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Books on the topic 'Rapid molecular diagnostics'

Author: Grafiati

Published: 4 June 2021

Last updated: 4 February 2022

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1

Pestana, Erika, Sandor Belak, Adama Diallo, John R. Crowther, and Gerrit J. Viljoen. Early, rapid and sensitive veterinary molecular diagnostics - real time PCR applications. Dordrecht: Springer Netherlands, 2010. http://dx.doi.org/10.1007/978-90-481-3132-7.

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2

International Congress on Rapid Methods and Automation in Microbiology and Immunology (7th 1993 London, England). Rapid methods and automation in microbiology and immunology: RAMI-93. Andover, Hampshire: Intercept, 1994.

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3

Early Rapid And Sensitive Veterinary Molecular Diagnostics Real Time Pcr Applications. Springer, 2010.

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4

Dryden, Matthew. Near-patient testing, infection biomarkers, and rapid diagnostics. Oxford University Press, 2016. http://dx.doi.org/10.1093/med/9780198758792.003.0017.

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Treating patients with targeted antimicrobial therapy is the gold standard of care. However, empiric antimicrobial guidelines are in operation for many patients in primary or secondary care with infection. These guidelines are based on previous surveillance data and/or national recommendations, but the decision to start treatment and the choice of antimicrobial is a best-guess approach, based on clinical judgement. Microbiology laboratory results help guide and target therapies, but in general they take about 1 to 2 days to be available due to the processes involved in culturing organisms. Improvement in speed of diagnosis is the focus of research, particularly around molecular diagnostics. Near-patient testing and the use of biomarkers has been discussed as a way to tackle this issue. This chapter also considers the alternatives and future strategies that could be deployed to improve the targeted therapy of antimicrobials.

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5

(Editor), W. Dietmaier, C. Wittwer (Editor), and N. Sivasubramanian (Editor), eds. Rapid Cycle Real Time PCR - Methods and Applications. Springer, 2002.

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6

Antti, Vaheri, Tilton Richard C, Balows Albert, and International Congress on Rapid Methods and Automation in Microbiology and Immunology (6th : 1990 : Helsinki, Finland and Espoo, Finland), eds. Rapid methods and automation in microbiology and immunology. Berlin: Springer-Verlag, 1991.

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7

1963-, Dietmaier W., Wittwer C. 1955-, and Sivasubramanian N, eds. Rapid cycle real-time PCR: Methods and applications : genetics and oncology. Berlin: Springer, 2002.

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8

Steven, Specter, Bendinelli Mauro, and Friedman Herman 1931-, eds. Rapid detection of infectious agents. New York: Plenum Press, 1998.

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9

Specter, Steven, Herman Friedman, and Mauro Bendinelli. Rapid Detection of Infectious Agents. Springer, 2013.

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10

Specter, Steven. Rapid Detection of Infectious Agents. Springer, 2013.

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11

Comunicación rápida: Análisis moleculares como pruebas diagnósticas iniciales de la tuberculosis y la resistencia a la rifampicina. Organización Panamericana de la Salud, 2020. http://dx.doi.org/10.37774/9789275322383.

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Desde la aprobación por parte de la Organización Mundial de la Salud (OMS) de Xpert® MTB/RIF (Cepheid, Sunnyvale, EUA, en adelante denominado “Xpert MTB/RIF”) en el 2010, se ha generado un cúmulo considerable de evidencia sobre su uso como prueba diagnóstica inicial de la TB y la TB-RR. En los últimos meses también se han obtenido nuevos datos sobre el uso de Xpert® MTB/RIF Ultra (Cepheid, Sunnyvale, EUA, en adelante denominado “Xpert Ultra”) y sobre la última versión del sistema Truenat® MTB y MTB Plus (Molbio Diagnostics, Goa, India, en adelante denominado “Truenat”). La OMS encargó una revisión sistemática de todos los datos existentes en el 2019. Los resultados se evaluaron en la reunión del Grupo de Elaboración de Directrices (GDG) independiente, convocada por la OMS del 3 al 6 de diciembre del 2019. Las recomendaciones detalladas se publicarán en el 2020 en el marco de la actualización de las directrices consolidadas de la OMS sobre el diagnóstico de la TB. El objetivo de esta comunicación rápida es informar a los programas nacionales de tuberculosis y a otros interesados directos acerca de las principales implicaciones de la evidencia más reciente sobre el uso de análisis moleculares específicos como pruebas diagnósticas iniciales de la TB pulmonar y extrapulmonar y de la TB-RR, tanto en adultos como en niños. La actualización de las directrices consolidadas de la OMS del 2020 también incorporará las recomendaciones recientes de la OMS sobre otras pruebas rápidas como los ensayos con sondas en línea, los análisis de flujo lateral de lipoarabinomanano en orina y los análisis moleculares de amplificación isotérmica de ADN mediada por bucles. Versión oficial en español de la obra original en inglés: Molecular assays intended as initial tests for the diagnosis of pulmonary and extrapulmonary TB and rifampicin resistance in adults and children: rapid communication. Policy update. © World Health Organization 2020. ISBN: 978-92-4-000033-9.

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12

Spencer. Spencer: Rapid Methods Automation Micro Biology Immunology. Intercept, 1994.

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13

Lucas, Sebastian B. Histopathology of fungal disease. Edited by Christopher C. Kibbler, Richard Barton, Neil A. R. Gow, Susan Howell, Donna M. MacCallum, and Rohini J. Manuel. Oxford University Press, 2017. http://dx.doi.org/10.1093/med/9780198755388.003.0040.

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Histopathology has a critical role in the diagnosis of fungal infections. Often it is the first or only sample of a lesion. A rapid, confident diagnosis can significantly affect patient management. However, the morphologies of yeast and hyphae are not necessarily diagnostic at the genus or species level, and the experience of histopathologists is variable. A primary decision is whether the lesion is fungal or another infection or not infectious at all, and the next is whether the fungus is a yeast or a hyphal (mould) infection. Further histopathological genus and species discrimination can be made in many cases, but not all. Increasingly, molecular diagnostic DNA technology works effectively on formalin-fixed paraffin-embedded biopsy/autopsy material, and such information can be added to the multidisciplinary input for an optimal diagnosis.

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14

Rota, Paul A., and William J. Bellini. Zoonotic paramyxoviruses. Oxford University Press, 2011. http://dx.doi.org/10.1093/med/9780198570028.003.0047.

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Hendra virus (HeV), Nipah virus (NiV), and Menangle virus (MenV) are recently emergent paramyxoviruses that are responsible for zoonotic infections and represent potential threats to agriculture and humans. In particular, HeV and NiV cause fatal disease in animals and man, and outbreaks of NiV continue to occur almost annually in Southeast Asia. Molecular biologic studies have made substantial contributions to the characterization of these new paramyxoviruses by providing an accurate picture of their relative taxonomic positions, and molecular techniques were used to provide rapid diagnostic capabilities. In the outbreaks of NiV in Malaysia, Bangladesh, and India, molecular biological data quickly identified the etiologic agent present, and RT-PCR and serologic assays were used to rapidly confirm NiV infections in humans and animals. There has only been one report of human illness due to MenV and one study has detected an antibody response to a related rubulavirus, Tioman virus (TiV), in humans. It is interesting that all of these viruses share a common reservoir in large fruit bats. Because of their clear potential to cause severe disease in humans and animals, NiV and HeV have been designated as Class C Select Agents and have been the focus of intense study since their emergence.

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15

Izzedine, Hassan, and Victor Gueutin. Drug-induced acute tubulointerstitial nephritis. Edited by Adrian Covic. Oxford University Press, 2015. http://dx.doi.org/10.1093/med/9780199592548.003.0084.

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Drug-induced acute tubulointerstitial nephritis (ATIN) is the most common aetiology of ATIN and a potentially correctable cause of acute kidney injury (AKI). An interval of 7–10 days typically exists between drug exposure and development of AKI, but this interval can be considerably shorter following re-challenge or markedly longer with certain drugs. It occurs in an idiosyncratic and non-dose-dependent manner. Antibiotics, NSAIDs, and proton pump inhibitors are the most frequently involved agents, but the list of drugs that can induce ATIN is continuously increasing. The mechanism of renal injury is postulated to involve cell-mediated immunity, supported by the observation that T cells are the predominant cell type comprising the interstitial infiltrate. A humoral response underlies rare cases of ATIN, in which a portion of a drug molecule (i.e. methicillin) may act as a hapten, bind to the tubular basement membrane (TBM), and elicit anti-TBM antibodies. The classic symptoms of fever, rash, and arthralgia may be absent in up to two-thirds of patients. Diagnostic studies, such as urine eosinophils and renal gallium-67 scanning provide only suggestive evidence. Renal biopsy remains the gold standard for diagnosis, but it may not be required in mild cases or when clinical improvement is rapid after removal of an offending medication. Pathologic findings include interstitial inflammation, oedema, and tubulitis. The time until removal of such agents and the severity of renal biopsy findings provide the best prognostic value for the return to baseline renal function. Poor prognostic indicators are the long duration of AKI (> 3 weeks), a patient’s advanced age, and the high degree of interstitial fibrosis. Early recognition and appropriate therapy are essential to the management of drug-induced ATIN, because patients can ultimately develop chronic kidney disease. The mainstay of therapy is timely discontinuation of the causative agent, whereas controversy persists about the role of steroids.

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