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Journal articles on the topic 'Rechromatography'

Author: Grafiati
Published: 5 June 2025
Last updated: 1 August 2025

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1

Horner, A. A. "Heterogeneous distribution of antithrombin-binding sites in rat brain heparan sulphate proteoglycans." Biochemical Journal 280, no. 2 (1991): 393–97. http://dx.doi.org/10.1042/bj2800393.

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Heparan sulphates with high binding affinity for antithrombin (HA-HS), labelled in vivo with [35S]sulphate, were extracted from rat brains and purified by chromatography on DEAE-cellulose and on antithrombin-agarose. HA-HS proteoglycans (HA-HSPG) were then separated from HA-HS chains on Sepharose CL-6B. The total HA-HSPG product was rechromatographed on antithrombin-agarose. Six HA-HSPG subfractions with differing degrees of affinity for antithrombin were recovered and treated with NaOH to release their chains. Rechromatography of these six 35S-labelled HS chain preparations on antithrombin-ag
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2

Kotani, T., K. Umeki, S. Yagihashi, K. Hirai, and S. Ohtaki. "Identification of thyroiditogenic epitope on porcine thyroid peroxidase for C57BL/6 mice." Journal of Immunology 148, no. 7 (1992): 2084–89. http://dx.doi.org/10.4049/jimmunol.148.7.2084.

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Abstract C57BL/6 mice show thyroid lesions when immunized with porcine thyroid peroxidase (pTPO) emulsified in CFA. We attempted to clarify a thyroiditogenic epitope on pTPO. Thyroid peroxidase treated with cyanogen bromide was fractionated by reverse phase chromatography, and six fractions (A to F) were obtained. Two of these fractions (D and E) stimulated lymph node cells (LNC) primed with pTPO in vitro and induced thyroiditis in vivo. Tricine-SDS-PAGE and rechromatography showed that fraction D consisted solely of a fragment of Mr 9500 Da and that fraction E contained mainly fragments of Mr
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3

Peterson, Robert E., Gail M. Shannon, and Odette L. Shotwell. "Purification of Cyclopiazonic Acid by Liquid Chromatography." Journal of AOAC INTERNATIONAL 72, no. 2 (1989): 332–35. http://dx.doi.org/10.1093/jaoac/72.2.332.

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Abstract A purification procedure for cyclopiazonic acid has been developed, using sequential preparative and semi-preparative liquid chromatography. Crude cyclopiazonic acid (324 mg) was extracted from a 1 L fermentation medium with chloroform-methanol (80 + 20), dried, dissolved in chloroform, and chromatographed on an oxalic acid/ silica preparative column with chloroform-methanol (99 + 1) as the eluant. A semi-preparative oxalic acid/silica column and chloroform- methanol (99.5 + 0.5) were then used for rechromatography of the partially purified cyclopiazonic acid. This second chromatograp
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4

Bijl, Jan P., Carlos H. Van Peteghem, and Diana A. Dekeyser. "Fluorimetric Determination of Anatoxin M1 in Cheese." Journal of AOAC INTERNATIONAL 70, no. 3 (1987): 472–75. http://dx.doi.org/10.1093/jaoac/70.3.472.

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Abstract A simple and sensitive method is proposed for the determination of aflatoxin M, in cheese. The ground cheese sample is extracted with acetone-water (3 + 1). Acetone is evaporated under vacuum, and the aqueous phase is passed through a C18 disposable cartridge. After the cartridge is washed with acetonitrile-water (1 + 9), the toxin is eluted with acetonitrile. The extract is then cleaned up on a silica cartridge. Final analysis is performed by 2-dimensional thin layer chromatography (TLC) combined with fluorodensitometry or by liquid chromatography on a reverse phase C„ column with fl
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5

Potter, K. A., and R. W. Leid. "Isolation and partial characterization of an eosinophil chemotactic factor from metacestodes of Taenia taeniaeformis (ECF-Tt)." Journal of Immunology 136, no. 5 (1986): 1712–17. http://dx.doi.org/10.4049/jimmunol.136.5.1712.

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Abstract Eosinophil chemotactic activity associated with protein extracts of Taenia taeniaeformis metacestodes was investigated. Chemotactic activity was associated with the nonbound protein after QAE cellulose chromatography of a 3 M KCl extract of homogenized larvae. When this material was precipitated with ammonium sulfate, activity was present in the 40 to 80% precipitate. Upon rechromatography on QAE cellulose equilibrated in a low ionic strength buffer, eosinophil chemotactic activity was retained by the gel and eluted after application of the NaCl gradient. Gel filtration of Sephacryl S
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6

Deagen, J. T., and P. D. Whanger. "Properties of cadmium-binding proteins in rat testes. Characteristics unlike metallothionein." Biochemical Journal 231, no. 2 (1985): 279–83. http://dx.doi.org/10.1042/bj2310279.

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Since the exposure of rats to cadmium causes zinc to accumulate in metallothionein in liver and kidney but not in a similar protein in the testes, the properties of the low-Mr cadmium-binding proteins were investigated in rat testes. Weanling rats that had been given dietary cadmium for 6 weeks were injected with 109CdCl2 and subsequently killed, and the 109Cd-labelled low-Mr proteins from testes were purified. The pooled low-Mr cadmium-containing fractions from the gel-filtration (Sephadex G-75) columns were eluted through DEAE-Sephacel columns, yielding two peaks. Each of the individual peak
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7

Alichanidis, Efstathios, and Alexandra-Maria Michaelidou. "Glycoproteins in the heat- and acid-stable fraction of ovine milk." Journal of Dairy Research 57, no. 4 (1990): 507–15. http://dx.doi.org/10.1017/s0022029900029551.

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SummaryAffinity chromatography on a concanavalin A–Sepharose support was used to isolate two glycoprotein fractions from a heat- and acid-stable fraction of ovine milk. One of these glycoprotein fractions was purified by rechromatography on DEAE-cellulose to essentially a pure protein yielding a single band on gel electro-phoresis. The apparent Mr of this glycoprotein (GP2) as estimated by electrophoresis was 50500. It contained 8·88% carbohydrate and 0·61% P. The other glycoprotein fraction (GP3) contained 0·53% P and 17·76% carbohydrate including sialic acid, mannose, galactose, fucose, gala
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8

Johnson, D. L., and S. L. Wilson. "Identification of a 150-kilodalton polypeptide that copurifies with yeast TFIIIC and binds specifically to tRNA genes." Molecular and Cellular Biology 9, no. 5 (1989): 2018–24. http://dx.doi.org/10.1128/mcb.9.5.2018-2024.1989.

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The transcription in vitro of eucaryotic tRNA genes by RNA polymerase III requires two transcription factors, designated TFIIIB and TFIIIC. One of the critical functions of TFIIIC in the transcription of tRNA genes is that it interacts directly and specifically with the two internal promoter elements of these genes. We have partially purified Saccharomyces cerevisiae TFIIIC by chromatography on Bio-Rex 70, DEAE-cellulose, and phosphocellulose resins. A 150-kilodalton (kDa) DNA-binding polypeptide copurified with TFIIIC activity. This 150-kDa protein coeluted with the DNA-binding activity of TF
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9

Johnson, D. L., and S. L. Wilson. "Identification of a 150-kilodalton polypeptide that copurifies with yeast TFIIIC and binds specifically to tRNA genes." Molecular and Cellular Biology 9, no. 5 (1989): 2018–24. http://dx.doi.org/10.1128/mcb.9.5.2018.

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The transcription in vitro of eucaryotic tRNA genes by RNA polymerase III requires two transcription factors, designated TFIIIB and TFIIIC. One of the critical functions of TFIIIC in the transcription of tRNA genes is that it interacts directly and specifically with the two internal promoter elements of these genes. We have partially purified Saccharomyces cerevisiae TFIIIC by chromatography on Bio-Rex 70, DEAE-cellulose, and phosphocellulose resins. A 150-kilodalton (kDa) DNA-binding polypeptide copurified with TFIIIC activity. This 150-kDa protein coeluted with the DNA-binding activity of TF
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10

Jalmakhanbetova, R. I., Ye M. Suleimen, N. Abe, et al. "Isolation and in silico SARS-CoV-2 main protease inhibition potential of chrysoeriol from Chondrilla brevirostris Fisch. & C.A. Mey." Bulletin of the Karaganda University. "Chemistry" series 105, no. 1 (2022): 78–85. http://dx.doi.org/10.31489/2022ch1/78-85.

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The genus Chondrilla L. comprises 22 species on the CIS territory. 16 species of them grow in Kazakhstan. All species of the genus Chondrilla L. are rubber-bearing herbaceous plants that belong to the Asteraceae family. We picked Chondrilla brevirostris Fisch. & C.A. Mey. for the chemical study. It is a perennial herb that grows in desert steppes and forest meadows. The aboveground parts of Ch. brevirostris were extracted with ethanol at room temperature. Several fractions were obtained by separating ethanol extract on column chromatography. Rechromatography and preparative thin-layer chro
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11

Le, Violeta, Andrey Sukhikh, Timothy Larichev, Svetlana Ivanova, Alexander Prosekov, and Anastasia Dmitrieva. "Isolation of the Main Biologically Active Substances and Phytochemical Analysis of Ginkgo biloba Callus Culture Extracts." Molecules 28, no. 4 (2023): 1560. http://dx.doi.org/10.3390/molecules28041560.

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The work reveals the results of studying the content of biologically active substances in samples of extracts of Ginkgo biloba callus cultures. Callus cultures grown in vitro on liquid nutrient media were the objects of the study. Considering various factors affecting the yield of the target components during extraction, the volume fraction of the organic modifier in the extracting mixture, the temperature factor, and the exposure time were identified as the main ones. The maximum yield of extractive substances (target biologically active substances with a degree of extraction of at least 50%)
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12

Gudzenko, O. V., and L. D. Varbanets. "Isolation and characterization of Bacillus sp. IMV B-7883 proteases." Ukrainian Biochemical Journal 95, no. 5 (2023): 98–107. http://dx.doi.org/10.15407/ubj95.05.098.

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The representatives of Bacillus are some of the best protease producers studied so far since they exhibit broad substrate specificity, significant activity, stability, simple downstream purification, short period of fermentation and low cost. Earlier, we showed that Bacillus sp. IMV B-7883 strain synthesizes an extracellular proteases, which exhibit elastolytic and fibrinogenolytic activity. The aim of the work was to isolate and purify these enzymes from the culture liquid of the Bacillus sp. IMV B-7883 strain, as well as to study their properties. Isolation and purification of proteases was
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13

Berry, Leslie R., Frank V. Puzzuoli, and Mark W. C. Hatton. "On the interaction between 5-hydroxytryptamine and N-acetylneuraminic acid under aqueous conditions." Canadian Journal of Biochemistry and Cell Biology 63, no. 7 (1985): 757–63. http://dx.doi.org/10.1139/o85-095.

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A complex designated 5-HT–NeuAc was formed between 5-hydroxytryptamine (5-HT) and N-acetylneuraminic acid (NeuAc) under aqueous conditions. Complex formation was encouraged by exposure to light (3000–3800 Å; 1 Å = 0.1 nm) and freeze-drying and the freeze-dried complex was isolated by gel filtration chromatography. Although stable to rechromatography on Bio-Gel P-2 if H2O was the eluent, 5-HT–NeuAc dissociated into the free components when placed in 0.1 M NaCl. Chemical analyses of the isolated complex showed that an equimolar amount of 5-HT and NeuAc was present and that all group functions we
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14

Fluge, OSystein, Knut Sletten, Gjermund Fluge, Lage Aksnes, and Said Elsayed. "In Vitro Toxicity of Purified Gluten Peptides Tested by Organ Culture." Journal of Pediatric Gastroenterology and Nutrition 18, no. 2 (1994): 186–92. http://dx.doi.org/10.1002/j.1536-4801.1994.tb11151.x.

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SummaryVarious subfractions of Frazer fraction III were separated by high‐pressure liquid chromatography, and their toxicity in vitro (organ culture) was tested in comparison with α‐gliadin using duodenal biopsies from 25 patients with active celiac disease and subtotal villous atrophy, 2 patients with partial villous atrophy, and 10 nonceliac controls. One dominating fraction, designated Frazer III‐2‐VIH, was purified by several steps of rechromatography. It was markedly toxic to duodenal explants from patients with active celiac disease. The mean enterocyte height after culture was 15.9 μm c
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15

Matsushita, M., and T. Fujita. "Activation of the classical complement pathway by mannose-binding protein in association with a novel C1s-like serine protease." Journal of Experimental Medicine 176, no. 6 (1992): 1497–502. http://dx.doi.org/10.1084/jem.176.6.1497.

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Serum mannose-binding protein (MBP) is a C-type lectin that binds to terminal mannose and N-acetylglucosamine moieties present on surfaces of certain pathogens and activates the classical complement pathway. In the present study, we describe the mechanism underlying the activation triggered by MBP. The human serum MBP fraction was obtained by sequential affinity chromatography on mannan-Sepharose, anti-IgM-Sepharose and anti-MBP-Sepharose in the presence of calcium ions. This fraction contained a C1s-like serine protease as assessed by C4 consumption. The C1s-like serine protease, designated M
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16

Okayama, M., K. Oguri, Y. Fujiwara, et al. "Purification and characterization of human platelet proteoglycan." Biochemical Journal 233, no. 1 (1986): 73–81. http://dx.doi.org/10.1042/bj2330073.

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Freshly prepared platelets were shown to contain glycosaminoglycans equivalent to 530 micrograms of hexuronate/10(11) platelets. When the platelets were extracted with 4 M-guanidinium chloride containing proteinase inhibitors, and the extract was dialysed extensively against 7 M-urea solution, almost all of proteoglycan was recovered in the urea-soluble fraction. The proteoglycan was purified from the urea-soluble fraction with a yield of 47% by DEAE-Sephacel chromatography, CsCl-density-gradient centrifugation, Bio-Gel A-15m gel filtration and then rechromatography on DEAE-Sephacel. The purif
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17

Gänzle, Michael G., Alexandra Höltzel, Jens Walter, Günther Jung, and Walter P. Hammes. "Characterization of Reutericyclin Produced by Lactobacillus reuteri LTH2584." Applied and Environmental Microbiology 66, no. 10 (2000): 4325–33. http://dx.doi.org/10.1128/aem.66.10.4325-4333.2000.

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ABSTRACT Lactobacillus reuteri LTH2584 exhibits antimicrobial activity that can be attributed neither to bacteriocins nor to the production of reuterin or organic acids. We have purified the active compound, named reutericyclin, to homogeneity and characterized its antimicrobial activity. Reutericyclin exhibited a broad inhibitory spectrum including Lactobacillus spp., Bacillus subtilis, B. cereus, Enterococcus faecalis, Staphylococcus aureus, and Listeria innocua. It did not affect the growth of gram-negative bacteria; however, the growth of lipopolysaccharide mutant strains ofEscherichia col
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18

Grotjohann, N. "Regulation of Fructose 1,6-Bisphosphatase Activity of Chlorella by Mole Mass Change." Zeitschrift für Naturforschung C 51, no. 9-10 (1996): 639–45. http://dx.doi.org/10.1515/znc-1996-9-1007.

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Fast protein liquid chromatography on Superose 6 of partially purified FBPase II from Chlorella reveals a 1350 kDa-form at pH 6.0 and a 67 kDa-form at pH 8.5. Treatment of the large enzyme form with 5mᴍ concentrations of Mg2+, F1,6P2, DTT or ATP leads to dissociation into smaller ones of 215 -470 kDa. Aggregation/dissoziation is a reversible process, as has been shown for the effect of F1,6P2 and of pH, by rechromatography. The change in mole mass results in alterations of the activitiy and of the kinetic properties of the enzyme forms, obtained. Dissociation results in a 4 - 6 fold increase i
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19

Grainger, J. L., and M. M. Winkler. "Fertilization triggers unmasking of maternal mRNAs in sea urchin eggs." Molecular and Cellular Biology 7, no. 11 (1987): 3947–54. http://dx.doi.org/10.1128/mcb.7.11.3947-3954.1987.

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Fertilization of sea urchin eggs results in a large increase in the rate of protein synthesis which is mediated by the translation of stored maternal mRNA. The masked message hypothesis suggests that messenger ribonucleoprotein particles (mRNPs) from unfertilized eggs are translationally inactive and that fertilization results in alterations of the mRNPs such that they become translationally active. Previous workers have isolated egg mRNPs by sucrose gradient centrifugation and have assayed their translational activity in heterologous cell-free systems. The conflicting results they obtained ar
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20

Grainger, J. L., and M. M. Winkler. "Fertilization triggers unmasking of maternal mRNAs in sea urchin eggs." Molecular and Cellular Biology 7, no. 11 (1987): 3947–54. http://dx.doi.org/10.1128/mcb.7.11.3947.

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Fertilization of sea urchin eggs results in a large increase in the rate of protein synthesis which is mediated by the translation of stored maternal mRNA. The masked message hypothesis suggests that messenger ribonucleoprotein particles (mRNPs) from unfertilized eggs are translationally inactive and that fertilization results in alterations of the mRNPs such that they become translationally active. Previous workers have isolated egg mRNPs by sucrose gradient centrifugation and have assayed their translational activity in heterologous cell-free systems. The conflicting results they obtained ar
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21

Landt, Michael. "Leptin Binding and Binding Capacity in Serum." Clinical Chemistry 46, no. 3 (2000): 379–84. http://dx.doi.org/10.1093/clinchem/46.3.379.

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Abstract Background: Leptin, a hormone produced primarily by adipose tissue, is known to be present in serum as both monomeric (free) and higher molecular mass (bound) forms, but little is known about the nature of the bound forms or physiological variation in binding capacity. Methods: A new method to quantify the free and bound forms was developed, based on HPLC separation and RIA quantification in chromatography fractions. Reanalysis of specimens after addition of exogenous leptin allowed direct determination of leptin-binding capacity and the degree of saturation of leptin-binding capacity
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22

Brouwer, M., J. Enghild, T. Hoexum-Brouwer, I. Thogersen, and A. Truncali. "Primary structure and tissue-specific expression of blue crab (Callinectes sapidus) metallothionein isoforms." Biochemical Journal 311, no. 2 (1995): 617–22. http://dx.doi.org/10.1042/bj3110617.

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In aquatic animals, synthesis of the metal-binding protein metallothionein (MT) can be induced through exposure to elevated levels of metals in food or water. Whether the different routes of exposure lead to expression of different metallothionein isoforms in different tissues in unknown. In this study we examined the induction of metallothionein isoforms in the hepatopancreas and gills of the blue crab Callinectes sapidus. When blue crabs are exposed to cadmium in their diet, the metal accumulates in the hepatopancreas. Size-exclusion and anion-exchange chromatography show the presence of fiv
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23

Potier, M., L. Michaud, J. Tranchemontagne та L. Thauvette. "Structure of the lysosomal neuraminidase–β-galactosidase–carboxypeptidase multienzymic complex". Biochemical Journal 267, № 1 (1990): 197–202. http://dx.doi.org/10.1042/bj2670197.

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Lysosomal neuraminidase (sialidase; EC 3.2.1.18) and beta-galactosidase (EC 3.2.1.23), together with a carboxypeptidase, the so-called ‘protective protein’, were co-purified from the human placenta by affinity chromatography on a concanavalin A-Sepharose column followed by a thiogalactoside-agarose affinity column for beta-galactosidase. Analysis of the purified material by gel-filtration h.p.l.c. revealed three distinct molecular forms, all with high beta-galactosidase specific activity, but only the largest one expressed neuraminidase activity. Rechromatography of each individual species sep
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24

Oda-Tamai, S., S. Kato, and N. Akamatsu. "Postnatal changes in sialylation of glycoproteins in rat liver." Biochemical Journal 280, no. 1 (1991): 179–85. http://dx.doi.org/10.1042/bj2800179.

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Glycoproteins containing N-linked oligosaccharides were prepared from plasma and liver microsomes of rats aged 0-5 weeks, and galactose and sialic acid content were determined. The sialic acid/galactose ratios in plasma membrane N-glycans remained at about 1 throughout the postnatal period, suggesting that most of the galactose residues are sialylated. In the same way, it was suggested that most of the galactose residues of microsomal N-glycans were sialylated at 0, 4 and 5 weeks of age, but that the degree of sialylation was lower at the other ages, with a minimum at 2 weeks. When the activit
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25

Sepe, S. M., and R. A. Clark. "Oxidant membrane injury by the neutrophil myeloperoxidase system. I. Characterization of a liposome model and injury by myeloperoxidase, hydrogen peroxide, and halides." Journal of Immunology 134, no. 3 (1985): 1888–95. http://dx.doi.org/10.4049/jimmunol.134.3.1888.

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Abstract Neutrophils and other phagocytes can injure cells by means of oxygen-dependent mechanisms, particularly the myeloperoxidase (MPO)-H2O2-halide system. The extent of such damage depends in part on the antioxidant defenses of the target cell. To facilitate the study of this phenomenon, we developed a model system in which we employed liposomes as targets for the myeloperoxidase system. The most useful species of liposomes employed 51Cr as the aqueous space marker and phosphatidyl choline with or without dicetyl phosphate and cholesterol as the structural lipid. Marker entrapment was esta
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26

Wicha-Komsta, Katarzyna, Robert Skibiński, Tomasz Kocki, Waldemar A. Turski, and Łukasz Komsta. "HPLC Gradient Retention of Tryptophan and its Metabolites on Three Stationary Phases in Context of Lipophilicity Assessment." Journal of Chromatographic Science 59, no. 1 (2020): 40–46. http://dx.doi.org/10.1093/chromsci/bmaa074.

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Abstract This paper is a continuation of lipophilicity research on 14 compounds (tryptophan, kynurenine pathway products, auxin pathway products, serotonin pathway products, tryptamine, as well as two synthetic auxin analogs): indole-2-acetic acid sodium salt (IAA), serotonin, 5-hydroxy-L-tryptophan, tryptamine, L-tryptophan, L-kynurenine (KYN), kynurenic acid (KYA), 3-hydroxy-DL-kynurenine, naphtyl-1-acetamide, indole-3-propionic acid (IPA), naphthalene-1-acetic acid (NAA), indole-3-butyric acid (IBA), indole-3-pyruvic acid (IPV), as well as melatonin. They were chromatographed in high perfor
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27

Moats, William A., and Rainer Malisch. "Determination of Cloxacillin and Penicillin V in Milk Using an Automated Liquid Chromatography Cleanup." Journal of AOAC INTERNATIONAL 75, no. 2 (1992): 257–60. http://dx.doi.org/10.1093/jaoac/75.2.257.

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Abstract A number of screening tests will detect β-lactam antibiotics at levels of less than 10 ng/mL in milk. However, confirmatory procedures of comparable sensitivity are not available. A method using an automated liquid chromatography (LC) cleanup sensitive to 1 ng/mL was developed for penicillin V and cloxacillin. Milk was deproteinlzed with 2 volumes of acetonitrlle. Methylene chloride and hexane were added to the filtrate to separate the water layer containing the antibiotics. The filtrate could also be evaporated directly. The water layer was concentrated and loaded onto a polymeric LC
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28

Moats, William A., та Raida Harik-Khan. "Liquid Chromatographic Determination of β-Lactam Antibiotics in Milk: A Multiresidue Approach". Journal of AOAC INTERNATIONAL 78, № 1 (1995): 49–54. http://dx.doi.org/10.1093/jaoac/78.1.49.

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Abstract In the United States, testing of all milk for residues of β-lactam antibiotics is now mandatory. Although a number of screening tests for determination of β-lactam antibiotic residues have been proposed, few reference methods of the required sensitivity (<10 ppb) are available. Methods for determination of several β-lactam antibiotics using an automated liquid chromatography (LC) cleanup have been described recently. This paper describes the integration of these methods into a single extraction and cleanup procedure. Milk was deproteinized with 0.2M Et4NCI and acetonitrile. The
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29

Marenah, L., P. R. Flatt, D. F. Orr, C. Shaw, and Y. H. A. Abdel-Wahab. "Skin secretions of Rana saharica frogs reveal antimicrobial peptides esculentins-1 and -1B and brevinins-1E and -2EC with novel insulin releasing activity." Journal of Endocrinology 188, no. 1 (2006): 1–9. http://dx.doi.org/10.1677/joe.1.06293.

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Skin secretions of Rana saharica were evaluated for the isolation and characterisation of novel insulinotropic peptides. Crude secretions obtained from young adult frogs by mild electrical stimulation of the dorsal skin surface were purified by reverse phase HPLC yielding 80 fractions. In acute 20-min incubations with glucose responsive BRIN-BD11 cells, fractions 36–43, 46–54 and 57–63 significantly stimulated insulin release by 2- to 8-fold compared with 5.6 mM glucose alone. Pooled fractions in the latter two bands were rechromatographed to reveal 9 homogenous peaks, which elicited significa
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30

Manganaro, F., and A. Kuksis. "Rapid isolation of a triacylglycerol synthetase complex from rat intestinal mucosa." Canadian Journal of Biochemistry and Cell Biology 63, no. 2 (1985): 107–14. http://dx.doi.org/10.1139/o85-016.

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A triacylglycerol synthetase complex made up of acyl-CoA synthetase, acyl-CoA:monoacylglycerol acyltransferase, and acyl-CoA:diacylglycerol acyltransferase has been solubilized by sodium taurocholate and isolated by chromatography on phenyl-Sepharose. For this purpose microsomes of the villus cells of rat intestinal mucosa were dissolved in 2% sodium taurocholate prepared in 1 M (NH4)2SO4 and 25 mM Tris–HCl (pH 8.5) (buffer A). After dialysis against buffer A, the sample was loaded on a phenyl-Sepharose column and the enzyme complex was eluted with 25 mM Tris–HCl (pH 8.5) (buffer B). The enzym
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31

Lidholt, K., I. Eriksson, and L. Kjellén. "Heparin proteoglycans synthesized by mouse mastocytoma contain chondroitin sulphate." Biochemical Journal 311, no. 1 (1995): 233–38. http://dx.doi.org/10.1042/bj3110233.

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Proteoglycans (PGs), biosynthetically labelled with [35S]sulphate, were isolated from mouse mastocytoma tissue. Chromatography on antithrombin (AT)-Sepharose resulted in the separation of the 35S-labelled PGs into three fractions: PGs with no affinity for the gel (NA-PGs), PGs with low affinity (LA-PGs), and PGs with high affinity (HA-PGs) for antithrombin. Whereas NA-PGs contained almost exclusively chondroitin sulphate (CS), the AT-binding PGs contained 80-85% heparin and 15-20% CS. [35S]CS-containing macromolecules obtained from the HA-PG fraction after removal of the heparin polysaccharide
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32

Kirsch, C. M., and S. Samnick. "Einfache und schnelle Routineherstellung von ungeträgertem Meta-I-123- und I-131-Iodbenzylguanidin (I-123-MIBG und I-131-MIBG) für die klinischen nuklearmedizinischen Anwendungen." Nuklearmedizin 38, no. 07 (1999): 292–96. http://dx.doi.org/10.1055/s-0038-1632223.

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Zusammenfassung Ziel: Meta-Iodbenzylguanidin (I-123/I-131-MIBG) wird derzeit mit niedriger spezifischer Aktivität in der Erfassung neuroadrenerger Funktionsstörungen des Herzen sowie im Management neuroendokriner Tumoren eingesetzt. Jedoch wird intensiv diskutiert, ob beim Einsatz von ungeträgertem (n.c.a.) 1-123-MIBG beziehungsweise 1-131-MIBG ein diagnostischer beziehungsweise therapeutischer ZugewinnA/orteil zu erzielen ist - nicht zuletzt seit eine höhere und spezifischere Anreicherung des n.c.a. Radiopharmakons im Myokard und Tumorzellen berichtet wurde. In der vorliegenden Arbeit wird ei
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33

Okayama, E., K. Oguri, T. Kondo, and M. Okayama. "Isolation and characterization of chondroitin 6-sulfate proteoglycans present in the extracellular matrix of rabbit bone marrow." Blood 72, no. 2 (1988): 745–55. http://dx.doi.org/10.1182/blood.v72.2.745.745.

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Abstract The authors' previous studies showed that the glycosaminoglycans present in rabbit bone marrow were composed of chondroitin 6-sulfate (79%) and hyaluronic acid (16%). Immunohistochemically the chondroitin 6-sulfate was demonstrated to be in bone marrow matrix constructing hematopoietic microenvironment. In this study the authors isolated and characterized these glycosaminoglycans in their macromolecular form (i.e., proteoglycans). Bone marrow of 3-month-old rabbits was defatted with organic solvents containing proteinase inhibitors at -20 degrees C, and proteoglycans were extracted fr
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34

Okayama, E., K. Oguri, T. Kondo, and M. Okayama. "Isolation and characterization of chondroitin 6-sulfate proteoglycans present in the extracellular matrix of rabbit bone marrow." Blood 72, no. 2 (1988): 745–55. http://dx.doi.org/10.1182/blood.v72.2.745.bloodjournal722745.

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Abstract:
The authors' previous studies showed that the glycosaminoglycans present in rabbit bone marrow were composed of chondroitin 6-sulfate (79%) and hyaluronic acid (16%). Immunohistochemically the chondroitin 6-sulfate was demonstrated to be in bone marrow matrix constructing hematopoietic microenvironment. In this study the authors isolated and characterized these glycosaminoglycans in their macromolecular form (i.e., proteoglycans). Bone marrow of 3-month-old rabbits was defatted with organic solvents containing proteinase inhibitors at -20 degrees C, and proteoglycans were extracted from the de
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35

Landreth, K. S., and K. Dorshkind. "Pre-B cell generation potentiated by soluble factors from a bone marrow stromal cell line." Journal of Immunology 140, no. 3 (1988): 845–52. http://dx.doi.org/10.4049/jimmunol.140.3.845.

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Abstract Two bone marrow stromal cell lines isolated from the adherent layer of a Dexter-type long term bone marrow culture differ markedly in their hemopoietic support capacity. S17 supports myelopoiesis and the differentiation of early B cell precursors into B lymphocytes while S10 supports myeloid cell differentiation and not B lymphopoiesis. The identification of a stromal cell line with B cell support capacity prompted an investigation of whether the effects of S17 were mediated via soluble factors. Results presented herein indicate that medium conditioned by S17 but not S10 contains an a
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36

Clements, P. R., D. A. Brooks, P. A. G. McCourt та J. J. Hopwood. "Immunopurification and characterization of human α-l-iduronidase with the use of monoclonal antibodies". Biochemical Journal 259, № 1 (1989): 199–208. http://dx.doi.org/10.1042/bj2590199.

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alpha-L-Iduronidase from human liver was purified by a three-step five-column procedure and by immunoaffinity chromatography with a monoclonal antibody raised against purified enzyme. Seven bands identified by staining with Coomassie Blue had molecular masses of 74, 65, 60, 49, 44, 18 and 13 kDa and were present in both preparations of the liver enzyme. However, relative to the immunopurification procedure, alpha-L-iduronidase purified by the five-column procedure was considerably enriched in the 65 kDa polypeptide band. The seven bands were identified by Western-blot analysis with two differe
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37

da Silva, Marciele Souza, Valdirene Moreira Gomes, Gabriel Bonan Taveira, et al. "Bifunctional Inhibitors from Capsicum chinense Seeds with Antimicrobial Activity and Specific Mechanism of Action against Phytopathogenic Fungi." Protein & Peptide Letters 27 (June 17, 2020). http://dx.doi.org/10.2174/0929866527666200617124221.

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Background: Antimicrobial peptides (AMPs) are found in the defense system in virtually all life forms, being present in many, if not all, plant species. Objective: The present work evaluated the antimicrobial, enzymatic activity and mechanism of action of the PEF2 fraction from Capsicum chinense Jack. seeds against phytopathogenic fungi. Methods: Peptides were extracted from C. chinense seeds and subjected to reverse-phase chromatography on an HPLC system using a C18 column coupled to a C8 guard column, then the obtained PEF2 fraction was rechromatographed using a C2/C18 column. Two fractions,
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