Academic literature on the topic 'Reproductive Techniques'
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Journal articles on the topic "Reproductive Techniques"
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Bharti, Alok, Sailabala Dei, and Santosh Kumar. "Livestock Reproductive Techniques." Current Journal of Applied Science and Technology 31, no. 2 (December 8, 2018): 1–11. http://dx.doi.org/10.9734/cjast/2018/45891.
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Sharkey, Steve, Robert J. Callan, Robert Mortimer, and Cleon Kimberling. "Reproductive Techniques in Sheep." Veterinary Clinics of North America: Food Animal Practice 17, no. 2 (July 2001): 435–55. http://dx.doi.org/10.1016/s0749-0720(15)30037-2.
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Praxedes, Érica Camila Gurgel, Gislayne Christianne Xavier Peixoto, Andréia Maria da Silva, and Alexandre Rodrigues Silva. "Reproduction in agouti (Dasyprocta spp.): A review of reproductive physiology for developing assisted reproductive techniques." Animal Reproduction 15, no. 4 (2018): 1181–92. http://dx.doi.org/10.21451/1984-3143-ar2018-0058.
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Davidson, Autumn, and Bruce Eilts. "Advanced Small Animal Reproductive Techniques." Journal of the American Animal Hospital Association 42, no. 1 (January 1, 2006): 10–17. http://dx.doi.org/10.5326/0420010.
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Small animal reproductive techniques have become quite advanced in recent years. Techniques described in this paper include transcervical catheterization for the purpose of insemination and intrauterine diagnostics (e.g., cytology, culture, and/or biopsy), and semen cryopreservation that allows international shipping of valuable semen and preservation of reproductive capacity. The use of uterine and fetal monitoring devices that enable intervention and mediation of dystocia is also described.
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Dowbór-Dzwonka, Aneta, Bernadeta Cegła, Małgorzata Filanowicz, and Ewa Szymkiewicz. "Assisted reproductive techniques and NaProTechnology." Zdrowie Publiczne 122, no. 3 (July 1, 2012): 322–28. http://dx.doi.org/10.12923/j.0044-2011/122/3/a.19.
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Bretzlaff, Katherine N., and Juan E. Romano. "Advanced Reproductive Techniques in Goats." Veterinary Clinics of North America: Food Animal Practice 17, no. 2 (July 2001): 421–34. http://dx.doi.org/10.1016/s0749-0720(15)30036-0.
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Schenker, Joseph G., and Yossef Ezra. "Complications of assisted reproductive techniques." Fertility and Sterility 61, no. 3 (March 1994): 411–22. http://dx.doi.org/10.1016/s0015-0282(16)56568-6.
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Hinrichs, Katrin. "Assisted reproductive techniques in mares." Reproduction in Domestic Animals 53 (September 2018): 4–13. http://dx.doi.org/10.1111/rda.13259.
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Johnson, María Cecilia. "Assisted Reproductive Techniques and Catholicism(s) in the US." Religion and Gender 9, no. 2 (December 10, 2019): 147–69. http://dx.doi.org/10.1163/18785417-00902001.
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Abstract Assisted Reproductive Techniques (ART s) have proposed a new way of understanding notions of sexuality, reproduction, gestation, and family, and these transformations have arguably been a challenge in the religious field. This study aims to analyze the stances taken within the Catholic spectrum in the United States on ART s. Catholicism in the United States is an internally heterogeneous space, and different agents have taken diverse stances on ART s, with an impact on health care regulations, Catholic facilities administrations, and Catholics’ and non-Catholics’ reproductive rights. Drawing from a qualitative, interpretive, and documentary analysis of three organizations (the US Conference of catholic bishops, the Catholic Health Organization, and Catholics for Choice), this article proposes some guidelines to analyze and understand the arguments and strategies of various Catholics actors in the United States regarding reproductive healthcare and ART s in the United States.
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Chateauneuf, Doris. "Projet familial, infertilité et désir d’enfant : usages et expériences de la procréation médicalement assistée en contexte québécois." Enfances, Familles, Générations, no. 15 (March 2, 2012): 61–77. http://dx.doi.org/10.7202/1008146ar.
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Les couples qui rencontrent des problèmes d’infertilité et qui cherchent une solution pour leur désir d’enfant se tournent pour la plupart vers la médecine reproductive, celle-ci faisant office d’autorité quant aux questions relatives à la grossesse et à la reproduction. La valorisation de l’enfant, tant sur le plan social qu’au sein de la conjugalité, intervient dans le vécu relatif à l’infertilité et influence le regard que posent les couples sur les nouvelles technologies reproductives. À ce titre, l’étude des relations entre les médecins et les couples infertiles met en évidence les différents discours qui circulent sur la reproduction et fait aussi état des débats et enjeux liés au développement des techniques reproductives et à leur accès grandissant.
Dissertations / Theses on the topic "Reproductive Techniques"
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Inzunza, José. "New micromanipulative techniques in reproductive biology /." Stockholm, 2003. http://diss.kib.ki.se/2003/91-7349-568-9/.
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Wilson, Poe Emma. "Vitrification of day 5/6 human morulas/blastocysts: A 10 year retrospective study in a private assisted reproductive techniques [ART] clinic." Thesis, Stellenbosch : Stellenbosch University, 2015. http://hdl.handle.net/10019.1/96876.
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Thesis (MMed)--Stellenbosch University, 2015.ENGLISH ABSTRACT: This study was designed to retrospectively evaluate the established embryo vitrification/warming programme currently implemented at Drs Aevitas Institute for Reproductive Medicine and to look at factors that might play a role in optimizing the pregnancy outcomes thereof. Vitrification is the achievement of a “state of suspended animation” wherein molecular translational motions are arrested without structural reorganization of the liquid. In embryo vitrification it involves placement of the embryo in a very small volume of vitrification medium that must be cooled at extremely high cooling rates. The vitrification medium contains cryoprotectants to prevent any cryoinjury from occurring to the embryo. This process was initially proposed to effectively manage supernumerary embryos, but it has also provided a viable method of reducing costs for additional embryo transfers as well as the reduction of the incidence of multiple births. Patients who are at risk of ovarian hyper stimulation syndrome (OHSS) can also have all of their embryos vitrified in advance to reduce the likelihood of adverse clinical symptoms if a pregnancy is established. Throughout the period in which vitrification has been in practice, there have been advances in technology as well as continual research being conducted to establish whether newly suggested techniques do, in fact, optimize the outcomes of vitrification. Focus has subsequently been applied to the carrier device used for vitrification, the day on which the embryos are vitrified and stored, as well as the number of embryos transferred in each respective cycle, all to ensure the most favourable outcome. This retrospective study confirmed the use of the Cryotop® as the most viable carrier device for successful survival and pregnancy outcomes. Transfer of day 5 vitrified embryos resulted in significantly higher pregnancy rates compared to day 6 vitrified embryos. Results also indicated that the number of embryos transferred does indeed have a significant effect on the pregnancy outcome and consequently we can possibly argue against the implementation of single embryo transfer in the vitrification programme. Investigation into the effect of female age, specifically oocyte age, on each of these categories indicated that reduced age can be associated with optimal outcomes; however this could not be proven statistically in this cohort of patients. To further look at optimization of the vitrification/warming programme, a Literature Survey was conducted to ascertain the results after Assisted Hatching in frozen/warmed human embryos. Assisted Hatching has been proposed as a solution to Zona Pellucida hardening, which has been found to occur during vitrification. The need for further studies and a meta-analysis of the literature is confidently proposed, as well as a Prospective Study to evaluate the effect of Laser Assisted Hatching in the human blastocyst vitrification/warming programme at Drs Aevitas Institute for Reproductive Medicine.
AFRIKAANSE OPSOMMING: Hierdie studie is ontwerp om die gevestigde embrio vitrifikasie/ontdooi program by Drs Aevitas Instituut vir Reproduktiewe Medisyne, retrospektief te evalueer en die faktore te optimaliseer wat swangerskap uitkomste kan beïnvloed. Vitrifikasie is die proses waardeur die molekulere aktiwiteit binne die embrio in ‘n staat van arres gehou word sonder om die strukture binne die sitplasma te versteur. Dit behels die plasing van ʼn embrio in 'n klein hoeveelheid vitrifikasie medium wat teen 'n hoë tempo afgekoel word. Die vitrifikasie medium bevat kriobeskermmiddels wat die embrio tydens die vitrifikasie proses teen moontlike skade beskerm. Hierdie proses is aanvanklik voorgestel om oortollige embrio’s doeltreffend te bestuur. Dit bied ʼn koste effektiewe metode vir embrio terugplasing, en verlaag die insidensie van veelvoudige swangerskap. Vitrifikasie bied pasiënte met ʼn hoë risiko vir ovariale hiperstimulasiesindroom (OHSS) ‘n alternatief om nadelige kliniese simptome te vermy indien ʼn swangerskap bereik word. Tegnologiese vordering en voortdurende navorsing ondersoek voortdurend nuwe tegnieke vitrifikasie uitkomste te optimaliseer. Fokus word geplaas op die draertoestel wat gebruik word vir vitrifikasie, die dag waarop die embrio's gevitrifiseer en gestoor word, sowel as die aantal embrio’s wat met elke vitrifikasie siklus teruggeplaas word. Hierdie retrospektiewe studie het bevestig dat die gebruik van die Cryotop® die mees suksesvolle toestel vir oorlewing en swangerskap uitkomste is. Die terugplasing van dag 5 gevitrifiseerde embrios het beduidende hoër swangerskapsyfers as dag 6 embrios tot gevolg gehad. Die resultate het ook aangedui dat die aantal embrio's wat teruggeplaas word 'n beduidende uitwerking op die swangerskapsyfer het. Daar kan dus moontlik teen die implementering van 'n enkel embrio-terugplasing neiging in die vitrifikasie program geargumenteer word. Resultate het ook getoon dat optimale uitkomste verwant is aan ʼn laer oösiet ouderdom, alhoewel dit nie in die groep pasiente statisties bewys kon word nie. 'n Literatuurstudie oor AH (Assisted Hatching) op gevitrifiseerde/ontdooide menslike embrio’s is uitgevoer om die vitrifikasie/ontdooi program verder te optimaliseer. AH bied ‘n oplossing vir Zona pellucida verharding, wat tydens vitrifikasie plaasvind. Verdere studies, 'n meta-analise van die literatuur, sowel as 'n prospektiewe studie om die effek van laser AH in gevitrifiseerde/ontdooide menslike blastosiste by Drs Aevitas Instituut vir reproduktiewe medisyne te evalueer, word voorgestel.
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Tompkins, Keith Brian. "Reproductive Manipulation and Larval Rearing Techniques of Yellow Perch (Perca flavescens)." Thesis, Virginia Tech, 1999. http://hdl.handle.net/10919/42112.
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This thesis was conducted to evaluate the potential of obtaining yellow perch eggs and fry by controlling the reproductive cycle of broodfish and the techniques used to rear the resulting fry. Chapter 1 is a review of literature pertaining to yellow perch culture and related topics. Chapter 2 is a study of yellow perch broodstock exposed to a 6-month, artificial photothermal cycle and injected with LHRHa and pimozide to induce spawning. The mean gonadosomatic index for manipulated females was 24.2% and the mean fertility of eggs from manipulated females was 26.5%. The fertilization rate was significantly greater among strip-spawned females than tank spawned females (p < 0.05). The time to ovulation after injection showed a negative correlation (r2 = 0.505) with the mean oocyte diameter of manipulated females.
Chapter 3 is a study comparing the performance of larval yellow perch reared in a recirculating system fed only live rotifers (T1), in a recirculating system fed live rotifers and an artificial diet (T2) and in a greenwater system (T3). Survival was low in all treatments. The specific growth rate did not differ among treatments over the first 6 days. The percentage of fry with inflated swimbladders and absorbed yolksacs did not differ by day 6. While T3 larvae began ingesting food earlier than T1 and T2, the percentage of fry ingesting food did not differ among treatments by day 6 (p >0.1). The total percentage of skeletal deformities was greater in T3 than either T1 or T2 (p < 0.05) and may have been due to excessive handling of T3 larvae during hatch-out.
Chapter 4 outlines the costs associated with a yellow perch hatchery designed around photothermally manipulated broodstock. This hatchery model produces four separate spawns annually, yielding 644,000 fingerlings year-1. Including start-up, fixed and variable costs, such a facility can produce 3" (76mm) fingerlings for $0.20 fingerling-1 for the first 5 years of operation, $0.16 fingerling-1 for the second 5 years and $0.06 fingerling-1 after 10 years.Master of Science
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Ryan, Mandy. "Economics and the patient's utility function : an application to assist reproductive techniques." Thesis, University of Aberdeen, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.282688.
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This thesis questions the assumption that health outcome is the only relevant argument in the patient's utility function. Following this, consideration is given to deciding which methods to use to establish what attributes there are in the patient's utility function and the relative weights to these attributes. Two methods are assessed and considered appropriate: contingent valuation and conjoint analysis. These methods are applied to assess the benefits from assisted reproductive techniques. An attempt is made to determine both the presence and relative importance of health and non-health attributes in the infertile person's utility function. The results from these empirical studies provide evidence of both the potential importance of factors beyond having a child and the sensitivity and applicability of the tools used to establish the relative importance of possible utility bearing attributes in the infertile person's utility function. The factors identified include: knowing you have done everything possible to have a child, the psychological feeling of disappointment, overall satisfaction with life following involvement with assisted reproductive techniques, the provision of follow-up support, and process type attributes such as continuity of care, attitudes of medical staff, waiting time and cost. Health economists concerned with developing economic instruments to measure utility from assisted reproductive techniques, and health care interventions more generally, should find the results useful. So too should those concerned with policy issues around the provision of assisted reproductive techniques and health care interventions more generally.
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Vinoos, Latiefa. "Out of pocket payment for assisted reproductive techniques: How to households recover?" Master's thesis, University of Cape Town, 2017. http://hdl.handle.net/11427/27549.
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Introduction: The cost of ART remains amongst the most prevalent barriers to treatment, especially in resource limited countries where many people are poor and inadequately covered by private and public health insurances. This study aims to assess the financial consequences of out of pocket payment for ART in the South African setting and the ability of couples to financially recover. Methods: A prospective follow-up study was carried out at the Infertility Clinic of the Reproductive Medicine Unit, Groote Schuur Hospital and Faculty of Health Sciences, University of Cape Town. All 135 participants from the original study were invited to participate with no exclusion criteria. A six part questionnaire, developed for the original study, was adjusted to assess recovery from out of pocket expenditure for ART. Indicators of recovery included the recuperation of savings, settlement of debt and reacquisition of sold assets. Persistence of coping strategies such as reduction in spending and additional work was also assessed. Results: A follow-up rate of 54% percent was achieved. The minimum and maximum follow up period was three and five years respectively. Nineteen percent of couples reported complete financial recovery, assessed as the recovery of savings, repayment of all debt and recovery of a sold asset. Forty percent of couples were unable to settle their debt incurred during the original study. The average amount still owed was R 7 750 (SD R5 140). At follow up, 75% of couples who had reduced expenditure to offset the original cost of ART were still reporting a reduction in expenditure while 39% were still engaged in additional work. The majority of couples reporting difficulties at the time of follow up in paying bills or for basic amenities and healthcare were from the poorest socioeconomic tertiles with 64% of all couples indicating that they were not coping financially at the time of follow up. Conclusion: This study documented a long-lasting impact of OPP for ART among all HH but especially among the poorest. Given the high prevalence of infertility, its impact on individuals, couples and communities, the associated mental, emotional and financial consequences, and existing barriers to adequate and affordable treatment should be minimised as South Africa is moving towards the implementation of a national health system.
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Patterson, Abby Rae. "Evaluation of porcine respiratory and reproductive syndrome virus (PRRSV) ante-mortem diagnostic techniques." [Ames, Iowa : Iowa State University], 2007.
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Filetto, Juliana Nicolau. "Vivencias de homens e mulheres apos o fracasso da fertilização in vitro." [s.n.], 2009. http://repositorio.unicamp.br/jspui/handle/REPOSIP/311138.
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Orientador: Maria Yolanda MakuchTese (doutorado) - Universidade Estadual de Campinas, Faculdade de Ciencias Medicas
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Resumo: Objetivo: Descrever as vivências de homens e mulheres ao estarem iniciando os procedimentos de fertilização in vitro (FIV) e em longo prazo após o fracasso da terapêutica. Metodologia: Para cumprir com os objetivos propostos foram feitos dois estudos qualitativos, ambos realizados no Ambulatório de Reprodução Humana do Departamento de Tocoginecologia da Faculdade de Ciências Médicas (UNICAMP). Para o primeiro estudo foi escolhida uma amostra proposital por variedade de tipos - casais sem filhos, com filhos e que a mulher tinha filhos de um relacionamento anterior - que tinham realizado a FIV sem sucesso quatro a sete anos antes de serem entrevistados. O número de participantes foi definido conforme o critério de saturação da informação. Foram conduzidas entrevistas semi-estruturadas utilizando-se um roteiro temático, as quais foram gravadas e transcritas. Para o segundo estudo foi realizada uma re-análise das entrevistas de casais sem filhos realizadas quatro a seis anos após o fracasso da FIV do estudo acima e de casais sem filhos, que estavam iniciando pela primeira vez ou repetindo os procedimentos da FIV de outro estudo. O número de entrevistas analisadas foi definido conforme o critério de saturação da informação. Resultados: No primeiro estudo, homens e mulheres consideraram a FIV como uma experiência válida, apesar de alguns terem vivenciado dificuldades no relacionamento conjugal após o fracasso, as quais foram sendo superadas em longo prazo. Em relação às etapas do procedimento, alguns participantes se referiram ao desenvolvimento folicular como uma etapa difícil e dolorida e alguns acreditavam que a medicação tinha causado mudanças permanentes no corpo das mulheres. Os homens referiram a etapa da coleta do sêmen como uma experiência desconfortável. Após o fracasso da FIV, a maioria dos casais considerou a possibilidade da adoção, porém poucos adotaram e outros participantes se dedicaram a novos projetos de vida que não fosse o de terem um filho. Casais sem filhos decidiram realizar novas tentativas de FIV em outros serviços devido a necessidade de continuarem tentando. No segundo estudo, ao se contrapor as vivências de homens e mulheres, iniciando os procedimentos e em longo prazo, os participantes falaram que a decisão tinha sido baseada principalmente na certeza de sucesso e algumas mulheres mencionaram a necessidade de serem iguais às "mulheres férteis". Nas entrevistas dos homens, tanto no início do procedimento como em longo prazo, foi observado que a decisão tinha sido mais influenciada pelo desejo das parceiras pela gravidez. Em relação às etapas da FIV, as mulheres que estavam iniciando o procedimento, referiram as injeções de hormônios como preocupante, e em longo prazo, as consideraram doloridas e algumas, semelhante ao resultado do primeiro estudo, também acreditavam que a medicação tinha causado mudanças permanentes em seus corpos. Os homens, em geral, falaram da preocupação com a possível repercussão dos medicamentos na saúde da parceira. Algumas mulheres, tanto iniciando como em longo prazo, tinham a percepção de que a coleta do sêmen não tinha incomodado os parceiros. A mesma percepção foi mencionada pela maioria dos homens iniciando os procedimentos, embora a lembrança que prevaleceu em longo prazo foi a de ter sido uma experiência tensa e constrangedora. Conclusão: Homens e mulheres, devido à esperança pela gravidez, tendem a não se incomodarem com as exigências dos procedimentos, o que não ocorreu após o fracasso da FIV. Em longo prazo, homens e mulheres consideraram os procedimentos da FIV como uma experiência positiva e foram capazes de se dedicarem a outros projetos de vida, os quais foram considerados satisfatórios, exceto para os casais sem filhos.
Abstract: Objective: To describe the life experience of men and women at the beginning and after unsuccessful in vitro fertilization (IVF) procedures. Methods: Two qualitative studies were conducted to achieve the proposed aims, at the Department of Obstetrics and Gynecology, School of Medical Sciences, University of Campinas (UNICAMP). Participants for the first study were chosen using a purposive sampling per variety of types - couples without children, couples who already had children and wished to have another child, and couples in which the women had children from a previous relationship - that had experienced unsuccessful IVF four to seven years before the interviews. The number of interviews carried out was defined according to the criteria of information saturation. Semi-structured interviews were conducted using a thematic script, which were tape-recorded and transcribed. The second study was based on the re-analysis of the interviews of couples without children interviewed four to six years after an unsuccessful IVF from the study above and couples without children interviewed at the beginning of the procedures the first time they were participating or when repeating IVF from a previously conducted study. The number of interviews carried out was defined according to the criteria of information saturation. Results: In the first study, men and women considered IVF a positive experience, even though some couples recalled difficulties in their relationship after unsuccessful IVF, however they felt that their marital relationship had not been permanently affected. Regarding the stages of the procedure, some participants recalled follicular development as a difficult and painful stage in the treatment and some believed that the medication used during that phase had resulted in body changes, in some cases considered permanent. The men referred to semen collection as an uncomfortable experience. The majority of the couples had considered adoption, but only a few couples actually adopted and others participants had focused on new life projects other than having children. Couples who had no children recalled they had pursued treatment in other clinics due to the necessity to keep trying. In the second study, comparing men and women's life experiences at the beginning of the procedures and in the long-term, all participants recalled that their decision to undergo IVF had been based principally on the certainty of success, and some women referred to their need to be like "a fertile women". In men's interviews, at the beginning of procedures and in the long-term, it was observed that the decision to participate in IVF procedures had been mainly based on woman's desire of pregnancy. Regarding the stages of the procedures, women who were beginning the procedures referred the hormone injections as preoccupying and in the long term they remembered as painful stage and some, similar to the results of the first study, believed that had resulted in permanent body changes. Men, in general, referred to worries about the possible repercussion of the medicines used during this stage in their partner's health. Some women in the beginning and in long-term, had the perception that semen collection was not a stressful experience for the partners. A similar perception was mentioned by the majority of men beginning the procedures, although in the long term their recollection was of a stressful and embarrassing experience. Conclusion: Men and women due the hope for the pregnancy tend not to be bothered with the requirements of the procedures, different of what happened after IVF failure. In long-term, men and women, considered IVF procedures as a positive experience and were able to focus on other life projects, which were considered satisfactory, except for the couples without child.
Doutorado
Ciencias Biomedicas
Doutor em Tocoginecologia
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Botha, Barend HJ. "Systematic review: Availability, effectiveness and safety of assisted reproductive techniques in Sub-Saharan Africa." Master's thesis, University of Cape Town, 2018. http://hdl.handle.net/11427/29315.
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STUDY QUESTION: What is the evidence pertaining to availability, effectiveness and safety of assisted reproductive technology (ART) in sub-Saharan Africa?
SUMMARY ANSWER: According to overall limited and heterogeneous evidence, availability and utilization of ART are very low, clinical pregnancy rates largely compare to other regions but are accompanied by high multiple pregnancy rates, and in the near absence of data on deliveries and live births the true degree of effectiveness and safety remains to be established.
WHAT IS KNOWN ALREADY: In most world regions, availability, utilization and outcomes of ART are monitored and reported by national and regional ART registries. In sub-Saharan Africa there is only one national and no regional registry to date, raising the question what other evidence exists documenting the status of ART in this region.
STUDY DESIGN, SIZE, DURATION: A systematic review was conducted searching PUBMED, SCOPUS, AFRICAWIDE, WEB OF SCIENCE and CINAHL databases from January 2000 to June 2017. A total of 29 studies were included in the review. The extracted data were not suitable for meta-analysis.
PARTICIPANTS/MATERIALS, SETTING, METHOD: The review was conducted according to PRISMA guidelines. All peer-reviewed manuscripts irrespective of language or study design that presented original data pertaining to availability, effectiveness and safety of ART in sub-Saharan Africa were eligible for inclusion. Selection criteria were specified prior to the search. Two authors independently reviewed studies for possible inclusion and critically appraised selected manuscripts. Data were analyzed descriptively, being unsuitable for statistical analysis.
MAIN RESULTS AND THE ROLE OF CHANCE: The search yielded 810 references of which 29 were included based on the predefined selection and eligibility criteria. Extracted data came from 23 single centre observational studies, 2 global ART reports, 2 reviews, 1 national data registry and 1 community-based study. ART services were available in 10 countries and delivered by 80 centres in 6 of these. Data pertaining to number of procedures existed from 3 countries totalling 4619 fresh non-donor aspirations in 2010. The most prominent barrier to access was cost. Clinical pregnancy rates ranged between 21.2% to 43.9% per embryo transfer but information on deliveries and live births were lacking, seriously limiting evaluation of ART effectiveness. When documented, the rate of multiple pregnancy was high with information on outcomes similarly lacking.
LIMITATIONS, REASONS FOR CAUTION: The findings in this review are based on limited data from a limited number of countries, and are derived from heterogeneous studies, both in terms of study design and quality, many of which include small sample sizes. Although representing best available evidence, this requires careful interpretation regarding the degree of representativeness of the current status of ART in sub-Saharan Africa.
WIDER IMPLICATIONS OF THE FINDINGS: The true extent and outcome of ART in sub-Saharan Africa could not be reliably documented as the relevant information was not available. Current efforts are underway to establish a regional ART data registry in order to report and monitor availability, effectiveness and safety of ART thus contributing to evidence-based practice and possible development strategies.
STUDY FUNDING/COMPETING INTEREST(S): No funding was received for this study. The authors had no competing interests.
TRIAL REGISTRATION NUMBER: PROSPERO CRD42016032336
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Tomlins, Andrew Mark. "The analysis of male reproductive tract secretions and cells by modern nuclear magnetic resonance spectroscopic techniques." Thesis, Birkbeck (University of London), 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.263267.
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Tinnerholm, Ljungberg Helena. "Omöjliga familjen : Ideologi och fantasi i svensk reproduktionspolitik." Doctoral thesis, Stockholms universitet, Statsvetenskapliga institutionen, 2015. http://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-122219.
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The relationship between the state and the people is a central theme in political theory. Discussions in this field have often centered on how a people can come to constitute a state. Less attention, however, has been directed toward the state’s role in constituting and recreating its people. This book examines the Swedish state’s role in forming the people by regulating the use of reproductive techniques: insemination, in vitro fertilization (IVF), and donations of sperm and eggs. The study focuses on how the issue of assisted reproduction was handled and problematized in Swedish policymaking between 1981 and 2005. What problem representations dominated the political debates and decision-making processes surrounding assisted reproduction? How was conflict expressed within the field of reproductive politics (i.e., what aspects caused conflict or political disagreement)? How did collective fantasies play into the political treatment of reproductive technologies? Using historical government and Riksdag material, four major policy debates have been analyzed, from the first legal regulation of assisted reproduction in Sweden in the 1980s up until the inclusion of lesbian couples as beneficiaries of gamete donation. Theoretically, the study is inspired by Ernesto Laclau and Chantal Mouffe’s political discourse theory, Lacanian psychoanalysis, and the “logics approach” developed by Jason Glynos and David Howarth. This combination of perspectives allows for a dual focus on both the form of political articulations and their affective force. Thus, the analysis tries to capture what was taken for granted within the discourse on reproduction (social logics), what arose as points of political conflict or contention (political logics), as well as the affective underpinnings of these social constructions and struggles (fantasmatic logics). The main result of the study is that even though the period saw a quite revolutionary development of new reproductive technologies, the reproduction policies under study took on much more moderate and hesitant character. Throughout the analyzed period there was a more or less consensual view that new reproductive technologies should only be allowed if they did not go against the “child’s best interest.” At the same time, there was significant political conflict over what constituted this interest. Moreover, the reforms that were made never fully embraced the radical implications of the new technologies. Rather, they clung on to previously established patterns of what a “real” family looked like. Thus, every move to allow a new technology or include another category of people as legitimate users of that technology was contingent upon the articulation of a discursive equivalence with previously naturalized methods of reproduction, ultimately taking the heterosexual, nuclear family as an implicit model. Finally, I argue that the production of “sense” in this terrain of radical undecidability was dependent on the mobilization of a series of collective fantasies about “natural life processes,” “nature’s imperfections,” “a humanist view of mankind,” “the stable, original nuclear family”, and so on.
Books on the topic "Reproductive Techniques"
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P, Marrs Richard, ed. Assisted reproductive technologies. Boston: Blackwell Scientific Publications, 1993.
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K, Gardner David, ed. Textbook of assisted reproductive techniques: Laboratory and clinical perspectives. 2nd ed. London: Taylor & Francis, 2004.
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K, Gardner David, ed. Textbook of assisted reproductive techyniques: Laboratory and clinical perspectives. 2nd ed. London: Martin Dunitz ; Boca Raton, FL, 2004.
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Gardner, David K., Ariel Weissman, Colin M. Howles, and Zeev Shoham. Textbook of Assisted Reproductive Techniques. 6th ed. Boca Raton: CRC Press, 2023. http://dx.doi.org/10.1201/9781003268598.
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Gardner, David K., Ariel Weissman, Colin M. Howles, and Zeev Shoham. Textbook of Assisted Reproductive Techniques. 6th ed. Boca Raton: CRC Press, 2023. http://dx.doi.org/10.1201/9781003268611.
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Cusine, Douglas J. New reproductive techniques: A legal perspective. Aldershot, Hants, England: Gower, 1988.
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Guller, Seth, Carlo Bulletti, and D. de Ziegler. Reproductive science. Edited by New York Academy of Sciences. Boston, Mass: Published by Blackwell Pub. on behalf of the New York Academy of Sciences, 2011.
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R, Carr Bruce, and Blackwell Richard E, eds. Textbook of reproductive medicine. 2nd ed. Stamford, Conn: Appleton & Lange, 1998.
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R, Carr Bruce, and Blackwell Richard E, eds. Textbook of reproductive medicine. Norwalk, Conn: Appleton & Lange, 1993.
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World, Congress on Human Reproduction (10th 1999 Salvador Brazil). Reproductive medicine: A millennium review : the proceedings of the 10th World Congress on Human Reproduction. Pearl River, NY: Parthenon Pub. Group, 1999.
Find full textBook chapters on the topic "Reproductive Techniques"
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Huang, Jack Yu Jen, and Zev Rosenwaks. "Assisted Reproductive Techniques." In Methods in Molecular Biology, 171–231. New York, NY: Springer New York, 2014. http://dx.doi.org/10.1007/978-1-4939-0659-8_8.
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Castresana, Javier S., and Paula Lázcoz. "Techniques for DNA Analysis." In Reproductive Endocrinology, 83–96. Boston, MA: Springer US, 2009. http://dx.doi.org/10.1007/978-0-387-88186-7_8.
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Turek, Paul J. "Sperm Retrieval Techniques." In Reproductive Endocrinology and Infertility, 453–65. New York, NY: Springer New York, 2010. http://dx.doi.org/10.1007/978-1-4419-1436-1_29.
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Jackson, Katharine V., and Catherine Racowsky. "Embryo Culture Techniques." In Reproductive Endocrinology and Infertility, 613–32. New York, NY: Springer New York, 2010. http://dx.doi.org/10.1007/978-1-4419-1436-1_41.
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Patton, Elizabeth W., and Magdy Milad. "Techniques in Reproductive Surgery." In Atlas of Single-Port, Laparoscopic, and Robotic Surgery, 95–103. New York, NY: Springer New York, 2014. http://dx.doi.org/10.1007/978-1-4614-6840-0_8.
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Sel, Görker. "Artificial Reproductive Techniques (ART)." In Practical Guide to Oral Exams in Obstetrics and Gynecology, 275–78. Cham: Springer International Publishing, 2019. http://dx.doi.org/10.1007/978-3-030-29669-8_43.
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Garg, Nisha, Elizabeth W. Patton, and Magdy P. Milad. "Techniques in Reproductive Surgery." In Atlas of Robotic, Conventional, and Single-Port Laparoscopy, 111–19. Cham: Springer International Publishing, 2022. http://dx.doi.org/10.1007/978-3-030-93213-8_9.
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Alkolombre, Patricia. "Psychoanalysis and Reproductive Techniques." In The Desire and Passion for a Child, 105–32. London: Routledge, 2023. http://dx.doi.org/10.4324/9781003296713-7.
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Campos-Galindo, Inmaculada. "Cytogenetics techniques." In Human Reproductive Genetics, 33–48. Elsevier, 2020. http://dx.doi.org/10.1016/b978-0-12-816561-4.00003-x.
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"Advanced reproductive techniques." In Equine reproductive physiology, breeding and stud management, 389–97. Wallingford: CABI, 2020. http://dx.doi.org/10.1079/9781789242232.0389.
Full textConference papers on the topic "Reproductive Techniques"
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Geethamalini, S., S. Balamuralitharan, M. Radha, V. Geetha, and A. Rathinasamy. "Stability analysis of deterministic SEIA worm model by reproductive number." In THE 11TH NATIONAL CONFERENCE ON MATHEMATICAL TECHNIQUES AND APPLICATIONS. AIP Publishing, 2019. http://dx.doi.org/10.1063/1.5112229.
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Bajkovec, Lucija, Ines Begović, Ana-Marija Đaković, Milan Milošević, Urelija Rodin, and Aida Mujkić. "475 Assisted reproductive technology techniques and risk for neurodevelopmental disorders." In 10th Europaediatrics Congress, Zagreb, Croatia, 7–9 October 2021. BMJ Publishing Group Ltd and Royal College of Paediatrics and Child Health, 2021. http://dx.doi.org/10.1136/archdischild-2021-europaediatrics.475.
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Ilina, I. V., M. A. Filatov, Y. V. Khramova, and D. S. Sitnikov. "Embryo microsurgery with femtosecond laser: novel techniques for assisted reproductive technologies." In 2020 International Conference Laser Optics (ICLO). IEEE, 2020. http://dx.doi.org/10.1109/iclo48556.2020.9285872.
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Mustafayeva, Ilhama. "#975 Physical and sexual development indicators in pubertal girls born with assisted reproductive techniques." In ESGO 2023 Congress. BMJ Publishing Group Ltd, 2023. http://dx.doi.org/10.1136/ijgc-2023-esgo.440.
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Ilina, Inna V., Yulia V. Khramova, Anna D. Ivanova, Maxim A. Filatov, and Dmitry S. Sitnikov. "Novel techniques for embryo microsurgery based on femtosecond lasers for applications in assisted reproductive technologies." In Translational Biophotonics: Diagnostics and Therapeutics, edited by Lothar D. Lilge and Zhiwei Huang. SPIE, 2021. http://dx.doi.org/10.1117/12.2614810.
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Malanchuk, Larysa, Mariia Riabokon, Artem Malanchuk, Serhiy Malanchuk, Svitlana Riabokon, and Olha Kovalchuk. "The Use of Data Mining Techniques for Analysis of Menstrual Cycle Parameters and Prognosis of Migraine Symptoms in Reproductive Age Women." In 2020 10th International Conference on Advanced Computer Information Technologies (ACIT). IEEE, 2020. http://dx.doi.org/10.1109/acit49673.2020.9208988.
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Scherr, Thomas F., Gerald Knapp, Terrence Tiersch, W. Todd Monroe, and Krishnaswamy Nandakumar. "The Activation of Zebrafish Sperm Cells in a Micromixer." In ASME 2013 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2013. http://dx.doi.org/10.1115/sbc2013-14734.
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The freshwater fish, Danio rerio (zebrafish), have become widely used as a model organism for vertebrate development, DNA mutation, and human disease studies [1]. Maintaining live colonies of the numerous developed strains of zebrafish under investigation can be prohibitively costly. As such, there is a growing need to catalog their reproductive cells and have them available on demand [2]. Thus cryopreservation of model strain gametes has become an important endeavor, where evaluation of freezing and thawing techniques is currently a bottleneck to these procedures.
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Canti, V., L. Cavallo, R. De Lorenzo, A. Calligaro, F. Ruffilli, M. Tonello, P. Melissa, et al. "AB1509 MATERNAL-FETAL OUTCOMES OF PREGNANT WOMEN WITH SYSTEMIC AUTOIMMUNE DISEASES: EFFICACY AND SAFETY OF ASSISTED REPRODUCTIVE TECHNIQUES IN THREE TERTIARY CARE CENTER IN ITALY." In EULAR 2024 European Congress of Rheumatology, 12-15 June. Vienna, Austria. BMJ Publishing Group Ltd and European League Against Rheumatism, 2024. http://dx.doi.org/10.1136/annrheumdis-2024-eular.2337.
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Caraba, Ion Valeriu, Marioara Nicoleta Caraba, Gabi Dumitrescu, Elena Pet, and Roxana Popescu. "OPTIMIZING A PROTOCOL FOR INDUCING AND SYNCHRONIZING ESTRUS IN SHEEP DURING NON-BREEDING SEASON." In 23rd SGEM International Multidisciplinary Scientific GeoConference 2023. STEF92 Technology, 2023. http://dx.doi.org/10.5593/sgem2023v/6.2/s25.37.
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Induction and synchronization of estrus during non-breeding season is the most frequently used biotechnological method of assisted reproduction in sheep. Synchronization of estrus in sheep is a very important breeding technique as it can effectively improve the reproductive performance of sheep, minimize the effects of seasonal factors on sheep reproduction, extend the breeding season of sheep, shorten the estrus interval, increase the gestation rate, increase the birth rate, it can also lead to the shortening of the time between generations. The study proposes a method of inducing and synchronizing estrus during non-breeding season in Turcan sheep, which uses vaginal sponges with FGA (20 mg), supplemented by the administration of PGF2? (0.20 mg) and GnRH (7 �g). The analyzed parameters were: sponge loss rate, vaginitis onset rate, total percentage of ewes in estrus and gestation rate. The results obtained for the induction rate and synchronization of estrus during non-breeding, the reproductive period in sheep from the Turcana breed of 88.88%, respectively for the rate of pregnancy deception of 81.25% indicate that the method used was an efficient one.
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Nagy, Zsófia. "Comparison of Surrogacy laws of Austria, Slovakia and Ukraine." In Naděje právní vědy 2022. University of West Bohemia, Czech Republic, 2023. http://dx.doi.org/10.24132/zcu.nadeje.2022.421-431.
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Assisted reproductive technology has introduced a large scope of medical solutions for infertile couples to found a family, however surrogacy seems to be the most debated, as it triggers many ethical and legal questions. The multicolor of legal reactions to this sensitive issue can be represented through the legislation of the three countries in the Central European region, despite the decision making ot the ECtHR and the continuously ongoing europeanization and unification of law in Europe. However, in this regard, the domestic legislation enjoys prime attention and significance, because the ECtHR approaches these cases with a relatively ,,open-mind“ by giving the member state a large margin of appreciation in their legislation of delicate topics. This carefulness of the ECtHR was represented in case of Austria, where it highlighted the importance of free discretion of the country to decide whether to constrain surrogacy or not. Austria with this validation could maintain its prohibitive legislation towards surrogacy, but could also permit certain assisted reproductive techniques. From the Slovak domestic legislation we can demonstrate how certain countries can take the path of the complete non-regulation of surrogacy methods. The lack of detailed regulation on ART, the implicit ,,ban“ on surrogacy arrangements, and the determination of motherhood on gestational basis all reflect and contribute to the conservative approach Slovakia generally demonstartes in the field of reproductive and sexual rights. One prime example of permitting legislation on surrogacy comes from Ukraine, where even commercial surrogacy has been acceptable since the 1990s. There, the domestic legislation tries to protect all the three subjects of the surrogacy arrangement, also lessens the complications when issuing the birth certificate of the child. Despite the complex legislation of ART, some key features and concepts are not taken into consideration in these laws, which may endanger the legal certainty of the parties. By examining all the three legal approaches (prohibition, permission, non-regulation) a state can chose in regulating surrogacy through the examples of Austria, Slovakia and Ukraine, we can conclude that neither of them may seem satisfactory. The lack of an European standard in this regard may cause serious inadequacies, on the other hand one shall respect the ethical and moral reasoning of the state when drafting a regulation on such a delicate issue. However, this free attitude may not be maintainable in the future, especially with the strong emergence of international surrogacy cases.
Reports on the topic "Reproductive Techniques"
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Heifetz, Yael, and Michael Bender. Success and failure in insect fertilization and reproduction - the role of the female accessory glands. United States Department of Agriculture, December 2006. http://dx.doi.org/10.32747/2006.7695586.bard.
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The research problem. Understanding of insect reproduction has been critical to the design of insect pest control strategies including disruptions of mate-finding, courtship and sperm transfer by male insects. It is well known that males transfer proteins to females during mating that profoundly affect female reproductive physiology, but little is known about the molecular basis of female mating response and no attempts have yet been made to interfere with female post-mating responses that directly bear on the efficacy of fertilization. The female reproductive tract provides a crucial environment for the events of fertilization yet thus far those events and the role of the female tract in influencing them are poorly understood. For this project, we have chosen to focus on the lower reproductive tract because it is the site of two processes critical to reproduction: sperm management (storage, maintenance, and release from storage) and fertilization. E,fforts during this project period centered on the elucidation of mating responses in the female lower reproductive tract The central goals of this project were: 1. To identify mating-responsive genes in the female lower reproductive tract using DNA microarray technology. 2. In parallel, to identify mating-responsive genes in these tissues using proteomic assays (2D gels and LC-MS/MS techniques). 3. To integrate proteomic and genomic analyses of reproductive tract gene expression to identify significant genes for functional analysis. Our main achievements were: 1. Identification of mating-responsive genes in the female lower reproductive tract. We identified 539 mating-responsive genes using genomic and proteomic approaches. This analysis revealed a shift from gene silencing to gene activation soon after mating and a peak in differential gene expression at 6 hours post-mating. In addition, comparison of the two datasets revealed an expression pattern consistent with the model that important reproductive proteins are pre-programmed for synthesis prior to mating. This work was published in Mack et al. (2006). Validation experiments using real-time PCR techniques suggest that microarray assays provide a conservativestimate of the true transcriptional activity in reproductive tissues. 2.lntegration of proteomics and genomics data sets. We compared the expression profiles from DNA microarray data with the proteins identified in our proteomic experiments. Although comparing the two data sets poses analyical challenges, it provides a more complete view of gene expression as well as insights into how specific genes may be regulated. This work was published in Mack et al. (2006). 3. Development of primary reproductive tract cell cultures. We developed primary cell cultures of dispersed reproductive tract cell types and determined conditions for organ culture of the entire reproductive tract. This work will allow us to rapidly screen mating-responsive genes for a variety of reproductive-tract specifi c functions. Scientific and agricultural significance. Together, these studies have defined the genetic response to mating in a part of the female reproductive tract that is critical for successful fertllization and have identified alarge set of mating-responsive genes. This work is the first to combine both genomic and proteomic approaches in determining female mating response in these tissues and has provided important insights into insect reproductive behavior.
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Yang, Liu, FX Liang, Y. Yuan, LY Wang, XF Luo, XH Zhang, and L. Yao. Efficacy of progestin-primed ovarian stimulation (PPOS) in patients with polycystic ovary syndrome during IVF/ICSI treatments: A systematic review and meta-analysis. INPLASY - International Platform of Registered Systematic Review and Meta-analysis Protocols, April 2023. http://dx.doi.org/10.37766/inplasy2023.4.0059.
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Review question / Objective: This systematic review aims to evaluate the efficacy of progestin-primed ovarian stimulation (PPOS) on reproductive outcomes in patients with polycystic ovary syndrome (PCOS) undergoing assisted reproductive techniques. Condition being studied: PCOS is a common endocrine disorder that can cause infertility in women of childbearing age. The PPOS protocol, which involves oral progestins with gonadotropin (Gn), has been shown to be effective and safe in treating patients with PCOS. However, the question of whether PPOS provides a significant benefit over conventional GnRH analogue protocols in PCOS patients is still controversial.
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Moore, Gloria A., Gozal Ben-Hayyim, Charles L. Guy, and Doron Holland. Mapping Quantitative Trait Loci in the Woody Perennial Plant Genus Citrus. United States Department of Agriculture, May 1995. http://dx.doi.org/10.32747/1995.7570565.bard.
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As is true for all crops, production of Citrus fruit is limited by traits whose characteristics are the products of many genes (i.e. cold hardiness). In order to modify these traits by marker aided selection or molecular genetic techniques, it is first necessary to map the relevant genes. Mapping of quantitative trait loci (QTLs) in perennial plants has been extremely difficult, requiring large numbers of mature plants. Production of suitable mapping populations has been inhibited by aspects of reproductive biology (e.g. incompatibility, apomixis) and delayed by juvenility. New approaches promise to overcome some of these obstacles. The overall objective of this project was to determine whether QTLs for environmental stress tolerance could be effectively mapped in the perennial crop Citrus, using an extensive linkage map consisting of various types of molecular markers. Specific objectives were to: 1) Produce a highly saturated genetic linkage map of Citrus by continuing to place molecular markers of several types on the map. 2) Exploiting recently developed technology and already characterized parental types, determine whether QTLs governing cold acclimation can be mapped using very young seedling populations. 3) Determine whether the same strategy can be transferred to a different situation by mapping QTLs influencing Na+ and C1- exclusion (likely components of salinity tolerance) in the already characterized cross and in new alternative crosses. 4) Construct a YAC library of the citrus genome for future mapping and cloning.
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Delwiche, Michael, Boaz Zion, Robert BonDurant, Judith Rishpon, Ephraim Maltz, and Miriam Rosenberg. Biosensors for On-Line Measurement of Reproductive Hormones and Milk Proteins to Improve Dairy Herd Management. United States Department of Agriculture, February 2001. http://dx.doi.org/10.32747/2001.7573998.bard.
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The original objectives of this research project were to: (1) develop immunoassays, photometric sensors, and electrochemical sensors for real-time measurement of progesterone and estradiol in milk, (2) develop biosensors for measurement of caseins in milk, and (3) integrate and adapt these sensor technologies to create an automated electronic sensing system for operation in dairy parlors during milking. The overall direction of research was not changed, although the work was expanded to include other milk components such as urea and lactose. A second generation biosensor for on-line measurement of bovine progesterone was designed and tested. Anti-progesterone antibody was coated on small disks of nitrocellulose membrane, which were inserted in the reaction chamber prior to testing, and a real-time assay was developed. The biosensor was designed using micropumps and valves under computer control, and assayed fluid volumes on the order of 1 ml. An automated sampler was designed to draw a test volume of milk from the long milk tube using a 4-way pinch valve. The system could execute a measurement cycle in about 10 min. Progesterone could be measured at concentrations low enough to distinguish luteal-phase from follicular-phase cows. The potential of the sensor to detect actual ovulatory events was compared with standard methods of estrus detection, including human observation and an activity monitor. The biosensor correctly identified all ovulatory events during its testperiod, but the variability at low progesterone concentrations triggered some false positives. Direct on-line measurement and intelligent interpretation of reproductive hormone profiles offers the potential for substantial improvement in reproductive management. A simple potentiometric method for measurement of milk protein was developed and tested. The method was based on the fact that proteins bind iodine. When proteins are added to a solution of the redox couple iodine/iodide (I-I2), the concentration of free iodine is changed and, as a consequence, the potential between two electrodes immersed in the solution is changed. The method worked well with analytical casein solutions and accurately measured concentrations of analytical caseins added to fresh milk. When tested with actual milk samples, the correlation between the sensor readings and the reference lab results (of both total proteins and casein content) was inferior to that of analytical casein. A number of different technologies were explored for the analysis of milk urea, and a manometric technique was selected for the final design. In the new sensor, urea in the sample was hydrolyzed to ammonium and carbonate by the enzyme urease, and subsequent shaking of the sample with citric acid in a sealed cell allowed urea to be estimated as a change in partial pressure of carbon dioxide. The pressure change in the cell was measured with a miniature piezoresistive pressure sensor, and effects of background dissolved gases and vapor pressures were corrected for by repeating the measurement of pressure developed in the sample without the addition of urease. Results were accurate in the physiological range of milk, the assay was faster than the typical milking period, and no toxic reagents were required. A sampling device was designed and built to passively draw milk from the long milk tube in the parlor. An electrochemical sensor for lactose was developed starting with a three-cascaded-enzyme sensor, evolving into two enzymes and CO2[Fe (CN)6] as a mediator, and then into a microflow injection system using poly-osmium modified screen-printed electrodes. The sensor was designed to serve multiple milking positions, using a manifold valve, a sampling valve, and two pumps. Disposable screen-printed electrodes with enzymatic membranes were used. The sensor was optimized for electrode coating components, flow rate, pH, and sample size, and the results correlated well (r2= 0.967) with known lactose concentrations.
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Anglade, Boaz, and Julia Escobar. Effect of Violence against Women on Victims and their Children: Evidence from Central America, the Dominican Republic, and Haiti. Inter-American Development Bank, March 2021. http://dx.doi.org/10.18235/0003157.
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This paper presents a systematic overview of the evidence of violence against women in the Central America, Mexico, Panama, Haiti, and Dominican Republic region and examines its impact on the well-being of women and their children. Population-based surveys show that violence against women remains a widespread issue in the region. The proportion of women who have experienced physical or sexual violence at least once in their lifetime varies between 13% and 53%; Panama has the lowest rate while Mexico and El Salvador have the highest. The percentage of women who have experienced violence within private spheres ranges between 17% and 24%. Also, homicidal violence targeting women remains a major problem in the region. Using a novel propensity score reweighting technique, we assess the impact of violence on a series of outcome variables related to a womans health and socioeconomic condition. We find evidence that violence against women negatively affects victims reproductive and physical health as well as their fertility preferences. We also find evidence that violence against mothers has an adverse effect on childrens advancement in school and overall health.
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Epiphan, Jean, and Steven Handel. Trajectory of forest vegetation under contrasting stressors over a 26-year period, at Morristown National Historical Park: Focused condition assessment report. National Park Service, March 2023. http://dx.doi.org/10.36967/2297281.
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The Jockey Hollow section and the New Jersey Brigade Area of Morristown National Historical Park (MORR) are predominantly comprised of upland oak-hickory forests that have regrown over the past 200 years from previous land uses. The forest is being damaged by two major stressors, a large population of white-tailed deer and an abundance of non-native, invasive shrubs and herbaceous species. This study explores changes to the forest over 26 years and suggests management techniques to avoid future degradation. The forest is typical of many upland stands in the region, and studies here would be applicable to many lands controlled by the National Park Service and to many public and private land owners. In 1995, 18 vegetation experimental plots were established in the forest, each 20 x 20 m. Ten plots were in areas that had no non-native, invasive plants. The other eight plots had invasive species. All trees, shrubs and a sampling of herbs were recorded in each of the 18 plots. At that time, no GPS technology was available and handwritten maps were used to record locations. The plots were revisited and resurveyed in 2001; however, only 13 plots were found. This 2021 study is a new survey of the plot conditions. The investigators were able to relocate 17 of the original plots. New GPS locations were recorded for these 17 plots to facilitate future studies. The goal of the study was to test if changes over 26 years in forest conditions differed between the original invaded plots as compared to the uninvaded plots. Also, these data will allow us to measure the progress of invasion into previously uninvaded areas. Together, these results will allow the forest managers to focus attention on the most aggressive plant invaders and to understand the fate of this forest type that is being challenged by deer and non-native plants. Over the last 26 years there has been no hunting for deer here. Also, the plots were not within the few deer exclosures at MORR; deer were able to enter the landscape from surrounding heavily wooded areas. Data were collected in four layers of vegetation – mature trees, saplings, shrubs, and herbaceous groundcovers. The mature trees in the invaded forest plots demonstrated declining trends. The species richness declined by 6%, the average number of trees declined by 30%, white ash and flowering dogwood had the most losses, and basal area did not increase over time because very few new saplings grew into mature tree sizes. The uninvaded plots’ mature trees also revealed a 20% declined in richness, number of trees declined by 18%, the greatest losses occurred for red maple and black birch, but basal area increased slightly due to growth of large persisting trees. Saplings in the invaded forest experienced declines over the 26 years. Species richness declined by 38%, number of native saplings decreased by 44%, and number of invasive saplings increased by 600%. In the uninvaded forest, the conditions and trends were variable. Richness decreased by 21%, no invasive saplings found, number of native saplings increased by 37% (due to increases in American beech). With American beech excluded, the number of native saplings decreased by 60%. In both forest types, the declining number of native saplings was primarily caused by excessive deer damage. For the shrub layer in invaded plots, Japanese barberry stems increased by 122% by 2001 and 276% by 2021. Barberry became the dominant species. Similarly, wineberry stands increased 486% in 2001 and 157% for 2021. It is now the second most common species. However, in the uninvaded plots there was no significant increase in the number of barberry stems and wineberry was not present in 1995 or 2001, and only averaged 1.5 stems per plot in 2021. Neither species has a significant presence now and eradication is possible. A major finding is that the process of invasion of these shrubs over 26 years is very slow. For the herbaceous plants, in the invaded plots there was a sharp decrease in cover by 2021 due to the competitive impact by the abundant invasive shrubs. The invasive Japanese stiltgrass declined 86% in cover and native Carex (sedge) species declined by 78%. In the uninvaded forest plots, stiltgrass was present in very low amounts and did not increase significantly over 26 years. The number of quadrats with any stiltgrass only increased from 3 to 5 over the 26 years. These data show that stiltgrass invades slowly in the uninvaded plots, but in the invaded plots it was greatly replaced by invasive shrubs. Overall, the rate of change of the native herb cover was slow. Together, these data suggest that currently uninvaded forest areas can be protected by monitoring and rigorously eliminating any initial invasions by non-native shrubs and herbs. Once these species reach a significant presence in number of stems or cover in a plot they explode in number. Early intervention when invasive plants first appear is the most practical management tool. Reduction of deer density will also increase the reproductive potential and sustainability of our main forest tree species. Planting of new young canopy tree species, with protection from deer, can retard the ability of invasive plants to begin new populations.
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Yatsymirska, Mariya. MODERN MEDIA TEXT: POLITICAL NARRATIVES, MEANINGS AND SENSES, EMOTIONAL MARKERS. Ivan Franko National University of Lviv, February 2022. http://dx.doi.org/10.30970/vjo.2022.51.11411.
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The article examines modern media texts in the field of political journalism; the role of information narratives and emotional markers in media doctrine is clarified; verbal expression of rational meanings in the articles of famous Ukrainian analysts is shown. Popular theories of emotions in the process of cognition are considered, their relationship with the author’s personality, reader psychology and gonzo journalism is shown. Since the media text, in contrast to the text, is a product of social communication, the main narrative is information with the intention of influencing public opinion. Media text implies the presence of the author as a creator of meanings. In addition, media texts have universal features: word, sound, visuality (stills, photos, videos). They are traditionally divided into radio, TV, newspaper and Internet texts. The concepts of multimedia and hypertext are related to online texts. Web combinations, especially in political journalism, have intensified the interactive branching of nonlinear texts that cannot be published in traditional media. The Internet as a medium has created the conditions for the exchange of ideas in the most emotional way. Hence Gonzo’s interest in journalism, which expresses impressions of certain events in words and epithets, regardless of their stylistic affiliation. There are many such examples on social media in connection with the events surrounding the Wagnerians, the Poroshenko case, Russia’s new aggression against Ukraine, and others. Thus, the study of new features of media text in the context of modern political narratives and emotional markers is important in media research. The article focuses review of etymology, origin and features of using lexemes “cмисл (meaning)” and “сенс (sense)” in linguistic practice of Ukrainians results in the development of meanings and functional stylistic coloring in the usage of these units. Lexemes “cмисл (meaning)” and “сенс (sense)” are used as synonyms, but there are specific fields of meanings where they cannot be interchanged: lexeme “сенс (sense)” should be used when it comes to reasonable grounds for something, lexeme “cмисл (meaning)” should be used when it comes to notion, concept, understanding. Modern political texts are most prominent in genres such as interviews with politicians, political commentaries, analytical articles by media experts and journalists, political reviews, political portraits, political talk shows, and conversations about recent events, accompanied by effective emotional narratives. Etymologically, the concept of “narrative” is associated with the Latin adjective “gnarus” – expert. Speakers, philosophers, and literary critics considered narrative an “example of the human mind.” In modern media texts it is not only “story”, “explanation”, “message techniques”, “chronological reproduction of events”, but first of all the semantic load and what subjective meanings the author voices; it is a process of logical presentation of arguments (narration). The highly professional narrator uses narration as a “method of organizing discourse” around facts and impressions, impresses with his political erudition, extraordinary intelligence and creativity. Some of the above theses are reflected in the following illustrations from the Ukrainian media: “Culture outside politics” – a pro-Russian narrative…” (MP Gabibullayeva); “The next will be Russia – in the post-Soviet space is the Arab Spring…” (journalist Vitaly Portnikov); “In Russia, only the collapse of Ukraine will be perceived as success” (Pavel Klimkin); “Our army is fighting, hiding from the leadership” (Yuri Butusov).
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Ficht, Thomas, Gary Splitter, Menachem Banai, and Menachem Davidson. Characterization of B. Melinensis REV 1 Attenuated Mutants. United States Department of Agriculture, December 2000. http://dx.doi.org/10.32747/2000.7580667.bard.
Full textAbstract:
Brucella Mutagenesis (TAMU) The working hypothesis for this study was that survival of Brucella vaccines was directly related to their persistence in the host. This premise is based on previously published work detailing the survival of the currently employed vaccine strains S19 and Rev 1. The approach employed signature-tagged mutagenesis to construct mutants interrupted in individual genes, and the mouse model to identify mutants with attenuated virulence/survival. Intracellular survival in macrophages is the key to both reproductive disease in ruminants and reticuloendothelial disease observed in most other species. Therefore, the mouse model permitted selection of mutants of reduced intracellular survival that would limit their ability to cause reproductive disease in ruminants. Several classes of mutants were expected. Colonization/invasion requires gene products that enhance host-agent interaction or increase resistance to antibacterial activity in macrophages. The establishment of chronic infection requires gene products necessary for intracellular bacterial growth. Maintenance of chronic infection requires gene products that sustain a low-level metabolism during periods characterized little or no growth (1, 2). Of these mutants, the latter group was of greatest interest with regard to our originally stated premise. However, the results obtained do not necessarily support a simplistic model of vaccine efficacy, i.e., long-survival of vaccine strains provides better immunity. Our conclusion can only be that optimal vaccines will only be developed with a thorough understanding of host agent interaction, and will be preferable to the use of fortuitous isolates of unknown genetic background. Each mutant could be distinguished from among a group of mutants by PCR amplification of the signature tag (5). This approach permitted infection of mice with pools of different mutants (including the parental wild-type as a control) and identified 40 mutants with apparently defective survival characteristics that were tentatively assigned to three distinct classes or groups. Group I (n=13) contained organisms that exhibited reduced survival at two weeks post-infection. Organisms in this group were recovered at normal levels by eight weeks and were not studied further, since they may persist in the host. Group II (n=11) contained organisms that were reduced by 2 weeks post infection and remained at reduced levels at eight weeks post-infection. Group III (n=16) contained mutants that were normal at two weeks, but recovered at reduced levels at eight weeks. A subset of these mutants (n= 15) was confirmed to be attenuated in mixed infections (1:1) with the parental wild-type. One of these mutants was eliminated from consideration due to a reduced growth rate in vitro that may account for its apparent growth defect in the mouse model. Although the original plan involved construction of the mutant bank in B. melitensis Rev 1 the low transformability of this strain, prevented accumulation of the necessary number of mutants. In addition, the probability that Rev 1 already carries one genetic defect increases the likelihood that a second defect will severely compromise the survival of this organism. Once key genes have been identified, it is relatively easy to prepare the appropriate genetic constructs (knockouts) lacking these genes in B. melitensis Rev 1 or any other genetic background. The construction of "designer" vaccines is expected to improve immune protection resulting from minor sequence variation corresponding to geographically distinct isolates or to design vaccines for use in specific hosts. A.2 Mouse Model of Brucella Infection (UWISC) Interferon regulatory factor-1-deficient (IRF-1-/- mice have diverse immunodeficient phenotypes that are necessary for conferring proper immune protection to intracellular bacterial infection, such as a 90% reduction of CD8+ T cells, functionally impaired NK cells, as well as a deficiency in iNOS and IL-12p40 induction. Interestingly, IRF-1-/- mice infected with diverse Brucella abortus strains reacted differently in a death and survival manner depending on the dose of injection and the level of virulence. Notably, 50% of IRF-1-/- mice intraperitoneally infected with a sublethal dose in C57BL/6 mice, i.e., 5 x 105 CFU of virulent S2308 or the attenuated vaccine S19, died at 10 and 20 days post-infection, respectively. Interestingly, the same dose of RB51, an attenuated new vaccine strain, did not induce the death of IRF-1-/- mice for the 4 weeks of infection. IRF-1-/- mice infected with four more other genetically manipulated S2308 mutants at 5 x 105 CFU also reacted in a death or survival manner depending on the level of virulence. Splenic CFU from C57BL/6 mice infected with 5 x 105 CFU of S2308, S19, or RB51, as well as four different S2308 mutants supports the finding that reduced virulence correlates with survival Of IRF-1-/- mice. Therefore, these results suggest that IRF-1 regulation of multi-gene transcription plays a crucial role in controlling B. abortus infection, and IRF-1 mice could be used as an animal model to determine the degree of B. abortus virulence by examining death or survival. A3 Diagnostic Tests for Detection of B. melitensis Rev 1 (Kimron) In this project we developed an effective PCR tool that can distinguish between Rev1 field isolates and B. melitensis virulent field strains. This has allowed, for the first time, to monitor epidemiological outbreaks of Rev1 infection in vaccinated flocks and to clearly demonstrate horizontal transfer of the strain from vaccinated ewes to unvaccinated ones. Moreover, two human isolates were characterized as Rev1 isolates implying the risk of use of improperly controlled lots of the vaccine in the national campaign. Since atypical B. melitensis biotype 1 strains have been characterized in Israel, the PCR technique has unequivocally demonstrated that strain Rev1 has not diverted into a virulent mutant. In addition, we could demonstrate that very likely a new prototype biotype 1 strain has evolved in the Middle East compared to the classical strain 16M. All the Israeli field strains have been shown to differ from strain 16M in the PstI digestion profile of the omp2a gene sequence suggesting that the local strains were possibly developed as a separate branch of B. melitensis. Should this be confirmed these data suggest that the Rev1 vaccine may not be an optimal vaccine strain for the Israeli flocks as it shares the same omp2 PstI digestion profile as strain 16M.
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Yatsymirska, Mariya. SOCIAL EXPRESSION IN MULTIMEDIA TEXTS. Ivan Franko National University of Lviv, February 2021. http://dx.doi.org/10.30970/vjo.2021.49.11072.
Full textAbstract:
The article investigates functional techniques of extralinguistic expression in multimedia texts; the effectiveness of figurative expressions as a reaction to modern events in Ukraine and their influence on the formation of public opinion is shown. Publications of journalists, broadcasts of media resonators, experts, public figures, politicians, readers are analyzed. The language of the media plays a key role in shaping the worldview of the young political elite in the first place. The essence of each statement is a focused thought that reacts to events in the world or in one’s own country. The most popular platform for mass information and social interaction is, first of all, network journalism, which is characterized by mobility and unlimited time and space. Authors have complete freedom to express their views in direct language, including their own word formation. Phonetic, lexical, phraseological and stylistic means of speech create expression of the text. A figurative word, a good aphorism or proverb, a paraphrased expression, etc. enhance the effectiveness of a multimedia text. This is especially important for headlines that simultaneously inform and influence the views of millions of readers. Given the wide range of issues raised by the Internet as a medium, research in this area is interdisciplinary. The science of information, combining language and social communication, is at the forefront of global interactions. The Internet is an effective source of knowledge and a forum for free thought. Nonlinear texts (hypertexts) – «branching texts or texts that perform actions on request», multimedia texts change the principles of information collection, storage and dissemination, involving billions of readers in the discussion of global issues. Mastering the word is not an easy task if the author of the publication is not well-read, is not deep in the topic, does not know the psychology of the audience for which he writes. Therefore, the study of media broadcasting is an important component of the professional training of future journalists. The functions of the language of the media require the authors to make the right statements and convincing arguments in the text. Journalism education is not only knowledge of imperative and dispositive norms, but also apodictic ones. In practice, this means that there are rules in media creativity that are based on logical necessity. Apodicticity is the first sign of impressive language on the platform of print or electronic media. Social expression is a combination of creative abilities and linguistic competencies that a journalist realizes in his activity. Creative self-expression is realized in a set of many important factors in the media: the choice of topic, convincing arguments, logical presentation of ideas and deep philological education. Linguistic art, in contrast to painting, music, sculpture, accumulates all visual, auditory, tactile and empathic sensations in a universal sign – the word. The choice of the word for the reproduction of sensory and semantic meanings, its competent use in the appropriate context distinguishes the journalist-intellectual from other participants in forums, round tables, analytical or entertainment programs. Expressive speech in the media is a product of the intellect (ability to think) of all those who write on socio-political or economic topics. In the same plane with him – intelligence (awareness, prudence), the first sign of which (according to Ivan Ogienko) is a good knowledge of the language. Intellectual language is an important means of organizing a journalistic text. It, on the one hand, logically conveys the author’s thoughts, and on the other – encourages the reader to reflect and comprehend what is read. The richness of language is accumulated through continuous self-education and interesting communication. Studies of social expression as an important factor influencing the formation of public consciousness should open up new facets of rational and emotional media broadcasting; to trace physical and psychological reactions to communicative mimicry in the media. Speech mimicry as one of the methods of disguise is increasingly becoming a dangerous factor in manipulating the media. Mimicry is an unprincipled adaptation to the surrounding social conditions; one of the most famous examples of an animal characterized by mimicry (change of protective color and shape) is a chameleon. In a figurative sense, chameleons are called adaptive journalists. Observations show that mimicry in politics is to some extent a kind of game that, like every game, is always conditional and artificial.
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Midline survey results: Integrating adolescent livelihood activities within a reproductive health program for urban slum dwellers in India. Population Council, 2002. http://dx.doi.org/10.31899/rh2002.1008.
Full textAbstract:
The Population Council in collaboration with CARE India has been conducting an operations research (OR) study to investigate the impact of adding a livelihoods component to the Action for Slum Dwellers' Reproductive Health, Allahabad (ASRHA) Project in Uttar Pradesh run by CARE India. The ASRHA Project selected peer educators from the slums and trained them in providing reproductive health (RH) information, communication skills, and group formation techniques. After the peer educators completed training, they conducted group sessions about livelihoods and savings. RH sessions were held alongside vocational counseling sessions. Population Council staff provided vocational training courses, both in the slums where the girls reside and in the city of Allahabad. In addition to surveys conducted prior to and following the intervention, the study also includes a midline assessment that measures the experiences of girls six months after they completed the first round of vocational training courses, or one year after the baseline survey. The midline survey was conducted in the experimental slums only, and this project update presents its findings.