CFU/g and 4,7x104CFU/g for pork and 6,1x102 CFU/g for beef. The method of rapid tenderizing leads to highlighting

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Katase, Mitsuru, and Kazunobu Tsumura. "Rapid Detection and Identification Systems for the Microbiological Assessment of Processed Soy Foods: A Review." Journal of Food Research 9, no. 5 (2020): 22. http://dx.doi.org/10.5539/jfr.v9n5p22.

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Plant-based diets are gaining interest in promoting physical and environmental health worldwide. The widely growing consumption of processed soy foods results in an increased demand for safe and high quality soy foods. Many of the rapid bacterial detection methods currently available are inhibited by components in the food matrixes. In recent years, high-throughput devices have been developed, which aid in the enumeration and evaluation of microorganisms in processed soy foods (automated fluorescent filter method, high-throughput identification using matrix-assisted laser desorption ionization

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Kopke, C., A. Cristovão, A. M. Prata, C. Silva Pereira, J. J. Figueiredo Marques, and M. V. San Romão. "Microbiological control of wine. The application of epifluorescence microscopy method as a rapid technique." Food Microbiology 17, no. 3 (2000): 257–60. http://dx.doi.org/10.1006/fmic.1999.0323.

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Janssen, Peter H., and Hazel Barea. "Rapid determination of amino acid concentrations in microbiological media: Evaluation of Borchers' cuprizone method." Journal of Microbiological Methods 10, no. 4 (1989): 311–16. http://dx.doi.org/10.1016/0167-7012(89)90019-5.

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Nagy, Erzsébet, Marianna Ábrók, Noémi Bartha, et al. "Special application of matrix-assisted laser desorption ionization time-of-flight mass spectrometry in clinical microbiological diagnostics." Orvosi Hetilap 155, no. 38 (2014): 1495–503. http://dx.doi.org/10.1556/oh.2014.29985.

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Matrix-assisted laser desorption ionization time-of-flight mass spectrometry as a new possibility for rapid identification of bacteria and fungi revolutionized the clinical microbiological diagnostics. It has an extreme importance in the routine microbiological laboratories, as identification of the pathogenic species rapidly will influence antibiotic selection before the final determination of antibiotic resistance of the isolate. The classical methods for identification of bacteria or fungi, based on biochemical tests, are influenced by many environmental factors. The matrix-assisted laser d

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BAUTISTA, DERRICK A., JEAN PIERRE VAILLANCOURT, ROBERT A. CLARKE, SHANE RENWICK, and MANSEL W. GRIFFITHS. "Rapid Assessment of the Microbiological Quality of Poultry Carcasses Using ATP Bioluminescence." Journal of Food Protection 58, no. 5 (1995): 551–54. http://dx.doi.org/10.4315/0362-028x-58.5.551.

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The meat industry is in need of faster and more reliable methods to determine microbial loads in food products. A rapid method (&lt;15 min) has been developed to assess the microbiological quality of chicken carcasses using the adenosine triphosphate (ATP) bioluminescence assay. The results indicate that, following modifications, the ATP bioluminescence test produced an acceptable correlation with plate counts (r = 0.85, p &lt; 0.001) and demonstrated good repeatability between replicates. It is envisaged that the modified ATP bioluminescence assay would best be used as a platform reje

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Michelutti, Luca, Michela Bulfoni, Veronica Bolzon, and Emanuele Nencioni. "Preliminary Evidence of a Molecular Detection Method to Analyze Bacterial DNA as a Quality Indicator in Cosmetics." Cosmetics 7, no. 3 (2020): 54. http://dx.doi.org/10.3390/cosmetics7030054.

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Cosmetics are a category of widely consumed and distributed products, and their manufacture is always subject to specific guidelines. Quality Control (QC) tests provide information supporting the absence of injurious organisms and regarding the microbiological stability of cosmetics. The microbiological risk analysis is typically performed using the plate count method, which is a time-consuming and operator-dependent approach. Molecular technologies allow a deeper and more sensitive testing than traditional cultures. The demand for rapid and sensitive methods is recently increasing. The aim of

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JAKŠIĆ, S., I. JAJIĆ, S. KRSTOVIĆ, and Ž. MIHALJEV. "L-lysine determination in animal feed using microbiological microtiter plate assay." Journal of the Hellenic Veterinary Medical Society 69, no. 3 (2018): 1094. http://dx.doi.org/10.12681/jhvms.18881.

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Chromatographic methods are most commonly used for the analysis of amino acids; however, there is growing need for faster, simpler and more price-effective assays. In this paper, the applicability of a rapid microbiological assay for quantification of the total content of L-lysine in feed samples was evaluated. The assay relies on the dependency of bacterial growth of Pediococcus acidilactici on the presence of L-lysine. Microbiological microtiter plate assay method for the quantitative determination of total (added and natural) L-lysine in feed samples has been verified, and parameters such a

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PRIEGO, R., L. M. MEDINA, and R. JORDANO. "Evaluation of Petrifilm Series 2000 as a Possible Rapid Method to Count Coliforms in Foods." Journal of Food Protection 63, no. 8 (2000): 1137–40. http://dx.doi.org/10.4315/0362-028x-63.8.1137.

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This research note is a preliminary comparison between the Petrifilm 2000 method and a widely used traditional enumeration method (on violet red bile agar); six batches of different foods (egg, frozen green beans, fresh sausage, a bakery product, raw minced meat, and raw milk) were studied. The reliability of the presumptive counts taken at 10, 12, and 14 h of incubation using this method was also verified by comparing the counts with the total confirmed counts at 24 h. In all the batches studied, results obtained with Petrifilm 2000 presented a close correlation to those obtained using violet

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Cieślik, Justyna, and Marta Wróblewska. "MALDI TOF MS – new possibilities in routine microbiological diagnostics." Diagnostyka Laboratoryjna 54, no. 2 (2019): 99–104. http://dx.doi.org/10.5604/01.3001.0013.7693.

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One of the problems of modern medicine is diagnosis, treatment and prophylaxis of infections caused by multidrug-resistant strains of bacteria. Fast and correct identification of these pathogens is of utmost importance, as it enables early implementation of effective therapy. Therefore, rapid, modern and affordable methods are of outstanding value as they make it possible to conduct a reliable analysis in a very short period of time. One of such techniques is matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), which in recent years is increasingly used

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Yamamoto, Célia H., and Terezinha J. A. Pinto. "Rapid Determination of Neomycin by a Microbiological Agar Diffusion Assay Using Triphenyltetrazolium Chloride." Journal of AOAC INTERNATIONAL 79, no. 2 (1996): 434–40. http://dx.doi.org/10.1093/jaoac/79.2.434.

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Abstract The standard, quantitative determination of neomycin activity by the agar diffusion method requires an 18 to 24 h incubation time. To reduce incubation time, an alternative method using triphenyltetra- zolium chloride has been developed. The inhibition zoneappears much earlier; hence, an effort was made to standardize it. This indicator develops a physical response, related to the biological one, after a determined period. A Staphylococcus epidermidis suspension inoculated into solid medium ata concentration low enough that growth is not visible can reduce the dye to formazan. There i

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LAPPALAINEN, JUHA, SATU LOIKKANEN, MARIKA HAVANA, MATTI KARP, ANNA-MAIJA SJÖBERG, and GUN WIRTANEN. "Microbial Testing Methods for Detection of Residual Cleaning Agents and Disinfectants—Prevention of ATP Bioluminescence Measurement Errors in the Food Industry." Journal of Food Protection 63, no. 2 (2000): 210–15. http://dx.doi.org/10.4315/0362-028x-63.2.210.

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The ATP luminescence measurement is based on the presence of an enzymatic reaction and may significantly be affected by cleaning agents and disinfectants. In addition, disinfectants can also reduce the activity of the luciferase enzyme and also act as ATP-releasing agents. The agents disrupt the cell walls but preserve ATP in measurable form, and therefore correlation with culture methods can be poor. Therefore, if a rapid method is used to detect ATP, a control must be used for reliable results. The possible effect of disinfectants can be eliminated with a rapid test to minimize sources of er

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Wu, Qin, Xiaoyan Gao, Muhammad Abu Bakr Shabbir, et al. "Rapid multi-residue screening of antibiotics in muscle from different animal species by microbiological inhibition method." Microchemical Journal 152 (January 2020): 104417. http://dx.doi.org/10.1016/j.microc.2019.104417.

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Reichart, O., K. Szakmár, Á. Jozwiak, J. Felföldi, and L. Baranyai. "Redox potential measurement as a rapid method for microbiological testing and its validation for coliform determination." International Journal of Food Microbiology 114, no. 2 (2007): 143–48. http://dx.doi.org/10.1016/j.ijfoodmicro.2006.08.016.

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Andrews, Wallace H. "New Trends in Food Microbiology: an AOAC INTERNATIONAL Perspective." Journal of AOAC INTERNATIONAL 80, no. 4 (1997): 908–12. http://dx.doi.org/10.1093/jaoac/80.4.908.

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Abstract The paper discusses 5 major trends in food microbiology from an AOAC INTERNATIONAL perspective. The first trend, and perhaps the one with the greatest impact on food microbiology during the past 10 years, is the introduction of the rapid test kit. The development of these kits is a result of attempts to expedite, simplify, miniaturize, and automate methods. The second trend is the introduction of new method validation programs. AOAC INTERNATIONAL offers 2 alternatives to method validation by collaborative study: the Test Kit Performance Tested Method Program and the Peer-Verified Meth

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Govari, Maria, Paschalitsa Tryfinopoulou, Foteini F. Parlapani, Ioannis S. Boziaris, Efstathios Z. Panagou, and George-John E. Nychas. "Quest of Intelligent Research Tools for Rapid Evaluation of Fish Quality: FTIR Spectroscopy and Multispectral Imaging Versus Microbiological Analysis." Foods 10, no. 2 (2021): 264. http://dx.doi.org/10.3390/foods10020264.

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The aim of the present study was to assess the microbiological quality of farmed sea bass (Dicentrarchus labrax) fillets stored under aerobic conditions and modified atmosphere packaging (MAP) (31% CO2, 23% O2, 46% Ν2,) at 0, 4, 8, and 12 °C using Fourier transform infrared (FTIR) spectroscopy and multispectral imaging (MSI) in tandem with data analytics, taking into account the results of conventional microbiological analysis. Fish samples were subjected to microbiological analysis (total viable counts (TVC), Pseudomonas spp., H2S producing bacteria, Brochothrix thermosphacta, lactic acid bac

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Bagsik, Christine T., G. C. P. Reyes, and K. E. Sulibit. "Novel automated microbiological analysis in meeting Philippine drinking water standards." Water Practice and Technology 14, no. 2 (2019): 444–48. http://dx.doi.org/10.2166/wpt.2019.033.

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Abstract The Philippines water industry is highly regulated, with monthly water quality reports required. For microbiological failures, retesting is required immediately to ensure continuous supply of potable water. Report data are generated using classical standard analytical methods, with incubation periods of 18 to 24 hours for the presumptive phase and up to an additional 48 hours for confirmation of contamination. The methods used must be accepted by the Philippine regulating bodies. Recently, instrumented microbiological techniques have been approved by the Philippine Department of Healt

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Saravia, Marta Estela, Lea Assed Bezerra Silva, Raquel Assed Bezerra Silva, et al. "Evaluation of Chair-Side Assays in High Microbiological Caries-Risk Subjects." Brazilian Dental Journal 26, no. 6 (2015): 592–95. http://dx.doi.org/10.1590/0103-6440201300389.

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The aim of this study was to evaluate the commercial chair-side assays Saliva-Check Mutans and ClinproTM Cario L-PopTM in high microbiological caries-risk dental students compared with conventional semi-quantitative colony counting culture-based technique as the reference method. Saliva samples from 93 subjects of both sexes aged 18-26 years were seeded (Köhler and Bratthall method) on plates containing SB-20M culture medium method and 12 subjects with high caries risk were selected. These 12 individuals were subjected to determination of caries risk using two commercial rapid detection chair-

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Valeriani, F., S. Giampaoli, L. Buggiotti, G. Gianfranceschi, and V. Romano Spica. "Molecular enrichment for detection of S. aureus in recreational waters." Water Science and Technology 66, no. 11 (2012): 2305–10. http://dx.doi.org/10.2166/wst.2012.435.

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The identification of rapid methods for the control of recreational water and of aquatic environments with similar characteristics is necessary to provide adequate levels of health safety for users. Molecular techniques have been proposed in recent years as a viable alternative to traditional microbiological methods, as they offer various advantages and are less time consuming than traditional tests. An innovative protocol based on molecular enrichment that allows the identification of low concentrations of Staphylococcus aureus in recreational water has been developed. The method is based on

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Meurman, J. H., J. Wahlfors, A. Korhonen, et al. "Identification of Bacteroides forsythus in Subgingival Dental Plaque with the Aid of a Rapid PCR Method." Journal of Dental Research 76, no. 7 (1997): 1376–80. http://dx.doi.org/10.1177/00220345970760070701.

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Bacteroides forsythus has been shown to be prevalent among patients with periodontitis. Conventional microbiological methods used to identify this bacterium, however, are laborious and time-consuming and are therefore not well-suited for screening purposes. We have developed a polymerase chain-reaction (PCR) method which is rapid, specific, and simple to perform and does not require other sample pre-treatment except a brief centrifugation. This method was applied to the detection of B. forsythus in subgingival plaque of 58 periodontitis patients. When compared with the results of conventional

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D'Haese, Eva, Iris Dumon, Hadewig Werbrouck, Valerie De Jonghe, and Lieve Herman. "Improved detection of Mycobacterium paratuberculosis in milk." Journal of Dairy Research 72, S1 (2005): 125–28. http://dx.doi.org/10.1017/s0022029905001226.

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At present there is no rapid microbiological method for the detection of viable Mycobacterium paratuberculosis in milk. By combining an extensive milk sample pretreatment with solid phase cytometry as the detection technique we were able to demonstrate viable mycobacterial cells in 50 ml of artificially contaminated pasteurized milk in less than one working day.

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Rubnikovich, S. P., L. N. Dedova, P. A. Semizhon, Yu L. Denisova, and O. V. Kandrukevich. "Specific characteristics of rapid diagnosis in periodontology." Proceedings of the National Academy of Sciences of Belarus, Medical series 18, no. 2 (2021): 196–203. http://dx.doi.org/10.29235/1814-6023-2021-18-2-196-203.

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Currently, the advanced studies have been justified and implemented in the Republic of Belarus that enable the production and a practical use of a domestic rapid test and allow dentists to apply microbiological diagnosis methods.The objective of the study was to develop a domestic rapid test for periodontology, indicating the procedure for preparatory clinical and laboratory measures using PCR and trypsin-like activity of periopathogenic microflora (Treponema denticola, Porphyromonas gingivalis, Bacteroides forsythus)Dental examination was performed in 60 patients with clinical signs of genera

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Frahm, E., and U. Obst. "Comparison of two rapid tests for the detection of legionellaceae in water: a microbiological-immunological method and a commercial gene-probe testkit." Water Science and Technology 31, no. 5-6 (1995): 403–6. http://dx.doi.org/10.2166/wst.1995.0648.

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Two recently developed Legionella detection tests, a microbiological-immunological method based on monoclonal antibodies (carried out as a colony-blot assay) and a commercial gene-probe testkit (the EnvironAmp Legionella Kit), are compared with the standard method. The colony-blot assay is faster than the conventional method; the gene-probe test is much faster still and is the most sensitive, but in consequence is at greater risk of false-positive results.

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Mitnick, Carole D., Richard A. White, Chunling Lu, et al. "Multidrug-resistant tuberculosis treatment failure detection depends on monitoring interval and microbiological method." European Respiratory Journal 48, no. 4 (2016): 1160–70. http://dx.doi.org/10.1183/13993003.00462-2016.

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Debate persists about monitoring method (culture or smear) and interval (monthly or less frequently) during treatment for multidrug-resistant tuberculosis (MDR-TB). We analysed existing data and estimated the effect of monitoring strategies on timing of failure detection.We identified studies reporting microbiological response to MDR-TB treatment and solicited individual patient data from authors. Frailty survival models were used to estimate pooled relative risk of failure detection in the last 12 months of treatment; hazard of failure using monthly culture was the reference.Data were obtaine

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Chun, Lindsay Y., Laura Dolle Molle, Olaf Schneewind, Dominique Missiakas, Kathleen G. Beavis, and Dimitra Skondra. "Rapid Pathogen Identification With Direct Application of MALDI-TOF Mass Spectrometry on an Endophthalmitis Vitreous Sample Without Prior Culture." Journal of VitreoRetinal Diseases 3, no. 4 (2019): 255–59. http://dx.doi.org/10.1177/2474126419847635.

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Purpose:We report a case of a 72-year-old man with bleb-related endophthalmitis (BRE) whose vitreous samples were directly analyzed with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) to rapidly identify the causative organism, whereas the results from conventional microbiological techniques were negative.Methods:We analyzed BRE vitreous samples with MALDI-TOF MS (Vitek MS, bioMérieux) for rapid pathogen identification without prior culture. Samples were also analyzed with standard microbiological methods.Results:Within 1 hour of sample acquisition,

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Gram, L., and H. Søgaard. "The potentiality of microcalorimetry as a rapid method for monitoring the microbiological quality of raw meat and fish." Thermochimica Acta 95, no. 2 (1985): 375–81. http://dx.doi.org/10.1016/0040-6031(85)85298-9.

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Siebel, E., Y. Wang, T. Egli, and F. Hammes. "Correlations between total cell concentration, total adenosine tri-phosphate concentration and heterotrophic plate counts during microbial monitoring of drinking water." Drinking Water Engineering and Science Discussions 1, no. 1 (2008): 71–86. http://dx.doi.org/10.5194/dwesd-1-71-2008.

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Abstract. The general microbial quality of drinking water is normally monitored by heterotrophic plate counts (HPC). This method has been used for more than 100 years and is recommended in drinking water guidelines. However, the HPC method is significantly handicapped because it is time-consuming and restricted to culturable bacteria. Recently, rapid and accurate detection methods have emerged, such as adenosine tri-phosphate (ATP) measurements to assess microbial activity in drinking water, and flow cytometry (FCM) to determine the total cell concentration (TCC). It is necessary and important

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Singh, Akash Ranjan, Amber Kumar, Hemant Deepak Shewade, and Bhavna Dhingra. "Poor adherence to TB diagnosis guidelines among under-five children with severe acute malnutrition in central India: A missed window of opportunity?" PLOS ONE 16, no. 3 (2021): e0248192. http://dx.doi.org/10.1371/journal.pone.0248192.

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Background In India, under-five children with Severe Acute Malnutrition (SAM) are referred to Nutritional Rehabilitation Centers (NRCs). NRCs screen the causes of SAM including tuberculosis (TB). The national TB programme recommends upfront testing with a rapid molecular test if TB is suspected in children. Objective We estimated the yield of and adherence to the TB diagnostic guidelines (clinical assessment and assessment for microbiological confirmation) among under-five children with SAM admitted at NRCs (six in district Sagar and four in district Sheopur) of Madhya Pradesh, India in 2017.

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Valente, Marta Sofia, Paulo Pedro, M. Carmen Alonso, Juan J. Borrego, and Lídia Dionísio. "Are the defined substrate-based methods adequate to determine the microbiological quality of natural recreational waters?" Journal of Water and Health 8, no. 1 (2009): 11–19. http://dx.doi.org/10.2166/wh.2009.220.

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Monitoring the microbiological quality of water used for recreational activities is very important to human public health. Although the sanitary quality of recreational marine waters could be evaluated by standard methods, they are time-consuming and need confirmation. For these reasons, faster and more sensitive methods, such as the defined substrate-based technology, have been developed. In the present work, we have compared the standard method of membrane filtration using Tergitol-TTC agar for total coliforms and Escherichia coli, and Slanetz and Bartley agar for enterococci, and the IDEXX

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dos Santos-Neto, Agenor G., Malone S. Pinheiro, Monica C. dos Santos, et al. "Development of a Manometric Monitoring Method for Early Detection of Air Microbiological Contamination in the Bloodstream." Atmosphere 12, no. 6 (2021): 702. http://dx.doi.org/10.3390/atmos12060702.

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Atmospheric air is a microbial habitat of pathogenic bioaerosols that may pose serious risks to humans. A commonly laboratory-based approach for the diagnosis of such infections in the bloodstream is the blood culture analysis. Its clinical relevance is attributed to the fact that these infections are characterized by high rates of morbidity and mortality, requiring the need for efficient methods for rapid diagnosis. For this reason, our study aimed to develop a method of manometric monitoring for the rapid detection of viable microorganisms in blood culture vials. A methodology was developed

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Pacheco, Luis G. C., Roberta R. Pena, Thiago L. P. Castro, et al. "Multiplex PCR assay for identification of Corynebacterium pseudotuberculosis from pure cultures and for rapid detection of this pathogen in clinical samples." Journal of Medical Microbiology 56, no. 4 (2007): 480–86. http://dx.doi.org/10.1099/jmm.0.46997-0.

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Corynebacterium pseudotuberculosis is the aetiological agent of caseous lymphadenitis (CLA), a debilitating disease of sheep and goats. Accurate diagnosis of CLA primarily relies on microbiological examination, followed by biochemical identification of isolates. In an effort to facilitate C. pseudotuberculosis detection, a multiplex PCR (mPCR) assay was developed targeting three genes of this bacterium: the 16S rRNA gene, rpoB and pld. This method allowed efficient identification of 40 isolates of this bacterium that had been identified previously by biochemical testing. Analysis of taxonomica

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LEE, NARI, SUNG-WOOK CHOI, HYUN-JOO CHANG, and HYANG SOOK CHUN. "Rapid Detection of Escherichia coli O157:H7 in Fresh Lettuce Based on Localized Surface Plasmon Resonance Combined with Immunomagnetic Separation." Journal of Food Protection 81, no. 5 (2018): 713–18. http://dx.doi.org/10.4315/0362-028x.jfp-17-338.

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ABSTRACT This study presents a method for rapid detection of Escherichia coli O157:H7 in fresh lettuce based on the properties of target separation and localized surface plasmon resonance of immunomagnetic nanoparticles. The multifunctional immunomagnetic nanoparticles enabling simultaneous separation and detection were prepared by synthesizing magnetic nanoparticles (ca. 10 nm in diameter) composed of an iron oxide (Fe3O4) core and gold shell and then conjugating these nanoparticles with the anti–E. coli O157:H7 antibodies. The application of multifunctional immunomagnetic nanoparticles for d

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RHEE, MIN-SUK, and DONG-HYUN KANG. "Rapid and Simple Estimation of Microbiological Quality of Raw Milk Using Chromogenic Limulus Amoebocyte Lysate Endpoint Assay." Journal of Food Protection 65, no. 9 (2002): 1447–51. http://dx.doi.org/10.4315/0362-028x-65.9.1447.

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A rapid chromogenic Limulus amoebocyte lysate (LAL) endpoint assay for the enumeration of total mesophilic microbial loads and coliforms was investigated as a means to assess the microbiological quality of raw milk. For experiment 1, raw milk samples (n = 25) were stored in a refrigerator (2 ± 2°C) and then analyzed at regular intervals (1, 5, 10, and 15 days). For experiment 2, fresh raw milk samples (n = 50) were tested to determine the utility of the LAL assay for fresh raw milk. The sample was diluted threefold in a 96-well microtiter plate with pyrogen-free water and assayed with a chromo

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Caskey, Tina, Carolyn Montei, Daniel Krause, et al. "Rapid Automated System for the Detection of Objectionable Microorganisms in Nutraceutical and Dietary Supplements." Journal of AOAC INTERNATIONAL 103, no. 1 (2020): 272–77. http://dx.doi.org/10.5740/jaoacint.19-0082.

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Abstract Background: The U.S. Pharmacopeia (USP) established microbiological procedures to evaluate nonsterile products and nutritional and dietary supplements for objectionable microorganisms of concern in the industry. A rapid method has been developed to detect the presence of microorganisms based on real-time photometric monitoring of pH, carbon dioxide, or fluorescence indicators within a novel broth/agar combination vial and accompanying reader. Objective: To validate the performance of the Neogen Rapid Microbiology System (NRMS) method against the USP reference method for the ability to

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Ivanova, L. V., T. Z. Artemova, Angelica V. Zagaynova, et al. "EVALUATION OF THE EFFECTIVENESS OF TEST-SYSTEMS FOR RAPID IMPLEMENTATION OF SANITARY-BACTERIOLOGICAL ANALYSIS OF WATER." Hygiene and sanitation 96, no. 11 (2019): 1109–14. http://dx.doi.org/10.18821/0016-9900-2017-96-11-1109-1114.

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There was executed the assessment of the method of the detection and identification of sanitary-indicative microorganisms in water samples with the use of test systems IDEXX manufactured by IDEXX Laboratories, Inc (USA), based on the use of chromogenic culture media and reagents in accordance with the methodology developed by the manufacturer. IDEXX method was found not to be inferior to the reference methods of water analysis applied in Russia and in international practice. Comparative evaluation of crops showed the consistency of results, as in the study of contaminated water with natural mi

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