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Soon, Wilson Wei-Chwen. "Near real-time extract, transform and load." [Denver, Colo.] : Regis University, 2007. http://165.236.235.140/lib/WSoon2007.pdf.
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Ghosh, Sushmita. "Real time data acquisition for load management." Thesis, Virginia Tech, 1985. http://hdl.handle.net/10919/45726.
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Demand for Data Transfer between computers has increased ever since the introduction of Personal Computers (PC). Data Communicating on the Personal Computer is much more productive as it is an intelligent terminal that can connect to various hosts on the same I/O hardware circuit as well as execute processes on its own as an isolated system.
Yet, the PC on its own is useless for data communication. It requires a hardware interface circuit and software for controlling the handshaking signals and setting up communication parameters. Often the data is distorted due to noise in the line. Such transmission errors are imbedded in the data and require careful filtering.
The thesis deals with the development of a Data Acquisition system that collects real time load and weather data and stores them as historical database for use in a load forecast algorithm in a load management system. A filtering technique has been developed here that checks for transmission errors in the raw data. The microcomputers used in this development are the IBM PC/XT and the AT&T 3B2 supermicro computer.Master of Science
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SOTOMAYOR, JUAN MANUEL GIRAO. "EVALUATION OF LOAD-SETTLEMENT BEHAVIOR OF COCONUT FIBERS REINFORCED SAND UNDER PLATE LOAD TESTS IN REAL SCALE." PONTIFÍCIA UNIVERSIDADE CATÓLICA DO RIO DE JANEIRO, 2014. http://www.maxwell.vrac.puc-rio.br/Busca_etds.php?strSecao=resultado&nrSeq=25290@1.
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PONTIFÍCIA UNIVERSIDADE CATÓLICA DO RIO DE JANEIROCOORDENAÇÃO DE APERFEIÇOAMENTO DO PESSOAL DE ENSINO SUPERIOR
PROGRAMA DE EXCELENCIA ACADEMICA
Este estudo avaliou a influência da inclusão de fibra de coco como reforço de solo. A fibra de coco utilizada foi obtida por processo mecânico na empresa ECOFIBRA, que possui uma parceria com a Companhia de Limpeza Urbana da cidade do Rio de Janeiro (COMLURB) em projeto piloto de coleta seletiva das cascas de coco verde. Foi realizada uma comparação entre a areia pura e a areia reforçada com fibras de coco para avaliar o comportamento carga-recalque por meio de ensaios de placa em verdadeira grandeza. O objetivo é usar a fibra de coco que normalmente é descartada em grande quantidade, após o consumo do fruto. Procura-se estabelecer padrões de comportamento que possam explicar a influência da inclusão de fibras aleatoriamente distribuídas e de fibras colocadas no formato de manta entre as camadas de solo. Os ensaios foram realizados utilizando uma densidade relativa padrão de 50 por cento e um teor de umidade de 10 por cento. Foi observado nas curvas carga-recalque que o reforço de fibra em manta conseguiu uma diminuição maior do recalque, mas com um maior fissuramento superficial, enquanto que o reforço com as fibras colocadas aleatoriamente conseguiu uma menor redução dos recalques, mas uma melhor inibição da propagação de fissuras. Ambos os resultados, comparados com a areia sem adição de fibras, demostraram uma maior resistência e redução do recalque ocorrido na areia pura. Os resultados são satisfatórios para a aplicação de reforço de solo com fibras de coco em camadas de aterros sanitários e aterros sobre solos moles, dentre outros, dando um fim mais nobre para este material e obtendo-se uma solução para o problema da acumulação da fibra de coco, devido ao grande consumo existente na cidade de Rio de Janeiro.
This study evaluated the influence of the inclusion of coconut fiber as reinforcement of soil. The coconut fiber used is obtained by a mechanical process in ECOFIBRA Company, which has a partnership with the Urban Cleaning Company of the city of Rio de Janeiro (COMLURB) in a pilot project about separate collection of green coconut. Sandy soil reinforced and unreinforced were compared, evaluating load-settlement behavior through real-scale plate load testing. The aim is to use the coconut fiber, discarded in large quantities after consumption of the fruit. It seeks to establish patterns of behavior that may explain the influence of the inclusion of fibers randomly (individual) and as a reinforcing mat (composed) between layers of soil. The tests were performed using as a standard, a relative density of 50 per cent and a humidity content of 10 per cent. The load-settlement curves showed a better stress distribution using fiber rug but a greater degree of surface fissuring was noted. Instead using randomization distribution, stress distribution was lower but was inhibited surface fissuring propagation; both results were compared with soil without addition of fibers increased resistance to settlement. The results are satisfactory for an application of reinforcement layers of soil in landfills and platforms over soft ground, giving a nobler end to this material, trying to give a solution to the problem of accumulation due to the large consumption existing in Rio de Janeiro city.
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Cunningham, Ian Joseph. "Load balancing schemes for distributed real-time interactive virtual world simulations." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2000. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape3/PQDD_0016/MQ56681.pdf.
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Sebitosi, A. B. "Load compensation : design of a real time analysis and control device." Master's thesis, University of Cape Town, 2001. http://hdl.handle.net/11427/5107.
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Includes bibliographical references.The aim of this thesis is to produce a load compensator for a three-phase system. It should be simple, accurate and affordable. The three-phase load compensator design is based on a more recent definition of power factor. Attempts to establish a universally acceptable definition can be traced as early as 1920 at the 36th Annual convention of the American Institution of Electrical Engineers. Subsequently, a number of definitions have been adopted by different scholars. Each definition can lead to a different compensator solution. This problem, for example, is illustrated by Eammanuel [25].
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Christophersen, Jon Petter. "Battery state-of-health assessment using a near real-time impedance measurement technique under no-load and load conditions." Diss., Montana State University, 2011. http://etd.lib.montana.edu/etd/2011/christophersen/ChristophersenJ0511.pdf.
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The reliability of battery technologies has become a critical issue as the United States seeks to reduce its dependence on foreign oil. One of the significant limitations of in-situ battery health and reliability assessments, however, has been the inability to rapidly acquire information on power capability during aging. The Idaho National Laboratory has been collaborating with Montana Tech of the University of Montana and Qualtech Systems, Incorporated, on the development of a Smart Battery Status Monitor. This in-situ device will track changes in battery performance parameters to estimate its state-of-health and remaining useful life. A key component of this onboard monitoring system will be rapid, in-situ impedance measurements from which the available power can be estimated. A novel measurement technique, known as Harmonic Compensated Synchronous Detection, has been developed to acquire a wideband impedance spectrum based on an input sum-of-sines signal that contains frequencies separated by octave harmonics and has a duration of only one period of the lowest frequency. For this research, studies were conducted with high-power lithium-ion cells to examine the effectiveness and long-term impact of in-situ Harmonic Compensated Synchronous Detection measurements. Cells were cycled using standardized methods with periodic interruptions for reference performance tests to gauge degradation. The results demonstrated that in-situ impedance measurements were benign and could be successfully implemented under both no-load and load conditions. The acquired impedance spectra under no-load conditions were highly correlated to the independently determined pulse resistance growth and power fade. Similarly, the impedance measurements under load successfully reflected changes in cycle-life pulse resistance at elevated test temperatures. However, both the simulated and measured results were corrupted by transient effects and, for the under-load spectra, a bias voltage error. These errors mostly influenced the impedance at low frequencies, while the mid-frequency charge transfer resistance was generally retained regardless of current level. It was further demonstrated that these corrupting influences could be minimized with additional periods of the lowest frequency. Therefore, the data from these studies demonstrate that Harmonic Compensated Synchronous Detection is a viable in-situ impedance measurement technique that could be implemented as part of the overall Smart Battery Status Monitor.
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Davis, A. G. W. "A transputer ring network for real time distributed control applications." Thesis, University of Nottingham, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.260571.
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Bjerke, Hanne. "Revealing Causes of Restrictions by Signatures in Real-Time Hook Load Signals." Thesis, Norges teknisk-naturvitenskapelige universitet, Institutt for petroleumsteknologi og anvendt geofysikk, 2013. http://urn.kb.se/resolve?urn=urn:nbn:no:ntnu:diva-22906.
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Downhole restrictions are causing non-productive time, which represents large economic losses. Knowing the cause of the restriction in order to implement the correct remedies are crucial for preventing extensive cleaning activities or even stuck pipe. Using the hook load signal to find special signatures for the different causes of restriction could be a solution to quick recognition of restriction type.In the wells studied it was found 22 cases of restriction, which could be divided into 5 main groups of causes; unstable wellbore, ledges, cuttings accumulation, differential sticking and local dogleg. One incident from each group was chosen for an extensive post-event analysis for the purpose of strengthening the hypothesis of the cause.The results from the study has shown that it was necessary to simplify the analysis in to two main types of hook load restriction signatures; fixed and moveable. For the physical interpretation of the two signatures, fixed and moveable, hook load signals from ledge and cuttings bed were used respectively. These were assumed to be good representatives for the two main types, and the signals proved to coincide with the physical explanation valid for them. The two groups were created based on the clear differences in the hook load signals between ledges and cuttings accumulation visible in the post-event analysis. Both of these causes of restriction have a very clear physical explanation; when the drill string encounters a ledge it will stop moving and is thereby fixed to one position in the well. On the other hand, cuttings downhole is moveable and is able to move along with the drill string. It became clear that dividing signatures into groups based on causes of restrictions was not the best way to do it, but rather divide it into groups based on physical explanation such as fixed and moveable. From that point on causes of restrictions are related to one or two of the two main groups. The goal of distinguishing hook load signals from different causes of restrictions was reached to some extent. It was found that by recognizing if the restriction was fixed or moveable by looking at the hook load signal, 4 out of 5 causes of restrictions were distinguishable. This was possible because unstable wellbore was recognized by including both fixed and moveable restrictions and differential sticking was recognized by occurring at the beginning of a stand pulled.
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Branch, Perry L. "Development of real time non-intrusive load monitor for shipboard fluid systems." Thesis, (3 MB), 2008. http://handle.dtic.mil/100.2/ADA488243.
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Thesis (Degrees of Naval Engineer and M.S. in Engineering and Management)--Massachusetts Institute of Technology, June 2008."June 2008." Description based on title screen as viewed on August 26, 2009. DTIC Descriptor(s): Shipboard, Electrical Loads, Diagnostic Equipment, Monitoring, Prototypes, Reverse Osmosis, Graphical User Interface, Shipbuilding, Cost Effectiveness, Theses, Field Tests, Computer Aided Diagnosis, Maintenance Management. DTIC Identifier(s): Condition Based Maintenance Systems, Health Monitoring Systems, Reverse Osmosis Systems, Spectral Analysis, NILM (Nonintrusive Load Monitor), Fourier-Series Analysis Equations, Spectral Envelopes, Transient Electrical Behavior. Includes bibliographical references (p. 84-85). Also available in print.
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Branch, Perry L. (Perry Lamar). "Development of real time non-intrusive load monitor for shipboard fluid systems." Thesis, Massachusetts Institute of Technology, 2008. http://hdl.handle.net/1721.1/44846.
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Thesis (Nav. E.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering; and, (S.M.)--Massachusetts Institute of Technology, System Design and Management Program, 2008.Includes bibliographical references (p. 84-85).
Since the year 2000, the United States Navy has spent an average of half a billion dollars over the congressionally approved budget for shipbuilding. Additionally, most experts project that in order to meet the Chief of Naval Operation's goal of a 313 ship Navy, the annual ship building budget will have to increase by about two thirds. Exacerbating this problem is the rising cost of maintaining the current inventory of ships. The U.S. Navy has long used a requirements driven maintenance program to reduce the number of total system failures by conducting routine maintenance and inspections whether they are needed or not. In order to combat this problem the Navy will inevitably have to turn to a condition based maintenance system. The Non-Intrusive Load Monitor (NILM) is a system that can greatly enhance the ability to monitor the health of engineering systems while incurring a low acquisition cost and low technology risk. This research focuses on the development of a real time user interface for the current NILM architecture in order to provide useful system information to an operator. Additionally, this research has shown that the NILM can be used effectively and reliably, to monitor equipment health, recognize and indicate abnormal operating conditions and casualties and provide invaluable information for training operators, diagnosing problems and troubleshooting. The NILM is an inexpensive and promising platform for monitoring equipment and reducing maintenance costs.
by Perry L. Branch.
S.M.
Nav.E.
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Brajkovic, Jurica. "Evaluating investment in base load coal fired power plant using real options approach." Thesis, University of Southampton, 2010. https://eprints.soton.ac.uk/171553/.
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This thesis investigates the impact of uncertainty on investment in a coalfired power plant using a real options (RO) framework. It is organized in five chapters. In the first chapter I give an outline of the thesis. In Chapter 2 I review the background material. I describe the electricity sector in the pre- and post-liberalization periods and discuss the implication of the transition on investment in new generation capacity. Further, I analyze the mainstream approach to investment analysis used by the majority of electricity companies, the discounted cash flow (DCF) approach. Next, I describe an alternative approach for evaluating investments, RO. In Chapter 3 I perform an econometric analysis of dark spread prices. I select four different stochastic processes and fit them to the observed data. The goal is to find which of the four processes (arithmetic Brownian motion (ABM), Ornstein-Uhlenbeck (OU), Cox-Ingersoll-Ross (CIR) and the Schwartz one-factor process) can best describe the evolution of dark spread prices. The analysis shows that the CIR process is the most appropriate model to use to represent the evolution of dark spread prices. In Chapter 4 I evaluate an investment in a coal-fired power plant assuming the dark spread is the only source of uncertainty and using the stochastic processes for which I estimated parameters in Chapter 3. First I calculate the optimal investment threshold using a traditional budgeting approach based on the DCF principle. Following this, using the RO framework, I calculate the optimal investment threshold for the four stochastic processes. I conclude that one should use mean reverting process to model the investment decision but the choice of mean reverting process does not significantly affect the investment threshold values. In Chapter 5 I extend the analysis and model coal and electricity prices separately. Now the investment decision is affected by two factors: the price of electricity (output) and the price of coal (input). The goal of this chapter is to analyze whether this increase in complexity (going from a one-factor to a two-factor model) affects the result obtained in the previous chapter. Given the different dynamics of electricity and coal prices, I find that this approach enriches the investment analysis and gives additional insights. In particular, the higher the coal price, the greater the dark spread needs to be in order to undertake the investment. Finally, Chapter 6 concludes. The thesis contributes to the existing knowledge in several ways. RO have been applied to the electricity sector before, but this is the first time they have been applied to the evaluation of investment in a coal-fired power plant. Secondly, this is the first time that dark spread, electricity and coal prices are modeled for use in a RO analysis. Finally, the thesis provides a comparison of investment analysis for a coal-fired power plant using RO based on single and two state variables, which has not been carried out so far.
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Liljeroth, Henrik. "Measuring and Analysing Execution Time in an Automotive Real-Time Application." Thesis, Linköping University, Department of Computer and Information Science, 2009. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-51691.
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Autoliv has developed the Night Vision system, which is a safety system for use incars to improve the driver’s situational awareness during night conditions. It is areal-time system that is able to detect pedestrians in the traffic environment andissue warnings when there is a risk of collision. The timing behaviour of programsrunning on real-time systems is vital information when developing and optimisingboth hardware and software. As a part of further developing their Night Visionsystem, Autoliv wanted to examine detailed timing behaviour of a specific part ofthe Night Vision algorithm, namely the Tracking module, which tracks detectedpedestrians. Parallel to this, they also wanted a reliable method to obtain timingdata that would work for other parts of that system as well, or even other applications.
A preliminary study was conducted in order to determine the most suitable methodof obtaining the timing data desired. This resulted in a measurement-based approachusing software profiling, in which the Tracking module was measured usingvarious input data. The measurements were performed on simulated hardwareusing both a cycle accurate simulator and measurement tools from the systemCPU manufacturer, as well as tools implemented specifically to handle input andoutput data.
The measurements resulted in large amounts of data used to compile performancestatistics. Using different scenarios in the input data, we were able to obtain timingcharacteristics for several typical situations the system may encounter duringoperation. By manipulating the input data we were also able to observe generalbehaviour and achieve artificially high execution times, which serves as indicationson how the system responds to irregular and unexpected input data.
The method used for collecting timing information was well suited for this particularproject. It provided the possibility to analyse behavior in a better waythan other, more theoretical, approaches would have. The method is also easilyadaptable to other parts of the Night Vision system, or other systems, with onlyminor adjustments to measurement environment and tools.
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Ansari, Meisam. "REAL-TIME CONGESTION MANAGEMENT IN MODERN DISTRIBUTION SYSTEMS." OpenSIUC, 2021. https://opensiuc.lib.siu.edu/dissertations/1904.
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In this research, the problem of real-time congestion management in a modern distribution system with massive active elements such as electric vehicles (EVs), distributed energy resources (DERs), and demand response (DR) is investigated. A novel hierarchical operation and management framework is proposed that can take advantage of the demand side contribution to manage the real-time congestion. There are five main steps in this framework as 1) the aggregators send their demand to the microgrid operators (MGOs), 2) the MGOs send their demand to the distribution system operator (DSO), 3) the DSO detects the congestions and calls the engaged MGOs to reduce their demand, 4) the MGOs update the electricity price to motivate the aggregators to reduce the overall demand, and 5) the DSO dispatches the system according to the finalized demand. The proposed framework is validated on two modified IEEE unbalanced test systems. The results illustrate two congestion cases at t=8:45 am and t=9:30 am in the modified IEEE 13-bus test system, which needs 363kW and 286 kW load reductions, respectively, to be fully addressed. MG#1 and MG#2 are engaged to maintain the 363 kW reduction at t=8:45, and MG#3 and MG#4 are called to reduce their demands by 386 kW at t=9:30 am. The overall interactions can relieve the congested branches. The DSO’s calculations show three congestions at t=1 pm, t=3 pm, and t=9 pm on the IEEE 123-bus test system. These congestion cases can be alleviated by reducing 809 kW, 1177 kW, and 497 kW from the corresponding MGs at t=1 pm, t=3 pm, and t=9 pm, respectively. The second part of the simulation results demonstrates that the proposed real-time data estimator (RDE) can reduce the DSO’s miss-detected congestion cases due to the uncertain data. There are two miss-detected congestions in the IEEE 13-bus test system at t=1:15 pm and t=1:30 pm that can be filtered for t=1:15 pm and minored for t=1:30 pm using the RDE. The proposed RDE can also reduce the miss-detected congestions from 18 cases to four cases in the IEEE 123-bus test system. As a result, the RDE can minimize the extra costs due to the uncertain data. The overall results validate that the proposed framework can adaptively manage real-time congestions in distribution systems.
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Donati, Elena. "Extensometers for real-time detection of the elements' weight in an integrated security system." Master's thesis, Alma Mater Studiorum - Università di Bologna, 2018.
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Desouky, Azza Ahmed El. "Accurate fast weather dependent load forecast for optimal generation scheduling in real time application." Thesis, University of Bath, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.392211.
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Kane, Thomas John S. M. Massachusetts Institute of Technology. "The NILM Dashboard : watchstanding and real-time fault detection using Non-intrusive Load Monitoring." Thesis, Massachusetts Institute of Technology, 2019. https://hdl.handle.net/1721.1/122320.
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Thesis: S.M., Massachusetts Institute of Technology, Department of Mechanical Engineering, 2019Thesis: S.M. in Naval Architecture and Marine Engineering, Massachusetts Institute of Technology, Department of Mechanical Engineering, 2019
Cataloged from PDF version of thesis.
Includes bibliographical references (pages 119-122).
Non-intrusive Load Monitoring (NILM) measures power at a central point in an electrical network and disaggregates individual load schedules from the overall power stream. This thesis presents the NILM Dashboard, a data-analysis and user interface tool that provides real-time machinery monitoring and fault diagnostics using NILM data. The Dashboard was developed and deployed for use onboard US Coast Guard Cutters to act as an automatic watchstander and condition-based maintenance aid. The effectiveness of the system is demonstrated on power data collected from electrical panels in the ship's engine room. Case studies are used to evaluate the Dashboard's ability to detect fault conditions in electromechanical systems.
by Thomas John Kane.
S.M.
S.M. in Naval Architecture and Marine Engineering
S.M. Massachusetts Institute of Technology, Department of Mechanical Engineering
S.M.inNavalArchitectureandMarineEngineering Massachusetts Institute of Technology, Department of Mechanical Engineering
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Shadid, Ahmad Jamal. "Efficient load balancing techniques for Real-Time RTI-based large-scale distributed simulation systems." Thesis, University of Ottawa (Canada), 2008. http://hdl.handle.net/10393/28023.
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The Real-time extension of the High Level Architecture (HLA) is very essential and useful for many large-scale distributed simulation systems. Most previous attempts to design the Real-Time Run Time Infrastructure (RT-RTI) have enabled the usage of supported scheduling and prioritization services from underlying Real-time operating systems (RTOSs) augmented by communication QoS mechanisms. In this thesis, we wish to build on this functionality by proposing an algorithm that differentiates services processing within the RTI itself by incorporating resources' load balancing mechanisms with several scheduling and allocation policies. We focus our efforts on making the RTIs internal operations organized and well suited to the tasks and services it will be providing throughout the lifetime of HLA-compliant federations.
The load-balancing mechanisms incorporated within the proposed system have been designed to build an efficient management scheme of the available processing resources. The proposed design have been heavily verified and tested from all related perspectives under the constraints of the Discrete Event System Specification (DEVS), especially the (RT-DEVS). This formalized system design approach have propagated a totally-new RT-RTI design that solves most of the shortcomings demonstrated and shown by previous designs.
We have proved through our analytical performance evaluation and simulation experiments that our proposed real-time RTI framework exhibits a better performance in all terms, especially in the number of tasks served within deadlines when compared with existing RT-RTI frameworks. Our simulation results from the design model of the RT-DEVS formalism have verified the experimental results of the real implementation of the proposed RT-RTI design. In addition, these simulation models are capable of predicting key factors that may affect the performance of the RT-RTI core system, and could be used as guidance in searching for the optimal design.
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Nigrini, L. B., and G. D. Jordaan. "Short term load forecasting using neural networks." Journal for New Generation Sciences, Vol 11, Issue 3: Central University of Technology, Free State, Bloemfontein, 2013. http://hdl.handle.net/11462/646.
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Published ArticleSeveral forecasting models are available for research in predicting the shape of electric load curves. The development of Artificial Intelligence (AI), especially Artificial Neural Networks (ANN), can be applied to model short term load forecasting. Because of their input-output mapping ability, ANN's are well-suited for load forecasting applications. ANN's have been used extensively as time series predictors; these can include feed-forward networks that make use of a sliding window over the input data sequence. Using a combination of a time series and a neural network prediction method, the past events of the load data can be explored and used to train a neural network to predict the next load point. In this study, an investigation into the use of ANN's for short term load forecasting for Bloemfontein, Free State has been conducted with the MATLAB Neural Network Toolbox where ANN capabilities in load forecasting, with the use of only load history as input values, are demonstrated.
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Matsukura, Tadashi. "Significance of serial real-time PCR monitoring of EBV genome load in living donor liver transplantation." Kyoto University, 2004. http://hdl.handle.net/2433/147525.
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ALBUQUERQUE, Breno Moacir Farias de. "Aplicação da PCR em Tempo Real Para Detecção, Tipificaçãoe Carga Viral de Papilomavírus Bovino." Universidade Federal de Pernambuco, 2012. https://repositorio.ufpe.br/handle/123456789/18509.
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O Papilomavírus bovino(BPV) é o agente etiológico da papilomatosebovina. Esta apresenta lesões que normalmente são benignas e tendem a regredir, porém podem progredir a uma neoplasia. Muitas metodologias utilizadas para detecção de BPV se mostram inespecíficas e apresentam reações cruzadas com outros organismos relacionados. No entanto, a reação quantitativa em tempo real emcadeia da polimerase (qPCR) é uma ferramenta de destaque na detecção, tipificação e quantificação de nucleotídeos e vem sendo utilizada na clínica para avaliar carga viral. O objetivo do trabalho foi desenvolver um novo protocolo de detecção, tipificação e quantificação de BPV através daqPCR. Foram desenhados cinco pares de primers, que possuem como alvo uma região conservada do genoma viral (gene L1) de diferentes BPVs. A seletividade dos primers foi testada in vitroe DNA extraído de células MDBK não infectadas foram utilizados como controle negativo. A técnica de qPCR permitiu detectar, tipificar e quantificar material viral dos BPVs 1, 2, 4, 5 e 6. O limiar relativo da detecção foi de 4fg de DNA,emtorno de 30-40 cópias de DNA/μL. Dos cinco pares de primers produzidos, quatro apresentaram mesmo perfil térmico durante a qPCR (qPCRBPV2, 4, 5 e 6), permitindo em um único procedimento detectar e tipificar os quatro tipos virais. A distinção das amostrasfoi realizada através da análise de meltingque permitiu tipificá-las. Através da metodologia desenvolvida foi observado que em lesões cutâneas de bovinos infectados com BPV a carga viral não se mostrou inferior a 1000 cópias/μL, enquanto que a técnica permite quantificar até um limiar de 40 copias de DNA/μl. Este trabalho possui relevância para validação de qPCR como diagnóstico da papilomatose bovina e particular importância quando aplicado em estudos da infecção pelo BPV e no monitoramento por veterinários da eficácia das futuras vacinas.
Bovine papillomavirus (BPVs) is the etiologic agent of bovine papilomatose which is characterized by hyper proliferative lesions. Papillomas in cattle are typically benignandoften regress, but occasionally lesions can persist and progress to malignant neoplasia.The majority of current techniques for identification of BPV is unspecific andpossessescross-reactivity with closely related organisms.The Real-time quantitative polymerase chain reaction assay (qPCR) has become an exceptional tool for detection and quantification of oligonucleotides and has been utilized increasingly on viral load evaluation.Aiming to develop a new protocol for fast detection, typification and quantification of BPV in qPCR, we designed five pairs of Oligonucleotides for BPV1, 2, 4, 5 and 6 focusing on L1 gene. The qPCR primers sets were testedin vitroandMadin-Darby Bovine Kidney Cells (MDBK)DNA was also used as negative control.The Real-time qPCR assay provided an accurate detection and quantification for the BPVs 1, 2, 4, 5 and 6. The relative detection limit for the assays was 4fg or 30 to 40genome equivalents. Four primers pairs (qPCRBPV2, 4, 5 and 6) had the same annealing temperature and their products showed differences on meltingpoints analyses. Through the meltingpoint analysis, samples can be identified and discriminated as a screening and then samples can be run for viral load. In our study we tested the viral load in bovine cutaneous skin warts and observed infections with 1000 copies/μl at least. However, this assay could reach levels of 40copies/μL. In conclusion, this methodology has an important impact on the validation of qPCR as a BPV diagnosis. Its relevance is proved when applied to BPV infection studies and the monitoring of the efficacy of future BPV vaccinesby veterinarians.
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Massignan, Julio Augusto Druzina. "Estimação de demanda em tempo real para sistemas de distribuição radiais." Universidade de São Paulo, 2016. http://www.teses.usp.br/teses/disponiveis/18/18154/tde-12092016-111442/.
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Para implantação de diversas funções de controle e operação em tempo real em Sistemas de Distribuição (SDs), como, por exemplo, restabelecimento de energia, é necessário um procedimento para representar a carga em tempo real. Ou seja, uma metodologia que possibilite a estimação em tempo real das demandas dos transformadores de distribuição que em geral não são monitoradas de forma direta. Para esse fim propõe-se, neste trabalho, um Estimador de Demanda em Tempo Real (EDTR) baseado em: informações off-line (consumo mensal dos consumidores e curvas de carga típicas); um algoritmo computacionalmente eficiente para cálculo de fluxo de potência baseado na estrutura de dados denominada Representação Nó-Profundidade (RNP); e nas poucas medidas disponíveis em tempo real nos SDs. O EDTR proposto opera em dois estágios: (1) Estimação Off-line das Demandas; e (2) Refinamento em Tempo Real das Demandas, executados em instantes diferentes (um de maneira off-line e outro em tempo real), de forma a prover uma estimativa das demandas dos transformadores de distribuição. Considerando somente as informações off-line, o EDTR proposto permite a estimação das demandas dos transformadores de distribuição com uma medida da incerteza da estimativa. Através do processamento das medidas disponíveis em tempo real, via um algoritmo eficiente para cálculo de fluxo de potência, o EDTR proposto permite o refinamento das estimativas off-line. Neste trabalho serão apresentados resultados de diversas simulações computacionais demonstrando a eficiência do EDTR proposto. Alguns parâmetros são avaliados quanto à influência nas estimativas do EDTR proposto, como a presença de erros grosseiros nas medidas disponíveis em tempo real e alimentadores somente com medidas de magnitude de corrente. Além disto, destaca-se a influência da qualidade das estimativas iniciais obtidas pelo Estágio (1), e a importância das hipóteses estatísticas utilizadas nesse estágio para o processo de estimação. Apresenta-se, ainda, a aplicação do EDTR proposto em um SD real brasileiro. Um teste de validação foi realizado através de uma campanha de medição em um alimentador real, que consistiu na instalação de medidores de demanda em três transformadores de distribuição para aferir a qualidade das estimativas obtidas pelo EDTR proposto. Finalmente, o EDTR proposto é aplicado em um SD real de larga escala, para aferir o desempenho computacional da metodologia implantada e as dificuldades de implantação. Vale ressaltar que sua implantação é condizente com ferramentas consolidadas nos Centros de Operação da Distribuição, como o uso do processo de agregação de cargas e o cálculo de fluxo de potência, e poucas rotinas precisam ser adicionadas para integração do EDTR.Several real time control and operation applications for Distribution Systems (DS), such as, service restoration, require a procedure for real time load modeling. That is, a methodology for real time estimation of the distribution transformers loading which are generally not monitored. For this purpose, in this dissertation, a Real Time Load Estimator (RTLE) is proposed based on: off-line information (monthly consumption and typical load curves); a computationally efficient algorithm for power flow calculation based on the data structure called Node-Depth Encoding; and on the few available real time measurements on the distribution system. The proposed RTLE operates in two stages: (1) Off-line Load Estimation and (2) Real Time Load Refinement, performed in different moments (one off-line and the other in real time), providing the distribution transformers load estimates. Using only the offline information, the proposed RTLE allows the estimation of the loads of the distribution transformers with a measure of uncertainty. By processing the available real time measurements, using an efficient power flow calculation algorithm, the proposed RTLE refines these off-line estimates. This dissertation presents several simulations showing the efficiency of the proposed RTLE. Some parameters are evaluated and their influence on the RTLE load estimates, such as gross errors in the available real time measurements and feeders with only current magnitude measurements. Besides, it is emphasized the influence of the initial load estimates obtained from Stage (1), and the importance of the statistical hypothesis used in this stage in the load estimation process. Also, this work presents the application of the proposed RTLE in a real Brazilian DS. A validation test was performed through in-field verification in a real distribution feeder, which was executed via load meters installation in three distribution transformers to evaluate the quality of the load estimates provided by the RTLE. Finally, the proposed RTLE was tested in a real large scale DS to evaluate its computational performance and the difficult level of its implementation. It is noteworthy that its implementation is straightforward with other Distribution Operation Center tools, such as load aggregation and load flow calculation, and few routines must be added for integrating the RTLE.
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Johnstone, Nicola. "How load bearing can influence cognitive performance : investigated using mobile electroencephalography (EEG) recordings in real-life settings." Thesis, University of Surrey, 2018. http://epubs.surrey.ac.uk/845540/.
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Physical load carriage is an essential duty for military personnel. Physical load carriage induces psychological and physiological effects, but the influence of load carriage on brain activity and subsequent cognitive performance is largely unknown. This due to difficulties in disentangling the subtle behavioural effects under load carriage in complex occupational settings. To investigate how load carriage influences cognition directly, continuous measures of brain activity must be obtained in realistic settings. Mobile EEG is ideally suited to this task. Across four studies, mobile EEG recordings were used to assess to what extent load carriage influences attention. Four objectives were defined. 1. To evaluate mobile EEG as a viable tool in real-life settings (studies 1-4). 2. Investigate the influence of walking on attentional processes (study 1, selective attention; study 2, change detection). 3. Establish the impact of load carriage on brain activity during walking (study 3). 4. Assess the interaction of load carriage on attentional processes during walking (study 4). Interpretations of resulting data were conceptualised in the proposed dynamic resource allocation model of cognitive performance. Successful application of Mobile EEG was evident and found signal quality comparable to EEG recorded in a laboratory. Moreover, walking influenced attentional processes in an indirect way, moderated by the environment and concurrent perceptual load. While a steady state neural index of walking was recorded in a natural setting, this was invulnerable to load carriage effects. Further, and most pressing for this thesis, load carriage had little influence on neural indices of attention. Despite this, there is some indication from the data that fatigue plays a prominent role in the interaction of load carriage and cognitive function, and further exploring the neural underpinnings of motivation may prove fruitful in future studies of load carriage and cognitive function.
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Mengistu, Meron Mulatu. "Study of Solar Cooling Alternatives for Residential Houses in Bahir dar city :." Thesis, KTH, Energiteknik, 2011. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-63419.
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The energy consumption rate of non-OECD countries rises about 2.3 percent per year as compared to the energy consumption rate of OECD countries which is 0.6 percent. If developing countries use energy efficient technology and integrate renewable energy systems in the new building their carbon dioxide emission rate reduces by 25 to 44 percent. However, even now, renewable energy integrated buildings are hardly considered while constructing them. This thesis work focuses on the study of solar cooling system options for residential house in Bahir Dar city. To meet the demand of housing in the city, different type of apartments and villa houses are under construction. Case study was made focusing on two types of residential houses (condominium apartment and Impact Real-estate Villa house) to determine the cooling load and to select cooling system. Simulation results of IDA ICE software show that the average operative temperatures and cooling loads for condominium apartment and Real-estate Vila are 31.8oC and 30.7oC, 5.53 kW and 5.73 kW respectively. Most of the residences are not satisfied at this operating temperature. There are different types of solar cooling systems. Solar Sorption cooling systems are commonly used which can also be classified in to absorption, adsorption and desiccant cooling systems. Solar adsorption cooling systems are easy to manufacture locally as compared to solar absorption cooling systems. They do not have moving parts. Some of the working medium pairs used in adsorption cooling system are: Activated Carbon/Ammonia, Silica gel/ water, Zeolite/water. Adsorption chillier with Silica gel/ water as a working pair was selected since it can operate at regeneration/desorption temperature as low as 45oC coming from flat plate collectors. At 75oC regeneration temperature, the system delivers 9oC chilled water temperature. The selected solar adsorption chiller has been compared with kerosene based adsorption cooling system using HOMER software. In this project, the optimization was limited on cost comparison between the two energy sources. The solar based cooling system has lower working cost. From cooling load simulation result direct solar irradiation is the highest source of cooling load for both houses. This gives an opportunity for passive solar cooling technology.
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De, Lange Nico Louis. "Research into real-time energy management on old gold mines / N.L. de Lange." Thesis, North-West University, 2006. http://hdl.handle.net/10394/1345.
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Wilson, Pat H. "Does real-time visual feedback improve pitch accuracy in singing?" University of Sydney, 2007. http://hdl.handle.net/2123/1526.
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Master of Applied ScienceThe aim of this investigation was to investigate the effects of computer-based visual feedback in the teaching of singing. Pitch accuracy, a readily-measured parameter of the singing voice, was used in this study to gauge changes in singing for groups with and without visual feedback. The study investigated whether the style of feedback affects the amount of learning achieved, and whether the provision of concurrent visual feedback hampers the simultaneous performance of the singing task. The investigation used a baseline–intervention–post-test between-groups design. Participants of all skill levels were randomly assigned to a control group or one of two experimental groups – with all participants given one hour of singing training. At intervention, the two experimental groups were offered one of two different displays of real-time visual feedback on their vocal pitch accuracy, while control participants had a non-interactive display. All sessions were recorded, and the vocal exercise patterns performed at baseline, intervention and post-test phases were acoustically analysed for pitch accuracy. Questionnaires assessed both general health and the amount of singing and music training of all participants; people in the two experimental groups were also given a further questionnaire about the visual feedback. The results indicate that visual feedback improves pitch accuracy in singing. Cognitive load related to the decoding of visual information was a factor at intervention. At post-test, the two groups who had used real-time visual feedback demonstrated marked improvement on their initial pitch accuracy. There was no significant difference between the results of participants from the two experimental groups, although the participants with some background in singing training showed greater improvement using a simpler visual feedback design. The findings suggest that a hybrid approach integrating standard singing teaching practices with real-time visual feedback of aspects of the singing voice may improve learning.
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Gedda, Emil, and Anders Eriksson. "Practical analysis of the Precision Time Protocol under different types of system load." Thesis, KTH, Skolan för datavetenskap och kommunikation (CSC), 2017. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-208493.
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The existence of distributed real-time systems calls for protocols for high accuracy time synchronization between devices. One such protocol, the Precision Time Protocol (PTP) reaches sub microsecond synchronization precision. PTP can be implemented both in hardware and software. This study aimed to analyze how system stress could affect the accuracy and precision of software implemented PTP between two devices. This was done using two Intel Galileo Generation 2 running Linux systems. Software was used to simulate CPU, I/O, network, and OS usage. Data was extracted from software logs and summarized in charts and then analyzed. The results showed that PTP synchronization accuracy and precision does suffer under certain types of system load, most notably under heavy I/O load. However the results might not be applicable to real-world scenario due to limitations in hardware and the synthetic stress tests do not correspond to real-world usage. Further research is required to analyze why and how different types of system load affects PTPs accuracy and precision.Förekomsten av distribuerade realtidssystem kräver protokoll för noggrann tidssynkronisering mellan enheter. Ett sådant protokoll, Precision Time Protocol (PTP), kan uppnå en precision på under mikrosekunden under synkronisering. PTP kan implementeras i både hårdvara och mjukvara. Den här rapporten fokuserar på att analysera hur systembelastning kan påverka precision och noggrannheten hos mjukvaruimplementerad PTP mellan två enheter. Testen utfördes på två stycken Intel Galileo Generation 2 kö- randes Linux. Mjukvara användes sedan för att simulera belastning på olika system såsom CPU, I/O, nätverk och på operativsystemet. Data extraherades ifrån loggar från mjukvaran, vilken sammanfattades i grafer för att sedan analyseras. Resultaten visade att precisionen och noggrannheten hos PTP försämras under vissa typer av systembelastningar, mest märkbart under tung I/O belastning. Resultaten är dock potentiellt inte applicerbara på verklighetscenarion på grund av begränsingar i hårdvaran samt att syntetiska stresstest inte motsvarar normal belastning. Ytterligare forskning krävs för att analysera hur och varför olika typer av systembelastning påverkar PTPs precision och noggrannhet.
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Cannon, Jordan. "Statistical analysis and algorithms for online change detection in real-time psychophysiological data." Thesis, University of Iowa, 2009. https://ir.uiowa.edu/etd/342.
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Modern systems produce a great amount of information and cues from which human operators must take action. On one hand, these complex systems can place a high demand on an operator's cognitive load, potentially overwhelming them and causing poor performance. On the other hand, some systems utilize extensive automation to accommodate their complexity; this can cause an operator to become complacent and inattentive, which again leads to deteriorated performance (Wilson, Russell, 2003a; Wilson, Russell, 2003b). An ideal human-machine interface would be one that optimizes the functional state of the operator, preventing overload while not permitting complacency, thus resulting in improved system performance.
An operator's functional state (OFS) is the momentary ability of an operator to meet task demands with their cognitive resources. A high OFS indicates that an operator is vigilant and aware, with ample cognitive resources to achieve satisfactory performance. A low OFS, however, indicates a non-optimal cognitive load, either too much or too little, resulting in sub-par system performance (Wilson, Russell, 1999).
With the ability to measure and detect changes in OFS in real-time, a closed-loop system between the operator and machine could optimize OFS through the dynamic allocation of tasks. For instance, if the system detects the operator is in cognitive overload, it can automate certain tasks allowing them to better focus on salient information. Conversely, if the system detects under-vigilance, it can allocate tasks back to the manual control of the operator. In essence, this system operates to "dynamically match task demands to [an] operator's momentary cognitive state", thereby achieving optimal OFS (Wilson, Russell, 2007).
This concept is termed adaptive aiding and has been the subject of much research, with recent emphasis on accurately assessing OFS in real-time. OFS is commonly measured indirectly, like using overt performance metrics on tasks; if performance is declining, a low OFS is assumed. Another indirect measure is the subjective estimate of mental workload, where an operator narrates his/her perceived functional state while performing tasks (Wilson, Russell, 2007). Unfortunately, indirect measures of OFS are often infeasible in operational settings; performance metrics are difficult to construct for highly-automated complex systems, and subjective workload estimates are often inaccurate and intrusive (Wilson, Russell, 2007; Prinzel et al., 2000; Smith et al., 2001).
OFS can be more directly measured via psychophysiological signals such as electroencephalogram (EEG) and electrooculography (EOG). Current research has demonstrated these signals' ability to respond to changing cognitive load and to measure OFS (Wilson, Fisher, 1991; Wilson, Fisher, 1995; Gevins et al., 1997; Gevins et al., 1998; Byrne, Parasuraman, 1996). Moreover, psychophysiological signals are continuously available and can be obtained in a non-intrusive manner, pre-requisite for their use in operational environments.
The objective of this study is to advance schemes which detect change in OFS by monitoring psychophysiological signals in real-time. Reviews on similar methods can be found in, e.g., Wilson and Russell (2003a) and Wilson and Russell (2007). Many of these methods employ pattern recognition to classify mental workload into one of several discrete categories. For instance, given an experiment with easy, medium and hard tasks, and assuming the tasks induce varying degrees of mental workload on a subject, these methods classify which task is being performed for each epoch of psychophysiological data. The most common classifiers are artificial neural networks (ANN) and multivariate statistical techniques such as stepwise discriminant analysis (SWDA). ANNs have proved especially effective at classifying OFS as they account for the non-linear and higher order relationships often present in EEG/EOG data; they routinely achieve classification accuracy greater than 80%.
However, the discrete output of these classification schemes is not conducive to real-time change detection. They accurately classify OFS, but they do not indicate when OFS has changed; the change points remain ambiguous and left to subjective interpretation. Thus, the present study introduces several online algorithms which objectively determine change in OFS via real-time psychophysiological signals.
The following chapters describe the dataset evaluated, discuss the statistical properties of psychophysiological signals, and detail various algorithms which utilize these signals to detect real-time changes in OFS. The results of the algorithms are presented along with a discussion. Finally, the study is concluded with a comparison of each method and recommendations for future application.
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Avelino, Luana Locatelli. "Estimação de demanda trifásica em tempo real para sistemas de distribuição radiais." Universidade de São Paulo, 2018. http://www.teses.usp.br/teses/disponiveis/18/18154/tde-03082018-095459/.
Full textAbstract:
Os estimadores de estado são ferramentas primordiais para monitoramento em tempo real dos sistemas elétricos de potência, principalmente por permitir a execução de funções básicas relacionadas à segurança destes sistemas. No entanto, nos sistemas de distribuição, por possuírem uma série de particularidades e pelo reduzido número de medidas disponíveis em tempo real, os estimadores de estado convencionais desenvolvidos para sistemas de transmissão não são capazes de oferecer boas estimativas para determinação do estado da rede. Neste contexto, o Estimador de Demanda Trifásico em Tempo Real (EDTTR) desenvolvido neste trabalho, fundamentado nos métodos de estimação de demanda, tem como foco principal proporcionar maior precisão ao monitoramento da rede primária de alimentadores de distribuição radiais, ao contemplar as características intrínsecas do modelo da rede. O EDTTR consiste em dois estágios principais: o primeiro é caracterizado pela estimação off-line das demandas dos transformadores de distribuição por meio do processo de agregação de cargas, considerando informações dos consumidores. O segundo estágio é realizado em tempo real, utiliza um algoritmo eficiente de Varredura Direta Inversa para a solução do fluxo de carga, auxiliado da estrutura de dados chamada Representação Nó-Profundidade para armazenar a topologia da rede. Neste estágio também ocorre o ajuste recursivo das estimativas obtidas no Estágio 1 com base nas poucas medidas disponíveis em tempo real. No intuito de melhorar a representação da rede primária, o EDTTR desenvolvido possibilita o tratamento dos transformadores de distribuição conectados em Delta-Yn. Esta proposição permite uma caracterização do modelo das cargas mais fiel, sendo capaz de superar problemas de convergência, além de contribuir para a qualidade do processo de estimação. Desenvolveu-se, também, uma plataforma de testes que permite a análise do impacto de fatores que deterioram o processo de estimação de demandas, como: a má classificação dos consumidores, o comportamento dos mesmos, que na prática não é exatamente igual ao da classe de carga a ele atribuída, a possibilidade de perdas não técnicas, erros grosseiros e falhas de comunicação nos medidores do alimentador. O EDTTR foi validado em simulações utilizando um alimentador real da cidade de Ribeirão Preto com base em dados fornecidos pela distribuidora CPFL Paulista.The state estimators are primordial tools for electric power systems real time monitoring. These tools allow the execution of basic functions related to systems security. However, conventional state estimators, developed for transmission systems, can not provide good estimates to the state of distribution systems. This is due to two main factors, the few real time measures available in distribution systems, and the intrinsic complexity of them. In this context, the developed Real Time Three-Phase Load Estimator (EDTTR), based on load estimation methods, aims to provide better estimates to the monitoring of the primary network of radial distribution feeders, considering all the intrinsic sets of the system model. The EDTTR consists of two main stages: the first is characterized by the off-line load estimation of the distribution transformers by a load aggregation process, which uses consumers information as input data. In the second stage the off-line estimates obtained in Stage 1 are recursively refined in real-time basing on the available measurements. This procedure is executed by a computationally efficient backward/forward sweep load flow algorithm based on the data structure called Node-depth Encoding. The developed EDTTR allows the treatment of distribution transformers connected in Delta-Yn improving the primary network representation. A test platform is also developed that enables the analysis of the impact of factors that deteriorate the demand estimation process, such as the poor classification of consumers, the poor representativeness of the load class for certain consumers, the possibility of non-technical losses, and of gross errors and communication faults in the feeder meters. The developed EDTTR performance was tested in simulations that uses a real feeder from Ribeirão Preto city, based on system data provided by CPFL Paulista utility.
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Neusser, Lukas. "Combinação seletiva de métodos para previsão de demanda a curtíssimo prazo em tempo real." Universidade Federal de Santa Maria, 2015. http://repositorio.ufsm.br/handle/1/3692.
Full textAbstract:
In transforming the current electricity network, in a so called smart grid, demand forecasting
is relevant to processes such as demand management, demand response, distributed
generation, among others. For consumers, the replacement of electromechanical meters by
electronic meters, enables real-time access to measurement data, providing this data for demand
forecasting.
The present work focuses on consumers with different profiles, commercial, industrial
and institutional, connected to the the distribution network in medium-voltage and loads ranging
between a few tens of kilowatts and two megawatts. For these consumers, very short-term
demand forecasting (up to 2 hours) will be an important tool for decision making in a dynamic
environment, with time-variable energy prices, demand-side management and eventually own
generation.
With the application of demand forecasting methods to various consumers with different
profiles, it is shown that the forecasting methods with better accuracy (lower average error) are
variable from consumer to consumer. For one consumer individually, the method with better
accuracy is also variable, depending on the hour of the day.
Combination of several demand forecasting methods results in similar or better performance
compared to using only a single method. A method of selective combination is proposed,
in order to eliminate the risk of choosing a unique method, which results are unpredictable. The
results of the application of the proposed combination method, on several consumers with different
characteristics, demonstrate that selective combination improves the quality of the forecast.No processo de transformação das redes de energia elétrica atuais, em redes elétricas inteligentes (smart grid), a previsão de demanda é relevante para processos como o gerenciamento da demanda, resposta a demanda, geração distribuída, entre outros. Para os consumidores, a substituição de medidores eletromecânicos, por medidores eletrônicos, possibilita o acesso em tempo real aos dados da medição, disponibilizando estes dados para a previsão de demanda. O presente trabalho focaliza em consumidores de diferentes perfis, comerciais, industriais e institucionais, ligados à rede de distribuição em média tensão, com demandas situadas em uma faixa de algumas dezenas de quilowatts até dois megawatts. Para estes consumidores, uma previsão de demanda para curtíssimo prazo (até 2 horas) será uma importante ferramenta na tomada de decisão em um ambiente dinâmico, com tarifas variáveis no tempo, gerenciamento pelo lado da demanda e eventual geração própria. A partir da aplicação de métodos de previsão de demanda em consumidores de variados perfis de carga, é demonstrado que os métodos de previsão com melhor precisão (menor erro médio) variam de consumidor para consumidor. Para um mesmo consumidor o método de melhor precisão também é variável, sendo dependente da hora do dia. Uma combinação de diversos métodos de previsão de demanda resulta em performance similar ou superior se comparado ao uso de apenas um método individual. Propõe-se um método de combinação seletiva, com o objetivo de eliminar o risco da escolha de um único método, cujos resultados são imprevisíveis. Os resultados da aplicação do método de combinação proposto, em diversos consumidores de características diferentes, demonstram que a combinação seletiva representa uma melhora na qualidade da previsão.
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McCoy, Allen H. "Flight testing and real-time system identification analysis of a UH-60A Black Hawk Helicopter with an instrumented external sling load." Monterey, Calif. : Springfield, Va. : Naval Postgraduate School ; Available from National Technical Information Service, 1997. http://handle.dtic.mil/100.2/ADA343449.
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Thesis (M.S. in Aeronautical Engineering) Naval Postgraduate School, December 1997."December 1997." Thesis advisor(s): E. Roberts Wood, Mark B. Tischler. Includes bibliographical references (p. 87-88). Also available online.
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Barletta, Francesca, Theresa J. Ochoa, Erik H. Mercado, Joaquim Ruiz, Lucie Ecker, Giovanni Lopez, Monica Mispireta, Ana I. Gil, Claudio F. Lanata, and Thomas G. Cleary. "Quantitative real-time polymerase chain reaction for enteropathogenic Escherichia coli: a tool for investigation of asymptomatic versus symptomatic infections." Oxford University Press, 2015. http://hdl.handle.net/10757/556075.
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theresa.j.ochoa@uth.tmc.eduArticle
BACKGROUND: Enteropathogenic Escherichia coli (EPEC) strains are pediatric pathogens commonly isolated from both healthy and sick children with diarrhea in areas of endemicity. The aim of this study was to compare the bacterial load of EPEC isolated from stool samples from children with and without diarrhea to determine whether bacterial load might be a useful tool for further study of this phenomenon. METHODS: EPEC was detected by polymerase chain reaction (PCR) of colonies isolated on MacConkey plates from 53 diarrheal and 90 healthy children aged <2 years. DNA was isolated from stool samples by cetyltrimethylammonium bromide extraction. To standardize quantification by quantitative real-time PCR (qRT-PCR), the correlation between fluorescence threshold cycle and copy number of the intimin gene of EPEC E2348/69 was determined. RESULTS: The detection limit of qRT-PCR was 5 bacteria/mg stool. The geometric mean load in diarrhea was 299 bacteria/mg (95% confidence interval [CI], 77-1164 bacteria/mg), compared with 29 bacteria/mg (95% CI, 10-87 bacteria/mg) in control subjects (P = .016). Bacterial load was significantly higher in children with diarrhea than in control subjects among children <12 months of age (178 vs 5 bacteria/mg; P = .006) and among children with EPEC as the sole pathogen (463 vs 24 bacteria/mg; P = .006). CONCLUSIONS: EPEC load measured by qRT-PCR is higher in diarrheal than in healthy children. qRT-PCR may be useful to study the relationship between disease and colonization in settings of endemicity.
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Zhang, Duo. "Evaluation of the effectiveness of two irrigation systems in reducing bacterial load in root canals in vitro by quantitative real-time polymerase chain reaction." Thesis, University of British Columbia, 2017. http://hdl.handle.net/2429/62461.
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Introduction: Irrigation is regarded as having a key role in one of the main goals of endodontic treatment, the killing and removing bacteria from the infected root canal. Different methods and techniques have been used to deliver irrigating solutions and to facilitate the effectiveness of irrigation. The purpose of this in vitro study was, by using quantitative real-time PCR to determine the effectiveness of two irrigation and cleaning systems in removing multispecies oral biofilms from root canals.
Methods: Fifteen extracted human molars were instrumented to size #15/.02 and then cleaned with the GentleWave System (GW; Sonendo Inc, Laguna Hills, CA). The teeth were autoclaved to provide the same sterile baseline. The molars were filled with mixed plaque suspended in brain-heart infusion broth and centrifuged to help the bacteria spread all over the root canal system. After two weeks of incubation, samples were divided randomly into two treatment groups and instrumented into size #15/.04 (GW) and #35/.04 (PiezoFlow group) under needle irrigation with sterile water. The teeth were then cleaned either with GentleWave System or ProUltra PiezoFlow Active Ultrasonic System (Dentsply Tulsa, Tulsa, OK) using 3% NaOCl, 8% EDTA and sterile water as irrigants. Root canals were sampled with paper points before and after instrumentation (S1, S2) and after GW or PF cleaning (S3). Quantitative real-time PCR was performed, the presence of microorganism in the samples was determined by using universal bacterial, a genus specific and species-specific primers. Statistical analysis was performed using the Mann-Whitney U test with the significance level set at P < 0.05.
Result: A highly significant bacterial reduction was recorded for both groups (P < 0.001). GW group showed a significantly higher reduction than the PF group of total microbes (P < 0.01), Enterococcus faecalis (P < 0.01) and Streptococcus species (P < 0.05). GW resulted predictably in high reduction of over 99% of the microbes in every canal, whereas in the PF group the reduction of microbes varied between 87% and 99.99%.
Conclusion: While both systems demonstrated effective reduction of intracanal bacteria, a high level of over 99% was more predictably achieved in the GW group.Dentistry, Faculty of
Graduate
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Foulard, Stéphane. "Online and real-time load monitoring for remaining service life prediction of automotive transmissions : damage level estimation of transmission components based on a torque acquisition." Thesis, Ecully, Ecole centrale de Lyon, 2015. http://www.theses.fr/2015ECDL0012.
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Ce travail de recherche propose le développement et la validation d’une méthode de prédiction in-situ et temps réel de la durée de vie utile restante des engrenages de boîtes de vitesses automobiles de série. Cette méthode est destinée à être implantée dans des unités de commande électronique standards. En s’attachant tout particulièrement à proposer une solution simple, fiable, rentable et facilement transposable pour tout type de configuration, le système se base sur la combinaison d’une méthode d’acquisition des couples agissants sur la boîte de vitesses et d’une estimation continue des niveaux d’endommagement des engrenages. Un état de l’art et les fondamentaux théoriques d’une estimation de l’endommagement par une approche de contrainte nominale et une accumulation linéaire des endommagements partiels sont abordés dans un premier temps. La structure globale de l’algorithme de calcul de l’endommagement est ensuite étudiée et l’approche méthodologique adoptée pour sa mise au point expliquée. Cette dernière repose en grande partie sur un modèle complet de véhicule valide par des essais sur route et des mesures, où une attention toute particulière est portée à la représentation des changements de rapport et de la dynamique de la boîte de vitesses. Deux types de boite de vitesses sont alors considérées, une boite manuelle standard et une boite à double embrayage, et une spécification technique pour la configuration de l’algorithme ainsi qu’une analyse des besoins pour la méthode d’acquisition des couples sont formulées. En se basant sur ces études, un observateur d’état capable de reconstruire le couple agissant sur les disques d’embrayage ainsi que le couple en sortie de boite est développé et valide. Finalement, une synthèse de la méthode complète et de l’algorithme final est adressée, et les avantages économiques et écologiques liés à l’introduction de cette méthode pour des mesures de conception légère des boîtes de vitesses automobiles sont abordés et évaluésThis research work proposes the development and the validation of an online and real-time method to predict the remaining service life of the gearwheels of automotive transmissions, with the aim of implementing it on standard control units of series-production vehicles. By focusing on the proposition of a simple, reliable and easy-to-implement solution, the system relies on the combination of an acquisition method of the torques acting in the transmission and a continuous estimation of the damage levels of the gearwheels. Firstly, a state of the art and the theoretical basics are presented concerning a damage estimation based on a nominal stress concept and a linear damage accumulation. The global structure of the damage estimation algorithm is then analyzed and the methodological approach adopted for its development is explained. This is based in principal on a drivetrain model, validated with tests and measurements, where a particular attention is paid to the representation of the gear shifts and the transmission dynamics. Two types of transmissions are considered, namely a standard manual transmission and a dual clutch transmission mounted in series-production cars. Respectively a requirement analysis for the configuration of the algorithm as well as a requirement specification for the torque acquisition method are performed. On this basis, a state observer is developed and validated, which is able to reconstruct the clutch torque and the transmission output torque. Finally, a synthesis of the complete method and the final version of the algorithm are addressed, and the economic and ecological advantages of the introduction of the method in the context of lightweight design measures are discussed and evaluated
Kurzfassung Diese Dissertation beschreibt die Entwicklung einer Online- und Echtzeit-Methode zur Vorhersage der restlichen Lebensdauer von den Zahnradern eines Kraftfahrzeuggetriebes. Diese Methode ist fur eine Implementierung auf Standard-Steuergeraten vorgesehen. Durch die Fokussierung auf eine einfache, zuverlassige und leicht zu implementierende Losung beruht die Methode auf der Kombination aus einer Drehmomenterfassungsmethode und einer kontinuierlichen Vorhersage des Schadigungsniveaus der Zahnrader. Zuerst werden der Stand der Technik und die theoretischen Grundlagen von Schadigungsberechnungen basierend auf dem Nennspannungskonzept und einer linearen Schadensakkumulation dargestellt. Danach wird die globale Struktur des Schadigungsberechnungsalgorithmus gezeigt und die fur die Entwicklung ausgewahlte methodische Vorgehensweise erlautert. Diese bezieht sich grundsatzlich auf ein durch Testfahrten und Messungen verifiziertes Antriebsstrangmodell, welches besonders die Schaltungen und die Dynamik des Getriebes berucksichtigt. Ein Serien-Handschaltgetriebe und ein Serien-Doppelkupplungsgetriebe werden betrachtet. Fur diese zwei Getriebetypen werden eine Anforderungsanalyse zur Konfiguration des Algorithmus sowie eine Anforderungsspezifikation fur die Drehmomenterfassungsmethode durchgefuhrt. Auf Basis dieser Untersuchungen wird dann ein Zustandsbeobachter zur Rekonstruktion des Kupplungs- und Getriebeausgangsdrehmoments entwickelt und validiert. Infolgedessen werden eine Synthese der kompletten Methode und die Endversion des Algorithmus vorgestellt. Abschliesend werden die Wirtschaftlichkeit sowie die okologischen Vorteile in Bezug auf die Einfuhrung der Lebensdauermonitoringmethode im Rahmen von Leichtbaumasnahmen diskutiert und bewertet
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Montanheiro, Patricia Aparecida. "Quantificação de carga proviral do vírus linfotrópico de células T humanas tipo 1 (HTLV-1) e marcadores imunológicos em indivíduos portadores e pacientes com TSP/HAM." Universidade de São Paulo, 2007. http://www.teses.usp.br/teses/disponiveis/42/42132/tde-31012008-122000/.
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Na cidade de São Paulo, cerca de 50 mil pessoas são portadoras do HTLV-1. O HTLV-1 é o agente causador da paraparesia espástica tropical/mielopatia associada com o HTLV-1 (TSP/HAM) e os mecanismos desta patogênese são obscuros. A TSP/HAM é considerada uma doença imuno-mediada e algumas citocinas, podem estar associadas, com a desmielinização da membrana de mielina da coluna espinhal, provavelmente, estimuladas pela presença de antígenos virais. A PCR em tempo real é uma técnica utilizada para a detecção de citocinas e apresenta maior sensibilidade na expressão de mRNA e carga proviral do HTLV-1. Objetivos: Detecção de citocinas pelas técnicas sorológicas e de biologia molecular (PCR em tempo real) que auxiliaram no aconselhamento e avaliação de pacientes infectados pelo HTLV-1, além de uma avaliação da carga proviral dos pacientes com infecção pelo HTLV-1. Casuística: Grupo I: indivíduos soronegativos para HCV, HIV-1 e HTLV-1 (Controle); Grupo II: pacientes HTLV-1 assintomáticos; Grupo III: pacientes com TSP/HAM. Observamos que o INF-g apresenta-se alterado na infecção HTLV-1, sendo um dos fatores mais importantes da evolução para TSP/HAM, ambos os ensaios apresentaram o mesmo resultado. A carga proviral pode ser um marcador de progressão para a TSP/HAM. A interação complexa existente entre o HTLV-1 e as células responsáveis pela liberação de citocinas e b-quimiocinas pró-inflamatórias, assim como elementos de ativação celular, resultam na ativação imunológica. Isto, lentamente, pode levar ao processo inflamatório crônico que atua diretamente no micro ambiente neuronal e/ou membrana de mielina da coluna espinhal, em alguns portadores de HTLV-1. Com a persistente ativação do sistema imunológico, este processo provocará destruição das células gliais e dos neurônios, dando início ao processo de desmielinização.In São Paulo, about 50 thousand people are HTLV-1 carriers. HTLV-1 is the agent that causes tropical spastic paraparesis and HTLV-1 associated myelopathy (TSP/HAM) despite the mechanisms of this disease are still unclear. TSP/HAM is considered an immune mediated illness and some cytokines might be involved with axonal damage and demyelination, most pronounced in the midthoracic spinal cord, probably stimulated by the presence of viral antigens. Real Time PCR is a used technique to cytokine detection and shows higher sensitivity on mRNA expression and HTLV-1 proviral load. Objectives: cytokine detection through molecular biology (Real time PCR) and serologic techniques that helped on advising and assessment of HTLV-1 infected patients and also on their proviral load. Casuistic: Group I: seronegative individuals for HCV, HIV-1 and HTLV-1 (control); Group II: asymptomatic HTLV-1 infected patients; Group III: TSP/HAM patients. We\'ve been observed that g-interferon presents changing on the HTLV-1 infection, being one of the most important factors to TSP/HAM progression, both essays showed the same results. The proviral load may be an important marker for TSP/HAM development.
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Persson, Magnus. "Adaptive Middleware for Self-Configurable Embedded Real-Time Systems : Experiences from the DySCAS Project and Remaining Challenges." Licentiate thesis, KTH, Machine Design (Div.), 2009. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-11608.
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Development of software for embedded real-time systems poses severalchallenges. Hard and soft constraints on timing, and usually considerableresource limitations, put important constraints on the development. Thetraditional way of coping with these issues is to produce a fully static design,i.e. one that is fully fixed already during design time.Current trends in the area of embedded systems, including the emergingopenness in these types of systems, are providing new challenges for theirdesigners – e.g. integration of new software during runtime, software upgradeor run-time adaptation of application behavior to facilitate better performancecombined with more ecient resource usage. One way to reach these goals is tobuild self-configurable systems, i.e. systems that can resolve such issues withouthuman intervention. Such mechanisms may be used to promote increasedsystem openness.This thesis covers some of the challenges involved in that development.An overview of the current situation is given, with a extensive review ofdi erent concepts that are applicable to the problem, including adaptivitymechanisms (incluing QoS and load balancing), middleware and relevantdesign approaches (component-based, model-based and architectural design).A middleware is a software layer that can be used in distributed systems,with the purpose of abstracting away distribution, and possibly other aspects,for the application developers. The DySCAS project had as a major goaldevelopment of middleware for self-configurable systems in the automotivesector. Such development is complicated by the special requirements thatapply to these platforms.Work on the implementation of an adaptive middleware, DyLite, providingself-configurability to small-scale microcontrollers, is described andcovered in detail. DyLite is a partial implementation of the concepts developedin DySCAS.Another area given significant focus is formal modeling of QoS andresource management. Currently, applications in these types of systems arenot given a fully formal definition, at least not one also covering real-timeaspects. Using formal modeling would extend the possibilities for verificationof not only system functionality, but also of resource usage, timing and otherextra-functional requirements. This thesis includes a proposal of a formalismto be used for these purposes.Several challenges in providing methodology and tools that are usablein a production development still remain. Several key issues in this areaare described, e.g. version/configuration management, access control, andintegration between di erent tools, together with proposals for future workin the other areas covered by the thesis.
Utveckling av mjukvara för inbyggda realtidssystem innebär flera utmaningar.Hårda och mjuka tidskrav, och vanligtvis betydande resursbegränsningar,innebär viktiga inskränkningar på utvecklingen. Det traditionellasättet att hantera dessa utmaningar är att skapa en helt statisk design, d.v.s.en som är helt fix efter utvecklingsskedet.Dagens trender i området inbyggda system, inräknat trenden mot systemöppenhet,skapar nya utmaningar för systemens konstruktörer – exempelvisintegration av ny mjukvara under körskedet, uppgradering av mjukvaraeller anpassning av applikationsbeteende under körskedet för att nå bättreprestanda kombinerat med e ektivare resursutnyttjande. Ett sätt att nå dessamål är att bygga självkonfigurerande system, d.v.s. system som kan lösa sådanautmaningar utan mänsklig inblandning. Sådana mekanismer kan användas föratt öka systemens öppenhet.Denna avhandling täcker några av utmaningarna i denna utveckling. Enöversikt av den nuvarande situationen ges, med en omfattande genomgångav olika koncept som är relevanta för problemet, inklusive anpassningsmekanismer(inklusive QoS och lastbalansering), mellanprogramvara och relevantadesignansatser (komponentbaserad, modellbaserad och arkitekturell design).En mellanprogramvara är ett mjukvarulager som kan användas i distribueradesystem, med syfte att abstrahera bort fördelning av en applikation överett nätverk, och möjligtvis även andra aspekter, för applikationsutvecklarna.DySCAS-projektet hade utveckling av mellanprogramvara för självkonfigurerbarasystem i bilbranschen som ett huvudmål. Sådan utveckling försvåras avde särskilda krav som ställs på dessa plattformarArbete på implementeringen av en adaptiv mellanprogramvara, DyLite,som tillhandahåller självkonfigurerbarhet till småskaliga mikrokontroller,beskrivs och täcks i detalj. DyLite är en delvis implementering av konceptensom utvecklats i DySCAS.Ett annat område som får särskild fokus är formell modellering av QoSoch resurshantering. Idag beskrivs applikationer i dessa områden inte heltformellt, i varje fall inte i den mån att realtidsaspekter täcks in. Att användaformell modellering skulle utöka möjligheterna för verifiering av inte barasystemfunktionalitet, men även resursutnyttjande, tidsaspekter och andraicke-funktionella krav. Denna avhandling innehåller ett förslag på en formalismsom kan användas för dessa syften.Det återstår många utmaningar innan metodik och verktyg som är användbarai en produktionsmiljö kan erbjudas. Många nyckelproblem i områdetbeskrivs, t.ex. versions- och konfigurationshantering, åtkomststyrning ochintegration av olika verktyg, tillsammans med förslag på framtida arbete iövriga områden som täcks av avhandlingen.
DySCAS
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Santos, Athila Quaresma. "Um novo esquema para rejeição de cargas baseado em um sistema multiagentes." Universidade de São Paulo, 2016. http://www.teses.usp.br/teses/disponiveis/18/18154/tde-18082016-133244/.
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Esquemas de Rejeição de Cargas (RC) por subfrequência, utilizados para manter a frequência de operação de um Sistema Elétrico de Potência (SEP) próxima ao seu valor nominal, precisam ser criteriosamente projetados a fim de diminuir os riscos de colapso generalizado do sistema. Entretanto, pelos métodos convencionais, a quantidade de carga a ser rejeitada não leva em consideração a dinamicidade intrínseca do sistema, sendo baseada em conjecturas estáticas sobre porções do SEP. Como resultado, a redução da carga geralmente não é eficiente, gerando rejeição insuficiente ou excessiva. Neste cenário, este trabalho propõe um novo esquema para o controle da frequência em comparação aos processos de RC usualmente empregados. Com o propósito de superar as limitações e melhorar as principais funções desses processos é proposto um Sistema Multi Agentes (SMA) centralizado que irá coordenar as diversas etapas de monitoramento, processamento e tomada de decisão nos barramentos disponíveis para corte em situações de subfrequência. Busca-se dessa forma, desconectar o menor montante de cargas do sistema, por um curto espaço de tempo e com menor perturbação da frequência. Neste sentido, uma malha de controle fechada foi desenvolvida a partir da simulação de um sistema elétrico de potência completo via o Real Time Digital Simulator (RTDS). O SMA foi embarcado em um sistema integrado de hardware e software em tempo real para teste e validação da metodologia proposta. No contexto delineado, uma métrica de avaliação foi proposta para comparar o método proposto com outras duas filosofias convencionais de RC. Os resultados obtidos permitem evidenciar o bom desempenho do SMA frente às duas filosofias convencionais, principalmente no que se refere ao montante de carga a ser rejeitado, com boa aproximação do valor esperado.Automatic Under Frequency Load Shedding (AUFLS) schemes, used to maintain the frequency of an electric power system close to the nominal value, need to be carefully designed in order to reduce the risk of a widespread system collapse. However, the conventional methods do not take into account the inherent dynamics of an electric system and they are based on static assumptions. As a result, the shedding is generally not efficient, causing insufficient or excessive load discontinuity. In this scenario, this work proposes a new scheme for controlling the frequency compared to the AUFLS processes usually employed. In order to overcome the limitations of the methods usually employed and to improve the main functions of the AUFLS schemes, this work proposes a centralized MultiAgent System (MAS) that will coordinate the various stages of the monitoring and decision making process. The MAS seeks to disconnect a minimum amount of loads, in a short period of time and with less disturbance of the system frequency. A Hardware in Loop (HIL) configuration was developed from the simulation of a full electric system using the Real Time Digital Simulator (RTDS). The MAS was embedded in a real time system, consisting of hardware and software to test and validate the proposed methodology. In addition, a scoring metric evaluation is defined in order to compare other two conventional AUFLS philosophies. The results show good performance of the proposed MAS. The shedding was carried out in a single step and the amount of load shed was very close to the expected value.
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Alam, Mohammad Saad. "Real-time maximum power tracking and robust load matching of a stand-alone photovoltaic system a dissertation presented to the faculty of the Graduate School, Tennessee Technological University /." Click to access online, 2009. http://proquest.umi.com/pqdweb?index=0&did=1756844361&SrchMode=1&sid=2&Fmt=6&VInst=PROD&VType=PQD&RQT=309&VName=PQD&TS=1280170042&clientId=28564.
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Largura, Alvaro. "Desenvolvimento e avaliação de um sistema automatizado biosseguro para o tratamento, reciclagem e descarte de resíduo de microbiologia clínica." Universidade de São Paulo, 2007. http://www.teses.usp.br/teses/disponiveis/9/9136/tde-15042008-091817/.
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No Brasil, diariamente, são descartadas 2,3 toneladas de meios de cultura potencialmente contaminados com microorganismos. A resolução RDC No 306/2004 da Agência Nacional de Vigilância Sanitária preconiza que os resíduos devem ser tratados antes do descarte, visando à redução da carga microbiana. Foram desenvolvidos 2 métodos para avaliar a sensibilidade e a eficiência, assim como a concentração ideal, de um agente químico (biocida) contra microorganismos contaminantes. O método de difusão com Perfurador Circular de Ágar (PCA) e o método com Perfurador Linear de Ágar (PLA) foram testados com 13 cepas de microorganismos. O biocida avaliado foi a combinação de hipoclorito de sódio (NaClO) com ácido acético (CH3COOH). A partir destes resultados, foi desenvolvido um equipamento automatizado para processar a redução da carga microbiana (SADEMC) dos meios de cultura contaminados. A redução da carga microbiana foi avaliada pelo método quantitativo da reação da transcriptase reversa com detecção em tempo real. O método PCA mostrou ser reprodutível e eficiente para medir a inibição do crescimento bacteriano de um biocida. A concentração mínima de biocida capaz de reduzir o crescimento microbiano foi de 250 ppm para a solução aquosa de NaClO a 0,25% e de 200 ppm para a de CH3COOH a 0,2%. No SADEMC, foi possível processar 4,6kg de meios de culturas em 100 litros da concentração mínima eficaz do biocida por 15 minutos, e atingir uma redução da carga microbiana de, aproximadamente, 1,4E10 unidades formadoras de colônias. Podemos concluir que o SADEMC promove uma redução de carga microbiana compatível com os níveis exigidos pela RDC No. 306; fornece biossegurança na sua manipulação e que resulta em plástico reciclável.In Brazil, daily, 2.3 tons of potentially contaminated cultured medium with microorganisms are discarded. The RDC 306 resolution from the Brazilian National Health Department rules out that residue must be treated prior to discart in order to reduce microbial load. Two methods were developed to evaluate the sensitivity, efficiency and ideal concentration of a chemical agent (biocide) against microorganisms. The Ágar\'s Diffusion Method by Circular Perforator (PCA) and by Linear Perforator (PLA) were tested with 13 microorganism lines and the biocide composed by Sodium Hypochlorite (NaClO) and its combination with Acetic Acid (CH3COOH). The microbial load reduction was evaluated by the real time reverse transcription-polymerase chain reaction. From the in vitro data, an automatic equipment to process the potentially contaminated culture media (SADEMC) was developed. The PCA method was reproductive and efficient to measure the bacterial growth inhibition induced by the biocide. The minimum biocide concentration capable to reduce the microbial growth was a solution of 0.25% NaClO (250 ppm) and 0.2% CH3COOH (200 ppm). In the SADEMC, the direct exposition of 4.6 kg of culture media in 100 liters of biocide for a period of 15 minutes is capable to reduce the microbial load in approximately 1,4E10 of colony-forming unit. We may conclude that the SADEMC is able to promote a microbial load reduction more intense than the one demanded by the RDC 306 resolution. In addition to that, the SADEMC contemplates personnel safety and allows recycling the plastic residues
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Diniz, Ana Carolina Parise. "O uso de ferramentas de simulação humana para a avaliação de cargas físicas de trabalho: uma análise comparativa entre a aplicação em ambiente real na indústria aeronáutica e ambiente digital." Universidade Federal de São Carlos, 2014. https://repositorio.ufscar.br/handle/ufscar/3764.
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Previous issue date: 2014-05-20This research introduces the use of digital human modeling packages Tecnomatic members (Simens) - Jack software - and Dassout System - Delmia software. This choice is justified by the representation of these packages in terms of industrial applications as well as by the availability of such PSPLab / DEP / UFSCar. The overall study aims: to assess the variability in responses between the results of both software and application of RULA, NIOSH and Snook and Ciriello tools in real environment. And as a specific goal: to characterize the application of these tools in both softwares and compare the performance of different softwares. While search procedures were selected common three softwares analysis tools (RULA, NIOSH and Snook and Ciriello) and applied the same work situations. As base of applications and modeling in software performed the procedures prescribed for application of the tools were made by the researcher. Thus, the same situation was analyzed in three contexts: application of the tool by the researcher in a real environment (P), in Delmia (D) and in Jack (J). The activities were selected according to what each tool aims to analyze. It was observed that the results of the RULA and Snook and Ciriello real environment and digital applications have been coming, since the results of the NIOSH applications in both environments were discrepant . It is concluded that the RULA and Snook and Ciriello tools can be applied in human simulations in softwares Delmia and Jack to assist ergonomic analysis of actual and future activities. Further studies are needed to validate the application of the NIOSH software.
Esta pesquisa apresenta a utilização das modelagens humanas digitais integrantes dos pacotes Tecnomatic/Simens (software Jack) e Dassout System (software Delmia). Tal escolha é justificada pela representatividade destes pacotes em termos de aplicações industriais bem como pela disponibilidade dos mesmos no PSPLab/DEP/UFSCar. O estudo teve como objetivo geral: avaliar a variabilidade nas respostas entre os resultados dos dois softwares e da aplicação em ambiente real das ferramentas RULA, NIOSH e Snook e Ciriello. E como objetivo específico: caracterizar a aplicação destas ferramentas e comparar o desempenho dos diferentes softwares. Enquanto procedimentos de pesquisa foram selecionadas três ferramentas de análise comuns aos softwares (RULA, NIOSH e Snook e Ciriello) e aplicadas nas mesmas situações de trabalho. Como balizador das aplicações e modelagens realizadas nos softwares, os procedimentos prescritos para aplicação das ferramentas foram realizados pelo pesquisador. Assim, uma mesma situação foi analisada em três contextos: aplicação das ferramentas pelo pesquisador em ambiente real (P), no Delmia (D) e no Jack (J). As atividades foram selecionadas de acordo com o que cada ferramenta visa analisar. Foi possível observar que os resultados das aplicações do RULA e do Snook e Ciriello em ambiente real e digital foram próximos, já os resultados das aplicações da NIOSH em ambos ambientes foram discrepantes. Conclui-se então que as ferramentas RULA e Snook e Ciriello podem ser aplicadas em simulações humanas nos softwares Delmia e Jack para auxiliar na análise ergonômica. E mais estudos são necessários para validar a aplicação da NIOSH nos softwares.
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Cezar, Amanda Cristina [UNIFESP]. "Comparação de métodos convencionais e reação em cadeia da polimerase em tempo real na detecção de infecção pelo citomegalovírus in vitro." Universidade Federal de São Paulo (UNIFESP), 2009. http://repositorio.unifesp.br/handle/11600/9211.
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Previous issue date: 2009-09-30Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Introdução: Isolados clínicos do Citomegalovirus (CMV) são facilmente propagados in vitro resultando em comprometimento da monocamada celular onde o vírus foi inoculado, evidenciando assim a presença ou ausência de infecção. A cultura celular é um método clássico para detecção do CMV e foi bastante utilizada no passado. O ensaio de antigenemia, que detecta o antígeno viral pp65 do CMV, é o método mais utilizado atualmente na prática clínica, por ser mais rápido e específico para detecção da infecção ativa. Recentemente a técnica de PCR em tempo real tem sido empregada no monitoramento da infecção por meio da quantificação da carga viral por ser um método de alta sensibilidade e especificidade ao DNA viral. Sendo assim, o objetivo do estudo foi empregar testes usados no diagnóstico e monitoramento da infecção clínica à cepa padrão do CMV como protocolo para implantação em experimentos in vitro. Métodos: Monocamada de células fibroblásticas humanas confluentes e em quiescência foram inoculadas com amostras de células infectadas pela cepa adaptada em laboratório do CMV AD169. O efeito do vírus sobre a cultura foi monitorado 1 hora, 24 horas, 48 horas e 72 horas após a infecção (h.p.i) através da observação do efeito citopático. As mesmas amostras foram analisadas por antigenemia estimando-se a média de células positivas em 2x105 células e por PCR em tempo real estimando-se a média de cópias de DNA viral/Log10 presente nas amostras. Resultados: Efeito citopático foi observado pela primeira vez 24 h.p.i, evidenciando que o início das mudanças morfológicas ocorreu precocemente. Esse efeito tornou-se mais intenso após 72h. O ensaio de antigenemia evidenciou presença de infecção ativa pelo padrão de marcação do antígeno viral pp65 encontrado no núcleo das células infectadas, enquanto que a PCR em tempo real evidenciou o número de cópias de DNA viral nos diferentes tempos de infecção. Antigenemia apresentou uma média 57 ±56 células positivas 1h.p.i. O pico da infecção foi alcançado 24h.p.i com um aumento significativo da média para 2.381 ±168 (P<0.05 versus 1h.p.i), mantendo-se elevado 48h.p.i, mostrando uma média de 2.012 ±352. Entretanto, os níveis de antigenemia diminuem significativamente 72h.p.i para 262 ±5 (P<0.05 versus 48h.p.i). Assim como na antigenemia, observou-se aumento significativo da carga viral de 1 h.p.i para 24 h.p.i, sendo uma média de DNA viral detectado 11.30 ±0.30 e 11.96 ±0.09, respectivamente (P<0.05 versus 1h.p.i). Os níveis de DNA viral se mantêm elevados 48h.p.i, sendo detectada uma média de 12.33 ±0,26. Após esse período, carga viral cai significativamente para 11.57 ±0.06 (P<0.05 versus 48h.p.i). Não foi encontrada correlação entre os métodos quantitativos de antigenemia e PCR em tempo real. Conclusão: Os três métodos utilizados, isolamento viral, antigenemia e PCR em tempo real evidenciaram o sucesso da infecção “in vitro” pelo CMV por meio de mudanças cito-morfológicas, detecção de antígeno viral específico e carga viral por detecção do DNA viral, respectivamente. A técnica de PCR se mostrou a mais sensível na detecção viral em relação às demais técnicas. Embora sejam métodos sensíveis e específicos, consideramos a necessidade da titulação viral em quaisquer ensaios experimentais in vitro.
Introduction: Clinical isolates of Cytomegalovirus (CMV) are easily spread in vitro resulting in impairment of the monolayer cell where the virus was inoculated, thus evidencing the presence or absence of infection. The cell culture is a classic method for detection of CMV and it was widely used in the past. Antigenemia assay, which detects CMV pp65 antigen, is the method most used currently in clinical practice, because it is faster and specific for detection of the active infection. Recently, the real-time PCR has been used in monitoring of the infection through the quantification of viral load for being a high sensitivity and specificity method to viral DNA. Therefore, the aim of the study was employing tests used in diagnosis and monitoring of infection to the standard CMV strain as a protocol for implantation in experiments in vitro. Methods: Quiescent human fibroblasts in confluent monolayer were inoculated with samples of infected cells by the adapted CMV AD169 strain. The effect of the virus on culture was monitored at 1 hour, 24 hours, 48 hours and 72 hours post infection (h.p.i) by observation of cytopathic effect. The same samples were analyzed by antigenemia being estimate the mean of positive cells in 2x105 cells and by real-time PCR being estimate the mean of copies of viral DNA/Log10 present in samples. Results: Cytopathic effect was first noticed 24 h.p.i, showing that the initiation of morphological changes occurred early. This effect became more intense after 72 h.p.i. Antigenemia assay showed the presence of active infection through pattern of labeling of the pp65 viral antigen found on nucleus of infected cells, while the real-time PCR showed the number of copies of viral DNA in different times of infection. Antigenemia showed an mean of 57 ±56 positive cells 1h.p.i. The peak of the infection was reached 24h.p.i with a significant increase in the mean 2.381 ±168 (P<0.05 versus 1h.p.i) and remained high 48h.p.i, showing an mean of 2.012 ±352 positive cells. However, the mean of antigenemia decrease 72h.p.i to 262 ±5 (P<0.05 versus 48h.p.i). As well as in antigenemia, a significant increase of th viral load was observed of 1h.p.i to 24h.p.i, being the mean of viral DNA detected 11.30 ±0.30 and 11.96 ±0.09, respectively (P<0.05). The levels of viral DNA stayed high 48h.p.i, being detected a mean of 12.33 ±0.26. After this period, viral load decreased significantly to 11.57 ±0.06 (P<0.05 versus 48h.p.i). No correlation was found between the quantitative methods of antigenemia and real-time PCR. Conclusion: The three methods, virus isolation, antigenemia and real-time PCR, showed the success of the CMV infection “in vitro” by cyto-morphological changes, detection of viral antigen specific and viral load by virus DNA detection, respectively. PCR method was more sensitive in detecting virus in relation the other methods. Although sensitive and specific, we consider the need for viral titration in any experimental studies in vitro.
TEDE
BV UNIFESP: Teses e dissertações
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Westerberg, Jacob. "Active Phase Balancing and Battery Systems for Peak Power Reduction in Residential Real Estate : An Economic Feasibility Study." Thesis, KTH, Industriell Management, 2020. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-272974.
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Research has shown that three-phase balancing alone can improve the operation of secondary distribution networks and that the addition of energy storage to the phase balancing power electronics further helps to alleviate the negative effects of phase unbalances. However, less attention has been paid to the economic potential of said technologies and particularly for loadside implementation. It appears that the deployment of phase balancers, with or without energy storage, is indeed hampered by uncertainty related to its economic feasibility, despite both technologies being commercially available. This thesis therefore aims to assess and compare the economic feasibility of the two configurations for peak shaving purposes in the context of residential property loads in Sweden. The assessment was performed using a specially developed deterministic techno-economic model taking into consideration historical load data from three Swedish real estate, cost estimations for a range of alternatives used when sizing the systems, applicable tariffs and fees for electricity and its distribution as well as technical parameters such as the capacities and efficiencies of the involved components. A novel approach was taken by linearly extrapolating the three load profiles into three sets of 91 synthesized load profiles to enable a larger dataset for analysis. The net present values generated for each set were then graphed and analyzed per original real estate. The results showed that both configurations can be economically feasible, but only under certain conditions. A phase balancer alone was found to be feasible for real estate whose peak currents are distinctly unbalanced and exceed 50 A, with the best expected rate of return for profiles exceeding 63 A since they enable a tariff switch. The combined system was found to be even more contingent on the tariff switch and therefore only feasible for peaks above 63 A. A substantial difference in the initial investment further makes the single phase balancer the preferred choice, unless the discount rate is as low as 2 % or less. On this basis, potential investors need to assess the state of unbalance of their loads and perform their own calculation based their load profile, cost of capital and applicable tariffs.Tidigare forskning har visat att fasbalansering enskilt kan förbättra driften hos lokala distributionsnät och att ett batterisystem i tillägg till fasbalanserarens kraftelektronik ytterligare kan minska de negativa effekterna av fasobalanser. Däremot har mindre uppmärksamhet riktats mot den ekonomiska genomförbarheten hos dessa teknologier och i synnerhet för implementation på lastens sida av elmätaren. Det tycks vara så att spridningen av fasbalanserare, med eller utan energilagring, hindras av osäkerheten kring dess ekonomiska potential trots att båda teknologierna är kommersiellt tillgängliga. Detta arbete ämnar därför att värdera och jämföra den ekonomiska nyttan hos de två konfigurationerna vid toppreducering av fastighetselen i svenska bostadsfastigheter. Värderingen utfördes med hjälp av en särskilt utvecklad deterministisk tekno-ekonomisk modell som beaktade historiska lastdata från tre svenska fastigheter, kostnadsuppskattningar för en uppsättning av konfigurationer som användes vid dimensionering av systemen, applicerbara tariffer och avgifter för elektricitet och dess distribution samt tekniska parametrar såsom kapaciteter och verkningsgrader för de olika komponenterna. Ett annorlunda tillvägagångssätt tillämpades vidare för att utöka datamängden genom linjär extrapolation av lastprofilerna, vilket resulterade i tre uppsättningar av 91 syntetiserade lastprofiler. Nettonuvärdet beräknades följaktligen för varje profil och investeringsalternativ för att sedan plottas och analyseras per ursprunglig fastighet. Resultaten visade att båda konfigurationerna kan uppvisa lönsamhet, men endast under särskilda förutsättningar. Den enskilda fasbalanseraren bedömdes som lönsam för fastigheter vars strömtoppar är påtagligt obalanserade och som överstiger 50 A, med största möjliga lönsamhet för profiler som överstiger 63 A då dessa möjliggör ett tariffbyte. Det kombinerade systemets lönsamhet bedömdes vara ännu mer beroende av tariffbytet och därför endast lönsamt för strömtoppar över 63 A. En betydligt större grundinvestering för det kombinerade systemet gör vidare att den enskilda fasbalanseraren i regel är att föredra, såvida inte kalkylräntan är så låg som 2 % eller mindre. Baserat på detta uppmanas potentiella investerare att undersöka balanstillståndet hos deras laster och att utföra en egen kalkyl baserat på deras specifika last, kapitalkostnad och nätföretag.
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Cheng, Danling. "Integrated System Model Reliability Evaluation and Prediction for Electrical Power Systems: Graph Trace Analysis Based Solutions." Diss., Virginia Tech, 2009. http://hdl.handle.net/10919/28944.
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A new approach to the evaluation of the reliability of electrical systems is presented. In this approach a Graph Trace Analysis based approach is applied to integrated system models and reliability analysis. The analysis zones are extended from the traditional power system functional zones. The systems are modeled using containers with iterators, where the iterators manage graph edges and are used to process through the topology of the graph. The analysis provides a means of computationally handling dependent outages and cascading failures. The effects of adverse weather, time-varying loads, equipment age, installation environment, operation conditions are considered. Sequential Monte Carlo simulation is used to evaluate the reliability changes for different system configurations, including distributed generation and transmission lines. Historical weather records and loading are used to update the component failure rates on-the-fly. Simulation results are compared against historical reliability field measurements.
Given a large and complex plant to operate, a real-time understanding of the networks and their situational reliability is important to operational decision support. This dissertation also introduces using an Integrated System Model in helping operators to minimize real-time problems. A real-time simulation architecture is described, which predicts where problems may occur, how serious they may be, and what is the possible root cause.Ph. D.
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Modena, José Luiz Proença. "Infecções respiratórias por bocavirus humano: aspectos clínicos e moleculares." Universidade de São Paulo, 2009. http://www.teses.usp.br/teses/disponiveis/17/17136/tde-29052009-143332/.
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O bocavirus humano (HBoV) é um parvovirus recentemente identificado em associação com a presença de sintomas de infecção do trato respiratório. Esse vírus possui um genoma de aproximadamente 5217 nucleotídeos que contém 3 open reading frames que codificam 4 proteínas (NS1, NP-1, VP-1 e VP-2). HBoV tem sido detectado em amostras respiratórias de diversas partes do mundo, incluindo Austrália, América do Norte, Europa, Ásia e África, o que sugere uma distribuição global desse vírus. Entretanto, nenhum estudo longitudinal de HBoV em amostras respiratórias foi realizado na América Latina. Dessa forma, nós realizamos um estudo prospectivo de HBoV em lavados nasofaríngeos (LFNs) coletados de pacientes com sintomas de infecção do trato respiratório (IRA) atendidos em um hospital universitário de Ribeirão Preto, SP e em um hospital universitário de Salvador, BA no período entre 2005 a 2007. 1288 LFNs de 1217 pacientes foram encaminhados ao laboratório de virologia e foram testados por PCR para HBoV. Desses pacientes, 962 eram menores de 5 anos e 177 eram maiores de 5 anos. Além disso, também foram analisados 50 LFNs de crianças menores de 5 anos que não tinham sintomas respiratórios. Todas as amostras positivas para HBoV foram testadas para todos os outros vírus respiratórios, incluindo o vírus sincicial respiratório (HRSV), rinovirus humano (HRV), influenza humano (HFLU), metapneumovirus humano (HMPV), parainfluenza humano (HPIV), coronavirus humano (HCoV) e adenovirus humano (HAdV). A carga viral de HBoV foi determinada por PCR em tempo real em todas as amostras positivas e o genoma completo de 19 amostras de HBoV foi seqüenciado. Com intuito, de fazer um levantamento sorológico e determinar sítios replicativos de HBoV, nós ainda clonamos e expressamos em S. cerevisae (Y258) o gene de VP2, que codifica uma das proteínas do capsídeo viral. A prevalência desse vírus foi de 4,8% em crianças menores de cinco anos e de 1% em pacientes maiores de cinco anos. HBoV não foi detectado em crianças sem sintomas. Dos 259 pacientes analisados em 2005, 25 (10%) foram positivos para HBoV. Esse vírus circulou mais frequentemente em abril, mês de maior incidência do HRSV. Em 2006, HBoV foi detectado em apenas 10 LFNs de 334 (3%) amostras testadas, sem qualquer pico de freqüência. Em 2007 HBoV foi detectado em 13 de 552 (2%) amostras, com uma freqüência de detecção um pouco maior em junho e julho. Os sintomas mais comumente observados foram rinorréia, tosse, febre e chiado, que foram observados geralmente em mais de 50% dos casos positivos para HBoV. Não houve uma diferença significativa na prevalência desses sintomas entre as crianças positivas e negativas para HBoV. Entretanto, foi observada uma maior freqüência de diarréia entre as crianças com esse vírus. Nesse estudo também foi documentado uma alta freqüência de co-infecções virais entre os pacientes com HBoV. Os vírus mais frequentemente associados com o bocavirus humano foram: HRSV, HRV e HAdV. Além disso, foi detectado uma maior carga viral media e uma maior freqüência de diarréia nos 15 pacientes com infecção exclusiva por HBoV do que nos pacientes com co-infecção. Esses resultados mostraram que HBoV pode alcançar títulos enormes (tão grandes como1014/mL) em LFNs de pacientes com sintomas respiratórios e que isso é associado a de diarréia. O seqüenciamento do genoma inteiro de HBoV realizado nesse estudo indica que a divergência genômica entre as amostras desse vírus é muito pequena. Como conclusão, nós demonstramos que HBoV circula e é detectado em associação com sintomas de infecção respiratória e diarréia no Brasil. Novos estudos, com um longo acompanhamento em diferentes populações serão necessários para determinar a sazonalidade e o real impacto clínico de HBoV em nosso país.Human bocavirus (HBoV) is a parvovirus recently identified in association with respiratory tract infections. HBoV 5217 nt genome contains 3 open reading frames encoding four proteins (NS1, NP-1, VP-1 and VP-2). HBoV has been reported in respiratory samples from children in several parts of the world (including Australia, North America, Europe, Asia, and Africa), suggesting that the virus circulates worldwide. However, no longitudinal studies of HBoV in respiratory samples have been reported in Latin America. We report a prospective study of HBoV in nasopharyngeal aspirates (NPAs) collected from patients seen for acute respiratory tract infections (ARI) at the University of Sao Paulo Hospital in Ribeirao Preto, southeast Brazil and at the University Hospital in Salvador, Brazil. 1288 NPAs from 1217 patients was submitted to the virology lab for respiratory virus detection from 2005 to 2007 and were screened for HBoV by polymerase chain reaction (PCR), whom 962 were under 5 years of age and 177 were older than 5 years. In addition, NPAs from 50 children under 12 years without IRA was also tested to HBoV for PCR. All samples positive of HBoV was tested for others respiratory virus, including the human respiratory syncitial virus (HRSV), human rhinovirus (HRV), human influenza (HFLU), human metapneumovirus (HMPV), human parainfluenza virus (HPIV), human coronavirus (HCoV) and human adenovirus (HAdV). These samples had their HBoV viral load determined by real time PCR and the viral entire genome of nineteen HBoV sample was sequenced. We also cloned and expressed in S. cerevisae (Y258) the gene of VP2, one protein of viral capside. The prevalence of this virus was of 4,8% in children under 5 years and 1% in adults, both with IRA. HBoV was not found on the patients without symptoms. In 2005, of the 259 patients tested, 25 (10%) were positive for HBoV. Interestingly, the virus circulated more frequently in April, the month of peak activity of respiratory HRSV. In 2006 HBoV was detected in only 10 NPAs out of 334 samples (3%) tested, without any notable peak of frequency. In 2007 HBoV was detected in 13 out of 552 (2%) tested samples with little higher frequency of detection in June an July. Rhinorrhea, cough, and wheezing were observed in more than 50% of the HBoV-positive children, and no obvious respiratory clinical differences were noted between HBoV-positive and negative children. However, was noted a higher frequency of diarrhea on HBoV-positive patients. In this study was also observed a larger frequency (71%) of viral coinfections between the HBoV-positive patients. The respiratory viruses more frequently associated with human bocavirus were: HRSV, HRV and HAdV. Interestingly, on the 15 HBoV-alone patients was observed a higher viral load and a higher prevalence of diarrhea than HBoV-coinfection patients. These results showed that this virus can reach enormous titles (like 1014) in NPAs from patients with respiratory infection symptoms and this is associated with diahhrea. The entire genome sequencing of HBoV of our study indicates that the genetic divergence between the HBoV lineages is small. In conclusion, we demonstrated that HBoV circulates and is detected in association with respiratory symptoms and diarrhea in Brazil. Long term surveillance will be needed to determine whether or not an HBoV season occurs and what is the real clinical impact of this virus in our country.
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Van, der Bijl Johannes. "Sustainable DSM on deep mine refrigeration systems : a novel approach / J. van der Bijl." Thesis, North-West University, 2007. http://hdl.handle.net/10394/1940.
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Goyal, Sachin. "Power network in the loop : subsystem testing using a switching amplifier." Queensland University of Technology, 2009. http://eprints.qut.edu.au/26521/.
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“Hardware in the Loop” (HIL) testing is widely used in the automotive industry. The sophisticated electronic control units used for vehicle control are usually tested and evaluated using HIL-simulations. The HIL increases the degree of realistic testing of any system. Moreover, it helps in designing the structure and control of the system under test so that it works effectively in the situations that will be encountered in the system. Due to the size and the complexity of interaction within a power network, most research is based on pure simulation. To validate the performance of physical generator or protection system, most testing is constrained to very simple power network. This research, however, examines a method to test power system hardware within a complex virtual environment using the concept of the HIL. The HIL testing for electronic control units and power systems protection device can be easily performed at signal level. But performance of power systems equipments, such as distributed generation systems can not be evaluated at signal level using HIL testing. The HIL testing for power systems equipments is termed here as ‘Power Network in the Loop’ (PNIL). PNIL testing can only be performed at power level and requires a power amplifier that can amplify the simulation signal to the power level. A power network is divided in two parts. One part represents the Power Network Under Test (PNUT) and the other part represents the rest of the complex network. The complex network is simulated in real time simulator (RTS) while the PNUT is connected to the Voltage Source Converter (VSC) based power amplifier. Two way interaction between the simulator and amplifier is performed using analog to digital (A/D) and digital to analog (D/A) converters. The power amplifier amplifies the current or voltage signal of simulator to the power level and establishes the power level interaction between RTS and PNUT. In the first part of this thesis, design and control of a VSC based power amplifier that can amplify a broadband voltage signal is presented. A new Hybrid Discontinuous Control method is proposed for the amplifier. This amplifier can be used for several power systems applications. In the first part of the thesis, use of this amplifier in DSTATCOM and UPS applications are presented. In the later part of this thesis the solution of network in the loop testing with the help of this amplifier is reported. The experimental setup for PNIL testing is built in the laboratory of Queensland University of Technology and the feasibility of PNIL testing has been evaluated using the experimental studies. In the last section of this thesis a universal load with power regenerative capability is designed. This universal load is used to test the DG system using PNIL concepts. This thesis is composed of published/submitted papers that form the chapters in this dissertation. Each paper has been published or submitted during the period of candidature. Chapter 1 integrates all the papers to provide a coherent view of wide bandwidth switching amplifier and its used in different power systems applications specially for the solution of power systems testing using PNIL.
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Saeidpour, Parizy Ehsan. "Electrical Energy Retail Price Optimization for an Interconnected/Islanded Power Grid." University of Akron / OhioLINK, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=akron1512463830323059.
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Targa, Lilia Spaleta. "Estudo da carga parasitária e dos genótipos de Toxoplasma gondii na toxoplasmose congênita." Universidade de São Paulo, 2013. http://www.teses.usp.br/teses/disponiveis/99/99131/tde-06092013-152217/.
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O genótipo e a carga parasitária constituem dois dos principais fatores associados à patogênese na toxoplasmose congênita. Na Europa e nos EUA, o genótipo II é o mais prevalente em infecções congênitas, enquanto que na América do Sul existem evidências apontando uma maior frequência de genótipos atípicos ou recombinantes, associados a casos mais graves. A carga parasitária também parece atuar como fator de risco independente associado ao prognóstico fetal. Os objetivos do estudo foram padronizar uma amplificação quantitativa (qPCR) com iniciadores do gene B1 para avaliar a carga parasitária; determinar o genótipo parasitário por multiplex-nested-PCR-RFLP dos marcadores 5\'-SAG2, 3\'-SAG2, SAG3 e GRA6, seguido de sequenciamento para confirmação da RFLP e análise de mutações; e verificar se existiria associação entre a carga parasitária e os genótipos parasitários nas mesmas gestações. Foram analisadas 76 amostras de líquido amniótico de gestações com toxoplasmose e 31 amostras controle. A qPCR apresentou LOD de 10 parasitos/mL, detectou as 76 amostras de estudo e nenhum controle. As cargas parasitárias variaram de 222 a 808.328 parasitos/mL. Houve duas amostras com valores acima de 104 parasitos/mL, apesar de todas as gestantes serem tratadas. Na genotipagem, SAG3 amplificou 55 amostras (54 tipo III e uma tipo II); 5\' e 3\'-SAG2 amplificaram 54 amostras (todas tipo I), e GRA6, amplificou 20 amostras (todas tipo III). A única amostra com genótipo parasitário SAG3-tipo II foi a que apresentou mais mutações (n=4), carga parasitária de 958 parasitos/mL, porém o recém-nascido foi assintomático. Houve diferença do número de amostras amplificadas por SAG3, e 5\' e 3\'-SAG2 em relação a GRA6 (McNemar, p<0,001). Os sequenciamentos confirmaram 100% dos resultados de RFLP, e foram encontradas 24 amostras com e 52 sem mutações, não existindo diferenças entre as cargas parasitárias dos dois grupos (Mann-Whitney, p= 0,085). Mais de uma mutação foi observada em cinco amostras. Foram detectadas 37 mutações no estudo: 26 heterozigotas/sinônimas e 11 homozigotas/sinônimas, não havendo regiões hot spot. Quanto à correlação clínico-laboratorial, dos 76 recém-nascidos, todos apresentaram IgM positiva ao nascimento, e 75 eram assintomáticos. O único recém-nascido sintomático apresentava tríade de Sabin e uma das duas cargas parasitárias mais elevadas do estudo (309.574 parasitos/mL), porém o genótipo não foi discriminante e não havia mutações. A outra amostra com carga parasitária acima de 104 parasitos/mL pertencia a recém-nascido assintomático, com genótipo não discriminante, e sem mutações. O estudo concluiu que a técnica de Real Time PCR (qPCR) foi padronizada com sucesso, usando os iniciadores B22 e B23 do gene B1 do parasito, podendo ser empregada na rotina diagnóstica. Além disso, foi possível realizar a genotipagem das amostras incluídas no estudo, com melhor desempenho de SAG3 e 5\' e 3\'-SAG2. O sequenciamento confirmou a confiabilidade da técnica de RFLP, e encontrou frequência elevada de mutações, todas sinônimas, sem regiões hot spot, e aparentemente sem associação com a carga parasitária. Houve elevada variabilidade das cargas parasitárias, porém grande homogeneidade dos genótipos parasitários, não tendo sido observada associação entre a carga parasitária e os genótipos de T. gondii no estudo.The genotype and the parasite load are two of the main factors associated with pathogenesis in congenital toxoplasmosis. In Europe and the USA, genotype II is the most prevalent in congenital infections, while in South America there is evidence pointing to a higher frequency of atypical or recombinant genotypes associated with more severe cases. The parasite load also appears to act as an independent risk factor associated with fetal prognosis. The study objectives were to standardize a quantitative amplification (qPCR) with B1 gene primers to assess the parasite load; determine the genotype by multiplex-nested-PCR-RFLP of 5\' and 3\'-SAG2, SAG3 and GRA6 markers, followed by sequencing to confirm RFLP and analyze mutations, verifying whether there is association between parasite load and parasite genotypes in the same pregnancies. We analyzed 76 amniotic fluid samples from pregnancies with toxoplasmosis and 31 controls. The qPCR presented LOD of 10 parasites/mL, detected the 76 study samples and no control. Parasite loads ranged from 222 to 808,328 parasites/mL. There were two samples with values above 104 parasites/mL, despite all pregnant women be treated. In genotyping, SAG3 amplified 55 samples (54 type III and 1 type II); 5 \'and 3\'-SAG2 amplified 54 samples (all type I); and GRA6, amplified 20 samples (all type III). The only sample with genotype SAG3-type II showed the highest number of mutations (n=4), parasite load of 958 parasites/mL, but the newborn was asymptomatic. There were differences in the number of samples amplified by SAG3, and 5 \'and 3\'-SAG2 over GRA6 (McNemar test, p <0.001). Sequencing confirmed 100% of the RFLP results; and found 24 samples with and 52 without mutations, with no difference between the parasite load of these two groups (Mann-Whitney, p= 0.085). More than one mutation was observed in five samples. A total of 37 mutations were detected in this study: 26 heterozygotes/synonymous and 11 homozygous/synonyms, with no hot spot regions. Regarding the clinical-laboratory correlation, among the 76 newborns, all showed positive IgM at birth, and 75 were asymptomatic. The only symptomatic newborn presented the Sabin\'s triad and one of the two higher parasite loads in the study (309,574 parasites/mL). However, the genotype was not discriminant and no mutations were detected. The other sample with parasite load above 104 parasites/mL belonged to an asymptomatic newborn with a non-discriminating genotype, and no mutations. The study concluded that the Real Time PCR (qPCR) was successfully developed with primers B22 and B23 of the parasite B1 gene, and can be used in routine practice. Moreover, it was possible to perform the samples genotyping, with better performance of SAG3 and 5 \'and 3\'-SAG2. Sequencing results confirmed the RFLP reliability, and found a high frequency of mutations, all synonymous, with no hot spot regions, and apparently not associated with the parasite load. There was a high variability in parasite load, however great homogeneity of parasite genotypes, with no association between the parasite load and T. gondii genotypes in the study.
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Arexis, Mahé. "La capture attentionnelle : «transposabilité » du phénomène du laboratoire au monde réel." Thesis, Bourgogne Franche-Comté, 2018. http://www.theses.fr/2018UBFCC010/document.
Full textAbstract:
Depuis une vingtaine d’années, la littérature scientifique traitant de la capture attentionnelle a mis en évidence, à l’aide de paradigmes expérimentaux testés en laboratoire, un certain nombre de processus attentionnels fondamentaux. Bien que les résultats obtenus « en laboratoire » à partir de stimuli visuellement « simples » méritent encore d’être investigués, depuis quelques années se pose la question de la « transposabilité » de ces observations à des objets et des situations issus du monde réel. Les phénomènes observés en laboratoire à partir d’un matériel visuellement simple sont-ils transposables à des situations, complexes, de la vie quotidienne ? Afin de répondre à cette question, nous avons créé et testé différentes conditions proches de celles du monde réel,notamment en expérimentant en situation de double-tâche, en utilisant un matériel visuellement complexe extrait du monde réel (c.-à-d. des photographies de conduite automobile), en faisant varier la fréquence d’apparition de l’élément distracteur ou bien encore en testant une nouvelle caractéristique du distracteur, la dimension sémantique. Nos résultats révèlent les conditions d’apparition du phénomène de capture attentionnelle dans des situations s’approchant de celles du monde réel. Nous avons tout particulièrement détaillé dans cet ouvrage le cas de l’effet de capture attentionnelle contingente, phénomène majeur et robuste de la capture attentionnelle, y compris dans des situations visuellement complexesDuring the last two decades, studies about attentional capture revealed some major basic attentional processes by using several experimental paradigms. While further investigations need to be conducted by using simple visual stimuli, a raising question concerns the possibility to generalize laboratory findings to much more complex real-world situations. Indeed, basic attentional capture studies usually use simple stimuli while real-world displays are generally rich in visual information. To answer this issue, we conducted several experiments under close to real-world conditions, such as testingdual task situations, using complex visual stimuli from real-world situations (e.g. driving-scenes photographs), modulating the distractor frequency or testing attentional capture at a semantic and conceptual dimension. Our results revealed the conditions in which the attentional capture phenomenon occurs in close to real-world situations. We particularly discussed in our work the contingent attentional capture phenomenon which appears to be a strong and robust effect, in both laboratory and close to real-world situations
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Allen, Vonetta. "Relationship Between Loan Product, Loan Amount, and Foreclosure After the Subprime Lending Crisis." ScholarWorks, 2017. https://scholarworks.waldenu.edu/dissertations/4414.
Full textAbstract:
Following the collapse of property values and an increasing rate of default on high-risk mortgages, the United States experienced a subprime lending crisis that led to massive financial losses for holders of mortgage-backed securities. The purpose of this correlational study was to examine if loan product and loan amount predict the likelihood of loan foreclosure. The theoretical framework grounding the study was Minsky's financial instability hypothesis, which describes the basis of capitalism as economic expansionism followed by financial crises. The population consisted of 473 loan cases from archival data of the Atlanta Sixth Federal Reserve District in Georgia. The method used to collect the data was a probabilistic simple random sample taken from the archival data. The use of binary logistic regression resulted in a finding that the variables of loan product and loan amount significantly predicted the likelihood of loan foreclosure, Ï?2(4) = 10.65, p = .031, Nagelkerke R2 = .09. The Nagelkerke R2 value indicated that the model explained 9% of the variability in foreclosure. The findings specifically showed that Federal Housing Authority and Veterans Administration loan products were significantly more likely than conventional loans to cause losses for mortgage lenders. The implications for positive social change include increased stakeholder knowledge of various factors that can contribute to foreclosure and sustainment of community value with fewer homeowners losing their home in foreclosure.
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Tangvald, Lars. "Implementing LOD for physically-based real-time fire rendering." Thesis, Norwegian University of Science and Technology, Department of Computer and Information Science, 2007. http://urn.kb.se/resolve?urn=urn:nbn:no:ntnu:diva-8814.
Full textAbstract:
In this paper, I present a framework for implementing level of detail (LOD) for a 3d physically based fire rendering running on the GPU. While realistic fire rendering that runs in real time exists, it is generally not used in real-time applications such as game, due to the high cost of running such a rendering. Most research into the rendering of fire is only concerned with the fire itself, and not how it can best be included in larger scenes with a multitude of other complex objects. I present methods for increasing the efficiency of a physically based fire rendering without harming its visual quality, by dynamically adjusting the detail level of the fire according to its importance for the current view. I adapt and use methods created both for LOD and for other areas to alter the detail level of the visualization and simulation of a fire rendering. The desired detail level is calculated by evaluating certain conditions such as visibility and distance from the viewpoint, and then used to adjust the detail level of the visualization and simulation of the fire. The implementation of the framework could not be completed in time, but a number of tests were run to determine the effect of the different methods used. These results indicate that by making adjustments to the simulation and visualization of the fire, large boosts in performance are gained without significantly harming the visual quality of the fire rendering.