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Journal articles on the topic 'Re-Used fragments'

Author: Grafiati

Published: 4 June 2021

Last updated: 12 February 2022

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1

Dévai, Kata. "Re-Used Glass Fragments from Intercisa." Dissertationes Archaeologicae 3, no. 7 (2020): 187–204. http://dx.doi.org/10.17204/dissarch.2019.187.

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Re-worked glass objects are known all over the Roman Empire, but detailed analysis of various types of re-working sherds have not yet been undertaken. In the following study, I give some examples of some interesting uses from Intercisa. The over thirty re-used glass vessel fragments from Intercisa are noteworthy since they come from the find material of a single vicus. It seems likely that the repurposing of broken glass vessels was a more common practice than the currently available publications would suggest. In all likelihood, it made good sense to re-usethe fragments of broken glass vessel

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Chen, Zhikun, Shuqiang Yang, Yunfei Shang, et al. "Fragment Re-Allocation Strategy Based on Hypergraph for NoSQL Database Systems." International Journal of Grid and High Performance Computing 8, no. 3 (2016): 1–23. http://dx.doi.org/10.4018/ijghpc.2016070101.

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NoSQL database is famed for the characteristics of high scalability, high availability, and high fault-tolerance. It is used to manage data for a lot of applications. The computing model has been transferred to “computing close to data”. Therefore, the location of fragment directly affects system's performance. Every site's load dynamical changes because of the increasing data and the ever-changing operation pattern. So system has to re-allocate fragment to improve system's performance. The general fragment re-allocation strategies of NoSQL database scatter the related fragments as possible to

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Chowdhury, SMZH, AR Omar, A. Ideris, H. Bejo, and AA Jamaluddin. "Restriction endonuclease analysis of the genomes of different isolates of chicken anemia virus amplified by polymerase chain reaction." SAARC Journal of Agriculture 15, no. 2 (2018): 1–18. http://dx.doi.org/10.3329/sja.v15i2.35155.

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Four DNA fragments (fragments A, B, C and D) covering the whole genome of chicken anemia virus (CAV) were amplified enzymatically by polymerase chain reaction (PCR) using four pairs of oligonucleotide primers. The DNA fragments were amplified from each of nine CAV isolates including eight Malaysian isolates and one European isolate (Cux-1). For all nine CAV isolates, fragment A (1518 bp) was digested with one restriction enzyme, Eco130I (StyI); fragment B (926 bp) with three enzymes, Eco130I (StyI), HpaII and MboI separately; fragment C (675 bp) with also three enzymes, BsuRI (HaeIII), HinfI,

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4

Fang, Jinggui, Pachanoor S. Devanand, and ChihCheng T. Chao. "(270) Single-nucleotide-polymorphism Isolation in Fruiting-mei (Prunus mume Sieb. et Zucc.) from AFLP Fragments." HortScience 40, no. 4 (2005): 1020B—1020. http://dx.doi.org/10.21273/hortsci.40.4.1020b.

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Single-nucleotide-polymorphism (SNP) is the most abundant genetic variation among individuals within a species. SNPs can be used as markers for gene discovery and for assessment of biodiversity. We established a practical strategy for discovering candidate SNPs in fruiting-mei (Prunusmume Sieb. et Zucc.), a non-model tree fruit, from amplified-fragment-length-polymorphism (AFLP) fragments. Eighty-one of the 150 chosen bands from 10 cultivars of fruiting-mei were successfully re-amplified and 67 of these re-amplified PCR products yielded 13 groups of reliable sequences. The sequencing results f

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Trinchera, M., R. Ghidoni, S. Sonnino, and G. Tettamanti. "Recycling of glucosylceramide and sphingosine for the biosynthesis of gangliosides and sphingomyelin in rat liver." Biochemical Journal 270, no. 3 (1990): 815–20. http://dx.doi.org/10.1042/bj2700815.

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It was previously shown that sphingomyelin and gangliosides can be biosynthesized starting from sphingosine or sphingosine-containing fragments which originated in the course of GM1 ganglioside catabolism. In the present paper we investigated which fragments were specifically re-used for sphingomyelin and ganglioside biosynthesis in rat liver. At 30 h after intravenous injection of GM1 labelled at the level of the fatty acid ([stearoyl-14C]GM1) or of the sphingosine ([Sph-3H]) moiety, it was observed that radioactive sphingomyelin was formed almost exclusively after the sphingosine-labelled-GM

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Pascuttini-Juraga, Vesna, and Ivana Peškan. "Spolia - Hidden Codes of the Past. Re-used Fragments of Carved Stone Elements in Varaždin County." Hortus Artium Medievalium 17 (January 2011): 175–84. http://dx.doi.org/10.1484/j.ham.1.102280.

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Poeplau, Christopher, Cora Vos, and Axel Don. "Soil organic carbon stocks are systematically overestimated by misuse of the parameters bulk density and rock fragment content." SOIL 3, no. 1 (2017): 61–66. http://dx.doi.org/10.5194/soil-3-61-2017.

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Abstract. Estimation of soil organic carbon (SOC) stocks requires estimates of the carbon content, bulk density, rock fragment content and depth of a respective soil layer. However, different application of these parameters could introduce a considerable bias. Here, we explain why three out of four frequently applied methods overestimate SOC stocks. In soils rich in rock fragments (> 30 vol. %), SOC stocks could be overestimated by more than 100 %, as revealed by using German Agricultural Soil Inventory data. Due to relatively low rock fragments content, the mean systematic overestimation f

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Islam, Mohammad Aminul, Tasnim Wakia, Md Shamsul Alam, Md Mujibur Rahman Howlader, and Shamima Afroz. "Management of a Subgingivally Fractured Central Incisor by Re-attachment Using a Fiber Post." Update Dental College Journal 3, no. 1 (2014): 37–40. http://dx.doi.org/10.3329/updcj.v3i1.17983.

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Reattachment of a fractured fragment to the remaining tooth is challenging but one of the best treatment protocol in regards to aesthetics, function as well as patients acceptance. This case report presents a 20 years old male with an oblique complicated crown fracture of maxillary left central incisor tooth. The procedure used to repair the fracture regarding this case included flap surgery with endodontic treatment. The root canal was filled with a root canal sealer and gutta-percha. After root canal obturation, fragments were reattached with duel cure composite resin using a glass fibre pos

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9

Manca, F., G. Li Pira, D. Fenoglio, et al. "Recognition of human T-leukemia virus (HTLV-1) envelope by human CD4+ T- cell lines from HTLV-1 seronegative individuals: specificity and clonal heterogeneity." Blood 85, no. 6 (1995): 1547–54. http://dx.doi.org/10.1182/blood.v85.6.1547.bloodjournal8561547.

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Because T-helper cells are critical for immune responses in retroviral infections, CD4+ T-cell lines specific for the human T-leukemia virus type 1 (HTLV-1) envelope have been generated from peripheral T lymphocytes of nonimmune donors to study their naive repertoire. Recombinant fragments (RE1, amino acids [aa] 26–200; RE3, aa 165–307; RE5, aa 308–401; and RE6, aa 165–401) of HTLV-1 envelope, whole envelope glycoprotein, and synthetic peptides were used to induce T- cell lines. CD4+ T-cell lines specific for one or more fragments were obtained from seven of eight individuals tested. T-cell li

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10

Siegert, Christine. "Altpapierverwertung im 18. Jahrhundert: Fragmente in den Aufführungsmaterialen des Esterházy’schen Opernbetriebs." Studia Musicologica 51, no. 3-4 (2010): 277–301. http://dx.doi.org/10.1556/smus.51.2010.3-4.4.

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In the opera materials of the Esterházy Court in the Széchényi National Library in Budapest, that have survived from the time when Haydn directed the opera at Eszterháza, there are several inserted papers that had been used before the sheet of paper was re-used for the adaptation of the given opera. The fragmentary notations vary in content and extent. Two groups are of special interest for Haydn scholars: fragments that can be related to Haydn’s operas and fragments written by Haydn. The latter are listed in the appendix in a catalogue that might be expanded in the future.

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11

Tiep, N. V., L. H. Khiem, A. S. Sohatsky, et al. "TEM Study of ODS Alloy Doped with Helium Ions and Re-irradiated with Swift Xe Ions." Communications in Physics 29, no. 3SI (2019): 377. http://dx.doi.org/10.15625/0868-3166/29/3si/14286.

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Oxide dispersion strengthened (ODS) alloys are considered as candidates for fuel assembly claddings, since nano-oxides, among other things, contribute to the gas swelling suppression. However, it is still unknown that how oxides will behave in this capacity under the exposure to fission fragments. Transmission electron microscopy has been applied to study helium porosity in a ferritic EP450ODS alloy doped with helium by the ion implantation and re-irradiated with high energy Xe ions, which is used for simulating fission fragments at room temperature followed by annealing. It has been shown tha

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Nuroniah, H. S., O. Gailing, and R. Finkeldey. "Development of SCAR Markers for Species Identification in the Genus Shorea (Dipterocarpaceae)." Silvae Genetica 59, no. 1-6 (2010): 249–57. http://dx.doi.org/10.1515/sg-2010-0035.

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AbstractThe development of molecular markers unambiguously distinguishing groups at different taxonomic levels has numerous forensic applications. The identification of tropical timber is of particular interest in this context. We describe the development of SCAR (Sequence Characterized Amplified Region) markers for forensic applications taking the example of two closely related species of the tropical tree family Shorea (Dipterocarpaceae). Two AFLP (Amplified Fragment Length Polymorphism) fragments have been described earlier showing strong differentiation between S. leprosula and S. parvifol

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13

Bertagnolli, Davide. "De Spiegel historiael in Italië: onbekende fragmenten te Valenza." Tijdschrift voor Nederlandse Taal- en Letterkunde 135, no. 3 (2019): 260–95. http://dx.doi.org/10.5117/tntl2019.3.005.bert.

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Abstract The public library of Valenza, in the Italian region of Piedmont, holds two parchment fragments of Jacob van Maerlant’s Spiegel historiael which are used to protect other documents. The present paper is the first study dedicated to them. The leaves are first described separately, highlighting their shape as well as the text which has been handed down to us. Taking into account paleographical, ortho-graphical and linguistic aspects, the author tries to reconstruct the original manu-script from which both fragments derive, reaching the conclusion that it was writ-ten in the first quarte

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14

Calbi, Maurizio. "“This England”: Re-Visiting Shakespearean Landscapes and Mediascapes in John Akomfrah’s The Nine Muses (2010)." Multicultural Shakespeare: Translation, Appropriation and Performance 15, no. 30 (2017): 59–75. http://dx.doi.org/10.1515/mstap-2017-0005.

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The paper will offer a reading of John Akomfrah’s The Nine Muses (2010), a 90-minute experimental feature film that has been defined as “one of the most vital and original artistic responses to the subject of immigration that British cinema has ever produced” (Mitchell). It will focus on the multifarious ways in which the film makes the “canonical” literary material that it incorporates, including Shakespeare, interact with rarely seen archival material from the BBC regarding the experience of Caribbean and South Asian immigrants in 1950s and 1960s Britain. It will argue that through this inte

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Kim, Chi Heon. "Postoperative Longitudinal Outcomes in Patients with Residual Disc Fragments after Percutaneous Endoscopic Lumbar Discectomy." January 2018 1, no. 21;1 (2018): E457—E466. http://dx.doi.org/10.36076/ppj.2018.4.e457.

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Background: Residual disc fragments are observed on immediate postoperative magnetic resonance imaging (MRI) in 2.8–15% of patients after percutaneous endoscopic lumbar discectomy (PELD). Considering the known postoperative longitudinal outcomes in patients with residual disc tissue, a ‘watchful waiting’ strategy may be preferable to immediate re-operation in patients with asymptomatic residual disc material. Objectives: The aim of the present study was to compare the longitudinal clinical outcomes between PELD patients in whom the complete removal of disc fragments was achieved (complete grou

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Pio, I. D. S. L., A. L. Lavor, C. M. D. Damasceno, P. M. N. Menezes, F. S. Silva, and G. L. A. Maia. "Traditional knowledge and uses of medicinal plants by the inhabitants of the islands of the São Francisco river, Brazil and preliminary analysis of Rhaphiodon echinus (Lamiaceae)." Brazilian Journal of Biology 79, no. 1 (2019): 87–99. http://dx.doi.org/10.1590/1519-6984.177447.

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Abstract This study aimed to carry out an ethnobotanical survey of medicinal plants used by inhabitants of the Rodeadouro Island, Jatoba Island and Massangano Island, located in The Submedium São Francisco River Valley. Also phytochemicals and preliminary pharmacological tests were performed to species most cited by the community. Ethnobotanical data were collected through observation visits and semi-structured interviews with 12 key informants. We calculated the relative importance (RI), the percentage of agreement related to the main uses (cAMU) and use value (UV). The aerial parts of Rhaphi

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17

Maidana, Silvina S., Samuel Miño, Romina M. Apostolo, Gabriel A. De Stefano, and Sonia A. Romera. "A new molecular method for the rapid subtyping of bovine herpesvirus 1 field isolates." Journal of Veterinary Diagnostic Investigation 32, no. 1 (2020): 112–17. http://dx.doi.org/10.1177/1040638719898692.

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Bovine herpesvirus 1 (BoHV-1) causes several clinical syndromes in cattle worldwide. There are 3 subtypes of BoHV-1: 1.1, 1.2a, and 1.2b. Several molecular methods are commonly used in the detection and characterization of BoHV-1. Among them, restriction endonuclease analysis (REA) and single-nucleotide polymorphism (SNP) analysis of the complete viral genome allow classification of BoHV-1 into different subtypes. However, developing countries need simpler and cheaper screening assays for routine testing. We designed a standard multiplex PCR followed by a REA assay allowing straightforward sub

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18

Goebs, Katja. "A FUNCTIONAL APPROACH TO EGYPTIAN MYTH AND MYTHEMES." Journal of Ancient Near Eastern Religions 2, no. 1 (2002): 27–59. http://dx.doi.org/10.1163/156921202762733879.

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AbstractThe article re-examines the definition of the Egyptian mythical tradition as notably different from that of other cultures. In particular, the supposed late development of Egyptian (narrative) myths, which has traditionally been inferred from the variability in both content and form of mythical fragments/mythemes in different contexts, is re-evaluated. It is argued that the form a myth or mytheme takes is dependent on the function of the context in which it is used, with the (structural) relationships between actors (or actors and objects) taking precedence over their identity, which i

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19

Vukovic, Jasna. "Pottery shreds as tools in late Neolithic Vinca." Starinar, no. 63 (2013): 191–207. http://dx.doi.org/10.2298/sta1363191v.

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Over the last few years, careful analysis of a large quantity of ceramic material found during excavation at Vinca has identified a significant number of ceramic fragments with clear use-wear traces. Recycled fragments of ceramic vessels are characterized by the presence of damage and deformation caused by abrasive processes i.e. contact of the fragments with hard material. All recorded recycled fragments can generally be divided into two basic groups: fragments representing tools with a working edge, and fragments with traces of abrasion on their outer surface. The largest group comprises fra

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Santoso, Tri Joko, Sri H. Hidayat, M. Herman, H. Aswidinnoor, and Sudarsono Sudarsono. "Identitas dan Keragaman Genetik Begomovirus yang Berasosiasi dengan Penyakit Keriting pada Tomat Berdasarkan Teknik Polymerase Chain Reaction (PCR)- Restriction Fragment Length Polymorphism (RFLP)." Jurnal AgroBiogen 4, no. 1 (2016): 9. http://dx.doi.org/10.21082/jbio.v4n1.2008.p9-17.

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<p>Begomoviruses, members of the Geminivirus,<br />are considered as emerging plant viruses. This was due to<br />the increasing incidences and severities of the diseases in a<br />number of economically important crops, including tomato.<br />Genetic diversities of the Begomovirus isolates infecting<br />tomato (Lycopersicon esculentum) of several areas in Indonesia<br />were analyzed by using Polymerase Chain Reaction-<br />Restriction Fragment Length Polymorphism (PCR-RFLP)<br />technique. A 1500 base pairs of PCR fragments amplified by&

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Cimmino, Nicola, Giorgio Isoletta, Roberto Opromolla, et al. "Tuning of NASA Standard Breakup Model for Fragmentation Events Modelling." Aerospace 8, no. 7 (2021): 185. http://dx.doi.org/10.3390/aerospace8070185.

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The continuous growth of space debris motivates the development and the improvement of tools that support the monitoring of a more and more congested space environment. Satellite breakup models play a key role to predict and analyze orbital debris evolution, and the NASA Standard Breakup Model represents a widely used reference, with current activities relevant to its evolution and improvements especially towards fragmentation of small mass spacecraft. From an operational perspective, an important point for fragmentation modelling concerns the tuning of the breakup model to achieve consistency

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Giovannini, E. C. "WORKFLOW FOR AN EVIDENCE-BASED VIRTUAL RECONSTRUCTION: THE MARBLES OF THE CIBORIUM OF THE EARLY MEDIEVAL MONTE SORBO CHURCH." ISPRS - International Archives of the Photogrammetry, Remote Sensing and Spatial Information Sciences XLIII-B2-2020 (August 14, 2020): 1395–402. http://dx.doi.org/10.5194/isprs-archives-xliii-b2-2020-1395-2020.

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Abstract. The paper presents a practical approach to define a hypothetical virtual reconstruction of the ciborium and pergola of Monte Sorbo's Church. The high accuracy of archaeological fragments, digitally acquired, allowed to hypothesize the entire sequences of decorations of architectural elements and to define their geometrical rules. Starting from the acquisition to the 3d modelling phase, the proposed virtual reconstruction combines original fragments with their lost parts modelled in a digital environment.Digital technologies offer nowadays the great possibility to visualize and unders

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23

Tema, E., S. Pavlides, and D. Kondopoulou. "Late bronze age pottery as indicator of the deposition temperatures of the Minoan pyroclastic products, Santorini, Greece." Bulletin of the Geological Society of Greece 47, no. 3 (2016): 1289. http://dx.doi.org/10.12681/bgsg.10904.

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The Minoan eruption of Santorini volcano (Greece) took place in the Late Bronze Age (17th century BC) and produced a great volume of volcanic products that covered the whole island and buried every human settlement under meters of pyroclastic deposits. In this study we used thermal analysis of the magnetic remanence carried by pottery fragments buried under the pyroclastic deposits in order to estimate the thermal effect of the Minoan volcanic products on the pre-eruption habitation level. A total of 70 samples, prepared from 45 independent pottery fragments, have been studied. Samples were co

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Essomba, Terence, and Sinh Nguyen Phu. "Kinematic design of a hybrid planar-tripod mechanism for bone reduction surgery." Mechanics & Industry 21, no. 4 (2020): 403. http://dx.doi.org/10.1051/meca/2020030.

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In the most severe cases of longitudinal bone fractures such as femur, tibias, humerus etc., the bone can be completely separated into two fragments. In order to guarantee the re-ossification of the bone, it is required to reposition the bone fragments together. This process requires a delicate surgery called “bone reduction surgery”. The most advanced technique relies on the use of a robotic manipulator to reposition the bone fragments with higher precision and stability than manual surgeries. The present work introduces the kinematic design of a new hybrid mechanical architecture to perform

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ČIAMPOR JR., FEDOR, and JÁN KODADA. "Taxonomy of the Oulimnius tuberculatus species group (Coleoptera: Elmidae) based on molecular and morphological data." Zootaxa 2670, no. 1 (2010): 59. http://dx.doi.org/10.11646/zootaxa.2670.1.4.

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Two mitochondrial (cytochrome oxidase subunit I, 16S RNA) and one nuclear (18S RNA) gene fragments were used to analyse relationships within the Oulimnius tuberculatus species group. The results of molecular data analysis are compared with morphological characters, and the distributional patterns and taxonomic status of recognized taxa are addressed. Based on molecular data, Oulimnius perezi is re-validated as a distinct species, and the discovery of a possible new species from northwest Italy is reported. The possible origins and times of speciation within the species group are discussed.

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Santos, Gilson José. "De re rustica de Columela e Geórgicas IV de Virgílio: estudo crítico-comparativo." Nuntius Antiquus 10, no. 2 (2014): 71–88. http://dx.doi.org/10.17851/1983-3636.10.2.71-88.

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In the study we analyse comparatively selected fragments from Georgics IV and De re rustica (Book IX) in order to understand a little better the linguistic-literary treatment due to the distinct generic orientations the authors deliver to their works. The dominating compounding and stylistic characteristics in the texts are studied and are used as instruments to analyse (a) the constitutive particularities of the genders, didactic poetry and technical treatise (in prose), and (b) the aspects related to the construction of the particular meanings in the works. The theoretical reference marks fo

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Manchanda, Rahul, Steven R. Hirsch, and Thomas R. E. Barnes. "Criteria for Evaluating Improvement in Schizophrenia in Psychopharmacological Research (With Special Reference to Gamma Endorphin Fragments)." British Journal of Psychiatry 153, no. 3 (1988): 354–58. http://dx.doi.org/10.1192/bjp.153.3.354.

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A review of treatment trials with DTγE revealed widely discrepant results. Relevant variables were the variety of measures employed for monitoring psychotic symptoms, and the different criteria used to judge the degree of improvement. The authors suggest a uniform outcome criterion for early trials of new treatments, which would generate more consistent and comparable results between studies, and give a stronger indication of the value of the treatment under test. When the data from the various treatment trials of DTγE were re analysed, applying a uniform outcome criterion of improvement of a

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Gram, Jørgen, Jørgen Jespersen, Moniek de Maat, and Else-Marie Bladbjerg. "Internal Quality Control of PCR-based Genotyping Methods in Research Studies and Patient Diagnostics." Thrombosis and Haemostasis 87, no. 05 (2002): 812–16. http://dx.doi.org/10.1055/s-0037-1613089.

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SummaryGenetic analyses are increasingly integrated in the clinical laboratory, and internal quality control programmes are needed. We have focused on quality control aspects of selected polymorphism analyses used in thrombosis research. DNA was isolated from EDTA-blood (n = 500) by ammonium acetate precipitation and analysed for 18 polymorphisms by polymerase chain reaction (PCR), i. e. restriction fragment length polymorphisms, allele specific amplification, or amplification of insertion/deletion fragments. We evaluated the following aspects in the analytical procedures: sample handling and

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Arinchev, S. V. "Dynamics Analysis of Laser 2D Cutting of a Large Space Debris Object Under Zero-Gravity Conditions." Proceedings of Higher Educational Institutions. Маchine Building, no. 12 (729) (December 2020): 50–61. http://dx.doi.org/10.18698/0536-1044-2020-12-50-61.

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In this paper two independent objects in low-earth orbit are analysed. The first object is a large piece of space debris containing fragments that need to be extracted and re-used. The second object is a space debris collector equipped with a laser powerful enough to destruct any element of the space debris in an infinitely small period of time. It is accepted that the switch on and switch off times of the laser beam are infinitely small. It is supposed that the objects are moving in near orbits and the difference between their velocities is small. The object protects itself by applying attitu

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Gedye, Craig, Danylo Sirskyj, Nazleen Carol Lobo, et al. "Essential experimental steps and estimates of renal carcinoma initiating cells." Journal of Clinical Oncology 32, no. 15_suppl (2014): 11127. http://dx.doi.org/10.1200/jco.2014.32.15_suppl.11127.

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11127 Background: Rare cancer stem cells (CSC), proposed to be solely responsible for tumor propagation and re-initiation, are functionally identified as tumor-initiating cells (TIC) from ex vivo tumors using xenotransplantation and clonogenic limiting dilution assays (LDA). TIC have not previously been described from ex vivohuman clear cell renal cell carcinoma (ccRCC). Methods: Primary human ccRCC samples (n=120) from patients undergoing nephrectomy were processed and implanted as subcapsular fragments or cell suspension injection LDAs with Matrigel in NOD/SCID/IL2Rγ-/- (NSG) mice, and obser

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Li, K. G., G. P. Pogossian, A. K. Moldagulova, et al. "Identification of L. casei and L. rhamnosus in the dairy products using Real-Time PCR assay." South Asian Journal of Experimental Biology 4, no. 5 (2015): 222–25. http://dx.doi.org/10.38150/sajeb.4(5).p222-225.

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Lactobacilli are essential and important biological objects used in food pro-duction and medicine. One of the sufficient problems is fast, reliable and highly specific identification of lactobacilli in the scientific research and cur-rent production control. We represent two species-specific real-time PCR in the present study to discriminate L. rhamnosus and L. casei basing on the unique peptidoglycan-hydrolase genes p40 and p75 respectively. PCR pri-mers and probes were designed to provide high specificity discrimination via high temperature of PCR annealing stage. High efficiency of the reac

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Leong, Ka Wai, Fangyan Yu, Viktor A. Adalsteinsson, et al. "A nuclease-polymerase chain reaction enables amplification of probes used for capture-based DNA target enrichment." Nucleic Acids Research 47, no. 22 (2019): e147-e147. http://dx.doi.org/10.1093/nar/gkz870.

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Abstract DNA target enrichment via hybridization capture is a commonly adopted approach which remains expensive due in-part to using biotinylated-probe panels. Here we provide a novel isothermal amplification reaction to amplify rapidly existing probe panels without knowledge of the sequences involved, thereby decreasing a major portion of the overall sample preparation cost. The reaction employs two thermostable enzymes, BST-polymerase and duplex-specific nuclease DSN. DSN initiates random ‘nicks’ on double-stranded-DNA which enable BST to polymerize DNA by displacing the nicked-strand. Displ

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Greinacher, A., U. Liebenhoff, V. Kiefel, P. Presek, and C. Mueller-Eckhardt. "Heparin-Associated Thrombocytopenia: The Effects of Various Intravenous lgG Preparations on Antibody Mediated Platelet Activation - A Possible New Indication for High Dose i.v. IgG." Thrombosis and Haemostasis 71, no. 05 (1994): 641–45. http://dx.doi.org/10.1055/s-0038-1642496.

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SummaryThe immunologic type of heparin-associated thrombocytopenia (HAT) is caused by antibodies which activate platelets via the Fc-re- ceptor in the presence of polysulfated oligosaccharides. The antigen is formed by a releasable platelet protein (in many cases PF4) complexed to heparin. Since the role of GP Ilb/IIIa in platelet activation by HAT antibodies is controversial, we investigated platelet activation by antibodies related to HAT. We used normal platelets and platelets from a patient with Glanzmann’s thrombasthenia (GT) lacking GP Ilb/IIIa. Heparin and sera from patients with HAT st

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Gauthier, N. W., J. Polashock, T. T. Veetil, R. R. Martin, and J. Beale. "First Report of Blueberry Mosaic Disease Caused by Blueberry mosaic associated virus in Kentucky." Plant Disease 99, no. 3 (2015): 421. http://dx.doi.org/10.1094/pdis-09-14-0946-pdn.

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In 2011, a grower in Casey County, Kentucky, observed persistent yellow, green, and red mosaic patterns on leaves of highbush blueberry plants. Twenty-three randomly-scattered cv. Bluecrop plants out of approximately 1,400 5-year-old plants showed symptoms, with coverage on each plant ranging from 5 to 100%. Asymptomatic canes bloomed normally and produced fruit; affected canes were stunted and did not bloom. These symptoms are generally consistent with those described for blueberry mosaic disease (BMD) (1,3), the casual agent of which is Blueberry mosaic associated virus (BlMaV) (4). All plan

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Thuong, Ho Thi, Nguyen Thi Thom, Nguyen Thi Tra, et al. "Production of monoclonal antibody against Ompa protein of Candidatus Liberibacter asiaticus causing citrus greening disease." Vietnam Journal of Biotechnology 18, no. 3 (2020): 529–41. http://dx.doi.org/10.15625/1811-4989/18/3/14634.

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Citrus Greening, also known as HuangLongbing (HLB), is considered one of the most dangerous citrus diseases, and limiting the production of citrus trees all over the world. Production of antibodies against Ompa protein of Candidatus Liberibacter asiaticus (CLas) for detection of citrus greening disease is considered as promising research direction. In this study, for the purpose of producting antibodies against Ompa of CLas, we firstly used the camel VHH antibody library for screening VHH antibodies against Ompa using phage-display technique. Next, phages which had strong interaction with Ompa

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Rutgers, Leonard V., Arie F. M. De Jong, and Klaas van der Borg. "Radiocarbon Dates from the Jewish Catacombs of Rome." Radiocarbon 44, no. 2 (2002): 541–47. http://dx.doi.org/10.1017/s0033822200031891.

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This paper reports on the first chronological assessment of the Jewish Catacombs of the ancient Rome performed by accelerator mass spectrometry (AMS) dating of small-size charcoal fragments scattered in the mortar used for sealing off the graves in the Villa Torlonia Catacomb complex. The significance of the obtained 14C readings has been carefully evaluated by taking into consideration the known technologies of quicklime production during Roman and recent times. The new data are of great concern for providing evidence that the Jewish catacombs were used for burial since the first century AD,

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Giuntoli, Francesco, Pierre Lanari, Marco Burn, Barbara Eva Kunz, and Martin Engi. "Deeply subducted continental fragments – Part 2: Insight from petrochronology in the central Sesia Zone (western Italian Alps)." Solid Earth 9, no. 1 (2018): 191–222. http://dx.doi.org/10.5194/se-9-191-2018.

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Abstract. Subducted continental terranes commonly comprise an assembly of subunits that reflect the different tectono-metamorphic histories they experienced in the subduction zone. Our challenge is to unravel how, when, and in which part of the subduction zone these subunits were juxtaposed. Petrochronology offers powerful tools to decipher pressure–temperature–time (P–T–t) histories of metamorphic rocks that preserve a record of several stages of transformation. A major issue is that the driving forces for re-equilibration at high pressure are not well understood. For example, continental gra

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Driscoll, Stephen T. "Excavations at Glasgow Cathedral: A Preliminary Report on the Archaeological Discoveries made in 1992-93." Glasgow Archaeological Journal 17, no. 1 (1991): 63–76. http://dx.doi.org/10.3366/gas.1991.17.17.63.

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Summary Extensive archaeological excavations were conducted in advance of the installation of a new healing and electrical system for the Cathedral. Trenches were located where new ducts were to be installed below the floor in the Nave, the Choir, the Crypt and the Session Room. These trenches produced evidence for both of the documented 12th century cathedrals which preceded the existing building. The early cathedrals were represented by in situ masonry and decorated fragments of masonry which had been re-used in the thirteenth century works. Traces of activity pre-dating the 13th century wer

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Lin, Joanne, Stacey L. Lee, Anna M. Russell, et al. "A structure-based engineering approach to abrogate pre-existing antibody binding to biotherapeutics." PLOS ONE 16, no. 7 (2021): e0254944. http://dx.doi.org/10.1371/journal.pone.0254944.

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Development of biotherapeutics is hampered by the inherent risk of immunogenicity, which requires extensive clinical assessment and possible re-engineering efforts for mitigation. The focus in the pre-clinical phase is to determine the likelihood of developing treatment-emergent anti-drug antibodies (TE-ADA) and presence of pre-existing ADA in drug-naïve individuals as risk-profiling strategies. Pre-existing ADAs are routinely identified during clinical immunogenicity assessment, but their origin and impact on drug safety and efficacy have not been fully elucidated. One specific class of pre-e

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Bisconti, Michelangelo, Olivier Lambert, and Mark Bosselaers. "Revision of “Balaena” belgica reveals a new right whale species, the possible ancestry of the northern right whale, Eubalaena glacialis, and the ages of divergence for the living right whale species." PeerJ 5 (June 27, 2017): e3464. http://dx.doi.org/10.7717/peerj.3464.

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In 1941, Abel established Balaena belgica based on a series of fused cervical vertebrae and citing other cranial fragments from the late Neogene of the Antwerp harbor (northern Belgium). Later, Plisnier-Ladame & Quinet (1969) added a neurocranium and other skeletal remains from the same area to this species. Recently, the neurocranium was re-assigned to the genus Eubalaena thanks to newer phylogenetic analyses. Here, a new description is provided of materials previously assigned to “Balaena” belgica together with taxonomic revisions. Our work suggests that the cervical complex originally d

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LI, XIN-RAN, MENG LI, and ZONG-QING WANG. "Preliminary molecular phylogeny of beetle cockroaches (Diploptera) and notes on male and female genitalia (Blattodea: Blaberidae: Diplopterinae)." Zootaxa 4320, no. 3 (2017): 523. http://dx.doi.org/10.11646/zootaxa.4320.3.7.

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The beetle cockroach, or genus Diploptera Saussure, has been reviewed recently, with unresolved issues remaining. New materials facilitated a molecular phylogenetic study and further comparisons of male and female genitalia among known species. We performed phylogenetic estimates based on two mitochondrial DNA fragments: 657 bases of COI gene and 376 bases of 16S rRNA gene. We used codon model and doublet model (secondary structure) for COI and 16S respectively, and the predicted secondary structure of sequenced 16S fragment is illustrated. The phylogeny revealed that 1) D. bicolor Hanitsch is

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Bradeen, James M., and Philipp Simon. "Toward Characterization of and Breeder-friendly Molecular Markers for Genes Affecting Carotene accumulation in Carrot (Daucus carota)." HortScience 32, no. 3 (1997): 512D—512. http://dx.doi.org/10.21273/hortsci.32.3.512d.

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The Y2 locus conditions α- and β-carotene accumulation in the xylem (core) of carrot roots. The dominant allele suppresses carotene, but not xanthophyll accumulation, resulting in yellow-cored roots. Individuals homozygous for the recessive allele are rich in carotenes and are therefore orange-cored. Increased consumer interest in high carotene produce requires improved understanding of carotene biosynthesis and color development and more-efficient breeding techniques. We examined 103 F2 individuals generated from inbred populations differing in core carotene content. Bulked segregant analysis

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Iaccarino Idelson, A., S. Pannuzi, A. Brunetto, et al. "USE OF 3D TECHNOLOGIES WITHIN THE CONSERVATION OF THE ANCIENT WINDOWS OF THE BASILICA OF S. SABINA IN ROME. CONSTRUCTION OF EXHIBITION STANDS IN CARBON COMPOSITE ON A MILLED STRUCTURE." ISPRS - International Archives of the Photogrammetry, Remote Sensing and Spatial Information Sciences XLII-5/W1 (May 16, 2017): 593–98. http://dx.doi.org/10.5194/isprs-archives-xlii-5-w1-593-2017.

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Rare and precious window elements from the Paleochristian Basilica of Saint Sabina in Rome, made of plaster gypsum with translucent selenite used as glass for light transmission, were discovered by Antonio Muñoz during the restoration of the building at the beginning of the 20th c. Originally standing within the stone window frame, were then mounted on wood planks with screws for holding together the scattered fragments. The surfaces were covered with grime and the selenite elements were blinded by the wooden supports. <br><br> During the recent conservation treatment at ISCR, trac

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Almeida, Luiza Helena Silva de, Natália Gomes e. Silva Leonardo, Ana Paula Neutzling Gomes, Luciano Giardino, Erick Miranda Souza, and Fernanda Geraldo Pappen. "Pulp Tissue Dissolution Capacity of Sodium Hypochlorite Combined with Cetrimide and Polypropylene Glycol." Brazilian Dental Journal 24, no. 5 (2013): 477–81. http://dx.doi.org/10.1590/0103-6440201302041.

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This study evaluated the influence of the addition of cetrimide and polypropylene glycol to sodium hypochlorite (NaOCl) on its capacity to dissolve pulp tissue. Bovine pulp fragments with standardized weight and volume were immersed for 5, 15 and 30 min in 2 mL of NaOCl and Hypoclean (NaOCl added with cetrimide and polypropylene glycol) solutions at 5.25%, 2.5%, 1%, 0.5% and 0.25% and afterwards re-weighted. Distilled water was used as a control. The percentage of tissue loss was considered for statistical analysis (univariate ANOVA, SPSS, v. 17.0) at 5% significance level. There was no tissue

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Barnwell, J. W., M. E. Nichols, and P. Rubinstein. "In vitro evaluation of the role of the Duffy blood group in erythrocyte invasion by Plasmodium vivax." Journal of Experimental Medicine 169, no. 5 (1989): 1795–802. http://dx.doi.org/10.1084/jem.169.5.1795.

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A short-term in vitro culture system that allows for significant re-invasion of target erythrocytes by Plasmodium vivax was used to study the role of the Duffy blood group antigen as a ligand for merozoite invasion by this human malaria species. Using human Duffy-positive and -negative erythrocytes, various primate erythrocytes, enzymatic modification of erythrocytes, and mAb that defines a new Duffy determinant (Fy6) we conclude that the erythrocyte glycoprotein carrying Duffy determinants is required as a ligand for the invasion of human erythrocytes by P. vivax merozoites. Blockade of invas

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H Nazht, Humam. "Advantages and Disadvantages of using Food Grate Stainless Steel Rods for Internal Fixation of Femoral Transverse Fractures in Rabbits (Review Study)." Open Access Journal of Veterinary Science & Research 5, no. 2 (2020): 1–7. http://dx.doi.org/10.23880/oajvsr-16000198.

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The present study designed to focus on the advantages and disadvantages of using food grate stainless steel rods (FGSR) as internal fixation methods for induced transverse fractures in the mid shift of the femoral bones in rabbits.200 cases were collected from 2007 to 2020, all these cases were employed to induced transverse fractures in the mid shift of femoral bone, 100 of them used the rods for internal fixation, 80 of the cases used the rods for fixation the natural xen- bony implantation from sheep or calves, and the others 20 cases used the rods for internal fixation of the synthetics na

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Zoltner, Martin, Paul K. Fyfe, Tracy Palmer, and William N. Hunter. "Characterization of Staphylococcus aureus EssB, an integral membrane component of the Type VII secretion system: atomic resolution crystal structure of the cytoplasmic segment." Biochemical Journal 449, no. 2 (2012): 469–77. http://dx.doi.org/10.1042/bj20121209.

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The Type VII protein translocation/secretion system, unique to Gram-positive bacteria, is a key virulence determinant in Staphylococcus aureus. We aim to characterize the architecture of this secretion machinery and now describe the present study of S. aureus EssB, a 52 kDa bitopic membrane protein essential for secretion of the ESAT-6 (early secretory antigenic target of 6 kDa) family of proteins, the prototypic substrate of Type VII secretion. Full-length EssB was heterologously expressed in Escherichia coli, solubilized from the bacterial membrane, purified to homogeneity and shown to be di

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Ward, John O. "Quintilian and the Rhetorical Revolution of the Middle Ages." Rhetorica 13, no. 3 (1995): 231–84. http://dx.doi.org/10.1525/rh.1995.13.3.231.

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Abstract: It is often asserted nowadays that the medieval period “fragmented” the classical rhetorical inheritance, while the Renaissance restored it to its former coherence. The story of the assimilation in the Middle Ages and Renaissance of Quintilian's Institutes of Oratory is examined here in order to demonstrate the problems inherent in such a position. It is argued that the full utilization of the text of Quintilian's Institutes of Oratory in the Renaissance, along with the discrediting of the Ad Herennium (as a work of Cicero) that is associated with the name of Raffaello Regio in the l

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Höhne-Zell, B., G. Knoll, U. Riedel-Gras, W. Hofer, and H. Plattner. "A cortical phosphoprotein (‘PP63’) sensitive to exocytosis triggering in Paramecium cells. Immunolocalization and quenched-flow correlation of time course of dephosphorylation with membrane fusion." Biochemical Journal 286, no. 3 (1992): 843–49. http://dx.doi.org/10.1042/bj2860843.

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We had previously shown that a phosphoprotein of 63 kDa (‘PP63’) is rapidly and selectively dephosphorylated during synchronous (less than or equal to 1 s) trichocyst exocytosis in Paramecium cells and then rephosphorylated within less than or equal to 1 min [Zieseniss & Plattner (1985) J. Cell Biol. 101, 2028-2035]. Using a new quenched-flow device, we now find a strict correlation between PP63 dephosphorylation and the process of membrane fusion, both occurring within 80 ms. Uptake of 32P over 90 min, followed by exocytosis and rephosphorylation for 1 min, results in a rather selective p

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Wilt, F. H. "Determination and morphogenesis in the sea urchin embryo." Development 100, no. 4 (1987): 559–76. http://dx.doi.org/10.1242/dev.100.4.559.

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The study of the sea urchin embryo has contributed importantly to our ideas about embryogenesis. This essay re-examines some issues where the concerns of classical experimental embryology and cell and molecular biology converge. The sea urchin egg has an inherent animal-vegetal polarity. An egg fragment that contains both animal and vegetal material will produce a fairly normal larva. However, it is not clear to what extent the oral-aboral axis is specified in embryos developing from meridional fragments. Newly available markers of the oral-aboral axis allow this issue to be settled. When equa

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