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Relevant bibliographies by topics / Real-Time quantitative PCRs

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Academic literature on the topic 'Real-Time quantitative PCRs'

Author: Grafiati

Published: 11 May 2023

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Journal articles on the topic "Real-Time quantitative PCRs"

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Blanda, Valeria, Rosalia D’Agostino, Elisabetta Giudice, et al. "New Real-Time PCRs to Differentiate Rickettsia spp. and Rickettsia conorii." Molecules 25, no. 19 (2020): 4431. http://dx.doi.org/10.3390/molecules25194431.

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Rickettsia species are an important cause of emerging infectious diseases in people and animals, and rickettsiosis is one of the oldest known vector-borne diseases. Laboratory diagnosis of Rickettsia is complex and time-consuming. This study was aimed at developing two quantitative real-time PCRs targeting ompB and ompA genes for the detection, respectively, of Rickettsia spp. and R. conorii DNA. Primers were designed following an analysis of Rickettsia gene sequences. The assays were optimized using SYBR Green and TaqMan methods and tested for sensitivity and specificity. This study allowed t

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Joly, Philippe, Pierre-Alain Falconnet, Janine André, et al. "Quantitative Real-Time Legionella PCR for Environmental Water Samples: Data Interpretation." Applied and Environmental Microbiology 72, no. 4 (2006): 2801–8. http://dx.doi.org/10.1128/aem.72.4.2801-2808.2006.

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ABSTRACT Quantitative Legionella PCRs targeting the 16S rRNA gene (specific for the genus Legionella) and the mip gene (specific for the species Legionella pneumophila) were applied to a total of 223 hot water system samples (131 in one laboratory and 92 in another laboratory) and 37 cooling tower samples (all in the same laboratory). The PCR results were compared with those of conventional culture. 16S rRNA gene PCR results were nonquantifiable for 2.8% of cooling tower samples and up to 39.1% of hot water system samples, and this was highly predictive of Legionella CFU counts below 250/liter

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Ruszova, E., M. Chmelarova, M. Senkerikova, and S. Stefackova. "Duplex Methylation-Specific Semi-Quantitative Real-Time PCR for Cost-Effective & Time-Efficient Diagnostic Screening of Chromosome 15 and 14 Imprinted Regions." Acta Medica Martiniana 15, no. 3 (2015): 5–12. http://dx.doi.org/10.1515/acm-2015-0012.

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AbstractPurpose: Our goal was to develop two-tier strategy based onin house-designed methylation specific-duplex polymerase chain reactions (MS-PCRs) that could serve as a relatively simple, cost effective, time efficient approach for molecular screening of imprinted regions on chromosomes 15 and 14.Patients and methods: Patients were referred to examination during infancy due to hypotonia and motor development delay. Duplex MS-PCRs were performed that enabled detection of methylated/unmethylated DNA inNDNandMEG3CpG islands via plurality of detection channels on PCR instrument Rotor Gene 6000.

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Papp, Stefanie, Jessica Rauch, Svenja Kuehl, Ulricke Richardt, Christian Keller, and Anke Osterloh. "Comparative evaluation of two Rickettsia typhi-specific quantitative real-time PCRs for research and diagnostic purposes." Medical Microbiology and Immunology 206, no. 1 (2016): 41–51. http://dx.doi.org/10.1007/s00430-016-0480-z.

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Lopotova, Tereza, Jana Moravcova, Vaclava Polivkova, et al. "Expression of Four Major WT1 Splicing Variants in AML and CML: Development of Quantitative Real-Time PCRs and Preliminary Results in Patient Samples." Blood 114, no. 22 (2009): 1631. http://dx.doi.org/10.1182/blood.v114.22.1631.1631.

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Abstract Abstract 1631 Poster Board I-657 WT1 expression has proved to be of prognostic significance in AML, MDS and according to our unpublished data also in CML patients. There are four major WT1 isoforms with distinct functions in cells. Splicing variants encoding the WT1 isoforms differ from each other by the presence or absence of both the exon 5 and KTS region in the exon 9 of WT1 gene. It was shown that exon 5 containing isoforms appear to cause cell cycle arrest. Therefore, differences in WT1 splicing variant ratios might be associated with prognosis and response to therapy. All curren

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Maksimov, Pavlo, Hannes Bergmann, Marion Wassermann, et al. "Species Detection within the Echinococcus granulosus sensu lato Complex by Novel Probe-Based Real-Time PCRs." Pathogens 9, no. 10 (2020): 791. http://dx.doi.org/10.3390/pathogens9100791.

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Infections with eggs of Echinococcus granulosus sensu lato (s.l.) can cause cystic echinococcosis in intermediate host animals and humans. Upon ingestion of viable eggs, oncospheres hatch from the eggs and subsequently develop into fluid-filled larval cysts, most frequently in the liver or the lungs. The slowly growing cysts progressively interfere with organ function. The risk of infection is determined by the host range of the parasite, its pathogenicity and other epidemiologically relevant parameters, which differ significantly among the five species within the E. granulosus s.l. complex. I

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Wall, Steven J., and Dylan R. Edwards. "Quantitative Reverse Transcription–Polymerase Chain Reaction (RT-PCR): A Comparison of Primer-Dropping, Competitive, and Real-Time RT-PCRs." Analytical Biochemistry 300, no. 2 (2002): 269–73. http://dx.doi.org/10.1006/abio.2001.5458.

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Gautam, Rashi, Slavica Mijatovic-Rustempasic, Mathew D. Esona, Ka Ian Tam, Osbourne Quaye, and Michael D. Bowen. "One-step multiplex real-time RT-PCR assay for detecting and genotyping wild-type group A rotavirus strains and vaccine strains (Rotarix® and RotaTeq®) in stool samples." PeerJ 4 (January 11, 2016): e1560. http://dx.doi.org/10.7717/peerj.1560.

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Background.Group A rotavirus (RVA) infection is the major cause of acute gastroenteritis (AGE) in young children worldwide. Introduction of two live-attenuated rotavirus vaccines, RotaTeq® and Rotarix®, has dramatically reduced RVA associated AGE and mortality in developed as well as in many developing countries. High-throughput methods are needed to genotype rotavirus wild-type strains and to identify vaccine strains in stool samples. Quantitative RT-PCR assays (qRT-PCR) offer several advantages including increased sensitivity, higher throughput, and faster turnaround time.Methods.In this stu

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Wen, Jing, Hong Yang, Kongjia Luo, et al. "A three-gene expression signature model to predict neo-chemoradiotherapy response of esophageal squamous cell carcinomas." Journal of Clinical Oncology 31, no. 15_suppl (2013): e15135-e15135. http://dx.doi.org/10.1200/jco.2013.31.15_suppl.e15135.

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e15135 Background: Preoperative chemoradiotherapy (CRT) followed by surgery has been proved to improve survival in comparison with surgery alone. However, the outcomes of CRT are heterogeneous, and no clinical or pathological method could prediction CRT response. In this study, we aim to identify mRNA markers for ESCC CRT-response prediction. Methods: Gene expression analyses were performed on pretreatment cancer biopsies from 28 ESCCs who received neoadjuvant CRT and surgery. Surgical specimens were assessed for the pathological response to CRT. The identified differentially expressed genes w

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Skubic, Lucijan, Lea Hošnjak, Vesna Breznik, Kristina Fujs Komloš, Boštjan Luzar, and Mario Poljak. "An Improved Protocol for Comprehensive Etiological Characterization of Skin Warts and Determining Causative Human Papillomavirus Types in 128 Histologically Confirmed Common Warts." Viruses 14, no. 10 (2022): 2266. http://dx.doi.org/10.3390/v14102266.

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Human papillomaviruses (HPVs) are etiologically associated with various benign and malignant neoplasms of cutaneous and mucosal epithelia. We describe an improved diagnostic protocol for comprehensive characterization of causative HPV types in common warts, in which broad-spectrum PCRs followed by Sanger sequencing, two previously described and seven newly developed type-specific quantitative real-time PCRs (qPCRs) coupled with the human beta-globin qPCR were used for: (i) diagnosis of HPV infection in warts; (ii) estimation of cellular viral loads of all HPV types detected; and (iii) determin

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Dissertations / Theses on the topic "Real-Time quantitative PCRs"

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Desvars, Amélie. "Épidémiologie d'une zoonose, la leptospirose, dans deux îles de l'océan Indien, la Réunion et Mayotte - Étude comparée du rôle de différentes espèces sauvages et domestiques." Electronic Thesis or Diss., La Réunion, 2012. http://www.theses.fr/2012LARE0039.

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La leptospirose est une zoonose de répartition mondiale dont les formes graves peuvent être mortelles pour l'Homme. Tous les mammifères sont susceptibles d'être réservoirs, la connaissance des hôtes réservoirs de Leptospira est essentielle à la mise en place de mesures de prévention. L'objectif de ce travail était de conduire une étude épidémiologique descriptive transversale de la leptospirose animale dans deux îles de l'Océan Indien : La Réunion et Mayotte. À La Réunion, 579 animaux appartenant à 13 espèces ont été prélevés. Nous montrons que la maladie circule chez l'ensemble des espèces, s

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Zhang, Yan. "Frequent RASSF1A gene promoter hypermethylation in breast cancer." [S.l. : s.n.], 2008. http://nbn-resolving.de/urn:nbn:de:bsz:289-vts-63611.

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Andalo, Alice. "Analisi quantitativa dell'espressione genica mediante real-time rt-pcr." Bachelor's thesis, Alma Mater Studiorum - Università di Bologna, 2015. http://amslaurea.unibo.it/8450/.

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Il primo capitolo di questo lavoro di tesi introduce i concetti di biologia necessari per comprendere il fenomeno dell’espressione genica. Il secondo capitolo descrive i metodi e le tecniche di laboratorio utilizzate per ottenere il cDNA, il materiale genetico che verrà amplificato nella real-time PCR. Nel terzo capitolo si descrive la tecnica di real-time PCR, partendo da una descrizione della PCR convenzionale fino a delineare le caratteristiche della sua evoluzione in real-time PCR. Si prosegue con la spiegazione del principio fisico alla base della tecnica e delle molecole necessarie (fluo

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Tichopád, Aleš. "Quantitative real-time RT-PCR based transcriptomics improvement of evaluation methods /." [S.l.] : [s.n.], 2004. http://deposit.ddb.de/cgi-bin/dokserv?idn=972765069.

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Steyn, HC, A. Pretorius, and CME McCrindle. "A quantitative real-time PCR assay for Ehrlichia ruminantium using pCS20." Elsevier, 2008. http://encore.tut.ac.za/iii/cpro/DigitalItemViewPage.external?sp=1000379.

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Heartwater is a tick borne disease that affects ruminants and wild animals in Africa south of the Sahara. It is caused by Ehrlichia ruminantium and transmitted by the tick Amblyomma hebraeum. The protocols currently used to detect heartwater take several days to complete. Here, we describe the development of a pCS20 quantitative real-time PCRTaqMan probe assay to detect E. ruminantium in livestock blood and ticks from the field. The assay is based on the conserved pCS20 gene region of E. ruminantium that contains two overlapping genes, rnc and ctaG [Collins, N.E., Liebenberg, J., De Villiers,

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Leopold, Luciana Eleanor Dittmer Dirk Peter. "Development of real-time PCR assays for the quantitative detection of herpesviruses." Chapel Hill, N.C. : University of North Carolina at Chapel Hill, 2008. http://dc.lib.unc.edu/u?/etd,1487.

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Thesis (M.S.)--University of North Carolina at Chapel Hill, 2008.<br>Title from electronic title page (viewed Sep. 16, 2008). "... in partial fulfillment of the requirements for the degree of Master of Science in the Curriculum of Genetics and Molecular Biology." Discipline: Genetics and Molecular Biology; Department/School: Medicine.

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Jungebloud, Anke. "Untersuchung der Genexpression in Aspergillus niger mittels Echtzeit-PCR." Paderborn FIT-Verl. für Innovation und Technologietransfer, 2007. http://www.gbv.de/dms/bs/toc/533996201.pdf.

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Rosa, Stefanie Ulrike. ""Real-Time-PCR-Untersuchungen zur Persistenz von infektiösen Toxoplasma-gondii-Dauerstadien in Rohwurst-Erzeugnissen"." Giessen VVB Laufersweiler, 2009. http://d-nb.info/996004408/04.

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Lorenz, Andreas. "Quantitative Real-time PCR zum spezifischen Nachweis transrenaler DNA des Mycobacterium tuberculosis complex." Diss., lmu, 2010. http://nbn-resolving.de/urn:nbn:de:bvb:19-115143.

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Utokaparch, Soraya. "Development of a standardized quantitative real time PCR panel for respiratory viral diagnosis." Thesis, University of British Columbia, 2006. http://hdl.handle.net/2429/31339.

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Traditional viral diagnostics such as viral culture and various serological techniques tend to be slow, insensitive and labour-intensive. A large proportion of viral pathogens still go undetected using these techniques. This thesis concerns the development of a rapid and sensitive technique - standardized real-time quantitative PCR. Individual qPCR assays and synthetic plasmid controls were developed for 12 common respiratory viruses including influenza types A and B, parainfluenza (PIV)-l, -2 and -3, respiratory syncytial virus (RSV) A and B, metapneumovirus (MPV), human coronavirus (HC

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Books on the topic "Real-Time quantitative PCRs"

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Biassoni, Roberto, and Alessandro Raso, eds. Quantitative Real-Time PCR. Springer New York, 2014. http://dx.doi.org/10.1007/978-1-4939-0733-5.

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Biassoni, Roberto, and Alessandro Raso, eds. Quantitative Real-Time PCR. Springer New York, 2020. http://dx.doi.org/10.1007/978-1-4939-9833-3.

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Quantitative real-time PCR in applied microbiology. Caister Academic Press, 2012.

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Quantitative real-time PCR: Methods and protocols. Humana Press, 2014.

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Biassoni, Roberto, and Alessandro Raso. Quantitative Real-Time PCR: Methods and Protocols. Springer New York, 2016.

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Biassoni, Roberto, and Alessandro Raso. Quantitative Real-Time PCR: Methods and Protocols. Springer New York, 2019.

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Biassoni, Roberto, and Alessandro Raso. Quantitative Real-Time PCR: Methods and Protocols. Springer New York, 2020.

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Taberlet, Pierre, Aurélie Bonin, Lucie Zinger, and Eric Coissac. Single-species detection. Oxford University Press, 2018. http://dx.doi.org/10.1093/oso/9780198767220.003.0009.

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Chapter 9 “Single-species detection” deals with the practical aspects of detecting a single and predefined taxon with eDNA, with a particular focus on the use of quantitative PCR (qPCR) for this purpose. After presenting how single-species detection has been implemented in a few seminal studies, it details the principles underlying qPCR. More specifically, it describes the typical qPCR amplification curve and the different systems (SYBR green and TaqMan probe assays) available to record amplicon accumulation in real time via fluorescence measurements. Chapter 9 also explains how the initial nu

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Book chapters on the topic "Real-Time quantitative PCRs"

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Sluijter, J. P. G., G. Pasterkamp, and D. P. V. de Kleijn. "Quantitative Real-Time PCR." In Cardiovascular Research. Springer US, 2006. http://dx.doi.org/10.1007/0-387-23329-6_4.

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Broll, Hermann. "Quantitative Real-Time PCR." In Molecular Biological and Immunological Techniques and Applications for Food Chemists. John Wiley & Sons, Inc., 2010. http://dx.doi.org/10.1002/9780470637685.ch3.

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Dotti, Isabella, Ermanno Nardon, Danae Pracella, and Serena Bonin. "Quantitative Real-Time RT-PCR." In Guidelines for Molecular Analysis in Archive Tissues. Springer Berlin Heidelberg, 2011. http://dx.doi.org/10.1007/978-3-642-17890-0_25.

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Martinez, Jeanelle M., and Nigel J. Walker. "Real-Time and Quantitative PCR." In Toxicogenomics. John Wiley & Sons, Inc., 2005. http://dx.doi.org/10.1002/0471669040.ch7.

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Dötsch, Jörg, Ellen Schoof, and Wolfgang Rascher. "Quantitative TaqMan Real-Time PCR." In Medical Biomethods Handbook. Humana Press, 2005. http://dx.doi.org/10.1385/1-59259-870-6:305.

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Pal, Aruna. "Real-Time or Quantitative PCR." In Springer Protocols Handbooks. Springer US, 2021. http://dx.doi.org/10.1007/978-1-0716-1818-9_9.

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Singh, Charanjeet, and Sinchita Roy-Chowdhuri. "Quantitative Real-Time PCR: Recent Advances." In Clinical Applications of PCR. Springer New York, 2016. http://dx.doi.org/10.1007/978-1-4939-3360-0_15.

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Honda, Junichi, and Kotaro Oizumi. "Quantitative Analysis of CMV in Infected Mice on the LightCycler System." In Rapid Cycle Real-Time PCR. Springer Berlin Heidelberg, 2001. http://dx.doi.org/10.1007/978-3-642-59524-0_37.

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Krüger, Petra, Albrecht Wiedenmann, Despina Tougianidou, and Konrad Botzenhart. "Quantitative Detection of Cryptosporidium parvum after In Vitro Excystation by LightCycler PCR." In Rapid Cycle Real-Time PCR. Springer Berlin Heidelberg, 2001. http://dx.doi.org/10.1007/978-3-642-59524-0_36.

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Caplin, Brian Erich. "Quantification of Human Papilloma Virus Type 16 Using Quantitative Competitive PCR on the LightCycler." In Rapid Cycle Real-Time PCR. Springer Berlin Heidelberg, 2001. http://dx.doi.org/10.1007/978-3-642-59524-0_6.

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Conference papers on the topic "Real-Time quantitative PCRs"

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Woudenberg, Timothy M., and J. Stevens. "Quantitative PCR by real-time detection." In Photonics West '96, edited by Gerald E. Cohn, Steven A. Soper, and C. H. Winston Chen. SPIE, 1996. http://dx.doi.org/10.1117/12.237619.

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"RqPCRAnalysis: Analysis of Quantitative Real-time PCR Data." In International Conference on Bioinformatics Models, Methods and Algorithms. SciTePress - Science and and Technology Publications, 2013. http://dx.doi.org/10.5220/0004312002020211.

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Sayers, Michael B., and Tara M. Dalton. "A Real-Time Continuous Flow Polymerase Chain Reactor for DNA Expression Quantification." In ASME 2007 International Mechanical Engineering Congress and Exposition. ASMEDC, 2007. http://dx.doi.org/10.1115/imece2007-43058.

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Real-time quantitative Polymerase Chain Reaction (PCR) is an extremely sensitive and reliable method for quantifying gene expression, allowing subtle shifts in gene expression to be easily monitored. Currently, stationary real-time PCR is readily achieved using fluorescent labels which increase in fluorescence as the DNA is exponentially amplified. Quantitative PCR is used in a myriad of applications. However currently most commercial real-time PCR devices are batch process stationary well based systems, limiting their throughput. Continuous flow microfluidic PCR devices have allowed for advan

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Ma, Yutong, Liang Zeng, and Jianhuan Zhang. "A fluorescence detection optical system for real-time quantitative PCR." In Optical Design and Testing X, edited by Rengmao Wu, Osamu Matoba, Yongtian Wang, and Tina E. Kidger. SPIE, 2020. http://dx.doi.org/10.1117/12.2574901.

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"Quantitative real-time PCR as a supplementary tool for molecular cytogenetics." In Plant Genetics, Genomics, Bioinformatics, and Biotechnology. Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, 2019. http://dx.doi.org/10.18699/plantgen2019-044.

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"Detection of Gene Expression from Vitis by Real Time Quantitative RT-PCR." In 2018 4th World Conference on Control, Electronics and Computer Engineering. Francis Academic Press, 2018. http://dx.doi.org/10.25236/wccece.2018.15.

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Huai, Yahong, and Shangzhong Xu. "Real-time Fluorescence Quantitative PCR and Prediction of Bovine Trf 2 Protein Funtion." In International Conference on Biomedical and Biological Engineering. Atlantis Press, 2016. http://dx.doi.org/10.2991/bbe-16.2016.30.

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Daly, John, and Mark Davies. "A Quantitative Free Convection DNA Amplifier." In ASME/JSME 2007 Thermal Engineering Heat Transfer Summer Conference collocated with the ASME 2007 InterPACK Conference. ASMEDC, 2007. http://dx.doi.org/10.1115/ht2007-32381.

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The Polymerase Chain Reaction (PCR) has been used extensively to amplify targeted nucleic acids for many applications in molecular biology and, increasingly, in medical diagnostics. Outlined in this paper is a PCR device which takes account of the advantages offered by free convection. The design is, in it fundamental format a time-wise isothermal well-based thermocycler. A temperature gradient induced across the well causes convection forces to circulate the sample through the required temperatures necessary for amplification. Quantitative amplification is demonstrated with real time measurem

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Taha, Aza, Katan Ali, and Furat Sabeer. "Quantitation assay of hepatitis C virus RNA using real-time PCR technique." In 4th International Scientific Conference of Cihan University-Erbil on Biological Sciences. Cihan University-Erbil, 2017. http://dx.doi.org/10.24086/bios17.22.

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Botteldoorn, N., H. Werbrouck, N. Rijpens, E. van Coillie, M. Heyndrickx, and L. Herman. "Expression study by real-time quantitative RT-PCR of the Salmonella typhimurium mntH gene." In Second International Symposium on Epidemiology and Control of Salmonella in Pork. Iowa State University, Digital Press, 2003. http://dx.doi.org/10.31274/safepork-180809-463.

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Reports on the topic "Real-Time quantitative PCRs"

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Harris, D. L. Hank, Isabel Turney Harris, James S. Dickson, Stephen Gaul, Brad T. Bosworth, and Lori Feldmann. Quantitative Real-time PCR (qPCR) for the Determination of Salmonella Levels in Lairage. Iowa State University, 2009. http://dx.doi.org/10.31274/ans_air-180814-1009.

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Sykes, Mark, Rati Bell, Joseph Holland, Kate Perkins, and Joy Kaye. Inter-laboratory collaborative trial of real-time PCR method for the relative quantitation of horse DNA and pork DNA in raw and processed beef DNA phases 1 and 2. Food Standards Agency, 2023. http://dx.doi.org/10.46756/sci.fsa.qbu570.

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The project describes the full international interlaboratory collaborative trial to define the performance characteristics of the real-time PCR method for horse and pork DNA in raw and processed beef matrix.

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Zhelev, Doncho V., Christopher Dupuis, Suelynn Ren, Anna Le, Mia Hunt, and Henry Gibbons. Single Nucleotide Polymorphisms (SNP)-specific Quantitative Real Time Polymerase Chain Reaction (PCR) Assay for Analyzing Competition and Emergence of the Military Hypersporulating Strains of Bacillus Atrophaeous var. Globigii. Defense Technical Information Center, 2012. http://dx.doi.org/10.21236/ada570597.

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Seroussi, Eyal, and George Liu. Genome-Wide Association Study of Copy Number Variation and QTL for Economic Traits in Holstein Cattle. United States Department of Agriculture, 2010. http://dx.doi.org/10.32747/2010.7593397.bard.

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Copy number variation (CNV) has been recently identified in human and other mammalian genomes and increasing awareness that CNV might be a major source for heritable variation in complex traits has emerged. Despite this, little has been published on CNVs in Holsteins. In order to fill this knowledge-gap, we proposed a genome-wide association study between quantitative trait loci (QTL) for economic traits and CNV in the Holstein cattle. The approved feasibility study was aimed at the genome-wide characterization of CNVs in Holstein cattle and at the demonstrating of their possible association w

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Ghanim, Murad, Joe Cicero, Judith K. Brown, and Henryk Czosnek. Dissection of Whitefly-geminivirus Interactions at the Transcriptomic, Proteomic and Cellular Levels. United States Department of Agriculture, 2010. http://dx.doi.org/10.32747/2010.7592654.bard.

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Our project focuses on gene expression and proteomics of the whitefly Bemisia tabaci (Gennadius) species complex in relation to the internal anatomy and localization of expressed genes and virions in the whitefly vector, which poses a major constraint to vegetable and fiber production in Israel and the USA. While many biological parameters are known for begomovirus transmission, nothing is known about vector proteins involved in the specific interactions between begomoviruses and their whitefly vectors. Identifying such proteins is expected to lead to the design of novel control methods that i

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