Relevant bibliographies by topics / Rapid molecular diagnostics

Contents

  1. Journal articles
  2. Dissertations / Theses
  3. Books
  4. Book chapters
  5. Conference papers

Academic literature on the topic 'Rapid molecular diagnostics'

Author: Grafiati
Published: 4 June 2021
Last updated: 4 February 2022

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Journal articles on the topic "Rapid molecular diagnostics"

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Schito, Marco L., M. Patricia D’Souza, S. Michele Owen, and Michael P. Busch. "Challenges for Rapid Molecular HIV Diagnostics." Journal of Infectious Diseases 201, s1 (April 15, 2010): S1—S6. http://dx.doi.org/10.1086/650394.

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Braziel, Rita M., Margaret A. Shipp, Andrew L. Feldman, Virginia Espina, Mary Winters, Elaine S. Jaffe, Emanuel F. Petricoin, and Lance A. Liotta. "Molecular Diagnostics." Hematology 2003, no. 1 (January 1, 2003): 279–93. http://dx.doi.org/10.1182/asheducation-2003.1.279.

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Abstract It is increasingly evident that molecular diagnostics, that is, the use of diagnostic testing to understand the molecular mechanisms of an individual patient’s disease, will be pivotal in the delivery of safe and effective therapy for many diseases in the future. A huge body of new information on the genetic, genomic and proteomic profiles of different hematopoietic diseases is accumulating. This chapter focuses on new technologies and advancements in understanding the molecular basis of hematologic disorders, providing an overview of new information and its significance to patient care. In Section I, Dr. Braziel discusses the impact of new genetic information and research technologies on the actual practice of diagnostic molecular hematopathology. Recent and projected changes in methodologies and analytical strategies used by clinical molecular diagnostics laboratories for the evaluation of hematologic disorders will be discussed, and some of the challenges to clinical implementation of new molecular information and techniques will be highlighted. In Section II, Dr. Shipp provides an update on current scientific knowledge in the genomic profiling of malignant lymphomas, and describes some of the technical aspects of gene expression profiling. Analysis methods and the actual and potential clinical and therapeutic applications of information obtained from genomic profiling of malignant lymphomas are discussed. In Section III, Dr. Liotta presents an update on proteomic analysis, a new and very active area of research in hematopoietic malignancies. He describes new technologies for rapid identification of different important proteins and protein networks, and the potential therapeutic and prognostic value of the elucidation of these proteins and protein pathways in the clinical care of patients with malignant lymphomas.
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Schachter, Steven C., Denise R. Dunlap, Wilbur A. Lam, Yukari C. Manabe, Greg S. Martin, and Sally M. McFall. "Future potential of Rapid Acceleration of Diagnostics (RADx Tech) in molecular diagnostics." Expert Review of Molecular Diagnostics 21, no. 3 (March 4, 2021): 251–53. http://dx.doi.org/10.1080/14737159.2021.1898950.

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Dicks, Kristen V., and Jason E. Stout. "Molecular Diagnostics for Mycobacterium tuberculosis Infection." Annual Review of Medicine 70, no. 1 (January 27, 2019): 77–90. http://dx.doi.org/10.1146/annurev-med-040717-051502.

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Resistance to antimycobacterial drugs is a major barrier to effective treatment of Mycobacterium tuberculosis infection. Molecular diagnostic techniques based on the association between specific gene mutations and phenotypic resistance to certain drugs offer the opportunity to rapidly ascertain whether drug resistance is present and to alter treatment before further resistance develops. Current barriers to successful implementation of rapid diagnostics include imperfect knowledge regarding the full spectrum of mutations associated with resistance, limited utilization of molecular diagnostics where they are most needed, and the requirement for specialized laboratory facilities to perform molecular testing. Further understanding of genotypic–phenotypic correlates of resistance and streamlined implementation platforms will be necessary to optimize the public health impact of molecular resistance testing for M. tuberculosis.
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Ryzhkova, Olga Sergeyevna. "Rapid tests in the diagnostics of sexually transmitted infections." Journal of obstetrics and women's diseases 64, no. 1 (January 15, 2015): 34–43. http://dx.doi.org/10.17816/jowd64134-43.

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High prevalence of sexually transmitted infections (STIs) and their substantial adverse effect on reproductive health of people necessitate the development of accurate and rapid tests for their diagnostics, in particular those that can be used at point-of-care (POC). The majority of current immunological POC-tests have high specificity; however, their sensitivity is mainly suboptimal. The future of POC-diagnostics of STIs - highly sensitive and specific, robust and affordable - is seen in the development and implementation of molecular diagnostic technologies (amplification, microfluidic, biosensor).
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Lee, Youngseop, Byoung-Hoon Kang, Minhee Kang, Doo Ryeon Chung, Gwan-Su Yi, Luke P. Lee, and Ki-Hun Jeong. "Nanoplasmonic On-Chip PCR for Rapid Precision Molecular Diagnostics." ACS Applied Materials & Interfaces 12, no. 11 (February 26, 2020): 12533–40. http://dx.doi.org/10.1021/acsami.9b23591.

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Gonzalez, David, Brian A. Walker, and Gareth J. Morgan. "Ultra-Rapid, High-Throughput Molecular Diagnostics in Hemato-Oncology." Blood 106, no. 11 (November 16, 2005): 3270. http://dx.doi.org/10.1182/blood.v106.11.3270.3270.

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Abstract The clinical relevance of molecular genetic analyses in haematological malignancies is now becoming more clearly defined. Useful strategies involve the finding of mutations, differential gene expression profiles, translocations, deletions or amplifications and, more recently, epigenetic changes. Genetic abnormalities and/or gene signatures can be used to characterise a particular disease state, helping in the differential diagnosis of similar entities or, within the same malignancy genetic markers can help discriminating between subgroups with different clinical outcome. Disease-specific and/or patient-specific genetic markers can be used to monitor minimal residual disease (MRD) by real-time PCR. Unfortunately, it has been difficult so far to translate research into the clinic and set up simple, rapid and reliable tests that can be used routinely in the lab to identify and monitor these genetic abnormalities/markers in haematological patients; the main drawbacks being the labour-intensive, time-consuming nature of these methods. The new generation of ultra-fast, high-throughput automated genetic analysers and DNA/RNA extraction systems allow such analyses to be carried out on a routine basis. Routine PCRs can be performed in as little time as 20 minutes, while sequencing reactions, Real-Time PCR and high-resolution fluorescent electrophoresis can be performed in less than 1 hour. Thus, reducing significantly the processing time, increasing the cost-efficiency, and allowing urgent results to be reported on the same day. We have adapted our standard protocols for clonality detection (IGH/IGK/TCRG/TCRB), mutation analysis (IGH/IGK/IL-10/IL-6/MMSET/XBP1/FLT3), gene expression (MMSET/FGFR3/ CCND1/CCND2/CCND3/MAF/ABL/GUS/GAPDH/ACTIN)and MRD monitoring (t(9;22), t(15/17), t(12:21), t(8:21), t(1;19), inv(16;16)) to the ultra-fast, high-throughput methods without extensive further optimisation, and without loss of sensitivity or specificity. Time comparison is summarised in Table 1. In summary, with the new available generation of genetic analysers, haematology laboratories can now provide a fast and reliable diagnostic service, and rapidly incorporate new research results into molecular diagnostics. Although this technology might not be accessible to smaller labs, it should be incorporated into reference labs to improve the management of haematological patients. Table 1. Comparison of the time required to perform common molecular diagnostic analyses between the standard and fast protocols. Time (in hours) with Standard Protocols Time (in hours) with Fast Protocols Clonality Gene XP/MRD Sequencing Clonality Gene XP/MRD Sequencing *Time to process 96 samples. XP: expression; MRD: minimal residual disease; N/A: Not Available DNA/RNAextraction 0.5 - 5* 0.5 - 5* 0.5 - 5* 0.5* 0.5* 0.5* cDNA synthesis N/A 1 N/A N/A 1 N/A PCR 2.5 N/A 2.5 0.3 N/A 0.3 Real-timePCR N/A 3.5 N/A N/A 0.8 N/A SequencingReaction N/A N/A 2.5 0.8 N/A 0.8 Electrophoresis O/N N/A 3.5 0.5 N/A 0.5 TOTAL TIME 2 Days 5h to 2 Days 2 Days 2.5h 2.5h 2.5h
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Messacar, Kevin, Sarah K. Parker, James K. Todd, and Samuel R. Dominguez. "Implementation of Rapid Molecular Infectious Disease Diagnostics: the Role of Diagnostic and Antimicrobial Stewardship." Journal of Clinical Microbiology 55, no. 3 (December 28, 2016): 715–23. http://dx.doi.org/10.1128/jcm.02264-16.

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ABSTRACT New rapid molecular diagnostic technologies for infectious diseases enable expedited accurate microbiological diagnoses. However, diagnostic stewardship and antimicrobial stewardship are necessary to ensure that these technologies conserve, rather than consume, additional health care resources and optimally affect patient care. Diagnostic stewardship is needed to implement appropriate tests for the clinical setting and to direct testing toward appropriate patients. Antimicrobial stewardship is needed to ensure prompt appropriate clinical action to translate faster diagnostic test results in the laboratory into improved outcomes at the bedside. This minireview outlines the roles of diagnostic stewardship and antimicrobial stewardship in the implementation of rapid molecular infectious disease diagnostics.
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Sykes, Emma. "Tackling antimicrobial resistance through rapid diagnostics." Biochemist 40, no. 1 (February 1, 2018): 42–43. http://dx.doi.org/10.1042/bio04001042.

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Volk, Alexander E., and Christian Kubisch. "The rapid evolution of molecular genetic diagnostics in neuromuscular diseases." Current Opinion in Neurology 30, no. 5 (October 2017): 523–28. http://dx.doi.org/10.1097/wco.0000000000000478.

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Dissertations / Theses on the topic "Rapid molecular diagnostics"

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Kulkarni, Samarth. "Smart polymer nanoscale particles for rapid molecular diagnostics /." Thesis, Connect to this title online; UW restricted, 2006. http://hdl.handle.net/1773/8101.

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McIhatton, B. P. "The development of molecular diagnostics for the rapid detection of infectious agents in the immunocompromised setting." Thesis, Queen's University Belfast, 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.396901.

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Harshman, D. K., B. M. Rao, J. E. McLain, G. S. Watts, and J. Y. Yoon. "Innovative qPCR using interfacial effects to enable low threshold cycle detection and inhibition relief." AAAS, 2015. http://hdl.handle.net/10150/621255.

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UA Open Access Publishing Fund
Molecular diagnostics offers quick access to information but fails to operate at a speed required for clinical decision-making. Our novel methodology, droplet-on-thermocouple silhouette real-time polymerase chain reaction (DOTS qPCR), uses interfacial effects for droplet actuation, inhibition relief, and amplification sensing. DOTS qPCR has sample-to-answer times as short as 3 min 30 s. In infective endocarditis diagnosis, DOTS qPCR demonstrates reproducibility, differentiation of antibiotic susceptibility, subpicogram limit of detection, and thermocycling speeds of up to 28 s/cycle in the presence of tissue contaminants. Langmuir and Gibbs adsorption isotherms are used to describe the decreasing interfacial tension upon amplification. Moreover, a log-linear relationship with low threshold cycles is presented for real-time quantification by imaging the droplet-on-thermocouple silhouette with a smartphone. DOTS qPCR resolves several limitations of commercially available real-time PCR systems, which rely on fluorescence detection, have substantially higher threshold cycles, and require expensive optical components and extensive sample preparation. Due to the advantages of low threshold cycle detection, we anticipate extending this technology to biological research applications such as single cell, single nucleus, and single DNA molecule analyses. Our work is the first demonstrated use of interfacial effects for sensing reaction progress, and it will enable point-of-care molecular diagnosis of infections.
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Guerra-Moreno, Abby S. "Partial molecular characterization and development of a rapid diagnostic method for Citrus leprosis virus (CiLV) from Panama." [Gainesville, Fla.] : University of Florida, 2004. http://purl.fcla.edu/fcla/etd/UFE0004883.

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Cary, ReJeana. "Sensing of Small Molecules, Biomarkers, and Pathogens using Unique Plasmonic Assay Platforms." University of Cincinnati / OhioLINK, 2020. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1595848703283784.

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Allaouchiche, Bernard. "Méthodes de diagnostic rapide des pneumopathies acquises sous ventilation mécanique." Lyon 1, 2000. http://www.theses.fr/2000LYO1T067.

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Ndlovu, Thando. "Comparison of diagnostic tools and molecular based techniques for the rapid identification of Escherichia coli and coliforms in contaminated river water." Thesis, Cape Peninsula University of Technology, 2013. http://hdl.handle.net/20.500.11838/794.

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Thesis submitted in fulfilment of the requirements for the degree Master of Technology: Environmental Health in the Faculty of Applied Sciences at the Cape Peninsula University of Technology, 2013
Water is an important daily requirement and in a clean, pure form, it promotes health and well-being. In addition to South Africa being one of the driest countries in the world, water availability is also being compromised by massive pollution of remaining water sources. The Berg- and Plankenburg Rivers are two of the surface water sources in the Western Cape, South Africa, which are highly polluted by sewage, industrial and agricultural run-off. The current investigation was aimed at comparing diagnostic tools, which are employed by municipalities and food industries, and molecular based techniques to routinely monitor water for indicator organisms in time- and cost-effective manner. These rivers were sampled twice a month (July 2010 to January 2011) at the sites closest to the informal settlements of Kayamandi in Stellenbosch (Plankenburg River) and Mbekweni in Paarl (Berg River). The contamination levels of the two river systems were evaluated by the enumeration of Escherichia coli and coliforms using the Colilert 18® system, Membrane Filtration (MF) and Multiple Tube Fermentation (MTF) techniques. The highest faecal coliform count of 9.2 × 106 microorganisms/100 ml was obtained in weeks 21 and 28 from the Plankenburg River system by the MTF technique, while the lowest count of 1.1 × 103 microorganisms/100 ml was obtained in week one for both river systems by the MTF technique. The highest E. coli count of 1.7 × 106 microorganisms/100 ml was obtained from the Berg River system (week 9) using the MTF technique, while the lowest count of 3.6 × 102 microorganisms/100 ml was obtained by the MF technique from the Plankenburg River system. The coliform and E. coli counts obtained by the enumeration techniques thus significantly (p > 0.05) exceeded the guidelines of 2000 microorganisms/100 ml stipulated by the Department of Water Affairs and Forestry (DWAF, 1996) for water used in recreational purposes. Overall the results obtained in this study showed that the water in the Berg- and Plankenburg River systems is highly polluted, especially where these water sources are used for irrigational and recreational purposes. For the coliform and E. coli counts obtained using the three enumeration techniques, it was noted that the MTF technique was more sensitive and obtained higher counts for most of the sampling weeks. However, the media (Membrane lactose glucuronide agar) used in the MF technique also effectively recovered environmentally stressed microbial cells and it was also better for the routine selection and growth of coliforms and E. coli. While E. coli and total coliforms were detected utilising the Colilert 18® system, accurate enumeration values for these two indicator groups was not obtained for the entire sampling period for both river systems. It has previously been shown that dilutions (up to 10-3) of highly polluted waters increase the accuracy of the Colilert 18® system to enumerate colifoms and E. coli in marine waters. As the results obtained utilising the Colilert 18® system were also not comparable to the MF and MTF techniques it is recommended that highly polluted water samples be diluted to increase the accuracy of this system as a routine enumeration technique. Water samples were directly inoculated onto MacConkey, Vile Red Bile (VRB) agar and the Chromocult Coliform agar (CCA) and single colonies were inoculated onto nutrient agar. Chromocult coliform agar proved to be more sensitive than MacConkey and VRB agar for the culturing of E. coli and coliforms. Preliminary identification of these colonies was done using the RapID ONE and API 20 E systems. The most isolated Enterobacteriaceae species by both systems, included Klebsiella pneumoniae, Klebsiella oxytoca, Escherichia coli and Enterobacter cloacae in both river systems. The API 20 E system was more sensitive in the preliminary identification of the various isolates, as greater species diversity was obtained in comparison to the RapID ONE system. The Polymerase Chain Reaction (PCR) was firstly optimised using positive Enterobacteriaceae species. The optimised method was then applied to the analysis of river water samples, which were centrifuged to harvest the bacterial cells, with DNA extracted using the boiling method. The extracted DNA was amplified using conventional PCR with the aid of species specific primers. The Enterobacteriaceae species that were detected throughout the study period in both river systems include Serratia marcescens, Escherichia coli, Klebsiella pneumoniae and Bacillus cereus. Conventional PCR was the most reliable and sensitive technique to detect Enterobacteriaceae to species level in a short period of time when compared to RapID ONE and the API 20 E systems. Multiplex PCR was optimised using the positive pathogenic E. coli strains namely, Enteropathogenic E. coli (EPEC), Enteroinvasive E. coli (EIEC), Enterohaemorrhagic E. coli (EHEC) and Enteroaggregative E. coli (EAEC). It was then employed in river water sample analysis and enabled the detection of EAEC, EHEC, and EIEC strains in Berg River system, with only the EAEC detected in the Plankenburg River system. Real-time PCR was used to optimise the multiplex PCR in the amplification of E. coli strains and successfully reduced the time to obtain final results when using control organisms. Real-time PCR was found to be more sensitive and time-effective in the identification of E. coli strains, and also more pronounced DNA bands were observed in real-time PCR products compared to conventional-multiplex PCR amplicons. To sustain the services provided by the Berg- and Plankenburg Rivers in the Western Cape (South Africa), these water sources should frequently be monitored, results assessed and reported according to the practices acknowledged by responsible bodies. It is therefore recommended that the enumeration techniques be used in conjunction with the very sensitive PCR technique for the accurate detection of coliforms and E. coli in river water samples.
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Devallois, Anne. "Diagnostic rapide et epidemiologie des infections a mycobacteries : developpement et mise en place de methodes moleculaires dans la region antilles-guyane." Aix-Marseille 2, 1997. http://www.theses.fr/1997AIX20661.

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Patel, Krutarth [Verfasser], and Michael [Akademischer Betreuer] Hölscher. "Development and evaluation of urine based rapid molecular diagnostic test for pulmonary tuberculosis with potential for point of care: Cape Town Cohort / Krutarth Patel ; Betreuer: Michael Hölscher." München : Universitätsbibliothek der Ludwig-Maximilians-Universität, 2017. http://d-nb.info/1148276394/34.

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Willie, Nigani. "Plasmodium falciparum Histidine-rich Protein 2 Gene Variation and Malaria Detection in Madagascar and Papua New Guinea." Case Western Reserve University School of Graduate Studies / OhioLINK, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=case1519326080906088.

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Books on the topic "Rapid molecular diagnostics"

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Pestana, Erika, Sandor Belak, Adama Diallo, John R. Crowther, and Gerrit J. Viljoen. Early, rapid and sensitive veterinary molecular diagnostics - real time PCR applications. Dordrecht: Springer Netherlands, 2010. http://dx.doi.org/10.1007/978-90-481-3132-7.

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International Congress on Rapid Methods and Automation in Microbiology and Immunology (7th 1993 London, England). Rapid methods and automation in microbiology and immunology: RAMI-93. Andover, Hampshire: Intercept, 1994.

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Early Rapid And Sensitive Veterinary Molecular Diagnostics Real Time Pcr Applications. Springer, 2010.

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Dryden, Matthew. Near-patient testing, infection biomarkers, and rapid diagnostics. Oxford University Press, 2016. http://dx.doi.org/10.1093/med/9780198758792.003.0017.

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Treating patients with targeted antimicrobial therapy is the gold standard of care. However, empiric antimicrobial guidelines are in operation for many patients in primary or secondary care with infection. These guidelines are based on previous surveillance data and/or national recommendations, but the decision to start treatment and the choice of antimicrobial is a best-guess approach, based on clinical judgement. Microbiology laboratory results help guide and target therapies, but in general they take about 1 to 2 days to be available due to the processes involved in culturing organisms. Improvement in speed of diagnosis is the focus of research, particularly around molecular diagnostics. Near-patient testing and the use of biomarkers has been discussed as a way to tackle this issue. This chapter also considers the alternatives and future strategies that could be deployed to improve the targeted therapy of antimicrobials.
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(Editor), W. Dietmaier, C. Wittwer (Editor), and N. Sivasubramanian (Editor), eds. Rapid Cycle Real Time PCR - Methods and Applications. Springer, 2002.

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Antti, Vaheri, Tilton Richard C, Balows Albert, and International Congress on Rapid Methods and Automation in Microbiology and Immunology (6th : 1990 : Helsinki, Finland and Espoo, Finland), eds. Rapid methods and automation in microbiology and immunology. Berlin: Springer-Verlag, 1991.

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1963-, Dietmaier W., Wittwer C. 1955-, and Sivasubramanian N, eds. Rapid cycle real-time PCR: Methods and applications : genetics and oncology. Berlin: Springer, 2002.

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Steven, Specter, Bendinelli Mauro, and Friedman Herman 1931-, eds. Rapid detection of infectious agents. New York: Plenum Press, 1998.

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Specter, Steven, Herman Friedman, and Mauro Bendinelli. Rapid Detection of Infectious Agents. Springer, 2013.

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Specter, Steven. Rapid Detection of Infectious Agents. Springer, 2013.

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Book chapters on the topic "Rapid molecular diagnostics"

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Debnath, Mousumi, Godavarthi B. K. S. Prasad, and Prakash S. Bisen. "Rapid Diagnostic Methods for Biowarfare." In Molecular Diagnostics: Promises and Possibilities, 483–502. Dordrecht: Springer Netherlands, 2009. http://dx.doi.org/10.1007/978-90-481-3261-4_29.

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Schmelcher, Mathias, and Martin J. Loessner. "Use of Bacteriophage Cell Wall-Binding Proteins for Rapid Diagnostics of Listeria." In Methods in Molecular Biology, 141–56. New York, NY: Springer New York, 2014. http://dx.doi.org/10.1007/978-1-4939-0703-8_12.

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Srivastava, Ashish, Taruna Gupta, Swatantra Kumar, and Shailendra K. Saxena. "Next-Generation Rapid Advanced Molecular Diagnostics of COVID-19 by CRISPR-Cas." In Medical Virology: From Pathogenesis to Disease Control, 175–87. Singapore: Springer Singapore, 2020. http://dx.doi.org/10.1007/978-981-15-6006-4_9.

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Sachse, Konrad, and Anke Ruettger. "Rapid Microarray-Based Genotyping of Chlamydia spp. Strains from Clinical Tissue Samples." In Veterinary Infection Biology: Molecular Diagnostics and High-Throughput Strategies, 391–400. New York, NY: Springer New York, 2014. http://dx.doi.org/10.1007/978-1-4939-2004-4_28.

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Pestana, Ericka A., Sandor Belak, Adama Diallo, John R. Crowther, and Gerrit J. Viljoen. "New Trends in the Diagnosis and Molecular Epidemiology of Viral Diseases." In Early, rapid and sensitive veterinary molecular diagnostics - real time PCR applications, 47–71. Dordrecht: Springer Netherlands, 2009. http://dx.doi.org/10.1007/978-90-481-3132-7_4.

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Priye, Aashish, and Victor M. Ugaz. "Convective PCR Thermocycling with Smartphone-Based Detection: A Versatile Platform for Rapid, Inexpensive, and Robust Mobile Diagnostics." In Microfluidic Methods for Molecular Biology, 55–69. Cham: Springer International Publishing, 2016. http://dx.doi.org/10.1007/978-3-319-30019-1_3.

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Pestana, Ericka A., Sandor Belak, Adama Diallo, John R. Crowther, and Gerrit J. Viljoen. "Background." In Early, rapid and sensitive veterinary molecular diagnostics - real time PCR applications, 1–8. Dordrecht: Springer Netherlands, 2009. http://dx.doi.org/10.1007/978-90-481-3132-7_1.

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Pestana, Ericka A., Sandor Belak, Adama Diallo, John R. Crowther, and Gerrit J. Viljoen. "Traditional PCR." In Early, rapid and sensitive veterinary molecular diagnostics - real time PCR applications, 9–25. Dordrecht: Springer Netherlands, 2009. http://dx.doi.org/10.1007/978-90-481-3132-7_2.

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Pestana, Ericka A., Sandor Belak, Adama Diallo, John R. Crowther, and Gerrit J. Viljoen. "Real-Time PCR – The Basic Principles." In Early, rapid and sensitive veterinary molecular diagnostics - real time PCR applications, 27–46. Dordrecht: Springer Netherlands, 2009. http://dx.doi.org/10.1007/978-90-481-3132-7_3.

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Pestana, Ericka A., Sandor Belak, Adama Diallo, John R. Crowther, and Gerrit J. Viljoen. "Disease Diagnosis Using Real-Time PCR Specific Procedures for Important Veterinary Pathogens." In Early, rapid and sensitive veterinary molecular diagnostics - real time PCR applications, 73–234. Dordrecht: Springer Netherlands, 2009. http://dx.doi.org/10.1007/978-90-481-3132-7_5.

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Conference papers on the topic "Rapid molecular diagnostics"

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Litau, I. S., M. V. Alvarez Figueroa, A. A. Kazyulina, and L. V. Domotenko. "EVALUATION OF ANALYTICAL CHARACTERISTICS OF TB DIAGNOSTIC REAGENT KITS ON DOMESTIC CONTROL PANEL OF EXTERNAL QUALITY ASSESSMENT SAMPLES." In Molecular Diagnostics and Biosafety. Federal Budget Institute of Science 'Central Research Institute for Epidemiology', 2020. http://dx.doi.org/10.36233/978-5-9900432-9-9-216.

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The WHO tuberculosis eradication strategy includes early diagnosis of the disease through rapid diagnostic tests using molecular diagnostic techniques. For their correct use, it is necessary to improve the quality of laboratory services, including external quality assessment (EQA). In the course of the study on evaluation of analytical characteristics of TB diagnostic kits, 100% sensitivity and specificity are shown on the domestic control panel of EQA samples over a 5-year period. When determining the reproducibility of both sets of reagents, the CV did not exceed 15%.
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Klunder, Dion J. W., Maarten M. J. W. van Herpen, Aleksey Kolesnychenko, Eefje Hornix, Nicoletta Kahya, Ruth de Boer, and Henk Stapert. "A novel nano-photonics biosensor concept for rapid molecular diagnostics." In Photonics Europe, edited by Jürgen Popp, Wolfgang Drexler, Valery V. Tuchin, and Dennis L. Matthews. SPIE, 2008. http://dx.doi.org/10.1117/12.781340.

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Gurbanova, Elmira, Rafail Mehdiyev, Kai Blondal, Rasim Tahirli, Fuad Mirzayev, Doris Hillemann, Asker Ismayilov, and Alan Altraja. "Interpretation of indeterminate RIF-susceptibility results obtained by rapid molecular diagnostics test." In ERS International Congress 2016 abstracts. European Respiratory Society, 2016. http://dx.doi.org/10.1183/13993003.congress-2016.pa1907.

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Janku, Filip, Laura S. Angelo, Benoit Devogelaere, Gerald S. Fachook, Siqing Fu, Helen J. Huang, Apostolia M. Tsimberidou, et al. "Abstract 4126: BRAF mutation testing with a novel, rapid, fully-automated molecular diagnostics prototype platform." In Proceedings: AACR 104th Annual Meeting 2013; Apr 6-10, 2013; Washington, DC. American Association for Cancer Research, 2013. http://dx.doi.org/10.1158/1538-7445.am2013-4126.

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Novichikhin, D. O., A. G. Burenin, I. A. Bakhratov, and P. I. Nikitin. "Development of Rapid Multiparametric Methods of Molecular Biosensing for Early Diagnostics and Monitoring of Oncology Diseases." In 2020 International Conference Laser Optics (ICLO). IEEE, 2020. http://dx.doi.org/10.1109/iclo48556.2020.9285683.

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Huang, Helen J., Bart Claes, Gerald S. Falchook, Benoit Devogelaere, Aung Naing, Siqing Fu, Sarina Piha-Paul, et al. "Abstract C198: BRAF mutation testing of archival tumor samples with a novel, rapid, fully-automated molecular diagnostics prototype platform." In Abstracts: AACR-NCI-EORTC International Conference: Molecular Targets and Cancer Therapeutics--Oct 19-23, 2013; Boston, MA. American Association for Cancer Research, 2013. http://dx.doi.org/10.1158/1535-7163.targ-13-c198.

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Huang, Helen J., Bart Claes, Gerald S. Falchook, Apostolia M. Tsimberidou, Veronica R. Holley, Vivek Subbiah, Ralph G. Zinner, et al. "Abstract A202: BRAF mutation testing in cell-free DNA from plasma of patients with advanced cancers using a novel, rapid, automated molecular diagnostics prototype platform." In Abstracts: AACR-NCI-EORTC International Conference: Molecular Targets and Cancer Therapeutics--Oct 19-23, 2013; Boston, MA. American Association for Cancer Research, 2013. http://dx.doi.org/10.1158/1535-7163.targ-13-a202.

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Huang, Helen J., Bart Claes, Gerald S. Falchook, Veronica R. Holley, Aung Naing, Sarina A. Piha-Paul, Apostolia M. Tsimberidou, et al. "Abstract 5584: BRAF mutation testing in cell-free DNA from plasma of patients with advanced cancers using a novel, rapid, automated molecular diagnostics prototype platform (IdyllaTM)." In Proceedings: AACR Annual Meeting 2014; April 5-9, 2014; San Diego, CA. American Association for Cancer Research, 2014. http://dx.doi.org/10.1158/1538-7445.am2014-5584.

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Yeh, Hsin-Chih, Christopher M. Puleo, Yi-Ping Ho, and Tza-Huei Wang. "Towards Single-Molecule Diagnostics Using Microfluidic Manipulation and Quantum Dot Nanosensors." In ASME 2007 5th International Conference on Nanochannels, Microchannels, and Minichannels. ASMEDC, 2007. http://dx.doi.org/10.1115/icnmm2007-30213.

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Abstract:
In this report, we review several single-molecule detection (SMD) methods and newly developed nanocrystal-mediated single-fluorophore strategies for ultrasensitive and specific analysis of genomic sequences. These include techniques, such as quantum dot (QD)-mediated fluorescence resonance energy transfer (FRET) technology and dual-color fluorescence coincidence and colocalization analysis, which allow separation-free detection of low-abundance DNA sequences and mutational analysis of oncogenes. Microfluidic approaches developed for use with single-molecule detection to achieve rapid, low-volume, and quantitative analysis of nucleic acids, such as electrokinetic manipulation of single molecules and confinement of sub-nanoliter samples using microfluidic networks integrated with valves, are also discussed.
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Wang, Kuan-Chih, Steven T. Wereley, Aloke Kumar, and Han-Sheng Chuang. "Dynamic Particle Concentration for Bio-Signal Enhancement Based on an Optoelectric Microchip." In ASME 2013 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 2013. http://dx.doi.org/10.1115/imece2013-62329.

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Concentration is a vital measure to collect samples in biology. The same measure can also benefit bio-signal enhancement. Conventionally, signal detection heavily relies on the sensitivity of sensors. However, background noise usually interferes with the accuracy as the sample concentration level is below a threshold. The signals are even weaker especially in miniaturized diagnostics or point-of-care devices. Alternatively, single cell/molecule detection[1] was developed to break the engineering limitation and achieve better detectability. However, challenge concerning to single cell/molecule capturing and manipulation is a potential barrier in the field. In addition, the effectiveness of sampling remains an open quest under study. As a result, concentrating samples provides a simple, rapid, and direct means for enhancing weak bio-signal detection.
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