Relevant bibliographies by topics / Recombinase protein

Contents

  1. Journal articles
  2. Dissertations / Theses
  3. Books
  4. Book chapters
  5. Conference papers
  6. Reports

Academic literature on the topic 'Recombinase protein'

Author: Grafiati
Published: 25 May 2024

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Journal articles on the topic "Recombinase protein"

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Price, Candice, and Isabel Darcy. "Application of a skein relation to difference topology experiments." Journal of Knot Theory and Its Ramifications 28, no. 13 (2019): 1940016. http://dx.doi.org/10.1142/s0218216519400169.

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Difference topology is a technique used to study any protein that can stably bind to DNA. This technique is used to determine the conformation of DNA bound by protein. Motivated by difference topology experiments, we use the skein relation tangle model as a novel technique to study experiments using topoisomerase to study SMC proteins, a family of proteins that stably bind to DNA. The oriented skein relation involves an oriented knot, [Formula: see text], with a distinguished positive crossing; a knot [Formula: see text], obtained by changing the distinguished positive crossing of [Formula: se
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Briones, Gabriel, Dirk Hofreuter, and Jorge E. Galán. "Cre Reporter System To Monitor the Translocation of Type III Secreted Proteins into Host Cells." Infection and Immunity 74, no. 2 (2006): 1084–90. http://dx.doi.org/10.1128/iai.74.2.1084-1090.2006.

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ABSTRACT Central to the study of type III secretion systems is the availability of reporter systems to monitor bacterial protein translocation into host cells. We report here the development of a bacteriophage P1 Cre recombinase-based system to monitor the translocation of bacterial proteins into mammalian cells. Bacteriophage P1 Cre recombinase fused to the secretion and translocation signals of Salmonella enterica serovar Typhimurium of the type III secreted protein SopE was secreted in a type III secretion system-dependent fashion. More importantly, the SopE-Cre chimera was translocated int
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Letunic, Ivica, Supriya Khedkar, and Peer Bork. "SMART: recent updates, new developments and status in 2020." Nucleic Acids Research 49, no. D1 (2020): D458—D460. http://dx.doi.org/10.1093/nar/gkaa937.

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Abstract SMART (Simple Modular Architecture Research Tool) is a web resource (https://smart.embl.de) for the identification and annotation of protein domains and the analysis of protein domain architectures. SMART version 9 contains manually curated models for more than 1300 protein domains, with a topical set of 68 new models added since our last update article (1). All the new models are for diverse recombinase families and subfamilies and as a set they provide a comprehensive overview of mobile element recombinases namely transposase, integrase, relaxase, resolvase, cas1 casposase and Xer l
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Marshall Stark, W., Martin R. Boocock, Femi J. Olorunniji, and Sally-J. Rowland. "Intermediates in serine recombinase-mediated site-specific recombination." Biochemical Society Transactions 39, no. 2 (2011): 617–22. http://dx.doi.org/10.1042/bst0390617.

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Site-specific recombinases are enzymes that promote precise rearrangements of DNA sequences. They do this by cutting and rejoining the DNA strands at specific positions within a pair of target sites recognized and bound by the recombinase. One group of these enzymes, the serine recombinases, initiates strand exchange by making double-strand breaks in the DNA of the two sites, in an intermediate built around a catalytic tetramer of recombinase subunits. However, these catalytic steps are only the culmination of a complex pathway that begins when recombinase subunits recognize and bind to their
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Orth, Peter, Petra Jekow, Juan C. Alonso та Winfried Hinrichs. "Proteolytic cleavage of Gram-positive β recombinase is required for crystallization". Protein Engineering, Design and Selection 12, № 5 (1999): 371–73. http://dx.doi.org/10.1093/protein/12.5.371.

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Chen, J. W., B. R. Evans, S. H. Yang, H. Araki, Y. Oshima, and M. Jayaram. "Functional analysis of box I mutations in yeast site-specific recombinases Flp and R: pairwise complementation with recombinase variants lacking the active-site tyrosine." Molecular and Cellular Biology 12, no. 9 (1992): 3757–65. http://dx.doi.org/10.1128/mcb.12.9.3757-3765.1992.

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The site-specific recombinases Flp and R from Saccharomyces cerevisiae and Zygosaccharomyces rouxii, respectively, are related proteins that belong to the yeast family of site-specific recombinases. They share approximately 30% amino acid matches and exhibit a common reaction mechanism that appears to be conserved within the larger integrase family of site-specific recombinases. Two regions of the proteins, designated box I and box II, also harbor a significantly high degree of homology at the nucleotide sequence level. We have analyzed the properties of Flp and R variants carrying point mutat
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Chen, J. W., B. R. Evans, S. H. Yang, H. Araki, Y. Oshima, and M. Jayaram. "Functional analysis of box I mutations in yeast site-specific recombinases Flp and R: pairwise complementation with recombinase variants lacking the active-site tyrosine." Molecular and Cellular Biology 12, no. 9 (1992): 3757–65. http://dx.doi.org/10.1128/mcb.12.9.3757.

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The site-specific recombinases Flp and R from Saccharomyces cerevisiae and Zygosaccharomyces rouxii, respectively, are related proteins that belong to the yeast family of site-specific recombinases. They share approximately 30% amino acid matches and exhibit a common reaction mechanism that appears to be conserved within the larger integrase family of site-specific recombinases. Two regions of the proteins, designated box I and box II, also harbor a significantly high degree of homology at the nucleotide sequence level. We have analyzed the properties of Flp and R variants carrying point mutat
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Koornneef, Lieke, Johan A. Slotman, Esther Sleddens-Linkels, et al. "Multi-color dSTORM microscopy in Hormad1-/- spermatocytes reveals alterations in meiotic recombination intermediates and synaptonemal complex structure." PLOS Genetics 18, no. 7 (2022): e1010046. http://dx.doi.org/10.1371/journal.pgen.1010046.

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Recombinases RAD51 and its meiosis-specific paralog DMC1 accumulate on single-stranded DNA (ssDNA) of programmed DNA double strand breaks (DSBs) in meiosis. Here we used three-color dSTORM microscopy, and a mouse model with severe defects in meiotic DSB formation and synapsis (Hormad1-/-) to obtain more insight in the recombinase accumulation patterns in relation to repair progression. First, we used the known reduction in meiotic DSB frequency in Hormad1-/- spermatocytes to be able to conclude that the RAD51/DMC1 nanofoci that preferentially localize at distances of ~300 nm form within a sing
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Meador, Kyle, Christina L. Wysoczynski, Aaron J. Norris, Jason Aoto, Michael R. Bruchas, and Chandra L. Tucker. "Achieving tight control of a photoactivatable Cre recombinase gene switch: new design strategies and functional characterization in mammalian cells and rodent." Nucleic Acids Research 47, no. 17 (2019): e97-e97. http://dx.doi.org/10.1093/nar/gkz585.

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AbstractA common mechanism for inducibly controlling protein function relies on reconstitution of split protein fragments using chemical or light-induced dimerization domains. A protein is split into fragments that are inactive on their own, but can be reconstituted after dimerization. As many split proteins retain affinity for their complementary half, maintaining low activity in the absence of an inducer remains a challenge. Here, we systematically explore methods to achieve tight regulation of inducible proteins that are effective despite variation in protein expression level. We characteri
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Dreyfus, David. "RAG-1 (Recombination Activating Gene-1) protein is closely related to herpes virus recombinases: Implications for the origins of the acquired immune system. (105.20)." Journal of Immunology 188, no. 1_Supplement (2012): 105.20. http://dx.doi.org/10.4049/jimmunol.188.supp.105.20.

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Abstract The acquired immune system results from recombination of germ-line DNA V(D)J signal sequences by the RAG-1 recombinase. RAG-1 is a dde magnesium binding recombinase related to both retroviral integrases and transposases. However the closest phylogenetic relative to RAG-1 appears to be a family of recombinases encoded by the herpesviridae termed the DBP (DNA binding proteins) that catalyze strand exchange and possibly other recombination events during herpes virus replication. Both the dde magnesium binding triad and the nonamer binding regions of RAG-1 are present and conserved in the
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Dissertations / Theses on the topic "Recombinase protein"

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Liu, Siyu. "Dynamics of Rad51 during homologous recombination in living yeast." Electronic Thesis or Diss., Université Paris sciences et lettres, 2022. http://www.theses.fr/2022UPSLS050.

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L'ADN est le principal vecteur d'information génétique et son intégrité est vitale pour la survie des cellules. Pourtant, l'ADN est sous la pression des dommages causés par des facteurs exogènes et endogènes. Les cassures double brin (CDB) sont parmi les dommages les plus toxiques puisqu’une CDB non réparée peut être léthale. Les cellules ont développé plusieurs voies pour réparer les CDB, dont la jonction d'extrémités non homologues (NHEJ) et la recombinaison homologue (RH). RH est une voie de réparation fidèle qui utilise une séquence homologue intacte comme modèle pour réparer les dommages.
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Zhekov, Ivailo. "Dissection of a functional interaction between the XerD recombinase and the DNA translocase FtsK." Thesis, University of Oxford, 2011. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.572642.

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Successful bacterial circular chromosome segregation requires that any dimeric chromosomes, which arise by crossing over during homologous recombination, are converted to monomers. Resolution of dimers to monomers requires the action of the XerCD site-specific recombinase at dif in the chromosome replication terminus region. This reaction requires the DNA translocase, FtsK(C), which activates dimer resolution by catalysing an ATP hydrolysis-dependent switch in the catalytic state of the nucleoprotein recombination complex. We show that a 62-amino-acid fragment of FtsK(C) interacts directly wit
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Corbett, Sybilla Louise. "Nanoscale patterning of complex DNA structures with the bacterial protein Recombinase A." Thesis, University of Leeds, 2016. http://etheses.whiterose.ac.uk/15373/.

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The use of DNA as a structural material has been intensively developed since its inception in the early 1980s. The potential of DNA structures in the field of materials science is hampered by current approaches to augmentation. It is not currently possible to alter the targeting of heterogenous additional elements to structures once they have been made. The post hoc patterning of DNA architectures is therefore of great importance. The bacterial protein Recombinase A (RecA) may be able to provide this function. This thesis will discuss the patterning of DNA structures with RecA. RecA has been s
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Yu, David Sung-wen. "Role of the BRCA2 breast cancer susceptibility protein in control of RAD51 recombinase." Thesis, University of Cambridge, 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.620033.

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Bates, D. L. "Control of the RAD51 recombinase by the BRC repeat motifs in the breast cancer susceptibility protein BRCA2." Thesis, University of Cambridge, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.596469.

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The interaction between the breast cancer susceptibility protein BRCA2 and the RAD51 DNA recombinase is essential for DNA repair via homologous recombination. Its disruption in human cells causes genomic instability and cancer predisposition. Studies to define the biochemical and biological features of the BRCA2:RAD51 interaction are described. Human BRCA2 features eight BRC repeat motifs encoded within exon 11 through which it can bind RAD51. The BRC repeat motifs are believed to mimic and disrupt RAD51 oligomerisation at its self-association interface. I defined the minimal structural determ
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Amero, Carlos D. "Protein Function Study by NMR Spectroscopy." The Ohio State University, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=osu1205431343.

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Ghorbal, Mehdi. "Caractérisation biochimique, fonctionnelle et structurale de l'integrase Pf-Int de plasmodium." Phd thesis, Université Paris Sud - Paris XI, 2012. http://tel.archives-ouvertes.fr/tel-00685428.

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Plasmodium falciparum est un parasite protozoaire responsable de la forme la plus sévère de la malaria. Depuis quelques années, les cas de résistance aux antipaludiques sont devenus de plus en plus fréquents et de plus en plus répandus. En plus de sa résistance aux drogues actuellement disponibles, ce parasite reste jusqu' à aujourd'hui réfractaire aux vaccinations. L'identification de nouvelles approches basées sur l'inhibition spécifique de certaines de ses cibles moléculaires vitales est devenue une nécessité. La recombinase à site spécifique de P. falciparum (Pf-Int) est un enzyme qui a ét
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Koscky, Paier Carlos Roberto 1983. "Padronização da expressão heterologa e de modelo de ensaio de atividade para a proteina quinase humana S6K." [s.n.], 2009. http://repositorio.unicamp.br/jspui/handle/REPOSIP/314787.

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Orientador: Nilson Ivo Tonin Zanchin<br>Dissertação (mestrado) - Universidade Estadual de Campinas, Instituto de Biologia<br>Made available in DSpace on 2018-08-14T12:40:52Z (GMT). No. of bitstreams: 1 KosckyPaier_CarlosRoberto_M.pdf: 3760581 bytes, checksum: 99331529324819b59a4360d60efd9b9a (MD5) Previous issue date: 2009<br>Resumo: A quinase de 70 kDa da proteína ribossomal S6, isoforma 1 (S6K1), é uma fosfoproteína implicada na regulação de genes relacionados ao controle da tradução em mamíferos e possui uma forma nuclear (a1) e uma citoplasmática (a2). A fosforilação do seu principal alv
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Lu, Yang. "Functional studies of new protein-protein interactions potentially involved in homologous recombination in hyperthermophilic archaea : study of interactions between PCNA and Mre11-Rad50 complex & Primase and RadA." Thesis, Brest, 2018. http://www.theses.fr/2018BRES0077/document.

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Les archées hyperthermophiles ont une température optimale de croissance supérieure à 80°C.Les cellules exposées à un stress thermique subissent une augmentation de la sensibilité aux agents induisant des cassures double brin de l’ADN. Les études sur les eucaryotes et bactéries ont démontré que la recombinaison homologue joue un rôle essentiel non seulement dans la réparation de l’ADN, mais aussi dans le redémarrage des arrêts de la fourche de réplication. Les enzymes associées aux étapes initiales de la recombinaison homologue chez les archées sont homologues à celles des eucaryotes, et diffé
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Corgozinho, Carolina Nunes Costa. "Desenvolvimento de vacina baseada em sistema de liberação sustentada contendo proteína recombinante." Universidade de São Paulo, 2008. http://www.teses.usp.br/teses/disponiveis/60/60137/tde-31072009-083709/.

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No Brasil, e em outros paises de clima tropical, os carrapatos se tornaram um enorme problema economico de^$de que a industria do gado se desenvolveu. O carrapato Boophilus microplus, um dos artropodes mais importantes na veterinaria, causa efeitos direto, como suc,cao de sangue, e indireto, como a transmissao de uma grande variedade de patogenos que normalmente resulta em infec,c~es letais. As vacinas genicas contendo o antigeno Bm86, uma proteina ligada a membrana do intestino do carrapato B. microplus, representam uma alternativa atrativa aos acaricidas para controlar as infesta~oes por car
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Books on the topic "Recombinase protein"

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Jean, Garnier, ed. Introduction to proteins and protein engineering. Elsevier, 1988.

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Jean, Garnier, ed. Introduction to proteins and protein engineering. Elsevier, 1986.

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1961-, Castro Fidel O., and Jänne Juhani, eds. Mammary gland transgenesis: Therapeutic protein production. Springer, 1998.

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Robson, Barry. Introductionto proteins and protein engineering. Elsevier, 1988.

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Tuan, Rocky S. Recombinant Protein Protocols. Humana Press, 1997. http://dx.doi.org/10.1385/089603481x.

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Buckel, Peter, ed. Recombinant Protein Drugs. Birkhäuser Basel, 2001. http://dx.doi.org/10.1007/978-3-0348-8346-7.

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Robson, Barry. Introduction to proteins and protein engineering. Elsevier, 1986.

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1947-, Stein Stanley, ed. Fundamentals of protein biotechnology. M. Dekker, 1990.

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Bill, Roslyn M., ed. Recombinant Protein Production in Yeast. Humana Press, 2012. http://dx.doi.org/10.1007/978-1-61779-770-5.

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Gasser, Brigitte, and Diethard Mattanovich, eds. Recombinant Protein Production in Yeast. Springer New York, 2019. http://dx.doi.org/10.1007/978-1-4939-9024-5.

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Book chapters on the topic "Recombinase protein"

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Buntru, Matthias, Simon Vogel, Ricarda Finnern, and Stefan Schillberg. "Plant-Based Cell-Free Transcription and Translation of Recombinant Proteins." In Recombinant Proteins in Plants. Springer US, 2022. http://dx.doi.org/10.1007/978-1-0716-2241-4_8.

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AbstractPlant cell-free lysates contain all the cellular components of the protein biosynthesis machinery, providing an alternative to intact plant cells, tissues, and whole plants for the production of recombinant proteins. Cell-free lysates achieve rapid protein production (within hours or days) and allow the synthesis of proteins that are cytotoxic or unstable in living cells. The open nature of cell-free lysates and their homogeneous and reproducible performance is ideal for protein production, especially for screening applications, allowing the direct addition of nucleic acid templates encoding proteins of interest, as well as other components such as enzyme substrates, chaperones, artificial amino acids, or labeling molecules. Here we describe procedures for the production of recombinant proteins in the ALiCE (Almost Living Cell-free Expression) system, a lysate derived from tobacco cell suspension cultures that can be used to manufacture protein products for molecular and biochemical analysis as well as applications in the pharmaceutical industry.
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Maetzig, Tobias, and Axel Schambach. "Development of Inducible Molecular Switches Based on All-in-One Lentiviral Vectors Equipped with Drug Controlled FLP Recombinase." In Lentiviral Vectors and Exosomes as Gene and Protein Delivery Tools. Springer New York, 2016. http://dx.doi.org/10.1007/978-1-4939-3753-0_2.

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Fothergill-Gilmore, Linda A. "Recombinant Protein Technology." In Protein Biotechnology. Humana Press, 1993. http://dx.doi.org/10.1007/978-1-59259-438-2_13.

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Weissmann, Charles. "Recombinant interferon - the 20th anniversary." In Recombinant Protein Drugs. Birkhäuser Basel, 2001. http://dx.doi.org/10.1007/978-3-0348-8346-7_1.

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Hofschneider, Peter Hans, and Kenneth Murray. "Combining science and business: from recombinant DNA to vaccines against hepatitis B virus." In Recombinant Protein Drugs. Birkhäuser Basel, 2001. http://dx.doi.org/10.1007/978-3-0348-8346-7_2.

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Brownlee, George G., and Paul L. F. Giangrande. "Clotting factors VIII and IX." In Recombinant Protein Drugs. Birkhäuser Basel, 2001. http://dx.doi.org/10.1007/978-3-0348-8346-7_3.

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Welte, Karl, and Erich Platzer. "Colony-stimulating factors: altering the practice of oncology." In Recombinant Protein Drugs. Birkhäuser Basel, 2001. http://dx.doi.org/10.1007/978-3-0348-8346-7_4.

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Collen, Désiré, and H. Roger Lijnen. "Tissue-type plasminogen activator: helping patients with acute myocardial infarction." In Recombinant Protein Drugs. Birkhäuser Basel, 2001. http://dx.doi.org/10.1007/978-3-0348-8346-7_5.

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Gillies, Stephen D. "Designing immunocytokines: genetically engineered fusion proteins for targeted immune therapy." In Recombinant Protein Drugs. Birkhäuser Basel, 2001. http://dx.doi.org/10.1007/978-3-0348-8346-7_6.

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Burke, Paul A., and Scott D. Putney. "Improving protein therapeutics: the evolution of the modern pharmacopoeia." In Recombinant Protein Drugs. Birkhäuser Basel, 2001. http://dx.doi.org/10.1007/978-3-0348-8346-7_7.

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Conference papers on the topic "Recombinase protein"

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Berkner, K. L., S. J. Busby, J. Gambee, and A. Kumar. "EXPRESSION IN MAMMALIAN CELLS OF FUSION PROTEINS BETWEEN HUMAN FACTORS IX AND VII." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643568.

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The vitamin K-dependent plasma proteins demonstrate remarkable similarities in their structures: all have multiple domains in common and extensive homology is observed within many of these domains. In order to investigate the structure-function relationship of these proteins, we have interchanged domains of one protein (factor IX) with that of another (factor VII) and have compared the expression of these fusion proteins with recombinant and native factors IX and VII. Oligonucleotide-directed mutagenesis was used to generate four fusion proteins: factor IX/VII-1, which contains the factor IX l
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Tyumentsev, A. I., M. A. Tyumentseva, and V. G. Akimkin. "DEVELOPMENT OF APPROACHES FOR ENDOTOXIN REMOVAL FROM PROTEIN PREPARATIONS ON THE EXAMPLE OF NUCLEASES OF THE CRISPR/CAS SYSTEM." In Molecular Diagnostics and Biosafety. Federal Budget Institute of Science 'Central Research Institute for Epidemiology', 2020. http://dx.doi.org/10.36233/978-5-9900432-9-9-113.

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Removal of bacterial endotoxins from solutions of recombinant proteins is one of the most important issues in the preparation of highly purified preparations suitable for in vivo use. An optimal technology for obtaining preparations purified from bacterial endotoxins has been proposed using purification of preparations of recombinant nucleases of the CRISPR/CAS system as an example. Efficacy of developed technology was compared with other available methods. Removal of bacterial endotoxins was carried out using Triton X-114 detergent added to a concentration of 1% to a solution containing the r
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Neklesova, M. V., and N. Deeb. "PRODUCTION OF RECOMBINANT PROTEINS AMUC_1100 AND P9 AND ASSESSMENT OF THEIR IN VITRO ANTITUMOR ACTIVITY." In X Международная конференция молодых ученых: биоинформатиков, биотехнологов, биофизиков, вирусологов и молекулярных биологов — 2023. Novosibirsk State University, 2023. http://dx.doi.org/10.25205/978-5-4437-1526-1-106.

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The probiotic properties of Akkermansia muciniphila in the treatment of metabolic, cardiovascular and oncological diseases are mediated by the surface protein Amuc_1100 (Amuc) and secreted protein P9, but their combined effect has not been investigated. In this work, a recombinant producer E. coli BL21(DE3) of proteins Amuc and P9 was obtained. It is planned to conduct experiments on the colorectal cancer cell line using a combination of Amuc and P9.
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Vehar, G. A. "THE PRESENT STATE OF GENE TECHNOLOGY IN THE MANUFACTURE OF HUMAN COAGULATION PROTEINS." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644755.

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The production of pharmaceuticals from human plasma that are useful in the treatment of bleeding disorders had its beginning with the development of the Cohn fractionation procedure in the 1940's. As a result of these advances, concentrates became available for the treatment of the hemophilias. Although of low purity and subject to contamination by hepatitis virus, the availability of these compounds resulted in dramatic improvements in the life expectancy and quality of life of afflicted individuals. The numerous problems associated with production of pharmaceuticals from pooled plasma made t
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Ashok Kumar, A., Margaret Insley, Jay Gambee, Sharon J. Busby, and Kathleen L. Berkner. "SITE SPECIFIC MUTAGENESIS WITHIN THE GLA-DOMAIN OF HUMAN FACTOR IX." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644079.

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Factor IX, a plasma protein, plays a critical role in blood coagulation. The biological activity of factor IX as well as several other plasma proteins depends on the presence of gamma-carboxy glutamic acid (Gla) residues in their amino terminal region. In vitro mutagenesis has been used to selectively replace Gla residues of factor IX with aspartic acid (Asp) residues in order to establish the contribution of individual as well as paired Gla residues to the normal functioning of the protein. These substitutions were made at positions 7, 15, 20 and 26 in human factor IX. In addition, residue nu
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Marchenko, D. M., M. S. Bozhokin, E. R. Mikhailova, Yu V. Sopova, and M. G. Khotin. "STIMULATION OF HUMAN DERMAL FIBROBLASTS CHONDROGENIC DIFFERENTIATION FOR TISSUE ENGINEERING OF HYALINE CARTILAGE." In X Международная конференция молодых ученых: биоинформатиков, биотехнологов, биофизиков, вирусологов и молекулярных биологов — 2023. Novosibirsk State University, 2023. http://dx.doi.org/10.25205/978-5-4437-1526-1-101.

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This work is devoted to the possibility of human dermal fibroblasts chondrogenic differentiation using the TGF-β3 and SOX9 recombinant proteins and lentiviral vectors carrying sequences of the Tgf-β3 and Sox9 genes. It has been shown that the use of the TGF-β3 protein and vector with the Sox9 gene makes it possible to increase the expression of associated with chondrogenesis genes in fibroblasts.
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Furis, B. C., M. J. Jorgensem, M. J. Rabiet, et al. "RECOGNITION SITE DIRECTING GAMMA-CARBOXYLATION RESIDES ON THE PROPEPTIDES OF FACTOR IX AND PROTRROMBIN." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643992.

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Factor IX and prothrombin vitamin K-dependent proteins that participate in blood coagulation undergo post-translationalmodification in which glutamic acid residues in the amino terminus of the protein are converted to gamma-carboxyglutamic acid residues. This modification confers divalent metal ion binding ability upon the proteins.As a consequence of binding divalent metal ions these proteins undergoconformational changes necessary for biological function.The vitamin K-dependent proteins are synthesized with an NH2-terminal extension. The region distal to the NH2-terminus of the mature protei
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Novikova, L. I., S. S. Bochkareva, A. V. Aleshkin, et al. "DYNAMICS OF ANTIBODIES TO VARIOUS ANTIGENS OF THE SARS-COV-2 CORONAVIRUS IN PATIENTS WITH CONFIRMED COVID-19 INFECTION." In Molecular Diagnostics and Biosafety. Federal Budget Institute of Science 'Central Research Institute for Epidemiology', 2020. http://dx.doi.org/10.36233/978-5-9900432-9-9-159.

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Presence of IgG and IgM antibodies in venous blood of 76 patients with confirmed presence of SARS-CoV-2 was determined. The study was carried out by ELISA using Russian test systems. Revealed different levels of IgM antibodies to N-protein and RBD (receptor binding domain of the Spike protein). The level of IgM to RBD did not reach high values, while the level of IgM to N-protein sharply increased in a short period of time to high values by the 3rd week of the disease and decreased only by the 8th week. The dynamics of IgG antibodies to the whole virion antigen and the recombinant spikes was s
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Teng, Weibing, Yiding Huang, Joseph Cappello, and Xiaoyi Wu. "Mechanical and In-Vitro Cell Compatibility Properties of Silk-Elastinlike Protein-Based Biomaterial." In ASME 2010 First Global Congress on NanoEngineering for Medicine and Biology. ASMEDC, 2010. http://dx.doi.org/10.1115/nemb2010-13141.

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A series of genetically engineered recombinant silk-elastinlike proteins (SELPs) have been produced by combining polypeptide sequences derived from native silk of superior mechanical strength and elastin that is extremely durable and resilient. They have displayed a set of outstanding properties such as good biocompatibility and controllable biodegradation rates. In the study, we characterized the mechanical property of genetically engineered, recombinant silk-elastinlike protein copolymer, SELP-47K, under physical and chemical treatments. The biocompatibility of the SELP-47K was also evaluate
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Mikhaylova, E. E., I. K. Baykov, and N. V. Tikunova. "MODIFICATION OF AN ANTIBODY AGAINST TICK-BORNE ENCEPHALITIS VIRUS USING DIRECTED PROTEIN EVOLUTION." In X Международная конференция молодых ученых: биоинформатиков, биотехнологов, биофизиков, вирусологов и молекулярных биологов — 2023. Novosibirsk State University, 2023. http://dx.doi.org/10.25205/978-5-4437-1526-1-348.

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It is possible to obtain modified protein molecules with the properties necessary for the researcher by various methods. One of them is the directed evolution of proteins, which mimics the process of natural selection. The use of this method allowed to increase the affinity of the single-stranded antibody sc14D5 to the recombinant domain III of glycoprotein E of the European and Siberian subtypes of tick-borne encephalitis virus.
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Reports on the topic "Recombinase protein"

1

Hodges, Thomas K., and David Gidoni. Regulated Expression of Yeast FLP Recombinase in Plant Cells. United States Department of Agriculture, 2000. http://dx.doi.org/10.32747/2000.7574341.bard.

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Research activities in both our laboratories were directed toward development of control of the FLP/frt recombination system for plants. As described in the text of the research proposal, the US lab has been engaged in developing regulatory strategies such as tissue-specific promoters and the steroid-inducible activation of the FLP enzyme while the main research activities in Israel have been directed toward the development and testing of a copper-regulated expression of flp recombinase in tobacco (this is an example of a promoter activation by metal ions). The Israeli lab hat additionally com
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Banai, Menachem, and Gary Splitter. Molecular Characterization and Function of Brucella Immunodominant Proteins. United States Department of Agriculture, 1993. http://dx.doi.org/10.32747/1993.7568100.bard.

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The BARD project was a continuation of a previous BARD funded research project. It was aimed at characterization of the 12kDa immunodominant protein and subsequently the cloning and expression of the gene in E. coli. Additional immunodominant proteins were sought among genomic B. abortus expression library clones using T-lymphocyte proliferation assay as a screening method. The 12kDa protein was identified as the L7/L12 ribosomal protein demonstrating in the first time the role a structural protein may play in the development of the host's immunity against the organism. The gene was cloned fro
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Galili, Gad, and Alan Bennett. Role of Molecular Chaperone in Wheat Storage Protein Assembly. United States Department of Agriculture, 1995. http://dx.doi.org/10.32747/1995.7604926.bard.

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Following sequestration into the ER, wheat gliadins assemble into complexes that initiate the formation of protein bodies. In the present work we have characterized the DNA sequence and regulation of expression of a plant BiP and also studied its interaction with wheat storage proteins as well as its role in the maturation of these storage proteins. In the Israeli lab, immunoprecipitation studies were made using anti BiP and anti storage proteins sera, both in wheat and in transgenic tobacco plants expressing a wheat gliadin storage proteins. In both cases, we could show that BiP interacts wit
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Bercovier, Herve, Raul Barletta, and Shlomo Sela. Characterization and Immunogenicity of Mycobacterium paratuberculosis Secreted and Cellular Proteins. United States Department of Agriculture, 1996. http://dx.doi.org/10.32747/1996.7573078.bard.

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Our long-term goal is to develop an efficient acellular vaccine against paratuberculosis based on protein antigen(s). A prerequisite to achieve this goal is to analyze and characterize Mycobacterium paratuberculosis (Mpt) secreted and cellular proteins eliciting a protective immune response. In the context of this general objective, we proposed to identify, clone, produce, and characterize: the Mpt 85B antigen and other Mpt immunoreactive secreted proteins, the Mpt L7/L12 ribosomal protein and other immunoreactive cellular proteins, Mpt protein determinants involved in invasion of epithelial c
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Splitter, Gary, Zeev Trainin, and Ruth Meirom. Effector Cell Recognition of Recombinant BHV-1 Proteins. United States Department of Agriculture, 1994. http://dx.doi.org/10.32747/1994.7604294.bard.

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Veen, Ryan Vander, Mark Mogler, Matthew M. Erdman, and D. L. Hank Harris. Preparation of GP5-M Heterodimer Glycantype Specific Recombinant Protein and Replicon Particles. Iowa State University, 2009. http://dx.doi.org/10.31274/ans_air-180814-698.

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Gafny, Ron, A. L. N. Rao, and Edna Tanne. Etiology of the Rugose Wood Disease of Grapevine and Molecular Study of the Associated Trichoviruses. United States Department of Agriculture, 2000. http://dx.doi.org/10.32747/2000.7575269.bard.

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Rugose wood is a complex disease of grapevines, characterized by modification of the woody cylinder of affected vines. The control of rugose wood is based on the production of healthy propagation material. Detection of rugose wood in grapevines is difficult and expensive: budwood from tested plants is grafted onto sensitive Vitis indicators and the appearance of symptoms is monitored for 3 years. The etiology of rugose wood is complex and has not yet been elucidated. Several elongated clostero-like viruses are consistently found in affected vines; one of them, grapevine virus A (GVA), is close
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Palmer, Guy H., Eugene Pipano, Terry F. McElwain, Varda Shkap, and Donald P. Knowles, Jr. Development of a Multivalent ISCOM Vaccine against Anaplasmosis. United States Department of Agriculture, 1993. http://dx.doi.org/10.32747/1993.7568763.bard.

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Anaplasmosis is an arthropod+borne disease of cattle caused by the rickettsia Anaplasma marginale and an impediment to efficient production of healthy livestock in both Israel and the United States. Our research focuses on development of a recombinant membrane surface protein (MSP) immunogen to replace current vaccines derived from the blood of infected cattle. The risk of widespread transmission of both known and newly emergent pathogens has prevented licensure of live blood-based vaccines in the U.S. and is a major concern for their continued use in Israel. Briefly, we accomplished the follo
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Walls, Lichun H. Isolation and Preliminary Characterization of a Recombinant TAT Protein From Human Immunodeficiency Virus. Defense Technical Information Center, 1995. http://dx.doi.org/10.21236/ada298304.

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Brayton, Kelly A., Varda Shkap, Guy H. Palmer, Wendy C. Brown, and Thea Molad. Control of Bovine Anaplasmosis: Protective Capacity of the MSP2 Allelic Repertoire. United States Department of Agriculture, 2014. http://dx.doi.org/10.32747/2014.7699838.bard.

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Anaplasmosis is an arthropod-borne disease of cattle caused by the rickettsia Anaplasmamarginale and is an impediment to efficient production of healthy livestock in both Israel and the United States. Currently, the only effective vaccines are derived from the blood of infected cattle. The risk of widespread transmission of both known and newly emergent pathogens has prevented licensure of live blood-based vaccines in the U.S. and is a major concern for their continued use in Israel. Consequently, development of a safe, effective vaccine is a high priority. Despite its drawbacks as a live, blo
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