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Journal articles on the topic 'Real time Q-PCR'

Author: Grafiati
Published: 2 June 2025
Last updated: 31 July 2025

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1

Crow, Robert M., Jill S. Gartland, Angela T. McHugh, and Kevan M. A. Gartland. "Real-time GUS analysis using Q-PCR instrumentation." Journal of Biotechnology 126, no. 2 (2006): 135–39. http://dx.doi.org/10.1016/j.jbiotec.2006.04.018.

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2

Zhang, Wenju, Yulei Zhao, Qingjin Xu, and Qin Chen. "Development of a triplex real-time PCR for simultaneous detection of allergenic ingredients in processed food." Czech Journal of Food Sciences 36, No. 1 (2018): 22–27. http://dx.doi.org/10.17221/28/2017-cjfs.

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SYBR Green real-time or quantitative PCR (Q-PCR) is a suitable system in which to establish a multiplex method to detect allergenic ingredients in food. In this study, a triplex Q-PCR method was developed to detect trace amounts of peanut, soybean and sesame in processed food. Specific PCR primer sets were designed and the concentration of the primers used in the triplex PCR was optimised. The triplex method showed high specificity and sensitivity which were similar to those of the simplex method, and it was applied for the detection of allergenic ingredients in commercially available processe
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3

Bohrer, C. L., X. Tao, E. L. Torpey, D. Taylor, R. T. Scott, and N. R. Treff. "Detection of contamination by quantitative real-time (q)PCR." Fertility and Sterility 100, no. 3 (2013): S206. http://dx.doi.org/10.1016/j.fertnstert.2013.07.1375.

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4

Simon, P. "Q-Gene: processing quantitative real-time RT-PCR data." Bioinformatics 19, no. 11 (2003): 1439–40. http://dx.doi.org/10.1093/bioinformatics/btg157.

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5

Gallagher, Robert E. "Prime-time for real-time Q-RT-PCR in good-prognosis AML?" Blood 102, no. 8 (2003): 2709–10. http://dx.doi.org/10.1182/blood-2003-08-2660.

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6

Schneeberger, Peter M., Mirjam H. A. Hermans, Erik J. van Hannen, Jeroen J. A. Schellekens, Alexander C. A. P. Leenders, and Peter C. Wever. "Real-Time PCR with Serum Samples Is Indispensable for Early Diagnosis of Acute Q Fever." Clinical and Vaccine Immunology 17, no. 2 (2009): 286–90. http://dx.doi.org/10.1128/cvi.00454-09.

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ABSTRACT The world's largest Q fever outbreak is ongoing in The Netherlands with around 3,000 confirmed cases since the first half of 2007. Increased awareness has resulted in early referral of patients for diagnostics. An important drawback to serological diagnosis of acute Q fever is the lag phase in antibody response. Therefore, we evaluated the performance of a real-time PCR for detection of Coxiella burnetii DNA using serum samples from patients with acute Q fever. PCR, targeting IS1111, was retrospectively performed on acute-phase and follow-up convalescent-phase serum samples from 65 pa
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7

Khan, Izhar U. H., Alyssa Loughborough, and Thomas A. Edge. "DNA-based real-time detection and quantification of aeromonads from fresh water beaches on Lake Ontario." Journal of Water and Health 7, no. 2 (2009): 312–23. http://dx.doi.org/10.2166/wh.2009.041.

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The present study was designed to develop a novel, rapid, direct DNA-based protocol to enumerate aeromonads in recreational waters. An Aeromonas genus-specific real-time quantitative polymerase chain reaction (Q-PCR) protocol was developed and optimized using newly designed genus-specific oligonucleotide primers derived from the gyrase B subunit (GyrB) gene. A standard curve was developed based on the PCR protocol with a minimum quantification limit of 10 cell equivalents ml−1 achieved using an autoclaved water sample from recreational water spiked with known quantities of an Aeromonas ATCC st
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Albuquerque, Yvana Maria Maia de, Ana Luiza Magalhães de Andrade Lima, Ana Kelly Lins, Marcelo Magalhães, and Vera Magalhães. "QUANTITATIVE REAL-TIME PCR (Q-PCR) FOR SPUTUM SMEAR DIAGNOSIS OF PULMONARY TUBERCULOSIS AMONG PEOPLE WITH HIV/AIDS." Revista do Instituto de Medicina Tropical de São Paulo 56, no. 2 (2014): 139–42. http://dx.doi.org/10.1590/s0036-46652014000200009.

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Objective: To assess quantitative real-time polymerase chain reaction (q-PCR) for the sputum smear diagnosis of pulmonary tuberculosis (PTB) in patients living with HIV/AIDS with a clinical suspicion of PTB.Method: This is a prospective study to assess the accuracy of a diagnostic test, conducted on 140 sputum specimens from 140 patients living with HIV/AIDS with a clinical suspicion of PTB, attended at two referral hospitals for people living with HIV/AIDS in the city of Recife, Pernambuco, Brazil. A Löwenstein-Jensen medium culture and 7H9 broth were used as gold standard.Results: Of the 140
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9

Rodr�guez-L�zaro, David, Deborah A. Lewis, Alain A. Ocampo-Sosa, et al. "Internally Controlled Real-Time PCR Method for Quantitative Species-Specific Detection and vapA Genotyping of Rhodococcus equi." Applied and Environmental Microbiology 72, no. 6 (2006): 4256–63. http://dx.doi.org/10.1128/aem.02706-05.

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ABSTRACT We developed a novel quantitative real-time PCR (Q-PCR) method for the soil actinomycete Rhodococcus equi, an important horse pathogen and emerging human pathogen. Species-specific quantification was achieved by targeting the chromosomal monocopy gene choE, universally conserved in R. equi. The choE Q-PCR included an internal amplification control (IAC) for identification of false negatives. A second Q-PCR targeted the virulence plasmid gene vapA, carried by most horse isolates but infrequently found in isolates from other sources. The choE-IAC and vapA assays were 100% sensitive and
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10

Abbas, Adeeb, Majeed A. Sabbah, Abdul-salam Hatam, Luma A. Yasser, and Baan Abdul-Latif. "Molecular diagnosis of Iraqi chronic myeloid leukemia patients using quantitative real-time PCR." Journal of Biotechnology Research Center 4, no. 2 (2010): 64–69. http://dx.doi.org/10.24126/jobrc.2010.4.2.125.

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hronic myeloid leukemia (CML), also known as chronic granulocytic leukemia, is a form of leukemia characterized by the increased and unregulated growth of myeloid cells in the bone marrow and the accumulation of these cells in peripheral blood. Total RNA extraction, cDNA and quantitative real-time PCR (Q-RT-PCR) were done for thirty CML Iraqi patients. Hematology tests (hemoglobin, platelets and WBCs counts) were done for the same samples in the same time. The results of this study show that some samples with normal hematology values have BCR-ABL (p210) fusion transcript with molecular analysi
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11

Song, Dual, Shine Young Kim, Son A. Jo, et al. "Performance Evaluation of Real-Q Enterovirus Quantification Kit for Enterovirus by Real-time PCR." Annals of Laboratory Medicine 30, no. 6 (2010): 624–30. http://dx.doi.org/10.3343/kjlm.2010.30.6.624.

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12

Upadhyaya, S. K., S. M. Dixit, S. Shrestha, et al. "Assessment of Detection Efficacy of Mycobacterium Tuberculosis in Sputum Samples by Real-Time PCR-Based Method." Kathmandu University Journal of Science, Engineering and Technology 9, no. 1 (2013): 181–88. http://dx.doi.org/10.3126/kuset.v9i1.63859.

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Tuberculosis (TB) is a major public health problem in Nepal and ranks as one of the most prevalent communicable diseases throughout the country. In Nepal, 45% of the total population is infected with TB, and 40,000 people get TB every year. Twenty thousand new sputum-positive cases are seen annually, with 5000-7000 people dying each year from TB. Thirty sputum samples were collected from Sukraraj Tropical and Infectious Disease Hospital, Teku, Kathmandu, Nepal, and a comparative study of Acid-fast Bacilli (AFB) test and Real-time PCR was conducted separately with the culture test, regarded as
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13

Papayiannis, L. C., J. K. Brown, N. A. Seraphides, M. Hadjistylli, N. Ioannou, and N. I. Katis. "A real-time PCR assay to differentiate the B and Q biotypes of the Bemisia tabaci complex in Cyprus." Bulletin of Entomological Research 99, no. 6 (2009): 573–82. http://dx.doi.org/10.1017/s0007485308006603.

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AbstractA real-time PCR assay based on TaqMan® technology was developed and evaluated for the rapid detection of the B and Q biotypes of Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae). A survey was conducted during 2005–2007 in order to identify the distribution and prevalence of B. tabaci biotypes in Cyprus using the real-time PCR assay. More than 700 adult whiteflies collected from 35 cultivated and weed plant species were individually haplotyped using TaqMan® PCR, and the results of the assay were validated by restriction fragment length polymorphism analysis and DNA sequencing of the
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14

Rodríguez-Lázaro, David, Maria Pla, Mariela Scortti, Héctor J. Monzó, and José A. Vázquez-Boland. "A Novel Real-Time PCR for Listeria monocytogenes That Monitors Analytical Performance via an Internal Amplification Control." Applied and Environmental Microbiology 71, no. 12 (2005): 9008–12. http://dx.doi.org/10.1128/aem.71.12.9008-9012.2005.

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ABSTRACT We describe a novel quantitative real-time (Q)-PCR assay for Listeria monocytogenes based on the coamplification of a target hly gene fragment and an internal amplification control (IAC). The IAC is a chimeric double-stranded DNA containing a fragment of the rapeseed BnACCg8 gene flanked by the hly-specific target sequences. This IAC is detected using a second TaqMan probe labeled with a different fluorophore, enabling the simultaneous monitoring of the hly and IAC signals. The hly-IAC assay had a specificity and sensitivity of 100%, as assessed using 49 L. monocytogenes isolates of d
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15

Bai, Lijun, Yi-Mo Deng, Anthony J. Dodds, Sam Milliken, John Moore, and David D. F. Ma. "A SYBR Green - Based Real Time PCR Method for Detection of Haemopoietic Chimerism in Allogeneic Stem Cell Transplant Recipients." Blood 106, no. 11 (2005): 5222. http://dx.doi.org/10.1182/blood.v106.11.5222.5222.

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Abstract Aim Detection of donor cells (chimerism) in patients post allogeneic stem cell transplantation is for monitoring engraftment, early detection of graft rejection and disease relapse. Current methods include cytogenetics, blood grouping and DNA microsatellite test. Our aim is to develop a reliable and rapid real-time quantitative PCR (Q-PCR) method using SYBR green for detecting chimerism in allogeneic haemopoietic stem cell transplant recipients. Methods Twelve specific nucleotide polymorphisms (NPs) on 11 different chromosomes (including X, Y) were selected. Specific primers and fluor
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16

Atallah, Z. K., J. Bae, S. H. Jansky, D. I. Rouse, and W. R. Stevenson. "Multiplex Real-Time Quantitative PCR to Detect and Quantify Verticillium dahliae Colonization in Potato Lines that Differ in Response to Verticillium Wilt." Phytopathology® 97, no. 7 (2007): 865–72. http://dx.doi.org/10.1094/phyto-97-7-0865.

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Potato early dying (PED), also known as Verticillium wilt, caused by Verticillium dahliae, is a seasonal yield-limiting disease of potato worldwide, and PED-resistant cultivars currently represent only a small percentage of potato production. In this study, we developed a real-time quantitative polymerase chain reaction (Q-PCR) approach to detect and quantify V. dahliae. The efficiency of the designed primer pair VertBt-F/VertBt-R, derived from the sequence of the β-tubulin gene, was greater than 95% in monoplex Q-PCR and duplex (using Plexor technology) procedures with primers PotAct-F/PotAct
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17

Hwang, Sang-Hyun, Choong-Hwan Cha, Yoo-Li Kim, Oh-Joong Kwon, and Heung-Bum Oh. "Performance Evaluation of Real-Q HBV Quantification Kit for HBV DNA by Real-Time PCR." Annals of Laboratory Medicine 26, no. 6 (2006): 442–48. http://dx.doi.org/10.3343/kjlm.2006.26.6.442.

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18

Pagani, Ilaria Stefania, Orietta Spinelli, Cristina Pirrone, et al. "Quantitative DNA Real-Time PCR Compared To mRNA and Cytogenetic Assays To Monitor Minimal Residual Disease In Chronic Myeloid Leukemia." Blood 122, no. 21 (2013): 1316. http://dx.doi.org/10.1182/blood.v122.21.1316.1316.

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Abstract Introduction Imatinib mesylate (IM) is the first line therapy against Chronic Myeloid Leukemia (CML), effectively prolonging overall survival. Because discontinuation of treatment is associated with molecular relapse, IM is required indefinitely to maintain operational cure. To evaluate the degree of response to therapy and to highlight the persistence of the disease after treatment, patients should be monitored routinely. The gold standard for diagnosing CML is the cytogenetic analysis, a direct not-sensitive method to detect Ph-positive cells. Quantitative real-time RT-PCR (qRT-PCR)
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19

Fey, Axel, Stefan Eichler, S�bastien Flavier, Richard Christen, Manfred G. H�fle, and Carlos A. Guzm�n. "Establishment of a Real-Time PCR-Based Approach for Accurate Quantification of Bacterial RNA Targets in Water, Using Salmonella as a Model Organism." Applied and Environmental Microbiology 70, no. 6 (2004): 3618–23. http://dx.doi.org/10.1128/aem.70.6.3618-3623.2004.

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ABSTRACT Quantitative PCR (Q-PCR) is a fast and efficient tool to quantify target genes. In eukaryotic cells, quantitative reverse transcription-PCR (Q-RT-PCR) is also used to quantify gene expression, with stably expressed housekeeping genes as standards. In bacteria, such stable expression of housekeeping genes does not occur, and the use of DNA standards leads to a broad underestimation. Therefore, an accurate quantification of RNA is feasible only by using appropriate RNA standards. We established and validated a Q-PCR method which enables the quantification of not only the number of copie
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20

Saikaly, Pascal E., Morton A. Barlaz, and Francis L. de los Reyes. "Development of Quantitative Real-Time PCR Assays for Detection and Quantification of Surrogate Biological Warfare Agents in Building Debris and Leachate." Applied and Environmental Microbiology 73, no. 20 (2007): 6557–65. http://dx.doi.org/10.1128/aem.00779-07.

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ABSTRACT Evaluation of the fate and transport of biological warfare (BW) agents in landfills requires the development of specific and sensitive detection assays. The objective of the current study was to develop and validate SYBR green quantitative real-time PCR (Q-PCR) assays for the specific detection and quantification of surrogate BW agents in synthetic building debris (SBD) and leachate. Bacillus atrophaeus (vegetative cells and spores) and Serratia marcescens were used as surrogates for Bacillus anthracis (anthrax) and Yersinia pestis (plague), respectively. The targets for SYBR green Q-
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21

Faedda, Roberto, and Gabriela B. Silva. "Development of a Real-Time PCR Assay for the Early Detection of the Eucalyptus Pathogen Quambalaria eucalypti." Forests 15, no. 2 (2024): 375. http://dx.doi.org/10.3390/f15020375.

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Quambalaria eucalypti is a fungal pathogen that causes leaf spot, shoot blight, and stem canker on Eucalyptus spp. Early diagnosis of the disease is difficult, although the symptoms are clear in its advanced phase. To enable a rapid and sensitive screening of asymptomatic or latently infected plant material for Q. eucalypti, a SYBR green-based real-time PCR assay targeting the partial histone-H3 region was developed. The assay demonstrated specificity for Q. eucalypti, not showing cross-reactivity with other Quambalaria species or the other eucalyptus fungal pathogens tested. The primers devel
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22

Ughetto, E., F. Gouriet, D. Raoult, and J. M. Rolain. "Three years experience of real-time PCR for the diagnosis of Q fever." Clinical Microbiology and Infection 15 (December 2009): 200–201. http://dx.doi.org/10.1111/j.1469-0691.2008.02264.x.

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23

Antovich, Zachary, Thierry Viard, Roland Russnak, et al. "P123 HPA typing with LinkSe¯q™, a real-time PCR detection system." Human Immunology 77 (September 2016): 127. http://dx.doi.org/10.1016/j.humimm.2016.07.188.

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24

Liu, Xiao Kun, and Yan Hong. "Q-priming PCR: A quantitative real-time PCR system using a self-quenched BODIPY FL-labeled primer." Analytical Biochemistry 360, no. 1 (2007): 154–56. http://dx.doi.org/10.1016/j.ab.2006.10.011.

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25

Gallagher, Robert E., Esther L. Schachter-Tokarz, Dan A. Jones, and Elihu H. Estey. "Assessment of Minimal Residual Disease (MRD) by Qualitative RT-PCR (Q-PCR) vs Real-Time Quantitative RT-PCR (RQ-PCR) in a Phase II Study of Acute Promyelocytic Leukemia (APL)." Blood 106, no. 11 (2005): 3262. http://dx.doi.org/10.1182/blood.v106.11.3262.3262.

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Abstract Assessment of MRD in APL by Q-PCR analysis for the presence or absence of PML-RARα mRNA after the completion of consolidation therapy in treatment regimens employing all-trans retinoic acid (ATRA) and chemotherapy (CT) has provided a reasonably accurate but not infallible prognostic test for disease recurrence (DR). This assessment has been made at detection sensitivity levels up to 1 in 104, which is considered to have optimal predictive value, since non-quantified lower levels of PML-RARα mRNA may not be relevant to clinical outcome. In the current study, we compared the results of
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26

Smith, Marilyn S., Richard K. Yang, Charles W. Knapp, et al. "Quantification of Tetracycline Resistance Genes in Feedlot Lagoons by Real-Time PCR." Applied and Environmental Microbiology 70, no. 12 (2004): 7372–77. http://dx.doi.org/10.1128/aem.70.12.7372-7377.2004.

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ABSTRACT A new real-time PCR method is presented that detects and quantifies three tetracycline resistance (Tcr) genes [tet(O), tet(W), and tet(Q)] in mixed microbial communities resident in feedlot lagoon wastewater. Tcr gene real-time TaqMan primer-probe sets were developed and optimized to quantify the Tcr genes present in seven different cattle feedlot lagoons, to validate the method, and to assess whether resistance gene concentrations correlate with free-tetracycline levels in lagoon waters. The method proved to be sensitive across a wide range of gene concentrations and provided consist
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27

Liu, Wei, Xiwen Jiang, Yue Liu, and Qingsong Ma. "Bioinformatics Analysis of Quantitative PCR and Reverse Transcription PCR in Detecting HCV RNA." Current Bioinformatics 14, no. 5 (2019): 400–405. http://dx.doi.org/10.2174/1574893613666180703103328.

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Objective:This research aimed to make comparisons of sensitivity and specificity between Quantitative real Time Polymerase Chain Reaction (Q-PCR) and Reverse Transcription PCR (RT-PCR) in detecting the ribonucleic acid (RNA) expression levels of Hepatitis C Virus (HCV).Methods:121 patients suffering from hepatitis C and 98 healthy participants with normal liver functions were identified. The venous blood collections were carried out, were subjected to detect the expression levels of HCV RNA via Q-PCR and RT-PCR. And then, the data obtained from these above two detection methods were compared,
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28

Panferova, Yu A., O. A. Freilikhman, N. K. Tokarevich, S. F. Karpenko, and Kh M. Galimzyanov. "COMPARISON OF DIAGNOSTIC EFFECTIVENESS OF METHODS OF DETECTION OF COXIELLA BURNETII IN BLOOD OF PATIENTS WITH Q FEVER BASED ON AMPLIFICATION OF 16S rRNA GENE FRAGMENTS (STANDARD PCR) AND groEL GENE (REALTIME PCR)." Journal of microbiology, epidemiology and immunobiology, no. 3 (June 28, 2016): 70–74. http://dx.doi.org/10.36233/0372-9311-2016-3-70-74.

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Aim. Comparison of diagnostic capabilities of 2 variants of PCR for detection of Coxiella burnetii persistence in dynamics of infectious process in patients with Q fever. Materials and methods. 110 samples of clinical material, obtained from patients with Q fever in an endemic region for this infection (Astrakhan region), were studied. The samples were studied in a standard PCR (marker - 16S rRNA gene fragment) and in real-time PCR (RT-PCR) (marker - groEL gene fragment). Results. Both markers were established to be perspective for detection of C. burnetii DNA in clinical material, and RT-PCR
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29

Balaji, Boovaraghan, Dennis B. Bucholtz, and Joseph M. Anderson. "Barley yellow dwarf virus and Cereal yellow dwarf virus Quantification by Real-Time Polymerase Chain Reaction in Resistant and Susceptible Plants." Phytopathology® 93, no. 11 (2003): 1386–92. http://dx.doi.org/10.1094/phyto.2003.93.11.1386.

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Reliable detection and quantification of barley and cereal yellow dwarf viruses (YDVs) is a critical component in managing yellow dwarf diseases in small grain cereal crops. The method currently used is enzyme-linked immunosorbent assay (ELISA), using antisera against the coat proteins that are specific for each of the various YDVs. Recently, quantitative real-time reverse-transcription polymerase chain reaction (Q-RT-PCR) has been used to detect bacterial and viral pathogens and to study gene expression. We applied this technique to detect and quantify YDVs using primers specific for Barley y
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Christensen, Nynne Meyn, Mogens Nicolaisen, Michael Hansen, and Alexander Schulz. "Distribution of Phytoplasmas in Infected Plants as Revealed by Real-Time PCR and Bioimaging." Molecular Plant-Microbe Interactions® 17, no. 11 (2004): 1175–84. http://dx.doi.org/10.1094/mpmi.2004.17.11.1175.

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Phytoplasmas are cell wall-less bacteria inhabiting the phloem and utilizing it for their spread. Infected plants often show changes in growth pattern and a reduced crop yield. A quantitative real-time polymerase chain reaction (Q-PCR) assay and a bioimaging method were developed to quantify and localize phytoplasmas in situ. According to the Q-PCR assay, phytoplasmas accumulated disproportionately in source leaves of Euphorbia pulcherrima and, to a lesser extent, in petioles of source leaves and in stems. However, phytoplasma accumulation was small or nondetectable in sink organs (roots and s
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Bonyadian, Mojtaba, Hamdollah Moshtaghi, and Hamidreza Kazemeini. "The Sensitivity of the Real-time PCR and Nested-PCR for Detection of Coxiella burnetii in Milk Samples." Entomology and Applied Science Letters 4, no. 2 (2017): 11. http://dx.doi.org/10.24896/easl2017423.

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Coxiella burnetii is the causative agent of Q-fever, a widespread zoonosis. In domestic animals infection remains either asymptomatic or presents as infertility or abortion. Clinical presentation in humans can range from mild flu-like illness to acute pneumonia and hepatitis. In humans serology is the gold standard for diagnosis but is inadequate for early case detection, so real-time PCR and nested-PCR assays were developed in this study to measure amounts of C.burnetii shed in milk. Our study was to assess the sensitivity of the realtime PCR and nested-PCR for detection of Coxiella burnetii
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Stram, Yehuda, Larisa Kuznetzova, Aviad Levin, Hagai Yadin, and Marisol Rubinstein-Giuni. "A real-time RT-quantative(q)PCR for the detection of bovine ephemeral fever virus." Journal of Virological Methods 130, no. 1-2 (2005): 1–6. http://dx.doi.org/10.1016/j.jviromet.2005.05.024.

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Moniot, Maxime, Céline Nourrisson, Virginie Bonnin, et al. "Evaluation of the Bio-Evolution Microsporidia generic and typing real-time PCR assays for the diagnosis of intestinal microsporidiosis." Parasite 29 (2022): 55. http://dx.doi.org/10.1051/parasite/2022055.

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Cases of intestinal microsporidiosis infection are underestimated and affect both immunocompromized and immunocompetent patients. Real-time PCR is superseding microscopic examination for its diagnosis in medical analysis laboratories. However, few manufacturers include microsporidia in their PCR panel for the diagnosis of infectious gastroenteritis. Here, we evaluated the performances of the real-time PCR assays microsporidia generic and microsporidia typing (Bio-Evolution, France) on the Rotor-Gene Q real-time PCR cycler (Qiagen, France). We included 45 negative and 44 positive stool samples
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Babich, Bridget, George Roba, Siti Sarah Safura, Kevin Callahan, and Edward Freeman. "Transcription of nanos-1 in Zebrafish Embryos is not Affected by Bisphenol A: Evaluated Using Quantitative Real-Time PCR." American Journal of Undergraduate Research 16, no. 1 (2019): 15–21. http://dx.doi.org/10.33697/ajur.2019.012.

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The presence of primordial germ cells (PGCs) is crucial for proper gonad formation in zebrafish (Danio rerio). The many aspects of PGC migration that allow these cells to reach the proper location at the gonadal ridge include receptors, ligands, germ plasm components, and internal maintenance of PGCs. Any one of these factors could be affected by endocrine-disrupting chemicals (EDCs), which have been shown to alter the directed migration of these cells during early embryonic development. Based on recent research wherein the EDC bisphenol A (BPA) inhibited normal PGC migration, we have used the
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35

Rezasoltani, Sama, Hossein Dabiri, Hamid Asadzadeh Aghdaei, Abbas Akhavan Sepahi, Mohammad Hossein Modarressi, and Ehsan Nazemalhosseini Mojaradd. "An improved real-time qPCR technique for quantification of intestinal bacteria in human fecal samples." South Asian Journal of Experimental Biology 7, no. 5 (2018): 201–9. http://dx.doi.org/10.38150/sajeb.7(5).p201-209.

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The easy, cost-effective and rapid techniques for investigating the role of gut microbiota have become imperative owing to their importance in various diseases such as colon cancer and inflammatory bowel disease. Here we developed an absolute real-time qPCR technique for quantification of intestinal bacteria in human fecal samples for further investigation of gut microbiota composition in patients. At first, regarding bacterial species present in human fecal samples, species were cultured in anaerobic culture media and incubated at 37Cͦ in an anaerobic chamber. The number of CFU was counted.
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Senitzer, D., Y. Zhou, L. Gaidulis, and K. Lazaruk. "Chimerism Detection In Hematopoietic Cell Transplant Recipients By Real-Time Quantitative Pcr (Q-PCR) Vs. Short Tandem Repeat (STR) Analysis." Biology of Blood and Marrow Transplantation 15, no. 2 (2009): 138. http://dx.doi.org/10.1016/j.bbmt.2008.12.422.

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37

Okubara, P. A., K. L. Schroeder, and T. C. Paulitz. "Identification and Quantification of Rhizoctonia solani and R. oryzae Using Real-Time Polymerase Chain Reaction." Phytopathology® 98, no. 7 (2008): 837–47. http://dx.doi.org/10.1094/phyto-98-7-0837.

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Rhizoctonia solani and R. oryzae are the principal causal agents of Rhizoctonia root rot in dryland cereal production systems of the Pacific Northwest. To facilitate the identification and quantification of these pathogens in agricultural samples, we developed SYBR Green I-based real-time quantitative-polymerase chain reaction (Q-PCR) assays specific to internal transcribed spacers ITS1 and ITS2 of the nuclear ribosomal DNA of R. solani and R. oryzae. The assays were diagnostic for R. solani AG-2-1, AG-8, and AG-10, three genotypes of R. oryzae, and an AG-I-like binucleate Rhizoctonia species.
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AL-Hamdawee, Khetam Qaid, Noaman Naji Aaiz, and Ghasoun Mohammed-Ali Wadai. "Molecular detection of Coxiella burnetii in human and sheep in Al-Diwaniyah province by Real Time- PCR." Kufa Journal For Veterinary Medical Sciences 7, no. 2 (2016): 193–203. http://dx.doi.org/10.36326/kjvs/2016/v7i24344.

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Background: Iraq is agricultural country, but the important aspect of livestock shown by most of the studies conducted in previous years, which dealt with Q fever infection in animals ( cattle, sheep and goat ) strikingly. Q fever it is a worldwide zoonotic disease caused by Coxiella burentii .Material and methods: One hundred and five (105), human samples used in the study were (50) (22 sputum/28 bronchial-alveolar lavage) collected from Teaching Hospital and Center Pulmonology and Respiratory Specialist in Al-Diwaniyah. Collected sheep samples were (55) Sample (28 nasopharyngeal swabs and 27
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Bener, Leyla, Mustafa Ersal, and Berkant İ. Yıldız. "Genetiği Değiştirilmiş Organizmaların Tespiti ve Ölçümünde Kullanılan Farklı Gerçek Zamanlı PCR Kimyasallarının Karşılaştırılması." Turkish Journal of Agriculture - Food Science and Technology 7, sp1 (2019): 133. http://dx.doi.org/10.24925/turjaf.v7isp1.133-137.2782.

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Real-time quantitative polymerase chain reaction (q-PCR) is an advanced molecular method for determining the amount of nucleic acid in both gene expression analysis and routine Deoxyribo Nucleic Acid (DNA) measurement. An accurate measurement method is essential given that the labelling threshold for genetically modified organisms (GMO) residues in food and feed products is 5% in Japan and 0,9% in the European Union. Determination of GMO components, quantification of exact amount and determination of trace amounts in food matrices are possible in q-PCR. Various q-PCR chemicals are used for thi
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Diallo, Fatimata, Bassirou Diarra, Sadio Sarro Yeya dit, et al. "Performance of two SARS-CoV-2 rapid antigen detection tests in resource limited settings, the case of Mali." African Health Sciences 23, no. 4 (2023): 122–31. http://dx.doi.org/10.4314/ahs.v23i4.15.

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Introduction: While real-time reverse transcription PCR (RT-PCR) is the recommended laboratory method to diagnose severe acute respiratory syndrome Coronavirus 2 (SARS-CoV-2) infection, its use in resource limited settings can be difficult to maintain due to high testing demand and shortage of reagents. The aim of this study was to evaluate the performances of Realy Tech™ and Standard Q™ in comparison to RT-PCR in a relatively low COVID-19 prevalence setting, Mali.Methods: We conducted a cross-sectional study between January and April 2021 in Bamako and Kati regions to evaluate both rapid test
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Josefsen, M. H., C. L�fstr�m, T. B. Hansen, L. S. Christensen, J. E. Olsen, and J. Hoorfar. "Rapid Quantification of Viable Campylobacter Bacteria on Chicken Carcasses, Using Real-Time PCR and Propidium Monoazide Treatment, as a Tool for Quantitative Risk Assessment." Applied and Environmental Microbiology 76, no. 15 (2010): 5097–104. http://dx.doi.org/10.1128/aem.00411-10.

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ABSTRACT A number of intervention strategies against Campylobacter-contaminated poultry focus on postslaughter reduction of the number of cells, emphasizing the need for rapid and reliable quantitative detection of only viable Campylobacter bacteria. We present a new and rapid quantitative approach to the enumeration of food-borne Campylobacter bacteria that combines real-time quantitative PCR (Q-PCR) with simple propidium monoazide (PMA) sample treatment. In less than 3 h, this method generates a signal from only viable and viable but nonculturable (VBNC) Campylobacter bacteria with an intact
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42

Lauer, Wendy F., and Jean-Philippe Tourniaire. "iQ-Check Listeria spp. Real-Time PCR Test Kit." Journal of AOAC INTERNATIONAL 96, no. 3 (2013): 508–15. http://dx.doi.org/10.5740/jaoacint.govval06.

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Abstract A comparative evaluation study of the Bio-Rad® iQ-Check™Listeria species Kit (Bio-Rad Laboratories, Hercules, CA) was conducted at Q Laboratories, Inc., Cincinnati, OH. iQ-Check is a rapid method based on real-time PCR amplification and detection of all species of Listeria, including L. grayi, in food and environmental samples. The iQ-Check method was compared to the Health Canada MFHPB-30 reference method for the analysis of five ready-to-eat meats—deli turkey, hot dogs, liver paté, raw fermented sausage, and deli ham—and one stainless steel surface. Each food matrix was analyzed at
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Antovich, Zachary, Ngoc Ly, Thierry Viard, and Komal Singh. "P124 LinkSe¯q™ wipe test, a real-time PCR detection system for amplification products contamination." Human Immunology 77 (September 2016): 128. http://dx.doi.org/10.1016/j.humimm.2016.07.189.

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Harshani, Hewa Babarandage Chathurika, Denagama Vitharanage Rishan Geeth Ruwan, Udage Kankanamge Isuru Udara Samaraweera, Dedunu C. U. Dias Weligamage, and Janaki I. Abeynayake. "Advancing public health preparedness: Establishment of Nipah virus molecular diagnostic test at the National Reference Laboratory, Sri Lanka." Asian Pacific Journal of Tropical Medicine 17, no. 8 (2024): 369–74. http://dx.doi.org/10.4103/apjtm.apjtm_957_23.

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Objective: To establish Nipah virus diagnostic capabilities at the National Reference Laboratory in Sri Lanka using the NIV Pune real-time PCR kit. Methods: Strict safety precautions were adhered during testing due to the high pathogenicity of the Nipah virus, with all diagnostics conducted in a BSL2+ laboratory at the Medical Research Institute in Sri Lanka. RNA extraction was performed using the QIAamp Viral RNA Mini kit. The NIV Pune in-house real-time PCR kit was employed, following established primer/probe sequences and controls. The assay was validated using the Rotor-Gene Q Series Real-
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Chung, Yoo Na, In Young Yoo, Sun Ae Yun, Ji-Youn Kim, Nam Yong Lee, and Hee Jae Huh. "Comparison of the AdvanSure RV Plus Real-Time RT-PCR and Real-Q RV II Detection Assays for Respiratory Viruses." Annals of Laboratory Medicine 41, no. 5 (2021): 506–9. http://dx.doi.org/10.3343/alm.2021.41.5.506.

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46

Chakrabartty, A., P. K. Bhattacharjee, R. R. Sarker, et al. "PREVALENCE OF COXIELLA BURNETII INFECTION IN CATTLE, BLACK BENGAL GOATS AND TICKS IN BANGLADESH." Bangladesh Journal of Veterinary Medicine 14, no. 1 (2016): 65–68. http://dx.doi.org/10.3329/bjvm.v14i1.28827.

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The objectives of this study were to determine the prevalence of Coxiella burnetii infection in domestic ruminants and to detect Coxiella burnetii DNA from ticks and serum samples. A total of 24 ticks, 91 goats and 81 cattle serum samples with the history of abortion and reproductive disorders were collected from the different areas in Bangladesh. The serum samples were tested by CHEKIT Q-Fever Antibody ELISA Test Kit and Coxiella burnetii DNA was detected by multiplex quantitative real- time PCR. The overall prevalence was 7.6% and 6.1% in goats and cattle, respectively. However, none of sero
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Zheng, Ling-Jie, Ning Liu, Kun Yang, Ai-Feng Wang, Zhi-Rong Tan, and Xiang Li. "Clinical application and importance of one-step human CYP2C19 genotype detection." Journal of International Medical Research 46, no. 12 (2018): 4965–73. http://dx.doi.org/10.1177/0300060518787718.

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Background To directly achieve cytochrome P450 2C19 gene ( CYP2C19) classification using one-step real-time fluorescent PCR detection and to verify the capabilities of this method with nucleic acid extracted from whole blood samples. Methods A human CYP2C19 genotyping kit based on one-step real-time fluorescent PCR detection was used to analyze whole blood or genomic DNA samples. This method was compared with pyrosequencing and another quantitative (q)PCR kit for its accuracy, repeatability, detection range analysis, sensitivity, specificity, and anti-interference analysis. Results The one-ste
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Rahman, Md Arifur, Md Mahbub Alam, Md Aminul Islam, A. K. Fazlul Haque Bhuiyan, and A. K. M. Anisur Rahman. "Serological and Molecular Evidence of Q Fever in Domestic Ruminants in Bangladesh." Veterinary Medicine International 2016 (2016): 1–7. http://dx.doi.org/10.1155/2016/9098416.

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The objective of this study was to know the herd and animal level prevalence of Q fever in domestic ruminants in some selected districts in Bangladesh. Randomly collected 111 bulk milk and 94 sera samples of cattle, sheep, and goats were tested by indirect ELISA (iELISA). DNA extracted from 23 aborted fetal membranes was analyzed by real time (rt) PCR. The positive cut-off value of iELISA in bulk milk and individual animal sera was ≥30% and ≥40%, respectively. The overall herd level prevalence of Q fever in dairy cattle was 15.6%. The prevalence of Q fever in dairy cattle was significantly hig
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Hu, H., M. J. Davis, and R. H. Brlansky. "Quantification of Live ‘Candidatus Liberibacter asiaticus’ Populations Using Real-Time PCR and Propidium Monoazide." Plant Disease 97, no. 9 (2013): 1158–67. http://dx.doi.org/10.1094/pdis-09-12-0880-re.

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Huanglongbing (HLB) is a devastating citrus disease. It is associated with a phloem-restricted bacterium, ‘Candidatus Liberibacter asiaticus’, and primarily transmitted by Asian citrus psyllid in Florida. Because Liberibacter cannot be cultured, early diagnosis of HLB relies on DNA-based polymerase chain reaction (PCR), including real-time quantitative (q)PCR. Although estimating genomes from live bacteria (GLB) is critical for HLB research, PCR does not distinguish between live and dead cells and, thus, does not estimate GLB in hosts. Propidium monoazide (PMA), a novel DNA-binding dye, has be
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Anjos, Taís Ramalho dos, Maria Júlia Sudária, Vinícius Silva Castro, Eduardo Eustáquio de Souza Figueiredo, and Ricardo César Tavares Carvalho. "The Rv2807 target gene: a determining factor to directly detect Mycobacterium bovis from suspected bovine tuberculosis lesions." Acta Veterinaria Brasilica 16, no. 4 (2022): 309–12. http://dx.doi.org/10.21708/avb.2022.16.4.10988.

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Bovine tuberculosis (bTB) is a zoonosis caused by Mycobacterium bovis, a species belonging to the Mycobacterium tuberculosis complex (MTC) group. Direct bTB diagnosis from suggestive lesions can be performed by nested q-PCR targeting the Rv2807 gene present in the MTC group, as well as the TbD1 gene, present in M. bovis. In this context, the aim of the present study was to assess the importance of considering positive MTC results for the Rv2807 target gene obtained through the nested real time polymerase chain reaction (nested q-PCR) applied to samples obtained directly from suspected bTB lesi
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