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Journal articles on the topic 'Retina cDNA cloning/synthesis'

Author: Grafiati

Published: 4 June 2021

Last updated: 14 February 2022

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1

Voisin, P., J. Guerlotté, M. Bernard, J. P. Collin, and M. Cogné. "Molecular cloning and nucleotide sequence of a cDNA encoding hydroxyindole O-methyltransferase from chicken pineal gland." Biochemical Journal 282, no. 2 (1992): 571–76. http://dx.doi.org/10.1042/bj2820571.

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Hydroxyindole O-methyltransferase (EC 2.1.1.4) is the enzyme that catalyses the synthesis of melatonin in the pineal gland and in the retina. Polyadenylated RNA from chicken pineal glands was used to prepare a cDNA library in lambda gt11. The library was screened with an antiserum directed against chicken hydroxyindole O-methyltransferase, and one cDNA clone was isolated. The fusion protein expressed by phage lysogens was identified on Western blots as a 165 kDA immunoreactive protein (beta-galactosidase, 110 kDa; hydroxyindole O-methyltransferase, 38 kDa). The fusion protein exhibited hydroxy

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2

HSU, Lily C., Wen-Chung CHANG, Ines HOFFMANN, and Gregg DUESTER. "Molecular analysis of two closely related mouse aldehyde dehydrogenase genes: identification of a role for Aldh1, but not Aldh-pb, in the biosynthesis of retinoic acid." Biochemical Journal 339, no. 2 (1999): 387–95. http://dx.doi.org/10.1042/bj3390387.

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Mammalian class I aldehyde dehydrogenase (ALDH1) has been implicated as a retinal dehydrogenase in the biosynthesis of retinoic acid, a modulator of gene expression and cell differentiation. As the first step towards studying the regulation of ALDH1 and its physiological role in the biosynthesis of retinoic acid, mouse ALDH1 cDNA and genomic clones have been characterized. During the cloning process, an additional closely related gene was also isolated and named Aldh-pb, owing to its high amino acid sequence identity (92%) with the rat phenobarbitol-inducible ALDH protein (ALDH-PB). Aldh1 span

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3

Acharya, Harsha R., Constance M. Dooley, Wallace B. Thoreson, and Iqbal Ahmad. "cDNA Cloning and Expression Analysis ofNeuroDmRNA in Human Retina." Biochemical and Biophysical Research Communications 233, no. 2 (1997): 459–63. http://dx.doi.org/10.1006/bbrc.1997.6483.

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4

Nakagawa, Yuzo, Che-Hui Kuo, Kayoko Ishii, Sadao Shiosaka, Masaya Tohyama, and Naomasa Miki. "Cloning and characterization of a cDNA specific for bovine retina." Neuroscience Research 3, no. 4 (1986): 300–310. http://dx.doi.org/10.1016/0168-0102(86)90022-2.

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5

Yamaki, Kunihiko, Yoosuke Takahashi, Shozo Sakuragi, and Kenichi Matsubara. "Molecular cloning of the S-antigen cDNA from bovine retina." Biochemical and Biophysical Research Communications 142, no. 3 (1987): 904–10. http://dx.doi.org/10.1016/0006-291x(87)91499-9.

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6

Hughes, Bret A., Gyanendra Kumar, Yukun Yuan, et al. "Cloning and functional expression of human retinal Kir2.4, a pH-sensitive inwardly rectifying K+ channel." American Journal of Physiology-Cell Physiology 279, no. 3 (2000): C771—C784. http://dx.doi.org/10.1152/ajpcell.2000.279.3.c771.

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To identify novel potassium channel genes expressed in the retina, we screened a human retina cDNA library with an EST sequence showing partial homology to inwardly rectifying potassium (Kir) channel genes. The isolated cDNA yielded a 2,961-base pair sequence with the predicted open reading frame showing strong homology to the rat Kir2.4 (rKir2.4). Northern analysis of mRNA from human and bovine tissues showed preferential expression of Kir2.4 in the neural retina. In situ hybridization to sections of monkey retina detected Kir2.4 transcript in most retinal neurons. Somatic hybridization analy

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7

O'Brien, John, Harris Ripps, and Muayyad R. Al-Ubaidi. "Molecular cloning of a rod opsin cDNA from the skate retina." Gene 193, no. 2 (1997): 141–50. http://dx.doi.org/10.1016/s0378-1119(97)00079-6.

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8

Cauley, K., B. W. Agranoff, and D. Goldman. "Identification of a novel nicotinic acetylcholine receptor structural subunit expressed in goldfish retina." Journal of Cell Biology 108, no. 2 (1989): 637–45. http://dx.doi.org/10.1083/jcb.108.2.637.

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A new non-alpha (n alpha) member of the nicotinic acetylcholine receptor (nAChR) gene family designated GFn alpha-2 has been identified in goldfish retina by cDNA cloning. This cDNA clone encodes a protein with structural features common to all nAChR subunits sequenced to date; however, unlike all known alpha-subunits of the receptor, it lacks the cysteine residues believed to be involved in acetylcholine binding. Northern blot analysis shows multiple transcripts hybridizing to the GFn alpha-2 cDNA in goldfish retina but undetectable levels of hybridizable RNA in brain, muscle, or liver. S1 nu

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9

Pardhasaradhi, Komanduri, R. Krishnan Kutty, Chang Shin Park, and Gopal Krishna. "Cloning and sequencing of heat shock factor (HSF1) cDNA from human retina." Current Eye Research 13, no. 10 (1994): 739–42. http://dx.doi.org/10.3109/02713689409047008.

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10

ZOU, Ming-Feng, Lin BAI, Bo KANG, and Xue-Wei LI. "cDNA cloning and expression analysis of Opn4 gene in retina of swines." Hereditas (Beijing) 35, no. 7 (2013): 890–95. http://dx.doi.org/10.3724/sp.j.1005.2013.00890.

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11

Ishibashi, Kenichi, Sei Sasaki, and Fumiaki Marumo. "Molecular Cloning of a New Sodium Bicarbonate Cotransporter cDNA from Human Retina." Biochemical and Biophysical Research Communications 246, no. 2 (1998): 535–38. http://dx.doi.org/10.1006/bbrc.1998.8658.

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12

WAGNER, T. L. E., E. C. BEYER, and D. G. McMAHON. "Cloning and functional expression of a novel gap junction channel from the retina of Danio aquipinnatus." Visual Neuroscience 15, no. 6 (1998): 1137–44. http://dx.doi.org/10.1017/s0952523898156146.

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Electrical synapses, or gap junctions, are widely distributed in the vertebrate retina and are thought to play critical roles in the transmission and coding of visual signals. To investigate the molecular basis of this form of neural communication in the retina, we have isolated, characterized, and functionally expressed a cDNA for a gap junction channel derived from the retina of the teleost fish Danio aquipinnatus (giant danio). The cDNA contained an open reading frame of 1146 nucleotides encoding a connexin with a predicted molecular mass of 43.3 kDa which shared extensive identity with Rat

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13

Imamura, Yutaka, Ryo Kubota, Yimin Wang, et al. "Human Retina-Specific Amine Oxidase (RAO): cDNA Cloning, Tissue Expression, and Chromosomal Mapping." Genomics 40, no. 2 (1997): 277–83. http://dx.doi.org/10.1006/geno.1996.4570.

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14

Wiechmann, Allan F., and James A. Hammarback. "Molecular Cloning and Nucleotide Sequence of a cDNA Encoding Recoverin from Human Retina." Experimental Eye Research 56, no. 4 (1993): 463–70. http://dx.doi.org/10.1006/exer.1993.1059.

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15

Chai, Zhonglin, Phillip Brereton, Takashi Suzuki та ін. "17β-Hydroxysteroid Dehydrogenase Type XI Localizes to Human Steroidogenic Cells". Endocrinology 144, № 5 (2003): 2084–91. http://dx.doi.org/10.1210/en.2002-221030.

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We searched expressed sequence tag databases with conserved domains of the short-chain alcohol dehydrogenase superfamily and identified another isoform of 17β-hydroxysteroid dehydrogenase, 17βHSDXI. This enzyme converts 5α-androstane-3α, 17β-diol to androsterone. The substrate has been implicated in supporting gestation and modulating γ-aminobutyric acid receptor activity. 17βHSDXI is colinear with human retinal short-chain dehydrogenase/reductase retSDR2, a protein with no known biological activity (accession no. AAF06939). Of the proteins with known function, 17βHSDXI is most closely related

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16

Hara-Nishimura, Ikuko, Tetsunori Matsumoto, Hitoshi Mori, Mikio Nishimura, Reiko Hara, and Tomiyuki Hara. "Cloning and nucleotide sequence of cDNA for retinochrome, retinal photoisomerase from the squid retina." FEBS Letters 271, no. 1-2 (1990): 106–10. http://dx.doi.org/10.1016/0014-5793(90)80383-t.

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17

Taniguchi, Manabu, Shigetaka Yoshida, and Sadao Shiosaka. "813 Cloning of cDNA for a retina-specific serine protease and its mRNA expression." Neuroscience Research 25 (January 1996): S94. http://dx.doi.org/10.1016/0168-0102(96)88817-1.

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18

Dearry, Allen, Pierre Falardeau, Carol Shores, and Marc G. Caron. "D2 dopamine receptors in the human retina: Cloning of cDNA and localization of mRNA." Cellular and Molecular Neurobiology 11, no. 5 (1991): 437–53. http://dx.doi.org/10.1007/bf00734808.

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19

Krawinkel, Ulrich, and Rele Zoebelein. "Rapid synthesis of cDNA for cloning into lambda vectors." Nucleic Acids Research 14, no. 4 (1986): 1913. http://dx.doi.org/10.1093/nar/14.4.1913.

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20

Gonzalez-Fernandez, F., K. L. Kittredge, M. E. Rayborn, et al. "Interphotoreceptor retinoid-binding protein (IRBP), a major 124 kDa glycoprotein in the interphotoreceptor matrix of Xenopus laevis. Characterization, molecular cloning and biosynthesis." Journal of Cell Science 105, no. 1 (1993): 7–21. http://dx.doi.org/10.1242/jcs.105.1.7.

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We have demonstrated that the neural retina of Xenopus laevis secretes into the extracellular matrix surrounding the inner and outer segments of its photoreceptors a glycoprotein containing hydrophobic domains conserved in mammalian interphotoreceptor retinoid-binding proteins (IRBPs). The soluble extract of the interphotoreceptor matrix contains a 124 kDa protein that cross-reacts with anti-bovine IRBP immunoglobulins. In vitro [3H]fucose incorporation studies combined with in vivo light and electron microscopic autoradiographic analysis, showed that the IRBP-like glycoprotein is synthesized

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21

Schoen, T. J., K. Mazuruk, R. J. Waldbillig, et al. "Cloning and characterization of a chick embryo cDNA and gene for IGF-binding protein-2." Journal of Molecular Endocrinology 15, no. 1 (1995): 49–59. http://dx.doi.org/10.1677/jme.0.0150049.

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ABSTRACT We have isolated and characterized a cDNA for IGF-binding protein-2 (IGFBP-2) and its gene from the chick embryo. Using primers from a conserved region of the mammalian IGFBP-2 sequence, a cDNA clone (1·6 kb) was isolated from an embryonic day-18 chick retina cDNA library. Although the clone was truncated at the 5′ end, the complete coding sequence was obtained from 5′ rapid amplification of cDNA ends and genomic sequencing. The open reading frame encoded a 311 amino acid precursor protein which contains a putative 36 residue signal peptide. The mature 275 amino acid protein had a pre

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22

Arita, M., Y. Sato, A. Miyata та ін. "Human α-tocopherol transfer protein: cDNA cloning, expression and chromosomal localization". Biochemical Journal 306, № 2 (1995): 437–43. http://dx.doi.org/10.1042/bj3060437.

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alpha-Tocopherol transfer protein (alpha TTP), which specifically binds this vitamin and enhances its transfer between separate membranes, was previously isolated from rat liver cytosol. In the current study we demonstrated the presence of alpha TTP in human liver by isolating its cDNA from a human liver cDNA library. The cDNA for human alpha TTP predicts 278 amino acids with a calculated molecular mass of 31,749, and the sequence exhibits 94% similarity with rat alpha TTP at the amino acid level. The recombinant human alpha TTP expressed in Escherichia coli exhibits both alpha-tocopherol tran

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23

RYAN, JAMES C., SERGEY ZNOIKO, LIN XU, ROSALIE K. CROUCH, and JIAN-XING MA. "Salamander rods and cones contain distinct transducin alpha subunits." Visual Neuroscience 17, no. 6 (2000): 847–54. http://dx.doi.org/10.1017/s0952523800176047.

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The mammalian retina is known to contain two distinct transducins that interact with their respective rod and cone pigments. However, there are no reports of a nonmammalian species having two distinct transducins. In the present study, we report the cloning and cellular localization of two transducin α subunits (Gαt) from the tiger salamander. Through degenerate polymerase chain reaction (PCR) and subsequent screening of a salamander retina cDNA library, we have identified two forms of Gαt. When compared to existing sequences in GenBank, the cloned subunits showed high similarity to rod and co

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24

Hoe, Kwang-Lae, Ines Armando, Gustavo Baiardi, et al. "Molecular cloning, characterization, and distribution of the gerbil angiotensin II AT2 receptor." American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 285, no. 6 (2003): R1373—R1383. http://dx.doi.org/10.1152/ajpregu.00008.2003.

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We isolated a cDNA clone encoding the gerbil AT2 receptor (gAT2) gene from a gerbil adrenal gland cDNA library. The full-length cDNA contains a 1,089-bp open reading frame encoding 363 amino acid residues with 90.9, 96.1, and 95.6% identity with the human (hAT2), rat (rAT2), and mouse AT2 (mAT2) receptors, respectively. There are at least seven nonconserved amino acids in the NH2-terminal domain and in positions Val196, Val217, and Met293, important for angiotensin (ANG) II but not for CGP-42112 binding. Displacement studies in adrenal sections revealed that affinity of the gAT2 receptor was 1

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25

Taniguchi, Manabu, Shigetaka Yoshida, and Sadao Shiosaka. "715 cDNA cloning and expression of a splicing variant of MMet-1, a retina-specific serine protease." Neuroscience Research 28 (January 1997): S99. http://dx.doi.org/10.1016/s0168-0102(97)90259-5.

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26

Ogurusu, Tarou, Hideharu Taira та Ryuzo Shingai. "Identification of GABAA Receptor Subunits in Rat Retina: Cloning of the Rat GABAA Receptor ρ2-Subunit cDNA". Journal of Neurochemistry 65, № 3 (2002): 964–68. http://dx.doi.org/10.1046/j.1471-4159.1995.65030964.x.

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27

Revel, Frédérique, Jean-Paul Renard, and Véronique Duranthon. "PCR-generated cDNA libraries from reduced numbers of mouse oocytes." Zygote 3, no. 3 (1995): 241–50. http://dx.doi.org/10.1017/s096719940000263x.

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SummaryWe describe a rapid and reproducible method for cloning cDNA amplified from 10 mouse oocytes. The procedure consists in priming cDNA synthesis from a crude cellular extract using an oligo d(T) containing primer and submitting the size-limited cDNA first strand to poly(dG) tailing. The whole cDNA population is then polymerase chain reaction (PCR) amplified using two primers complementary to oligo d(A) and oligo d(G) ends of the cDNA. In this procedure no purification steps are required. We obtained about 5 ×106 clones from 10 oocytes. Screening of the library showed that the relative abu

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28

SCHNELL, Danny J., Danny C. ALEXANDER, Bill G. WILLIAMS, and Marilynn E. ETZLER. "cDNA cloning and in vitro synthesis of the Dolichos biflorus seed lectin." European Journal of Biochemistry 167, no. 2 (1987): 227–31. http://dx.doi.org/10.1111/j.1432-1033.1987.tb13327.x.

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29

Redpath, Nicholas T., Nigel T. Price, and Christopher G. Proud. "Cloning and Expression of cDNA Encoding Protein Synthesis Elongation Factor-2 Kinase." Journal of Biological Chemistry 271, no. 29 (1996): 17547–54. http://dx.doi.org/10.1074/jbc.271.29.17547.

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30

Altmann, M., C. Handschin, and H. Trachsel. "mRNA cap-binding protein: cloning of the gene encoding protein synthesis initiation factor eIF-4E from Saccharomyces cerevisiae." Molecular and Cellular Biology 7, no. 3 (1987): 998–1003. http://dx.doi.org/10.1128/mcb.7.3.998.

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We have isolated genomic and cDNA clones encoding protein synthesis initiation factor eIF-4E (mRNA cap-binding protein) of the yeast Saccharomyces cerevisiae. Their identity was established by expression of a cDNA in Escherichia coli. This cDNA encodes a protein indistinguishable from purified eIF-4E in terms of molecular weight, binding to and elution from m7GDP-agarose affinity columns, and proteolytic peptide pattern. The eIF-4E gene was isolated by hybridization of cDNA to clones of a yeast genomic library. The gene lacks introns, is present in one copy per haploid genome, and encodes a pr

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31

Altmann, M., C. Handschin, and H. Trachsel. "mRNA cap-binding protein: cloning of the gene encoding protein synthesis initiation factor eIF-4E from Saccharomyces cerevisiae." Molecular and Cellular Biology 7, no. 3 (1987): 998–1003. http://dx.doi.org/10.1128/mcb.7.3.998-1003.1987.

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We have isolated genomic and cDNA clones encoding protein synthesis initiation factor eIF-4E (mRNA cap-binding protein) of the yeast Saccharomyces cerevisiae. Their identity was established by expression of a cDNA in Escherichia coli. This cDNA encodes a protein indistinguishable from purified eIF-4E in terms of molecular weight, binding to and elution from m7GDP-agarose affinity columns, and proteolytic peptide pattern. The eIF-4E gene was isolated by hybridization of cDNA to clones of a yeast genomic library. The gene lacks introns, is present in one copy per haploid genome, and encodes a pr

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32

YOSHIDA, Saishu, Hiroki UEHARU, Masashi HIGUCHI, et al. "Molecular Cloning of Rat and Porcine Retina-derived POU Domain Factor 1 (POU6F2) from a Pituitary cDNA Library." Journal of Reproduction and Development 60, no. 4 (2014): 288–94. http://dx.doi.org/10.1262/jrd.2014-023.

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33

Ryba, N. J. P., J. B. C. Findlay та J. D. Reid. "The molecular cloning of the squid (Loligo forbesi) visual Gq-α subunit and its expression in Saccharomyces cerevisiae". Biochemical Journal 292, № 2 (1993): 333–41. http://dx.doi.org/10.1042/bj2920333.

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The sequence of the alpha-subunit of the major G-protein from the squid (Loligo forbesi) retina was predicted from its cDNA to be a member of the Gq subclass. The abundance of the squid Gq-alpha in the squid photoreceptor membranes suggests that the protein functions in phototransduction; the sequence of this G-protein is consistent with it mediating the light-dependent activation of a phospholipase C. The squid G-alpha was expressed in the yeast Saccharomyces cerevisiae, where it was unable to replace the function of GPA1, the yeast G-alpha homologue that regulates the mating response, sugges

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34

van Hofsten, P., I. Faye, K. Kockum, et al. "Molecular cloning, cDNA sequencing, and chemical synthesis of cecropin B from Hyalophora cecropia." Proceedings of the National Academy of Sciences 82, no. 8 (1985): 2240–43. http://dx.doi.org/10.1073/pnas.82.8.2240.

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35

Cutting, G. R., L. Lu, B. F. O'Hara, et al. "Cloning of the gamma-aminobutyric acid (GABA) rho 1 cDNA: a GABA receptor subunit highly expressed in the retina." Proceedings of the National Academy of Sciences 88, no. 7 (1991): 2673–77. http://dx.doi.org/10.1073/pnas.88.7.2673.

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36

GOLDMAN, Daniel, Mohan K. SAPRU, Scott STEWART, et al. "Cloning and characterization of GETS-1, a goldfish Ets family member that functions as a transcriptional repressor in muscle." Biochemical Journal 335, no. 2 (1998): 267–75. http://dx.doi.org/10.1042/bj3350267.

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An Ets transcription factor family member, GETS-1, was cloned from a goldfish retina cDNA library. GETS-1 contains a conserved Ets DNA-binding domain at its N-terminus and is most similar to ternary complex factor (TCF) serum-response-factor protein-1a (SAP-1a). GETS-1 is expressed in many tissues, but is enriched in retina and brain. As with the TCFs SAP-1a and ets-related protein (ERP), overexpression of the GETS-1 promoter suppresses nicotinic acetylcholine receptor ε-subunit gene expression in cultured muscle cells. A consensus Ets binding site sequence in the promoter of the ε-subunit gen

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37

Tamura, K., H. Nishiura, J. Mori, and H. Imai. "Cloning and characterization of a cDNA encoding serine palmitoyltransferase in Arabidopsis thaliana." Biochemical Society Transactions 28, no. 6 (2000): 745–47. http://dx.doi.org/10.1042/bst0280745.

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The first and committed step in de novo sphingo-lipid synthesis is catalysed by serine palmitoyl-transferase (EC 2.3.1.50), which condenses serine and palmitoyl-CoA to form 3-ketosphinganine in a pyridoxal-5Î-phosphate-dependent reaction. We have isolated and characterized a cDNA clone from Arabidopsis thaliana that is homologous to yeast and mammalian LCB2. For a functional identification, the A. thaliana homologous cDNA was expressed in Escherichia coli which resulted in significant production of new sphinganine in E. coli cells.

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38

Bryndová, J., P. Klusoňová, M. Kučka, K. Mazancová-Vagnerová, I. Mikšík, and J. Pácha. "Cloning and expression of chicken 20-hydroxysteroid dehydrogenase." Journal of Molecular Endocrinology 37, no. 3 (2006): 453–62. http://dx.doi.org/10.1677/jme.1.02025.

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The ligand specificity and activation of steroid receptors depend considerably on the enzymatic activities involved in local pre-receptor synthesis and the metabolism of the steroids. Several enzymes in particular, steroid dehydrogenases have been shown to participate in this process. Here we report the isolation of 20-hydroxysteroid dehydrogenase (ch20HSD) cDNA from chicken intestine and the distribution of ch20HSD mRNA and 20-reductase activity in various avian tissues. Using a reverse transcription PCR and comparison with the known sequences of mammalian 20βHSDs, we have isolated a new ch20

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Bellemare, Guy, Claude Potvin, Claire Simard, and Lucie Larouche. "Use of a phage vector for rapid synthesis and cloning of single-stranded cDNA." Gene 52, no. 1 (1987): 11–19. http://dx.doi.org/10.1016/0378-1119(87)90390-8.

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40

Rocher, Corinne, Chi Faucheu, Françoise Hervé, Claude Bénicourt, and Jean-Louis Lalanne. "Cloning of murine SeGpx cDNA and synthesis of mutated GPx proteins in Escherichia coli." Gene 98, no. 2 (1991): 193–200. http://dx.doi.org/10.1016/0378-1119(91)90173-9.

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41

Sheffield, WP, AB Brothers, MJ Wells, MW Hatton, BJ Clarke, and MA Blajchman. "Molecular cloning and expression of rabbit antithrombin III." Blood 79, no. 9 (1992): 2330–39. http://dx.doi.org/10.1182/blood.v79.9.2330.2330.

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Abstract A cDNA containing the complete open-reading frame encoding rabbit antithrombin III (AT-III) was isolated from a rabbit liver cDNA expression library, using a specific antibody as a probe. Sequence analysis showed 84% identity between the deduced amino acid sequences of the rabbit and human proteins. A previously described cell-free expression system was used to verify the identity of the clone. The full-length cDNA was inserted into an expression vector, and messenger RNA (mRNA) transcripts generated. In vitro translation of these transcripts, in the presence of [35S]methionine, in an

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42

Sheffield, WP, AB Brothers, MJ Wells, MW Hatton, BJ Clarke, and MA Blajchman. "Molecular cloning and expression of rabbit antithrombin III." Blood 79, no. 9 (1992): 2330–39. http://dx.doi.org/10.1182/blood.v79.9.2330.bloodjournal7992330.

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A cDNA containing the complete open-reading frame encoding rabbit antithrombin III (AT-III) was isolated from a rabbit liver cDNA expression library, using a specific antibody as a probe. Sequence analysis showed 84% identity between the deduced amino acid sequences of the rabbit and human proteins. A previously described cell-free expression system was used to verify the identity of the clone. The full-length cDNA was inserted into an expression vector, and messenger RNA (mRNA) transcripts generated. In vitro translation of these transcripts, in the presence of [35S]methionine, in an mRNA-dep

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43

Ding, C., E. D. Potter, W. Qiu, S. L. Coon, M. A. Levine, and S. E. Guggino. "Cloning and widespread distribution of the rat rod-type cyclic nucleotide-gated cation channel." American Journal of Physiology-Cell Physiology 272, no. 4 (1997): C1335—C1344. http://dx.doi.org/10.1152/ajpcell.1997.272.4.c1335.

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We used Northern blot analysis, ribonuclease protection assay (RPA), reverse transcriptase-polymerase chain reaction, and in situ hybridization to investigate the hypothesis that the CNG1 isoform of the cyclic nucleotide-gated nonselective cation channel may be widely distributed in tissues of the rat. A cDNA encoding the CNG1 isoform was isolated from rat eye and human retina, and partial sequences were isolated from rat pineal gland and human kidney. Northern blot analysis revealed a 3.1-kilobase (kb) CNG1 transcript in rat eye, pineal gland, pituitary, adrenal gland, and spleen, and a large

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44

BLADERGROEN, Bellinda A., Martin HOUWELING, Math J. H. GEELEN, and Lambert M. G. VAN GOLDE. "Cloning and expression of CTP:phosphoethanolamine cytidylyltransferase cDNA from rat liver." Biochemical Journal 343, no. 1 (1999): 107–14. http://dx.doi.org/10.1042/bj3430107.

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CTP:phosphoethanolamine cytidylyltransferase (ET) is a key regulatory enzyme in the CDP-ethanolamine pathway for phosphatidylethanolamine synthesis. As a first step in the elucidation of the structure-function relationship and the regulation of ET, an ET cDNA was cloned from rat liver. The cloned cDNA encodes a protein of 404 amino acid residues with a calculated molecular mass of 45.2 kDa. The deduced amino acid sequence is very similar to that of human ET (89% identity). Furthermore, it shows less, but significant, similarity to yeast ET as well as to other cytidylyltransferases, including r

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45

Kylmä, Tarja, Lars Paulin, Mary Y. Hurwitz, Richard L. Hurwitz та Bertel Kommonen. "Cloning and analysis of the cDNA encoding the rod G-protein transducin α, β1 and γ1 subunits from the canine retina". Gene 193, № 1 (1997): 1–4. http://dx.doi.org/10.1016/s0378-1119(97)00060-7.

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Lu, J., S. B. Laursen, S. Thiel, J. C. Jensenius, and K. B. M. Reid. "The cDNA cloning of conglutinin and identification of liver as a primary site of synthesis of conglutinin in members of the Bovidae." Biochemical Journal 292, no. 1 (1993): 157–62. http://dx.doi.org/10.1042/bj2920157.

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Bovine conglutinin is a collagen-like, C-type, plasma lectin which belongs to the group of proteins called ‘collectins’. Two inosine-containing oligonucleotides were synthesized, based on the published protein sequence for bovine conglutinin [Lee, Leiby, Allar, Paris, Lerch and Okarma (1991) J. Biol. Chem. 266, 2715-2723], and PCR on target DNA from a bovine liver lambda gt 11 cDNA library yielded a product of the expected size of 210 bp. Screening of the library with this cDNA fragment identified a single positive clone, with an insert of 0.9 kb, coding for bovine conglutinin from residue 70

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Yu, H. H., and S. M. Wong. "Synthesis of biologically active cDNA clones of cymbidium mosaic potexvirus using a population cloning strategy." Archives of Virology 143, no. 8 (1998): 1617–20. http://dx.doi.org/10.1007/s007050050402.

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Porrello, K., S. P. Bhat, and D. Bok. "Detection of interphotoreceptor retinoid binding protein (IRBP) mRNA in human and cone-dominant squirrel retinas by in situ hybridization." Journal of Histochemistry & Cytochemistry 39, no. 2 (1991): 171–76. http://dx.doi.org/10.1177/39.2.1987260.

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Interphotoreceptor retinoid binding protein (IRBP) is a soluble glycolipoprotein located between the neurosensory retina and pigment epithelium, which may serve to transport vitamin A derivatives between these tissues. The specific cell type responsible for IRBP synthesis has not been well established. To address this issue, we have examined the expression of IRBP mRNA in human and cone-dominant ground squirrel retinas by in situ hybridization. Optimal labeling and histological resolution were achieved with 35S- and 3H-labeled anti-sense riboprobes made from a human IRBP cDNA clone, and semi-t

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Wang, R. F., P. F. Robbins, Y. Kawakami, X. Q. Kang, and S. A. Rosenberg. "Identification of a gene encoding a melanoma tumor antigen recognized by HLA-A31-restricted tumor-infiltrating lymphocytes." Journal of Experimental Medicine 181, no. 2 (1995): 799–804. http://dx.doi.org/10.1084/jem.181.2.799.

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The availability of antitumor cytotoxic T lymphocytes which can be generated from either peripheral blood lymphocytes after stimulation in vitro or tumor infiltrating lymphocytes (TIL) has made it possible to identify a number of melanoma antigens presented by major histocompatibility complex class I molecules. The present and previous studies indicated that TIL586 recognized an antigen expressed on most melanoma and normal melanocytes in the context of the HLA-A31 molecule. We report here the cloning of a cDNA that directs the expression of the shared melanoma antigen recognized by this TIL.

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Takahashi, N., K. Yoshihama, S. Kikuyama, K. Yamamoto, K. Wakabayashi, and Y. Kato. "Molecular cloning and nucleotide sequence analysis of complementary DNA for bullfrog prolactin." Journal of Molecular Endocrinology 5, no. 3 (1990): 281–87. http://dx.doi.org/10.1677/jme.0.0050281.

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ABSTRACT A prolactin cDNA was cloned from a cDNA expression library constructed from total RNA of bullfrog (Rana catesbeiana) adenohypophyses by immunoscreening with antiserum against bullfrog prolactin. The cDNA clone thus obtained contained a 249 bp insert. Using this clone as a probe, plaque hybridizations were performed and two additional clones obtained. These clones had a polyadenylation site different from that of the first obtained clone, suggesting that the 3′-untranslated sequence was heterogeneous in length. The longest clone contained 830 bp, which encoded part of the signal peptid

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