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Journal articles on the topic 'Resin purification'

Author: Grafiati

Published: 11 January 2025

Last updated: 28 July 2025

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1

Li, Tuo Ping, Su Hong Li, Wei Na Fu, et al. "Purification of Soyasaponin with Macroporous Resin." Advanced Materials Research 396-398 (November 2011): 1379–81. http://dx.doi.org/10.4028/www.scientific.net/amr.396-398.1379.

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The conditions for purification of soyasaponin by macroporous resin adsorption strategies were analyzed. The results showed that macroporous resin D3520 was a suitable resin for the purification of soyasaponin. Static adsorption assay showed that 20:1 (w/w) D3520/soyasaponin at 40 oC for 2h adsorption were optimal for soyasaponin purification. In the column chromatography, 0.5BV (bed volume)/h flow rate would be suitable to reach higher purity of near 90 % soyasaponin.

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2

Talpur, Marvi Kanwal. "Highly Selective Purification of Grewia asiatica Anthocyanin Based on Macroporous Resins." Pakistan Journal of Analytical & Environmental Chemistry 22, no. 1 (2021): 44–52. http://dx.doi.org/10.21743/pjaec/2021.06.06.

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In the present study, enactments and separation characteristics of five extensively utilized adsorbents for enhancement and purification of Grewia asiatica Anthocyanins (ACNs) extracts were analysed. Among five tested resins (AB-8, Sepabeads Sp-700, C18SPE Cartridge, Sepabeads Sp-207 and AB-80), AB-8 resin exhibited the best adsorbent ability for Grewia asiatica ACNs (84.24mg/mL resin). Results of static adsorption tests revealed that AB-8 resin selected for kinetics and isotherm experiments followed a pseudo 2nd order model along with Langmuir isotherm. In order to improve operational procedu

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3

Li, Di-Hua, Yan Wang, Yuan-Shan Lv, et al. "Preparative Purification of Liriodendrin fromSargentodoxa cuneataby Macroporous Resin." BioMed Research International 2015 (2015): 1–9. http://dx.doi.org/10.1155/2015/861256.

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The preparative purification of liriodendrin fromSargentodoxa cuneatausing macroporous resin combined with crystallization process was evaluated. The properties of adsorption/desorption of liriodendrin on eight macroporous resins were investigated systematically. X-5 resin was selected as the most suitable medium for liriodendrin purification. The adsorption of liriodendrin on X-5 resin fitted well with the pseudo-second-order kinetic model and Langmuir isotherm model. Dynamic adsorption/desorption tests were performed using a glass column packed with X-5 resin to optimize the separation proce

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Usui, Kenji, Shin-ichiro Yokota, Kazuya Iwata, and Yoshio Hamada. "Novel Purification Process for Amyloid Beta Peptide(1-40)." Processes 8, no. 4 (2020): 464. http://dx.doi.org/10.3390/pr8040464.

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Amyloid beta peptide (Aβ)-related studies require an adequate supply of purified Aβ peptide. However, Aβ peptides are “difficult sequences” to synthesize chemically, and low yields are common due to aggregation during purification. Here, we demonstrate an easier synthesis, deprotection, reduction, cleavage, and purification process for Aβ(1-40) using standard 9-fluorenylmethyloxycarbonyl (Fmoc)-protected amino acids and solid-phase peptide synthesis (SPPS) resin [HMBA (4-hydroxymethyl benzamide) resin] that provides higher yields of Aβ(1-40) than previous standard protocols. Furthermore, purif

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5

Bi, Yong Guang, and Yao Quan Tan. "Study on Macroporous Resin Separation and Purification of Total Flavonoids of Plantago Process." Advanced Materials Research 550-553 (July 2012): 987–92. http://dx.doi.org/10.4028/www.scientific.net/amr.550-553.987.

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To study the macroporous resin separation and purification of total flavonoids of Plantago optimum conditions, the static adsorption and desorption experiments by filtering out the best macroporous resin, and plantain extract for the assessment of total flavonoid content indicators in the single-factor test based on the orthogonal design, the amount of resin in the hole, eluent concentration, alcohol consumption and other factors for the study to optimize the AB-8 macroporous resin separation and purification of total flavonoids plantain best process conditions. The results show that: the effe

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Arthapersada, Reno Imam, Muhammad Kurniawan Adiputra, Indra P. Hakim, et al. "Optimasi Biaya dalam Proses Pemurnian Metanol untuk Mengurangi Resin sebagai Limbah Bahan Berbahaya dan Beracun di PT Kaltim Methanol Industri." Jurnal Rekayasa Proses 14, no. 2 (2020): 148. http://dx.doi.org/10.22146/jrekpros.59553.

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Purification process of raw methanol from its impurities to produce pure methanol at PT. Kaltim Methanol Industri (PT KMI) is carried out by several steps, including degassing, distillation, and adsorption. One of the impurities, tri methyl amine (TMA), could be removed by adding NaOH. Another method to remove TMA is conducted by adsorption process on ion exchange resin on the vessel called TMA catchpot. The TMA catchpot performance is very crucial in methanol purification process. Thus, monitoring and optimization are required to be performed regularly. Once the TMA catchpot resin has exhaust

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7

Khandelwal, Payal. "Resin Built for Large-Scale Purification." Genetic Engineering & Biotechnology News 38, no. 6 (2018): 18–19. http://dx.doi.org/10.1089/gen.38.06.07.

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8

Xu, Li Ping, Xin Wang, and Peng Cheng Yao. "Study on Technology of Isolation and Purification of Corn Germ Glutathione with Macroporous Resin." Advanced Materials Research 183-185 (January 2011): 306–9. http://dx.doi.org/10.4028/www.scientific.net/amr.183-185.306.

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Study on Macroporous resin purified glutathione of corn germ , determine the best conditions for separation and purification as follows: glutathione and resin volume ratio 4:1, flow rate 1 mL / min ,adsorption time 50 min, elution and resin volume ratio of 4:1, analysis time 120 min, recovery rate of glutathione78.95 %.

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9

Ma, Jian Wei, and Ya Rui Song. "Purification Raw Water by Magnetic Resin (MIEX)." Advanced Materials Research 726-731 (August 2013): 3185–88. http://dx.doi.org/10.4028/www.scientific.net/amr.726-731.3185.

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The objective of this research was to compare enhanced coagulation with anion exchange for removal of natural organic matter (NOM) and bromide. Treatment with a magnetic ion exchange resin (MIEX) was the primary focus of this study. The performance of the magnetic ion exchange resin,MIEX, in the treatment of raw water was investigated. MIEX can effectively remove UV-absorbing substances DOC. The removal of organic substances is accompanied by the elimination of other undesirable components, such as nitrogen and phosphorus. The optimal process parameters are at resin doses of 5-10 mL L1and cont

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10

Kartika, Ika Amalia, Oky Tresia Ordian Bernia, Illah Sailah, Tirto Prakoso, and Yohanes Aris Purwanto. "A binary solvent for the simultaneous Calophyllum oil-resin extraction and purification." Research in Agricultural Engineering 65, No. 2 (2019): 63–69. http://dx.doi.org/10.17221/30/2018-rae.

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Sustainable biodiesel production can be realised by the use of a low-cost feedstock, efficient energy and renewable raw materials. The simultaneous Calophyllum oil-resin extraction and its purification using a binary solvent (n-hexane mixed with alcohol) were examined to meet those aspects. The extraction conditions effect was investigated to determine the optimal oil yield and quality. n-Hexane mixed with alcohol was extracted and purified effectively the oil from Calophyllum seeds. The oil yield and its quality were mainly affected by the n-hexane-to-alcohol ratio. The oil yield enhanced as

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11

Jin, Xue Yuan, Hong Liu, and San Fu Zhu. "Optimization for Purification Technology of Platycodins by Macroreticular Resin." Advanced Materials Research 781-784 (September 2013): 852–55. http://dx.doi.org/10.4028/www.scientific.net/amr.781-784.852.

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In order to purify the platycodins from Platycodon grandiflorum, AB-8 macroporous resin was used to test. Based on single factor experiments, orthogonal test was used to optimum the purification conditions. Adsorption pH, adsorption temperature, adsorption time and platycodins concentration were as factors and adsorption capacity was as index. The results were as follows: adsorption pH 6.0, adsorption temperature 40°C, adsorption tim110min, platycodins concentration 2.0mg/mL were the optimization conditions.The adsorption quantity reached at 39.1mg/g. So AB-8 macroporous resin was a suitable r

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12

Zhen, Yuanhang, Tao Zhang, Bo Jiang, and Jingjing Chen. "Purification and Characterization of Resistant Dextrin." Foods 10, no. 1 (2021): 185. http://dx.doi.org/10.3390/foods10010185.

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In this study, an efficient method for the purification of resistant dextrin (RD) using membrane filtration and anion exchange resin decolorization was developed, then the purified RD was characterized. In the membrane filtration stage, suspended solids in RD were completely removed, and the resulting product had a negligible turbidity of 2.70 ± 0.18 NTU. Furthermore, approximately half of the pigments were removed. Static decolorization experiments revealed that the D285 anion exchange resin exhibited the best decolorization ratio (D%), 84.5 ± 2.03%, and recovery ratio (R%), 82.8 ± 1.41%, amo

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13

Alliot, C., N. Audouin, A. C. Bonraisin, et al. "82Sr purification procedure using Chelex-100 resin." Applied Radiation and Isotopes 74 (April 2013): 56–60. http://dx.doi.org/10.1016/j.apradiso.2012.10.020.

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14

Moura, Pedro A. S., and Carlos A. Grande. "Purification of ytterbium using a cationic resin." Results in Surfaces and Interfaces 19 (May 2025): 100550. https://doi.org/10.1016/j.rsurfi.2025.100550.

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15

Ferreira, Marcionila O., Elisa M.B.D. Sousa, and Camila G. Pereira. "Purification of Crude Glycerine Obtained from Transesterification of Cottonseed Oil." International Journal of Chemical Reactor Engineering 11, no. 1 (2013): 385–92. http://dx.doi.org/10.1515/ijcre-2012-0071.

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Abstract The objective of this study was to purify glycerine obtained from the transesterification of cottonseed oil, using ion exchange resins. The batch process used strong cation, weak anion and a mixture of its resins. The purified glycerine was characterized as to metal content, colour and glycerol content. The experiments were conducted using the resins individually and in series. In the experiments in series, there was a constant decrease in conductivity. Analyses without the use of charcoal showed that conductivity behaved similarly to that of treatment with charcoal. For conductivity

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16

Hebert, Mathieu, Emmanuel Serra, Eugène Vorobiev, and Houcine Mhemdi. "Isolation and Purification of Mustard Glucosinolates by Macroporous Anion-Exchange Resin: Process Optimization and Kinetics’ Modelling." Processes 10, no. 2 (2022): 191. http://dx.doi.org/10.3390/pr10020191.

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Glucosinolates (GSL) (β-thioglucoside-N-hydroxy sulfates) are rich-sulfur secondary metabolites raising potential biofumigation interest due to their biological properties. Sinigrin and gluconapin are the main glucosinolates present in brown mustard seeds (Brassica juncea). These glucosinolates are very suitable for the development of phytosanitary products due to their fungicidal, bactericidal and insecticidal effects. In this work, the purification of sinigrin and gluconapin extracted from defatted mustard seeds was studied using macroporous anion exchange resins. A strongly and a weakly ani

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17

Lu, Cheng, Jack Bevers, Tulika Tyagi, et al. "AviTrap: A novel solution to achieve complete biotinylation." PLOS ONE 19, no. 4 (2024): e0297122. http://dx.doi.org/10.1371/journal.pone.0297122.

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Site specific biotinylation of AviTagged recombinant proteins using BirA enzyme is a widely used protein labeling technology. However, due to the incomplete biotinylation reactions and the lack of a purification method specific for the biotinylated proteins, it is challenging to purify the biotinylated sample when mixed with the non-biotinylated byproduct. Here, we have developed a monoclonal antibody that specifically recognizes the non-biotinylated AviTag but not the biotinylated sequence. After a ten-minute incubation with the resin that is conjugated with the antibody, the non-biotinylated

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18

Wang, Xiaoxia, Zhou Zhang, Yun Wang, et al. "Enrichment of Total Flavonoids and Licochalcone A from Glycyrrhiza inflata Bat. Residue Based on a Combined Membrane–Macroporous Resin Process and a Quality-Control Study." Molecules 29, no. 10 (2024): 2282. http://dx.doi.org/10.3390/molecules29102282.

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Glycyrrhiza inflata Bat. produces a lot of licorice waste after water extraction, which also retains abundant total flavonoids (TFs) and licochalcone A. However, licorice residue is often wasted due to the lack of good utilization of resources in practical applications. This study first screened the optimal membrane pore size and resin type and then explored the mechanism and conditions of the adsorption of TFs on the resin. Then, different combinations and sequences of membrane and macroporous resin (MR) methods were investigated. It was found that using the membrane method for initial purifi

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19

Li, Hui, Jiayu Lin, Baoqing Bai, et al. "Study on Purification, Identification and Antioxidant of Flavonoids Extracted from Perilla leaves." Molecules 28, no. 21 (2023): 7273. http://dx.doi.org/10.3390/molecules28217273.

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The flavonoids from Perilla leaves were extracted using flash extraction assisted by ultrasonic extraction with ethanol. Subsequently, macroporous resin was employed for the isolation and purification of these flavonoids, followed by an investigation into their antioxidant activity. The process conditions for the extraction of flavonoids from Perilla leaves were designed and optimized using a one-way experiment combined with a response surface methodology. The optimal extraction conditions were determined as follows: the liquid–solid ratio was 20:1, ethanol volume fraction of 60%, ultrasound t

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20

Hou, Mengyang, Wenzhong Hu, Zhilong Xiu, et al. "Preparative Purification of Total Flavonoids from Sophora tonkinensis Gagnep. by Macroporous Resin Column Chromatography and Comparative Analysis of Flavonoid Profiles by HPLC-PAD." Molecules 24, no. 17 (2019): 3200. http://dx.doi.org/10.3390/molecules24173200.

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For the full development and utilization of Sophora tonkinensis Gagnep., this study was primarily intended to established a simple and efficient approach for the preparative purification of total flavonoids from S. tonkinensis by macroporous resin column chromatography (MRCC). The adsorption and desorption characteristics of the total flavonoids on ten macroporous resins were first studied, and AB-8 resin was chosen as the most suitable, and the adsorption data were best fitted to the pseudo-second-order kinetics model and Langmuir isotherm model. Furthermore, the technological parameters for

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21

Liu, Yan-Bo, Wang Yao, Shuai Meng, Xiang-Bao Meng, Zhong-Jun Li, and Qing-Hua Lou. "A novel cobalt(0) alkyne complex assisted “capture and release” strategy for oligosaccharide rapid assembly." Organic Chemistry Frontiers 5, no. 13 (2018): 2098–102. http://dx.doi.org/10.1039/c8qo00324f.

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22

Dong, Wanyuan, and Yifeng Li. "CH1-specific affinity resins possess the potential of separating heterodimer from homodimers in asymmetric bispecific antibody purification." Journal of Biological Methods 11, no. 3 (2024): e99010020. http://dx.doi.org/10.14440/jbm.2024.0026.

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CaptureSelect CH1-XL and Praesto 70 CH1 are two affinity media that specifically bind to the CH1 domain of an antibody. In the current work, we first demonstrated that these two CH1-specific affinity media bound to different monoclonal antibodies (mAbs) with varied strengths under identical conditions. We previously had observed the same on a Protein L-conjugated resin and showed that such a property could facilitate homodimer removal in asymmetric bispecific antibody (bsAb) purification. Next, using Praesto 70 CH1, we showed that a small difference in binding between two mAbs could be signifi

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23

Loibl, S. F., Z. Harpaz, R. Zitterbart, and O. Seitz. "Total chemical synthesis of proteins without HPLC purification." Chemical Science 7, no. 11 (2016): 6753–59. http://dx.doi.org/10.1039/c6sc01883a.

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24

Chen, Xusheng, Qin Li, Honggang He, Jianhua Zhang та Zhonggui Mao. "Effect of ion form of the ion-exchange resin on ε-poly-l-lysine purification from microbial fermentation broth". RSC Advances 9, № 21 (2019): 12174–81. http://dx.doi.org/10.1039/c9ra00493a.

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25

Li, Shangyong, Linna Wang, Xuehong Chen, Mi Sun, and Yantao Han. "Design and Synthesis of a Chitodisaccharide-Based Affinity Resin for Chitosanases Purification." Marine Drugs 17, no. 1 (2019): 68. http://dx.doi.org/10.3390/md17010068.

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Chitooligosaccharides (CHOS) have gained increasing attention because of their important biological activities. Enhancing the efficiency of CHOS production essentially requires screening of novel chitosanase with unique characteristics. Therefore, a rapid and efficient one-step affinity purification procedure plays important roles in screening native chitosanases. In this study, we report the design and synthesis of affinity resin for efficient purification of native chitosanases without any tags, using chitodisaccharides (CHDS) as an affinity ligand, to couple with Sepharose 6B via a spacer,

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26

Anvari, Masoumeh, and Gholam Khayati. "Separation and purification of rebaudioside A from extract of Stevia Rebaudiana leaves by macroporous adsorption resins." Polish Journal of Chemical Technology 18, no. 1 (2016): 127–32. http://dx.doi.org/10.1515/pjct-2016-0019.

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Abstract The separation and purification of rebaudioside A from Stevia rebaudiana crude extracts (Steviosides) by macroporous resin were optimized by Taguchi orthogonal array (OA) experimental design methodology. This approach was applied to evaluate the influence of five factors (adsorption temperature, desorption time, elution solution ratio, adsorption volume and type of resin) on the rebaudioside A yield. The percentage contribution of each factor was also determined. The results showed that elution solution ratio and adsorption volume made the greatest (59.6%) and the lowest (1.3%) contri

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27

Younes, Ali, Cyrille Alliot, Jafar Sunga Ali, et al. "Production of polonium from bismuth and purification using TBP resin and Sr resin." Journal of Radioanalytical and Nuclear Chemistry 324, no. 2 (2020): 823–28. http://dx.doi.org/10.1007/s10967-020-07109-5.

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28

Wen, Yao, Huaguo Chen, Xin Zhou, Qingfang Deng, Chao Zhao, and Xiaojian Gong. "A polyamide resin based method for adsorption of anthocyanins from blackberries." New Journal of Chemistry 40, no. 4 (2016): 3773–80. http://dx.doi.org/10.1039/c6nj00054a.

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29

Westcott, Jay Y., Kirk M. Maxey, Jim MacDonald, and Sally E. Wenzel. "Immunoaffinity Resin for Purification of Urinary Leukotriene E4." Prostaglandins & Other Lipid Mediators 55, no. 5-6 (1998): 301–21. http://dx.doi.org/10.1016/s0090-6980(98)00027-6.

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30

Xu, Jie, Jiawen Zhu, Kui Chen, Yanyang Wu, and Jiji Gu. "ENRICHMENT AND PURIFICATION OF BITESPIRAMYCIN USING MACROPOROUS RESIN." Chemical Engineering Communications 199, no. 10 (2012): 1320–33. http://dx.doi.org/10.1080/00986445.2012.682322.

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31

Porter, C. E., F. D. Riley, R. D. Vandergrift, and L. K. Felker. "Fermium Purification Using Teva™ Resin Extraction Chromatography." Separation Science and Technology 32, no. 1-4 (1997): 83–92. http://dx.doi.org/10.1080/01496399708003188.

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32

Matos, Tiago, João A. Queiroz, and Leif Bülow. "Plasmid DNA purification using a multimodal chromatography resin." Journal of Molecular Recognition 27, no. 4 (2014): 184–89. http://dx.doi.org/10.1002/jmr.2349.

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33

Corbett, Derek B., Changyoung Hong, Richard Venditti, Hasan Jameel, and Sunkyu Park. "Hydrophobic resin treatment of hydrothermal autohydrolysate for prebiotic applications." RSC Advances 9, no. 55 (2019): 31819–27. http://dx.doi.org/10.1039/c9ra06018a.

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34

Mundodi, Suparna, Porecha Rishi, Anderson Murray, and Rapicavoli Nicole. "FPLC in a pipette tip (P3297)." Journal of Immunology 190, no. 1_Supplement (2013): 211.6. http://dx.doi.org/10.4049/jimmunol.190.supp.211.6.

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Abstract Molecular characterization of the immune response and how this process is implemented requires the purification of native proteins and antibodies. There are numerous purification methods to isolate proteins of interest, but these methods usually require multiple steps to attain the level of purity required for most studies. The Rainin PureSpeed Protein Purification System utilizes pipette tips containing purification resin at their distal end interfaced with an E4 XLS pipette to simplify enrichment procedures for virtually all proteins. The E4 XLS pipette, when set up within a 96-deep

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35

Stapleton, IW. "A simple method of polyamine purification." Australian Journal of Chemistry 38, no. 4 (1985): 633. http://dx.doi.org/10.1071/ch9850633.

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A simple procedure for the large-scale purification of commercial polyethyleneamines (H2N[CH2CH2NH]nH where n = 2-5) is described in which the per- tosylate salt separates as a crystalline solid from aqueous solution. The salts require no further purification except for pentaethylenehexamine (n = 5), which requires recrystallization from water. The free bases are regenerated from the tosylate salt by an anion-exchange resin.

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36

Wang, Yong, Yu Bo Wang, Wen Ge Zhang, et al. "Identification and Separation of the Novel Antifungal Antibiotic of Streptomyces Hygroscopicus BOS-013 Strain by Simulated Moving Bed Chromatography." Advanced Materials Research 485 (February 2012): 245–48. http://dx.doi.org/10.4028/www.scientific.net/amr.485.245.

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BOS-013 actinomycete strain was obtained by separating difference soil of areas from ChangBai Mountain. The antifungal antibiotic produced by Streptomyces Hygroscopicus BOS-013 was firstly purified by means of macro-porous adsorbent resin and thin-layer chromatography. Separation and Purification of the antifungal antibiotic from its fermentation broth of streptomyces hygroscopicus BOS-013 strain were further carried out by Simulated Moving Bed Chromatography and then the crystal of the antibiotic with high purity was got. In this paper, the methods of purification by adsorbing of micro-porous

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Barozzi, Annalisa, R. Ashton Lavoie, Kevin N. Day, Raphael Prodromou, and Stefano Menegatti. "Affibody-Binding Ligands." International Journal of Molecular Sciences 21, no. 11 (2020): 3769. http://dx.doi.org/10.3390/ijms21113769.

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While antibodies remain established therapeutic and diagnostic tools, other protein scaffolds are emerging as effective and safer alternatives. Affibodies in particular are a new class of small proteins marketed as bio-analytic reagents. They feature tailorable binding affinity, low immunogenicity, high tissue permeation, and high expression titer in bacterial hosts. This work presents the development of affibody-binding peptides to be utilized as ligands for their purification from bacterial lysates. Affibody-binding candidates were identified by screening a peptide library simultaneously aga

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Arakawa, Tsutomu, Yui Tomioka, Masataka Nakagawa, et al. "Non-Affinity Purification of Antibodies." Antibodies 12, no. 1 (2023): 15. http://dx.doi.org/10.3390/antib12010015.

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Currently, purification of antibodies is mainly carried out using a platform technology composed primarily of Protein A chromatography as a capture step, regardless of the scale. However, Protein A chromatography has a number of drawbacks, which are summarized in this review. As an alternative, we propose a simple small-scale purification protocol without Protein A that uses novel agarose native gel electrophoresis and protein extraction. For large-scale antibody purification, we suggest mixed-mode chromatography that can in part mimic the properties of Protein A resin, focusing on 4-Mercapto-

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Zhao, Tao, Lin Ma, Jing Jun Du, Liang Zhang, and Xiao Yan Wu. "Purification of Hulless Barley Anthocyanins with Macroporous Resins." Advanced Materials Research 236-238 (May 2011): 2701–4. http://dx.doi.org/10.4028/www.scientific.net/amr.236-238.2701.

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The purification effects of six macroporous adsorption resins on total anthocyanins of hulless barley were compared. The results showed that XAD7HP was the best resin for purification of hulless barley anthocyanins due to its excellent adsorption and desorption capability. The optimal technological parameters were: the pH of sample solution was 3.0, the concentration of sample solution was 21.6 mg/L, the adsorption flow velocity was 1.0 mL/min, the eluting velocity is 1.0 mL/min, and the eluent was about 6.6 bed volumes of 80% ethanol. Reutilization test showed that the XAD7HP resin could be u

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40

Zhang, Huaicheng, Haolin Liu, Wei Wang, et al. "A Novel Synthesis of Highly Efficient Antimicrobial Quaternary Ammonium Pyridine Resin and Its Application in Drinking Water Treatment." Polymers 17, no. 13 (2025): 1885. https://doi.org/10.3390/polym17131885.

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Multifunctional water-treatment materials urgently need to be developed to avoid normal organic matter, inorganic anions, resistant bacteria, and hazardous disinfection by-products in conventional drinking water treatment strategies. While quaternary ammonium pyridine resins (QAPRs) possess porous adsorption structures and incorporate antibacterial groups, enabling simultaneous water disinfection and purification, their limited bactericidal efficacy hinders broader utilization. Therefore, a deeper understanding of the structure-dependent antimicrobial mechanism in QAPRs is crucial for improvin

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41

Song, Ilchan, Yang Joo Kang, Su-Lim Choi, et al. "Purification of plant-derived anti-virus mAb through optimized pH conditions for coupling between protein A and epoxy-activated beads." PeerJ 7 (May 21, 2019): e6828. http://dx.doi.org/10.7717/peerj.6828.

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The main goal of this research was to determine optimum pH conditions for coupling between protein A and epoxy-activated Sepharose beads for purification of monoclonal antibodies (mAbs) expressed in plants. To confirm the effect of pH conditions on purification efficacy, epoxy-activated agarose beads were coupled to protein A under the pH conditions of 8.5, 9.5, 10.5, and 11.5 (8.5R, 9.5R, 10.5R, and 11.5R, respectively). A total of 300 g of fresh leaf tissue of transgenic Arabidopsis expressing human anti-rabies mAb (mAbP) SO57 were harvested to isolate the total soluble protein (TSP). An equ

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42

Hay, Iain D., Jinping Du, Natalie Burr, and Bernd H. A. Rehm. "Bioengineering of Bacteria To Assemble Custom-Made Polyester Affinity Resins." Applied and Environmental Microbiology 81, no. 1 (2014): 282–91. http://dx.doi.org/10.1128/aem.02595-14.

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ABSTRACTProof of concept for thein vivobacterial production of a polyester resin displaying various customizable affinity protein binding domains is provided. This was achieved by engineering various protein binding domains into a bacterial polyester-synthesizing enzyme. Affinity binding domains based on various structural folds and derived from molecular libraries were used to demonstrate the potential of this technique. Designed ankyrin repeat proteins (DARPins), engineered OB-fold domains (OBodies), and VHHdomains from camelid antibodies (nanobodies) were employed. The respective resins wer

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Ozturk, Fatih, and Mehmet Emin Uslu. "Comparison of various columns used in anion exchange chromatography method for mAb purification." Biotech Studies 34, SI (2025): 73–82. https://doi.org/10.38042/biotechstudies.1733671.

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The impact of resins with varying ligands and pH levels on human IgG4 protein was analysed using anion exchange chromatography. Initially, a resin screening study involving five different positively charged ligands from four different brands was conducted on largely purified monoclonal antibodies, following Protein A capture. Subsequently, the influence of pH levels (6, 7, and 8.1) on an IgG4 protein with an isoelectric point of 6.9 was assessed using a single resin. Throughout the resin screening, all protein quality analyses were performed to identify the resin with the most compatible ligan

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Meile, Kristine, and Aivars Zhurinsh. "Preparative Solid Phase Extraction for the Purification of Levoglucosan Obtained from Lignocellulose." Key Engineering Materials 721 (December 2016): 82–86. http://dx.doi.org/10.4028/www.scientific.net/kem.721.82.

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Automated preparative scale solid phase extraction (SPE) has been used to separate levoglucosan – a valuable platform chemical from the liquid products of wood pyrolysis. The sorbent for SPE was a strongly basic anion exchange resin in OH-form. Separation of levoglucosan could be done with water as the eluent, the regeneration of the resin was done with a NaCl solution. Up-scaling the purification of levoglucosan is a step forward industrial production of this chemical.

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Jiao, Wei, Erhu Liu, Yao Luo, Yanxin Niu, and Yongxue Guo. "Adsorption and Desorption Characteristics of Simiao Yong’an Decoction on Macroporous Adsorption Resins." Separations 12, no. 4 (2025): 84. https://doi.org/10.3390/separations12040084.

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In this study, to identify an appropriate macroporous resin for the purification of the crude extract derived from Simiao Yong’an Decoction (SMYAD), five distinct resins were evaluated based on their adsorption and desorption performance. Through this comparative analysis, D101 macroporous adsorption resin was determined to be the most suitable candidate. Investigations into the adsorption mechanism revealed that the process followed pseudo-second-order kinetics and was well described by the Langmuir isotherm model. Thermodynamic analysis further indicated the spontaneous and exothermic nature

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Wang, Jiong Ran, Yan Zhou Hu, Xiao Han, and Ke Ding. "Separation and Purification of Zizyphusine, Spinosin, and 6′′′-Feruloylspinosin From Zizyphi Spinosi Semen." Natural Product Communications 15, no. 3 (2020): 1934578X2091055. http://dx.doi.org/10.1177/1934578x20910556.

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The methods of extraction, separation, and purification of zizyphusine, spinosin, and 6′′′-feruloylspinosin from Zizyphi Spinosi Semen ( ZSS) were investigated. From 31.2 g defatted ZSS, 47.7 mg zizyphusine, 57.8 mg spinosin, and 80.5 mg 6′′′-feruloylspinosin were obtained after ultrasonic extraction, purification with macroporous resin, separation by flash chromatography, and purification by high-pressure preparative chromatography. The purities of zizyphusine, spinosin, and 6′′′-feruloylspinosin were 98.6%, 98.2%, and 99.0%, respectively, and their yields 85.0%, 82.8%, and 83.0%. The methods

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Zhao, Chan, Jian Xu, Yao Liu, et al. "Extraction and Purification of Flavonoids and Antiviral and Antioxidant Activities of Polygonum perfoliatum L." Molecules 30, no. 1 (2024): 29. https://doi.org/10.3390/molecules30010029.

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The aim of the present study was to optimize the process parameters for the extraction and purification of total flavonoids from Polygonum perfoliatum L., in addition to analyzing their chemical composition and evaluating their activity against varicella-zoster virus (VZV) and antioxidant activity. The optimum extraction process was determined using one-way and response surface methods with the following conditions: ethanol concentration of 82.00%, temperature of 90.29 °C, solid-to-liquid ratio of 1:32.78 g/mL, extraction time of 1.5 h, and two extractions with a yield of 14.98 ± 0.11 mg/g. Pu

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Zhang, Wei-Wei, та Perry Churchill. "Purification of D-β-hydroxybutyrate dehydrogenase from rat brain". Biochemistry and Cell Biology 68, № 6 (1990): 980–83. http://dx.doi.org/10.1139/o90-144.

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D-β-Hydroxybutyrate dehydrogenase (BDH), a lipid-requiring enzyme, has been purified to homogeneity from rat brain using a new improved method. The purified rat brain BDH has a subunit molecular mass of 31 kilodaltons on sodium dodecyl sulphate – polyacrylamide gel electrophoresis. The apoenzyme, i.e., the enzyme devoid of phospholipid, has no activity, but can be activated by phospholipid to a specific activity of 125 μmol/(min∙mg). This is 625-fold greater than the activity in the mitochondrial fraction. The new purification procedure involves chromatography using a quaternary amine Sepharos

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Wang, Yuqiong, Xiaoxiao Huang, Yali Sun, Shouqian Zhao, and Yahui Yue. "A new method for the separation of LREEs in geological materials using a single TODGA resin column and its application to the determination of Nd isotope compositions by MC-ICPMS." Analytical Methods 9, no. 23 (2017): 3531–40. http://dx.doi.org/10.1039/c7ay00966f.

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Roncal, Tomás, Ainhoa Aguirre, Yolanda Belaustegui, and Elisabet Andrés. "Recovery and purification of acetic acid from extremely diluted solutions using a mixed bed ion exchange resin – technical feasibility." RSC Advances 15, no. 1 (2025): 477–88. https://doi.org/10.1039/d4ra08341e.

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A downstream process for the recovery, purification and concentration of acetic acid from an extremely diluted solution, involving demineralization, treatment with a mixed bed ion exchange resin and step-elution with H2SO4.

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