Academic literature on the topic 'Real-time biosensors'

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Dissertations / Theses on the topic "Real-time biosensors"

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Ng, Shu Rui. "Electrochemical biosensors for real-time detection of angiogenesis." Thesis, Imperial College London, 2013. http://hdl.handle.net/10044/1/25625.

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Electrochemical biosensors have been made to detect the metabolic markers, pH, O2 and glucose, and nitric oxide (NO), the signalling molecule involved in angiogenesis. A novel three-dimensional (3-D) graphene/ionic liquid (IL) nanocomposite demonstrates highly sensitive detection of NO in phosphate buffered saline (PBS). An arginine-glycine-aspartic acid (RGD) peptide-functionalised biomimetic graphene film has been used as both a cell culture and sensing matrix to detect NO released by human umbilical vein endothelial cells (HUVECs) in real-time under acetylcholine (Ach) stimulation. The amou
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Phairatana, Tonghathai. "Bioengineering of novel carbon-based biosensors for real-time biomedical use." Thesis, Imperial College London, 2015. http://hdl.handle.net/10044/1/58345.

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The aim of this thesis was to develop novel carbon-based biosensors and sensors for real-time metabolite and drug detection, to provide the next generation of medical devices which can give clinicians relevant chemical information in real-time at the patient bedside. An autocalibration system was developed using LabSmith programmable components to give precise fluid delivery and excellent temporal control of multiple liquid streams. This enables a 5-point calibration to be carried out using two solutions in 12 minutes. Systems using chitosan, poly(ethylene glycol)diglycidyl ether hydrogel, ele
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Liu, Chang. "Localized Surface Plasmon Resonance Biosensors for Real-Time Biomolecular Binding Study." FIU Digital Commons, 2013. http://digitalcommons.fiu.edu/etd/837.

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Surface Plasmon Resonance (SPR) and localized surface plasmon resonance (LSPR) biosensors have brought a revolutionary change to in vitro study of biological and biochemical processes due to its ability to measure extremely small changes in surface refractive index (RI), binding equilibrium and kinetics. Strategies based on LSPR have been employed to enhance the sensitivity for a variety of applications, such as diagnosis of diseases, environmental analysis, food safety, and chemical threat detection. In LSPR spectroscopy, absorption and scattering of light are greatly enhanced at frequencies
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Stengel, Gudrun. "Real time monitoring of DNA hybridization and replication using optical and acoustic biosensors." [S.l. : s.n.], 2004. http://deposit.ddb.de/cgi-bin/dokserv?idn=971304572.

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Hong, Soonjin Barbee Kenneth A. "Quantitative analysis of cell-surface interactions and cell adhesion process in real-time /." Philadelphia, Pa. : Drexel University, 2008. http://hdl.handle.net/1860/2840.

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Canelle, Quentin. "Real Time Surface Plasmon Resonance Biosensors, a Powerful Technology to Assess Polyclonal Antibody Avidity." Doctoral thesis, Universite Libre de Bruxelles, 2015. http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/216754.

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The present research focused on the development of a new methodology to assess the strength of the interaction between vaccine antigens and elicited polyclonal antibodies through SPR biosensors. Quantifying the binding strength of polyclonal antibodies is of first importance to evaluate the quality of the vaccine as well as to increase the scientific knowledge of immune protection mechanisms. To now the development of such tool has been complicated by the non-specific binding caused by high protein abundance in the blood and serum samples but also by the way of interpreting the data resulting
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Zelada, Guillén Gustavo Adolfo. "Ultrasensitive detection of pathogens in real-time. Potentiometric biosensors based on single-walled carbon nanotubes and aptamers." Doctoral thesis, Universitat Rovira i Virgili, 2011. http://hdl.handle.net/10803/51768.

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Un gran nombre de plataformes de detecció biològica han incorporat materials nanoestructurats com una estratègia per a millorar diversos paràmetres operacionals i de qualitat tals com reduir els temps d'anàlisis i els límits de detecció. Les tècniques electroquímiques de detecció es prefereixen sobre altres tècniques ja que presenten una sèrie d'avantatges com a rapidesa, facilitat de maneig, cost reduït i la reduïda mida dels detectors comercials. Entre les tècniques electroquímiques, les metodologies més simples, comunes i més fàcils de transportar són aquelles basades en la potenciome
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Teerapanich, Pattamon. "Fluorescence-based nanofluidic biosensor platform for real-time measurement of protein binding kinetics." Thesis, Toulouse 3, 2015. http://www.theses.fr/2015TOU30239/document.

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L'analyse cinétique d'interactions de protéines offre une multitude d'informations sur les fonctions physiologiques de ces molécules au sein de l'activité cellulaire, et peut donc contribuer à l'amélioration des diagnostics médicaux ainsi qu'à la découverte de nouveaux traitements thérapeutiques. La résonance plasmonique de surface (SPR) est la technique de biodétection optique de référence pour les études cinétiques d'interaction de molécules biologiques. Si la SPR offre une détection en temps réel et sans marquage, elle nécessite en revanche des équipements coûteux et sophistiqués ainsi que
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Brawner, Keith. "Modeling Learner Mood in Realtime through Biosensors for Intelligent Tutoring Improvements." Doctoral diss., University of Central Florida, 2013. http://digital.library.ucf.edu/cdm/ref/collection/ETD/id/5774.

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Computer-based instructors, just like their human counterparts, should monitor the emotional and cognitive states of their students in order to adapt instructional technique. Doing so requires a model of student state to be available at run time, but this has historically been difficult. Because people are different, generalized models have not been able to be validated. As a person's cognitive and affective state vary over time of day and seasonally, individualized models have had differing difficulties. The simultaneous creation and execution of an individualized model, in real time, rep
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Shah, Niksha Chimanlal Meghji. "Construction and development of bioluminescent Pseudomonas aeruginosa strains : application in biosensors for preservative efficacy testing." Thesis, University of Hertfordshire, 2014. http://hdl.handle.net/2299/15592.

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Whole cell biosensors have been extensively used for monitoring toxicity and contamination of compounds in environmental biology and microbial ecology. However, their application in the pharmaceutical and cosmetics industries for preservative efficacy testing (PET) has been limited. According to several pharmacopoeias, preservatives should be tested for microbial activity using traditional viable count techniques; the use of whole cell microbial biosensors potentially provides an alternative, fast, and efficient method. The aim of the study was to construct and develop whole cell microbial bio
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