Relevant bibliographies by topics / Relaxase

Contents

  1. Journal articles
  2. Dissertations / Theses
  3. Books
  4. Book chapters
  5. Conference papers
  6. Reports

Academic literature on the topic 'Relaxase'

Author: Grafiati
Published: 4 June 2021
Last updated: 8 June 2024

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Journal articles on the topic "Relaxase"

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Dostál, Lubomír, and Joel F. Schildbach. "Single-Stranded DNA Binding by F TraI Relaxase and Helicase Domains Is Coordinately Regulated." Journal of Bacteriology 192, no. 14 (2010): 3620–28. http://dx.doi.org/10.1128/jb.00154-10.

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ABSTRACT Transfer of conjugative plasmids requires relaxases, proteins that cleave one plasmid strand sequence specifically. The F plasmid relaxase TraI (1,756 amino acids) is also a highly processive DNA helicase. The TraI relaxase activity is located within the N-terminal ∼300 amino acids, while helicase motifs are located in the region comprising positions 990 to 1450. For efficient F transfer, the two activities must be physically linked. The two TraI activities are likely used in different stages of transfer; how the protein regulates the transition between activities is unknown. We exami
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Pérez-Mendoza, Daniel, María Lucas, Socorro Muñoz, et al. "The Relaxase of the Rhizobium etli Symbiotic Plasmid Shows nic Site cis-Acting Preference." Journal of Bacteriology 188, no. 21 (2006): 7488–99. http://dx.doi.org/10.1128/jb.00701-06.

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ABSTRACT Genetic and biochemical characterization of TraA, the relaxase of symbiotic plasmid pRetCFN42d from Rhizobium etli, is described. After purifying the relaxase domain (N265TraA), we demonstrated nic binding and cleavage activity in vitro and thus characterized for the first time the nick site (nic) of a plasmid in the family Rhizobiaceae. We studied the range of N265TraA relaxase specificity in vitro by testing different oligonucleotides in binding and nicking assays. In addition, the ability of pRetCFN42d to mobilize different Rhizobiaceae plasmid origins of transfer (oriT) was examin
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Jandle, Sarah, and Richard Meyer. "Stringent and Relaxed Recognition of oriT by Related Systems for Plasmid Mobilization: Implications for Horizontal Gene Transfer." Journal of Bacteriology 188, no. 2 (2006): 499–506. http://dx.doi.org/10.1128/jb.188.2.499-506.2006.

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ABSTRACT The plasmids R1162 and pSC101 have origins of conjugative transfer (oriTs) and corresponding relaxases that are closely related. The oriTs are made up of a highly conserved core, where DNA is cleaved by the relaxase prior to transfer, and an inverted repeat that differs in size and sequence. We show that in each case the seven base pairs adjacent to the core and within one arm of the inverted repeat are sufficient to determine specificity. Within this DNA there are three AT base pairs located 4 bp from the core. Mutations in the AT base pairs suggest that the relaxase makes essential
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Pluta, Radoslaw, D. Roeland Boer, Fabián Lorenzo-Díaz, et al. "Structural basis of a histidine-DNA nicking/joining mechanism for gene transfer and promiscuous spread of antibiotic resistance." Proceedings of the National Academy of Sciences 114, no. 32 (2017): E6526—E6535. http://dx.doi.org/10.1073/pnas.1702971114.

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Relaxases are metal-dependent nucleases that break and join DNA for the initiation and completion of conjugative bacterial gene transfer. Conjugation is the main process through which antibiotic resistance spreads among bacteria, with multidrug-resistant staphylococci and streptococci infections posing major threats to human health. The MOBV family of relaxases accounts for approximately 85% of all relaxases found in Staphylococcus aureus isolates. Here, we present six structures of the MOBV relaxase MobM from the promiscuous plasmid pMV158 in complex with several origin of transfer DNA fragme
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Libante, Virginie, Nazim Sarica, Abbas Mohamad Ali, et al. "Mobilization of IMEs Integrated in the oriT of ICEs Involves Their Own Relaxase Belonging to the Rep-Trans Family of Proteins." Genes 11, no. 9 (2020): 1004. http://dx.doi.org/10.3390/genes11091004.

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Integrative mobilizable elements (IMEs) are widespread but very poorly studied integrated elements that can excise and hijack the transfer apparatus of co-resident conjugative elements to promote their own spreading. Sixty-four putative IMEs, harboring closely related mobilization and recombination modules, were found in 14 Streptococcus species and in Staphylococcus aureus. Fifty-three are integrated into the origin of transfer (oriT) of a host integrative conjugative element (ICE), encoding a MobT relaxase and belonging to three distant families: ICESt3, Tn916, and ICE6013. The others are in
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Heilers, Jan-Hendrik, Jens Reiners, Eva-Maria Heller, Annika Golzer, Sander H. J. Smits, and Chris van der Does. "DNA processing by the MOBH family relaxase TraI encoded within the gonococcal genetic island." Nucleic Acids Research 47, no. 15 (2019): 8136–53. http://dx.doi.org/10.1093/nar/gkz577.

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Abstract Relaxases of the MOBH family are often found on large plasmids, genetic islands and integrative conjugative elements. Many members of this family contain an N-terminal relaxase domain (TraI_2) followed by a disordered middle part and a C-terminal domain of unknown function (TraI_2_C). The TraI_2 domain contains two putative metal-binding motifs, an HD domain motif and an alternative 3H motif. TraI, encoded within the gonococcal genetic island of Neisseria gonorrhoeae, is the prototype of the MOBH family. SAXS experiments showed that TraI_2 and TraI_2_C form globular structures separat
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Haft, Rembrandt J. F., Gilberto Palacios, Tran Nguyen, et al. "General Mutagenesis of F Plasmid TraI Reveals Its Role in Conjugative Regulation." Journal of Bacteriology 188, no. 17 (2006): 6346–53. http://dx.doi.org/10.1128/jb.00462-06.

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ABSTRACT Bacteria commonly exchange genetic information by the horizontal transfer of conjugative plasmids. In gram-negative conjugation, a relaxase enzyme is absolutely required to prepare plasmid DNA for transit into the recipient via a type IV secretion system. Here we report a mutagenesis of the F plasmid relaxase gene traI using in-frame, 31-codon insertions. Phenotypic analysis of our mutant library revealed that several mutant proteins are functional in conjugation, highlighting regions of TraI that can tolerate insertions of a moderate size. We also demonstrate that wild-type TraI, whe
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Staddon, Jack H., Edward M. Bryan, Dawn A. Manias, and Gary M. Dunny. "Conserved Target for Group II Intron Insertion in Relaxase Genes of Conjugative Elements of Gram-Positive Bacteria." Journal of Bacteriology 186, no. 8 (2004): 2393–401. http://dx.doi.org/10.1128/jb.186.8.2393-2401.2004.

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ABSTRACT The lactococcal group II intron Ll.ltrB interrupts the ltrB relaxase gene within a region that encodes a conserved functional domain. Nucleotides essential for the homing of Ll.ltrB into an intronless version of ltrB are found exclusively at positions required to encode amino acids broadly conserved in a family of relaxase proteins of gram-positive bacteria. Two of these relaxase genes, pcfG from the enterococcal plasmid pCF10 and the ORF4 gene in the streptococcal conjugative transposon Tn5252, were shown to support Ll.ltrB insertion into the conserved motif at precisely the site pre
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Meyer, Richard. "Mapping Type IV Secretion Signals on the Primase Encoded by the Broad-Host-Range Plasmid R1162 (RSF1010)." Journal of Bacteriology 197, no. 20 (2015): 3245–54. http://dx.doi.org/10.1128/jb.00443-15.

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ABSTRACTThe plasmid R1162 (RSF1010) encodes a primase essential for its replication. This primase makes up the C-terminal part of MobA, a multifunctional protein with the relaxase as a separate N-terminal domain. The primase is also translated separately as the protein RepB′. Here, we map two signals for type IV secretion onto the recently solved structure of RepB′. One signal is located internally within RepB′ and consists of a long α-helix and an adjacent disordered region rich in arginines. The second signal is made up of the same α-helix and a second, arginine-rich region at the C-terminal
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Breidenstein, Annika, Josy ter Beek, and Ronnie P.-A. Berntsson. "Structural and functional characterization of TraI from pKM101 reveals basis for DNA processing." Life Science Alliance 6, no. 4 (2023): e202201775. http://dx.doi.org/10.26508/lsa.202201775.

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Type 4 secretion systems are large and versatile protein machineries that facilitate the spread of antibiotic resistance and other virulence factors via horizontal gene transfer. Conjugative type 4 secretion systems depend on relaxases to process the DNA in preparation for transport. TraI from the well-studied conjugative plasmid pKM101 is one such relaxase. Here, we report the crystal structure of the trans-esterase domain of TraI in complex with its substrateoriTDNA, highlighting the conserved DNA-binding mechanism of conjugative relaxases. In addition, we present an apo structure of the tra
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Dissertations / Theses on the topic "Relaxase"

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Pluta, Radoslaw 1984. "Structural basis of conjugative DNA transfer mediated by MobM, a prototype of the major relaxase family of Staphylococcus aureus." Doctoral thesis, Universitat Pompeu Fabra, 2014. http://hdl.handle.net/10803/346933.

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MobM relaxase from the promiscuous antibiotic resistance plasmid pMV158 is a prototype of the Mob_Pre/MOBV family of relaxases, the major family of relaxases found in Staphylococcus aureus. Staphylococcal infections cause the highest number of lethal cases among antibiotic-resistant bacterial infections. Relaxases initiate the conjugative DNA transfer, a major route for the antibiotic resistance acquisition in bacteria, by nicking their substrate DNA through formation of a covalent DNA-relaxase adduct and terminate the transfer in the recipient cells by rejoining ends of the linearized plasmi
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Stern, Jennifer C. "Single-stranded DNA recognition by F factor TraI and the TraI relaxase domain." Available to US Hopkins community, 2002. http://wwwlib.umi.com/dissertations/dlnow/3068216.

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Laroussi, Haifa. "Étude des mécanismes moléculaires d'initiation du transfert conjugatif d'ICESt3, médiée par une relaxase MOBT chez la bactérie Gram+ Streptococcus thermophilus." Electronic Thesis or Diss., Université de Lorraine, 2022. http://www.theses.fr/2022LORR0176.

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Les génomes bactériens évoluent principalement grâce au transfert horizontal de gènes. La conjugaison bactérienne en est un des mécanismes majeurs. Elle est notamment médiée par les éléments intégratifs et conjugatifs (ICE). En plus de leur transfert, les ICE codent d'autres fonctions conférant à leur hôte un avantage adaptatif, comme par exemple des résistances aux antibiotiques dont la diffusion est un enjeu majeur en santé publique. Il est donc nécessaire de comprendre comment les ICE se transfèrent si l'on veut limiter leur dissémination. Le transfert d'un ICE d'une cellule donatrice vers
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Kopec, Jolanta. "Structure analysis of DNA relaxases, the key enzymes of bacterial conjugation traA and its N-terminal relaxase domain of the Gram-positive plasmid pIP501 show specific oriT binding and behave as dimers in solution /." [S.l.] : [s.n.], 2006. http://deposit.ddb.de/cgi-bin/dokserv?idn=981331483.

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Kennedy, Sarah A. Redinbo Matthew Robert. "Structural and molecular dynamics simulation studies support Symplekin's protein scaffolding role and a novel fold in the TraI relaxase-helicase C-terminus is essential for conjugative DNA transfer." Chapel Hill, N.C. : University of North Carolina at Chapel Hill, 2009. http://dc.lib.unc.edu/u?/etd,2343.

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Thesis (Ph. D.)--University of North Carolina at Chapel Hill, 2009.<br>Title from electronic title page (viewed Jun. 26, 2009). "... in partial fulfillment of the requirements for the degree of Doctor of Philosophy in the Department of Chemistry Biological Chemistry. " Discipline: Chemistry; Department/School: Chemistry.
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Heller, Eva Maria [Verfasser], and Lotte [Akademischer Betreuer] Sogaard-Andersen. "Biochemical characterization of the relaxase TraI, the coupling protein TraD and the hypothetical protein Yaf of the novel Type IV Secretion System from the human pathogen Neisseria gonorrhoeae / Eva-Maria Heller. Betreuer: Lotte Sogaard-Andersen." Marburg : Philipps-Universität Marburg, 2014. http://d-nb.info/105193463X/34.

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Frösslová, Markéta. "Determinanty herecké práce? relaxace a reflexe." Master's thesis, Akademie múzických umění v Praze.Divadelní fakulta. Knihovna, 2012. http://www.nusl.cz/ntk/nusl-156050.

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The Thesis describes the determinants of acting that I experienced during my studies at DAMU and in my following career. Specially is described the director?s character as one as a main determinant which affects the actor?s progression. The communication as a general determinant is set to be the one which influences the cooperation between actor and director the most. Own selfreflexion is treated as well.
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Musil, Vladimír. "Relaxace smykového napětí asfaltových pojiv v DSR." Master's thesis, Vysoké učení technické v Brně. Fakulta stavební, 2015. http://www.nusl.cz/ntk/nusl-227690.

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The thesis describes the relaxation properties of bituminous binders determined in dynamic shear rheometer (DSR). Paving bitumen, polymer modified bitumen (PMB) and crumb rubber modified bitumen (CRmB) were chosen for comparison. In order to describe the effects of ageing on asphalt binders relaxation properties, the laboratory simulation of ageing using RTFOT and 3xRTFOT method was performed. For each binder were simultaneously conducted tests of needle penetration, softening point and elastic recovery. Selected bitumens were used for the production of asphalt mixtures. The low-temperature pr
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Doupal, Ondřej. "Zařízení pro sport a relaxaci." Master's thesis, Vysoké učení technické v Brně. Fakulta stavební, 2013. http://www.nusl.cz/ntk/nusl-225761.

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The project design processes multifunctional center for leisure and sports. The center is situated in Šternberk and its capacity is about 150 people. There is inter alia the wellness center, with saunas and whirlpool, a fitness, gym and a beauty salon. Architecturally, the object is conceived as paralell blending of three units of a different colors and sizes. As well as all construction compositions are more developed basis for air conditioning systems. Documentation is processed at the level of documentation for building permit.
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PONTREMOLI, GUIDO. "New Muscle Relaxant Glycosylthiocolchicine derivatives." Doctoral thesis, Università degli Studi di Milano, 2005. http://hdl.handle.net/2434/153952.

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A novel class of 3-demethoxy-3-glycosylaminothiocolchicines (7) was prepared and tested for muscle relaxant activity. The syntheses were performed starting from the new 3-amino-3-demethoxythiocolchicine (5) prepared in good yield from 3-O-demethylthiocolchicine (1c) using the Buchwald-Hartwig reaction. The condensation of 5 with a series of pentose and hexose sugars (6) gave a series of 3-demethoxy-3-glycosylaminothiocolchicines (7). Their preparation was accomplished by adapting and improving a previous procedure for the preparation of N-arylglycosylamines. In particular, replacing tradi
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Books on the topic "Relaxase"

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Șain, Emil. Relaxare. Editura Eminescu, 1986.

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Nissanoff, Jonathan. Curare: muscle relaxant. Rand Corp., 1988.

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Philippe, Decaluwé, ed. Mindfulness voor je kids: Oefeningen. Lannoo, 2011.

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Le massage: Tonifiant, relaxant, antistress. De Vecchi, 1997.

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Walji, Hasnain. Kava: Nature's relaxant for anxiety, stress, and pain. Hohm Press, 1997.

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Linkens, D. A. Self-organising learning control and its applications to muscle relaxant anaesthesia. University of Sheffield, Dept. of Control Engineering, 1990.

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Linkens, D. A. Self-organising fuzzy logic control and its application to muscle relaxant anaesthesia. University of Sheffield, Dept. of Control Engineering, 1990.

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Halpern, Céline. Médecins: Relaxe ou condamnation : tout savoir sur les contentieux médicaux. Milo, 2006.

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Kava Kava: The anti-anxiety herb that relaxes and sharpens the mind. Keats Pub., 1997.

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Elisa, Duijvenstein, and Boon Debbie, eds. Chill: Toptips voor relaxte meiden : test jezelf, maak lekkere snacks en doe iets creatiefs. Kluitman, 2006.

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Book chapters on the topic "Relaxase"

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Lucas, María, and Gabriel Moncalián. "Identification of Relaxase-DNA Covalent Complexes and DNA Strand Transfer Reaction Products by Polyacrylamide Gel Electrophoresis." In Horizontal Gene Transfer. Springer US, 2019. http://dx.doi.org/10.1007/978-1-4939-9877-7_11.

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Cuartas, Raquel, Teresa M. Coque, Fernando de la Cruz, and M. Pilar Garcillán-Barcia. "PLASmid TAXonomic PCR (PlasTax-PCR), a Multiplex Relaxase MOB Typing to Assort Plasmids into Taxonomic Units." In Methods in Molecular Biology. Springer US, 2021. http://dx.doi.org/10.1007/978-1-0716-1799-1_10.

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Beer, Renée, and Ramón Lindauer. "Relaxatie." In Verwerken en versterken. Bohn Stafleu van Loghum, 2014. http://dx.doi.org/10.1007/978-90-368-0582-7_2.

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Beer, Renée, and Ramón Lindauer. "Relaxatie." In Verwerken en versterken. Bohn Stafleu van Loghum, 2014. http://dx.doi.org/10.1007/978-90-368-0584-1_2.

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Bährle-Rapp, Marina. "relaxant." In Springer Lexikon Kosmetik und Körperpflege. Springer Berlin Heidelberg, 2007. http://dx.doi.org/10.1007/978-3-540-71095-0_8837.

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Garcillán-Barcia, M. Pilar, Santiago Redondo-Salvo, Luis Vielva, and Fernando de la Cruz. "MOBscan: Automated Annotation of MOB Relaxases." In Horizontal Gene Transfer. Springer US, 2019. http://dx.doi.org/10.1007/978-1-4939-9877-7_21.

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Kardener, Sheldon H. "Hypnosis — The “Natural” Relaxant." In Psychiatry the State of the Art. Springer US, 1985. http://dx.doi.org/10.1007/978-1-4684-4697-5_47.

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Sampankanpanich Soria MD, Claire, Daniel E. Lee MD, PhD, and Gerard R. Manecke MD. "Intubating Without Muscle Relaxant." In Anesthesiology Resident Manual of Procedures. Springer International Publishing, 2021. http://dx.doi.org/10.1007/978-3-030-65732-1_11.

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Hollingsworth, Michael, Sarah Rudkin, and Sandra Downing. "Myometrial relaxant action of relaxin." In Relaxin 2000. Springer Netherlands, 2001. http://dx.doi.org/10.1007/978-94-017-2877-5_49.

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Zechner, Ellen L., Gabriel Moncalián, and Fernando de la Cruz. "Relaxases and Plasmid Transfer in Gram-Negative Bacteria." In Current Topics in Microbiology and Immunology. Springer International Publishing, 2017. http://dx.doi.org/10.1007/978-3-319-75241-9_4.

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Conference papers on the topic "Relaxase"

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Silva, Vaneza Gomes da, and Paula Gomes Barroso. "VENTOSATERAPIA: UM INSTRUMENTO POTENTE DE VINCULAÇÃO E CUIDADO EM SAÚDE EM UMA COMUNIDADE EM FORTALEZA." In I Congresso Brasileiro de Saúde Pública On-line: Uma abordagem Multiprofissional. Revista Multidisciplinar em Saúde, 2021. http://dx.doi.org/10.51161/rems/2771.

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Introdução: Com o período de pandemia vivenciado em 2020 e 2021, causado pelo vírus SARS – Cov 2, foi percebido um grande impacto na vida cotidiana da população devido ao isolamento social, a sobrecarga emocional e as dificuldades socioeconômicas que têm enfraquecido os vínculos em relação à população e a saúde. Toda essa situação foi geradora de aflições, medo, tensões musculares, dores, alterações físicas e até mesmo psíquicas. A Ventosaterapia é uma técnica originária milenar da Medicina Tradicional Chinesa (MTC) que utiliza copos que produzem vácuo nas estruturas musculares, com objetivo d
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Al-Jumaily, Ahmed, and Meha Mathur. "Improving relaxant effect of contracted airway smooth muscles." In 2014 Middle East Conference on Biomedical Engineering (MECBME). IEEE, 2014. http://dx.doi.org/10.1109/mecbme.2014.6783210.

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Chen, Po-Chun, Peng Liu, John Yen, and Tracy Mullen. "Experience-based cyber situation recognition using relaxable logic patterns." In 2012 IEEE International Multi-Disciplinary Conference on Cognitive Methods in Situation Awareness and Decision Support (CogSIMA 2012). IEEE, 2012. http://dx.doi.org/10.1109/cogsima.2012.6188392.

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Mahfouf, M., and D. A. Linkens. "A nonlinear GPC algorithm (NLGPC) for muscle relaxant anaesthesia." In 1997 European Control Conference (ECC). IEEE, 1997. http://dx.doi.org/10.23919/ecc.1997.7081899.

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Sheu, Ruey-Kai, Win-Tsung Lo, Chia-Feng Lin, and Shyan-Ming Yuan. "Design and Implementation of a Relaxable Web Service Composition System." In 2010 International Conference on Cyber-Enabled Distributed Computing and Knowledge Discovery (CyberC). IEEE, 2010. http://dx.doi.org/10.1109/cyberc.2010.89.

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Martin, Christian, Julia Krabbe, Stefan Uhlig, et al. "Nintedanib relaxes human pulmonary arteries more potently than murine." In ERS International Congress 2017 abstracts. European Respiratory Society, 2017. http://dx.doi.org/10.1183/1393003.congress-2017.pa2374.

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Mason, D. G. "Hybrid self-organising fuzzy logic PID controllers for muscle relaxant anaesthesia." In UKACC International Conference on Control. Control '96. IEE, 1996. http://dx.doi.org/10.1049/cp:19960615.

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Cafure, Patrícia Dias Neder, and Soraya Chicrala Matos. "MASSAGEM RELAXANTE ASSOCIADA A AROMATERAPIA PARA AUXILIAR NO COMBATE DA ANSIEDADE." In Anais do 2º CONIGRAN - Congresso Integrado Unigran Capital. Even3, 2021. http://dx.doi.org/10.29327/144454.2-3.

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Cafure, Patrícia Dias Neder, and Soraya Chicrala Matos. "MASSAGEM RELAXANTE ASSOCIADA A AROMATERAPIA PARA AUXILIAR NO COMBATE DA ANSIEDADE." In Anais do 2º CONIGRAN - Congresso Integrado Unigran Capital. Even3, 2021. http://dx.doi.org/10.29327/144454.2-3.

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Lombard, A. I., D. Villalba, A. P. Nayak, and R. B. Penn. "Chronic Lorazepam-Induced Pro-Relaxant Signaling in ASM Cells Is Cyclooxygenase Dependent." In American Thoracic Society 2020 International Conference, May 15-20, 2020 - Philadelphia, PA. American Thoracic Society, 2020. http://dx.doi.org/10.1164/ajrccm-conference.2020.201.1_meetingabstracts.a1251.

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Reports on the topic "Relaxase"

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Bernal, CArolina, and Razvan Vlaicu. Child Labor, Rainfall Shocks, and Financial Inclusion: Evidence from Rural Households. Inter-American Development Bank, 2023. http://dx.doi.org/10.18235/0005058.

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This paper examines how rural households cope with climate change related rainfall shocks by re-allocating childrens time between domestic activities and school attendance. Households affected by an unanticipated rainfall shock face an inter-temporal trade-off between current household income and future potential earnings. Financial inclusion may mitigate or exacerbate the human capital impacts of rainfall shocks depending on whether it relaxes or constrains household budgets. The data come from a three-round panel household survey in rural Colombia collected between 2010-2016. The main findin
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