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Journal articles on the topic 'Resistance typing'

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Published: 4 June 2021

Last updated: 14 February 2022

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1

HERRERA, S., R. CABRERA, M. M. RAMIREZ, M. A. USERA, and M. A. ECHEITA. "Use of AFLP, plasmid typing and phenotyping in a comparative study to assess genetic diversity of Shigella flexneri strains." Epidemiology and Infection 129, no. 3 (2002): 445–50. http://dx.doi.org/10.1017/s0950268802007562.

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Shigella flexneri infections are one of the main causes of acute diarrhoea in Cuba. Twenty strains isolated from sporadic cases in nine different Cuban provinces were characterized. Serotyping, antibiotic-resistance typing, plasmid-typing and AFLP-typing were used to determine their suitability for use in epidemiological studies of S. flexneri. The predominant serotypes were serotype 6 (35%) and serotype 2 (35%). Eleven different plasmid profiles were detected (Diversity Index=0·92). AFLP-typing discriminated 12 different patterns (DI=0·95), these patterns were not coincident with plasmid-typi

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Sadeghifard, Nourkhoda, Reza Ranjbar, Javad Zaeimi, et al. "Antimicrobial susceptibility, plasmid profiles, and RAPD-PCR typing of Acinetobacter bacteria." Asian Biomedicine 4, no. 6 (2010): 901–11. http://dx.doi.org/10.2478/abm-2010-0118.

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Abstract Background: Multiple-drug resistant Acinetobacter have widely spread in the last decades imposing a serious nosocomial source of infection. Nevertheless, little knowledge was gaimed on tracing the development of antibiotic resistance in Acinetobacter species. Objectives: Explore Acinetobacter spp. via antimicrobial susceptibility, plasmid profiles, and random amplified polymorphism DNA polymerase chain reaction (RAPD-PCR) typing. Methods: One hundred twelve Acinetobacter isolates (including 66 A. baumannii and 46 non-Acinetobacter baumannii strains) were obtained from three university

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Adaska, J. M., A. J. Silva, A. C. B. Berge, and W. M. Sischo. "Genetic and Phenotypic Variability among Salmonella enterica Serovar Typhimurium Isolates from California Dairy Cattle and Humans." Applied and Environmental Microbiology 72, no. 10 (2006): 6632–37. http://dx.doi.org/10.1128/aem.01038-06.

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ABSTRACT Fifty-six human and 24 adult dairy cattle isolates of Salmonella enterica serovar Typhimurium from a single county in California were compared using ribotyping, insertion sequence typing (IS200), pulsed-field gel electrophoresis, plasmid typing, phage typing, and antimicrobial resistance testing. The majority of the isolates fell into one of two groups which were phage types DT104 and DT193. Combining the information from all typing methods, a total of 45 different “clusters” were defined, with 35 of those including only a single isolate. The library of isolates had a high degree of v

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Ogbolu, Do, та Ma Webber. "Carbapenem Resistance in Gram-negative Bacteria in South-western Nigeria: The Role of Extended-spectrum β-lactamase CTX-M-15". West Indian Medical Journal, Vol 67, Issue 4: 2018 (31 грудня 2018): 344–49. http://dx.doi.org/10.7727/wimj.2017.122.

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Objective: To determine the role of extended-spectrum β-lactamases in carbapenem-resistant Gram-negative bacteria from south-western Nigeria. Methods: Twenty-seven carbapenem-resistant isolates that were found to be non-carbapenemase producers (15 Escherichia coli, 9 Klebsiella pneumoniae and 3 Pseudomonas aeruginosa) were further studied. These isolates were subjected to analysis including phenotypic and genotypic detection of various β-lactamases, efflux activity, outer membrane protein, plasmids replicon typing, detection of transferable genes and resistances and typing using random amplifi

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Jawad, Saba J. "Multi Locus Sequence Typing of Multi Drug Resistance Klebsiella Pneumoniae Isolated from different Clinical Samples." International Journal of Psychosocial Rehabilitation 24, no. 5 (2020): 3107–15. http://dx.doi.org/10.37200/ijpr/v24i5/pr202018.

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Cereda, Rosangela F., Ana C. Gales, Suzane Silbert, Ronald N. Jones, and Helio S. Sader. "Molecular Typing and Antimicrobial Susceptibility of Vancomycin-ResistantEnterococcus faeciumin Brazil." Infection Control & Hospital Epidemiology 23, no. 1 (2002): 19–22. http://dx.doi.org/10.1086/501962.

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AbstractObjectives:To characterize vancomycin-resistant enterococci (VRE) isolates and to evaluate the mode of dissemination of this pathogen in Brazil.Design:We collected 22 vancomycin-resistantEnterococcus faeciumisolates from 6 medical centers in Sao Paulo, Brazil, and 1 isolate from a medical center in Curitiba, Brazil.Participants:All Brazilian hospitals that had identified vancomycin-resistantE. faeciumup to the beginning of this study (late 1999) contributed isolates to the study.Methods:The isolates were susceptibility tested using the broth microdilution method and the E-test. The pre

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Nemes-Nikodém, Éva, Alexandra Brunner, Béla Tóth, Veronika Tóth, András Bánvölgyi, and Eszter Ostorházi. "Antimicrobal resistance ofNeisseria gonorrhoeaestrains in Hungary." Orvosi Hetilap 156, no. 6 (2015): 226–29. http://dx.doi.org/10.1556/oh.2015.30079.

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Introduction: European guidelines on the treatment of Neisseria gonorrhoeae are based mostly on Western European data, although these recommendations may not be optimised for the circumstances in Hungary. Aim: The aim of the authors was to assess current antimicrobial resistance of Neisseria gonorrhoeae strains in order to enhance gonococcal antimicrobial surveillance in Hungary. Neisseria gonorrhoeae strains were isolated at the National Center of Sexually Transmitted Infections at the Department of Dermatology, Venerology and Dermatooncology of Semmelweis University in the period between Jan

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8

O'Brien, Frances, and Phil Giffard. "Two approaches to Staphylococcus aureus typing." Microbiology Australia 29, no. 3 (2008): 131. http://dx.doi.org/10.1071/ma08131.

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Typing of Staphylococcus aureus is carried out to detect outbreaks, monitor transmission and infer virulence and resistance properties. There are now many different extant typing methods. This article summarises the broad classes of typing approaches and describes two methods. One is based on micro-array technology while the other is based upon the interrogation of polymorphic sites using real-time PCR.

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Wong, S., K. Pabbaraju, K. Ho, V. Khurana, and J. D. Fox. "Influenza A detection, typing and assessment of antiviral resistance." Journal of Clinical Virology 36 (January 2006): S18. http://dx.doi.org/10.1016/s1386-6532(06)80762-6.

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10

Carattoli, Alessandra. "Plasmids in Gram negatives: Molecular typing of resistance plasmids." International Journal of Medical Microbiology 301, no. 8 (2011): 654–58. http://dx.doi.org/10.1016/j.ijmm.2011.09.003.

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11

Demczuk, W., S. Sidhu, M. Unemo, et al. "Neisseria gonorrhoeae Sequence Typing for Antimicrobial Resistance, a Novel Antimicrobial Resistance Multilocus Typing Scheme for Tracking Global Dissemination of N. gonorrhoeae Strains." Journal of Clinical Microbiology 55, no. 5 (2017): 1454–68. http://dx.doi.org/10.1128/jcm.00100-17.

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ABSTRACTA curated Web-based user-friendly sequence typing tool based on antimicrobial resistance determinants inNeisseria gonorrhoeaewas developed and is publicly accessible (https://ngstar.canada.ca). TheN. gonorrhoeaeSequence Typing for Antimicrobial Resistance (NG-STAR) molecular typing scheme uses the DNA sequences of 7 genes (penA,mtrR,porB,ponA,gyrA,parC, and 23S rRNA) associated with resistance to β-lactam antimicrobials, macrolides, or fluoroquinolones. NG-STAR uses the entirepenAsequence, combining the historical nomenclature forpenAtypes I to XXXVIII with novel nucleotide sequence de

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Xu, Feili, Junping Wang, Yunchang Guo, et al. "Antibiotic resistance, biochemical typing, and PFGE typing of Bifidobacterium strains commonly used in probiotic health foods." Food Science and Biotechnology 27, no. 2 (2018): 467–77. http://dx.doi.org/10.1007/s10068-018-0320-6.

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Silva, Vanessa, Sara Hermenegildo, Catarina Ferreira, et al. "Genetic Characterization of Methicillin-Resistant Staphylococcus aureus Isolates from Human Bloodstream Infections: Detection of MLSB Resistance." Antibiotics 9, no. 7 (2020): 375. http://dx.doi.org/10.3390/antibiotics9070375.

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In this study we aimed to characterize antimicrobial resistance in methicillin-resistant Staphylococcus aureus (MRSA) isolated from bloodstream infections as well as the associated genetic lineages of the isolates. Sixteen MRSA isolates were recovered from bacteremia samples from inpatients between 2016 and 2019. The antimicrobial susceptibility of these isolates was tested by the Kirby–Bauer disk diffusion method against 14 antimicrobial agents. To determine the macrolide–lincosamide–streptogramin B (MLSB) resistance phenotype of the isolates, erythromycin-resistant isolates were assessed by

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Witte, Wolfgang, Christine Braulke, Christa Cuny, Dagmar Heuck, and Michael Kresken. "Changing Pattern of Antibiotic Resistance in Methicillin-ResistantStaphylococcus aureusFrom German Hospitals." Infection Control & Hospital Epidemiology 22, no. 11 (2001): 683–86. http://dx.doi.org/10.1086/501845.

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AbstractObjective:To investigate the background of changes of resistance phenotypes in methicillin-resistantStaphylococcus aureus(MRSA) from nosocomial infections in German hospitals by molecular typing and identification of particular resistance genes.Methods:Isolates from the network for monitoring the spread of MRSA in Germany were subjected to quantitative susceptibility testing, to molecular typing, and to polymerase chain reaction identification of resistance genes.Participants:The network consists of 175 German clinical microbiological laboratories collaborating with the German Referenc

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Rafei, Rayane, Marwan Osman, Fouad Dabboussi, and Monzer Hamze. "Update on the epidemiological typing methods for Acinetobacter baumannii." Future Microbiology 14, no. 12 (2019): 1065–80. http://dx.doi.org/10.2217/fmb-2019-0134.

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The outstanding ability of Acinetobacter baumannii to cause outbreaks and acquire multidrug resistance motivated the development of a plethora of typing techniques, which can help infection preventionists and hospital epidemiologists to more efficiently implement intervention controls. Nowadays, the world is witnessing a gradual transition from traditional typing methodology to whole genome sequencing-based approaches. Such approaches are opening new prospects and applications never achieved by existing typing methods. Herein, we provide the reader with an updated review on A. baumannii typing

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Fernández-Naval, Candela, Maider Arando, Mateu Espasa, et al. "Multilocus sequence typing of Treponema pallidum subsp. pallidum in Barcelona." Future Microbiology 16, no. 13 (2021): 967–76. http://dx.doi.org/10.2217/fmb-2021-0037.

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Aim: To implement the multilocus sequence typing (MLST) methodology in syphilis samples previously characterized by enhanced CDC typing (ECDCT) and macrolide resistance. Materials & methods: MLST was performed on genital ulcer and blood samples by analyzing a region of the tp0136, tp0548 and tp0705 loci using Sanger sequencing. Results: Up to 59/85 (69.4%) of genital ulcer and 4/39 (10.3%) of whole blood samples were fully typed. The most frequent profiles were 1.3.1 (56%) and 1.1.1 (11%). All the 1.3.1 samples typed carried the A2058G mutation, responsible for macrolide resistance. MLST a

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Akagi, Kenichi. "A Computer Keyboard Key Feel Study in Performance and Preference." Proceedings of the Human Factors Society Annual Meeting 36, no. 5 (1992): 523–27. http://dx.doi.org/10.1177/154193129203600511.

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A study was conducted to compare user preference and performance of four keyboards having different key force and travel characteristics. Two keyboards had linear spring key action, one with low (key force) resistance (42.5 grams) and one with high resistance (70.9 grams). The other two keyboards had tactile (snap) action, one with low resistance (35.5 grams) and one with high resistance (70.9 grams). All four keyboards were manufactured by the same company, and were visually identical in size, layout, color, etc. There was no difference in typing sound and traveling distance among the four ke

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El-Astal, Zacharia, Najjar Khamis, Nehama Peled, Ron Dagan, and Pablo Yagupsky. "ANTIMICROBIAL RESISTANCE AND TYPING OF PNEUMOCOCCI IN GAZA STRIP CHILDREN." Pediatric Infectious Disease Journal 16, no. 9 (1997): 905–7. http://dx.doi.org/10.1097/00006454-199709000-00016.

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Carattoli, Alessandra, Vivi Miriagou, Alessia Bertini та ін. "Replicon Typing of Plasmids Encoding Resistance to Newer β-Lactams". Emerging Infectious Diseases 12, № 7 (2006): 1145–48. http://dx.doi.org/10.3201/eid1207.051555.

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Raaf, Naïma, Wahiba Amhis, Houria Saoula, et al. "Prevalence, antibiotic resistance, and MLST typing ofHelicobacter pyloriin Algiers, Algeria." Helicobacter 22, no. 6 (2017): e12446. http://dx.doi.org/10.1111/hel.12446.

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Gillespie, M. T., B. R. Lyon, and R. A. Skurray. "Typing of methicillin-resistant Staphylococcus aureus by antibiotic resistance phenotypes." Journal of Medical Microbiology 31, no. 1 (1990): 57–64. http://dx.doi.org/10.1099/00222615-31-1-57.

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Bitar, Ibrahim, Roy A. Khalaf, Houda Harastani, and Sima Tokajian. "Identification, Typing, Antifungal Resistance Profile, and Biofilm Formation ofCandida albicansIsolates from Lebanese Hospital Patients." BioMed Research International 2014 (2014): 1–10. http://dx.doi.org/10.1155/2014/931372.

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As leading opportunistic fungal pathogens identification and subtyping ofCandidaspecies are crucial in recognizing outbreaks of infection, recognizing particularly virulent strains, and detecting the emergence of drug resistant strains. In this study our objective was to compare identification ofCandida albicansby the hospitals through the use of conventional versus identification based on the ITS (Internal Transcribed Spacer) and to assess biofilm forming capabilities, drug resistance patterns and correlate these with MLST typing. ITS typing revealed a 21.2% hospital misidentification rate. M

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Vignaroli, C., G. M. Luna, C. Rinaldi, A. Di Cesare, R. Danovaro, and F. Biavasco. "New Sequence Types and Multidrug Resistance among Pathogenic Escherichia coli Isolates from Coastal Marine Sediments." Applied and Environmental Microbiology 78, no. 11 (2012): 3916–22. http://dx.doi.org/10.1128/aem.07820-11.

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ABSTRACTThe spread of antibiotic-resistant microorganisms is widely recognized, but data about their sources, presence, and significance in marine environments are still limited. We examined 109Escherichia colistrains from coastal marine sediments carrying virulence genes for antibiotic susceptibility, specific resistance genes, prevalence of class 1 and 2 integrons, and sequence type. Antibiotic resistance was found in 35% of strains, and multiple resistances were found in 14%; the resistances detected most frequently were against tetracycline (28%), ampicillin (16.5%), trimethoprim-sulfameth

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Santos, Renata P., Fernando N. Souza, Ana Claudia D. Oliveira, et al. "Molecular Typing and Antimicrobial Susceptibility Profile of Staphylococcus aureus Isolates Recovered from Bovine Mastitis and Nasal Samples." Animals 10, no. 11 (2020): 2143. http://dx.doi.org/10.3390/ani10112143.

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In the present study, we aimed to determine the antimicrobial resistance and molecular typing of Staphylococcus aureus recovered from transient and persistent intramammary infections and nares/muzzles in dairy cows. We investigated the antimicrobial resistance of 189 S. aureus strains using a broad antimicrobial susceptibility profile. Furthermore, 107 S. aureus isolates were strain-typed using staphylococcal protein-A (spa) typing. A large proportion of strains exhibited multidrug resistance to antimicrobials, including resistance to critically important antimicrobials, although no methicilli

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Selyanskaya, N. A., I. V. Arkhangelskaya, A. S. Vodopianov, et al. "TYPING OF VIBRIO CHOLERAE NON O1/NON O139 STRAINS, ISOLATED IN ROSTOV REGION IN 2014." Journal of microbiology epidemiology immunobiology, no. 1 (February 28, 2016): 3–9. http://dx.doi.org/10.36233/0372-9311-2016-1-3-9.

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Aim. Comparative study of antibiotics resistance and VNTR-typing of Vibrio cholerae non O1/ non O139 strains, isolated on the territory of Rostov region in 2014. Materials and methods. Antibioticogramms of strains were determined by serial dilution method in dense nutrient medium according to MG 4.2.2495-09 (2009). Pheno-, sero- and VNTR-typing was carried out by conventional methods. Results. The studied strains belonged to V. cholerae species, did not agglutinate with O1 and O139 sera, were atoxigenic, hemolysis-positive, did not contain genes of cholera toxin and toxin-coregulating pili ofa

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SOYER, Y., J. RICHARDS, K. HOELZER, et al. "Antimicrobial Drug Resistance Patterns among Cattle- and Human-Associated Salmonella Strains." Journal of Food Protection 76, no. 10 (2013): 1676–88. http://dx.doi.org/10.4315/0362-028x.jfp-13-018.

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During the year 2004, 178 human and 158 bovine clinical Salmonella isolates were collected across New York State to better understand the transmission dynamics and genetic determinants of antimicrobial resistance among human and bovine hosts. Serotyping, sequence typing, and pulsed-field gel electrophoresis typing results have been reported previously. Here we tested all isolates for phenotypic susceptibility to 15 antimicrobial drugs that are part of the National Antimicrobial Monitoring System bovine susceptibility panel. PCR was performed on a representative subset of unique isolates (n = 5

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Juraschek, Katharina, Maria Borowiak, Simon H. Tausch, et al. "Outcome of Different Sequencing and Assembly Approaches on the Detection of Plasmids and Localization of Antimicrobial Resistance Genes in Commensal Escherichia coli." Microorganisms 9, no. 3 (2021): 598. http://dx.doi.org/10.3390/microorganisms9030598.

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Antimicrobial resistance (AMR) is a major threat to public health worldwide. Currently, AMR typing changes from phenotypic testing to whole-genome sequence (WGS)-based detection of resistance determinants for a better understanding of the isolate diversity and elements involved in gene transmission (e.g., plasmids, bacteriophages, transposons). However, the use of WGS data in monitoring purposes requires suitable techniques, standardized parameters and approved guidelines for reliable AMR gene detection and prediction of their association with mobile genetic elements (plasmids). In this study,

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Giles, Julie A., Jason Falconio, Jeffrey D. Yuenger, Jonathan M. Zenilman, Michael Dan, and Margaret C. Bash. "Quinolone Resistance–Determining Region Mutations andporType ofNeisseria gonorrhoeaeIsolates: Resistance Surveillance and Typing by Molecular Methodologies." Journal of Infectious Diseases 189, no. 11 (2004): 2085–93. http://dx.doi.org/10.1086/386312.

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Barbadoro, Pamela, Daniela Bencardino, Elisa Carloni, et al. "Carriage of Carbapenem-Resistant Enterobacterales in Adult Patients Admitted to a University Hospital in Italy." Antibiotics 10, no. 1 (2021): 61. http://dx.doi.org/10.3390/antibiotics10010061.

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The emerging spread of carbapenemase-producing Enterobacterales (CPE) strains, in particular, Klebsiella pneumoniae and Escherichia coli, has become a significant threat to hospitalized patients. Carbapenemase genes are frequently located on plasmids than can be exchanged among clonal strains, increasing the antibiotic resistance rate. The aim of this study was to determine the prevalence of CPE in patients upon their admission and to analyze selected associated factors. An investigation of the antibiotic resistance and genetic features of circulating CPE was carried out. Phenotypic tests and

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Barbadoro, Pamela, Daniela Bencardino, Elisa Carloni, et al. "Carriage of Carbapenem-Resistant Enterobacterales in Adult Patients Admitted to a University Hospital in Italy." Antibiotics 10, no. 1 (2021): 61. http://dx.doi.org/10.3390/antibiotics10010061.

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The emerging spread of carbapenemase-producing Enterobacterales (CPE) strains, in particular, Klebsiella pneumoniae and Escherichia coli, has become a significant threat to hospitalized patients. Carbapenemase genes are frequently located on plasmids than can be exchanged among clonal strains, increasing the antibiotic resistance rate. The aim of this study was to determine the prevalence of CPE in patients upon their admission and to analyze selected associated factors. An investigation of the antibiotic resistance and genetic features of circulating CPE was carried out. Phenotypic tests and

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Jian, Ming-Jr, Yun-Hsiang Cheng, Cherng-Lih Perng, and Hung-Sheng Shang. "Molecular typing and profiling of topoisomerase mutations causing resistance to ciprofloxacin and levofloxacin inElizabethkingiaspecies." PeerJ 6 (September 12, 2018): e5608. http://dx.doi.org/10.7717/peerj.5608.

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ObjectivesSeveralElizabethkingiaspecies often exhibit extensive antibiotic resistance, causing infections associated with severe morbidity and high mortality rates worldwide. In this study, we determined fluoroquinolone susceptibility profiles of clinicalElizabethkingiaspp. isolates and investigated the resistance mechanisms.MethodsIn 2017–2018, 131Elizabethkingiaspp. isolates were recovered from specimens collected at tertiary care centers in northern Taiwan. Initial species identification using the Vitek MS system and subsequent verification by 16S rRNA sequencing confirmed the presence ofEl

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Bahnan, Wael, Fuad Hashwa, George Araj, and Sima Tokajian. "emm typing, antibiotic resistance and PFGE analysis of Streptococcus pyogenes in Lebanon." Journal of Medical Microbiology 60, no. 1 (2011): 98–101. http://dx.doi.org/10.1099/jmm.0.023317-0.

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One hundred and three Streptococcus pyogenes isolates recovered mainly from streptococcal throat infections in Lebanon were characterized by emm and PFGE typing. Thirty-three emm types and subtypes were detected among the isolates. PFGE was more discriminatory as a typing method. The prevalent emm types were emm1 (12.6 %), emm22 (8.7 %), emm28 (7.7 %), emm88 (7.7 %) and emm4 (6.8 %) and all isolates were susceptible to vancomycin and penicillin G. Ten per cent of the isolates were resistant to erythromycin and 3 % were resistant to erythromycin and clindamycin, showing the macrolide–lincosamid

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Katakweba, Abdul Sekemani, Amandus Pachificus Muhairwa, Carmen Espinosa-Gongora, Luca Guardabassi, Madundo M. A. Mtambo, and John Elmerdahl Olsen. "spa typing and antimicrobial resistance of Staphylococcus aureus from healthy humans, pigs and dogs in Tanzania." Journal of Infection in Developing Countries 10, no. 02 (2016): 143–48. http://dx.doi.org/10.3855/jidc.6790.

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Introduction: Staphylococcus aureus is an opportunistic pathogen causing infections in humans and animals. Here we report for the first time the prevalence of nasal carriage, spa typing and antimicrobial resistance of S. aureus in a Tanzanian livestock community. Methodology: Nasal swabs were taken from 100 humans, 100 pigs and 100 dogs in Morogoro Municipal. Each swab was enriched in Mueller Hinton broth with 6.5% NaCl and subcultured on chromogenic agar for S. aureus detection. Presumptive S. aureus colonies were confirmed to the species level by nuc PCR and analysed by spa typing. Antimicro

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Witte, W., and Chr Braulke. "Multiply- and methicillin-resistantStaphylococcus aureusstrains isolated in the German Democratic Republic in 1985 and 1986." Epidemiology and Infection 99, no. 3 (1987): 603–12. http://dx.doi.org/10.1017/s0950268800066450.

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SUMMARYMultiply- and methicillin-resistantStaphylococcus aureus(MRSA) strains have been isolated from five small outbreaks of nosocomial infection in five different hospitals. The MRSA were typed by phage patterns, biochemical traits, resistance phenotypes and plasmid patterns. Three different groups of strains can be distinguished. The MRSA from three outbreaks in one country share identical characters.Phage typing by the use of the International Basic Set for Phage Typing staphylococci as well as experimental phages does not completely discriminate between the strains. Attribution of several

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Koeleman, Johannes G. M., Madelon W. van der Bijl, Jeroen Stoof, Christina M. J. E. Vandenbroucke-Grauls, and Paul H. M. Savelkoul. "Antibiotic Resistance Is a Major Risk Factor for Epidemic Behavior ofAcinetobacter baumannii." Infection Control & Hospital Epidemiology 22, no. 5 (2001): 284–88. http://dx.doi.org/10.1086/501901.

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AbstractObjective:To study the presence of bacterial factors in clinical isolates ofAcinetobacterspecies in order to identify markers of epidemic potential.Design:Case-control study.Methods:Forty-six isolates ofAcinetobacterspecies, including 23 epidemic and 23 sporadic strains from different outbreaks in nine European countries, were compared for the presence of the following factors: hemagglutination, presence of capsules and fimbriae, binding to salivary mucins, resistance to drying, and antibiogram typing. Genotyping of all strains was performed by amplified fragment-length polymorphism (A

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Freitas, Ana Lúcia Peixoto de, and Afonso Luis Barth. "Antibiotic resistance and molecular typing of Pseudomonas aeruginosa: focus on imipenem." Brazilian Journal of Infectious Diseases 6, no. 1 (2002): 01–06. http://dx.doi.org/10.1590/s1413-86702002000100001.

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Spuesens, Emiel B. M., Theo Hoogenboezem, Marcel Sluijter, Nico G. Hartwig, Annemarie M. C. van Rossum, and Cornelis Vink. "Macrolide resistance determination and molecular typing of Mycoplasma pneumoniae by pyrosequencing." Journal of Microbiological Methods 82, no. 3 (2010): 214–22. http://dx.doi.org/10.1016/j.mimet.2010.06.004.

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Bentley, C. A., J. A. Frost, and B. Rowe. "Phage typing and drug resistance ofShigella sonneiisolated in England and Wales." Epidemiology and Infection 116, no. 3 (1996): 295–302. http://dx.doi.org/10.1017/s0950268800052602.

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SUMMARYPhage typing ofShigella sonneihas been used to examine isolates from the 1991–2 sonnei dysentery outbreak in England and Wales and compare them with strains isolated during and following a widespread foodborne outbreak in 1994 which was associated with consumption of imported lettuce. The distribution of phage types was different in the three periods studied with PT 3 predominating during 1991–2, PT 2 during the ‘lettuce’ outbreak in the summer months and PT 6 during the subsequent months. PT 6 was frequently associated with travel outside the UK. Variation was also seen in the distribu

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Břinda, Karel, Alanna Callendrello, Kevin C. Ma, et al. "Rapid inference of antibiotic resistance and susceptibility by genomic neighbour typing." Nature Microbiology 5, no. 3 (2020): 455–64. http://dx.doi.org/10.1038/s41564-019-0656-6.

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Mulligan, M. E., R. Y. Kwok, D. M. Citron, J. F. John, and P. B. Smith. "Immunoblots, antimicrobial resistance, and bacteriophage typing of oxacillin-resistant Staphylococcus aureus." Journal of Clinical Microbiology 26, no. 11 (1988): 2395–401. http://dx.doi.org/10.1128/jcm.26.11.2395-2401.1988.

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Villa, Laura, Aurora García-Fernández, Daniela Fortini, and Alessandra Carattoli. "Replicon sequence typing of IncF plasmids carrying virulence and resistance determinants." Journal of Antimicrobial Chemotherapy 65, no. 12 (2010): 2518–29. http://dx.doi.org/10.1093/jac/dkq347.

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Palmer, H. M., H. Young, C. Graham, and J. Dave. "Prediction of antibiotic resistance using Neisseria gonorrhoeae multi-antigen sequence typing." Sexually Transmitted Infections 84, no. 4 (2008): 280–84. http://dx.doi.org/10.1136/sti.2008.029694.

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Udo, Edet E., and Eiman Sarkhoo. "Genetic analysis of high-level mupirocin resistance in the ST80 clone of community-associated meticillin-resistant Staphylococcus aureus." Journal of Medical Microbiology 59, no. 2 (2010): 193–99. http://dx.doi.org/10.1099/jmm.0.013268-0.

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Four community-associated meticillin-resistant Staphylococcus aureus (CA-MRSA) isolates expressing high-level mupirocin resistance (MIC >1024 mg l−1) were isolated from four sites of a diabetic patient and characterized for the genetic location of their resistance determinants and typed using PFGE, staphylococcal cassette chromosome mec (SCCmec), the coagulase gene and multilocus sequence typing to ascertain their relatedness. The presence of genes for resistance to high-level mupirocin (mupA), tetracycline (tetK) and fusidic acid (far1), Panton–Valentine leukocidin (PVL), accessory gene re

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Khan, Abul Hossain, AKM Shamsuzzaman, Shyamal Kumar Paul, Md Murshed Alam, and Md Chand Mahmud. "Antimicrobial Susceptibility and Coagulase Typing of MRSA Strains at Mymensingh Medical College." Bangladesh Journal of Medical Microbiology 1, no. 2 (2016): 56–60. http://dx.doi.org/10.3329/bjmm.v1i2.21510.

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Antimicrobial susceptibility and coagulase typing of Staphylococcus aureus isolates, with particular emphasis to Methicillin Resistant S. aureus (MRSA), were studied among strains isolated from various types of specimens collected at Mymensingh. A newly developed panel of anti-sera against different coagulase enzymes was used for coagulase typing. The study included 79 strains of S. aureus and of those, 40 were identified as MRSA on the basis of resistance to oxacillin (1 gm) discs. The rate of resistance of S. aureus to different antibiotics observed: penicillin (88.61%), oxacillin (48.10%),

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Chen, Kuo-Wei, Hsiu-Jung Lo, Yu-Hui Lin, and Shu-Ying Li. "Comparison of four molecular typing methods to assess genetic relatedness of Candida albicans clinical isolates in Taiwan." Journal of Medical Microbiology 54, no. 3 (2005): 249–58. http://dx.doi.org/10.1099/jmm.0.45829-0.

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This report describes the investigation of the genetic profiles of 53 Candida albicans isolates collected from 18 hospitals in Taiwan using three PFGE-based typing methods (PFGE karyotyping, and PFGE of SfiI and BssHII restriction fragments) and one repetitive-sequence-PCR (rep-PCR) method. All four methods were able to identify clonal related isolates from the same patients. PFGE-BssHII exhibited the highest discriminatory power by discriminating 40 genotypes, followed by PFGE-SfiI (35 genotypes) and then by rep-PCR (31 genotypes), while PFGE karyotyping exhibited the lowest discriminatory po

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Denis, Olivier, Ariane Deplano, Claire Nonhoff, et al. "National Surveillance of Methicillin-Resistant Staphylococcus aureus in Belgian Hospitals Indicates Rapid Diversification of Epidemic Clones." Antimicrobial Agents and Chemotherapy 48, no. 9 (2004): 3625–29. http://dx.doi.org/10.1128/aac.48.9.3625-3629.2004.

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ABSTRACT Methicillin-resistant Staphylococcus aureus (MRSA) strains (n = 455) collected in 2001 from 100 Belgian hospitals were characterized by molecular typing and by resistance gene distribution to macrolides-lincosamides-streptogramins and to aminoglycoside antibiotics. Rapid diversification of MRSA clones, compared with results of previous surveys, was evidenced by the broad geographic distribution of seven major clones belonging to the pandemic MRSA clonal complexes 5, 8, 22, 30, and 45 by multilocus sequence typing.

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Colque Navarro, Patricia, Heriberto Fernandez, Roland Möllby, et al. "Antibiotic resistance in environmental Escherichia coli – a simple screening method for simultaneous typing and resistance determination." Journal of Water and Health 12, no. 4 (2014): 692–701. http://dx.doi.org/10.2166/wh.2014.216.

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We describe a simple and standardised screening system (AREB) for surveillance of antibiotic resistant bacteria in the environment. The system consists of 96 well microplates containing eight sets of breakpoint amounts of 10 different antibiotics. The incubated microplates are read by a desktop scanner and the plate images are analysed by special software that automatically presents the resistance data. The AREB method is combined with a rapid typing method, the PhenePlate system, which yields information on the diversity of the bacteria in the studied samples, and on the possible prevalence o

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Carattoli, Alessandra, Ea Zankari, Aurora García-Fernández, et al. "In SilicoDetection and Typing of Plasmids using PlasmidFinder and Plasmid Multilocus Sequence Typing." Antimicrobial Agents and Chemotherapy 58, no. 7 (2014): 3895–903. http://dx.doi.org/10.1128/aac.02412-14.

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ABSTRACTIn the work presented here, we designed and developed two easy-to-use Web tools forin silicodetection and characterization of whole-genome sequence (WGS) and whole-plasmid sequence data from members of the familyEnterobacteriaceae. These tools will facilitate bacterial typing based on draft genomes of multidrug-resistantEnterobacteriaceaespecies by the rapid detection of known plasmid types. Replicon sequences from 559 fully sequenced plasmids associated with the familyEnterobacteriaceaein the NCBI nucleotide database were collected to build a consensus database for integration into a

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Wittwer, M., J. Keller, T. M. Wassenaar, et al. "Genetic Diversity and Antibiotic Resistance Patterns in a Campylobacter Population Isolated from Poultry Farms in Switzerland." Applied and Environmental Microbiology 71, no. 6 (2005): 2840–47. http://dx.doi.org/10.1128/aem.71.6.2840-2847.2005.

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ABSTRACT The diversity and genetic interrelation of Campylobacter jejuni and C. coli isolated from Swiss poultry were assessed by three independent typing methods. Samples were derived prior to slaughter from 100 randomly selected flocks (five birds per flock) raised on three different farm types. The observed flock prevalence was 54% in total, with 50% for conventional and 69% for free-range farms. Birds held on farms with a confined roaming area had the lowest prevalence of 37%. Campylobacter isolates were characterized by amplified fragment length polymorphism (AFLP), restriction fragment l

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SOUSA, MARGARIDA, VANESSA SILVA, ADRIANA SILVA, et al. "Staphylococci among Wild European Rabbits from the Azores: A Potential Zoonotic Issue?" Journal of Food Protection 83, no. 7 (2020): 1110–14. http://dx.doi.org/10.4315/0362-028x.jfp-19-423.

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ABSTRACT The prevalence and diversity of Staphylococcus species from wild European rabbits (Oryctolagus cuniculus) in the Azores were investigated, and the antibiotic resistance phenotype and genotype of the isolates were determined. Nasal samples from 77 wild European rabbits from São Jorge and São Miguel islands in Azores were examined. Antibiotic susceptibility of the isolates was determined using the Kirby-Bauer disk diffusion method, and the presence of antimicrobial resistance genes and virulence factors was determined by PCR. The genetic lineages of S. aureus isolates were characteriz

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