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Uy, Melanie Rose Y. "RECAP: Resilience Enhancement that Combats Alcohol Problems." Xavier University / OhioLINK, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=xavier1493395921847418.
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Danzl, Megan M. "Developing the Rehabilitation Education for Caregivers and Patients (RECAP) Model: Application to Physical Therapy in Stroke Rehabilitation." UKnowledge, 2013. http://uknowledge.uky.edu/rehabsci_etds/12.
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Patient and caregiver education is recognized as a critical component of stroke rehabilitation and physical therapy practice yet the informational needs of stroke survivors and caregivers are largely unmet and optimal educational interventions need to be established. The objective of this dissertation was to develop a theory and model of “Rehabilitation Education for Caregivers and Patients” (RECAP) in the context of physical therapy and stroke rehabilitation, grounded in the experiences and perceptions of stroke survivors, their caregivers, and physical therapists.
Qualitative research methods with a novel grounded theory approach were used. Potential constructs of RECAP were identified from existing research. Next, semi-structured interviews were conducted with 13 stroke survivors and 12 caregivers from rural Appalachian Kentucky, a region with high incidence of stroke and lower levels of educational attainment. Lastly, 13 physical therapists, representing inpatient rehabilitation, outpatient, and home health, were recruited and participated in pre-interview reflection activities and interviews. Data analysis involved predetermined and emerging coding and a constant comparative method was employed. Verification strategies included self-reflective memos, analytic memos, peer debriefing, and triangulation.
The theory generated from this dissertation is: physical therapists continually assess the educational needs of stroke survivors and caregivers, to participate in dynamic educational interactions that involve the provision of comprehensive content, at a point in time, delivered through diverse teaching methods and skilled communication. This phenomenon is influenced by characteristics of the physical therapist and receiver (stroke survivor/caregiver) and occurs within the context of the physical therapist’s professional responsibility, the multidisciplinary team, a complex healthcare system, and the environmental/socio-cultural context. The RECAP theoretical model depicts the relationships between the core and encompassing constructs of the theory.
The RECAP theory and model presents a significant advancement in the study of patient and caregiver education in physical therapy in stroke rehabilitation. This research provides a springboard to inform future research, guide RECAP in stroke physical therapy practice, design optimal educational interventions, develop training tools for entry-level curriculum and practicing clinicians, and to potentially translate to the practice of patient and caregiver education for other rehabilitation professionals and patient populations.
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Burgsmüller, Mirja Maren [Verfasser]. "Revisionsrate, Komplikationen sowie postoperative Belastbarkeit der Patienten nach Implantation eines ReCap-Oberflächenersatzes am Hüftgelenk / Mirja Maren Burgsmüller." Köln : Deutsche Zentralbibliothek für Medizin, 2016. http://d-nb.info/1081788976/34.
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Sikorová, Lucie. "Určení prostorového tvaru stavební konstrukce pomocí laserového skenování." Master's thesis, Vysoké učení technické v Brně. Fakulta stavební, 2016. http://www.nusl.cz/ntk/nusl-390170.
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The aim of this work is to create a 3D models of the structures of data acquired by laser scanning. The tracking data are elaborated by software Scene and models were created in AutoCAD, Revit and SketchUP. The main output of this work are three models of the two structures.
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Martins, Alice Branquinho. "Avaliação da utilização de ligas de cobre-zinco (latão) em permutadores de calor que trabalham com água de resfriamento na UN-RECAP/PETROBRAS." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2009. http://hdl.handle.net/10183/18300.
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Os permutadores de calor são equipamentos projetados para realizar a transferência de energia térmica entre dois fluidos, utilizando-se, geralmente, materiais metálicos como meio condutor e água de resfriamento para absorver calor desses equipamentos em processos industriais. Esta água quando não devidamente tratada, apresenta uma série de inconvenientes como depósito em tubulações e tubos, ocasionando perda de carga, e corrosão com conseqüentes perfurações nos tubos. Este trabalho visa avaliar a utilização de ligas de cobre-zinco (latão) em tubos de permutadores de calor que trabalham com água de resfriamento na UN-RECAP. Foram levantadas curvas de polarização das ligas de tubos latão C44300, C44500 e C68700 utilizadas nos permutadores de calor da Unidade, utilizando-se como eletrólito a água de resfriamento de uma torre de resfriamento da Unidade, com preparação de superfície das amostras com e sem passivação prévia em solução do inibidor de corrosão. Os ensaios foram realizados à temperatura ambiente e à temperatura crítica dos permutadores de 60ºC. Para avaliação dos mecanismos de falhas dos permutadores de calor mais críticos da Unidade, foram utilizados: o histórico das análises físico-químicas da água de resfriamento, os resultados dos ensaios de IRIS, as curvas de polarização e o histórico de inspeção dos equipamentos. Os resultados das curvas de polarização das ligas de latão apresentaram dissolução ativa sem passivação para todas as ligas na temperatura ambiente e na temperatura crítica, com indicativo de passivação para a amostra C44300 com passivação prévia ensaiada a temperatura ambiente. O processo de dezincificação foi identificado nos processos de falha dos permutadores de calor em operação.Heat exchangers are equipment designed to accomplish the heat transfer between two fluids, using metallic materials as conductive middle and a cooling water to absorb heat in those equipments. This water, when not properly treated, presents a series of inconveniences as deposits on pipes, load loss and corrosion with consequent holes in the tubes. This study aims to evaluate the use of copper-zinc alloys (brasses) in tubes of heat exchangers that work with cooling water system of the UN-RECAP/PETROBRAS. There were lifted up polarization curves of brasses alloys tubes C44300, C44500 and C68700 used in heat exchangers being used cooling water as electrolyte, with samples surface preparation with and without prior passivation solution of a corrosion inhibitor. Tests were performed at room temperature and to the critical temperature at 60ºC (the critical temperature). In order to evaluate the failure mechanisms of the most critical heat exchangers, physical and chemical analysis, historical of cooling water, IRIS test results, polarization curves and inspection data reports were used. The results of polarization curves of brasses alloys showed active dissolution without passivation for all alloys at room temperature and critical temperature, with an indication of passivation of the sample with prior passivation tested at room temperature. The process of dealloying was identified in the process failures of heat exchangers in operation.
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Berisha, Suela. "L'apport des concepts du Web sémantique et normes associées aux échanges inter applicatifs dans un SI d'entreprise ou RECAP (Référentiels et connecteurs a priori)." Thesis, Lyon, INSA, 2011. http://www.theses.fr/2011ISAL0073.
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Le sujet de thèse a été traité dans le cadre d’un Système d’Information d’Entreprise (SIE) contenant un grand volume d’informations, très diverses, circulant au sein d’un groupe avec une cinquantaine de filiales. Initialement, les sources informatiques de chaque entité stockaient des données structurées, suivant des logiques métiers locales. Les descriptions de ces sources, destinées prioritairement aux informaticiens, se trouvent dans des documents textuels. Elles ne visaient pas le partage et la compréhension des systèmes par les différents types d’acteurs métiers qui fournissent le contenu. C’est également le cas des référentiels métiers, qui contiennent des connaissances métiers fondamentales. Ainsi, la localisation d’informations pertinentes, permettent un aiguillage et un accès aisé aux sources qui répondent aux attentes et aux contraintes des acteurs métiers, devient une problématique principale du SI. Depuis une décennie, la SNCF vise à répondre à cette problématique par une démarche stratégique de gouvernance inscrite dans les processus de l’urbanisme du SI. Nous sommes concernés par mise sous contrôle des référentiels de données métiers, la standardisation et la simplification des échanges inter applicatifs. Malgré cette démarche, les initiatives de partage d’informations et des connaissances métiers restent ponctuelles, fortement dépendantes des motivations personnelles (et non pas métiers) d’acteurs éclairés du SI. Notre objectif est de fournir une vue stratégique sur le partage des connaissances des différents types d’acteurs du SI en favorisant leur collaboration dans le contexte du déroulement d’un processus, ou d’une activité métier. Pour y parvenir, nous proposons une démarche pour la construction d’une couche du SI en s’appuyant sur de nouveaux concepts fonctionnels : le "référentiel de référentiels" et les "connecteurs a priori". Le premier correspond à un référentiel transverse par rapport à un périmètre métier. Le second correspond à des connexions logiques assurant une interopérabilité entre les applications qui ne sont pas conçues pour cohabiter. D’un point de vue technologique, le "référentiel des référentiels" prend ses fondements dans la modélisation et le stockage des connaissances par les démarches sémantiques. Les "connecteurs a priori" puisent leurs apports dans les Services Web Sémantiques. Le sujet de thèse développe des compétences comme les architectures middlewares orientées services, la modélisation sémantique de documents, la modélisation du SI et les techniques de Recherche d’Information (RI). Ce sont autant de sujets qui s’intègrent dans le projet scientifique de l’équipe DRIM (Distribution et Recherche d'Information Multimédia) du département « Données, Connaissances et Services » du laboratoire LIRIS (Laboratoire d’InfoRmatique en Images et Systèmes d’Informations), CNRS UMR 5205This thesis came about a very large and complex Enterprise Information System (EIS) containing a huge amount of information that moves through a company consisted of a main group and about fifty subsidiaries. At the beginning, computer science resources of each business unit stored structured data, according to a local business logic. The descriptions of these sources, intended first of all for computer specialists, were saved in text documents. They were not meant to help system sharing and understanding by different types of actors, who provide the business content. It's also true for the enterprise repositories, which contain fundamental business knowledge. Thus, finding the relevant information allowing fast and easy access to sources that meet the expectations and constraints of business actors, became a main problematic of IS. Since ten years, our company aims to address this problem by a strategic approach of IS governance included in the process of the IS planning. We are involved in the activity of business data repositories brought under control and information exchanges standardization and simplification between applications. Our goal is to provide a strategic view of knowledge sharing by promoting the IS actor collaboration in a business process context, or in business activity. To achieve this, we propose an approach for the construction of a layer of the IS based on new functional concepts: the "Reference Enterprise Repository (RER)" and " a priori connectors ". The first is about a transverse enterprise repository corresponding to a business context. The second is logical connections ensuring interoperability between applications that weren’t designed to coexist. From a technological point of view, the RER is based on semantic modelling and the knowledge representation. The "a priori connectors" involve integration technologies of Semantic Web Services. The thesis develops skills such as service-oriented middleware architectures, modelling semantics of documents, modelling IS and Information Retrieval (IR) techniques. All these subjects are part of the DRIM (Distribution and Multimedia Information Retrieval) team’s scientific project of the Department "Information, Knowledge and Services' at the LIRIS (Laboratoire d'Informatique en Images and Systems information), CNRS UMR 5205
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Lundström, Fredrik. "Utvärdering av miljön i området Torparängen med laserskanning." Thesis, Linnéuniversitetet, Institutionen för byggteknik (BY), 2017. http://urn.kb.se/resolve?urn=urn:nbn:se:lnu:diva-61967.
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Rapportens syfte är att få reda på om utvändig laserskanning av en byggnad är en lämplig metod att använda för byggbranschen. En laserskanning kommer göras av torpet Furutå, i området Toparängen, som ligger i Växjö. Laserskanningen har gjorts genom att tre uppställningar med skannern har genomförts. Denna data sammanfogas sedan i programmet Leica Cyclone. Sedan bearbetas modellen i Autodesk Recap och AutoCAD. Här skapas sedan 2D-ritningar av fasader och även en 3D-modell. Laserskanningen kommer även georefereras, vilket innebär att modellen flyttas från ett lokalt koordinatsystem till svenska referenssystemet SWEREF99. Studien visar på att laserskanning har ett brett användningsområde och med tillräckligt låg mätosäkerhet.
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Vanhook, Patricia M., and T. Hansen-Turton. "A Five-Year Recap of the Impact of the U.S. Institute of Medicine’s Report on the Future of Nursing in the U.S. and Its Implications for Nurses Globally." Digital Commons @ East Tennessee State University, 2016. https://dc.etsu.edu/etsu-works/7425.
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Mehmood, Rashid. "A Study of Reconfigurable Antennas as a Solution for Efficiency, Robustness, and Security of Wireless Systems." BYU ScholarsArchive, 2015. https://scholarsarchive.byu.edu/etd/5556.
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The reconfigurable aperture (RECAP) is a reconfigurable antenna consisting of a dense array of electronically controlled elements, which can be manipulated to support many antenna functions within a single architecture. RECAPs are explored herein as an enabling technology for future software defined and cognitive radio architectures, as well as compact wireless devices supporting many bands and services. First, the concept of a parasitic RECAP is developed and analyzed for various communication applications. This begins with the analysis of existing RECAP topologies (e.g. planar and parasitic) using a hybrid method combining full wave simulations and network analysis. Next, a performance versus complexity analysis is performed to assess the use of a parasitic RECAP for the most critical communications functions: pattern synthesis, MIMO communications and physical-layer wireless security. To verify simulation results, a prototype parasitic RECAP is also built and deployed in real propagation environments. Given the potential of adaptive and reconfigurable architectures for providing enhanced security, an idealized reconfigurable antenna is analyzed, resulting in the concept of secure array synthesis. The objective is to find optimal array beamforming for secure communication in the presence of a passive eavesdropper in a static line-of-sight (LOS) channel. The method is then extended to the case of multipath propagation environments. The problem is solved by casting it into the form of a semi-definite program, which can be solved with convex optimization. The method is general and can be applied to an arbitrary array topology with or without antenna mutual-coupling. Due to complexity of the problem, initial attention has been restricted to idealized reconfigurable antennas (smart antennas), where excitation amplitude and phase at each element can be controlled independently. Lastly, reconfigurable antennas are investigated as a solution to support the emerging application of over-the-air (OTA) testing in a low-cost and compact way, resulting in the concept of the reconfigurable over-the-air chamber (ROTAC). First, an idealized two-dimensional ROTAC is analyzed, revealing that the fading distribution, spatial correlation, frequency selectivity, and multipath angular spectrum can be controlled by proper specification of the random loads. Later, a prototype of ROTAC is built to study the fading statistics and angular characteristics of the multipath fields inside a practical chamber.
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Rydberg, Erik, and Andreas Johannesson. "Laserskanning av Södra Climate Arena." Thesis, Linnéuniversitetet, Institutionen för byggteknik (BY), 2016. http://urn.kb.se/resolve?urn=urn:nbn:se:lnu:diva-56461.
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Rapporten går ut på att se om laserskanning är en bra metod att använda sig av vid ombyggnation, tillbyggnad och renovering. För att få fram ett svar på detta har två takbalkar i Södra Climate Arena skannats av. Vid genomförandet användes en laserskanner från Leica av varianten P30. Försöket innehåller fyra stycken uppställningar som riktas mot sex stycken måltavlor. Informationen som fåtts ut från skanningen i form av en sektion i AutoCAD har jämförts med befintliga ritningar för att se om informationen från skanningen stämmer överens med ritningarna. I modellen som fåtts ut från försöket har också mätning och undersökning skett för att se hur bra det går att mäta i modellen och om det är något som fungerar lätt att använda sig av i praktiken. Arbetet går även ut på att få en god inblick i hur laserskannern Leica P30 fungerar.
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Schroeck, Christopher A. "A Reticulation of Skin-Applied Strain Sensors for Motion Capture." Cleveland State University / OhioLINK, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=csu1560294990047589.
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Lindholm, Tobias. "Energioptimering för Recip Strängnäs AB." Thesis, Mälardalen University, Department of Biology and Chemical Engineering, 2008. http://urn.kb.se/resolve?urn=urn:nbn:se:mdh:diva-591.
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Recip Strängnäs AB är ett miljöcertifierat företag som framställer och förpackar penicillin produkter. Företaget måste göra vissa reparationer på ett av ventilationssystemen. De tar tillfället i akt att gå igenom energiomsättningen i helhet då åtgärder redan måste göras. På grund av det ökade oljepriset och mer fokus på miljön är det intressant att byta ut denna energikälla som används idag, vilket är ånga som produceras genom eldning av olja. Den energikälla som är tänkt att ersätta ångan är fjärrvärme.
Flera processer som företaget använder sig av kräver ånga, men beräkningar visar att det bara behövs ca 400 MWh/år för att driva dessa processer. Då den totala energiomsättningen för Recip Strängnäs AB är kring 3100 MWh/år skulle det preliminärt innebära en besparing på 120 000 kr/år för att gå över till att använda fjärrvärme där det är möjligt istället för ånga.
Det hygienvatten som används på Recip Strängnäs AB kostar i dagsläget ca 76 000 kr/år att värma. En övergång till fjärrvärme skulle sänka den kostnaden till ca 66 000 kr/år. Tanken är dock att använda sig av båda kondensatvattnet, som samlas upp i kondensattanken, och den spillvärme som produceras av värmeväxlaren för ånga, destillationen och kompressorerna till att förvärma hygienvattnet. I denna rapport presenteras två alternativ på hur det kan lösas.
Även drifttiderna för de större fläktarna har setts över. Genom att ställa ner dem till mer anpassade tider kan elkostnaderna minskas med kring 87 000kr/år.
Det finns ytterligare möjligheter att sänka kostnaderna. Det är bland annat genom att ställa om kylkompressorn så att den använder mindre vatten och istället höjer temperaturen på det vatten som släpps ut. Det är möjligt att ställa dem på så vis att det går att använda det varma vattnet för uppvärmning av vissa processer. Den fungerar då som en värmepump.
Vid drageringen finns det ett utgående flöde av varmluft på drygt 40ºC som inte används till något. Genom att installera en värmepump mellan utgående och ingående lufttrumma är det möjligt att minska energiförbrukningen för drageringen med ca 50 %. Det visade sig dock att installationskostnaderna blev högre än priset på den sparade energin.
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Fuller, Charles E. Russell Douglas V. "A design rationale capture using REMAP/MM /." Monterey, Calif. : Springfield, Va. : Naval Postgraduate School ; Available from National Technical Information Service, 1994. http://handle.dtic.mil/100.2/ADA283534.
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Fuller, Charles E., and Douglas V. Russell. "A design rationale capture using REMAP/MM." Thesis, Monterey, California. Naval Postgraduate School, 1994. http://hdl.handle.net/10945/30937.
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Every year the Department of Defense (DoD) spends between 24 and 32 billion dollars on software alone, with maintenance costs comprising the majority of this figure. Recent studies have indicated that an effective solution to help curtail the large maintenance cost is by capturing the rationale which was used to create the systems requirements and designs, and using this information throughout the life cycle. This thesis explores the use of the REMAP/MM model for the purposes of capturing these rationale, and presents a detailed example of how REMAP/MM utilizes multimedia artifacts as tools in various systems development activities.
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DiCapua, Elisabeth. "Complexes of recA protein with DNA /." [S.l.] : [s.n.], 1986. http://e-collection.ethbib.ethz.ch/show?type=diss&nr=8212.
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Dale, Don. "Harvest The Water, Reap The Rewards." College of Agriculture, University of Arizona (Tucson, AZ), 1988. http://hdl.handle.net/10150/295556.
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Körner, Sven J. [Verfasser]. "RECAA - Werkzeugunterstützung in der Anforderungserhebung / Sven J. Körner." Karlsruhe : KIT Scientific Publishing, 2014. http://www.ksp.kit.edu.
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Preuil, Sébastien. "La mise en tourisme du patrimoine khmer de la province de Siem Reap au Cambodge : aide à la lutte contre la pauvreté ou facteur de paupérisation?" Versailles-St Quentin en Yvelines, 2014. http://www.theses.fr/2014VERS024S.
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Le Cambodge, petit pays d’Asie du Sud-Est correspond à l’une des nations les plus pauvres de la planète. Pourtant le pays demeure très riche sur le plan patrimonial, notamment dans la province de Siem Reap, berceau des temples d’Angkor. Ces vestiges archéologiques classés sur la liste du patrimoine mondial, fierté du peuple khmer attire chaque année près de 2 millions de visiteurs. Cette province du Cambodge est devenue alors en l’espace d’une vingtaine d’années la province la plus dynamique du pays sur le plan économique et attire des migrants en provenance des autres provinces du Cambodge. Pourtant malgré ce dynamisme, la province de Siem Reap demeure la plus pauvre du pays en termes de développement humain. Devons nous déduire que, dans le cas de pays en développement touchés par une pauvreté extrême, un territoire devenu subitement très attractif grâce à un secteur pourvoyeur d’emplois et susceptible de rapporter (pour certains) des salaires conséquents, engendre une misère plus grande encore que celle existant dans les provinces et les régions d’où proviennent les migrants ? Le tourisme de masse est-il responsable de ce phénomène de paupérisation ? Le tourisme durable peut-il renverser cette tendance ?Cambodia, is a small country in South East Asia and one of the poorest nations on earth. Yet the country is still very rich in terms of heritage, particularly in the province of Siem Reap, home of Angkor temples. These archaeological place is the pride of the Khmer people and UNESCO declared Angkor a world heritage site in 1994. Each year nearly 2 million tourists visit this part of Cambodia. After the end of Khmers rouges régime and civil war, Siem Reap province has become the most dynamic province in the country and attracts migrants from other provinces of Cambodia. Yet despite this dynamism, Siem Reap province is still the poorest in terms of human development. How can we explain this paradox? Mass tourism can be responsible about this phenomenon of extreme poverty? Sustainable tourism and responsible tourism can be an answer to reduce this poverty?
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Chen, Zhucheng. "Mechanism of homologous recombination : from crystal structures of RecA-single stranded DNA and RecA-double stranded DNA filaments /." Access full-text from WCMC, 2009. http://proquest.umi.com/pqdweb?did=1619205721&sid=8&Fmt=2&clientId=8424&RQT=309&VName=PQD.
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Thomas, Nicola Alison. "RecA expression and DNA damage in mycobacteria." Thesis, University College London (University of London), 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.299376.
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Gopaul, Krishna Kumar. "Transcription of the Mycobacterium tuberculosis recA gene." Thesis, University College London (University of London), 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.401763.
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Saladin, Adrien. "Macromolecular Docking : applications to the RecA nucleofilament." Paris 7, 2009. http://www.theses.fr/2009PA077098.
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Les protéines jouent un rôle central dans de nombreux processus cellulaire et peuvent intervenir dans de nombreuses interactions différentes, avec d'autres protéines, de l'ADN, des lipides, ou de petits ligands. La détermination de ces interactions est fondamentale pour pouvoir comprendre des processus biologiques majeurs et de nombreuses méthodes expérimentales ont été développées pour les caractériser. Cependant les méthodes expérimentales sont longues et coûteuses et les méthodes informatisées de prédictions d'interactions pourraient, à terme, fournir dans ce contexte une aide précieuse permettant de guider de futures expériences en biochimie et biologie moléculaire. Le développement logiciels d'amarrage est également un processus difficile mettant en jeu des cycles de conception d'algorithme, d'implémentation et de tests. Au cours de ma thèse, j'ai développé une librairie orientée objet pour favoriser et accélérer les étapes d'implémentation et de tests des méthodes d'amarrage. Cette librairie, programmée en C++ et interfacée avec le langage de script Python, a été utilisée pour mettre au point et tester de nouvelles méthodes appliquées à l'amarrage protéine-ADN et à l'amarrage multi-composants. Des programmes développés à l'aide de cette librairie sont actuellement appliqués à l'étude des modes d'amarrage de l'ADN au complexe RecA, responsable de la recombinaison homologue chez les bactériesProteins play a central role in various cellular processes with various interactions with other proteins, DNA, lipids or small ligands. Because the determination of these interactions is fundamental for understanding key biological processes, several experimental methods have been developed to characterize them. Experimental studies can take a long time and an expensive. Computational methods can therefore be of great help to guide future biochemical experiments. Development of docking software is a long process involving cycles of algorithm conception, programming and tests. During my thesis, I developed an object oriented library to help and speed-up development and tests of docking methods. This library was programmed in C++ with Python bindings, and has been used to test new methods applied to protein-DNA docking and multicomponent docking. Programs made with the help of this library are presently used to study the binding of DNA to the RecA complex, responsible of homologous recombination
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DUWAT, PATRICK. "Caracterisation du gene reca de lactococcus lactis." Paris 11, 1993. http://www.theses.fr/1993PA112106.
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Le developpement industriel des bacteries lactiques (lab) necessite une bonne comprehension des phenomenes de stabilite genetique et des reponses de ces bacteries aux agressions de l'environnement. Chez les procaryotes, un element clef de la recombinaison homologue et de la reponse a l'endommagement de l'adn est code par le gene reca. Nous avons developpe une methode basee sur l'amplification par polymerisation en chaine qui a conduit au clonage de 13 fragments internes de genes reca de bacteries a gram positif. Les donnees, obtenues par la comparaison de ces fragments avec les proteines reca precedemment caracterisees, suggerent qu'il existe des differences fonctionnelles entre les proteines reca provenant de bacteries a gram negatif et celles provenant de bacteries a gram positif. L'utilisation du fragment interne du gene reca de lactococcus lactis a permis le clonage et le sequencage de l'integralite de ce gene. L'analyse transcriptionnelle du gene reca a montre qu'il existe un couplage transcriptionnel entre le gene reca et le gene situe en amont codant pour la formamidopyrimidine-dna-glycosylase, une proteine de reparation de dommages oxydatifs au niveau de l'adn. La caracterisation de la souche reca#, obtenue par remplacement de l'allele sauvage par une copie inactivee du gene reca, a montre que cette souche est incapable de faire efficacement de la recombinaison homologue ou de reparer son adn. De plus, cette souche est aussi incapable de repondre efficacement a un stress oxydatif ou a un choc thermique. Ces resultats constituent la premiere caracterisation d'un gene reca de bacterie lactique. Ils suggerent que la premiere fonction de la proteine reca dans l. Lactis serait de permettre a la cellule de repondre efficacement aux stress. Les modeles permettant d'expliquer ce role important de reca sont presentes en fin de ce manuscrit
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Mah, Wayne. "Single molecule study of RecA recombinase enzyme activity." Thesis, McGill University, 2008. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=18743.
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Homologous recombination is an essential pathway in the repair of DNA damage during the DNA replication process. RecA protein promotes the central steps in homologous recombination, after coating single-stranded DNA (ssDNA), RecA carries out a pairing and strand exchange reaction involving homologous DNA. This research project aims to characterize RecA function in homologous recombination using single molecule tethered particle motion (TPM). Using TPM to observe RecA extension along DNA, the RecA extension rate on ssDNA was determined for the first time. The rate obtained for dsDNA was similar, implying that RecA polymerizes along only one strand of a DNA substrate. The nucleation behaviour of RecA on DNA was also obtained from the extension trace, confirming the hypothesis that rapid nucleation on ssDNA is pH independent, while nucleation on dsDNA is pH dependent. Several pilot single molecule experiments aimed at monitoring the pairing and strand exchange reaction in real time were attempted. Although these experiments were unsuccessful, successful ensemble biochemical analogues of these experiments proved the feasibility of the single molecule experiments. These attempts gave insights into possible factors hindering success and led to experimental suggestions essential to the success of future experiments.La recombinaison Homologue est un chemin essentiel dans la réparation de dommages d'ADN pendant le procédé de réplication d'ADN. La protéine de RecA promeut les étapes centrales dans la recombinaison homologue, après avoir revêtu ADN seul-abandonné (ssDNA), RecA exécute un mettre et la réaction d'échange de brin impliquant ADN homologue. Ce projet de recherche vise à caractériser la fonction de RecA dans la recombinaison homologue utilisant la molécule seule mouvement de particule attaché (TPM). TPM d'utilisation pour observer l'extension de RecA le long d'ADN, le taux d'extension de RecA sur ssDNA a été déterminé pour la première fois. Le taux obtenu pour dsDNA était similaire, impliquant ce RecA polymerizes le long de seulement un brin d'un substrat d'ADN. Le comportement de nucleation de RecA sur ADN a été aussi obtenu de la trace d'extension, confirmant l'hypothèse ce nucleation rapide sur ssDNA est indépendant du pH, pendant que nucleation sur dsDNA est dépendant du pH. Plusieurs pilote plusieurs expériences de molécule seules ont visé à contrôlant le mettre et la réaction d'échange de brin a été tentée en temps réel. Bien que ces expériences étaient les ensembles infructueuses et réussies analogues biochimiques de ces expériences ont prouvé la possibilité des expériences de molécule seules. Ces tentatives ont donné de l'aux perspicacités dans les facteurs possibles freinant le succès et a mené à l'élément essentiel de suggestions expérimental au succès d'expériences futures
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Vestavik, Øyvind. "REAP : Et system for rettighetsstyring i digitale bibliotek." Thesis, Norwegian University of Science and Technology, Department of Computer and Information Science, 2002. http://urn.kb.se/resolve?urn=urn:nbn:no:ntnu:diva-220.
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Denne hovedfagsoppgaven tar sikte på å vise at digitale bibliotek kan publisere intellektuelle arbeider i Internet i overenstemmelse med opphavsretten. Den foreslår en arkitektur/et paradigme for å behandle rettigheter ved publikasjon gjennom Digitale Bibliotek som innebærer digital rettighetshåndtering. Gjennom en analyse av opphavsrettslovgivning og internasjonale avtaler, digitale biblioteks behov og standarder og system for digitale rettighetshåndtering under utvikling fastlegges premissene for en slik arkitektur. Basert på denne analysen foreslås en arkitektur/paradigme for et system for digital rettighetsbehandling for digitale bibliotek. Som del av
denne oppgaven har det blitt implementert en prototype av et slikt system,og dette systemet er dokumentert i denne oppgaven.
This master thesis is aimed at demonstrating that intellectual property can be published in the Internet by digital libraries in accordance with copyright laws. It proposes an architecture/paradigm for managing rights when publishing information through Digital Libraries involving digital rights management. Through an analysis of copyright laws and international treaties, the needs of Digital Libraries and evolving standards and systems for Digital Rights Management the foundations for a such an architecture are identified. Based on this analysis an architecture/paradigm for a system for digital rights management in digital libraries is proposed. As part of this work a prototype of such a system has been implemented and is documented in this paper.
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Pinna, Massimo. "Veredas infinitas, Recep??o italiana de Grandes sert?es: veredas." Universidade Federal do Rio Grande do Norte, 2012. http://repositorio.ufrn.br:8080/jspui/handle/123456789/16364.
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Previous issue date: 2012-06-04L'obiettivo principale di questo lavoro ? valutare le difficolt? di comprensione e di identificazione nella ricezione da parte della cultura italiana dell'opera Grande sert?o: veredas, da quelle trovate, in modo pionieristico, dal traduttore italiano, a quelle che sono state percepite e indicate al momento della lettura dai critici, dagli accademici, dall'autore di questo lavoro e, soprattutto, dai lettori comuni, mostrando, allo stesso tempo, che i problemi avuti dagli italiani nella traduzione esistono, sotto certi aspetti, anche per i brasiliani urbani, poich? la dimensione linguistico-geografica presente nel romanzo ? cos? peculiare, che perfino molti lettori di lingua portoghese ignorano il mondo plasmato dal linguaggio di Guimar?es Rosa rivelando una esacerbazione della questione universale espressa nella formula "traduttori, traditori". Partendo da tutto ci?, abbiamo cercato di dimostrare che, sebbene la traduzione di Edoardo Bizzarri abbia raggiunto un eccellente risultato, l'opera rosiana, cos? come nella poesia e di pi? di qualsiasi altra narrativa, comporta, nel passaggio da un idioma all'altro, perdite irrimediabili, tanto relative all'armonia musicale e ritmica, quanto alla richezza semantica che si occulta nel testo originale
O objetivo principal deste trabalho ? avaliar as dificuldades de compreens?o e de identifica??o na recep??o pela cultura italiana da obra Grande sert?o: veredas, desde as encontradas de modo inaugural pelo tradutor italiano, como tamb?m as que foram sentidas e indicadas no momento de sua leitura pelos cr?ticos, acad?micos, pelo autor desta pesquisa, mas, sobretudo, pelos leitores comuns, mostrando, por outro lado, que os problemas encontrados pelos italianos na tradu??o existem, sob certos aspectos, para os brasileiros urbanos, pois a dimens?o lingu?stico-geogr?fica presente no romance ? t?o peculiar que at? mesmo muitos leitores de l?ngua portuguesa desconhecem o mundo moldado pela linguagem de Guimar?es Rosa o que revela um acirramento da quest?o universal expressa na f?rmula "traduttori, traditori". A partir da? tentamos evidenciar que, embora a tradu??o de Edoardo Bizzarri tenha alcan?ado excelente resultado, a obra rosiana, assim como na poesia e mais que em qualquer outra narrativa, imp?e, na passagem de um idioma para outro, perdas incontorn?veis, seja da harmonia musical e r?tmica, seja da riqueza sem?ntica que se oculta no texto original
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Moura, Marcos Fioravante de. "Quando a imagem recai sobre si mesma : nota sobre desenho." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2015. http://hdl.handle.net/10183/135392.
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Este trabalho analisa minha produção em desenho entre 2013 e 2015; tratando-se da observação, descrição e problematização do meu processo de criação e do levantamento de ideias e ações decorrentes de todo esse processo. O desenho, como prática artística autônoma e autorreferente, é investigado a partir de uma relação de suspensão através da transferência de imagens fotográficas de referência, procurando estabelecer possíveis correlações entre a imagem produzida e a imagem sendo produzida. Este trabalho apresenta-se, pois, como um diálogo entre as camadas do processo de criação e suas reverberações conceituais e poéticas acerca da imagem.This paper analyses my production in drawing between 2013 and 2015; dealing with the observation, description and questioning of my creation process and the lifting of ideas and actions resulting from this hole process. The drawing, as an autonomous and self-referential art practice, is investigated from a relation of suspension through the transfer of photographical images of reference, seeking to establish potential correlations between the produced image and the image being produced. This work presents itself, therefore, as a dialogue between the creative process layers and their conceptual and poetic reverberations about the image.
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Clark, Randy R. Jr. "Development, Evaluation and Improvement of Correlations for Interphase Friction in Gas-Liquid Vertical Upflow." Diss., Virginia Tech, 2015. http://hdl.handle.net/10919/56953.
Full textAbstract:
In this study, liquid-vapor vertical upflow has been research with the intent of finding an improved method of modelling the interphase friction in two-phase vertical flow in nuclear thermal-hydraulic codes. An improved method of modelling interphase friction should allow for better prediction of pressure gradient, void fraction and the phasic velocities.
Data has been acquired from several available published resources and analyzed to determine the interphase friction using a force balance between the liquid and vapor phases. Using the Buckingham Pi Theorem, a dimensionless interphase friction force was tested and refined before being compared against seven other dimensionless parameters. Three correlations have been developed that establish a dimensionless interphase friction force as a function of the Weber number, the Froude number and the mixture Froude number. Statistical analysis of the three correlations shows that the mixture Froude number correlation should be the most accurate correlation. The correlations have a weakness that makes them ineffective mostly for bubbly flow and some slug flow scenarios, while they should perform significantly better for annular flow cases.
Comparisons have been made against the interphase friction calculations published in the manuals of RELAP5/MOD2, RELAP5/MOD3.3, RELAP5-3D and TRACE. The findings have generally shown that the equations in the manuals provide very inaccurate approximations of the interphase friction compared to the interphase friction that was found via force balance. When analyzing the source code of RELAP5/MOD3.3, several differences were noticed between the source code and manual, which have been discussed. Calculations with the source code equations reveal that the source code provides a modestly improved prediction of the interphase friction force, but still has significant errors.
Despite the fact that the manual and source code equations indicate that RELAP5/MOD3.3 should perform poorly in modelling interphase friction, actual RELAP5/MOD3.3 model runs perform very well in predicting pressure gradient, void fraction, the liquid and vapor velocities and the interphase friction force. This is largely due to RELAP5/MOD3.3 being able to adjust parameters to converge to a solution that fits within the boundary conditions established in the input file.
Modifications to the RELAP5/MOD3.3 code were first made with the three correlations developed using dimensionless parameters, and were tested with data points that the RELAP5/MOD3.3 flow regime map had predicted would be annular flow. While the mixture Froude number correlation has been analyzed to be the most statistically accurate of the three correlations, it was found that the Weber number correlation performed best when implemented into RELAP5/MOD3.3. In a parametric study of the Weber number correlation, it performed optimally at 150% of the original correlation, improving upon the original RELAP model in almost every metric examined.
Additional investigations were performed with individual annular flow correlations that model specific physical parameters. Results with the annular flow physical models were inconclusive as no particular model provided a significant improvement over the original RELAP5/MOD3.3 model, and there was no clear indication that combining the models would provide significant improvement.Ph. D.
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Saladin, Adrien [Verfasser]. "Macromolecular Docking. Applications to the RecA nucleofilament / Adrien Saladin." Bremen : IRC-Library, Information Resource Center der Jacobs University Bremen, 2010. http://d-nb.info/1034989146/34.
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Knight, Kerry Amy Louise. "oraA - a gene which affects RecA in Escherichia coli." Thesis, University of Cambridge, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.624507.
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Muñoz, Daniela. "Parque inundable en Siem Reap tejedor de relaciones urbanas." Tesis, Universidad de Chile, 2007. http://repositorio.uchile.cl/handle/2250/101102.
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Este proyecto nace a raiz de un viaje de estudios del taller de 5° año “ville et patrimoine
en asie” de L’ Ecole Nationale Supérieure d’Architecture de Paris-Belleville (ENSAPB),
realizado en diciembre de 2006 a Siem Reap Camboya.
la finalidad del viaje de estudios fue comprender la conformación urbana de Siem Reap,
marcada profundamente por el agua (lluvias monzónicas que inundan ciudades, crecidas del lago mas grande de asia), comprender sus tejidos, zonas identitarias, conformación fisica y social, descubrir problemáticas y posibles soluciones en una ciudad del
país mas pobre, y con el potencial turístico mas fuerte del sudeste asiático.
Conocer Camboya marco intensamente mi forma de comprender el espacio, el flujo de
las aguas condiciona escalas, modos de vida, rigideces y flexibilidades en un enorme
neo_ecosistema natural humanizado, que genera microtopografías que se transforman
en espacios de creación de vida.
Entender la riqueza y heterogeneidad de los tramados relacionales camboyanos, tejidos entre si por un elemento común, el agua, es el motivo por el cual decido trabajar
en Camboya.
En mi estadía en Siem Reap encuentro un terreno marcado por esta piel colmada de
diversidad donde los tramados que podrian generarse entre los elementos identitarios
que conforman esta diversidad estan cortados por un vacio que se inunda casi la mitad
del año.
Reinterpretar el espacio urbano Camboyano entendiendo el arte de danzar con el agua,
que teje tramados relacionales, y generar un espacio público democrático_diverso es el
tema del proyecto que les presento.
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Rajan, Rakhi. "Structural and functional studies of the bacterial RECA protein." The Ohio State University, 2007. http://rave.ohiolink.edu/etdc/view?acc_num=osu1186676763.
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Oliveira, Lady Dayana Silva de. "Cinema itinerante no Rio Grande do Norte: aspectos da recep??o." Universidade Federal do Rio Grande do Norte, 2012. http://repositorio.ufrn.br:8080/jspui/handle/123456789/16411.
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Previous issue date: 2012-02-15In itinerant film projects made in Brazil, the unique experience of watching movies on the big screen is held in open spaces, through the establishment of a contemporary ritual, under which the presence of spectators is primordial. Considering this dynamic of the performance of itinerant cinema, the main objective of this research is to analyze the process of reception of the spectators of the sessions of Cine Sesi Cultural, conducted by the Social Service Industry - Sesi, in Rio Grande do Norte state. The body of research was composed by the audience of the movie sessions of the edition developed in 2010. Analyses were made from the look on the specific audience of open sessions of this cinema project throughout a case study. Theoretical authors of Latin American as Jesus Martin-Barbero, Guillermo Orozco, Eliseo Veron and Nestor Garcia Canclini, which have important theoretical basis for the analysis of research on the cinematographic reception of the spectators, were taken as a basis. In this discussion are associated with contributions from Brazilian authors as Roseli Paulino, Fernando Mascarello, Mauro Wilton Souza, Nilda Jacks and Carolina Escostesguy. Besides the reception study, the research focuses on aspects that relate to and explain the circumstances in which itinerant cinema emerges as an alternative exhibition, for example, the context of the exhibition of films in the country, lack of public policies in the audiovisual sector, and mainly the closing of movie theaters in the inner cities of the country and the consequent migration of these rooms to the malls. Seeking to reduce the existing gap in the studies of the reception of spectators to the cinema in the country, this research presents a deeper analysis of the reception of the public of the itinerant cinema as a contribution to an important database for the diagnosis of projects such as the Cine Sesi Cultural
Nos projetos de cinema itinerante realizados no pa?s, a experi?ncia ?nica de assistir a filmes na grande tela ? realizada em espa?os ao ar livre, com o estabelecimento de um ritual contempor?neo, em que a presen?a dos espectadores ? fundamental. Considerando esta din?mica de realiza??o do cinema itinerante, o objetivo principal desta pesquisa ? analisar o processo de recep??o dos espectadores das sess?es de cinema do projeto Cine Sesi Cultural, realizado pelo Servi?o Social da Ind?stria SESI, no Rio Grande do Norte. O corpus da pesquisa foi composto pelo p?blico das sess?es de cinema do projeto na edi??o desenvolvida no ano de 2010. As an?lises foram feitas a partir do olhar sobre o p?blico das sess?es abertas de filmes desse projeto atrav?s de um estudo de caso. O referencial te?rico consta de autores latino-americanos como J?sus Mart?n-Barbero, Guillermo Orozco, Eliseo Veron e Nestor Garc?a Canclini, que apresentam bases te?ricas importantes para as an?lises da pesquisa sobre a recep??o cinematogr?fica dos espectadores de cinema. Nesta discuss?o, est?o associadas tamb?m contribui??es de autores brasileiros como Roseli Paulino, Fernando Mascarello, Mauro Wilton Souza, Nilda Jacks e Carolina Escostesguy. Al?m do estudo de recep??o, h? debru?amento sobre aspectos que se relacionam com e explicitam as circunst?ncias em que o cinema itinerante surge como alternativa de exibi??o, como, por exemplo, o contexto da exibi??o de filmes no pa?s; a escassez de pol?ticas p?blicas no setor audiovisual; e, principalmente, o fechamento das salas de exibi??o de filmes nas cidades do interior do pa?s e a consequente migra??o dessas salas para os shopping centers. No intuito de diminuir a lacuna existente nos estudos sobre a recep??o de cinema no pa?s, esta pesquisa apresenta uma an?lise mais aprofundada da recep??o do p?blico do cinema itinerante com a perspectiva de contribuir para uma base de dados importante para um diagn?stico de projetos como o Cine Sesi Cultural
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Longo, Rosario Alessandro. "Dalla generazione di modelli 3D densi mediante TLS e fotogrammetria alla modellazione BIM." Master's thesis, Alma Mater Studiorum - Università di Bologna, 2017. http://amslaurea.unibo.it/13284/.
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La tesi tratta la ricerca di procedure che permettano di rilevare oggetti utilizzando il maggior numero di informazioni geometriche ottenibili da una nuvola di punti densa generata da un rilievo fotogrammetrico o da TLS realizzando un modello 3D importabile in ambiente FEM.
Il primo test si è eseguito su una piccola struttura, 1.2x0.5x0.2m, in modo da definire delle procedure di analisi ripetibili; la prima consente di passare dalla nuvola di punti “Cloud” all’oggetto solido “Solid” al modello agli elementi finiti “Fem” e per questo motivo è stata chiamata “metodo CSF”, mentre la seconda, che prevede di realizzare il modello della struttura con un software BIM è stata chiamata semplicemente “metodo BIM”. Una volta dimostrata la fattibilità della procedura la si è validata adottando come oggetto di studio un monumento storico di grandi dimensioni, l’Arco di Augusto di Rimini, confrontando i risultati ottenuti con quelli di altre tesi sulla medesima struttura, in particolare si è fatto riferimento a modelli FEM 2D e a modelli ottenuti da una nuvola di punti con i metodi CAD e con un software scientifico sviluppato al DICAM Cloud2FEM.
Sull’arco sono state eseguite due tipi di analisi, una lineare sotto peso proprio e una modale ottenendo risultati compatibili tra i vari metodi sia dal punto di vista degli spostamenti, 0.1-0.2mm, che delle frequenze naturali ma si osserva che le frequenze naturali del modello BIM sono più simili a quelle dei modelli generati da cloud rispetto al modello CAD. Il quarto modo di vibrare invece presenta differenze maggiori.
Il confronto con le frequenze naturali del modello FEM ha restituito differenze percentuali maggiori dovute alla natura 2D del modello e all’assenza della muratura limitrofa.
Si sono confrontate le tensioni normali dei modelli CSF e BIM con quelle ottenute dal modello FEM ottenendo differenze inferiori a 1.28 kg/cm2 per le tensioni normali verticali e sull’ordine 10-2 kg/cm2 per quelle orizzontali.
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Singh, Veena. "Evolutionary rearrangements in chloroplast genomes." Thesis, University of Cambridge, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.321027.
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Dutreix, Marie. "Caractérisation des activités de la protéine RecA impliquées dans la réparation de l'ADN et la mutagénèse." Paris 11, 1988. http://www.theses.fr/1988PA112296.
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La protéine RecA intervient dans la réparation des lésions de l’ADN (i) en clivant le répresseur LexA et en déréprimant les gènes SOS qui sont sous le contrôle de LexA, (ii) en clivant la protéine UmuD dont seule la forme clivée est active dans la réparation fautive, (iii) en participant directement à la recombinaison post-replicative et à la réparation fautive. Nous avons étudié l'importance des différentes activités de la protéine RecA dans la régulation de la réparation SOS grâce à l’emploi de mutants ponctuels. Dans les résultats rapportés dans le chapitre l, l'activité coprotéase de différents mutants recA a été estimée par leur capacité à induire des mutants du phage λ. Ces phages codent pour des répresseurs présentant des sensibilités différentes au clivage protéolytique. Nos résultats montrent que l'activité coprotéase dépend in vivo (i) du nombre de lésions et de l'aptitude de la cellule à éliminer ces lésions (ii) de la quantité de molécules RecA (iii) de la capacité de la protéine RecA à interagir avec les cofacteurs (NTP et ADN simple brin) pour former un complexe actif, (iv) de l'affinité du répresseur pour la protéine RecA. Le chapitre suivant concerne l'interaction de la protéine RecA avec les différentes protéines dont elle stimule le clivage: LexA, UmuD, λcI et Φ80cl. De nouveaux mutants recA ont été isolés. Leur étude montre que le changement d'un acide aminé affecte spécifiquement le clivage de certains répresseurs. En effet, le mutant recA1730 ne clive pas la protéine LexA, le mutant recA1734 ne clive pas UmuD et Φ80cl et le mutant recA430 clive partiellement LexA et pas du tout UmuD et λcI. Bien que les changements dans ces trois protéines ne concernent qu'un acide aminé, ils altèrent également l’activité de recombinaison de la protéine RecA. Deux classes de mutants altérés dans la recombinaison peuvent être distinguées : les mutants dont l'activité de recombinaison est partiellement restaurée par la surproduction de protéine RecA dans des bactéries LexA (Def) et ceux qui sont insensibles à cet effet. Le mutant recA1735 qui est décrit dans le 3ème chapitre présente un phénotype nouveau : la protéine RecA1735 amplifiée dans une bactérie LexA (Def) est létale. La protéine RacA1735 présente une activité coprotéase normale et une activité de recombinaison réduite. Un excès des protéines UmuCD impliquées dans la réparation fautive inhibe l'effet létal de l’amplification de la protéine RecA1735. Nous montrons que l'accumulation de protéine UmuCD réduit l'activité recombinatrice de la protéine RecA+. L'effet létal de la protéine RecA1735 pourrait être dû à des « accidents » au cours de la recombinaison qui causeraient des dommages irréversibles de l'ADN. Le dernier chapitre porte sur la comparaison des gènes PsiB portés par les plasmides F et R6-5. La présence d'un plasmide R6-5 inhibe l'activité coprotéase de la protéine RecA alors qu'aucune inhibition n'est détectée en présence du plasmide F. Bien que les protéines Psi B produites par les deux plasmides soient très semblables (seulement 4 acides diffèrent sur 152) seul le plasmide R6-5 Inhibe l'induction du système SOS. Nous montrons que cette activité anti-SOS est due à la production de protéine PsiB. J'ai résolu le paradoxe des différences d'inhibition par les plasmides F et R6-5 en montrant que les séquences de régulation du gène PsiB diffèrent dans une région de 71 nucléotides comprenant les sites -10 et -35 de reconnaissance de la RNA polymérase. L'activité des deux promoteurs a été mesurée grâce à leur fusion à un fragment du gène lacZ. Le promoteur du gène PsiB du plasmide R6-5 est trois fois plus actif que celui du plasmide F ce qui pourrait expliquer les différences d'inhibition observées.
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Lee, Andrew J. "The mechanism of RecA mediated DNA patterning interrogated by AFM." Thesis, University of Leeds, 2016. http://etheses.whiterose.ac.uk/13679/.
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Over recent years, advancements in bottom - up construction technologies are enabling the creation of heterogeneous and functional materials. These approaches offer the potential to surpass the physical limitations in traditional top - down micromachining. Of these, bionanotechnological approaches that harness the inherent molecular recognition and self-assembling properties of biological molecules - such as Deoxyribose nucleic acid (DNA) - are arguably the most promising. Over recent years, the field of DNA nanotechnology has advanced rapidly, enabling the creation of arbitrary structures in two and three dimensions. These substrates act as adapters enabling the arrangement of functional components at the nano-scale to be interfaced with the macro-scale world. One approach to spatially address DNA nano-architectures is to harness the sequence specific homologous recombination mechanism of the E.coli protein Recombinase A (RecA). This protein mediates the alignment of a supplied single stranded DNA (ssDNA) with a subject double stranded DNA (dsDNA) where homology is shared, making this method inherently programmable. Despite several successful demonstrations of the artificial application of RecA, the underlying mechanism which orchestrates this interaction remains widely debated. The lack of clear understanding surrounding this critical biological mechanism stems from the in-direct approaches taken to interrogate it, to date. In response to this, the work presented in this thesis, attempts to answer the open biological questions surrounding RecA. Here, recent advances in high speed atomic force microscopy (HSAFM) and high resolution Atomic force microscopy (AFM) - using rapid-force-curve imaging - are applied to directly interrogate the homology searching mechanism of RecA. When taken together, these structural and functional insights will inform the future development of RecA mediated patterning approaches within complex DNA topologies.
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Körner, Sven J. [Verfasser], and W. F. [Akademischer Betreuer] Tichy. "RECAA - Werkzeugunterstützung in der Anforderungserhebung / Sven J. Körner. Betreuer: W. F. Tichy." Karlsruhe : KIT-Bibliothek, 2014. http://d-nb.info/1048384918/34.
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Veronose, Cristina Gravina. "Estudos de tratabilidade dos efluentes da refinaria do Vale da Paraíba (REVAP)." reponame:Repositório Institucional da UCS, 2013. https://repositorio.ucs.br/handle/11338/810.
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A associação de biorreatores à membranas (MBR) é extremamente atrativa para o
tratamento de efluentes de refinaria de petróleo com o objetivo da remoção da matéria-orgânica e
de nitrogênio amoniacal. Esta tecnologia de tratamento é relativamente recente e encontra-se em
expansão. Os processos de remoção de matéria orgânica e de nitrogênio amoniacal foram
estudados em diferentes condições operacionais aplicadas à unidade experimental. Esta unidade
MBR operou 24 horas por dia, com dois módulos de membrana fibras ocas submersas de
ultrafiltração e fabricadas em PVDF, por um período de aproximadamente 18 meses. Durante
este período parâmetros como tamanho médio de flocos, produção de substâncias poliméricas
extracelulares (EPS), filtrabilidade do lodo e desempenho hidrodinâmico dos módulos de
membrana também foram avaliados. Os resultados obtidos foram divididos em três estudos que
contemplam as variações de carga orgânica volumétrica (COV), de carga nitrogenada
volumétrica (CNV) e de concentração de oxigênio dissolvido (OD) aplicadas. Durante o
primeiro estudo, realizou-se a avaliação da remoção de matéria orgânica em relação à sua
eficiência e aos parâmetros cinéticos através da COV, que variou de 0,78 a 3,16 kgDQO/m³.d.
Esta variação foi obtida através do aumento progressivo da vazão de efluente bruto da unidade.
O aumento crescente da COV e da CNV provocou a diminuição das eficiências de remoção de
matéria orgânica e nitrogênio amoniacal. Com base nos resultados deste estudo, verificou-se que
a unidade experimental deve ser operada com valores de relação alimento-microrganismo (A/M)
inferiores a 0,165 kgDQO/kgSSV.d e com valores de COV entre 0,35 e 0,7 kgDQO/m³.d para
que se obtenham eficiência de remoção de matéria orgânica superiores a 90%. Ainda, obteve-se
uma constante cinética de remoção de DQO para o reator anóxico de 0,025 L/mgSSV.d. e de
0,0138 L/mgSSV.d para o reator aeróbio. No segundo estudo, com ênfase na remoção de
nitrogênio amoniacal, variou-se novamente a vazão de efluente bruto, obtendo-se desta forma
CNV crescentes entre 0,087 e 0,169 kgN/m³.d. Foram obtidas eficiências de remoção de
nitrogênio amoniacal superiores a 97,5% para todas as CNV utilizadas. Durante este estudo,
dentre todos os parâmetros analisados a CNV apresentou a maior influência sobre esta eficiência
de remoção. Obteve-se uma velocidade máxima de consumo de nitrogênio amoniacal de 0,109
mgNH3-N/mgSSV.d e uma constante de saturação de 0,333 mg/L. O terceiro estudo, por sua vez,
avaliou a influência da concentração de OD na unidade experimental em quatro etapas distintas.
Durante a primeira etapa realizou-se, em um período de 2 meses, a diminuição progressiva da
concentração de OD no interior do reator aeróbio. Na segunda etapa, zerou-se a concentração de
OD no reator aeróbio e realizou-se a injeção de nitrogênio gasoso no tanque de membranas por
um período de quatro dias. Na terceira etapa foram realizados dois eventos de 24 horas de
duração na ausência de OD e fluxo de nitrogênio gasoso no tanque de membranas. Por fim,
durante a quarta etapa os dois eventos apresentaram duração de 2 horas, sendo o primeiro na
ausência de OD e com fluxo de nitrogênio e o segundo na ausência de OD e sem fluxo de gás no
tanque de membranas. Os resultados obtidos neste último estudo apresentarem algumas
divergências em relação à concentração e composição das EPS, à filtrabilidade e ao tamanho
médio de flocos. Entretanto, em geral, verificou-se que a remoção de nitrogênio amoniacal
diminuiu na ausência de OD e que esta eficiência é mais afetada pela diminuição e/ou ausência
de OD do que a remoção de matéria orgânica. As substâncias predominantes na composição das
frações solúveis e fracamente ligadas de EPS presente na unidade foram os ácidos húmicos. No
entanto, a fração fortemente ligada não apresentou predominância clara. O desempenho
hidrodinâmico dos módulos de membranas não foi afetado pela diminuição da concentração de
OD e pela utilização de fluxo de nitrogênio gasoso no tanque de membranas.Submitted by Marcelo Teixeira (mvteixeira@ucs.br) on 2014-07-10T16:26:59Z No. of bitstreams: 1 Dissertacao Cristina Gravina Veronese.pdf: 9043084 bytes, checksum: ef42eeab9bfd1aa382a15155c364d7d3 (MD5)
Made available in DSpace on 2014-07-10T16:26:59Z (GMT). No. of bitstreams: 1 Dissertacao Cristina Gravina Veronese.pdf: 9043084 bytes, checksum: ef42eeab9bfd1aa382a15155c364d7d3 (MD5)
Petrobrás
The association of bioreactors and membranes (MBR) is extremely attractive for the treatment of oil refinery wastewater aiming the removal of organic materials and ammonia. This treatment technology is relatively recent and is in continuos development. The removal efficiencies of organic materials and ammonia were studied at different operational conditions applied to the experimental unit. This MBR unit operated 24 hours per day, with two ultrafiltration hollow fiber membrane modules made of PVDF, for a period of approximately 18 months. During this period parameters such as mean floc size value, production of extracellular polymeric substances (EPS), sludge filterability, and hydrodynamic performance of the membrane modules were also evaluated. The results obtained were divided in three studies that contemplate the variations on the volumetric organic loading rate (VOLR), on the volumetric nitrogen loading rate (VNLR) and on the dissolved oxygen (DO) concentration applied. During the first study, an evaluation of the removal of the organic materials was held considering the efficiency and the kinetics parameters through the VOLR, that ranged from 0.78 to 3.16 kgCOD/m³.d. This oscillation was possible through the progressive increase of the wastewater flowrate. The increase of the VOLR caused the decrease of the removal efficiencies of organic materials and ammonia. According to the obtained results, it was verified that the experimental unit should be operated with values of food-to-microorganism (F/M) below 0.165 kgCOD/kgVSS.d and with values of VOLR between 0.35 and 0.7 kgCOD/m³.d in order to obtain removals efficiencies of organic materials higher than 90%. Still, a kinetic constant of COD removal of 0.025 L/mgVSS.d for the anoxic reactor, and a kinetic constant of 0.0138 L/mgVSS.d for the aerobic reactor were obtained. On the second study, with emphasis on the removal of ammonia, the wastewater flowrate was varied again, obtaining an increasing VNLR from 0.087 to 0.169 kgN/m³.d. All the removal efficiencies of ammonia obtained were superior to 97.5%. During this study, among all the analyzed parameters, the VNLR presented the greatest influence on this removal efficiency. Still, it was obtained a maximum ammonia consumption rate of 0.109 mgNH3-N/mgVSS.d and a saturation constant of 0.333 mg/L. The third study, on the other hand, evaluated the influence of the DO concentration inside the experimental unit on four different stages. During the first stage, through a period of 2 months, the progressive decrease of the DO concentration was done inside the aerobic reactor. On the second stage the experimental unit operated with the absence of DO and with the injection of nitrogen gas inside the membrane tank for a period of four days. On the third stage two different events with DO absence and the injection of nitrogen gas were performed for 24 hours. And at last, on the fourth stage the first event lasted for 2 hours without oxygen and with nitrogen gas and the second event lasted for 2 hours without oxygen and gas injection on the membrane tank. The results obtained on this last study presented some differences relative to the concentration and composition of EPS, to the sludge filterability and to the mean floc size value. However, in general, it was verified that the removal efficiency of ammonia decreased with the DO absence and that this efficiency was more affected by this absence than the removal of organic materials. The main substances on the composition of the soluble and slightly connected fractions of EPS on the experimental unit were the humic acids. But, the strongly connected fraction did not have a main substance of the composition. The hydrodynamic performance of the membrane modules were not affected by the decrease of DO concentration and for the injection of nitrogen gas inside the membrane tank.
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Koller, Luiz Antonio Monza. "Orientação para o mercado na refinaria REFAP S. A. : um estudo exploratório." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2002. http://hdl.handle.net/10183/3339.
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A necessidade de adoção de ações de marketing como integrantes das estratégias maiores das empresas encontra grande respaldo na literatura de administração. Estudos recentes demonstram que a prática de ações de marketing e, em particular, a opção estratégica da orientação para o mercado, está associada a performances empresariais superiores, satisfação e comprometimento dos funcionários. No Brasil, é crescente o número de estudos acadêmicos que exploram o tema da orientação para o mercado, contribuindo para a verificação de sua aplicabilidade em países outros que os Estados Unidos, sua origem. Esses trabalhos têm contribuído para ampliar a impleme ntação dos conceitos de marketing nas organizações brasileiras. Esta pesquisa aborda a verificação da existência de elementos que apontem uma orientação para o mercado na Refinaria de Petróleo REFAP S.A., que tem a Petróleo Brasileiro S.A. – Petrobras – como sua principal acionista. Para a sua execução foi utilizada metodologia do tipo exploratória e qualitativa, adotando-se um roteiro de entrevistas baseado no questionário elaborado pelos autores americanos Kohli, Jaworski e Kumar, a escala MARKOR. Através dessa escala, foram feitas entrevistas com representantes dos principais níveis gerenciais da refinaria, para investigar a existência de elementos ligados à Geração e Disseminação da Inteligência de Mercado, bem como as respostas da refinaria à inteligência gerada. Foram realizadas 21 entrevistas, envolvendo o Diretor-Presidente da empresa, todos os diretores, diversos gerentes e um coordenador de turno, entre os meses de janeiro e março de 2002. As respostas obtidas revelam a existência de elementos que apontam para uma orientação para o mercado, sem contudo ter sido observada uma opção estratégica específica da empresa dentro dessa área do marketing.
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Willumsen, Fransson Sara. "Cellular localization of the blood-brain barrier in the brainstem: Area postrema and nucleus tractus solitarius." Thesis, Uppsala University, Department of Medical Biochemistry and Microbiology, 2008. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-9070.
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The blood-brain barrier regulates the transport into the brain and protects the central nerve system (CNS) from toxics substances. However some areas of the brain, called circumventricular organs (CVO), lack the blood-brain barrier. One of these is area postrema (AP), which is located in the brainstem immediately adjacent to the nucleus tractus solitarius (NTS). These two areas together regulate autonomic behaviours such as food intake, and also make up the vomiting center.
The hormones leptin and ghrelin, which regulate food intake, are too big to pass the blood-brain barrier, but have receptors in NTS.
In this study we used immunohistochemistry to obtain a detailed map of the different components of the blood-brain barrier in AP and NTS.
The results suggest that there is a barrier that prevents diffusion of substances from AP into NTS. However, there seems to be some vessels in NTS that have a weaker or no barrier characteristics. These vessels could provide an entrance for peripheral substances to neurons in NTS.
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Clark, Randy Raymond. "Modeling Two-Phase Flow in the Downcomer of a Once-Through Steam Generator using RELAP5/MOD2." Thesis, Virginia Tech, 2011. http://hdl.handle.net/10919/76861.
Full textAbstract:
The purpose of this study is to develop an accurate model of the downcomer of the once-through steam generator (OTSG) developed by Babcock & Wilcox, using RELAP5/MOD2. While the physical model can be easily developed, several parameters are left to be adjusted to optimally model the downcomer and match data that was retrieved in a first-of-a-kind (FOAK) study conducted at Oconee Unit I in Oconee, South Carolina. Once the best-fit set of parameters has been determined, then the model must be tested for power levels exceeding that for which the steam generator was originally designed, so as to determine the power level at which a phenomenon known as flood-back becomes a concern.
All known previous studies that have been conducted using RELAP5/MOD2 have shown that RELAP over-predicts interphase friction. However, all of those studies focused on heated two-phase upflow, whereas the downcomer is modeled as adiabatic two-phase downflow. In this study, it is found that the original slug drag model for RELAP5/MOD2 developed by Idaho National Engineering Laboratory (INEL) under-predicts the interphase friction between the liquid and vapor phase within the downcomer. Using a modified version of the original slug drag model created by Babcock & Wilcox (B&W), an optimum multiplier is found for each power level. An increase of 1181% in interphase friction over the INEL slug drag model, which equals an increase of 4347% for the default B&W model provides the most accurate results for all power levels studied.
Emphasis is also placed on modeling the orifice plate of the OTSG downcomer which has been added to stabilize pressure fluctuations between the downcomer and tube bundle of the OTSG. While several different schemes are explored for modeling the orifice plate, a branch connection with an inlet area 14.22% of that of the downcomer is used to model the orifice plate along with the volume that transitions the two-phase downflow to horizontal flow into the tube nest of the OTSG.
Power levels exceeding that for which the steam generator was designed are tested in RELAP using the slug drag multiplier to determine at which power level a liquid level would occur and would flood-back become a concern. In this study, it is determined that a liquid level would form at 135% power and that at any higher power level, flood-back would be of concern for any user of the steam generator.Master of Science
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Mustard, Julie Ann. "A genetic analysis of RecA-LexA protein interactions in Escherichia coli." Diss., The University of Arizona, 1998. http://hdl.handle.net/10150/282594.
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The RecA protein of Escherichia coli is a small protein involved in many important functions including homologous recombination, mutagenic repair, regulation of the SOS system, and prophage induction. In normally growing cells, RecA is in an inactive form. However, when cells are subjected to DNA damage, RecA forms a helical filament with single stranded DNA and ATP. This ternary complex is the activated form of RecA. A key step in the regulation of several of these processes is the RecA mediated cleavage of different proteins. However, RecA is not a classic protease, but instead causes these proteins to undergo autodigestion. The main goal of this research was to investigate the role that RecA plays in cleavage by determining what residues in RecA interact with the cleavage substrates LexA, UmuD and λ CI. A possible model for the binding of the cleavage substrates in the cleft formed between two adjacent RecA monomers in an activated filament has been proposed. Site-directed mutagenesis was used to change residues in RecA that map to the cleft. An analysis of previously characterized recA mutants also suggested other regions of RecA that might interact with the cleavage substrates. Site-directed mutagenesis was used to also change these residues. Candidate residues were changed to alanine in order to reduce side chain contacts while minimizing perturbation of protein folding. The RecA mutant proteins were then characterized for ability to do recombination and DNA repair, and were examined for ability to mediate the cleavage of LexA, UmuD and λ CI. Several mutants showed some defects in cleavage of LexA or λ CI, while being proficient for other RecA functions. The fact that these mutants are selectively defective for cleavage of one protein, but not the others, suggests they can form activated filaments. Most of the mutations that differentially affected cleavage of LexA or λ CI mapped outside of the cleft region. This result suggests that the cleavage substrates do not bind in the cleft, but instead that the cleavable proteins may bind in the groove formed between turns of the RecA filament.
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Nair, Shamila. "Identification & molecular characterisation of a novel recA from Mycobacterium tuberculosis." Doctoral thesis, University of Cape Town, 1993. http://hdl.handle.net/11427/25659.
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Gustavsson, Niklas. "Create, maintain and reap the rewards of a social media strategy." Thesis, KTH, Skolan för datavetenskap och kommunikation (CSC), 2017. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-208998.
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This report analyzes the effects that social media marketing has on companies who choose to invest in a social media strategy. Previous research has looked at separate parts of a social media strategy, but no holistic view of the entire life cycle of a social media strategy has been created. Further, this report looks at what companies who choose to invest in a strategy gain from it in terms of different sources of business value. Advertising over digital media has increasingly taken market shares from more traditional advertising such as print, radio and TV. A large portion of this digital advertising is done through social media in the form of directing content at a specific target audience using the various social media platforms marketing algorithms. This increase in digital advertising is coupled by more companies choosing to create a social media presence. However, often have these social media presences failed to net any results due to a lack of understanding of the social media environment and how the consumers use the platforms. A case study was conducted on Marvelous, a Nordic digital agency specializing in social media. The case study also analyzed one of Marvelous’ clients who has invested in a social media strategy through them. The reason for this case study was to see how social media marketing is done in practice by a leading company within the industry. To complement the case study and get a less nuanced view of the development of a strategy and the results of it, qualitative interviews were held with other industry professionals who have worked with social media for several years. The results show that the life cycle of a social media strategy is made up of six stages: the pre-assessment stage, the strategy creation stage, the basics & hypotheses stage, the maturation & capitalization stage, the short-term evaluation stage and lastly the long-term evaluation stage. These different stages help companies in creating a strategy to guide social media efforts sustainably over a long period, leading to economical sustainability of the companies through granting advantages such as increased brand strength, increased sales and reaching a new consumer basis amongst other advantages. These advantages are gained while simultaneously strengthening the bond between consumer and company through the two-way communication that social media has enabled.Denna rapport analyserar effekten som marknadsföring via sociala medier har på företag som bestämt sig för att investera i en social mediestrategi. Tidigare forskning har utforskat olika delar i en social mediestrategi, men ingen holistisk vy av hela livscykeln har skapats. Vidare så tittar denna rapport på vad företag som har investerat i en strategi får ut av det i form av olika källor av affärsvärde. Marknadsföring över digitala medier har ökat och fortsatt tagit marknadsandelar från mer traditionella källor såsom print, radio och TV. En stor del av denna digitala marknadsföring görs via sociala medier genom att rikta ett budskap mot en specifik målgrupp med hjälp av de olika sociala plattformarnas marknadsföringsalgoritmer. Denna ökning av reklam via digitala medier syns även i form av att fler företag väljer att skapa en närvaro på sociala medier. Däremot så misslyckas ofta denna sociala närvaro att gynna företag på grund utav en brist på kunskap om det sociala medielandskapet och hur kunder använder dessa plattformar. En fallstudie gjordes på Marvelous, en nordisk digitalbyrå som specialiserar sig inom sociala medier. I denna fallstudie undersöktes även en av Marvelous kunder som har investerat i en social mediestrategi via dem. Anledningen till denna fallstudie var att se hur denna marknadsföring görs i praktiken av ett ledande företag inom industrin. För att komplettera fallstudien och få en mindre nyanserad bild av utveckling av en strategi och dess resultat så utfördes även intervjuer med andra experter inom industrin som har arbetat med sociala medier i ett flertal år. Resultaten visar att en livscykel för en social mediestrategi är uppgjord av sex skeden: förhandsbedömning, strategiskapande, etablering och hypotesprovning, mognads- och kapitalisering, kortsiktiga utvärderingar och slutligen långsiktiga utvärderingar. Dessa skeden hjälper företag att skapa en strategi för att vägvisa deras insatser på sociala medier hållbart över en lång period vilket kan leda till ekonomisk hållbarhet för företagen. Detta genom fördelar såsom ökad varumärkesstyrka, högre försäljningsgrad, att nå ut till en ny kundbas och andra fördelar därtill. Dessa fördelar fås medan företagen samtidigt stärker bandet mellan kund och företag genom den tvåvägskommunikation som sociala medier har möjliggjort.
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SOARES, Alexandre de Souza. "Estudo do acidente com perda de refrigerante de um reator PWR através de um simulador de escopo compelto e do código computacional RELAP." Instituto de Engenharia Nuclear, 2014. http://hdl.handle.net/ien/867.
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Submitted by Almir Azevedo (barbio1313@gmail.com) on 2015-01-06T14:40:18Z
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O presente trabalho propões um estudo de um acidente com perda de refrigerante de um reator PWR através de um Simulador de Escopo Completo e do código computacional RELAP. Para tal, foi considerado um acidente com perda de refrigerante com área de quebra de 160 cm2 na perna fria do circuito 20 do sistema de refrigeração do reator da planta da Usina Nuclear de Angra 2, com o reator operando em condições estacionária, a 100% de potência. Foi admitido ainda, que ocorreu simultaneamente a perda de Suprimento Externo de Energia Elétrica e que a disponibilidade do Sistema de Refrigeração de Emergência do Núcleo não era plena. Os resultados obtidos apresentam-se bastante relevantes e com possibilidade de serem usados no planejamento de atividades futuras, visto que a construção de Angra 3 se apresenta em andamento e se assemelha a Angra 2.
The present paper porposes a study of a loss of coolant accident of a PWR reactor through a Full Scope Simulator and computational code RELAP. To this end, it considered a loss of coolant accident with 160 cm2 breaking area in cold leg of 20 circuit of the reactor cooling system of nuclear power plant Angra 2, with the reactor operating in stationary condition, to 100% power. It considered that occurred at the same time the loss of External Power Supply and the availability of emergency cooling system was not full. The results obtained are quite relevant and with the possibility of being used in the planning of future activities, given that the construction of Angra 3 is underway and resembles the Angra 2.
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Leite, Wellington Claiton. "Caracterização Funcional e Determinação da Estrutura Tridimensional por Cristalografia de Raios X da Proteína RecA de Herbaspirillum seropedicae." UNIVERSIDADE ESTADUAL DE PONTA GROSSA, 2016. http://tede2.uepg.br/jspui/handle/prefix/863.
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Previous issue date: 2016-09-06Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
The bacterial RecA protein plays a role in the complex system of DNA damage repair. In the presence of ATP, RecA proteins polymerize onto single-strand DNA (ssDNA) as righthanded helical nucleoprotein laments, and catalyze strand exchange reaction between the ssDNA and homologous double-strand DNA (dsDNA) molecules. These activities are supported or stimulated by accessory proteins, as the single-stranded binding protein (SSB).Here, we report a functional and structural characterization of the Herbaspirillum seropedicae RecA protein (HsRecA).We report the crystal structure of HsRecA-ADP/ATP complex to 1.7 Å of atomic resolution. HsRecA protein contains a small N-terminal domain, a central core ATPase domain and a large C-terminal domain, similarly to homologous RecA proteins. Comparative structural analysis showed that the N-terminal polymerization motif of archaeal and eukaryotic RecA family proteins are also present in bacterial RecAs. The bacterial polymerization motif contains the sequence SV/IMR/KLG which interacts with the core ATPase domain residues DNLLLV/CS. In the inactive RecA, it is a loop - strand interaction, respectively, while in the active RecA it becomes a dyad strand. In both RecA forms, the polymerization motif seems to stabilize the subunitsubunit interface by hydrophobic interactions. The methionine of this motif may play an important role in the stability and formation of a right-handed helical nucleoprotein lament. The ATPase activity and the structure of the nucleoprotein lament of HsRecA and Escherichia coli RecA (EcRecA) were analyzed in the presence and absence of SSB. When SSB was added after RecA+ssDNA, HsRecA and EcRecA showed similar ATPase activity and nucleolament structure. However, when SSB was either not included or it was added before RecA+ssDNA, the HsRecA showed higher ATPase activity and formed longer nucleoprotein laments than EcRecA. Thus, HsRecA protein is more ecient at displacing SSB from ssDNA than EcRecA protein. HsRecA promoted DNA exchange more eficiently: a greater yield of nicked circular products were obtained in a shorter time. Reconstruction of electrostatic potential from the hexameric structure of HsRecAADP/ ATP revealed a high positive charge along the inner side, which is consistent with the fact that ssDNA binds inside the filament. It may explain the enhance capacity of HsRecA protein to bind ssDNA, forming a contiguous nucleoprotein filament, displace SSB and promote eficiently the DNA strand exchange reaction. Keywords: RecA, Crystallography, RecA nucleoprotein filament, ATPase activity, DNA strand exchange, crystal structure, structural analysis.
A proteína RecA bacteriana desempenha um importante papel no complexo sistema de reparo de danos ao DNA. Na presença de ATP, a proteína RecA se auto-polimeriza sobre o DNA simples ta (ssDNA) (do inglês single-strand DNA (ssDNA)) como um lamento de nucleoproteína helicoidal, cataliza a reação de troca de fitas entre as moléculas ssDNA e a ta de DNA dupla fita homóloga (dsDNA) (do inglês double-strand DNA (dsDNA)). Estas atividades são suportadas ou estimuladas por proteínas acessórias, como a proteína ligadora de ssDNA SSB (do inglês single-stranded binding protein (SSB)). Neste trabalho é apresentado a caracterização estrutural e funcional da proteína RecA da bactéria Herbaspirillum seropedicae. A estrutura tridimensional do complexo HsRecA-ADP/ATP foi resolvida numa resolução 1,7 Å. A estrutura monomérica da proteína HsRecA consiste em um pequeno domínio N-terminal, um domínio central contendo um sitío ATPásico e e um grande domínio C-terminal, similar com proteínas RecAs homólogas. Análises estruturais comparativas mostraram que o motivo de polimerização da região N-terminal de proteí- nas da familia RecA que incluem archaea e eucariotos, também está presente na proteína RecA bacteriana. O motivo de polimerização da região N-terminal de bactérias contêm a sequência de resíduos (Serina, Valina ou Isoleucina, Metionina, Arginina ou Lisina, Leucina, Glicina) que interage com a sequência de resíduos do core ATPásico (Aspartato, Asparagina, Leucina, Leucina, Leucina, Valina, Cisteína, Serina). Na proteína RecA inativa esta interação é do tipo loop - strand, respectivamente, enquanto na proteína RecA ativa essa interação se torna uma dupla -strand. Em ambas formas da RecA, o motivo de polimerização parece estabilizar a interface subunidade-subunidade por interações hidrofóbicas. No motivo N-terminal a presença de uma Metionina altamente conservada talvez desempenha um importante papel na estabilidade e formação do lamento de nucleoproteína. A atividade ATPásica e a estrutura do lamento de nucleoproteína da proteína HsRecA e da Escherichia coli RecA (EcRecA) foram analisadas na presença e ausência da proteína SSB. Quando a SSB foi adicionada após RecA+ssDNA, as proteínas HsRecA e EcRecA mostraram similar atividade ATPásica e estrutura de nucleo lamento. Entretanto, quando a SSB não estava incluída ou quando adicionada anteriormente a adição RecA+ssDNA, a proteína HsRecA mostrou maior atividade ATPásica e formou maiores lamentos de nucleoproteína que a proteína EcRecA. Ainda, a proteína HsRecA é mais eficiente em deslocar a SSB do ssDNA que a proteína EcRecA. A proteína HsRecA também promove a reação de troca de fitas mais eficientemente: uma maior quantidade de produtos duplex substrato convertido em duplex circular foram obtidos em um curto intervalo de tempo. A reconstrução do potencial eletrostático da estrutura hexamérica da proteína HsRecA revelou uma maior densidade de cargas positivas no seu interior, que é consistente com o fato que o ssDNA ligar-se internamente ao filamento hexamérico. Isto talvez possa explicar capacidade melhorada da proteína HsRecA ligar-se ao ssDNA, formando um continuo filamento de nucleoproteína, deslocando a SSB e ainda promovendo de forma eficiente a reação de trocas de fitas.
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Martinelli, Joseph A. "An X11 graphical interface for the REpresentation and MAintenance of Process Knowledge (REMAP) model /." Monterey, Calif. : Springfield, Va. : Naval Postgraduate School ; Available from the National Technical Information Service, 1993. http://handle.dtic.mil/100.2/ADA273169.
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Martinelli, Joseph Anthony. "An X11 graphical interface for the REpresentation and MAintenance of Process Knowledge (REMAP) model." Thesis, Monterey, California. Naval Postgraduate School, 1993. http://hdl.handle.net/10945/39975.
Full textAbstract:
Approved for public release; distribution is unlimited.The REpresentation and MAintenance of Process knowledge (REMAP) model provides support to various stakeholders involved in software projects by capturing the history of design decisions. This knowledge can assist the Department of Defense (DoD) in driving down the development and maintenance costs of large scale software systems. It is extremely important to have user friendly mechanisms to aid in the use of the REMAP model. This thesis implements a graphical user interface (GUI) under X11 Windows using the Andrew Toolkit. This implementation facilitates the instantiation, incremental modification, and ad-hoc querying of REMAP model primitives.
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Sandri, Tammie Caruse Faria. "Teoria geral da imagem e a produ??o de sentidos : modelo aplicado ? recep??o." Pontif?cia Universidade Cat?lica do Rio Grande do Sul, 2016. http://tede2.pucrs.br/tede2/handle/tede/7119.
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Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES
The General Theory of Image, by Justo Villafa?e and Norberto M?nguez, fits this investigative clipping to propose a path of analysis that note what is most essential in a visual narrative: its compositional elements and meanings that their arrangements evoke. The thesis sees the general theory of image as a basis theory for analysis of any visual discourse and adds that the study of meaning is only completed if contemplate the reception, in addition to observing the field of production, due to reciprocal nature of the communication process. The proposal echoes in the common question to many receivers in front of a picture: only am I seeing this? This doubt also affects many analysts image and refers to what is most basic in an image: its structural aspects, mentioned by the Theory. With a applicability of the General Theory of Image to the study of meaning production and reception in visual discourse, the thesis discusses the validity of the theory and believes that the structures forms the patterns, which forms culture. Exploratory research, from the proposed model, examines concrete experiences with volunteers receptors on the effects of meaning in the interlocution between the produced and the reception of television program scenario Palavras Cruzadas, covers of Vogue and Times magazines and ads of WWF and Panasonic. The results show the contributions that the cross analysis has to visual studies and to the improvement of professional practices in the area.
A Teoria Geral da Imagem, de Justo Villafa?e e Norberto M?nguez, prop?e um caminho de an?lise que observa o que h? de mais essencial numa narrativa visual: seus elementos compositivos e as significa??es que seus arranjos evocam. A tese a vislumbra como teoria de base para an?lise do discurso visual e acrescenta que o estudo das significa??es pode, al?m de observar o campo da an?lise sobre o produzido, contemplar o da recep??o, devido ao car?ter rec?proco do processo de comunica??o. A proposta encontra eco na d?vida comum a muitos receptores, diante de uma imagem: s? eu estou vendo isto? D?vida que acomete tamb?m muitos analistas da imagem e remete ao que existe de mais b?sico em uma imagem: seus aspectos estruturais, evocados pela Teoria. Assim, indica um modelo de aplicabilidade da Teoria Geral da Imagem para o estudo da produ??o de sentidos na recep??o, discutindo a validade da Teoria e considerando que das estruturas se formam os padr?es memorizados e pass?veis de constituir cultura. A pesquisa explorat?ria, a partir do modelo proposto, analisa experi?ncias concretas, com receptores volunt?rios, sobre os efeitos de sentido na interlocu??o entre o produzido e a recep??o de cen?rio do programa de televis?o Conversas Cruzadas, de capas das revistas Vogue e Times e de an?ncios da WWF e Panasonic. Os resultados apontam que o cruzamento de an?lises apresenta contribui??es para o campo de estudos visuais e para o aprimoramento das pr?ticas profissionais na ?rea.