Academic literature on the topic 'Reporter luciferase assay'
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Journal articles on the topic "Reporter luciferase assay"
Kenda, Maša, Jan Vegelj, Barbara Herlah, Andrej Perdih, Přemysl Mladěnka, and Marija Sollner Dolenc. "Evaluation of Firefly and Renilla Luciferase Inhibition in Reporter-Gene Assays: A Case of Isoflavonoids." International Journal of Molecular Sciences 22, no. 13 (2021): 6927. http://dx.doi.org/10.3390/ijms22136927.
Full textSiebring-van Olst, Ellen, Christie Vermeulen, Renee X. de Menezes, Michael Howell, Egbert F. Smit, and Victor W. van Beusechem. "Affordable Luciferase Reporter Assay for Cell-Based High-Throughput Screening." Journal of Biomolecular Screening 18, no. 4 (2012): 453–61. http://dx.doi.org/10.1177/1087057112465184.
Full textHancock, Michael K., Myleen N. Medina, Brendan M. Smith, and Anthony P. Orth. "Microplate Orbital Mixing Improves High-Throughput Cell-Based Reporter Assay Readouts." Journal of Biomolecular Screening 12, no. 1 (2006): 140–44. http://dx.doi.org/10.1177/1087057106296046.
Full textWu, Chun, Chie Suzuki-Ogoh, and Yoshihiro Ohmiya. "Dual-reporter assay using two secreted luciferase genes." BioTechniques 42, no. 3 (2007): 290–92. http://dx.doi.org/10.2144/000112428.
Full textCheng, Zhijie, Denise Garvin, Aileen Paguio, Pete Stecha, Keith Wood, and Frank Fan. "Luciferase Reporter Assay System for Deciphering GPCR Pathways." Current Chemical Genomics 4 (December 21, 2010): 84–91. http://dx.doi.org/10.2174/1875397301004010084.
Full textBarriscale, Kathy A., Sharon A. O’Sullivan, and Tommie V. McCarthy. "A single secreted luciferase-based gene reporter assay." Analytical Biochemistry 453 (May 2014): 44–49. http://dx.doi.org/10.1016/j.ab.2014.02.019.
Full textKolb, Alfred J., and Kenneth Neumann. "Luciferase Measurements in High Throughput Screening." Journal of Biomolecular Screening 1, no. 2 (1996): 85–88. http://dx.doi.org/10.1177/108705719600100207.
Full textHannah, Rita R., Martha L. Jennens-Clough, and Keith V. Wood. "MipTec Proceedings: Transcriptional Assay Screens Using Renilla Luciferase as an Internal Control." JALA: Journal of the Association for Laboratory Automation 3, no. 4 (1998): 41–44. http://dx.doi.org/10.1177/221106829800300406.
Full textDidiot, Marie-Cecile, Sergio Serafini, Martin J. Pfeifer, Frederick J. King, and Christian N. Parker. "Multiplexed Reporter Gene Assays: Monitoring the Cell Viability and the Compound Kinetics on Luciferase Activity." Journal of Biomolecular Screening 16, no. 7 (2011): 786–93. http://dx.doi.org/10.1177/1087057111407768.
Full textNieuwenhuijsen, Bart W., Youping Huang, Yuren Wang, Fernando Ramirez, Gary Kalgaonkar, and Kathleen H. Young. "A Dual Luciferase Multiplexed High-Throughput Screening Platform for Protein-Protein Interactions." Journal of Biomolecular Screening 8, no. 6 (2003): 676–84. http://dx.doi.org/10.1177/1087057103258287.
Full textDissertations / Theses on the topic "Reporter luciferase assay"
Wiencierz, Anne Maria. "Entwicklung eines Dual-Luciferase-Reportergen-Assays zum Nachweis der Induktion antioxidativer Enzyme durch Nahrungsbestandteile." Master's thesis, Universität Potsdam, 2008. http://opus.kobv.de/ubp/volltexte/2009/2790/.
Full textThe induction of antioxidative enzymes might be an opportunity to elevate the cellular antioxidative capacity and, thus, to prevent oxidative stress associated diseases (e. g. cardio-vascular disease, neurodegenerative disease, atherosclerosis). Based on this idea the dual luciferase reporter gene (DLR) assay was developed to demonstrate the induction of three antioxidative enzymes: catalase (CAT), cytosolic glutathione peroxidase (GPX1), and copper-zinc superoxide dismutase (SOD1). In the course of the development three mammalian cell lines (CaCo2, IEC-18, V79) were tested for their ability to serve as a model cell line. The line V79 was chosen due to the transfection efficiency. To give consideration to a high-throughput several parameters were studied (e. g. format of the cultural plates, amount of DNA, kinetics of the luciferases) and the DLR assay was successfully established in 96 well plates. Subsequently, L-carnitine, catechin, epigallocatechin gallate, genistein, hydrogen peroxide (H2O2), sodium ascorbate, paraquat, quercetin, 12-O-tetradecanoylphorbol-13-acetate (TPA) and trolox were tested in non-cytotoxic concentrations for the activation of the rat CAT, human GPX1 and human SOD1 promoter. The maximally tolerable concentrations were determined by resazurin test in advance. Three out of these ten compounds were identified as potent inducers of GPX1 and SOD1. Stimulation of reporter gene construct transient transfected V79 cells for 24 hours with 100 µM paraquat caused a duplication of the relative GPX1 promoter activity and a 1.6-/1.7-fold increase of the relative SOD1 promoter activity. The incubation with 20 µM gen-istein or 10 µM quercetin resulted in duplication to triplication of both, the relative GPX1 and SOD1 promoter activity. In contrast, the rat CAT promoter was activated by 50 µM H2O2 (1.5-fold). Consequently, genistein, quercetin, and H2O2 are considered to be suitable reference substances for this DLR assay. To further characterize the inducing potential of the tested compounds all of them should be tested in different concentrations. Furthermore, for the routinely performed DLR assay it is recommended to use stably transfected cells to eliminate transfection caused variations.
Zheng, Qiaoyun. "Study of Cancer Related Proteins: LRG-1 and PD-L1." Cleveland State University / OhioLINK, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=csu1495735827467521.
Full textLai, John. "Functional analyses of polymorphisms in the promoters of the KLK3 and KLK4 genes in prostate cancer." Queensland University of Technology, 2006. http://eprints.qut.edu.au/16454/.
Full textHofmann, Peter Josef. "Aufbau eines Reportergenassays zur Untersuchung der Wechselwirkung endokriner Disruptoren mit der T 3-regulierten Transaktivierung." Doctoral thesis, Humboldt-Universität zu Berlin, Mathematisch-Naturwissenschaftliche Fakultät I, 2008. http://dx.doi.org/10.18452/15813.
Full textTriiodothyronine (T3) is a crucial regulator of many physiological processes during development, growth and metabolism. A variety of natural and synthetic substances, which are collectively termed endocrine disrupters (ED) due to their interference with the endocrine system, is taken up on a daily base. A novel luciferase-based T3-responsive reporter gene system employing a human liver cell line transfected with thyroid hormone receptor (TR) alpha1 was established in this work to elucidate the potential molecular interference of certain ED with TR and their transcriptional activity. This assay was validated to be a highly sensitive and reliable tool for analyzing agonistic and antagonistic effects of test compounds using the synthetic TR agonist GC-1 and the antagonist NH-3. Dose-response data of test compounds contained in food, cosmetics, pesticides, plasticizers and other industrial chemicals were obtained after applying the substances alone in activation assays or in combination with T3 in competition assays. In total 21 test compounds were screened of which some acted as pure agonists or antagonists while others were mixed agonists/antagonists in the micromolar concentration range and only one was without effect. Follow-up studies are recommended for some of these substances with regard to their effects as determined in this bioassay and in light of information known on human exposure, i.e., 4-methylbenzyliden camphor, 4-nonylphenol, acetochlor, benzophenone 2, benzophenone 3, bisphenol A, genistein, octylmethoxycinnamate, tetrabromobisphenol A and xanthohumol. In addition some endogenous metabolites of thyroid hormones were surprisingly potent agonists in the T3 reporter gene assay and merit further attention. The novel bioassay established here represents a reliable tool for the screening and molecular characterization of substances interfering with T3-mediated transactivation of gene expression.
Dukorn, Stefanie [Verfasser], та A. [Akademischer Betreuer] Buschauer. "Pharmacological tools for the NPY receptors: [³⁵S]GTPγS binding assays, luciferase gene reporter assays and labeled peptides / Stefanie Dukorn ; Betreuer: A. Buschauer". Regensburg : Universitätsbibliothek Regensburg, 2018. http://d-nb.info/115170007X/34.
Full textDouvris, Adrianna. "Functional Analysis of the TRIB1 Locus in Coronary Artery Disease." Thèse, Université d'Ottawa / University of Ottawa, 2011. http://hdl.handle.net/10393/20115.
Full textLu, Yu-Jou, and 陸羽柔. "Analysis of HHP1 Promoter Using Luciferase Reporter Assay System." Thesis, 2011. http://ndltd.ncl.edu.tw/handle/54762340551517489882.
Full text國立臺灣大學
生化科技學系
99
HHP1 (heptahelical transmembrane protein 1), a protein with a predicted seven transmembrane domain structure homologous to mPRs (membrane progestin receptors), is determined to be a novel negative regulator in ABA and osmotic signaling. Sequence analysis showed that the fragment upstream to the coding region of HHP1 (5’ UTR + -1 ~ -1552 bp) contains many ABA-, dehydration-, salinity- and low temperature-responsive elements. In this study, we tried to investigate the stress-responsive elements which are involved in HHP1 transcriptional regulation using Dual-Luciferase Reporter○R (DLRTM ) Assay System (Promega). Four different DNA fragments containing various length of the putative promoter region of HHP1 (U1, 5’ UTR + -1 ~ -1552 bp; U2, 5’ UTR + -1 ~ -1103 bp; U3, 5’ UTR + -1 ~ -740 bp and U4, 5’ UTR + -1 ~ -302 bp) were cloned into vectors harboring firefly luciferase (FLUC). These constructs (U1 ~ U4-pSP) were co-transfected into protoplasts isolated from Arabidopsis mesophyll with internal control plasmid 35S-pRL expressing Renilla luciferase (RLUC). Due to the methods used to isolate and transfect plasmid into protoplasts greatly affected the assay, a few modification were made to optimize the protoplasts isolation and transfection. Protoplasts isolated from 5 ~ 7 week-old Arabidopsis, 2 × 106 cells/MMG solution for transfection, and 100:1 mass ratio of U1 ~ U4-pSP to 35S-pRL gave the best transformation and normalized luciferase expression (FLUC/RLUC). From more than three independent experiments, higher luciferase expressions were observed in the U4-pSP construct which contains the shorter HHP1 upstream fragment. The experiments were also performed when the protoplasts were treated with abscisic acid. A few interesting preliminary results were discussed.
Lin, Yun-Tong, and 林筠彤. "The small RNA of Japanese encephalitis virus inhibits translation efficiency analyzed by a luciferase reporter gene assay." Thesis, 2008. http://ndltd.ncl.edu.tw/handle/82w7a2.
Full text國立東華大學
生物技術研究所
96
Japanese encephalitis virus (JEV) contains a single positive-strand RNA genome of 10,976 nucleotides in length. The 5’- and 3’-terminal regions contain conserved and highly ordered secondary structures that have been shown to play important roles in viral translation and replication. Previous studies in our laboratory have shown the abundant accumulation of a 3’-terminal 521- to 523-nucleotide genome fragment, representing the highly conserved region of the 3’-untranslated region, in JEV-infected cells. Function of the small RNA remains unclear. The small RNA was detected in Dengue Type 2 virus-infected cells in this study suggesting the small RNA may exist among all flaviviruses. The molar ratio of the small RNA to the genome is greater in JEV persistently-infected cells (3.36; survival cells from acutely infection, approximately 100 days postinfection) than those in JEV acutely-infected cells (0.93; 2 days postinfection prior to cell death) indicated that the small RNA plays a role in establishment of persistent infection. A small portion of viral genomic RNA was found in the nuclear fraction, while the small RNA was only detected in the cytoplasmic fraction. An RNA interference detection assay was used to address possible effect of the small RNA in this cellular RNA degradation pathway but the results showed no significant effect. Surprisingly, the small RNA alone inhibits the translation efficiently of the firefly luciferase. In addition, a Renilla luciferase (Rluc) gene reporter in-frame fused with the 5’ and 3’ terminal sequences of JEV resulting as a JEV minicon and from this, the synthetic RNA transcript was made for viral translation assay. This JEV minicon was proved to be cap-dependent translation. Transfecting of sense- or anti-sense small RNA both inhibit JEV minicon translation efficiency, suggesting that the small RNA plays a role in viral translation.
Pohlová, Lenka. "Onkogenní promotor c-myc jako cíl pro nový typ heterocyklických dikationtů stabilizujících G-kvadruplex." Master's thesis, 2015. http://www.nusl.cz/ntk/nusl-353836.
Full textKožantová, Jana. "Měření aktivace signálních drah v myší makrofágové linii IC-21 a primárních dendritických buňkách po infekci virem klíšťové encefalitidy." Master's thesis, 2017. http://www.nusl.cz/ntk/nusl-367677.
Full textBook chapters on the topic "Reporter luciferase assay"
Wettey, Frank R., and Antony P. Jackson. "Luciferase reporter assay." In Subcellular Biochemistry. Springer Netherlands, 2006. http://dx.doi.org/10.1007/978-1-4020-4896-8_39.
Full textTomasello, Luisa, Landon Cluts, and Carlo M. Croce. "Experimental Validation of MicroRNA Targets: Luciferase Reporter Assay." In Methods in Molecular Biology. Springer New York, 2019. http://dx.doi.org/10.1007/978-1-4939-9207-2_17.
Full textMontefiori, David C. "Measuring HIV Neutralization in a Luciferase Reporter Gene Assay." In Methods in Molecular Biology. Humana Press, 2009. http://dx.doi.org/10.1007/978-1-59745-170-3_26.
Full textJin, Yi, Zujian Chen, Xiqiang Liu, and Xiaofeng Zhou. "Evaluating the MicroRNA Targeting Sites by Luciferase Reporter Gene Assay." In MicroRNA Protocols. Humana Press, 2012. http://dx.doi.org/10.1007/978-1-62703-083-0_10.
Full textMarcos-Vadillo, Elena, and Asunción García-Sánchez. "Promoter Assay Using Luciferase Reporter Gene in the A549 Cell Line." In Methods in Molecular Biology. Springer New York, 2016. http://dx.doi.org/10.1007/978-1-4939-3652-6_14.
Full textIglesias-Ara, Ainhoa, Nerea Osinalde, and Ana M. Zubiaga. "Detection of E2F-Induced Transcriptional Activity Using a Dual Luciferase Reporter Assay." In Methods in Molecular Biology. Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-7565-5_14.
Full textVincelli, Gabriele, and Andrea Bedini. "Renilla Luciferase Reporter Assay to Study 3′UTR-Driven Posttranscriptional Regulations of OPRM1." In Methods in Molecular Biology. Springer New York, 2014. http://dx.doi.org/10.1007/978-1-4939-1708-2_4.
Full textMüller, Katharina, Manfred Ogris, and Haider Sami. "Firefly Luciferase-Based Reporter Gene Assay for Investigating Nanoparticle-Mediated Nucleic Acid Delivery." In Nanotechnology for Nucleic Acid Delivery. Springer New York, 2019. http://dx.doi.org/10.1007/978-1-4939-9092-4_15.
Full textVincelli, Gabriele, and Andrea Bedini. "Renilla Luciferase Reporter Assay to Study 3′-UTR-Driven Posttranscriptional Regulation of OPRM1." In Methods in Molecular Biology. Springer US, 2020. http://dx.doi.org/10.1007/978-1-0716-0884-5_2.
Full textLieber, Diana, and Susanne M. Bailer. "Determination of HSV-1 Infectivity by Plaque Assay and a Luciferase Reporter Cell Line." In Methods in Molecular Biology. Humana Press, 2013. http://dx.doi.org/10.1007/978-1-62703-601-6_12.
Full textConference papers on the topic "Reporter luciferase assay"
Shi, Yihui, Jaehyeon Park, Chandraiah Lagisetti, Wei Zhou, Lidia C. Sambucetti, and Thomas R. Webb. "Abstract 4193: A triple exon-skipping luciferase reporter assay identifies a new CLK inhibitor scaffold." In Proceedings: AACR Annual Meeting 2017; April 1-5, 2017; Washington, DC. American Association for Cancer Research, 2017. http://dx.doi.org/10.1158/1538-7445.am2017-4193.
Full textFields, Wanda R., Wolfgang Liedtke, Jinju Li, and Betsy Bombick. "Abstract 1693: Assessment of Nrf2 luciferase reporter assay and heme oxygenase expression as models of oxidative stress inin vitrocultures of lung cells exposed to cigarette smoke condensate." In Proceedings: AACR 101st Annual Meeting 2010‐‐ Apr 17‐21, 2010; Washington, DC. American Association for Cancer Research, 2010. http://dx.doi.org/10.1158/1538-7445.am10-1693.
Full textLiu, Haiyun, Brian Della Fera, John Foulke, Luping Chen, and Fang Tian. "Abstract 1306: Primary NK cells and luciferase expressing reporter cell lines for use in developing ADCC assays for immuno-oncology drug screening." In Proceedings: AACR Annual Meeting 2021; April 10-15, 2021 and May 17-21, 2021; Philadelphia, PA. American Association for Cancer Research, 2021. http://dx.doi.org/10.1158/1538-7445.am2021-1306.
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