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Journal articles on the topic 'Repeat construction'

Author: Grafiati

Published: 3 June 2025

Last updated: 31 July 2025

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1

Rudenko, Valentina, and Eugene Korotkov. "Study of Dispersed Repeats in the Cyanidioschyzon merolae Genome." International Journal of Molecular Sciences 25, no. 8 (2024): 4441. http://dx.doi.org/10.3390/ijms25084441.

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In this study, we applied the iterative procedure (IP) method to search for families of highly diverged dispersed repeats in the genome of Cyanidioschyzon merolae, which contains over 16 million bases. The algorithm included the construction of position weight matrices (PWMs) for repeat families and the identification of more dispersed repeats based on the PWMs using dynamic programming. The results showed that the C. merolae genome contained 20 repeat families comprising a total of 33,938 dispersed repeats, which is significantly more than has been previously found using other methods. The re

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2

Dunn and Anderson. "To Repeat or Not to Repeat: Repetitive Sequences Regulate Genome Stability in Candida albicans." Genes 10, no. 11 (2019): 866. http://dx.doi.org/10.3390/genes10110866.

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Genome instability often leads to cell death but can also give rise to innovative genotypic and phenotypic variation through mutation and structural rearrangements. Repetitive sequences and chromatin architecture in particular are critical modulators of recombination and mutability. In Candida albicans, four major classes of repeats exist in the genome: telomeres, subtelomeres, the major repeat sequence (MRS), and the ribosomal DNA (rDNA) locus. Characterization of these loci has revealed how their structure contributes to recombination and either promotes or restricts sequence evolution. The

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3

Swennenhuis, Joost F., Brad Foulk, Frank A. W. Coumans, and Leon W. M. M. Terstappen. "Construction of repeat-free fluorescence in situ hybridization probes." Nucleic Acids Research 40, no. 3 (2011): e20-e20. http://dx.doi.org/10.1093/nar/gkr1123.

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4

Tozaki, T., S. Inoue, S. Mashima, M. Ohta, N. Miura, and M. Tomita. "Sequence analysis of trinucleotide repeat microsatellites from an enrichment library of the equine genome." Genome 43, no. 2 (2000): 354–65. http://dx.doi.org/10.1139/g99-140.

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Microsatellites are useful tools for the construction of a linkage map and parentage testing of equines, but only a limited number of equine microsatellites have been elucidated. Thus, we constructed the equine genomic library enriched for DNA fragments containing (CAG)n repeats. The enriched method includes hybridization-capture of repeat regions using biotin-conjugated oligonucleotides, nucleotide substrate-biased polymerase reaction with the oligonucleotides and subsequent PCR amplification, because these procedures are useful for the cloning of less abundant trinucleotide microsatellites.

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Wang, Zengli, and Hong Zhang. "Construction, Detection, and Interpretation of Crime Patterns over Space and Time." ISPRS International Journal of Geo-Information 9, no. 6 (2020): 339. http://dx.doi.org/10.3390/ijgi9060339.

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Empirical studies have focused on investigating the interactive relationships between crime pairs. However, many other types of crime patterns have not been extensively investigated. In this paper, we introduce three basic crime patterns in four combinations. Based on graph theory, the subgraphs for each pattern were constructed and analyzed using criminology theories. A Monte Carlo simulation was conducted to examine the significance of these patterns. Crime patterns were statistically significant and generated different levels of crime risk. Compared to the classical patterns, combined patte

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6

Gritsun, T. S., and E. A. Gould. "The 3′ untranslated regions of Kamiti River virus and Cell fusing agent virus originated by self-duplication." Journal of General Virology 87, no. 9 (2006): 2615–19. http://dx.doi.org/10.1099/vir.0.81950-0.

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Previously, it was shown that the 3′ untranslated region (3′UTR) of Kamiti River virus (KRV) is nearly twice as long as the 3′UTR of other flaviviruses (1208 nucleotides compared with 730 nucleotides for the longest 3′UTR of any virus in the Tick-borne encephalitis virus species). Additionally, KRV and the closely related Cell fusing agent virus (CFAV) were shown to contain two short, almost perfect repeat sequences of 67 nucleotides. However, the construction of a robust comparative nucleotide alignment has now revealed that the double-length 3′UTR and the direct repeats resulted from the vir

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7

Liu, Bingjian, Luxiu Gao, Yifan Liu, et al. "The First Genome-Wide Survey Analysis of the Tibetan Plateau Tetraploid Schizothorax curvilabiatus Reveals Its Microsatellite Characteristics and Phylogenetic Relationships." Genes 16, no. 5 (2025): 491. https://doi.org/10.3390/genes16050491.

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Background/Objectives: Schizothorax curvilabiatus, a typical highland polyploid species within the subfamily Schizothoracinae, holds economic value and ecological research significance. Currently, there are no related genomic studies. To obtain its genetic information and lay the foundation for subsequent whole-genome map construction, this study conducted a genome survey analysis, preliminary genome assembly, microsatellite identification, repeat sequence annotation, mitochondrial genome characterization, and phylogenetic relationship research. Methods: DNA was sequenced on a DNBSEQ-T7 platfo

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Fedorova, Olga V. "Syntactic priming in experimental linguistics." Rhema, no. 2, 2020 (2020): 92–120. http://dx.doi.org/10.31862/2500-2953-2020-2-92-120.

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This paper describes the phenomenon of syntactic priming, that is, the speaker’s tendency to repeat the syntactic construction of an utterence used by himself or his interlocutor shortly before. The main theoretical concepts, experimental methodology, syntactic material, the so-called lexical boost effect, differences in results when using speech production or comprehension methods are considered, as well as the main theoretical models of syntactic priming. Experiments with bilinguals are described in detail. The results of the study of Russian possessive constructions are presented.

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9

Hughes, Spencer A., Fengbin Wang, Shengyuan Wang, et al. "Ambidextrous helical nanotubes from self-assembly of designed helical hairpin motifs." Proceedings of the National Academy of Sciences 116, no. 29 (2019): 14456–64. http://dx.doi.org/10.1073/pnas.1903910116.

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Tandem repeat proteins exhibit native designability and represent potentially useful scaffolds for the construction of synthetic biomimetic assemblies. We have designed 2 synthetic peptides, HEAT_R1 and LRV_M3Δ1, based on the consensus sequences of single repeats of thermophilic HEAT (PBS_HEAT) and Leucine-Rich Variant (LRV) structural motifs, respectively. Self-assembly of the peptides afforded high-aspect ratio helical nanotubes. Cryo-electron microscopy with direct electron detection was employed to analyze the structures of the solvated filaments. The 3D reconstructions from the cryo-EM ma

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10

Alani, Eric, Liang Cao, and Nancy Kleckner. "A Method for Gene Disruption That Allows Repeated Use of URA3 Selection in the Construction of Multiply Disrupted Yeast Strains." Genetics 116, no. 4 (1987): 541–45. http://dx.doi.org/10.1093/genetics/116.4.541.

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Abstract In this paper, we describe a 3.8-kb molecular construct that we have used to disrupt yeast genes. The construct consists of a functional yeast URA3 gene flanked by 1.1-kb direct repeats of a bacterial sequence. It is straightforward to insert the 3.8-kb segment into a cloned target gene of interest and then introduce the resulting disruption into the yeast genome by integrative transformation. An appropriate DNA fragment containing the disruption plus flanking homology can be obtained by restriction enzyme digestion. After introducing such fragments into yeast by transformation, stabl

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11

Dong, Li, Yuhan Sun, Keqi Zhao, et al. "Development and Application of EST-SSR Markers for DNA Fingerprinting and Genetic Diversity Analysis of the Main Cultivars of Black Locust (Robinia pseudoacacia L.) in China." Forests 10, no. 8 (2019): 644. http://dx.doi.org/10.3390/f10080644.

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Black locust (Robinia pseudoacacia L.) is an economically and ecologically important tree species which is used for pillar construction, honey production and soil improvement. More EST-SSR (Expressed sequence tag simple sequence repeat) markers of black locust can be used as a complement and improvement of Genomic-SSR markers for the identification of the function of gene and the construction of genetic map. Additionally, currently there is no simple method for identifying black locust cultivars. In this study, we obtained 2702 unigenes from 3095 expressed sequence tags (ESTs) from the Nationa

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12

Lyu, Qian. "The Construction of a Model for Predicting Users’ Repeat Purchase Behavior and its Impact on the Economic Efficiency of Enterprises." WSEAS TRANSACTIONS ON COMPUTER RESEARCH 11 (September 21, 2023): 303–15. http://dx.doi.org/10.37394/232018.2023.11.28.

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Aiming at the shortcomings in efficiency and accuracy of the current prediction methods of user repeat purchase behavior in e-commerce enterprises, an intelligent prediction model of user repeat purchase behavior based on machine learning was proposed. In order to enhance the quality of the experimental data, Kernel Principal Components Analysis (KPCA) and the synthetic Minority oversampling technique (SMOTE) were first used to preprocess the data. After that, repeat purchase behavior is predicted using a Support Vector Machine (SVM). Then, the Sparrow Search Algorithm (SSA), based on multi-st

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13

Liva, Gianluigi, Paola Pulini, and Marco Chiani. "On-Line Construction of Irregular Repeat Accumulate Codes for Packet Erasure Channels." IEEE Transactions on Wireless Communications 12, no. 2 (2013): 680–89. http://dx.doi.org/10.1109/twc.2012.121412120053.

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14

Puspa, Dewi, Yossyafra Yossyafra, and Yosritzal Yosritzal. "ANALISIS FAKTOR PENYEBAB REWORK PADA PEKERJAAN KONTRUKSI JALAN KOTA PADANG." Rang Teknik Journal 5, no. 2 (2022): 331–35. http://dx.doi.org/10.31869/rtj.v5i2.3125.

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Rework is a repeat job that is caused by an error from a construction project. In a construction activity almost always happens rework only the quantity is different. The purpose of this study is identify the factors that cause rework, identify the conditions of rework, identify the intensity of rework on road construction work item in the city of Padang. This study uses qualitative methods with a descriptive approach. The result of the study are the main factors that led to road construction rework in Padang city were design and documentation, managerial factor and resources. Little rework co

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15

Simankina, Tatiana, and Olga Sergeenkova. "Recourse Optimization of Construction on a Basis of the Frontal and Rhythmical Stream." Applied Mechanics and Materials 725-726 (January 2015): 984–89. http://dx.doi.org/10.4028/www.scientific.net/amm.725-726.984.

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In article is offered the method of calculation of a frontal and rhythmical stream, at which initial temporary operating conditions of crews periodically repeat (under a condition of continuous use of resources). It allows to provide the demanded duration of performance of work and the minimum quantity of resources.

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16

Zhao, Jiuli, Xiaowen Xu, and Lei Shan. "Use of a Space Graft in Rhinoplasty." Journal of Craniofacial Surgery 35, no. 2 (2023): 639–42. http://dx.doi.org/10.1097/scs.0000000000009850.

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Background: Construction of the nasal tip strut is an important part of rhinoplasty; the incidence of postoperative complications is closely related to the type of graft and the approach to cartilaginous framework construction. Objectives: To introduce a supplementary graft to support the cartilaginous framework. Methods: Forty patients (37 female and 3 male), aged from 18 to 40 years (average 29), received a rhinoplasty using a spacer graft. Of these, 12 were primary cases and the other 28 had a previous rhinoplasty procedure. Postoperatively, all patients were photographed and asked to compl

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17

Wright, C. F., and R. S. Zitomer. "Point mutations implicate repeated sequences as essential elements of the CYC7 negative upstream site in Saccharomyces cerevisiae." Molecular and Cellular Biology 5, no. 11 (1985): 2951–58. http://dx.doi.org/10.1128/mcb.5.11.2951-2958.1985.

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The transcription of the CYC7 gene of Saccharomyces cerevisiae, encoding the iso-2-cytochrome c protein, is controlled by two upstream regulatory elements, a positive element and a negative element. The nature of the DNA sequences in the negative element were investigated in a two-part approach. The first involved the construction of a CYC7-galK fusion gene which placed the coding sequence of the Escherichia coli galactokinase gene under the regulation of the CYC7 upstream sequences. This fusion allowed the quantitation by galactokinase enzyme assays of the effects on gene expression of a vari

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18

Wright, C. F., and R. S. Zitomer. "Point mutations implicate repeated sequences as essential elements of the CYC7 negative upstream site in Saccharomyces cerevisiae." Molecular and Cellular Biology 5, no. 11 (1985): 2951–58. http://dx.doi.org/10.1128/mcb.5.11.2951.

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The transcription of the CYC7 gene of Saccharomyces cerevisiae, encoding the iso-2-cytochrome c protein, is controlled by two upstream regulatory elements, a positive element and a negative element. The nature of the DNA sequences in the negative element were investigated in a two-part approach. The first involved the construction of a CYC7-galK fusion gene which placed the coding sequence of the Escherichia coli galactokinase gene under the regulation of the CYC7 upstream sequences. This fusion allowed the quantitation by galactokinase enzyme assays of the effects on gene expression of a vari

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19

Diwan, Noa, Arvind A. Bhagwat, Gary B. Bauchan, and Perry B. Cregan. "Simple sequence repeat DNA markers in alfalfa and perennial and annual Medicago species." Genome 40, no. 6 (1997): 887–95. http://dx.doi.org/10.1139/g97-115.

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Simple sequence repeat (SSR) or microsatellite DNA markers have been shown to function well in plant and mammalian species for genetic map construction and genotype identification. The objectives of the work reported here were to search GenBank for the presence of SSR-containing sequences from the genus Medicago, to assess the presence and frequency of SSR DNA in the alfalfa (Medicago sativa (L.) L. &L.) genome, and to examine the function of selected markers in a spectrum of perennial and annual Medicago species. The screening of an alfalfa genomic DNA library and sequencing of clones put

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20

McCabe, Brenda, and Simaan M. AbouRizk. "Performance measurement indices for simulated construction operations." Canadian Journal of Civil Engineering 28, no. 3 (2001): 383–93. http://dx.doi.org/10.1139/l01-004.

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This paper describes the development of indices useful in automating the experimentation process of a computer simulation. Simulation methodologies have been developed to model construction systems, but most of these systems require the experimentation process to be carried out manually. In achieving optimum performance, one has to repeat an exhaustive number of experiments. The indices can be used to automate this process, as they are indicators of bottlenecks in the system that can be tracked through the simulation output. They are based upon user-defined performance guidelines for the resou

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21

Valerio-Lepiniec, Marie, Agathe Urvoas, Anne Chevrel та ін. "The αRep artificial repeat protein scaffold: a new tool for crystallization and live cell applications". Biochemical Society Transactions 43, № 5 (2015): 819–24. http://dx.doi.org/10.1042/bst20150075.

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We have designed a new family of artificial proteins, named αRep, based on HEAT (acronym for Huntingtin, elongation factor 3 (EF3), protein pphosphatase 2A (PP2A), yeast kinase Tor1) repeat proteins containing an α-helical repeated motif. The sequence of the repeated motifs, first identified in a thermostable archae protein was optimized using a consensus design strategy and used for the construction of a library of artificial proteins. All proteins from this library share the same general fold but differ both in the number of repeats and in five highly randomized amino acid positions within e

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22

Fraga Vidal, Reinaldo, Roberto Carlos Arísticas Ribalta, Lisandra Teresa Martínez Valdés, et al. "Construction of a Novel Chimeric Dextransucrase Fused to the Carbohydrate-Binding Module CBM2a." Catalysts 11, no. 10 (2021): 1179. http://dx.doi.org/10.3390/catal11101179.

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Lactic acid bacteria (LAB) have the potential to produce homoexopolysaccharides (HoPS). Their health benefits and physicochemical properties have been the subject of extensive research. The HoPS functional properties are determined by molecular weight, the type of glycosidic linkages, degrees of branching and chemical composition. The dextransucrases (DSases) produce a kind of HoPS (dextrans), which are among the first biopolymers produced at industrial scale with applications in medicine and biotechnology. The glycodiversification opens additional applications for DSases. Therefore, the desig

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23

Manley, Karen. "The innovation competence of repeat public sector clients in the Australian construction industry." Construction Management and Economics 24, no. 12 (2006): 1295–304. http://dx.doi.org/10.1080/01446190600934953.

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24

Ostrander, E. A., P. M. Jong, J. Rine, and G. Duyk. "Construction of small-insert genomic DNA libraries highly enriched for microsatellite repeat sequences." Proceedings of the National Academy of Sciences 89, no. 8 (1992): 3419–23. http://dx.doi.org/10.1073/pnas.89.8.3419.

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25

Tanskanen, Jarna, Timo K. Korhonen, and Benita Westerlund-WikstrÃ¯Â¿Â½m. "Construction of a Multihybrid Display System: Flagellar Filaments Carrying Two Foreign Adhesive Peptides." Applied and Environmental Microbiology 66, no. 9 (2000): 4152–56. http://dx.doi.org/10.1128/aem.66.9.4152-4156.2000.

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ABSTRACT A multivalent, bifunctional flagellum carrying two different adhesive peptides in separate flagellin subunits within a filament was constructed in Escherichia coli. The inserted peptides were the fibronectin-binding 115-mer D repeat region of Staphylococcus aureus and the 302-mer collagen-binding region of YadA ofYersinia enterocolitica. Western blotting, immunoelectron microscopy, and adhesion tests with hybrid flagella from an intrans-complemented ΔfliC E. coli strain showed that individual filaments consisted of both recombinant flagellins.

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26

Nelson, Derek W., Alexander Connor, Yu Shen, and Ryan J. Gilbert. "Construction of an Elastin-like Polypeptide Gene in a High Copy Number Plasmid Using a Modified Method of Recursive Directional Ligation." SynBio 2, no. 2 (2024): 174–89. http://dx.doi.org/10.3390/synbio2020010.

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Elastin-like polypeptides (ELPs) are popular biomaterials due to their reversible, temperature-dependent phase separation and their tunability, which is achievable by evolving procedures in recombinant technology. In particular, recursive direction ligation by plasmid reconstruction (PRe-RDL) is the predominant cloning technique used to generate ELPs of varying lengths. Pre-RDL provides precise control over the number of (VPGXG)n repeat units in an ELP due to the selection of type IIS restriction enzyme (REs) sites in the reconstructed pET expression plasmid, which is a low-to-medium copy numb

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27

Jiang, Dai-Lin, Chao-Ling Yao, Nien-Jen Hu та Yung-Chuan Liu. "Construction of a Tandem Repeat Peptide Sequence with Pepsin Cutting Sites to Produce Recombinant α-Melanocyte-Stimulating Hormone". Molecules 26, № 20 (2021): 6207. http://dx.doi.org/10.3390/molecules26206207.

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The production of α-melanocyte-stimulating hormone (α-MSH), a peptide hormone composed of 13 amino acids, is attempted by recombinant expression using E. coli as the host. To achieve this aim, a synthetic gene containing eight tandem repeats of msh gene (8msh) was designed for ribosomal synthesis of 8 α-MSH. The merit of the strategy is to diminish the peptide toxicity against the host cell and to achieve a higher production yield. Pepsin cleavage sites are introduced between the peptides for enzymatic proteolysis to obtain the monomeric peptide of α-MSH. The constructed plasmid was transforme

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Saina, Josphat K., Zhi-Zhong Li, Andrew W. Gichira, Sheila Avoga, Qing-Feng Wang, and Liao Kuo. "The complete plastome of real yellow wood (Podocarpus latifolius): gene organization and comparison with related species." Holzforschung 73, no. 6 (2019): 525–36. http://dx.doi.org/10.1515/hf-2018-0155.

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AbstractPodocarpus latifolius[(Thunb.) R.Br.exMirb.], also known as real yellow wood, is a large evergreen tree with exceptionally high-quality wood. It is a member of the Podocarpaceae family, which includes many species widely grown for wood pulp as well as timber for construction. Despite its importance, studies focusing on its genetic characterization and molecular biology are limited. Therefore, this study reports the complete plastome ofP. latifolius, which is a circular molecule of 134 020 base pairs (bp) in length, lacking a quadripartite structure. TheP. latifoliusplastome encodes 117

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29

Zhu, Wentao. "The Effects of Infrastructure Projects on House Prices and Rents: Evidence from the HS2 Extension Cancellation in the UK." Journal of Sustainable Business and Economics 7, no. 2 (2024): 76. https://doi.org/10.26549/jsbe.v7i2.19155.

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This paper uses the HS2 extension cancellation in November 2021 as a quasi-experiment to study its impact on house prices and rents in Leeds. Using a DiD approach on repeat sales and monthly rents, I compare property values near the HS2 station and proposed construction site before and after the announcement. Results show a 3.6% decrease in house prices and a 3.9% decline in rents near the station, while properties near the construction site experienced a 2.4% increase in prices and a 2.1% rise in rents. This is the frst paper to analyse the HS2 cancellation effect using panel data methods.

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Ning, Han-Bing, Ji-Chang Li, Zhi-Guo Liu, and Dai-Ming Fan. "Construction and expression of telomeric repeat binding factor 2 small interfering RNA eukaryotic vector." World Chinese Journal of Digestology 14, no. 11 (2006): 1044. http://dx.doi.org/10.11569/wcjd.v14.i11.1044.

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WANG, Xiao-Fu. "Construction of vectors for expression of cleavable tandem repeat Thanatin fusion protein in plants." HEREDITAS 29, no. 06 (2007): 758. http://dx.doi.org/10.1360/yc-007-0758.

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32

Davey, James A., and Natalie K. Goto. "TReSR: A PCR-compatible DNA sequence design method for engineering proteins containing tandem repeats." PLOS ONE 18, no. 4 (2023): e0281228. http://dx.doi.org/10.1371/journal.pone.0281228.

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Protein tandem repeats (TRs) are motifs comprised of near-identical contiguous sequence duplications. They are found in approximately 14% of all proteins and are implicated in diverse biological functions facilitating both structured and disordered protein-protein and protein-DNA interactions. These functionalities make protein TR domains an attractive component for the modular design of protein constructs. However, the repetitive nature of DNA sequences encoding TR motifs complicates their synthesis and mutagenesis by traditional molecular biology workflows commonly employed by protein engine

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Sun, Zexin, Hongyuan Mei, Wente Pan, Zhengwei Zhang, and Jie Shan. "A Robotic Arm Based Design Method for Modular Building in Cold Region." Sustainability 14, no. 3 (2022): 1452. http://dx.doi.org/10.3390/su14031452.

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The robotic arm has emerged as an essential tool for the rapid construction of high-quality buildings due to its ability to repeat instructions, achieve precise positioning and fine operations. The robotic arm can also effectively replace workers to complete building construction under low temperature and short daylight conditions. Thus, it can be forward-looking to construct modular buildings in cold region, but how to realize the construction of modular buildings through human-machine coordination and remote operation is a significant issue. This article discusses the feasibility of robotic

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Petrovic, Vladana, Branislava Stoiljković, Nataša Petković, and Hristina Krstić. "Spatial-Functional Organization of a Contemporary Apartment in Serbia." Journal of Mediterranean Cities 3, no. 1 (2023): 31–42. http://dx.doi.org/10.38027/mediterranean-cities_vol3no1_3.

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The expansion of residential construction has marked the last twenty years in Serbia. Contemporary housing construction is popularly called “investor construction”, and it is based on the investor’s influence on achieving the greatest possible capacity in relation to the area of the plot, the largest possible area for sale, as many apartments as possible per floor, the largest possible dimensions of the building on the plot, and the largest possible number of rooms within the smallest possible total square footage. The research’s aim is to discover how the aforementioned effects manifest thems

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Hayden, M. J., S. Khatkar, and P. J. Sharp. "Targetting microsatellites (SSRs) in genetic linkage maps of bread wheat." Australian Journal of Agricultural Research 52, no. 12 (2001): 1143. http://dx.doi.org/10.1071/ar01026.

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The construction of genetic linkage maps from intraspecific crosses of bread wheat is slow and difficult due to very limited levels of polymorphism, which hinder the assignment of linkage groups to chromosomes and leave large genomic regions without markers. Simple sequence repeats (SSRs) reveal a higher incidence of polymorphism and are more informative than any other DNA marker, and are therefore considered a marker of choice for self-pollinating crops with little intraspecific polymorphism. However, the availability of SSRs in bread wheat is still limited. In this study, selectively amplifi

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36

Pogibenko, Tamara. "Serial Constructions in Khmer Syntax: Welcome to Syntactic Jungle." Oriental Courier, no. 2 (2024): 199. http://dx.doi.org/10.18254/s268684310031323-3.

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Serial constructions are a prominent feature of Khmer syntax. Their structural-semantic types are basically the same as we find in other languages. However, due to monosyllabic nature of meaning-bearing units and isolating type of Khmer grammar they acquire structural and functional features which are not found in serial constructions in languages of non-isolating profile. That is the reason why syntactic analysis of a Khmer sentence with serial constructions can be a challenge. The present research deals with such features as multicomponent composition and structural diversity of units compri

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Granstrem, Maria, Milena Zolotareva, and Tatyana Slavina. "High-rise construction in historical cities through the example of Saint Petersburg." E3S Web of Conferences 33 (2018): 01028. http://dx.doi.org/10.1051/e3sconf/20183301028.

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The article sets forth results of the landscape visual analysis of the interaction of high-rise construction facilities with the environment of historical urban spaces. A toxic connection of high-rise construction facilities with the established urban landscape was analyzed and recorded. One of the latest stages of the reconstruction of historical cities, which penetrated many European countries at the end of the 20th century, also started in the beginning of the 21st century in Russia, where the reconstruction of historical facilities and territories became one of the leading trends of archit

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38

Liu, Jianfeng, Bowen Yang, Yuetong Ming, Yuchu Zhang, and Yunqing Cheng. "Identification of Expressed Sequence Tag-simple Sequence Repeat Markers from the De Novo Transcriptome Sequence of Red Raspberry (Rubus idaeus L.)." HortScience 52, no. 4 (2017): 554–59. http://dx.doi.org/10.21273/hortsci11680-16.

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Rubus idaeus has remarkable economic and cultural value. Developing efficient simple sequence repeat (SSR) markers is necessary for the molecular breeding of red raspberry. In this study, SSR mining was performed using the de novo transcriptome sequence of R. idaeus. In total, 14,210 SSR sequences were identified from 11,158 SSR-containing unigenes. In all the SSR sequences, mononucleotide, dinucleotide, and trinucleotide repeats were the most common, and their number and percentage were 1323 (9.31%), 6752 (47.52%), and 4897 (34.46%), respectively. Of the mononucleotide and dinucleotide repeat

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Zang, Mingyue, Qian Su, Yuhao Weng, et al. "Complete Chloroplast Genome of Fokienia hodginsii (Dunn) Henry et Thomas: Insights into Repeat Regions Variation and Phylogenetic Relationships in Cupressophyta." Forests 10, no. 7 (2019): 528. http://dx.doi.org/10.3390/f10070528.

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Fokienia hodginsii (Dunn) Henry et Thomas is a relic gymnosperm with broad application value. It is a fit candidate when choosing species for the construction of artificial forests. We determined the complete chloroplast genome sequence of F. hodginsii, which is 129,534 bp in length and encodes 83 protein genes, 33 transfer RNA (tRNA) genes, as well as four ribosomal RNA genes. The GC content of the complete sequence and protein coding regions is 34.8% and 36.2%, respectively. We identified 11 tandem repeats, 11 forward repeats, and three palindromic repeats and classified them by size. Follow

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Gruner, Alexander, and Michael Huber. "New Combinatorial Construction Techniques for Low-Density Parity-Check Codes and Systematic Repeat-Accumulate Codes." IEEE Transactions on Communications 60, no. 9 (2012): 2387–95. http://dx.doi.org/10.1109/tcomm.2012.070912.110164.

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Senior, M. L., E. C. L. Chin, M. Lee, J. S. C. Smith, and C. W. Stuber. "Simple Sequence Repeat Markers Developed from Maize Sequences Found in the GENBANK Database: Map Construction." Crop Science 36, no. 6 (1996): 1676–83. http://dx.doi.org/10.2135/cropsci1996.0011183x003600060043x.

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Bigornia, Luisa, Kristen M. Lockridge, and Ellen E. Sparger. "Construction and In Vitro Characterization of Attenuated Feline Immunodeficiency Virus Long Terminal Repeat Mutant Viruses." Journal of Virology 75, no. 2 (2001): 1054–60. http://dx.doi.org/10.1128/jvi.75.2.1054-1060.2001.

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ABSTRACT AP-1- and ATF-binding sites are cis-acting transcriptional elements within the U3 domain of the feline immunodeficiency virus (FIV) long terminal repeat (LTR) that serve as targets for cellular activation pathways and may regulate virus replication. We report that FIV LTR mutant proviruses encoding U3 deletions of the ATF-binding sequence exhibited restricted virus expression and replication in both feline lymphocytes and macrophages. In contrast, deletion of the AP-1 site had negligible effects on virus expression and replication. FIV LTR mutant proviruses encoding deletions of both

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Chen, Hui, Yue Song, Lei-Ting Li, et al. "Construction of a High-Density Simple Sequence Repeat Consensus Genetic Map for Pear (Pyrus spp.)." Plant Molecular Biology Reporter 33, no. 2 (2014): 316–25. http://dx.doi.org/10.1007/s11105-014-0745-x.

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Zhang, Yong, Qian Kun Wang, and Qian Huang. "Research on the Construction of Saving-Energy Building Modular System." Advanced Materials Research 689 (May 2013): 110–13. http://dx.doi.org/10.4028/www.scientific.net/amr.689.110.

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After surveying and analyzing many energy-saving building cases, we put forward energy-saving building modular system. According to the system elements which are building types, structure types, building palisade structure system, renewable energy type, energy saving rate, renewable energy rate and capital contribution rate, we divide the system into 18 energy-saving building modules. And this article focuses on the construction principles, system, system composition and system elements purposes. Also we can use this system to design saving-energy buildings with menu type which can not only re

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Mahajanam, Sudhakar, and Krista Heidersbach. "Corrosion Studies of Heat-Tinted Austenitic Stainless Steel." Materials Performance 60, no. 5 (2021): 42–47. https://doi.org/10.5006/mp2021_60_5-42.

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Austenitic stainless steels (SS) are known to pit in chloride-containing waters, but are considered a viable material of construction for freshwater service. Within industry, repeat failures have occurred on newly installed equipment, often tying the failures to effects from surface finishing on corrosion initiation. Inspired by real life failures, this work utilizes electrochemical and immersion testing to study the effects of iron contamination and heat tint on localized corrosion initiation in welded SS.

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Honma, Kiyonobu, Howard K. Kuramitsu, Robert J. Genco, and Ashu Sharma. "Development of a Gene Inactivation System forBacteroides forsythus: Construction and Characterization of a BspA Mutant." Infection and Immunity 69, no. 7 (2001): 4686–90. http://dx.doi.org/10.1128/iai.69.7.4686-4690.2001.

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ABSTRACT Bacteroides forsythus is a gram-negative anaerobic bacterium associated with periodontitis. The bspA gene encoding a cell surface associated leucine-rich repeat protein (BspA) involved in adhesion to fibronectin and fibrinogen was recently cloned from this bacterium in our laboratory. We now describe the construction of a BspA-defective mutant of B. forsythus. This is the first report describing the generation of a specific gene knockout mutant of B. forsythus, and this procedure should be useful in establishing the identity of virulence-associated factors in these organisms.

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Stuhlmann, H., R. Jaenisch, and R. C. Mulligan. "Construction and properties of replication-competent murine retroviral vectors encoding methotrexate resistance." Molecular and Cellular Biology 9, no. 1 (1989): 100–108. http://dx.doi.org/10.1128/mcb.9.1.100-108.1989.

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A series of replication-competent Moloney murine leukemia virus vectors was constructed in which each vector contained a mutant dihydrofolate reductase (DHFR) cDNA insert in the U3 region of the viral long terminal repeat. Two of the resulting viruses, MLV (murine leukemia virus) DHFR*-5 and MLV DHFR*-7, were able to stably transfer methotrexate resistance to infected fibroblast cells upon multiple rounds of virus replication and in the absence of drug selection. Cell lines producing recombinant virus with high titers were established, which indicated that the insert did not grossly interfere

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Stuhlmann, H., R. Jaenisch, and R. C. Mulligan. "Construction and properties of replication-competent murine retroviral vectors encoding methotrexate resistance." Molecular and Cellular Biology 9, no. 1 (1989): 100–108. http://dx.doi.org/10.1128/mcb.9.1.100.

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A series of replication-competent Moloney murine leukemia virus vectors was constructed in which each vector contained a mutant dihydrofolate reductase (DHFR) cDNA insert in the U3 region of the viral long terminal repeat. Two of the resulting viruses, MLV (murine leukemia virus) DHFR*-5 and MLV DHFR*-7, were able to stably transfer methotrexate resistance to infected fibroblast cells upon multiple rounds of virus replication and in the absence of drug selection. Cell lines producing recombinant virus with high titers were established, which indicated that the insert did not grossly interfere

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Bi, Kang, Xinyu Shi, Da Wan, et al. "Research on Automated On-Site Construction of Timber Structures: Mobile Construction Platform Guided by Real-Time Visual Positioning System." Buildings 15, no. 10 (2025): 1594. https://doi.org/10.3390/buildings15101594.

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In recent years, the AEC industry has increasingly sought sustainable solutions to enhance productivity and reduce environmental pollution, with wood emerging as a key renewable material due to its excellent carbon sequestration capability and low ecological footprint. Despite significant advances in digital fabrication technologies for timber construction, on-site assembly still predominantly relies on manual operations, thereby limiting efficiency and precision. To address this challenge, this study proposes an automated on-site timber construction process that integrates a mobile constructi

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Jaśkowski, Piotr. "Repetitive construction processes scheduling using mixed-integer linear programming." Budownictwo i Architektura 14, no. 2 (2015): 055–61. http://dx.doi.org/10.35784/bud-arch.1649.

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Many construction projects contain several identical or similar units, such as floors in multistory buildings, houses in housing developments, sections of pipelines or highways. Repetitive processes arise from the subdivision of a generalized construction process into specific activities associated with particular units. In many cases it is possible to perform individual processes (repeated in each units) in alternative ways (modes). Regardless of the construction project procurement system, duration and cost are the key factors determining project’s economic efficiency and fulfillment of the

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