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1

Grellert, Mateus, Bruno Zatt, Muhammad Shafique, Sergio Bampi, and Jörg Henkel. "Complexity control of HEVC encoders targeting real-time constraints." Journal of Real-Time Image Processing 13, no. 1 (2016): 5–24. http://dx.doi.org/10.1007/s11554-016-0602-2.

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2

Masoom, Seyedeh Nina, Karthik M. Sundaram, Pejman Ghanouni, et al. "Real-Time MRI-Guided Prostate Interventions." Cancers 14, no. 8 (2022): 1860. http://dx.doi.org/10.3390/cancers14081860.

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Prostate cancer (PCa) is the second most common cause of cancer death in males. Targeting MRI-visible lesions has led to an overall increase in the detection of clinically significant PCa compared to the prior practice of random ultrasound-guided biopsy of the prostate. Additionally, advances in MRI-guided minimally invasive focal treatments are providing new options for patients with PCa. This review summarizes the currently utilized real-time MRI-guided interventions for PCa diagnosis and treatment.
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Wang, Jun, Weinan Zhang, and Shuai Yuan. "Display Advertising with Real-Time Bidding (RTB) and Behavioural Targeting." Foundations and Trends® in Information Retrieval 11, no. 4-5 (2017): 297–435. http://dx.doi.org/10.1561/1500000049.

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4

Lindh, Magnus, Staffan Görander, Elin Andersson, Peter Horal, Inger Mattsby-Balzer, and Walter Ryd. "Real-time Taqman PCR targeting 14 human papilloma virus types." Journal of Clinical Virology 40, no. 4 (2007): 321–24. http://dx.doi.org/10.1016/j.jcv.2007.09.009.

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5

Needle, Coby L., and Rui Catarino. "Evaluating the effect of real-time closures on cod targeting." ICES Journal of Marine Science 68, no. 8 (2011): 1647–55. http://dx.doi.org/10.1093/icesjms/fsr092.

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Abstract Needle, C. L., and Catarino, R. 2011. Evaluating the effect of real-time closures on cod targeting. – ICES Journal of Marine Science, 68: 1647–1655. Under its Conservation Credits scheme to reduce cod mortality, the Scottish Government has implemented a system of real-time closures (RTCs) since 2008. These are relatively small, temporarily closed areas (50–225 square nautical miles per RTC, closed for 21 d) that are triggered by high cod catches. An important step in evaluating their effectiveness is to determine the response of vessels to RTCs, because the conservation benefit would be reduced if vessels moved to areas of greater cod abundance following closures. Abundance indices from research-vessel surveys and commercial-vessel observer trips are combined to create a time- and space-dependent relative cod-importance index (RCII). Vessel monitoring system data from Scottish vessels fishing during 2008/2009 are used to construct RCII profiles for each vessel, which are then used to determine whether the areas to which vessels move have a higher or a lower RCII, and how far away they move when an RTC is activated. We show that the RCII of the areas moved to tends to be lower than that of the RTC and that vessels travel farther when moving away from a closure than when moving back after reopening. Although not conclusive, this result indicates that RTCs may impact beneficially on cod mortality.
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Selim, A., M. El-Haig, and E. S. Galila. "Direct detection of Mycobacterium avium SUBSP. paratuberculosis in bovine milk by multiplex real-time PCR." Biotehnologija u stocarstvu 29, no. 3 (2013): 513–25. http://dx.doi.org/10.2298/bah1303513s.

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This study aimed to direct detection of Mycobacterium avium subsp. paratuberculosis (MAP) in milk by evaluating a multiplex real-time PCR assay targeting IS900 and ISMAV2 sequences including the amplification of PUC19-plasmid as internal control. The sensitivity of the assays was evaluated by testing MAP isolates in broad linear range of DNA (50 ng - 5 fg/?l). For the validation of the specificity, 6 MAP isolates and 22 isolates of genus Mycobacteriacea were tested. Results revealed that reproducible detection limit for real-time PCR targeting IS900 and ISMAV2 was 5 fg/?l and 50 fg/?l respectively. By targeting ISMAV2 sequence, 100% specificity was detected. However, a cross reaction with 5 ng/?l of genome of 3 M. avian subspecies avium strains was detected by targeting IS900 and negative in lower genome quantity (5pg/?l). To maximize the assay?s detection sensitivity, an efficient strategy for MAP-DNA extraction from spiked milk was assessed. Targeting of IS900 was sensitive and targeting ISMAV2 was very specific. Therefore, a multiplex real-time PCR assay targeting IS900 and ISMAV2 in combination with two commercial DNA extraction kits could be an ideal sensitive and specific protocol for routine large scale analysis of milk samples and other clinical specimens from man and animals.
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Boutekkouk, Fateh. "Real-Time Embedded Systems Scheduling Optimization." International Journal of Applied Evolutionary Computation 12, no. 1 (2021): 43–73. http://dx.doi.org/10.4018/ijaec.2021010104.

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The embedded real-time scheduling problem is qualified as a hard multi-objective optimization problem under constraints since it should compromise between three key conflictual objectives that are tasks deadlines guarantee, energy consumption reduction, and reliability enhancement. On this fact, conventional approaches can easily fail to find a good tradeoff in particular when the design space is too vast. On the other side, bio-inspired meta-heuristics have proved their efficiency even if the design space is very large. In this framework, the authors review the most pertinent works of literature targeting the application of bio-inspired methods to resolve the real-time scheduling problem for embedded systems, notably artificial immune systems, machine learning, cellular automata, evolutionary algorithms, and swarm intelligence. A deep discussion is conducted putting the light on the main challenges of using bio-inspired methods in the context of embedded systems. At the end of this review, the authors highlight some of the future directions.
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8

Wu, Yajun, Yange Yang, Bin Wang, Mingchang Liu, Jianxun Han, and Ying Chen. "A Real-Time PCR Method Targeting Camel Ingredient for Food Authentication." Journal of AOAC INTERNATIONAL 98, no. 6 (2015): 1640–44. http://dx.doi.org/10.5740/jaoacint.15-155.

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Abstract The special nutritious value of camel showed high potential for market exploitation. In this paper, a real-time PCR method targeting camel ingredient in camel meat and milk is reported as an approach to fight against adulteration. To understand the impact of processing procedures on the amplifiability of cytb gene, four kinds of processed camel meat were investigated, and the rate of DNA breakage was explored. The method was able to detect 5 fg/μL camel DNA and highly processed food containing 0.01% camel meat with a high confidence level.
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9

MENDOZA-ROMERO, LUIS, EDWARD L. C. VERKAAR, PAUL H. SAVELKOUL, et al. "Real-Time PCR Detection of Ruminant DNA." Journal of Food Protection 67, no. 3 (2004): 550–54. http://dx.doi.org/10.4315/0362-028x-67.3.550.

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To control the spread of bovine spongiform encephalopathy, several DNA methods have been described for the detection of the species origin of meat and bone meal. Most of these methods are based on the amplification of a mitochondrial DNA segment. We have developed a semiquantitative method based on real-time PCR for detection of ruminant DNA, targeting an 88-bp segment of the ruminant short interspersed nuclear element Bov-A2. This method is specific for ruminants and is able to detect as little as 10 fg of bovine DNA. Autoclaving decreased the amount of detectable DNA, but positive signals were observed in feeding stuff containing 10% bovine material if this had not been rendered in accordance with the regulations, i.e., heated at 134°C for 3 instead of 20 min.
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10

HU, LIANXIA, SHUFEI ZHANG, YULING XUE, et al. "Rapid Identification of Pseudomonas fluorescens Harboring Thermostable Alkaline Protease by Real-Time Loop-Mediated Isothermal Amplification." Journal of Food Protection 85, no. 3 (2021): 414–23. http://dx.doi.org/10.4315/jfp-21-272.

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ABSTRACT Thermostable alkaline protease (TAP) harbored by Pseudomonas fluorescens decomposes protein in milk and dairy products, leading to milk and dairy product spoilage during storage. Thus, a specific, sensitive, rapid, and simple method is required to detect TAP-harboring P. fluorescens. Two sets of primers targeting the aprX and gyrB genes of P. fluorescens were designed. The detection system and conditions were optimized, and a real-time loop-mediated isothermal amplification (real-time LAMP) method was developed for the simultaneous detection of TAP-harboring P. fluorescens in two separate reaction tubes. The phylogenetic tree targeting aprX showed that P. fluorescens and Pseudomonas lurida clustered on the same branch. The phylogenetic tree targeting gyrB showed that P. fluorescens clustered on the same branch with 95% confidence value, whereas P. lurida clustered on different branches. DNA of 16 strains of P. fluorescens and 34 strains of non–P. fluorescens was detected by real-time LAMP. TAP-harboring P. fluorescens can only be identified when the real-time LAMP detection results of both aprX and gyrB are positive. The dissociation temperatures of aprX and gyrB in the real-time LAMP–amplified products were approximately 90.0 and 88.0°C, respectively. The detection limits of the real-time LAMP targeting aprX and gyrB were 4.9 CFU per reaction in pure culture and 2.2 CFU per reaction in skimmed milk. The coefficient of variation of the repeatability test was less than 2%, indicating that the established real-time LAMP of P. fluorescens targeting gyrB and aprX has good stability and repeatability. Real-time LAMP was used to test 200 raw milk samples for the presence of TAP-harboring P. fluorescens in 3 h, and the coincidence rate of the results with those obtained using the traditional method, which takes at least 5 to 7 days, was 100%. Real-time LAMP will be a practical and effective method for accurate and rapid identification of TAP-harboring P. fluorescens in raw milk. HIGHLIGHTS
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11

Kridel, Donald, Dan Dolk, and David Castillo. "Adaptive Modeling and Dynamic Targeting for Real Time Analytics in Mobile Advertising." International Journal of Systems and Service-Oriented Engineering 7, no. 2 (2017): 24–39. http://dx.doi.org/10.4018/ijssoe.2017040102.

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Mobile marketing campaigns are now largely deployed through demand side platforms (DSPs) who provide dynamic customer targeting and a performance-intensive real-time bidding (RTB) version of predictive analytics as a service. Matching users with the campaigns they are most likely to engage with in extreme real-time environments requires adaptive model management, advanced parallel processing hardware/software, and the integration of multiple very large databases. The authors present (1) an adaptive modeling strategy to satisfy the performance thresholds of 40 to 100ms for DSPs to decide whether and how much to bid for a potential client to receive a particular advertisement via their mobile device. (2) a dynamic customer profiling technique to map mobile devices to specific lattices (geographic locations), and to track user behavior via device-histories. In this “big data” decision environment, analytic model management is automated via model feedback loops which adjust the models dynamically as real-time data streams in.
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12

Antwerpen, Markus H., Pia Zimmermann, Kevin Bewley, Dimitrios Frangoulidis, and Hermann Meyer. "Real-time PCR system targeting a chromosomal marker specific for Bacillus anthracis." Molecular and Cellular Probes 22, no. 5-6 (2008): 313–15. http://dx.doi.org/10.1016/j.mcp.2008.06.001.

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13

Ngounga, Tatsiana, Isabelle Pagnier, Dorine-Gaelle Ikanga Reteno, Didier Raoult, Bernard La Scola, and Philippe Colson. "Real-Time PCR Systems Targeting Giant Viruses of Amoebae and Their Virophages." Intervirology 56, no. 6 (2013): 413–23. http://dx.doi.org/10.1159/000354563.

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14

Ferdin, Jana, Tjaša Cerar, Franc Strle, and Eva Ružić-Sabljić. "Evaluation of real-time PCR targeting hbb gene for Borrelia species identification." Journal of Microbiological Methods 82, no. 2 (2010): 115–19. http://dx.doi.org/10.1016/j.mimet.2010.04.009.

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15

Bai, Jie, Julie Tzu-Wen Wang, Kuo-Ching Mei, Wafa T. Al-Jamal, and Khuloud T. Al-Jamal. "Real-time monitoring of magnetic drug targeting using fibered confocal fluorescence microscopy." Journal of Controlled Release 244 (December 2016): 240–46. http://dx.doi.org/10.1016/j.jconrel.2016.07.026.

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16

Ryan, Julie L., Hongxin Fan, Sally L. Glaser, Steven A. Schichman, Nancy Raab-Traub, and Margaret L. Gulley. "Epstein-Barr Virus Quantitation by Real-Time PCR Targeting Multiple Gene Segments." Journal of Molecular Diagnostics 6, no. 4 (2004): 378–85. http://dx.doi.org/10.1016/s1525-1578(10)60535-1.

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17

Niznikiewicz, Margaret, Kana Okano, Clemens Bauer, et al. "O4.4. REAL TIME FMRI NEUROFEEDBACK TARGETING STG AND DMN REDUCES AUDITORY HALLUCINATIONS." Schizophrenia Bulletin 46, Supplement_1 (2020): S10. http://dx.doi.org/10.1093/schbul/sbaa028.021.

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Abstract Background Auditory hallucinations (AH) are one of the core symptoms of schizophrenia (SZ) and constitute a significant source of suffering and disability. One third of SZ patients experience pharmacology-resistant AH, so an alternative/complementary treatment strategy is needed to alleviate this debilitating condition. In this study, real-time functional Magnetic Resonance Imaging neurofeedback (rt-fMRI NFB), a non-invasive technique, was used to help 10 SZ patients modulate their brain activity in key brain regions belonging to the network involved in the experience of auditory hallucinations. In two experiments we selected two different brain targets. 1. the superior temporal gyrus (STG) and 2. default mode network (DMN)-central executive network (CEN) connectivity. STG is a key area in the neurophysiology of AH. Hyperactivation of the default mode network (DMN) and of the superior temporal gyrus (STG) in SZ has been shown in imaging studies. Furthermore, several studies point to reduced anticorrelation between the DMN and the central executive network (CEN). Finally, DMN hyperconnectivity has been associated with positive symptoms such as AHs while reduced DMN anticorrelations have been associated with cognitive impairment. Methods In the STG-focused NFB experiment, subjects were trained to upregulate the STG activity while listening to their own voice recording and downregulate it while ignoring a stranger’s voice recording in the course of 21 min NFB session. Visual feedback was provided to subjects at the end of each run from their own STG activity in the form of a thermometer. AH were assessed with auditory hallucination scale pre-NFB and within a week after the NFB session. The DMN-CEN focused NFB experiment was conducted about 1 month later to minimize the carry over effects from the STG-focused NFB and was designed to help SZ patients modulate their DMN and CEN networks. DMN and CEN networks were defined individually for each subject. The goal of the task was to increase CEN-DMN anti-correlations. To achieve that patients were provided with meditation strategies to guide their performance. Feedback was provided in the form of a ball that traveled up if the modulation of DMN-CEN connectivity was successful and traveled down if it was not successful. AH measures were taken before the NFB session and within a week after the session. Results In the STG-focused NFB task, significant STG activation reduction was found in the comparison of pre- relative to post-NFB in the condition of ignoring another person’s voice (p<0.05), FWE-TFCE corrected. AH were also significantly reduced (p<0.01). Importantly, significant correlation was found between reductions in the STG activation and AH reductions (r=.83). In the DMN-CEN focused NFB task, significant increase in the anti-correlations between medial prefrontal cortex (mPFC) and dorsolateral prefrontal cortex (DLPFC) (p<0.05) was observed as well as significant reduction in the mPFC-PCC connectivity (p <0.05), in the pre-post NFB comparisons. AH were significantly reduced in post- relative to pre-NFB comparison (p<0.02). Finally, there was a significant correlation between individual scores in mPFC-STG connectivity and AH reductions. Discussion These the two experiments suggest that targeting both the STG BOLD activation and DMN-CEN connectivity in NFB tasks aimed at AH reduction result both in brain changes and in AH reductions. Together, these results provide strong preliminary support for the NFB use as a means to impact brain function leading to reductions in AH in SZ. Importantly, these results suggest that AH result from brain abnormalities in a network of brain regions and that targeting a brain region belonging to this network will lead to AH symptom reduction.
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18

Chase, Catherine J., Melanie P. Ulrich, Leonard P. Wasieloski, et al. "Real-Time PCR Assays Targeting a Unique Chromosomal Sequence of Yersinia pestis." Clinical Chemistry 51, no. 10 (2005): 1778–85. http://dx.doi.org/10.1373/clinchem.2005.051839.

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Abstract Background: Yersinia pestis, the causative agent of the zoonotic infection plague, is a major concern as a potential bioweapon. Current real-time PCR assays used for Y. pestis detection are based on plasmid targets, some of which may generate false-positive results. Methods: Using the yp48 gene of Y. pestis, we designed and tested 2 real-time TaqMan® minor groove binder (MGB) assays that allowed us to use chromosomal genes as both confirmatory and differential targets for Y. pestis. We also designed several additional assays using both Simple-Probe® and MGB Eclipse™ probe technologies for the selective differentiation of Yersinia pseudotuberculosis from Y. pestis. These assays were designed around a 25-bp insertion site recently identified within the yp48 gene of Y. pseudotuberculosis. Results: The Y. pestis-specific assay distinguished this bacterium from other Yersinia species but had unacceptable low-level detection of Y. pseudotuberculosis, a closely related species. Simple-Probe and MGB Eclipse probes specific for the 25-bp insertion detected only Y. pseudotuberculosis DNA. Probes that spanned the deletion site detected both Y. pestis and Y. pseudotuberculosis DNA, and the 2 species were clearly differentiated by a post-PCR melting temperature (Tm) analysis. The Simple-Probe assay produced an almost 7 °C Tm difference and the MGB Eclipse probe a slightly more than 4 °C difference. Conclusions: Our method clearly discriminates Y. pestis DNA from all other Yersinia species tested and from the closely related Y. pseudotuberculosis. These chromosomal assays are important both to verify the presence of Y. pestis based on a chromosomal target and to easily distinguish it from Y. pseudotuberculosis.
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Aydogan, Dogu Baran, Victor Hugo Souza, Pantelis Lioumis, and Risto J. Ilmoniemi. "Targeting networks of the brain with real-time tractography-based TMS neuronavigation." Brain Stimulation 16, no. 1 (2023): 163–64. http://dx.doi.org/10.1016/j.brs.2023.01.148.

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20

Wang, Xiao-Qiang, Mengyun Peng, Chu-Xin Li, et al. "Real-Time Imaging of Free Radicals for Mitochondria-Targeting Hypoxic Tumor Therapy." Nano Letters 18, no. 11 (2018): 6804–11. http://dx.doi.org/10.1021/acs.nanolett.8b02670.

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21

Guilbaud, Morgan, Pierre de Coppet, Fabrice Bourion, Cinta Rachman, Hervé Prévost, and Xavier Dousset. "Quantitative Detection of Listeria monocytogenes in Biofilms by Real-Time PCR." Applied and Environmental Microbiology 71, no. 4 (2005): 2190–94. http://dx.doi.org/10.1128/aem.71.4.2190-2194.2005.

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ABSTRACT A quantitative method based on a real-time PCR assay to enumerate Listeria monocytogenes in biofilms was developed. The specificity for L. monocytogenes of primers targeting the listeriolysin gene was demonstrated using a SYBR Green I real-time PCR assay. The number of L. monocytogenes detected growing in biofilms was 6 × 102 CFU/cm2.
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22

Radomski, Nicolas, Françoise S. Lucas, Régis Moilleron, Emmanuelle Cambau, Sophie Haenn, and Laurent Moulin. "Development of a Real-Time qPCR Method for Detection and Enumeration of Mycobacterium spp. in Surface Water." Applied and Environmental Microbiology 76, no. 21 (2010): 7348–51. http://dx.doi.org/10.1128/aem.00942-10.

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ABSTRACT A real-time quantitative PCR method was developed for the detection and enumeration of Mycobacterium spp. from environmental samples and was compared to two other methods already described. The results showed that our method, targeting 16S rRNA, was more specific than the two previously published real-time quantitative PCR methods targeting another 16S rRNA locus and the hsp65 gene (100% versus 44% and 91%, respectively).
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Nitsche, Andreas, Mathias Büttner, Sonja Wilhelm, Georg Pauli, and Hermann Meyer. "Real-Time PCR Detection of Parapoxvirus DNA,." Clinical Chemistry 52, no. 2 (2006): 316–19. http://dx.doi.org/10.1373/clinchem.2005.060335.

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Abstract Background: Detection of parapoxviruses is important in various animals as well as in humans as zoonotic infections. Reliable detection of parapoxviruses is fundamental for the exclusion of other rash-causing illnesses, for both veterinarians and medical practitioners. To date, however, no real-time PCR assay for the detection of parapoxviruses has been reported. Methods: A minor groove binder–based quantitative real-time PCR assay targeting the B2L gene of parapoxviruses was developed on the ABI Prism and the LightCycler platforms. Results: The real-time PCR assay successfully amplified DNA fragments from a total of 41 parapoxvirus strains and isolates representing the species orf virus, bovine papular stomatitis virus, pseudocowpoxvirus, and sealpoxvirus. Probit analysis gave a limit of detection of 4.7 copies per assay (95% confidence interval, 3.7–6.8 copies per reaction). Scabs contain a sufficient amount of parapoxvirus DNA and can therefore be used for PCR without any DNA preparation step. No cross-reactivity to human, bovine, or sheep genomic DNA or other DNA viruses, including orthopoxviruses, molluscum contagiosum viruses, and yaba-like disease viruses, was observed. Conclusion: The presented assay is suitable for the detection of parapoxvirus infections in clinical material of human and animal origin.
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Liu, Bei, Lirong Sun, Xijian Lu, et al. "Real-time drug release monitoring from pH-responsive CuS-encapsulated metal–organic frameworks." RSC Advances 12, no. 18 (2022): 11119–27. http://dx.doi.org/10.1039/d1ra09320g.

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A metal–organic framework-based nanotheranostic system was fabricated to achieve the capabilities of tumor-targeting, real-time monitoring of pH-responsive drug release and combined chemo-photothermal therapy.
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Clardy, Susan M., Rainer H. Kohler, Claudio Vinegoni, Yoshiko Iwamoto, Edmund J. Keliher, and Ralph Weissleder. "Two-photon imaging of pancreatic beta cells in real time in vivo." TECHNOLOGY 04, no. 02 (2016): 130–34. http://dx.doi.org/10.1142/s2339547816200028.

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Here we present the first generation of two-photon beta cell specific in vivo imaging probes based on GLP1R targeting peptides. Among the three compounds of potential interest, we found quite unexpectedly that a squarine-rotaxane conjugate (2PEx-647) had near ideal in vivo imaging characteristics.
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Huang, Xiaoqian, Mohamad Halwani, Rajkumar Muthusamy, et al. "Real-time grasping strategies using event camera." Journal of Intelligent Manufacturing 33, no. 2 (2022): 593–615. http://dx.doi.org/10.1007/s10845-021-01887-9.

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AbstractRobotic vision plays a key role for perceiving the environment in grasping applications. However, the conventional framed-based robotic vision, suffering from motion blur and low sampling rate, may not meet the automation needs of evolving industrial requirements. This paper, for the first time, proposes an event-based robotic grasping framework for multiple known and unknown objects in a cluttered scene. With advantages of microsecond-level sampling rate and no motion blur of event camera, the model-based and model-free approaches are developed for known and unknown objects’ grasping respectively. The event-based multi-view approach is used to localize the objects in the scene in the model-based approach, and then point cloud processing is utilized to cluster and register the objects. The proposed model-free approach, on the other hand, utilizes the developed event-based object segmentation, visual servoing and grasp planning to localize, align to, and grasp the targeting object. Using a UR10 robot with an eye-in-hand neuromorphic camera and a Barrett hand gripper, the proposed approaches are experimentally validated with objects of different sizes. Furthermore, it demonstrates robustness and a significant advantage over grasping with a traditional frame-based camera in low-light conditions.
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Gubala, Aneta J., and David F. Proll. "Molecular-Beacon Multiplex Real-Time PCR Assay for Detection of Vibrio cholerae." Applied and Environmental Microbiology 72, no. 9 (2006): 6424–28. http://dx.doi.org/10.1128/aem.02597-05.

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ABSTRACT A multiplex real-time PCR assay was developed using molecular beacons for the detection of Vibrio cholerae by targeting four important virulence and regulatory genes. The specificity and sensitivity of this assay, when tested with pure culture and spiked environmental water samples, were high, surpassing those of currently published PCR assays for the detection of this organism.
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Tumurbaatar, Batgerel, Chan-Hee Park, Jun Hee Lee, and Cheol Sang Kim. "Controls of Trajectories for Targeting of Magnetic Robotics in body." Embedded Selforganising Systems 4, no. 1 (2017): 2–5. http://dx.doi.org/10.14464/ess41197.

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This paper presents a novel method to actively control magnetic field in a region-of-interest using three pairs electromagnetic coils system referred to here as extended distributed treatment Robotics. The developed controls of trajectories for targeting of magnetic robotics in body system contains hardware, software and magnetic Robotics/nanoscale material and the in vitro manipulation in real time. In this study, we used six identical solenoids coil placed on an XYZ-axis and the electromagnet was powered by current that can generate a high-gradient magnetic field in the desired direction. Real-time video microscopy supported by the LabVIEW vision system is integrated into the developed system for real-time monitoring. Moreover, the detection of object function is done through NI Vision Assistant, tracking function is through Math Script node in the LabVIEW simulation and ROI magnetic field actual measurement is done by the real-time magnetic sensor. The motion speed and direction of the Magnetic Robotics can also be manipulated using EMM system and Joystick controller.
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Paulraj, Balachandar. "Enhancing Data Engineering Frameworks for Scalable Real-Time Marketing Solutions." Integrated Journal for Research in Arts and Humanities 3, no. 5 (2023): 309–15. http://dx.doi.org/10.55544/ijrah.3.5.34.

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This report discusses improvements to the data engineering frameworks for cost-efficient real-time marketing environment, with the applications in mobile advertising and smart city analytics. The proposed framework combines the processing of time-series data with map-reduce (M-R) systems in order to compensate for inefficiencies in the execution of temporal queries and the processing of real-time data streams. It enables effective behavioral targeting in mobile marketing and helps integrate various domains’ real-time data for smart city utilization. Specific examples that illustrate further refinements in the algorithms are showcased through case study and experimental evidence where gains in throughput, data reliability, and system effectiveness are quantified in real-time Marketing and Smart City applications.
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Edvinsson, B., M. Lappalainen, and B. Evengård. "Real-time PCR targeting a 529-bp repeat element for diagnosis of toxoplasmosis." Clinical Microbiology and Infection 12, no. 2 (2006): 131–36. http://dx.doi.org/10.1111/j.1469-0691.2005.01332.x.

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31

Whitfield-Gabrieli, Susan, Clemens Bauer, Kana Okano, et al. "M64. Real Time fMRI Feedback Targeting Default Mode Network (DMN) Reduces Auditory Hallucinations." Schizophrenia Bulletin 43, suppl_1 (2017): S233. http://dx.doi.org/10.1093/schbul/sbx022.059.

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32

Robesova, Blanka, Monika Bajerova, Kvetoslava Liskova, et al. "TaqMan based real time PCR assay targeting EML4-ALK fusion transcripts in NSCLC." Lung Cancer 85, no. 1 (2014): 25–30. http://dx.doi.org/10.1016/j.lungcan.2014.04.002.

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33

Weinhous, Martin S., Scott Ameduri, Wyatt Newman, John Greskovich, and Roger M. Macklis. "Feasibility of real-time image-directed beam-targeting in moving-target radiation therapy." International Journal of Radiation Oncology*Biology*Physics 42, no. 1 (1998): 368. http://dx.doi.org/10.1016/s0360-3016(98)80589-0.

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Torshabi, A. E., Andrea Pella, Marco Riboldi, and Guido Baroni. "Targeting Accuracy in Real-time Tumor Tracking via External Surrogates: A Comparative Study." Technology in Cancer Research & Treatment 9, no. 6 (2010): 551–61. http://dx.doi.org/10.1177/153303461000900603.

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Zhou, Jun, Yi-Ting Tsai, Hong Weng, David W. Baker, and Liping Tang. "Real time monitoring of biomaterial-mediated inflammatory responses via macrophage-targeting NIR nanoprobes." Biomaterials 32, no. 35 (2011): 9383–90. http://dx.doi.org/10.1016/j.biomaterials.2011.08.064.

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36

Giaz, Agnese, Marco Galoppo, Nikolay Ampilogov, et al. "ORIGIN, an EU project targeting real-time 3D dose imaging and source localization." Nuclear Inst. and Methods in Physics Research, A 1048, March 2023 (2023): 167999. https://doi.org/10.1016/j.nima.2022.167999.

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The ORIGIN project targets the production and qualification of a real-time radiation dose imaging and source localization system for both Low Dose Rate (LDR) and High Dose Rate (HDR) brachytherapy treatments, namely radiotherapy based on the use of radioactive sources implanted in the patient’s body. This goal will be achieved through a 16-fiber sensor system, engineered to house in a clear-fiber tip a small volume of the scintillator to allow point-like measurements of the delivered dose. Each fiber is optically coupled to a sensor with single photon sensitivity (Silicon Photomultipliers — SiPMs) operating in counting mode. The readout is based on the CITIROC1A ASIC by WEEROC, embedded in the FERS-DT5202 scalable platform designed by CAEN S.p.A. Linearity and sensitivity together with the fiber response uniformity, system stability, and measurement reproducibility are key features for a instrument aiming to perform dose measurements. Characterization was carried out in the laboratory, using an X-ray cabinet; preliminary dose rate measurements were performed in clinical conditions.
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Toreti, Andrea, Marian Bratu, Evelyn Müller, et al. "Advancing Near-Real-Time Quality Controls of Meteorological Observations." Bulletin of the American Meteorological Society 103, no. 4 (2022): E1078—E1087. http://dx.doi.org/10.1175/bams-d-21-0171.1.

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Abstract Meteorological observations from ground weather stations are of the upmost importance to implement and run sectoral climate services and to perform scientific activities, such as models’ evaluation and data assimilation. However, meteorological observations may be affected by errors deteriorating the quality and the reliability of the products based on these data. To avoid such a cascade of effects, it is essential to quality check meteorological observations in near–real time. A new system based on several levels of increasing complexity checks is proposed here. Besides the standard quality controls, this system applies dynamic and adaptive checks targeting exactly the locations and the data under analysis. The proposed system is designed to be flexible and easily modifiable thanks to its combined R and XML implementation. To facilitate its applicability, the proposed system is made available as a free open-source software: QuackMe.
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Yano, Shuya, Hiroshi Tazawa, Hiroyuki Kishimoto, Shunsuke Kagawa, Toshiyoshi Fujiwara, and Robert M. Hoffman. "Real-Time Fluorescence Image-Guided Oncolytic Virotherapy for Precise Cancer Treatment." International Journal of Molecular Sciences 22, no. 2 (2021): 879. http://dx.doi.org/10.3390/ijms22020879.

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Oncolytic virotherapy is one of the most promising, emerging cancer therapeutics. We generated three types of telomerase-specific replication-competent oncolytic adenovirus: OBP-301; a green fluorescent protein (GFP)-expressing adenovirus, OBP-401; and Killer-Red-armed OBP-301. These oncolytic adenoviruses are driven by the human telomerase reverse transcriptase (hTERT) promoter; therefore, they conditionally replicate preferentially in cancer cells. Fluorescence imaging enables visualization of invasion and metastasis in vivo at the subcellular level; including molecular dynamics of cancer cells, resulting in greater precision therapy. In the present review, we focused on fluorescence imaging applications to develop precision targeting for oncolytic virotherapy. Cell-cycle imaging with the fluorescence ubiquitination cell cycle indicator (FUCCI) demonstrated that combination therapy of an oncolytic adenovirus and a cytotoxic agent could precisely target quiescent, chemoresistant cancer stem cells (CSCs) based on decoying the cancer cells to cycle to S-phase by viral treatment, thereby rendering them chemosensitive. Non-invasive fluorescence imaging demonstrated that complete tumor resection with a precise margin, preservation of function, and prevention of distant metastasis, was achieved with fluorescence-guided surgery (FGS) with a GFP-reporter adenovirus. A combination of fluorescence imaging and laser ablation using a KillerRed-protein reporter adenovirus resulted in effective photodynamic cancer therapy (PDT). Thus, imaging technology and the designer oncolytic adenoviruses may have clinical potential for precise cancer targeting by indicating the optimal time for administering therapeutic agents; accurate surgical guidance for complete resection of tumors; and precise targeted cancer-specific photosensitization.
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Yu, Y., C. Lee, and S. Hwang. "Analysis of community structures in anaerobic processes using a quantitative real-time PCR method." Water Science and Technology 52, no. 1-2 (2005): 85–91. http://dx.doi.org/10.2166/wst.2005.0502.

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The methanogenic community structures of four different anaerobic processes were characterized using a quantitative real-time PCR with group-specific primer and probe sets targeting the 16S rRNA gene (rDNA). The group specific primer and probe sets were developed and used to detect the orders Methanosarcinales, and the families Methanosarcinaceae and Methanosaetaceae. Two separate sets targeting the domains Archaea and Bacteria were also used. Each microbial population in different anaerobic processes was determined and the relative abundance in the system was compared with each other. Dominant methanogenic populations and the community structures in the processes were varied by hydraulic retention time and acetate concentration. This indicates that the real-time PCR method with the primer and probe sets is a promising tool to analyze community structures in anaerobic processes.
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40

He, Boning, Guoli Zhu, Lei Han, and Dailin Zhang. "Adaptive-Neuro-Fuzzy-Based Information Fusion for the Attitude Prediction of TBMs." Sensors 21, no. 1 (2020): 61. http://dx.doi.org/10.3390/s21010061.

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In a tunneling boring machine (TBM), to obtain the attitude in real time is very important for a driver. However, the current laser targeting system has a large delay before obtaining the attitude. So, an adaptive-neuro-fuzzy-based information fusion method is proposed to predict the attitude of a laser targeting system in real time. In the proposed method, a dual-rate information fusion is used to fuse the information of a laser targeting system and a two-axis inclinometer, and then obtain roll and pitch angles with a higher rate and provide a smoother attitude prediction. Considering that a measurement error exists, the adaptive neuro-fuzzy inference system (ANFIS) is proposed to model the measurement error, and then the ANFIS-based model is combined with the dual-rate information fusion to achieve high performance. Experimental results show the ANFIS-based information fusion can provide higher real-time performance and accuracy of the attitude prediction. Experimental results also verify that the ANFIS-based information fusion can solve the problem of the laser targeting system losing signals.
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Hünniger, Tim, Christine Felbinger, Hauke Wessels, et al. "Food Targeting: A Real-Time PCR Assay Targeting 16S rDNA for Direct Quantification ofAlicyclobacillusspp. Spores after Aptamer-Based Enrichment." Journal of Agricultural and Food Chemistry 63, no. 17 (2015): 4291–96. http://dx.doi.org/10.1021/acs.jafc.5b00874.

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Vincart, Benoit, Ricardo De Mendonça, Sylvianne Rottiers, Françoise Vermeulen, Marc J. Struelens, and Olivier Denis. "A specific real-time PCR assay for the detection of Bordetella pertussis." Journal of Medical Microbiology 56, no. 7 (2007): 918–20. http://dx.doi.org/10.1099/jmm.0.46947-0.

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A novel real-time PCR (RT-PCR) assay was developed for detection of Bordetella pertussis in respiratory specimens by targeting the pertactin gene. In vitro evaluation with reference strains and quality control samples showed analytical sensitivity equivalent to and specificity superior to those of PCR assays which target the IS481 element. The pertactin-based RT-PCR assay offers better discrimination between B. pertussis and other Bordetella species than previously described assays.
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Wang, Deguo, Yongzhen Wang, Kai Zhu, et al. "Detection of Cassava Component in Sweet Potato Noodles by Real-Time Loop-mediated Isothermal Amplification (Real-time LAMP) Method." Molecules 24, no. 11 (2019): 2043. http://dx.doi.org/10.3390/molecules24112043.

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Sweet potato (Ipomoea batatas) noodles are a traditional Chinese food with a high nutritional value; however, starch adulteration is a big concern. The objective of this study was to develop a reliable method for the rapid detection of cassava (Manihot esculenta) components in sweet potato noodles to protect consumers from commercial adulteration. Five specific Loop-mediated Isothermal Amplification (LAMP) primers targeting the internal transcribed spacer (ITS) of cassava were designed, genomic DNA was extracted, the LAMP reaction system was optimized, and the specificity of the primers was verified with genomic DNA of cassava, Ipomoea batatas, Zea mays, and Solanum tuberosum; the detection limit was determined with a serial dilution of adulterated sweet potato starch with cassava starch, and the real-time LAMP method for the detection of the cassava-derived ingredient in sweet potato noodles was established. The results showed that the real-time LAMP method can accurately and specifically detect the cassava component in sweet potato noodles with a detection limit of 1%. Furthermore, the LAMP assay was validated using commercial sweet potato noodle samples, and results showed that 57.7% of sweet potato noodle products (30/52) from retail markets were adulterated with cassava starch in China. This study provides a promising solution for facilitating the surveillance of the commercial adulteration of sweet potato noodles from retail markets.
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Lacroix, Lise-Marie, Fabien Delpech, Céline Nayral, Sébastien Lachaize, and Bruno Chaudret. "New generation of magnetic and luminescent nanoparticles for in vivo real-time imaging." Interface Focus 3, no. 3 (2013): 20120103. http://dx.doi.org/10.1098/rsfs.2012.0103.

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A new generation of optimized contrast agents is emerging, based on metallic nanoparticles (NPs) and semiconductor nanocrystals for, respectively, magnetic resonance imaging (MRI) and near-infrared (NIR) fluorescent imaging techniques. Compared with established contrast agents, such as iron oxide NPs or organic dyes, these NPs benefit from several advantages: their magnetic and optical properties can be tuned through size, shape and composition engineering, their efficiency can exceed by several orders of magnitude that of contrast agents clinically used, their surface can be modified to incorporate specific targeting agents and antifolding polymers to increase blood circulation time and tumour recognition, and they can possibly be integrated in complex architecture to yield multi-modal imaging agents. In this review, we will report the materials of choice based on the understanding of the basic physics of NIR and MRI techniques and their corresponding syntheses as NPs. Surface engineering, water transfer and specific targeting will be highlighted prior to their first use for in vivo real-time imaging. Highly efficient NPs that are safer and target specific are likely to enter clinical application in a near future.
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Mohyeldin, Ahmed, Russell R. Lonser, and J. Bradley Elder. "Real-time magnetic resonance imaging-guided frameless stereotactic brain biopsy: technical note." Journal of Neurosurgery 124, no. 4 (2016): 1039–46. http://dx.doi.org/10.3171/2015.5.jns1589.

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OBJECT The object of this study was to assess the feasibility, accuracy, and safety of real-time MRI-compatible frameless stereotactic brain biopsy. METHODS Clinical, imaging, and histological data in consecutive patients who underwent stereotactic brain biopsy using a frameless real-time MRI system were analyzed. RESULTS Five consecutive patients (4 males, 1 female) were included in this study. The mean age at biopsy was 45.8 years (range 29–60 years). Real-time MRI permitted concurrent display of the biopsy cannula trajectory and tip during placement at the target. The mean target depth of biopsied lesions was 71.3 mm (range 60.4–80.4 mm). Targeting accuracy analysis revealed a mean radial error of 1.3 ± 1.1 mm (mean ± standard deviation), mean depth error of 0.7 ± 0.3 mm, and a mean absolute tip error of 1.5 ± 1.1 mm. There was no correlation between target depth and absolute tip error (Pearson product-moment correlation coefficient, r = 0.22). All biopsy cannulae were placed at the target with a single penetration and resulted in a diagnostic specimen in all cases. Histopathological evaluation of biopsy samples revealed dysembryoplastic neuroepithelial tumor (1 case), breast carcinoma (1 case), and glioblastoma multiforme (3 cases). CONCLUSIONS The ability to place a biopsy cannula under real-time imaging guidance permits on-the-fly alterations in the cannula trajectory and/or tip placement. Real-time imaging during MRI-guided brain biopsy provides precise safe targeting of brain lesions.
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Hasan, Mohammad Rubayet, Rusung Tan, Ghada N. Al-Rawahi, Eva Thomas, and Peter Tilley. "Evaluation of Amplification Targets for the Specific Detection ofBordetella pertussisUsing Real-Time Polymerase Chain Reaction." Canadian Journal of Infectious Diseases and Medical Microbiology 25, no. 4 (2014): 217–21. http://dx.doi.org/10.1155/2014/763128.

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BACKGROUND:Bordetella pertussisinfections continue to be a major public health challenge in Canada. Polymerase chain reaction (PCR) assays to detectB pertussisare typically based on the multicopy insertion sequence IS481, which offers high sensitivity but lacks species specificity.METHODS: A novelB pertussisreal-time PCR assay based on the porin gene was tested in parallel with several previously published assays that target genes such as IS481,ptx-promoter, pertactin and a putative thialase. The assays were evaluated using a reference panel of common respiratory bacteria including differentBordetellaspecies and 107 clinical nasopharyngeal specimens. Discrepant results were confirmed by sequencing the PCR products.RESULTS: Analytical sensitivity was highest for the assay targeting the IS481element; however, the assay lacked specificity forB pertussisin the reference panel and in the clinical samples. False-positive results were also observed with assays targeting theptx-promoter and pertactin genes. A PCR assay based on the thialase gene was highly specific but failed to detect all reference strains ofB pertussis. However, a novel assay targeting the porin gene demonstrated high specificity forB pertussisboth in the reference panel and in clinical samples and, based on sequence-confirmed results, correctly predicted allB pertussis-positive cases in clinical samples. According to Probit regression analysis, the 95% detection limit of the new assay was 4 colony forming units/reaction.CONCLUSION: A novel porin assay forB pertussisdemonstrated superior performance and may be useful for improved molecular detection ofB pertussisin clinical specimens.
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Minero, Gabriel Antonio S., Catarina Nogueira, Giovanni Rizzi, et al. "Sequence-specific validation of LAMP amplicons in real-time optomagnetic detection of Dengue serotype 2 synthetic DNA." Analyst 142, no. 18 (2017): 3441–50. http://dx.doi.org/10.1039/c7an01023k.

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Blanda, Valeria, Rosalia D’Agostino, Elisabetta Giudice, et al. "New Real-Time PCRs to Differentiate Rickettsia spp. and Rickettsia conorii." Molecules 25, no. 19 (2020): 4431. http://dx.doi.org/10.3390/molecules25194431.

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Rickettsia species are an important cause of emerging infectious diseases in people and animals, and rickettsiosis is one of the oldest known vector-borne diseases. Laboratory diagnosis of Rickettsia is complex and time-consuming. This study was aimed at developing two quantitative real-time PCRs targeting ompB and ompA genes for the detection, respectively, of Rickettsia spp. and R. conorii DNA. Primers were designed following an analysis of Rickettsia gene sequences. The assays were optimized using SYBR Green and TaqMan methods and tested for sensitivity and specificity. This study allowed the development of powerful diagnostic methods, able to detect and quantify Rickettsia spp. DNA and differentiate R. conorii species.
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Peng, Qingyun, Xin Zhong, Wei Lei, Guren Zhang, and Xin Liu. "Detection ofOphiocordyceps sinensisin soil by quantitative real-time PCR." Canadian Journal of Microbiology 59, no. 3 (2013): 204–9. http://dx.doi.org/10.1139/cjm-2012-0490.

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Ophiocordyceps sinensis, one of the best known entomopathogenic fungi in traditional Chinese medicine, parasitizes larvae of the moth genus Thitarodes, which lives in soil tunnels. However, little is known about the spatial distribution of O. sinensis in the soil. We established a protocol for DNA extraction, purification, and quantification of O. sinensis in soil with quantitative real-time PCR targeting the internal transcribed spacer region. The method was assessed using 34 soil samples from Tibet. No inhibitory effects in purified soil DNA extracts were detected. The standard curve method for absolute DNA quantification generated crossing point values that were strongly and linearly correlated to the log10of the initial amount of O. sinensis genomic DNA (r2= 0.999) over 7 orders of magnitude (4 × 101to 4 × 107fg). The amplification efficiency and y-intercept value of the standard curve were 1.953 and 37.70, respectively. The amount of O. sinensis genomic DNA decreased with increasing soil depth and horizontal distance from a sclerotium (P < 0.05). Our protocol is rapid, specific, sensitive, and provides a powerful tool for quantification of O. sinensis from soil.
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Afonso, Vladimir, Henrique Maich, Luan Audibert, et al. "Hardware Implementation for the HEVC Fractional Motion Estimation Targeting Real-Time and Low-Energy." Journal of Integrated Circuits and Systems 11, no. 2 (2016): 106–20. http://dx.doi.org/10.29292/jics.v11i2.435.

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This paper presents an energy-aware and high-throughput hardware design for the Fractional Motion Estimation (FME) compliant with the High Efficiency Video Coding (HEVC) standard. An extensive software evaluation was performed to guide the hardware design. The adopted strategy mainly consists in using only the four squareshaped Prediction Unit (PU) sizes rather than using all 24 possible PU sizes in the Motion Estimation (ME). This approach reduces about 59% the total encoding time and, as a penalty, it leads to an increase of only 4% in the bit rate for the same image quality. Together with this simplification, a multiplierless approach, algebraic optimizations and low-power techniques were applied to the hardware design to reduce the hardware-resource usage and the energy consumption, maintaining a high processing rate. The architecture was described in VHDL and the synthesis results for ASIC 45nm Nangate standard cells demonstrate that the developed architecture is able to process Ultra-High Definition (UHD) 2160p videos at 60 frames per second (fps), with the lowest power consumption and the lowest hardware-resource usage among the related works.
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