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Relevant bibliographies by topics / Related protein identification

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Academic literature on the topic 'Related protein identification'

Author: Grafiati

Published: 4 June 2021

Last updated: 13 February 2022

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Journal articles on the topic "Related protein identification"

1

Coleman, Matthew A., Kristi A. Miller, Peter T. Beernink, Daniel M. Yoshikawa, and Joanna S. Albala. "Identification of chromatin-related protein interactions using protein microarrays." PROTEOMICS 3, no. 11 (2003): 2101–7. http://dx.doi.org/10.1002/pmic.200300593.

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Du, Haibo, Rui Ren, Panpan Chen, Zhigang Xu, and Yanfei Wang. "Identification of Binding Partners of Deafness-Related Protein PDZD7." Neural Plasticity 2018 (2018): 1–10. http://dx.doi.org/10.1155/2018/2062346.

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PDZD7 is an important deafness gene, whose mutations are associated with syndromic and nonsyndromic hearing loss. PDZD7 contains multiple PDZ domains that are essential for organizing various proteins into protein complex. Several PDZD7-binding proteins have been identified, including usherin, ADGRV1, whirlin, harmonin, SANS, and MYO7A, all belonging to USH proteins. Here, we report the identification of novel PDZD7-binding partners through yeast two-hybrid screening using the first two PDZ domains of PDZD7 as bait. Eleven proteins were identified, most of which have not been reported as PDZD7

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Saqi, Mansoor A. S., and Michael J. E. Sternberg. "Identification of sequence motifs from a set of porteins with related function." "Protein Engineering, Design and Selection" 7, no. 2 (1994): 165–71. http://dx.doi.org/10.1093/protein/7.2.165.

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Zhang, Ren-wen, Yong Qiao, Xiao-hua Hao, et al. "Identification of Human Hepatocyte Proliferation Related Gene C2orf69." Infection International 3, no. 1 (2014): 1–9. http://dx.doi.org/10.1515/ii-2017-0066.

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Abstract Objective To construct the prokaryotic expression vector pET-32a(+)-C2orf69 and induce the expression of recombinant proteins in vitro. Then the possible effects of recombinant protein on cell proliferation was observed and rabbit-anti-C2orf69 protein polyclonal antibodies was obtained. Methods Gene fragment of C2orf69 was amplified by PCR and then prokaryotic expression plasmid pET-32a(+)-C2orf69 was constructed. Recombinant protein C2orf69 expression was identified by SDS-PAGE and Western blot. The white-ear rabbits were immunized with purified recombinant protein C2orf69, and the p

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Conlan, L. A., T. J. Martin, and M. T. Gillespie. "Identification of protein interactors with parathyroid hormone-related protein (PTHrP)." Bone 27, no. 4 (2000): 14. http://dx.doi.org/10.1016/s8756-3282(00)80037-x.

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Matsuo, Yoshiyuki, Nobutake Akiyama, Hajime Nakamura, Junji Yodoi, Makoto Noda, and Shinae Kizaka-Kondoh. "Identification of a Novel Thioredoxin-related Transmembrane Protein." Journal of Biological Chemistry 276, no. 13 (2001): 10032–38. http://dx.doi.org/10.1074/jbc.m011037200.

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Tassin, A. M., C. Celati, M. Paintrand, and M. Bornens. "Identification of an Spc110p-related protein in vertebrates." Journal of Cell Science 110, no. 20 (1997): 2533–45. http://dx.doi.org/10.1242/jcs.110.20.2533.

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Although varying in size and complexity, centrosomes have conserved functions throughout the evolutionary range of eukaryotes, and thus may display conserved components. In this work, we took advantage of the recent advances in the isolation of the budding yeast spindle pole body, the development of specific immunological probes and the molecular characterisation of genes involved in spindle pole body duplication or assembly. Screening a monoclonal antibody library against Saccharomyces cerevisiae spindle pole body components, we found that two monoclonal antibodies, directed against two diffe

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Roby, K. F., S. Deb, G. Gibori, et al. "Decidual prolactin-related protein. Identification, molecular cloning, and characterization." Journal of Biological Chemistry 268, no. 5 (1993): 3136–42. http://dx.doi.org/10.1016/s0021-9258(18)53669-3.

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Khurana, T. S., E. P. Hoffman, and L. M. Kunkel. "Identification of a chromosome 6-encoded dystrophin-related protein." Journal of Biological Chemistry 265, no. 28 (1990): 16717–20. http://dx.doi.org/10.1016/s0021-9258(17)44816-2.

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Frankel, Stewart, Matthew B. Heintzelman, Spyridon Artavanis-Tsakonas, and Mark S. Mooseker. "Identification of a Divergent Actin-related Protein in Drosophila." Journal of Molecular Biology 235, no. 4 (1994): 1351–56. http://dx.doi.org/10.1006/jmbi.1994.1090.

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Dissertations / Theses on the topic "Related protein identification"

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Matsuo, Yoshiyuki. "Identification of a Novel Thioredoxin-related Transmembrane Protein." Kyoto University, 2001. http://hdl.handle.net/2433/150552.

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Lodge, Anthony Paul. "Identification of a novel protein related to HnRNP-U." Thesis, University of Liverpool, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.243247.

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Zhao, Qian, and 赵倩. "Identification of a binding target of triptolide and related studies." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2012. http://hub.hku.hk/bib/B48199163.

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Triptolide, a diterpene triepoxide extracted from traditional Chinese medicinal herb Tripterygium wilfordii Hook. F has been shown to have profound inhibitory effects against tumor progression, pathological angiogenesis and inflammation. However, the mechanisms by which triptolide exerts these effects remain unclear. To understand its cellular mode of action, biotinylated/desthiobiotinylated and fluorophore-labeled triptolide derivatives were used as probes to identify cellular proteins that bind to triptolide. By using two different approaches for screening drug-protein interactions, the m

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Hon, Jiří. "Vyhledávání příbuzných proteinů s modifikovanou funkcí." Master's thesis, Vysoké učení technické v Brně. Fakulta informačních technologií, 2015. http://www.nusl.cz/ntk/nusl-234914.

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Protein engineering is a young dynamic discipline with great amount of potential practical applications. However, its success is primarily based on perfect knowledge and usage of all existing information about protein function and structure. To achieve that, protein engineering is supported by plenty of bioinformatic tools and analysis. The goal of this project is to create a new tool for protein engineering that would enable researchers to identificate related proteins with modified function in still growing biological databases. The tool is designed as an automated workflow of existing bioin

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Neels, Jacobus Gerardus. "LDL receptor-related protein molecular analysis and identification of new ligands /." [S.l. : Amsterdam : s.n.] ; Universiteit van Amsterdam [Host], 2001. http://dare.uva.nl/document/60201.

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Nobre, da Cruz I. M. "Method development approaches towards the identification and validation of disease-related protein targets." Thesis, University College London (University of London), 2014. http://discovery.ucl.ac.uk/1433951/.

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The search for novel biomarkers and therapeutic targets is a continuous process in the fight against disease. In this project, target identification and validation approaches were used towards the discovery of protein targets related to distinct, but equally relevant diseases. The first approach involved the use of proteomics to identify protein targets in infertility. An affinity-enrichment proteomic method was developed and successfully applied to the identification of protein targets of a clinically utilised compound, which is known to cause reversible, dose-dependent male infertility in ce

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Randall, Susan. "Interactions among the mitogen-activated protein kinase cascades and the identification of a novel cdc2-related protein kinase." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp04/mq29271.pdf.

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Ananieva-Stoyanova, Elitsa Antonova. "Identification and Functional Role of Myo-Inositol Polyphosphate 5-Phosphatase Protein Complexes." Diss., Virginia Tech, 2009. http://hdl.handle.net/10919/28028.

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To survive, an organism must constantly adjust its internal state to changes in the environment from which it receives signals. The signals set off a chain of events referred to signal transduction. Signal transduction systems are especially important in multicellular organisms, such as plants and animals, because of the need to coordinate the activities of hundreds to trillions of cells. Plants, in particular, have a special need for perceiving signals from their environment because of their static nature. As in the animal cell, the first steps in perception of a signal include signal interac

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Rodríguez, Solovey Leisa Natacha. "IDENTIFICATION OF TARGETS AND AUXILIARY PROTEINS OF PYR/PYL/RCAR ABA RECEPTORS: PROTEIN PHOSPHATASES TYPE 2C (PP2Cs) AND C2-DOMAIN ABA-RELATED PROTEINS (CARs)." Doctoral thesis, Universitat Politècnica de València, 2015. http://hdl.handle.net/10251/58862.

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[EN] ABSTRACT Abscisic acid (ABA) signaling plays a critical role in regulating root growth and root system architecture. ABA-mediated growth promotion and root tropic response under water stress are key responses for plant survival under limiting water conditions. In this work, we have explored the role of Arabidopsis (Arabidopsis thaliana) PYR/PYL/RCAR receptors (PYRABACTIN RESISTANCE1 (PYR1)/PYR1 LIKE (PYL)/REGULATORY COMPONENTS OF ABA RECEPTORS) for root ABA signaling. As a result, we discovered that PYL8 plays a nonredundant role for the regulation of root ABA sensitivity. Unexpectedly,

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Marchal-Duval, Emmeline. "Identification de PRRX1 (Paired Related Homeobox Protein-1), un nouveau facteur de transcription impliqué dans la Fibrose Pulmonaire Idiopathique." Thesis, Sorbonne Paris Cité, 2018. http://www.theses.fr/2018USPCC326.

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La Fibrose Pulmonaire Idiopathique (FPI), est une maladie pulmonaire chronique, sans aucune thérapeutique curative à l’heure actuelle. Les fibroblastes sont les cellules clés du développement de la fibrose. D’origine mésenchymateuse, la régulation de leur phénotype est toujours assez peu comprise et parmi les régulateurs possibles, peu de facteurs de transcription (FT) spécifiques de ces cellules ont été découverts. Nos travaux mettent en évidence pour la première fois, l’implication du FT mésenchymateux PRRX1 dans la régulation du comportement aberrant des fibroblastes et dans le développemen

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Books on the topic "Related protein identification"

1

Baines-Pinchen, David Adam. Identification of a stress-related protein in the pea-powdery mildew interaction. University of Birmingham, 2001.

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2

Randall, Susan. Interactions among the mitogen-activated protein kinase cascades and the identification of a novel cdc2-related protein kinase. National Library of Canada = Bibliothèque nationale du Canada, 1999.

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Midmer, Michael. Identification of NKIAMRE, the human homologue to the MAPK/CDK-related protein kinase NKIATRE, and its loss in leukemic blasts with 5q-syndrome. National Library of Canada, 1999.

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Gans, Evelien, and Remco Ensel, eds. The Holocaust, Israel and 'the Jew'. Amsterdam University Press, 2016. http://dx.doi.org/10.5117/9789089648488.

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This book is the first comprehensive study of postwar antisemitism in the Netherlands. It focuses on the way stereotypes are passed on from one decade to the next, as reflected in public debates, the mass media, protests and commemorations, and everyday interactions. The Holocaust, Israel and 'the Jew' explores the ways in which old stories and phrases relating to 'the stereotypical Jew' are recycled and modified for new uses, linking the antisemitism of the early postwar years to its enduring manifestations in today's world. The Dutch case is interesting because of the apparent contrast betwe

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Gans, Evelien, and Remco Ensel, eds. The Holocaust, Israel and 'the Jew'. Amsterdam University Press, 2017. http://dx.doi.org/10.5117/9789462986084.

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This book is the first comprehensive study of postwar antisemitism in the Netherlands. It focuses on the way stereotypes are passed on from one decade to the next, as reflected in public debates, the mass media, protests and commemorations, and everyday interactions. The Holocaust, Israel and 'the Jew' explores the ways in which old stories and phrases relating to 'the stereotypical Jew' are recycled and modified for new uses, linking the antisemitism of the early postwar years to its enduring manifestations in today's world. The Dutch case is interesting because of the apparent contrast betwe

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Structure-function analysis of the immediate early transcription factor Egr-1 and identification of a gene repressed by a related zinc finger protein, the Wilms tumor suppressor WT1. 1992.

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7

Meng, X. J. Hepatitis E virus. Oxford University Press, 2011. http://dx.doi.org/10.1093/med/9780198570028.003.0048.

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Hepatitis E virus (HEV) is a small, non-enveloped, single-strand, positive-sense RNA virus of approximately 7.2 kb in size. HEV is classified in the family Hepeviridae consisting of four recognized major genotypes that infect humans and other animals. Genotypes 1 and 2 HEV are restricted to humans and often associated with large outbreaks and epidemics in developing countries with poor sanitation conditions, whereas genotypes 3 and 4 HEV infect humans, pigs and other animal species and are responsible for sporadic cases of hepatitis E in both developing and industrialized countries. The avian

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Vincent, Angela. Neuroimmunology. Oxford University Press, 2015. http://dx.doi.org/10.1093/med/9780199658602.003.0015.

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This chapter relates to antibody-mediated disorders throughout the nervous system. Early papers recall how use of bungarotoxin, passive transfer experiments in mice, and clinical response to plasma exchange confirmed the role of acetylcholine receptor antibodies in myasthenia gravis. Cutting edge techniques subsequently discovered other key neuromuscular junctional proteins, including muscle-specific kinase an additional target for antibodies. Later papers report the link between brain inflammation and severe amnesia, paraneoplastic and non-paraneoplastic, and the identification of the first p

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Alexander, D. J., N. Phin, and M. Zuckerman. Influenza. Edited by I. H. Brown. Oxford University Press, 2011. http://dx.doi.org/10.1093/med/9780198570028.003.0037.

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Influenza is a highly infectious, acute illness which has affected humans and animals since ancient times. Influenza viruses form the Orthomyxoviridae family and are grouped into types A, B, and C on the basis of the antigenic nature of the internal nucleocapsid or the matrix protein. Infl uenza A viruses infect a large variety of animal species, including humans, pigs, horses, sea mammals, and birds, occasionally producing devastating pandemics in humans, such as in 1918 when it has been estimated that between 50–100 million deaths occurred worldwide.There are two important viral surface glyc

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Baobaid, Mohammed, Lynda Ashbourne, Abdallah Badahdah, and Abir Al Jamal. Home / Publications / Pre and Post Migration Stressors and Marital Relations among Arab Refugee Families in Canada Pre and Post Migration Stressors and Marital Relations among Arab Refugee Families in Canada. 2nd ed. Hamad Bin Khalifa University Press, 2019. http://dx.doi.org/10.5339/difi_9789927137983.

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The study is funded by Doha International Family Institute (DIFI), a member of Qatar Foundation, and is a collaboration between the Muslim Resource Centre for Social Support and Integration of London, Ontario; University of Guelph, Ontario; and University of Calgary, Alberta, all located in Canada; and the Doha International Family Institute, Qatar. The study received research ethics approval from the University of Guelph and the University of Calgary. This study aims to assess the impact of pre- and post-migration on marital relationships and family dynamics for Arab refugee families resettle

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Book chapters on the topic "Related protein identification"

1

Doucet, Alain, and Daniel Figeys. "Identification of Chromatin-Binding Protein Complexes." In Systems Analysis of Chromatin-Related Protein Complexes in Cancer. Springer New York, 2013. http://dx.doi.org/10.1007/978-1-4614-7931-4_6.

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Atzeni, Paolo, Fabio Polticelli, and Daniele Toti. "An Automatic Identification and Resolution System for Protein-Related Abbreviations in Scientific Papers." In Evolutionary Computation, Machine Learning and Data Mining in Bioinformatics. Springer Berlin Heidelberg, 2011. http://dx.doi.org/10.1007/978-3-642-20389-3_18.

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Samah, Norshahida A., Azura Amid, and Faridah Yusof. "Identification of Aldolase C, Actin-Related Protein 2 Homolog, and Enolase 1 as Potential Biomarkers for Electrically Stunned Chickens." In Contemporary Issues and Development in the Global Halal Industry. Springer Singapore, 2016. http://dx.doi.org/10.1007/978-981-10-1452-9_47.

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Dong, Qunfeng, and Volker Brendel. "Computational Identification of Related Proteins." In The Proteomics Protocols Handbook. Humana Press, 2005. http://dx.doi.org/10.1007/978-1-59259-890-8_51.

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Weise, Christoph, Cassian Bon, and Xavier Cousin. "Identification of Residues Involved in Naja Naja Oxiana AChE Insensitivity to Fasciculin." In Structure and Function of Cholinesterases and Related Proteins. Springer US, 1998. http://dx.doi.org/10.1007/978-1-4899-1540-5_66.

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De Ferrari, Giancarlo V., and Nibaldo C. Inestrosa. "Identification of an Acetylcholinesterase Fragment that Promotes Alzheimer β-Amyloid Fibril Formation." In Structure and Function of Cholinesterases and Related Proteins. Springer US, 1998. http://dx.doi.org/10.1007/978-1-4899-1540-5_50.

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Collmer, Alan, B. H. Kvitko, J. E. Morello, K. R. Munkvold, H. S. Oh, and C. F. Wei. "Exploring the Functions of Proteins Secreted by the Hrp Type III Secretion System of Pseudomonas syringae." In Pseudomonas syringae Pathovars and Related Pathogens – Identification, Epidemiology and Genomics. Springer Netherlands, 2008. http://dx.doi.org/10.1007/978-1-4020-6901-7_24.

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Nouri, Mohammad-Zaman, Mohammad-Reza Ghaffari, Hamid Sobhanian, and Mohammad-Reza Hajirezaei. "Proteomics Approach for Identification of Nutrient Deficiency Related Proteins in Crop Plants." In Agricultural Proteomics Volume 2. Springer International Publishing, 2016. http://dx.doi.org/10.1007/978-3-319-43278-6_8.

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Plum, L., K. Giesen, R. Kluge, and H. G. Joost. "Identification of Susceptibility Loci for Obesity, Insulin Resistance, and Hyperglycemia in a Backcross Model of New Zealand Obese (NZO) and Lean SJL Mice." In Insulin & Related Proteins - Structure to Function and Pharmacology. Springer Netherlands, 2002. http://dx.doi.org/10.1007/0-306-47582-0_14.

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Palme, Klaus, Thomas Diefenthal, Chris Sander, Martin Vingron, and Jeff Schell. "Identification of Guanine-Nucleotide Binding Proteins in Plants: Structural Analysis and Evolutionary Comparison of the Ras-Related Ypt-Gene Family from Zea Mays." In The Guanine — Nucleotide Binding Proteins. Springer US, 1989. http://dx.doi.org/10.1007/978-1-4757-2037-2_27.

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Conference papers on the topic "Related protein identification"

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Tripathi, Rashmi, Pawan Sharma, Pavan Chakraborty, and Pritish Varadwaj. "In silico identification of novel virulent protein of phytophthora infestans related to late blight disease." In 2016 International Conference on Bioinformatics and Systems Biology (BSB). IEEE, 2016. http://dx.doi.org/10.1109/bsb.2016.7552145.

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Atzeni, Paolo, Fabio Polticelli, and Daniele Toti. "A framework for semi-automatic identification, disambiguation and storage of protein-related abbreviations in scientific literature." In 2011 IEEE International Conference on Data Engineering Workshops (ICDEW). IEEE, 2011. http://dx.doi.org/10.1109/icdew.2011.5767646.

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Felez, J., R. Rodriguez-Pinto, A. Oliver, et al. "MULTICENTRIC SPANISH STUDY OF BIOLOGICAL CAUSES OF DEEP VEIN THROMBOSIS." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643044.

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305 unselected patients under long-term oral anticoagulation treatment for having presented one o more ep^L sodes of deep vein thrombosis and/or pulmonary embolism, have been studied for the following anomalies: Dysfibrinogenemia, Lupus anticoagulant, Antithrombin-III deficiency, Protein C, Protein S, Heparin Cofactor II, and anomalies in the fibrinolytic components t-PA PAI and Plasminogen. Protein C antigen and activity as well as free Protein S antigen levels have been related to those found in a control group at different intensities of oral anticoagulantAs shown in the table this study, p

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Chung, Eunna, and Marissa Nichole Rylander. "Effects of Growth Factors and Stress Conditioning on the Induction of Heat Shock Proteins and Osteogenesis." In ASME 2009 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2009. http://dx.doi.org/10.1115/sbc2009-206662.

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Tissue engineering is an emerging field that focuses on development of methods for repairing and regenerating damaged or diseased tissue. Successful development of engineered tissues is often limited by insufficient cellular proliferation and insufficient formation of extracellular matrix. To induce effective bone regeneration, many research groups have investigated the cellular response and capability for tissue regeneration associated with bioreactor conditions and addition of growth factors [1]. Bioreactors in tissue engineering have been developed to expose cells to a similar stress enviro

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Abdel-Fatah, TMA, D. Agarwal, D. Zafeiris, et al. "Abstract P6-09-16: Identification of proliferation related derivers and their roles in precision medicine for breast cancers: A retrospective multidimensional comparative, integrated genomic, transcriptomic, and protein analysis." In Abstracts: 2016 San Antonio Breast Cancer Symposium; December 6-10, 2016; San Antonio, Texas. American Association for Cancer Research, 2017. http://dx.doi.org/10.1158/1538-7445.sabcs16-p6-09-16.

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Lipinska, I., C. Perlgrund, T. Wharton, and Y. Gurewich. "FIBRINOGEN HETEROGENEITY AND FIBRINOLYTIC ACTIVITY IN AN6I0GRAPHICALLY ASSESSED CORONARY ARTERY DISEASE (CAD)." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643027.

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Human plasma was shown previously to contain three forms of thrombin clottable protein: high molecular (HMW) and two lower molecular weight fibrinogen (LMW and LMW*) , the percentage of which in healthy subject is 69, 28 and 32 respectively. Significant abnormalities in the proportion of fibrinogen (Fgb) fractions were observed post–surgically, in cancer, and in patients with myocardial infarction and liver diseases. The aim of this study was to determine Fbg fractions and fibrinolytic activity (FA) in patients with and without proven coronary occlusion. Group I consisted of 21 symptomatic pat

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Vehar, G. A. "THE PRESENT STATE OF GENE TECHNOLOGY IN THE MANUFACTURE OF HUMAN COAGULATION PROTEINS." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644755.

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The production of pharmaceuticals from human plasma that are useful in the treatment of bleeding disorders had its beginning with the development of the Cohn fractionation procedure in the 1940's. As a result of these advances, concentrates became available for the treatment of the hemophilias. Although of low purity and subject to contamination by hepatitis virus, the availability of these compounds resulted in dramatic improvements in the life expectancy and quality of life of afflicted individuals. The numerous problems associated with production of pharmaceuticals from pooled plasma made t

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González-Rodríguez, L., V. Calamia, L. Lourido, et al. "AB0249 Identification of inflammatory- and immune disorder- related proteins as putative biomarker candidates for improving rheumatoid arthritis monitoring." In Annual European Congress of Rheumatology, 14–17 June, 2017. BMJ Publishing Group Ltd and European League Against Rheumatism, 2017. http://dx.doi.org/10.1136/annrheumdis-2017-eular.4912.

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Marquerie, G., A. Duperray, G. Uzan, and R. Berthier. "BIOSYNTHETIC PATHWAYS OF THE PLATELET FIBRINOGEN RECEPTOR IN HUMAN MEGAKARYOCYTES." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1642954.

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Interaction between cells and between cells and extracellular matrices are critical for a number of biological processes, including organ development, cell differenciation, cell motility, and the inimune' response. These interactions are mediated by a family of adhesion receptors that recognize short sequences such as Arg-Gly-Asp (RGD). These receptors share similar structural properties. They are heterodimers composed of a and B subunits and sometime express common epitopes. This suggests that the structural and functional relationship of these receptors may result from the transcription of r

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Grøn, B., and F. Brosstad. "IMMUNO-VISUALIZATION OF FIBRINOGEN Aa-CHAIN HETEROGENEITY IN NORMAL PLASMA COMPARED TO PLASMA FROM PATIENTS WITH DIC." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643325.

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Citrated plasma from healthy individuals or fran patients with disseminated intravascular coagulation(DIC) was diluted, and elec-trophoresed on 11% SDS-PAG slabs after reduction.Subsequently, the electrophoretic pattern was Western-blotted onto nitrocellulose and,-after blocking with gelatine-, reacted with either:a)polyclonal antibodies to human fibrinogen,b)polyclonal antibodies to FPA, or c)monoclonal antibody to non-released FPA (gift from Dr.Nieuwenhuizen, Leyden, Holland).Visualization of fibrinogen-related material was then done with peroxidase-conjugated secondary antibodies.In normal

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