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Journal articles on the topic 'Receptor cross-interference'

Author: Grafiati
Published: 2 June 2025
Last updated: 31 July 2025

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1

Dondi, Elisabetta, Els Pattyn, Georges Lutfalla, et al. "Down-modulation of Type 1 Interferon Responses by Receptor Cross-competition for a Shared Jak Kinase." Journal of Biological Chemistry 276, no. 50 (2001): 47004–12. http://dx.doi.org/10.1074/jbc.m104316200.

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In contrast to the large number of class I and II cytokine receptors, only four Janus kinase (Jak) proteins are expressed in mammalian cells, implying the shared use of these kinases by many different receptor complexes. Consequently, if receptor numbers exceed the amount of available Jak, cross-interference patterns can be expected. We have engineered two model cellular systems expressing two different exogenous Tyk2-interacting receptors. A receptor chimera was generated wherein the extracellular part of the interferon type 1 receptor (Ifnar1) component of the interferon-α/β receptor is repl
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Kutoh, E., P. E. Strömstedt, and L. Poellinger. "Functional interference between the ubiquitous and constitutive octamer transcription factor 1 (OTF-1) and the glucocorticoid receptor by direct protein-protein interaction involving the homeo subdomain of OTF-1." Molecular and Cellular Biology 12, no. 11 (1992): 4960–69. http://dx.doi.org/10.1128/mcb.12.11.4960-4969.1992.

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The ubiquitous and constitutive octamer transcription factor OTF-1 (Oct 1) is the target of positive regulation by the potent herpes simplex virus trans-activator VP16, which forms a complex with the homeodomain of OTF-1. Here we present evidence that the glucocorticoid receptor can negatively regulate OTF-1 function by a mechanism that is independent of DNA binding. In vivo-expressed glucocorticoid receptor inhibited in a hormone-dependent manner activation of a minimal promoter construct carrying a functional octamer site. Moreover, expression of the receptor in vivo resulted in hormone-depe
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3

Kutoh, E., P. E. Strömstedt, and L. Poellinger. "Functional interference between the ubiquitous and constitutive octamer transcription factor 1 (OTF-1) and the glucocorticoid receptor by direct protein-protein interaction involving the homeo subdomain of OTF-1." Molecular and Cellular Biology 12, no. 11 (1992): 4960–69. http://dx.doi.org/10.1128/mcb.12.11.4960.

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The ubiquitous and constitutive octamer transcription factor OTF-1 (Oct 1) is the target of positive regulation by the potent herpes simplex virus trans-activator VP16, which forms a complex with the homeodomain of OTF-1. Here we present evidence that the glucocorticoid receptor can negatively regulate OTF-1 function by a mechanism that is independent of DNA binding. In vivo-expressed glucocorticoid receptor inhibited in a hormone-dependent manner activation of a minimal promoter construct carrying a functional octamer site. Moreover, expression of the receptor in vivo resulted in hormone-depe
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4

De Coster, Sam, and Nicolas van Larebeke. "Endocrine-Disrupting Chemicals: Associated Disorders and Mechanisms of Action." Journal of Environmental and Public Health 2012 (2012): 1–52. http://dx.doi.org/10.1155/2012/713696.

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The incidence and/or prevalence of health problems associated with endocrine-disruption have increased. Many chemicals have endocrine-disrupting properties, including bisphenol A, some organochlorines, polybrominated flame retardants, perfluorinated substances, alkylphenols, phthalates, pesticides, polycyclic aromatic hydrocarbons, alkylphenols, solvents, and some household products including some cleaning products, air fresheners, hair dyes, cosmetics, and sunscreens. Even some metals were shown to have endocrine-disrupting properties. Many observations suggesting that endocrine disruptors do
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5

Vegna, Serena, Damien Gregoire, Marie Moreau, et al. "NOD1 Participates in the Innate Immune Response Triggered by Hepatitis C Virus Polymerase." Journal of Virology 90, no. 13 (2016): 6022–35. http://dx.doi.org/10.1128/jvi.03230-15.

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ABSTRACTHepatitis C virus (HCV) triggers innate immunity signaling in the infected cell. Replication of the viral genome is dispensable for this phenotype, and we along with others have recently shown that NS5B, the viral RNA-dependent RNA polymerase, synthesizes double-stranded RNA (dsRNA) from cellular templates, thus eliciting an inflammatory response, notably via activation of type I interferon and lymphotoxin β. Here, we investigated intracellular signal transduction pathways involved in this process. Using HepaRG cells, a model that largely recapitulates thein vivocomplexities of the inn
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6

Leis, Hugo, Angustias Page, Angel Ramírez, et al. "Glucocorticoid Receptor Counteracts Tumorigenic Activity of Akt in Skin through Interference with the Phosphatidylinositol 3-Kinase Signaling Pathway." Molecular Endocrinology 18, no. 2 (2004): 303–11. http://dx.doi.org/10.1210/me.2003-0350.

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Abstract The skin-targeted overexpression of the glucocorticoid receptor (GR) in transgenic mice dramatically impairs the inflammatory responses to tumor promoter agents and suppresses skin tumor development. The antiinflammatory, rapid effects of corticosteroids are partially exerted through interference of GR with the phosphatidylinositol 3-kinase (PI3K)/Akt signaling pathway in several tissues, a highly relevant pathway in the mouse skin tumor progression process. In this work, we aimed to elucidate whether a cross-talk mechanism between GR and PI3K/Akt occurred in intact skin as well as th
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7

Boleij, Annemarie, Coby M. Laarakkers, Jolein Gloerich, Dorine W. Swinkels, and Harold Tjalsma. "Surface-Affinity Profiling To Identify Host-Pathogen Interactions." Infection and Immunity 79, no. 12 (2011): 4777–83. http://dx.doi.org/10.1128/iai.05572-11.

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ABSTRACTProteolytic treatment of intact bacterial cells has proven to be a convenient approach for the identification of surface-exposed proteins. This class of proteins directly interacts with the outside world, for instance, during adherence to human epithelial cells. Here, we aimed to identify host receptor proteins by introducing a preincubation step in which bacterial cells were first allowed to capture human proteins from epithelial cell lysates. UsingStreptococcus gallolyticusas a model bacterium, liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis of proteolytically rele
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Matthews, Jason, Björn Wihlén, Jane Thomsen та Jan-Åke Gustafsson. "Aryl Hydrocarbon Receptor-Mediated Transcription: Ligand-Dependent Recruitment of Estrogen Receptor α to 2,3,7,8-Tetrachlorodibenzo- p-Dioxin-Responsive Promoters". Molecular and Cellular Biology 25, № 13 (2005): 5317–28. http://dx.doi.org/10.1128/mcb.25.13.5317-5328.2005.

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ABSTRACT Using chromatin immunoprecipitation assays, we studied the 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)-mediated recruitment of the aryl hydrocarbon receptor (AhR) and several coregulators to the CYP1A1 promoter. AhR displayed a time-dependent recruitment, reaching a peak at 75 min and maintaining promoter occupancy for the remainder of the time course. Recruitment of AhR was followed by TIF2/SRC2, which preceded CBP, histone H3 acetylation, and RNA polymerase II (RNAPII). Simultaneous recruitment to the enhancer and the TATA box region suggests the formation of a large multiprotein com
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9

Albee, Lauren J., Heather M. LaPorte, Xianlong Gao, et al. "Identification and functional characterization of arginine vasopressin receptor 1A : atypical chemokine receptor 3 heteromers in vascular smooth muscle." Open Biology 8, no. 1 (2018): 170207. http://dx.doi.org/10.1098/rsob.170207.

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Recent observations suggest that atypical chemokine receptor (ACKR)3 and chemokine (C-X-C motif) receptor (CXCR)4 regulate human vascular smooth muscle function through hetero-oligomerization with α 1 -adrenoceptors. Here, we show that ACKR3 also regulates arginine vasopressin receptor (AVPR)1A. We observed that ACKR3 agonists inhibit arginine vasopressin (aVP)-induced inositol trisphosphate (IP 3 ) production in human vascular smooth muscle cells (hVSMCs) and antagonize aVP-mediated constriction of isolated arteries. Proximity ligation assays, co-immunoprecipitation and bioluminescence resona
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10

Lin, Yuting, John Kokontis, Fangming Tang, et al. "Androgen and Its Receptor Promote Bax-Mediated Apoptosis." Molecular and Cellular Biology 26, no. 5 (2006): 1908–16. http://dx.doi.org/10.1128/mcb.26.5.1908-1916.2006.

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ABSTRACT Androgen and its receptor (AR) have been reported to have pro- or antiapoptotic functions. However, the underlying molecular mechanism is incompletely understood. We report here that androgen and AR promote Bax-mediated apoptosis in prostate cancer cells. UV irradiation and ectopic expression of Bax induce apoptosis in AR-positive, but not AR-negative prostate cancer cells. UV- and Bax-induced apoptosis is abrogated in AR-positive cells that express small interference RNA (siRNA) of AR and is sensitized by reintroduction of AR into AR-negative cells. Although AR is able to promote Bax
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11

Zakaria, Shaheen, Timothy S. Gomez, Doris N. Savoy, et al. "Differential Regulation of TCR-mediated Gene Transcription by Vav Family Members." Journal of Experimental Medicine 199, no. 3 (2004): 429–34. http://dx.doi.org/10.1084/jem.20031228.

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Although all three Vav family members are expressed in T lymphocytes, the role that Vav3 plays in T cell activation is poorly defined. Here we show that, like Vav1, Vav3 undergoes rapid tyrosine phosphorylation after T cell receptor (TCR) cross-linkage and interacts with the adaptor molecules SLP76 and 3BP2 in a SH2-dependent manner. However, depletion of Vav1 but not Vav3 protein by RNA interference affects TCR-mediated IL-2 promoter activity. In contrast, Vav3 function is specifically required for coupling TCR stimulation to serum response element–mediated gene transcription. These data indi
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12

Gehin, Johanna E., Rolf A. Klaasen, Ellen S. Norli, et al. "Rheumatoid factor and falsely elevated results in commercial immunoassays: data from an early arthritis cohort." Rheumatology International 41, no. 9 (2021): 1657–65. http://dx.doi.org/10.1007/s00296-021-04865-9.

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AbstractThe aim of the study was to assess RF cross-reactivity to animal antibodies used in immunoassays, and to test if selected commercial immunoassays are vulnerable to interference from RF, causing false test results. Our study included samples from patients with RF-positive rheumatoid arthritis (RA) and controls (patients with RF-negative RA and psoriatic arthritis), included in an early arthritis-cohort. Reactivity to mouse IgG1, mouse IgG2a, rabbit IgG, bovine IgG, sheep/goat IgG and human IgG was analysed using in-house interference assays. RF-positive sera with strong reactivity to mo
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13

Evaul, Kristen, Michelle Jamnongjit, Bala Bhagavath та Stephen R. Hammes. "Testosterone and Progesterone Rapidly Attenuate Plasma Membrane Gβγ-Mediated Signaling in Xenopus laevis Oocytes by Signaling through Classical Steroid Receptors". Molecular Endocrinology 21, № 1 (2007): 186–96. http://dx.doi.org/10.1210/me.2006-0301.

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Abstract Many transcription-independent (nongenomic) steroid effects are regulated by G proteins. A well-established, biologically relevant example of steroid/G protein interplay is steroid-triggered oocyte maturation, or meiotic resumption, in Xenopus laevis. Oocyte maturation is proposed to occur through a release of inhibition mechanism whereby constitutive signaling by Gβγ and other G proteins maintains oocytes in meiotic arrest. Steroids (androgens in vivo, and androgens and progesterone in vitro) overcome this inhibition to promote meiotic resumption. To test this model, we used G protei
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14

Ghosh, Pradipta, Janice Griffith, Hans J. Geuze, and Stuart Kornfeld. "Mammalian GGAs act together to sort mannose 6-phosphate receptors." Journal of Cell Biology 163, no. 4 (2003): 755–66. http://dx.doi.org/10.1083/jcb.200308038.

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The GGAs (Golgi-localized, γ ear–containing, ADP ribosylation factor–binding proteins) are multidomain proteins implicated in protein trafficking between the Golgi and endosomes. We examined whether the three mammalian GGAs act independently or together to mediate their functions. Using cryo-immunogold electron microscopy, the three GGAs were shown to colocalize within coated buds and vesicles at the trans-Golgi network (TGN) of HeLa cells. In vitro binding experiments revealed multidomain interactions between the GGAs, and chemical cross-linking experiments demonstrated that GGAs 1 and 2 form
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15

Palmieri, Gabriella, Valentino Tullio, Alessandra Zingoni, et al. "CD94/NKG2-A Inhibitory Complex Blocks CD16-Triggered Syk and Extracellular Regulated Kinase Activation, Leading to Cytotoxic Function of Human NK Cells." Journal of Immunology 162, no. 12 (1999): 7181–88. http://dx.doi.org/10.4049/jimmunol.162.12.7181.

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Abstract The CD94/NKG2-A complex is the inhibitory receptor for the nonclassical MHC class I molecule HLA-E on human NK cells. Here we studied the molecular mechanisms underlying the inhibitory activity of CD94/NKG2-A on NK cell functions by analyzing its interference on CD16-initiated signaling pathways involved in the control of cytolytic activity. Both tyrosine phosphorylation and activation of Syk kinase together with tyrosine phosphorylation of CD16 receptor ζ subunit are markedly inhibited by the coengagement of CD94/NKG2-A complex. As a downstream consequence, CD94/NKG2-A cross-linking
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16

Pedrosa, Route, Benjamin Schrijver, Rute B. Marques та ін. "BSCI-25. THE ROLE OF THE IFNγ PATHWAY IN BREAST CANCER BRAIN METASTASIS FORMATION". Neuro-Oncology Advances 1, Supplement_1 (2019): i5. http://dx.doi.org/10.1093/noajnl/vdz014.021.

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Abstract In previous work, we showed the prominence of the T cell response in the formation of brain metastases of primary ER-negative breast cancers. We also showed that prior co-cultured breast cancer cells with stimulated T lymphocytes bear an overexpression of Guanylate-binding protein 1 (GBP1) and possess an increased trespassing ability through an in vitro blood-brain barrier (BBB) model. In addition, we demonstrated a predilection for metastasizing to the brain of breast cancer cells that were co-cultured with activated T cells in a mouse model. In the present work, we show that activat
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17

Alvarez-Suarez, Paloma, Natalia Nowak, Anna Protasiuk-Filipunas, Hiroyuki Yamazaki, Tomasz J. Prószyński, and Marta Gawor. "Drebrin Regulates Acetylcholine Receptor Clustering and Organization of Microtubules at the Postsynaptic Machinery." International Journal of Molecular Sciences 22, no. 17 (2021): 9387. http://dx.doi.org/10.3390/ijms22179387.

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Proper muscle function depends on the neuromuscular junctions (NMJs), which mature postnatally to complex “pretzel-like” structures, allowing for effective synaptic transmission. Postsynaptic acetylcholine receptors (AChRs) at NMJs are anchored in the actin cytoskeleton and clustered by the scaffold protein rapsyn, recruiting various actin-organizing proteins. Mechanisms driving the maturation of the postsynaptic machinery and regulating rapsyn interactions with the cytoskeleton are still poorly understood. Drebrin is an actin and microtubule cross-linker essential for the functioning of the s
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18

Assavalapsakul, Wanchai, Duncan R. Smith, and Sakol Panyim. "Identification and Characterization of a Penaeus monodon Lymphoid Cell-Expressed Receptor for the Yellow Head Virus." Journal of Virology 80, no. 1 (2006): 262–69. http://dx.doi.org/10.1128/jvi.80.1.262-269.2006.

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ABSTRACT The yellow head virus is a positive-sense, single-stranded RNA virus that causes significant mortality in farmed penaeid shrimp. This study sought to isolate and characterize the receptor protein used by the virus to gain entry into Penaeus monodon Oka (lymphoid) organ cells, a primary target of yellow head virus infections. Virus overlay protein binding assay on Oka organ membrane preparations identified a 65-kDa protein, and antibodies raised against this protein inhibited virus entry in primary Oka cell cultures by approximately 80%. N-terminal sequence analysis of the 65-kDa prote
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19

Oh, S. K., and D. P. Lapenson. "E receptor-related immunosuppressive factor in malignant pleural fluid and plasma: molecular mechanism of action on DNA-polymerase-alpha." Journal of Immunology 135, no. 1 (1985): 355–61. http://dx.doi.org/10.4049/jimmunol.135.1.355.

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Abstract We previously purified a potent serum suppressor factor from malignant ascites fluid and showed that it had serologic cross-reactivity with E receptor of human T lymphocytes. We termed this factor "suppressive E receptor factor" (SER). Subsequent studies on SER showed that SER interfered with the production of interleukin 1 and 2 as well as interfering with their activities on target cells. However, SER was not directly cytotoxic to lymphocytes. In this study, we compared the inhibitor of DNA-polymerase (IDP) activity with the suppressive activity on phytohemagglutinin-induced DNA syn
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Manolopoulou, Jenny, Younes Alami, Stephan Petersenn, et al. "Automated 22-kD Growth Hormone–Specific Assay without Interference from Pegvisomant." Clinical Chemistry 58, no. 10 (2012): 1446–56. http://dx.doi.org/10.1373/clinchem.2012.188128.

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Abstract BACKGROUND Large variability exists among different growth hormone (GH) assays owing to differences in calibration, antibody specificity, isoform recognition, and interference from GH binding protein (GHBP). The GH receptor antagonist Pegvisomant presents a new challenge because Pegvisomant interferes with many GH assays. A recent consensus conference established criteria for standardization and evaluation of GH assays. Following consensus recommendations, we developed a new GH assay on an automated analyzer (IDS-iSYS, Immunodiagnostic Systems). METHODS A monoclonal antibody not cross
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21

Harbison, Carole E., Wendy S. Weichert, Brittney L. Gurda, John A. Chiorini, Mavis Agbandje-McKenna, and Colin R. Parrish. "Examining the cross-reactivity and neutralization mechanisms of a panel of mAbs against adeno-associated virus serotypes 1 and 5." Journal of General Virology 93, no. 2 (2012): 347–55. http://dx.doi.org/10.1099/vir.0.035113-0.

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Neutralizing antibodies play a central role in the prevention and clearance of viral infections, but can be detrimental to the use of viral capsids for gene delivery. Antibodies present a major hurdle for ongoing clinical trials using adeno-associated viruses (AAVs); however, relatively little is known about the antigenic epitopes of most AAV serotypes or the mechanism(s) of antibody-mediated neutralization. We developed panels of AAV mAbs by repeatedly immunizing mice with AAV serotype 1 (AAV1) capsids, or by sequentially immunizing with AAV1 followed by AAV5 capsids, in order to examine the
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22

Babcock, Lyle W., Mark Knoblauch, and Mark S. F. Clarke. "The role of myostatin and activin receptor IIB in the regulation of unloading-induced myofiber type-specific skeletal muscle atrophy." Journal of Applied Physiology 119, no. 6 (2015): 633–42. http://dx.doi.org/10.1152/japplphysiol.00762.2014.

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Chronic unloading induces decrements in muscle size and strength. This adaptation is governed by a number of molecular factors including myostatin, a potent negative regulator of muscle mass. Myostatin must first be secreted into the circulation and then bind to the membrane-bound activin receptor IIB (actRIIB) to exert its atrophic action. Therefore, we hypothesized that myofiber type-specific atrophy observed after hindlimb suspension (HLS) would be related to myofiber type-specific expression of myostatin and/or actRIIB. Wistar rats underwent HLS for 10 days, after which the tibialis anteri
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23

Abouzied, Mohamed, Michael Sarzynski, Aaron Walsh, Heather Wood, and Mark Mozola. "Validation Study of a Receptor-Based Lateral Flow Assay for Detection of Beta-Lactam Antibiotics in Milk." Journal of AOAC INTERNATIONAL 92, no. 3 (2009): 959–74. http://dx.doi.org/10.1093/jaoac/92.3.959.

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Abstract Avalidation study designed tomeet the requirements of the AOAC Research Institute and the U.S. Food and Drug Administration (FDA), Center for Veterinary Medicine, was conducted for a receptor-based, immunochromatographic method (BetaStar US) for detection of beta-lactam antibiotic residues in raw, commingled bovine milk. The assay was found to detect amoxicillin, ampicillin, cephapirin, cloxacillin, and penicillin G at levels below the FDA tolerance/safe levels but above the maximum sensitivity thresholds established by the National Conference on Interstate Milk Shipments. Results of
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24

Lee, Sang Woo, Byeong Hee Kim, and Young Ho Seo. "Olfactory system-inspired electronic nose system using numerous low-cost homogenous and hetrogenous sensors." PLOS ONE 18, no. 12 (2023): e0295703. http://dx.doi.org/10.1371/journal.pone.0295703.

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This paper presents an electronic nose system inspired by the biological olfactory system. When comparing the human olfactory system to that of a dog, it’s worth noting that dogs have 30 times more olfactory receptors and three times as many types of olfactory receptors. This implies that the number of olfactory receptors could be a more important parameter for classifying chemical compounds than the number of receptor types. Instead of using expensive precision sensors, the proposed electronic nose system relies on numerous low-cost homogeneous and heterogeneous sensors with poor cross-interf
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25

Trushin, Sergey A., Kevin N. Pennington, Eva M. Carmona та ін. "Protein Kinase Cα (PKCα) Acts Upstream of PKCθ To Activate IκB Kinase and NF-κB in T Lymphocytes". Molecular and Cellular Biology 23, № 19 (2003): 7068–81. http://dx.doi.org/10.1128/mcb.23.19.7068-7081.2003.

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ABSTRACT NF-κB is an ubiquitous transcription factor that is a key in the regulation of the immune response and inflammation. T-cell receptor (TCR) cross-linking leads to NF-κB activation, an IκB kinase (IKK)-dependent process. However, the upstream kinases that regulate IKK activity following TCR activation remain to be fully characterized. Herein, we demonstrate using genetic analysis, pharmacological inhibition, and RNA interference (RNAi) that the conventional protein kinase C (PKC) isoform PKCα, but not PKCβ1, is required for the activation of the IKK complex following T-cell activation t
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26

Liu, Xingguang, Ming Yao, Nan Li, Chunmei Wang, Yuanyuan Zheng, and Xuetao Cao. "CaMKII promotes TLR-triggered proinflammatory cytokine and type I interferon production by directly binding and activating TAK1 and IRF3 in macrophages." Blood 112, no. 13 (2008): 4961–70. http://dx.doi.org/10.1182/blood-2008-03-144022.

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Abstract Calcium and its major downstream effector, calcium/calmodulin-dependent protein kinase II (CaMKII), are found to be important for the functions of immune cells. Lipopolysaccharide (LPS) has been shown to induce intracellular calcium release in macrophages; however, whether and how CaMKII is required for Toll-like receptor (TLR) signaling remain unknown. Here we demonstrate that TLR 4, 9, and 3 ligands markedly induce intracellular calcium fluxes and activate CaMKII-α in macrophages. Selective inhibition or RNA interference of CaMKII significantly suppresses TLR4, 9, 3-triggered produc
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Sanchez-Pacheco, A., T. Palomino, and A. Aranda. "Negative regulation of expression of the pituitary-specific transcription factor GHF-1/Pit-1 by thyroid hormones through interference with promoter enhancer elements." Molecular and Cellular Biology 15, no. 11 (1995): 6322–30. http://dx.doi.org/10.1128/mcb.15.11.6322.

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Expression of the growth hormone gene is due to the presence of the pituitary-specific transcription factor GHF-1/Pit-1. The action of the thyroid hormone T3 is mediated by nuclear receptors that regulate transcription by interaction with DNA elements located near promoters of the regulated genes. In this study, we show that T3 inhibits expression of the GHF-1/Pit-1 gene in rat pituitary GH4C1 cells by a novel mechanism that involves transcriptional interference with other regulatory elements of the promoter. Sequences between bp -90 and -200 of the rat GHF-1/Pit-1 gene which do not contain a
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Georgiou, Marios, and Guy Tear. "Commissureless is required both in commissural neurones and midline cells for axon guidance across the midline." Development 129, no. 12 (2002): 2947–56. http://dx.doi.org/10.1242/dev.129.12.2947.

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In the absence of Commissureless (Comm) function, axons are unable to extend across the central nervous system midline. Comm downregulates levels of Roundabout (Robo), a receptor for the midline repellent Slit, in order to allow axons to cross the midline. comm transcript is expressed at high levels in the midline glia and Comm protein accumulates on axons at the midline. This has led to the hypothesis that Comm moves from the midline glia to the axons, where it can reduce Robo levels. We have found that expression of Comm in the midline cells is unable to rescue the comm phenotype and that ta
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Lupu, Diana, Marcus O. D. Sjödin, Mukesh Varshney, Johan Lindberg, Felicia Loghin, and Joëlle Rüegg. "FLUOXETINE MODULATES SEX STEROID LEVELS IN VITRO." Medicine and Pharmacy Reports 90, no. 4 (2017): 420–24. http://dx.doi.org/10.15386/cjmed-868.

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Background and aims. Selective serotonin reuptake inhibitors (SSRIs) are antidepressants increasingly prescribed against depression during and after pregnancy. However, these compounds cross the placenta and are found in breast milk, thus reaching, and possibly affecting, the fetus and infant during critical developmental stages. Fluoxetine (FLX), a widely used SSRI, can interfere with estrogen signaling, which is important for the development of female sex organs and certain brain areas, among others. Interference with estrogen signaling can take place on different levels, e.g., by affecting
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WIDÉN, Christina, Jan-Åke GUSTAFSSON, and Ann-Charlotte WIKSTRÖM. "Cytosolic glucocorticoid receptor interaction with nuclear factor-kappaB proteins in rat liver cells." Biochemical Journal 373, no. 1 (2003): 211–20. http://dx.doi.org/10.1042/bj20030175.

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The glucocorticoid receptor (GR) acts as an anti-inflammatory factor. To a large extent, this activity is exerted by the interference of pro-inflammatory nuclear factor κB (NF-κB) activity. In their respective inactive forms, both GR and NF-κB reside in the cytoplasm and translocate to the nucleus on relevant stimulation. Previously, p65, a component of the NF-κB complex, and GR have been shown to interact physically in vitro, and the interaction is assumed to take place in the nucleus of cells [McKay and Cidlowski (1999) Endocrine Rev. 20, 435–459]. We have studied the interaction between GR
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Martin, Mickey M., Jessica A. Buckenberger, Jinmai Jiang та ін. "TGF-β1 stimulates human AT1 receptor expression in lung fibroblasts by cross talk between the Smad, p38 MAPK, JNK, and PI3K signaling pathways". American Journal of Physiology-Lung Cellular and Molecular Physiology 293, № 3 (2007): L790—L799. http://dx.doi.org/10.1152/ajplung.00099.2007.

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Both angiotensin II (ANG II) and transforming growth factor-β1 (TGF-β1) are thought to be involved in mediating pulmonary fibrosis. Interactions between the renin-angiotensin system (RAS) and TGF-β1 have been well documented, with most studies describing the effect of ANG II on TGF-β1 expression. However, recent gene expression profiling experiments demonstrated that the angiotensin II type 1 receptor (AT1R) gene was a novel TGF-β1 target in human adult lung fibroblasts. In this report, we show that TGF-β1 augments human AT1R (hAT1R) steady-state mRNA and protein levels in a dose- and time-dep
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Pedrosa, R., J. M. Kros, B. Schrijver та ін. "P11.10 The IFNγ pathway mediates brain metastasis formation of breast cancer". Neuro-Oncology 21, Supplement_3 (2019): iii44. http://dx.doi.org/10.1093/neuonc/noz126.156.

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Abstract BACKGROUND In previous work we showed the prominence of the T-cell response in the formation of brain metastases of primary ER negative breast cancers (Mustafa et al, Acta Neuropathol 2018). We also showed that breast cancer cells co-cultured with stimulated T lymphocytes overexpress Guanylate-binding protein 1 (GBP1) accompanying increased trespassing ability through an in vitro blood-brain barrier (BBB) model. In addition, we demonstrated a predilection for metastasizing to brain of breast cancer cells that were co-cultured with activated T cells in a mouse model. We now scrutinize
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Vashistha, Himanshu, Pravin C. Singhal, Ashwani Malhotra, et al. "Null mutations at the p66 and bradykinin 2 receptor loci induce divergent phenotypes in the diabetic kidney." American Journal of Physiology-Renal Physiology 303, no. 12 (2012): F1629—F1640. http://dx.doi.org/10.1152/ajprenal.00246.2012.

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Candidate genes have been identified that confer increased risk for diabetic glomerulosclerosis (DG). Mice heterozygous for the Akita (Ins2+/C96Y) diabetogenic mutation with a second mutation introduced at the bradykinin 2 receptor (B2R−/−) locus express a disease phenotype that approximates human DG. Src homology 2 domain transforming protein 1 (p66) controls mitochondrial metabolism and cellular responses to oxidative stress, aging, and apoptosis. We generated p66-null Akita mice to test whether inactivating mutations at the p66 locus will rescue kidneys of Akita mice from disease-causing mu
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34

Abouzied, Mohamed, Dana Driksna, Coilin Walsh, et al. "Validation Study of the BetaStar® Plus Lateral Flow Assay for Detection of Beta-Lactam Antibiotics in Milk." Journal of AOAC INTERNATIONAL 95, no. 4 (2012): 1211–21. http://dx.doi.org/10.5740/jaoacint.11-252.

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Abstract A validation study designed to meet the requirements of the AOAC Research Institute and the U.S. Food and Drug Administration, Center for Veterinary Medicine (FDA/CVM) was conducted for a receptor and antibody-based, immunochromatographic method (BetaStar® Plus) for detection of beta-lactam antibiotic residues in raw, commingled bovine milk. The assay was found to detect amoxicillin, ampicillin, ceftiofur, cephapirin, cloxacillin, and penicillin G at levels below the FDA tolerance/safe levels, but above the maximum sensitivity thresholds established by the National Conference on Inter
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PANI, Giovanni, Renata COLAVITTI, Silvia BORRELLO, and Tommaso GALEOTTI. "Endogenous oxygen radicals modulate protein tyrosine phosphorylation and JNK-1 activation in lectin-stimulated thymocytes." Biochemical Journal 347, no. 1 (2000): 173–81. http://dx.doi.org/10.1042/bj3470173.

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Molecular events mediating the T-lymphocyte response to lectins are still incompletely understood, although much evidence suggests that both the mitogenic and the death-promoting effects of these agents involve the biochemical cascade initiated by the CD3/T-cell antigen receptor (TCR) complex. Reactive oxygen species (ROS) and in particular H2O2 have been shown to have a role in cell response to cytokines and growth factors. Here we report that the proliferation of mouse thymocytes in response to the mitogenic lectin concanavalin A (ConA) is strongly and selectively inhibited by the intracellu
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36

Mok, Darren Z. L., and Kuan Rong Chan. "The Effects of Pre-Existing Antibodies on Live-Attenuated Viral Vaccines." Viruses 12, no. 5 (2020): 520. http://dx.doi.org/10.3390/v12050520.

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Live-attenuated vaccines (LAVs) have achieved remarkable successes in controlling virus spread, as well as for other applications such as cancer immunotherapy. However, with rapid increases in international travel, globalization, geographic spread of viral vectors, and widespread use of vaccines, there is an increasing need to consider how pre-exposure to viruses which share similar antigenic regions can impact vaccine efficacy. Pre-existing antibodies, derived from either from maternal–fetal transmission, or by previous infection or vaccination, have been demonstrated to interfere with vaccin
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37

Tiruthani, Karthik, Adam Mischler, Shoeb Ahmed, Jessica Mahinthakumar, Jason M. Haugh, and Balaji M. Rao. "Design and evaluation of engineered protein biosensors for live-cell imaging of EGFR phosphorylation." Science Signaling 12, no. 584 (2019): eaap7584. http://dx.doi.org/10.1126/scisignal.aap7584.

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Live-cell fluorescence microscopy is broadly applied to study the dynamics of receptor-mediated cell signaling, but the availability of intracellular biosensors is limited. A biosensor based on the tandem SH2 domains from phospholipase C–γ1 (PLCγ1), tSH2-WT, has been used to measure phosphorylation of the epidermal growth factor receptor (EGFR). Here, we found that tSH2-WT lacked specificity for phosphorylated EGFR, consistent with the known promiscuity of SH2 domains. Further, EGF-stimulated membrane recruitment of tSH2-WT differed qualitatively from the expected kinetics of EGFR phosphorylat
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38

Gehin, J. E., R. A. Klaasen, E. S. Norli, et al. "FRI0579 RHEUMATOID FACTOR IS ASSOCIATED WITH FALSELY ELEVATED RESULTS IN COMMERCIAL IMMUNOASSAYS: DATA FROM AN EARLY ARTHRITIS COHORT." Annals of the Rheumatic Diseases 79, Suppl 1 (2020): 893. http://dx.doi.org/10.1136/annrheumdis-2020-eular.4144.

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Background:Immunoassays are used to measure a range of analytes in clinical laboratories. Rheumatoid factor (RF) and other patient antibodies, such as heterophilic antibodies, can bind animal antibodies used in immunoassays and cause erroneous results, which may lead to misdiagnosis and incorrect treatment of patients.1Objectives:To assess RF reactivity to animal antibodies and to test if selected commercial immunoassays are vulnerable to interference from RF-positive sera.Methods:Samples from 124 patients with RF-positive rheumatoid arthritis (RA) included in the Norwegian Very Early Arthriti
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Strazza, Marianne, Inbar Azoulay-Alfaguter, Michael Peled та ін. "PLCε1 regulates SDF-1α–induced lymphocyte adhesion and migration to sites of inflammation". Proceedings of the National Academy of Sciences 114, № 10 (2017): 2693–98. http://dx.doi.org/10.1073/pnas.1612900114.

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Regulation of integrins is critical for lymphocyte adhesion to endothelium and migration throughout the body. Inside-out signaling to integrins is mediated by the small GTPase Ras-proximate-1 (Rap1). Using an RNA-mediated interference screen, we identified phospholipase Cε 1 (PLCε1) as a crucial regulator of stromal cell-derived factor 1 alpha (SDF-1α)-induced Rap1 activation. We have shown that SDF-1α-induced activation of Rap1 is transient in comparison with the sustained level following cross-linking of the antigen receptor. We identified that PLCε1 was necessary for SDF-1α-induced adhesion
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Filip, Anca T., Ovidiu Balacescu, Catalin Marian, and Andrei Anghel. "Microbiota Small RNAs in Inflammatory Bowel Disease." Journal of Gastrointestinal and Liver Diseases 25, no. 4 (2016): 509–16. http://dx.doi.org/10.15403/jgld.2014.1121.254.lip.

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MiRNAs are a class of potential gene regulators of critical importance in Inflammatory Bowel Disease (IBD). This review aims to present the connection between gut microbiota, probiotics administration and microRNA (miRNA) expression in IBD. It also brings into question cross-kingdom RNAi (RNA interference). Not only that gut host cells garden the intestinal microbiome via miRNA, but also strong evidence supports the idea that different species of bacteria have an impact on the intestinal immune response by modulating miRNA expression. Cross-kingdom RNAi refers to RNA silencing signals that tra
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41

Virtakoivu, Reetta, Teijo Pellinen, Juha K. Rantala, Merja Perälä та Johanna Ivaska. "Distinct roles of AKT isoforms in regulating β1-integrin activity, migration, and invasion in prostate cancer". Molecular Biology of the Cell 23, № 17 (2012): 3357–69. http://dx.doi.org/10.1091/mbc.e12-03-0213.

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AKT1 and AKT2 kinases have been shown to play opposite roles in breast cancer migration and invasion. In this study, an RNA interference screen for integrin activity inhibitors identified AKT1 as an inhibitor of β1-integrin activity in prostate cancer. Validation experiments investigating all three AKT isoforms demonstrated that, unlike in breast cancer, both AKT1 and AKT2 function as negative regulators of cell migration and invasion in PC3 prostate cancer cells. Down-regulation of AKT1 and AKT2, but not AKT3, induced activation of cell surface β1-integrins and enhanced adhesion, migration, a
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42

Nomura, Ryuji, Asuka Kiyota, Etsuko Suzaki, et al. "Human Coronavirus 229E Binds to CD13 in Rafts and Enters the Cell through Caveolae." Journal of Virology 78, no. 16 (2004): 8701–8. http://dx.doi.org/10.1128/jvi.78.16.8701-8708.2004.

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ABSTRACT CD13, a receptor for human coronavirus 229E (HCoV-229E), was identified as a major component of the Triton X-100-resistant membrane microdomain in human fibroblasts. The incubation of living fibroblasts with an anti-CD13 antibody on ice gave punctate labeling that was evenly distributed on the cell surface, but raising the temperature to 37°C before fixation caused aggregation of the labeling. The aggregated labeling of CD13 colocalized with caveolin-1 in most cells. The HCoV-229E virus particle showed a binding and redistribution pattern that was similar to that caused by the anti-CD
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43

Zhao, Mengmeng, Ning Ding, Haoyu Wang та ін. "Activation of TRPA1 in Bladder Suburothelial Myofibroblasts Counteracts TGF-β1-Induced Fibrotic Changes". International Journal of Molecular Sciences 24, № 11 (2023): 9501. http://dx.doi.org/10.3390/ijms24119501.

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The activation of the transient receptor potential ankyrin 1 (TRPA1) channel has anti-fibrotic effects in the lung and intestine. Suburothelial myofibroblasts (subu−MyoFBs), a specialized subset of fibroblasts in the bladder, are known to express TRPA1. However, the role of the TRPA1 in the development of bladder fibrosis remains elusive. In this study, we use the transforming growth factor-β1 (TGF-β1) to induce fibrotic changes in subu−MyoFBs and assess the consequences of TRPA1 activation utilizing RT-qPCR, western blotting, and immunocytochemistry. TGF-β1 stimulation increased α-SMA, collag
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44

Tawfi, Daniel S., Wendy S. Hamilton, Katelyn R. Cowan, and Frederick D. Goldman. "Exogenous Immunoglobulin Downregulates T Cell Receptor Signaling and Cytokine Production." Blood 112, no. 11 (2008): 2559. http://dx.doi.org/10.1182/blood.v112.11.2559.2559.

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Abstract BACKGROUND: Immune globulin, a polyvalent solution of pooled human serum immunoglobulin, when administered intravenously (IGIV) is used as replacement therapy in patients with hypogammaglobulinemia. In addition, IGIV has immunosuppressive properties and is FDA-approved to treat idiopathic thrombocytopenic purpura; it has also been used to treat several other autoimmune diseases including autoimmune hemolytic anemia and Kawasaki Disease. The mechanisms of action underlying IGIVs immunomodulatory effects are poorly understood, although a few reports have shown IGIV can inhibit T cell ac
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45

Kydd, Janel, Rahul Jadia, and Prakash Rai. "Co-Administered Polymeric Nano-Antidotes for Improved Photo-Triggered Response in Glioblastoma." Pharmaceutics 10, no. 4 (2018): 226. http://dx.doi.org/10.3390/pharmaceutics10040226.

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Polymer-based nanoparticles (NPs) are useful vehicles in treating glioblastoma because of their favorable characteristics such as small size and ability to cross the blood–brain barrier, as well as reduced immunogenicity and side effects. The use of a photosensitizer drug such as Verteporfin (BPD), in combination with a pan-vascular endothelial growth factor receptor (VEGFR) tyrosine kinase inhibitor (TKI), Cediranib (CED), encapsulated in NPs will provide the medical field with new research on the possible ways to treat glioblastoma. Concomitant administration of BPD and CED NPs have the pote
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46

Novaković, Jasmina. "Validation of a High-Performance Thin-Layer Chromatographic Method for Trace Analysis for Some Generic Drugs Affecting Gastrointestinal Function." Journal of AOAC INTERNATIONAL 83, no. 6 (2000): 1507–16. http://dx.doi.org/10.1093/jaoac/83.6.1507.

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Abstract To prevent cross-contamination between pharmaceutical products manufactured with the same equipment, cleanup procedures must be introduced before the manufacture of a new product begins. From an analytical point of view, it is crucial to select and validate a suitable analytical method to determine contaminants in the rinse water, swabs, and the placebo of the next product. High performance thin-layer chromatography (HPTLC) was chosen in our laboratory for this purpose and was optimized to meet the requirements of trace determination. The method was validated in terms of the limit of
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47

Chew, C. S., M. Ljungstrom, A. Smolka, and M. R. Brown. "Primary culture of secretagogue-responsive parietal cells from rabbit gastric mucosa." American Journal of Physiology-Gastrointestinal and Liver Physiology 256, no. 1 (1989): G254—G263. http://dx.doi.org/10.1152/ajpgi.1989.256.1.g254.

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A new procedure for isolation and primary culture of gastric parietal cells is described. Parietal cells from rabbit gastric mucosa are enriched to greater than 95% purity by combining a Nycodenz gradient separation with centrifugal elutriation. Cells are plated on the basement membrane matrix, Matrigel, and maintained in culture for at least 1 wk. Parietal cells cultured in this manner remain differentiated, cross-react with monoclonal H+-K+-ATPase antibodies, and respond to histamine, gastrin, and cholinergic stimulation with increased acid production as measured by accumulation of the weak
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48

Liang, Ning, Yinglei Xu, Yimeng Yin та ін. "Steroidogenic Factor-1 Is Required for TGF-β3-Mediated 17β-Estradiol Synthesis in Mouse Ovarian Granulosa Cells". Endocrinology 152, № 8 (2011): 3213–25. http://dx.doi.org/10.1210/en.2011-0102.

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The TGF-β superfamily members are indicated to play key roles in ovarian follicular development, such as granulosa cell proliferation, estrogens, and progesterone production. However, little is known about the roles of TGF-β3 in follicular development. In this study, we found that TGF-β3 was predominantly expressed in granulosa cells of mouse ovarian follicles, and it significantly promoted 17β-estradiol (E2) release in a dose-dependent manner. The orphan nuclear receptor steroidogenic factor-1 (SF-1) was required in TGF-β3-induced Cyp19a1 (a key rate-limiting enzyme for estrogen biosynthesis)
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49

Liljeqvist, Jan-Åke, Karin Önnheim, Petra Tunbäck, et al. "Human Antibodies against Herpes Simplex Virus 2 Glycoprotein G Do Not Neutralize but Mediate Antibody-Dependent Cellular Cytotoxicity." Antibodies 13, no. 2 (2024): 40. http://dx.doi.org/10.3390/antib13020040.

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Herpes simplex virus 2 (HSV-2) is a sexually transmitted infection affecting 491 million individuals globally. Consequently, there is a great need for both prophylactic and therapeutic vaccines. Unfortunately, several vaccine clinical trials, primarily employing the glycoprotein D of HSV-2 (gD-2), have failed. The immune protection conferred by human anti-HSV-2 antibodies in genital infection and disease remains elusive. It is well-known that gD-2 elicits cross-reactive neutralizing antibodies, i.e., anti-gD-2 antibodies recognize gD in HSV-1 (gD-1). In contrast, anti-glycoprotein G in HSV-2 (
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50

Shrestha, Bishow, Swarup Sharma Rijal, Chheki Sherpa, and James Leu. "ODP442 Posaconazole-induced Pseudohyperaldosteronism (PIPH) in Patient Treated for Acute Myeloid Leukemia." Journal of the Endocrine Society 6, Supplement_1 (2022): A720—A721. http://dx.doi.org/10.1210/jendso/bvac150.1484.

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Abstract Case summary A 62-year-old Caucasian female presented to our institute with mechanical fall complicated by cervical spine fracture. She had known history of essential hypertension and recently diagnosed acute myeloid leukemia with myelodysplasia related changes (AML-MRC). She had undergone induction chemotherapy with combination of daunorubicin and cytarabine, resulting in chemotherapy induced prolonged pancytopenia. She was initially started on primary prophylaxis with fluconazole, but was switched to voriconazole due to persistent neutropenia. Patient then developed bilateral upper
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