Journal articles: 'Rat (wistar'

1

Li, Yan, and Kenneth E. McMartin. "Strain differences in urinary factors that promote calcium oxalate crystal formation in the kidneys of ethylene glycol-treated rats." American Journal of Physiology-Renal Physiology 296, no. 5 (May 2009): F1080—F1087. http://dx.doi.org/10.1152/ajprenal.90727.2008.

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Ethylene glycol (EG)-induced hyperoxaluria is the most commonly employed experimental regimen as an animal model of calcium oxalate (CaOx) stone formation. The variant sensitivity to CaOx among different rat strains has not been fully explored, although the Wistar rat is known to accumulate more CaOx in kidney tissue after low-dose EG exposure than in the Fischer 344 (F344) rats. Supersaturation of CaOx in tubular fluid contributes to the amount of CaOx crystal formation in the kidney. We hypothesized that the urinary supersaturation of CaOx in Wistar rats is higher than that of F344 rats, thereby allowing for greater CaOx crystal deposition in the Wistar rat. Age-matched male Wistar and F344 rats were treated with 0.75% EG or drinking water for 8 wk. Twenty-four-hour urine was collected at 0, 2, 4, 6, and 8 wk for analysis of key electrolytes to calculate the CaOx supersaturation. Plasma oxalate level was also measured. Our data confirmed the different sensitivity to renal toxicity from EG between the two rat strains (Wistar > F344). After EG treatment, the plasma oxalate level and urine oxalate excretion were markedly greater in the Wistar rats than in the F344 rats, while urine calcium was slightly decreased in Wistars. Thus, the CaOx supersaturation in urine of Wistar rats was higher, which led to a greater crystal deposition in kidney in Wistar rats. These studies suggest that during EG treatment, changes in urine electrolytes and in CaOx supersaturation occur to a greater extent in the Wistar rat, in agreement with its greater sensitivity to EG toxicity.

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2

Martins, A. R., G. S. S. Matias, V. F. Batista, M. A. Miglino, and P. Fratini. "Wistar rat dermis recellularization." Research in Veterinary Science 131 (August 2020): 222–31. http://dx.doi.org/10.1016/j.rvsc.2020.05.005.

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3

Kava, R., R. G. Peterson, D. B. West, and M. R. C. Greenwood. "Wistar Diabetic Fatty Rat." ILAR Journal 32, no. 3 (January 1, 1990): 9–13. http://dx.doi.org/10.1093/ilar.32.3.9.

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4

Wilby, Owen K., Karen Critchell, and Debbie Coulby. "The Wistar rat chondrodystrophy syndrome." Reproductive Toxicology 32, no. 2 (September 2011): 177–78. http://dx.doi.org/10.1016/j.reprotox.2011.06.113.

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5

FITZGIBBON, WAYNE R., EDDIE L. GREENE, JASJIT S. GREWAL, FLORENCE N. HUTCHISON, SALLY E. SELF, SAJATA Y. LATTEN, and MICHAEL E. ULLIAN. "Resistance to Remnant Nephropathy in the Wistar-Furth Rat." Journal of the American Society of Nephrology 10, no. 4 (April 1999): 814–21. http://dx.doi.org/10.1681/asn.v104814.

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Abstract. The Wistar-Furth rat, an inbred strain resistant to actions of mineralocorticoids, was used to study the concept that mineralocorticoids contribute to progressive renal injury. It was postulated that if chronic nephropathy depends on aldosterone and if Wistar-Furth rats are resistant to aldosterone, remnant nephropathy would be attenuated in Wistar-Furth rats. Wistar-Furth rats and control Wistar rats were subjected to 5/6 nephrectomy or a sham procedure and then followed for 4 wk. Renal ablation resulted in hypertension at 4 wk in both strains (164 ± 5 [Wistar-Furth] versus 184 ± 7 [Wistar] mmHg mean arterial pressure), with sham animals remaining normotensive (134 ± 6 mmHg). Renal damage in response to 5/6 nephrectomy was greatly decreased in Wistar-Furth rats compared with Wistar rats. Albuminuria was markedly less in Wistar-Furth rats (12.7 ± 4.2 [Wistar-Furth] versus 97.4 ± 22.6 [Wistar] mg/d per 100 g body wt, P < 0.01). Glomerular damage, consisting of mesangial proliferation, mesangial lysis, and segmental necrosis, was observed in 42% of glomeruli from Wistar rats but in 0% of glomeruli from Wistar-Furth rats (P < 0.01). To address the possibility that higher BP in partially nephrectomized Wistar rats mediated the greater renal damage, the study was repeated, with Wistar rats (not Wistar-Furth rats) being treated with a hydralazine-reserpine-hydrochlorothiazide regimen. Although this antihypertensive regimen equalized BP (conscious systolic) (144 ± 8 mmHg [Wistar] versus 157 ± 7 mmHg [Wistar-Furth] at 4 wk), albuminuria remained more than 10-fold greater in Wistar rats. In summary, renal damage upon 5/6 nephrectomy was markedly reduced in Wistar-Furth rats, a finding not attributable to reduced systemic BP. Since Wistar-Furth rats have been shown previously to be resistant to the actions of mineralocorticoids, the data from the present study support the hypothesis that aldosterone mediates, at least in part, the renal injury attendant to renal mass reduction.

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6

Silva, Letícia Maria, Fernanda Bresolin, Jane Prado, Leonardo Grouschekei, Andressa Zanon, Antonio Leis-Filho, Juliana Rozolen, Carlos Fonseca-Alves, and Fabiana Elias. "Primary hepatic fibrosarcoma in a Wistar rat (Rattus norvegicus)." Brazilian Journal of Veterinary Pathology 13, no. 1 (March 31, 2020): 21–25. http://dx.doi.org/10.24070/bjvp.1983-0246.v13i1p21-25.

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7

Li, Yan, Marie C. McLaren, and Kenneth E. McMartin. "Involvement of urinary proteins in the rat strain difference in sensitivity to ethylene glycol-induced renal toxicity." American Journal of Physiology-Renal Physiology 299, no. 3 (September 2010): F605—F615. http://dx.doi.org/10.1152/ajprenal.00419.2009.

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Ethylene glycol (EG) exposure is a common model for kidney stones, because animals accumulate calcium oxalate monohydrate (COM) in kidneys. Wistar rats are more sensitive to EG than Fischer 344 (F344) rats, with greater COM deposition in kidneys. The mechanisms by which COM accumulates differently among strains are poorly understood. Urinary proteins inhibit COM adhesion to renal cells, which could alter COM deposition in kidneys. We hypothesize that COM accumulates more in Wistar rat kidneys because of lower levels of inhibitory proteins in urine. Wistar and F344 rats were treated with 0.75% EG in drinking water for 8 wk. Twenty-four-hour urine was collected every 2 wk for analysis of urinary proteins. Similar studies were conducted for 2 wk using 2% hydroxyproline (HP) as an alternative oxalate source. Total urinary protein was higher in F344 than Wistar rats at all times. Tamm-Horsfall protein was not different between strains. Osteopontin (OPN) levels in Wistar urine and kidney tissue were higher and were further increased by EG treatment. This increase in OPN occurred before renal COM accumulation. Untreated F344 rats showed greater CD45 and ED-1 staining in kidneys than untreated Wistars; in contrast, EG treatment increased CD45 and ED-1 staining in Wistars more than in F344 rats, indicating macrophage infiltration. This increase occurred in parallel with the increase in OPN and before COM accumulation. Like EG, HP induced markedly greater oxalate concentrations in the plasma and urine of Wistar rats compared with F344 rats. These results suggest that OPN upregulation and macrophage infiltration do not completely protect against COM accumulation and may be a response to crystal retention. Because the two oxalate precursors, EG and HP, produced similar elevations of oxalate, the strain difference in COM accumulation may result more so from metabolic differences between strains than from differences in urinary proteins or inflammatory responses.

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8

Chen, Tao, Ke Chen, Shaung Qiu, and Peter C. Mann. "Spontaneous Cholangiofibrosis in a Wistar Rat." Toxicologic Pathology 47, no. 4 (April 15, 2019): 556–60. http://dx.doi.org/10.1177/0192623319842549.

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In a 2-year carcinogenicity study, we identified a spontaneous cholangiofibrosis in a control male Wistar rat. This lesion has long been considered as a compound-related change, with no spontaneous cases reported in the Wistar rat. In addition to routine hematoxylin and eosin stains evaluation, we applied Masson’s trichrome staining, Alcian blue-periodic acid–Schiff staining, and OV-6 immunohistochemistry staining. The special staining demonstrated the fibrous component in the interstitium and intestinal metaplasia of the epithelium (presence of goblet cells), while the positive anti-OV-6 reaction indicated the bile duct origin of the epithelium. These results help to confirm the diagnosis of cholangiofibrosis in this case. We report this rare case to alert pathologists that spontaneous cholangiofibrosis does occur in Wistar rats.

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9

Khosho, F. K., R. C. Kaufmann, and K. S. Amankwah. "Cervical and vaginal surface epithelial changes in persistent estrous, chemically diabetic BB wistar rats observed by scanning electron microscope." Proceedings, annual meeting, Electron Microscopy Society of America 44 (August 1986): 250–51. http://dx.doi.org/10.1017/s0424820100142876.

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Studies of the effects of diabetes on reproduction in the female BB Wistar rat are not available in the literature. Male reproduction has been studied in these spontaneously-diabetic BB Wistar rats, and abnormalities of the reproductive tract have been found. This investigation, therefore, compared the ultrastructure of the lower reproductive tract of the chemically-diabetic female BB Wistar rat which has developed persistent estrous(PE) to normalcycling controls.

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10

Teredesai, A., and T. Wohrmann. "Endocardial Schwannomas in the Wistar Rat." Journal of Veterinary Medicine Series A 52, no. 8 (October 2005): 403–6. http://dx.doi.org/10.1111/j.1439-0442.2005.00750.x.

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11

Murkunde, Yogeshkumar V., Ponnusamy Kalaiselvan, Subramaniyan Vijayakumar, Kuppusamy Hemalatha, Robert R. Maronpot, Ronald A. Herbert, and Monique Y. Wells. "Brain lesion in a Wistar rat." Lab Animal 37, no. 9 (September 2008): 401. http://dx.doi.org/10.1038/laban0908-401.

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12

Fitzgerald, James E. "Ameloblastic Odontoma in the Wistar Rat." Toxicologic Pathology 15, no. 4 (June 1987): 479–81. http://dx.doi.org/10.1177/019262338701500414.

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13

Ernst, Heinrich, and Dumitru Mirea. "Ameloblastoma in a female Wistar rat." Experimental and Toxicologic Pathology 47, no. 5 (January 1995): 335–40. http://dx.doi.org/10.1016/s0940-2993(11)80343-x.

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14

Okazaki, Yoshimasa, Junko Matsumoto, Osamu Katsuta, and Minoru Tsuchitani. "Dilated cardiomyopathy in a wistar rat." Journal of Toxicologic Pathology 8, no. 2 (1995): 155–59. http://dx.doi.org/10.1293/tox.8.155.

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15

Ewuola, E. O., and P. C. Emerue. "Mitigating dietary aflatoxins in Wistar rat using selected phyto-antioxidant sources." Nigerian Journal of Animal Production 48, no. 2 (March 2, 2021): 38–52. http://dx.doi.org/10.51791/njap.v48i2.2931.

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This study was conducted to investigate the mitigation of dietary aflatoxin in Wistar rats using extract from selected phyto-antioxidant sources. A total of one hundred and twenty Wistar rats weighing between 180-190g (80 females and 40 males) at six weeks old were allotted to eight treatments with 15 rats per treatment (10 females and 5 males), in a completely randomized design. The treatments were Treatment 1 (Normal diet + no extract and no aflatoxin), Treatment 2 (Aflatoxin contaminated diet + no extract), Treatment 3 (Aflatoxin contaminated diet + 100mg/kg BW Carrot extract), Treatment 4 (Aflatoxin contaminated diet + 200mg/kg BW Carrot extract), Treatment 5 (Aflatoxin contaminated diet + 100mg/kg BW Ginger extract), Treatment 6 (Aflatoxin contaminated diet + 200mg/kg BW Ginger extract), Treatment 7 (Aflatoxin contaminated diet +100mg/kg BW Garlic extract), Treatment 8 (Aflatoxin contaminated diet+ 200mg/kg BW Garlic extract). Body weight gain and feed conversion ratio of Wister rats (T8) administered 200 mg/kg BW was significant (p<0.05), with higher weight gain in male (206.00g) and female (199.70g). However, eviscerated and organs weights of both male and female Wistar rats was similar (p>0.05) across the treatments. Haematological and serum biochemical indices among the treatments was not significant (p>0.05), except for the globulin in male Wistar rats that differed significantly (p<0.05) with the value (5.00g/dL) being higher in T7. In conclusion, 200mg/kg body weight of garlic extract improved growth rate of Wistar rats, without any deleterious effect on haematological and serum biochemical parameters. Therefore, 200mg/kg body weight garlic extract mitigated the adverse effect of aflatoxin contaminated feed in Wistar rats. Cette étude a été menée pour étudier l'atténuation de l'aflatoxine diététique chez les rats de 'Wistar' tout en employant l'extrait des sources phyto-antioxydantes choisies. Un total de cent vingt rats Wistar pesant entre 180 et 190 g (80 femelles et 40 mâles) à l'âge de six semaines ont été attribués à huit traitements avec 15 rats par traitement (10 femelles et 5 mâles), dans une conception complètement randomisée. Les traitements étaient le traitement 1 (régime normal + aucun extrait et aucune aflatoxine), traitement 2 (régime contaminé par l'aflatoxine + aucun extrait), traitement 3 (régime contaminé par l'aflatoxine + extrait de carotte BW de 100mg/kg), traitement 4 (régime contaminé par l'aflatoxine + extrait de carotte BW de 200mg/kg), traitement 5 (Aflatox alimentation contaminée par l'aflatoxine + extrait de gingembre BW 100mg/kg), Traitement 6 (régime contaminé à l'aflatoxine + extrait de gingembre BW 200mg/kg), Traitement 7 (régime contaminé par l'aflatoxine +100mg/kg extrait d'ail BW), Traitement 8 (régime contaminé à l'aflatoxine+ 200mg/kg extrait d'ail BW). Le gain de poids corporel et le rapport de conversion des aliments pour animaux des rats wisters (T8) administrés 200 mg/kg BW étaient significatifs (p<0.05), avec un gain de poids plus élevé chez les rats Wistar mâles (206.00 g) et femelles (199.70 g). Cependant, les poids éviscérés et organes des rats Wistar mâles et femelles étaient similaires (p>0.05) à travers les traitements. Les indices biochimiques hématologiques et sériques parmi les traitements n'étaient pas significatifs (p>0.05), à l'exception de la globuline chez les rats wistar mâles qui différait considérablement (p<0.05) avec la valeur (5.00 g/dL) étant plus élevée dans T7. En conclusion, le poids corporel de 200mg/kg de l'extrait d'ail a amélioré le taux de croissance des rats de Wistar, sans n'importe quel effet délétère sur les paramètres biochimiques hématologiques et sériques. Par conséquent, l'extrait d'ail de poids corporel de 200mg/kg a atténué l'effet défavorable de l'alimentation contaminée d'aflatoxin chez les rats de Wistar.

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16

Kaiser, Michael A., David Lodwick, and Nilesh J. Samani. "The Rat SA Gene Shows Genotype-Dependent Tissue-Specific Expression." Clinical Science 87, no. 1 (July 1, 1994): 1–4. http://dx.doi.org/10.1042/cs0870001.

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1. SA is a recently identified gene implicated in blood pressure regulation in rodent models of genetic hypertension. In this study we have examined, by Northern blotting, its expression in tissues of the spontaneously hypertensive rat, the Wistar-Kyoto rat and F2 rats, derived from a cross of the spontaneously hypertensive rat with the Wistar-Kyoto rat. 2. We demonstrate that the gene is expressed in a tissue-specific manner. Expression was detected in four sites: kidney, liver, brain and testes. 3. In the kidney and liver expression was higher in the spontaneously hypertensive rat than in the Wistar-Kyoto rat, whereas in the brain and testes the pattern was reversed. 4. In the F2 rats, the levels of SA mRNA in the liver, brain and testes were found to be primarily determined by the genotype at the SA gene locus. 5. The findings suggest the presence of strain-specific cis- and more than one tissue-specific trans-acting factors regulating the expression of the rat SA gene.

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MATOSZ, Bianca, Flavia RUXANDA, Adrian Florin GAL, Vlad Emil LUCA, and Viorel MICLĂUȘ. "Intraspecific Differences Regarding Granular Polymorphism in Granular Ducts’ Cells in Rats’ Mandibular Gland." Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Veterinary Medicine 75, no. 1 (May 19, 2018): 1. http://dx.doi.org/10.15835/buasvmcn-vm:000417.

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Mandibular gland ducts’ system in rodents consist of intralobular ducts (intercalated, granular, striated) and interlobular one (main excretory duct). Granular ducts are located between intercalated and striated ducts, being present only in mandibular gland of the mouse, rat, hamster and gerbil. The biological material used for this study was represented by two strains from the same species, three Wistar rats and three Brown Norway rats. After the animals were euthanized, the mandibular glands were harvested and then processed for histological investigations. The tissue fragments were sectioned at 5μm thickness and then stained the sections using Tricrom-Goldners method. Our results emphasize that the granular ducts are well developed; regarding the shape, they are convoluted in both Wistar and Brown Norway rats, without any significant differences between the two strains. In Wistar rat, the granules in granular ducts cells are small to medium in size, with discrete polymorphism. In Brown Norway rat, the cytoplasm is loaded with granules as in Wistar rat, but these are several times larger and more polymorphic.

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18

Котеров, А., A. Koterov, Л. Ушенкова, L. Ushenkova, Э. Зубенкова, E. Zubenkova, А. Вайнсон, et al. "Dependence of Body Weight on Age for Random-Bred Albino Rat and for Eight Lines of Laboratory Rat: Synthetic Studies of Data from Experimental Works and Nurseries in Aspect of the Relationship with Radiosensitivity. Some Characteristics of Rat Species." Medical Radiology and radiation safety 63, no. 2 (April 5, 2018): 15–17. http://dx.doi.org/10.12737/article_5ac6190e95da25.42157674.

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For random-bred albino rat and for eight most known rat lines (Wistar, Wistar Hannover, Wistar Kyoto, Sprague Dawley, Lewis, Fisher 344, Lister and Long-Evans) a brief review of the origins and features was made, and data on the age–weight dependences in norm obtained from experimental works and presented in the materials of firms and nurseries were analysed. The data extracted from the sources by digitizing the original curves or taken from there from the tables were combined (Mean ± 95% Confidence Intervals), and the values were compared in parallel along the Student’s t-test and the Mann–Whitney U-test. For half the rat lines (males and females) it was found that the body weight growth in works and nurseries does not coincide (statistically significant or in the form of distinct trends), and the discrepancy can began either from a certain time moment (Wistar Hannover, Sprague Dawley), or almost immediately after birth (Lewis, Long-Evans). The detected phenomenon has practical significance for the object selection for radiosensitivity investigation. Differences in age at the same weight of animals in the experiment and in nurseries can cause errors in background radioresistance. A review of the studies on dependence of the radiosensitivity on the age of irradiated rats was performed with the reproduction of a number of published data in a graphic form and it was concluded that a mistake in the age of rats even for a few weeks can strongly affect the radiosensitivity. It is noted that the importance of taking into account the body mass index is due to the dependence on it of the mass of internal organs, the magnitude of which is affected, among other things, on the results of internal dosimetry. Distribution by growth intensity (an age of achievement of weight 200 g) for males is follows: Wistar > Sprague-Dawley = Lister > Long-Evans (from nurseries) > Wistar Hannover > Lewis > Wistar Kyoto > Fisher 344 > Long-Evans (from works) > Wistar from 1906–1932 > random-bred albino. As a result of the study, standard, tabular growth curves for random-bred rat and eight mentioned rat strains obtained by combining and statistical processing of data from all available sources were also presented. This material continues the traditions of Donaldson’s Tables (H.H. Donaldson, 1915) and the growth standards for laboratory animal lines in work of S.M. Poiley (1972). The report of the individual data by some characteristics of a rat species is presented: average life expectancy, age and weight for various physiological periods of the development, and also a certain ‘standard’ weight for a rat as a species.

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19

Nair, Dr Neena. "Impact of Nickel Chloride on Wistar Rat Epididymis: A Biochemical Study." Indian Journal of Applied Research 4, no. 6 (October 1, 2011): 528–30. http://dx.doi.org/10.15373/2249555x/june2014/165.

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20

Pace, V., S. Scarsella, and E. Perentes. "Parathyroid Gland Carcinoma in a Wistar Rat." Veterinary Pathology 40, no. 2 (March 2003): 203–6. http://dx.doi.org/10.1354/vp.40-2-203.

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An anaplastic carcinoma was found in one of the two parathyroids of a 2-year-old male Wistar rat, which was sacrificed at the end of a carcinogenicity study. Morphologically, it was characterized by the presence of nodular areas of pleomorphic and dense cells with numerous atypical mitoses and large regions of smaller and dark monomorphic cells devoid of mitoses and forming small cystic spaces. Local invasion of the capsule and pronounced compression of the parenchyma of the thyroid gland were observed. Immunohistochemically, the tumor was markedly positive for the parathyroid hormone and negative for the thyroid transcription factor. The proliferative activity was assessed by immunostaining the endogenous cell proliferation associated-antigen Ki-67, and the proliferating cell nuclear antigen. The diagnosis of carcinoma of the parathyroid was made on the basis of microscopic and immunohistochemical findings.

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21

Möller, Richard, Noelia Vazquez, Diana Teliz, and Virginia Méndez. "Digestive Peritoneum in Wistar Rat (Rattus norvegicus)." International Journal of Morphology 31, no. 1 (March 2013): 128–30. http://dx.doi.org/10.4067/s0717-95022013000100020.

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22

TANAKA, Toshimitsu, Kosei GOTOH, Ryoh'ichi SATOH, Mizuho INAZU, and Takayoshi KOBAYASHI. "Spontaneous meningioma in a young Wistar rat." Japanese Journal of Veterinary Science 52, no. 5 (1990): 1103–5. http://dx.doi.org/10.1292/jvms1939.52.1103.

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23

Borràs, M. "A supernumerary forelimb in a Wistar rat." Laboratory Animals 21, no. 3 (July 1, 1987): 223–25. http://dx.doi.org/10.1258/002367787781268800.

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Solberg, Leah C., Susan Losee Olson, Fred W. Turek, and Eva Redei. "Altered hormone levels and circadian rhythm of activity in the WKY rat, a putative animal model of depression." American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 281, no. 3 (September 1, 2001): R786—R794. http://dx.doi.org/10.1152/ajpregu.2001.281.3.r786.

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The Wistar Kyoto (WKY) rat is hyperreactive to stress and exhibits depressive-like behavior in several standard behavioral tests. Because patients with depressive disorders often exhibit disruptions in the circadian rhythm of activity, as well as altered secretory patterns of the hypothalamic-pituitary-adrenal and hypothalamic-pituitary-thyroid hormones, we tested the hypothesis that these phenomena occur in the WKY rat. Plasma ACTH and corticosterone levels remained significantly higher after the diurnal peak for several hours in WKY rats relative to Wistar rats. Also, plasma levels of thyroid-stimulating hormone were significantly higher in WKY relative to Wistar rats across the 24-h period, despite normal or slightly higher levels of 3,5,3′-triiodothyronine. In addition, under constant darkness conditions, WKY rats exhibited a shorter free running period and a decreased response to a phase-delaying light pulse compared with Wistar rats. In several ways these results are similar to those seen in other animal models of depression as well as in depressed humans, suggesting that the WKY rat could be used to investigate the genetic basis for these abnormalities.

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Dechandt, Carlos Roberto Porto, Tatiane M. Vicentini, Guilherme Pauperio Lanfredi, Rui M. P. Silva-Jr., Enilza Maria Espreafico, José A. Cortes de Oliveira, Vitor Marcel Faça, Norberto Garcia-Cairasco, and Luciane Carla Alberici. "The highly efficient powerhouse in the Wistar audiogenic rat, an epileptic rat strain." American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 316, no. 3 (March 1, 2019): R243—R254. http://dx.doi.org/10.1152/ajpregu.00254.2018.

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The Wistar audiogenic rat (WAR) is an animal model of tonic-clonic epileptic seizures, developed after genetic selection by sister × brother inbreeding of Wistar rats susceptible to sound stimuli. Although metabolic changes have been described in this strain, nothing is known about its mitochondrial metabolism. Here, we addressed mitochondrial aspects of oxidative phosphorylation, oxidative stress, biogenesis, and dynamics in liver, skeletal muscle, and heart of male WARs and correlating them with physiological aspects of body metabolism. The results showed higher mitochondrial content, respiration rates in phosphorylation and noncoupled states, and H2O2 production in WARs. Liver presented higher content of peroxisome proliferator-activated receptor-γ coactivator 1α (PGC1α) and mammalian target of rapamycin, proteins related to mitochondrial biogenesis. In agreement, isolated liver mitochondria from WARs showed higher respiration rates in phosphorylation state and ADP-to-O ratio, as well as higher content of proteins related to electron transport chain ATP synthase, TCA cycle, and mitochondrial fusion and fission compared with their Wistar counterparts. Mitochondria with higher area and perimeter and more variable shapes were found in liver and soleus from WARs in addition to lower reduced-to-oxidized glutathione ratio. In vivo, WARs demonstrated lower body mass and energy expenditure but higher food and water intake and amino acid oxidation. When exposed to a running test, WARs reached higher speed and resisted for a longer time and distance than their Wistar controls. In conclusion, the WAR strain has mitochondrial changes in liver, skeletal muscle, and heart that improve its mitochondrial capacity of ATP production, making it an excellent rat model to study PGC1α overexpression and mitochondrial function in different physiological conditions or facing pathological challenges.

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Burton, G. A., J. Haylor, and A. de Jonge. "Renal sensitivity to endothelium-derived-relaxing-factor-mediated vasodilatation in the spontaneously hypertensive rat." Clinical Science 80, no. 5 (May 1, 1991): 435–41. http://dx.doi.org/10.1042/cs0800435.

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1. The sensitivity of the kidney to endothelium-derived-relaxing-factor-mediated vasodilatation has been investigated in the spontaneously hypertensive rat and the Wistar-Kyoto normotensive rat using an isolated perfused rat kidney model. 2. No difference in the slope, ED50 or maximum of the concentration-response curves for the endothelial-dependent vasodilators A23187, a calcium ionophore, and acetylcholine could be demonstrated between kidneys obtained from the spontaneously hypertensive and the Wistar-Kyoto normotensive rats. 3. No difference in the slope or the ED50 of the concentration-response curve for the endothelial-independent vasodilators, atrial natriuretic factor and sodium nitroprusside, could be demonstrated between kidneys obtained from the spontaneously hypertensive and the Wistar-Kyoto normotensive rats. However, in the spontaneously hypertensive rats, the maximum vasodilator response to atrial natriuretic factor, but not to sodium nitroprusside, was increased. 4. The perfused kidney from the spontaneously hypertensive rat also showed an increase in the maximum but not in the slope or ED50 of the concentration-response curve for vasoconstriction induced by the α1-adrenoceptor agonist methoxamine. 5. The involvement of endothelium-derived relaxing factor in mediating the renal vasodilator response to A23187 and acetylcholine was confirmed in experiments performed in perfused kidneys obtained from normotensive Wistar rats. 6. It is concluded that the sensitivity of the kidney to endothelium-derived-relaxing-factor-mediated vasodilatation is not modified in the spontaneously hypertensive rat. This does not, however, exclude a role for the synthesis of endothelium-derived relaxing factor in the maintenance of blood pressure in the spontaneously hypertensive rat.

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27

Green, M. D., C. N. Falany, R. B. Kirkpatrick, and T. R. Tephly. "Strain differences in purified rat hepatic 3 α-hydroxysteroid UDP-glucuronosyltransferase." Biochemical Journal 230, no. 2 (September 1, 1985): 403–9. http://dx.doi.org/10.1042/bj2300403.

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Qualitative and quantitative differences of purified hepatic 3 α-hydroxysteroid UDP-glucuronosyltransferase were investigated in Wistar and Sprague-Dawley rats. Individual differences in the glucuronidation rate of androsterone and chenodeoxycholic acid were observed in hepatic microsomal fractions from Wistar but not Sprague-Dawley rats. No individual variation was observed in the glucuronidation of testosterone, p-nitrophenol or oestrone. The 3 α-hydroxysteroid UDP-glucuronosyltransferases from livers of Wistar and Sprague-Dawley rats were isolated and highly purified by using Chromatofocusing and affinity chromatography. The amount of 3 α-hydroxysteroid UDP-glucuronosyltransferase in the liver of Wistar rats exhibiting low rates for androsterone glucuronidation is about 10% or less than that found in hepatic microsomal fractions obtained from Wistar rats having high rates for androsterone glucuronidation. The apparent Km for androsterone with purified 3 α-hydroxysteroid UDP-glucuronosyltransferase from Wistar rats with high glucuronidation activity (6 microM) was not different from that observed for the enzyme purified from Sprague-Dawley animals, whereas that for the enzyme purified from Wistar rats with low glucuronidation activity was substantially higher (120 microM). Despite the differences in apparent Km values for androsterone, the apparent Km for UDP-glucuronic acid (0.3 mM) was not different in the different populations of rats.

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Nagai, J., S. Watanabe, J. Kobayashi, and S. Sugawara. "Intra uterine survival and growth of Wistar and Wistar × Brown Norway rat embryos." Theriogenology 23, no. 2 (February 1985): 361–68. http://dx.doi.org/10.1016/0093-691x(85)90038-x.

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Efanov, Alexander M., Ioulia B. Appelskog, Samy M. Abdel-Halim, Akhtar Khan, Robert Bränström, Olof Larsson, Claes-Göran Östenson, et al. "Insulinotropic activity of the imidazoline derivative RX871024 in the diabetic GK rat." American Journal of Physiology-Endocrinology and Metabolism 282, no. 1 (January 1, 2002): E117—E124. http://dx.doi.org/10.1152/ajpendo.000031.2001.

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The insulinotropic activity of the imidazoline derivative RX871024 was compared in pancreatic islets from nondiabetic Wistar rats and spontaneously diabetic Goto-Kakizaki (GK) rats. RX871024 significantly stimulated insulin secretion in islets from both animal groups. The insulinotropic activity of RX871024 was higher than that of the sulfonylurea glibenclamide. This difference was more pronounced in islets from GK rats compared with Wistar rat islets. More importantly, RX871024 substantially improved glucose sensitivity in diabetic β-cells, whereas glibenclamide stimulated insulin secretion about twofold over a broad range of glucose concentrations in nondiabetic and diabetic rats. RX871024 induced a faster increase in cytosolic free Ca2+ concentration and faster inhibition of ATP-dependent K+ channel activity in GK rat islets compared with Wistar rat islets. RX871024 also induced a more pronounced increase in diacylglycerol concentration in GK rat islets. These data support the idea that imidazoline compounds can form the basis for the development of novel drugs for treatment of type 2 diabetes, which can restore glucose sensitivity in diabetic β-cells.

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Taofik, Ahmad, and Depison Depison. "Pengaruh Nisbah Kelamin Perkawinan Terhadap Litter Size dan Jumlah Anak Perkandang Perkawinan Tikus Putih Wistar." Jurnal Ilmiah Ilmu-Ilmu Peternakan 11, no. 3 (August 1, 2008): 118–24. http://dx.doi.org/10.22437/jiiip.v11i3.743.

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This research was conducted to know the influence of sex ratio parental on pregnant females, litter size and output per breed of white wistar rat. Experiment started on January 1st – March 10th, 2008. This experiment sample was taken from PAU Institute Teknology Bandung and it done at Garden of Botany of Faculty of Mathematics and Natural Sciences of University of Education Indonesia. As many 128 white wistar rat consisted of 112 females mating old (virgin) with body weight 162-235 gram and 16 males mating old with body weight 275-325 gram placed randomly into cage 60 cm x 40 cm x 25 cm. This research was used experiment methode with Completely Randomized Design (CRD). The treatment, were R1 = 1 : 4 (1 male and 4 females), R2 = 1 : 6 (1 male and 6 females), R3 = 1 : 8 (1 male and 8 females) dan R4= 1 : 10 (1 male and 10 females). The placement was according to treatment group and each treatment repeated four times. Males and females oestrus kept together until five days, account the pregnant females, litter size, and output per breed of white wistar rat. Analysis of Variance was used to analyse the data. The result of this research indicated that sex ratio not significantly (P > 0,05) on pregnant females, litter size and output per breed of white wistar rat.

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Louis, W. J., and L. G. Howes. "Genealogy of the Spontaneously Hypertensive Rat and Wistar-Kyoto Rat Strains." Journal of Cardiovascular Pharmacology 16 (1990): S1—S5. http://dx.doi.org/10.1097/00005344-199000167-00002.

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Louis, W. J., and L. G. Howes. "Genealogy of the Spontaneously Hypertensive Rat and Wistar-Kyoto Rat Strains." Journal of Cardiovascular Pharmacology 16 (1990): S1—S5. http://dx.doi.org/10.1097/00005344-199006167-00002.

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33

Fukui, *Kiyoshiro, Takuya Kawada, and Kenichi Fukumoto. "HYPERACTIVITY IN BASOLATERAL AMYGDALA MAY CONTRIBUTE TO THE NEURAL CIRCUIT PATHOLOGY IN TREATMENT-RESISTANT DEPRESSION MODEL." International Journal of Neuropsychopharmacology 28, Supplement_1 (February 2025): i205. https://doi.org/10.1093/ijnp/pyae059.356.

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Abstract Background Major depressive disorder (MDD) is one of the diseases that makes social life extremely difficult and has a heavy medical economic burden. Currently antidepressant agents such as selective serotonin reuptake inhibitors (SSRIs) are inefficient in improving depressive symptoms in about 30 % of patients, and there is a need for drugs with new mechanism of action. Aims & Objective Wistar Kyoto (WKY) rat strain has been suggested as a useful tool for investigating the pathophysiology of treatment-resistant depression because long-term administration of SSRIs can’ t improve depressive-like symptoms in WKY rat. However, the neural characteristics that WKY rat shows the phenotypes like patients who don’ t respond to antidepressants is unknown. The basolateral amygdala (BLA) plays critical roles that expressing moods and emotions including anxiety and depressive symptoms. Meta-analyses of 367 task-related fMRI experiments in mood disorders, posttraumatic stress disorder, and anxiety disorders have demonstrated that hyperactivity in the amygdala is associated with negative valence systems. Therefore, the hyperactivity in BLA could be a key pathophysiology in MDD. In this study, we evaluated the predictive validity and neural excitability of excitatory projection neurons (PNs) in BLA of WKY rat as a model of treatment-resistant depression. Method & Result First, we confirmed the predictive validity for SSRIs by performing the Forced Swim Test (FST). Consistent with previous studies, the immobility time of WKY rat was significantly longer than Wistar rat, which has same genetic background as WKY rat. Multiple administration of fluoxetine didn’ t reduce the immobility time of WKY rat. Next, we performed whole-cell patch clamp recording to investigate neural activity of PNs in BLA. The number of spontaneous action potentials was significantly increased in WKY rat compared with Wistar rat under the condition of voltage-clamp at -70 mV. Moreover, WKY rat exhibited a higher number of firings than Wistar rat in response to steady increasing stimulation. In the contrast, no significant differences were found in the frequency and amplitude of spontaneous inhibitory postsynaptic currents (sIPSC) between WKY rat and Wistar rat. Discussion & Conclusion Collectively, these results suggest that BLA PNs of WKY rat are hyperactive by increasing neural excitability but not inhibitory synaptic mechanism. Positive allosteric modulators for synaptic and extrasynaptic GABAA receptors have been found to elicit both immediate and long-lasting antidepressant effects in patients with Major Depressive Disorder (MDD) or Postpartum Depression (PPD). Recent findings suggest that activation of the extrasynaptic GABAARs may play a crucial role in the antidepressant effects. Hence, we now verify the effect of 4,5,6,7-tetrahydroisoxazolo (5,4-c) pyridin-3 (-ol) (THIP), a ligand with the activity of extrasynaptic GABAARs on the neural excitability of PNs in BLA to clarify the possible neural mechanisms underlying the antidepressant effects due to the activity of extrasynaptic GABAARs. We would like to show our new behavioral and electrophysiological data.

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Jackson, CW, NK Hutson, SA Steward, and PE Stenberg. "A unique talin antigenic determinant and anomalous megakaryocyte talin distribution associated with abnormal platelet formation in the Wistar Furth rat." Blood 79, no. 7 (April 1, 1992): 1729–37. http://dx.doi.org/10.1182/blood.v79.7.1729.1729.

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Abstract Rats of the Wistar Furth (WF) strain have hereditary macrothrombocytopenia with decreased platelet alpha-granule proteins. The autosomal recessive pattern of inheritance of the large mean platelet volume (MPV) phenotype and platelet alpha-granule protein deficiencies suggest that a component common to both formation of platelet alpha-granules and subdivision of megakaryocyte cytoplasm into platelets is quantitatively or qualitatively abnormal in WF megakaryocytes and platelets. We examined WF platelets for such an abnormality using electrophoretic and immunologic analyses. Rabbit antiserum prepared against WF rat platelets and absorbed with Wistar rat platelets recognized a major 235-Kd band, and minor bands of WF rat platelets ranging from 200 to 130 Kd, not present in immunoblots of Wistar, Sprague-Dawley, or Long-Evans rat platelets. The minor bands were labeled with affinity-isolated antibody to the 235-Kd band, indicating that all bands contained the same unique antigenic site. The 235-Kd antigen had the same mobility as rat platelet talin identified with a platelet antitalin antibody. Activation of calcium-dependent proteases during Triton X-100 extraction caused conversion of the 235- Kd antigen into a major fragment of 200 Kd and minor fragments ranging to 115 Kd, identical in mobility to fragments of rat platelet talin produced in the same samples. The absorbed anti-WF platelet antiserum also detected a 235-Kd antigen in WF lung, kidney, and small intestine by immunoblotting. Finally, the 235-Kd antigen unique to WF rats was immunoprecipitated from Triton X-100 supernatants of WF platelets with an antitalin monoclonal antibody (MoAb). These data indicate that the unique antigenic site is on WF talin. Examination of talin distribution in Wistar megakaryocytes showed localization beneath the plasma membrane, on the cytosolic face of demarcation membranes, associated with alpha-granule membranes, and diffusely throughout the cytoplasm. Although WF megakaryocytes showed the same general distribution pattern, some differences were apparent. In contrast to membrane systems of the Wistar rat, the large membrane complexes in WF megakaryocytes contained little or no talin. In addition, approximately half of WF megakaryocytes showed an increased peripheral localization of talin, often associated with membrane blebs, with decreased talin in the cytoplasmic interior. The association of the unique talin antigenic determinant and anomalous megakaryocyte talin distribution with abnormal platelet formation in WF rats suggests that talin is abnormal in this rat strain and that talin plays an important role in subdivision of megakaryocyte cytoplasm into platelets.

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Jackson, CW, NK Hutson, SA Steward, and PE Stenberg. "A unique talin antigenic determinant and anomalous megakaryocyte talin distribution associated with abnormal platelet formation in the Wistar Furth rat." Blood 79, no. 7 (April 1, 1992): 1729–37. http://dx.doi.org/10.1182/blood.v79.7.1729.bloodjournal7971729.

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Rats of the Wistar Furth (WF) strain have hereditary macrothrombocytopenia with decreased platelet alpha-granule proteins. The autosomal recessive pattern of inheritance of the large mean platelet volume (MPV) phenotype and platelet alpha-granule protein deficiencies suggest that a component common to both formation of platelet alpha-granules and subdivision of megakaryocyte cytoplasm into platelets is quantitatively or qualitatively abnormal in WF megakaryocytes and platelets. We examined WF platelets for such an abnormality using electrophoretic and immunologic analyses. Rabbit antiserum prepared against WF rat platelets and absorbed with Wistar rat platelets recognized a major 235-Kd band, and minor bands of WF rat platelets ranging from 200 to 130 Kd, not present in immunoblots of Wistar, Sprague-Dawley, or Long-Evans rat platelets. The minor bands were labeled with affinity-isolated antibody to the 235-Kd band, indicating that all bands contained the same unique antigenic site. The 235-Kd antigen had the same mobility as rat platelet talin identified with a platelet antitalin antibody. Activation of calcium-dependent proteases during Triton X-100 extraction caused conversion of the 235- Kd antigen into a major fragment of 200 Kd and minor fragments ranging to 115 Kd, identical in mobility to fragments of rat platelet talin produced in the same samples. The absorbed anti-WF platelet antiserum also detected a 235-Kd antigen in WF lung, kidney, and small intestine by immunoblotting. Finally, the 235-Kd antigen unique to WF rats was immunoprecipitated from Triton X-100 supernatants of WF platelets with an antitalin monoclonal antibody (MoAb). These data indicate that the unique antigenic site is on WF talin. Examination of talin distribution in Wistar megakaryocytes showed localization beneath the plasma membrane, on the cytosolic face of demarcation membranes, associated with alpha-granule membranes, and diffusely throughout the cytoplasm. Although WF megakaryocytes showed the same general distribution pattern, some differences were apparent. In contrast to membrane systems of the Wistar rat, the large membrane complexes in WF megakaryocytes contained little or no talin. In addition, approximately half of WF megakaryocytes showed an increased peripheral localization of talin, often associated with membrane blebs, with decreased talin in the cytoplasmic interior. The association of the unique talin antigenic determinant and anomalous megakaryocyte talin distribution with abnormal platelet formation in WF rats suggests that talin is abnormal in this rat strain and that talin plays an important role in subdivision of megakaryocyte cytoplasm into platelets.

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Weber, Klaus. "Differences in Types and Incidence of Neoplasms in Wistar Han and Sprague-Dawley Rats." Toxicologic Pathology 45, no. 1 (January 2017): 64–75. http://dx.doi.org/10.1177/0192623316672075.

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A substantial quantity of data on Sprague-Dawley (SD) and Hannover Wistar rats strains have been published concerning their source, diet, and housing conditions, as well as the incidences of nonneoplastic lesions and neoplasms observed in different laboratories. Differences between the commonly used rat strains provided by different breeders (i.e., CD (SD) vs. Harlan Sprague-Dawley strain or Crl: WI(Han) vs. Wistar Hannover (Han)–derived strain, continued breeding by RCC Ltd., Switzerland, thereafter continued breeding by Harlan) may include, but are not limited to, body weight, incidence, and onset of major nonneoplastic lesions and neoplasms, and these can impact the development of a nonclinical safety program. Fisher 344 (F344) and SD rat strains generally have the highest tumor incidences, exceeding that in Wistar rats. Certain tumors are more commonly observed in one strain, and for some, the difference in incidence may be so significant that the tumor may even be considered characteristic for a specific strain (e.g., thymoma in Wistar and amphophilic renal adenoma in SD).

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Arini, Pedro David, Sergio Liberczuk, Javier Gustavo Mendieta, Martín Santa María, and Guillermo Claudio Bertrán. "Electrocardiogram Delineation in a Wistar Rat Experimental Model." Computational and Mathematical Methods in Medicine 2018 (2018): 1–10. http://dx.doi.org/10.1155/2018/2185378.

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Background and Objectives. The extensive use of electrocardiogram (ECG) recordings during experimental protocols using small rodents requires an automatic delineation technique in the ECG with high performance. It has been shown that the wavelet transform (WT) based ECG delineator is a suitable tool to delineate electrocardiographic waveforms. The aim of this work is to implement and evaluate the ECG waves delineation in Wistar rats applying WT. We also describe the ECG signal of the Wistar rats giving the characteristics of its spectrum among other useful information. Methods. We evaluated a delineator based on WT in a Wistar rat electrocardiograms database which was annotated manually by experienced observers. Results. The delineation showed an “overall performance” such as sensitivity and a positive predictive value of 99.2% and 83.9% for P-wave, 100% and 99.9% for QRS complex, and 100% and 99.8% for T-wave, respectively. We also compared temporal analysis based ECG delineator with the WT based ECG delineator in RR interval, QRS duration, QT interval, and T-wave peak-to-end duration. The results showed that WT outperforms the temporal delineation technique in all parameters analyzed. Conclusions. Finally, we propose a WT based ECG delineator as a methodology to implement in a wide diversity of experimental ECG analyses using Wistar rats.

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Soulage, Christophe, Bader Zarrouki, Anisio Francesco Soares, Michel Lagarde, and Alain Geloen. "Lou/C Obesity-resistant Rat Exhibits Hyperactivity, Hypermetabolism, Alterations in White Adipose Tissue Cellularity, and Lipid Tissue Profiles." Endocrinology 149, no. 2 (November 15, 2007): 615–25. http://dx.doi.org/10.1210/en.2007-0317.

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Lou/C obesity-resistant rat constitutes an original model to understand the phenomena of overweight and obesity. The aim of the present study was to identify metabolic causes for the outstanding leanness of Lou/C rat. To this end, the metabolic profiles (food intake, energy expenditure, and physical activity) and the cellular characteristics of white adipose tissue (lipogenesis, lipolysis, cellularity, and lipid composition) in 30-wk-old Lou/C rats were compared with age-matched Wistar rats. Lou/C rats exhibited a lower body weight (−45%), reduced adiposity (−80%), increased locomotor activity (+95%), and higher energy expenditure (+11%) than Wistar rats. Epididymal adipose tissue of Lou/C rat was twice lower than that of Wistar rat due to both a reduction in both adipocyte size (−25%) and number (three times). Basal lipolysis and sensitivity to noradrenaline were similar; however, the responsiveness to noradrenaline was lower in adipocytes from Lou/C compared with that from Wistar rats. Lipidomic analysis of plasma, adipose tissue, and liver revealed profound differences in lipid composition between the two strains. Of note, the desaturation indexes (ratio C16:1/C16:0 and C18:1/C18:0) were lower in Lou/C, indicating a blunted activity of δ-9-desaturase such as stearoyl-coenzyme A-desaturase-1. Increased physical activity, increased energy expenditure, and white adipose tissue cellularity are in good agreement with previous observations suggesting that a higher sympathetic tone in Lou/C could contribute to its lifelong leanness.

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Maanari, Chaleb P., Edi Suryanto, and Julius Pontoh. "Aktivitas Penangkal Radikal Hidroksil Fraksi Flavonoid dari Limbah Tongkol Jagung pada Tikus Wistar." Jurnal MIPA 3, no. 2 (August 13, 2014): 134. http://dx.doi.org/10.35799/jm.3.2.2014.5990.

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Penelitian telah dilakukan untuk menentukan kemampuan menangkal radikal hidroksil pada homogenat hati, jantung dan otak tikus wistar dari fraksi flavonoid limbah tongkol jagung. Penelitian dimulai dengan mengekstraksi serbuk tongkol jagung yang sudah dikering anginkan menggunakan cara refluks selama dua jam dengan pelarut etanol 80%. Ekstrak kemudian dipartisi berturut-turut menggunakan petroleum eter, etil asetat, n-butanol dan air, selanjutnya ditentukan kandungan total flavonoid serta aktivitas penangkal radikal hidroksil pada homogenat jaringan hati, jantung dan otak tikus wistar. Hasilnya menunjukkan ekstrak etil asetat yang memiliki kandungan total flavonoid yang paling tinggi yaitu 41,926 mg/kg ekstrak, serta kemampuan menangkal radikal hidroksil pada homogenat jaringan hati, jantung dan otak tikus wistar sebesar 90,964%; 86,875% dan 68,235%.This research has been conducted to determine radical hydroxyl scavenging ability on a wistar rat tissue homogenate of liver, heart and brain from corn cob waste extract. Research started with with extracting the powder of corn cob which had been air-dried using reflux method during two hours with 80% ethanol solvent. Then the extract successively partitioned with petroleum ether, ethyl acetate, n-buthanol and water, next determined the flavonoid total content with activities of hydroxyl radical on a wistar rat tissue homogenate. The result showed ethyl acetate extract which has good result for flavonoid total content that is 41,926 mg/kg extract, with ability of free radical scavenger on a rat tissue homogenate of liver, heart and brain as much as 90,964%; 86,875% and 68,235%.

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Ridwan, Rini Devijanti, Wisnu Setyari Juliastuti, and R. Darmawan Setijanto. "Effect of electrolyzed reduced water on Wistar rats with chronic periodontitis on malondialdehyde levels." Dental Journal (Majalah Kedokteran Gigi) 50, no. 1 (March 31, 2017): 10. http://dx.doi.org/10.20473/j.djmkg.v50.i1.p10-13.

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Background: Periodontal disease is a progressive destructive change that causes loss of bone and periodontal ligaments around the teeth that can eventually lead to its loss. The main bacteria in chronic periodontitis is Porphyromonas gingivalis. Aggregatibacter actinomycetemcomitans, a pathogen associated with aggressive periodontitis, initiates a proinflammatory response that causes tissue destruction of periodontal, alveolar bone resorption and subsequent tooth loss. Electrolyzed reduced water (ERW) is an alkaline water, ERW not only has a high pH and low oxidation reduction potential (ORP), but also contains several magnesium ions. Magnesium ions proven effective for the prevention of various diseases. Purpose: To analyze the level of malondialdehyde (MDA) in Wistar rats with cases of chronic and aggressive periodontitis that consumed ERW. Method: Wistar rats were divided into four groups, each group with 10 rats. The first and second group were Wistar rat with chronic periodontitis and consume drinking water and ERW. The third and fourth group were Wistar rat with aggressive periodontitis and consume drinking water and ERW. This experiment is done by calculating the levels of MDA. The calculation of the levels of MDA is done with spectrophotometric assay for MDA. Result: The results of this experiment show that the level of MDA in serum in group that consume ERW had decreased significantly different with thegroup that consume drinking water with the statistical test. Conclusion: It can be concluded that ERW can decrease the MDA level in Wistar rat with chronic and aggressive periodontitis case.

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Odo, MO, PA Okorie, F. Azi, and VN Nwobasi. "Effect of Wheat/Cassava Composite Bread on Liver Architecture of Wistar Rat." Journal of Pure and Applied Microbiology 12, no. 1 (March 30, 2018): 151–59. http://dx.doi.org/10.22207/jpam.12.1.19.

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Hamutoğlu, Rasim, and Serpil Ünver Saraydin. "HISTOLOGICAL EVALUATION OF THE RAT CRANIAL REGION." Selçuk Sağlık Dergisi 6, no. 1 (February 28, 2025): 77–96. https://doi.org/10.70813/ssd.1512662.

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Purpose: This study was to ascertain the histological evaluation of some organs in the cranial region of newborn Wistar albino rats, such as the tongue, palate, and Steno's gland in the nasal cavity, compared to adult rats. Methods: 5 healthy female newborn Wistar albino rats were used in the study. Tissues obtained from rats were fixed in 10% formalin for 2 days. The histological features of the tongue, palate and nasal cavity were revealed after Hematoxylin&eosin and Mallory’s Azan stainings. Results: While there was fatty tissue in the submucosa layer in the ventral section of the soft palate, mucus glands were observed in the dorsal section. The main papillae were filiform and fungiform, and scattered foliate and circumvallate papillae were also present. The lateral nasal glands (Steno’s glands) were well developed. In general, there were four ethmoturbinates in coronal and sagittal sections in the posterosuperior part of the unilateral sinus in adults, whereas there were two in neonatal rats. Unusually, a septal window was visible immediately rostral to the nasopharynx in the mouse, however, no septal window was observed in newborn rats. Conslusion: This study reports basic research features on the anatomy of the oral and nasal cavity of the newborn Wistar albino rat. Our data may shed light on other studies aimed at fully investigating the structure of these organs, which may be useful in subsequent experimental and morphological studies on newborn Wistar albino rats.

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Kozhevnikova, O. S., and N. G. Kolosova. "Analysis of microRNA expression in rat retina at the early stages of retinopathy development." Genes & Cells 18, no. 4 (December 15, 2023): 498–501. http://dx.doi.org/10.17816/gc623463.

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Age-related macular degeneration (AMD), a neurodegenerative eye disease, is the primary cause of blindness globally, especially affecting the elderly population. MicroRNAs, a type of single-stranded, non-coding RNAs of about 22 nucleotides in length, primarily regulate gene expression negatively. Alterations in the miRNA profile during AMD’s development could potentially aid in early detection and progression monitoring of the disease. However, no data are available on the microRNA expression patterns in the retina during the early stages of AMD. Investigating the molecular mechanisms that underlie age-related retinal degeneration at an early stage, prior to the manifestation of any symptoms, could provide insights into the molecular events that initiate the irreversible stage. In the case of OXYS rats with accelerated aging, dystrophic changes in the RPE, neuroretinal thinning, and impaired choroidal microcirculation — the primary indicators of the dry form of AMD — spontaneously occur by the age of 3 months [1]. A comparative analysis was conducted in this study on microRNA expression in the retinas of OXYS rats at two different stages of retinopathy — 20 days (preclinical) and 3 months (manifestation) — as well as in control Wistar rats. Small RNA-seq sequencing was performed on the DNBSEQ platform. According to differential expression analysis (DESeq2, q-value 0.05), at 20 days of age, 2 microRNAs exhibited altered expression in OXYS rats compared to Wistar rats. Similarly, at 3 months of age, the expression of 16 microRNAs in OXYS rats was altered compared to Wistars, with 7 miRNAs showing increased levels and 9 miRNAs showing decreased levels. Analysis of age-related changes in miRNA expression demonstrated that in the OXYS rat retina between 20 days and 3 months, the levels of 134 miRNAs altered: 59 miRNAs increased, and 75 miRNAs decreased. Over the course of 20 days to 3 months, 94 miRNAs underwent alterations in the retinas of Wistar rats. Among these, the level of 48 miRNAs increased while the level of 46 miRNAs decreased. The RNAhybrid, miRanda, and TargetScan programs were used to search for target genes of differentially presented miRNAs among different groups. Following this, gene ontologies were studied. In comparison to Wistar rats, “adhesion contacts” and “synaptic vesicle cycle” categories are noticeably populated with target genes of miRNA DE at 20 days in OXYS rats. MiRNA target genes with lower levels at 3 months in OXYS rats compared to Wistar rats are significantly enriched in categories such as vascular branching, extracellular matrix organization, adhesion, and protein deubiquitination. Target genes with increased miRNA levels at 3 months in OXYS rats, compared to Wistar rats, are significantly represented in various signaling pathways, such as mTOR, MAPK, VEGF, and thyroid hormone. Target genes, which serve as common targets for at least 10 microRNAs (miRNAs), were chosen for analysis of the targetome subject to regulation by miRNA molecules exhibiting modified expression. Targetomes containing 400 to 600 genes were obtained for miRNAs that increased or decreased levels in both OXYS and Wistar rats between 20 days and 3 months. Notably, these targetomes were enriched in categories such as axonogenesis, retinal layer formation, visual learning, focal adhesion, and endocytosis. Profiling of 84 miRNAs of the retina of OXYS and Wistar rats at the age of 20 days and 3 months was carried out using PCR panels (Qiagen) to verify the results of small RNA-seq. Our analysis revealed highly convergent results between sequencing and PCR panels. Thus, 16 miRNAs showing altered expression in OXYS retina may serve as potential biomarkers and new targets for studying AMD pathogenesis.

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Hu, Ding-Yu, Qin Li, Bo Li, Rong-Ji Dai, Li-Na Geng, and Yu-Lin Deng. "Normobaric hypoxia-induced brain damage in wistar rat." Journal of Biomedical Science and Engineering 02, no. 08 (2009): 632–36. http://dx.doi.org/10.4236/jbise.2009.28092.

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Mori, M., T. Kawajiri, M. Sayama, Y. Taniuchi, T. Miyahara, and H. Kozuka. "Metabolism of 2,6-Dinitrotoluene in Male Wistar Rat." Xenobiotica 19, no. 7 (January 1989): 731–41. http://dx.doi.org/10.3109/00498258909042311.

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Xie, Zhi, Junming Zhang, Liyan Xi, Xiqing Li, Liangchun Wang, Changming Lu, and Jiufeng Sun. "A chronic chromoblastomycosis model byFonsecaea monophorain Wistar rat." Medical Mycology 48, no. 1 (February 2010): 201–6. http://dx.doi.org/10.3109/13693780902785320.

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Ai, Jing. "Development of Wistar rat model of insulin resistance." World Journal of Gastroenterology 11, no. 24 (2005): 3675. http://dx.doi.org/10.3748/wjg.v11.i24.3675.

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Blaszczyk, Wanda M., Larissa Arning, Klaus-Peter Hoffmann, and Joerg T. Epplen. "ATyrosinasemissense mutation causes albinism in the Wistar rat." Pigment Cell Research 18, no. 2 (April 2005): 144–45. http://dx.doi.org/10.1111/j.1600-0749.2005.00227.x.

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Reindel, J., W. Bobrowski, A. Gough, and J. Anderson. "Malignant Intracranial Teratoma in a Juvenile Wistar Rat." Veterinary Pathology 33, no. 4 (July 1996): 462–65. http://dx.doi.org/10.1177/030098589603300421.

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An intracranial malignant teratoma was identified in a 91-day-old male Wistar rat manifesting central nervous system-related clinical signs. This tumor occupied the right midbrain and portions of the right caudal cerebrum and cranioventral cerebellum. Microscopically, the tumor contained intermingled cartilage, bone (with medullary hematopoietic tissue), fibrous connective tissue, skeletal muscle, fat, pseudostratified ciliated epithelium, stratified squamous epithelium, serous and mucoserous glands, and neural tissue with ependymal and choroid plexus epithelia. Poorly differentiated cells with primitive cartilaginous matrix were present throughout the lining of lateral ventricles, in the aqueduct of Sylvius, and in meninges overlying normal cerebellar tissue indicating tumor metastasis occurred via cerebrospinal fluid. This neoplasm was not identified in extracranial sites and hence was considered a primary intracranial malignant teratoma with metastases via cerebrospinal fluid.

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Heinrichs, Martin, and Heinrich Ernst. "Spontaneous malignant craniopharyngioma in an aged Wistar rat." Journal of Toxicologic Pathology 29, no. 3 (2016): 195–99. http://dx.doi.org/10.1293/tox.2016-0004.

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Journal articles on the topic 'Rat (wistar'

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