Academic literature on the topic 'Retroviral gene expression'

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Journal articles on the topic "Retroviral gene expression"

1

Tolstoshev, Paul, and W. French Anderson. "Gene expression using retroviral vectors." Current Opinion in Biotechnology 1, no. 1 (1990): 55–61. http://dx.doi.org/10.1016/0958-1669(90)90010-i.

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2

Blø, Magnus, David R. Micklem, and James B. Lorens. "Enhanced gene expression from retroviral vectors." BMC Biotechnology 8, no. 1 (2008): 19. http://dx.doi.org/10.1186/1472-6750-8-19.

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3

Smith, Michael J., Scott D. Gitlin, Catherine M. Browning, et al. "GLI-2 Modulates Retroviral Gene Expression." Journal of Virology 75, no. 5 (2001): 2301–13. http://dx.doi.org/10.1128/jvi.75.5.2301-2313.2001.

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ABSTRACT GLI proteins are involved in the development of mice, humans, zebrafish, Caenorhabditis elegans, Xenopus, andDrosophila. While these zinc finger-containing proteins bind to TG-rich promoter elements and are known to regulate gene expression in C. elegans and Drosophila, mechanistic understanding of how regulation is mediated through naturally occurring transcriptional promoters is lacking. One isoform of human GLI-2 appears to be identical to a factor previously called Tax helper protein (THP), thus named due to its ability to interact with a TG-rich element in the human T-lymphotropi
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4

Hao, De-Long, Chang-Mei Liu, Wen-Ji Dong, et al. "Knockdown of Human p53 Gene Expression in 293-T Cells by Retroviral Vector-mediated Short Hairpin RNA." Acta Biochimica et Biophysica Sinica 37, no. 11 (2005): 779–83. http://dx.doi.org/10.1111/j.1745-7270.2005.00107.x.

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Abstract RNA interference (RNAi) is an evolutionarily conserved process of gene silencing in multiple organisms, which has become a powerful tool for investigating gene function by reverse genetics. Recently, many groups have reported to use synthesized oligonucleotides or siRNA encoding plasmids to induce RNAi in mammalian cells by transfection, but this is still limited in its application, especially when it is necessary to generate long-term gene silencing in vivo. To circumvent this problem, retrovirus- or lentivirus-delivered RNAi has been developed. Here, we described two retroviral syst
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5

Grabarczyk, Piotr, Piotr J. Wysocki, Katarzyna Gryska, and Andrzej Mackiewicz. "Expression of PiT1 and PiT2 retroviral receptors and transduction efficiency of tumor cells." Acta Biochimica Polonica 49, no. 2 (2002): 333–39. http://dx.doi.org/10.18388/abp.2002_3791.

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Recombinant retroviral vectors are still the most common gene delivery vehicles for gene therapy purposes, especially for construction of genetically modified tumor vaccines (GMTV). However, these vehicles are characterized by relatively low titre and in the case of many tumor cell lines, low transduction efficiency. We constructed bicistronic retroviral vector pseudotypes of amphotropic murine leukemia virus (A-MuLV) and gibbon ape leukemia virus (GaLV), encoding enhanced green fluorescent protein (EGFP) as a rapid and easily detectable reporter gene. Transduction of five different human mela
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6

Agarwal, Manju, Timothy W. Austin, Franck Morel, Jingyi Chen, Ernst Böhnlein, and Ivan Plavec. "Scaffold Attachment Region-Mediated Enhancement of Retroviral Vector Expression in Primary T Cells." Journal of Virology 72, no. 5 (1998): 3720–28. http://dx.doi.org/10.1128/jvi.72.5.3720-3728.1998.

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ABSTRACT We have studied retroviral transgene expression in primary human lymphocytes. Our data demonstrate that transgene expression is high in activated primary CD4+ T cells but significantly decreased in mitotically quiescent cells. Incorporation of a DNA fragment from the scaffold attachment region (SAR) of the human beta interferon gene into the vector improved transgene expression, particularly in quiescent cells. The SAR element functioned in an orientation-dependent manner and enhanced expression of Moloney murine leukemia virus- and murine embryonic stem cell-based vectors. Clonal ana
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7

Richardson, C., M. Ward, S. Podda, and A. Bank. "Mouse fetal liver cells lack functional amphotropic retroviral receptors." Blood 84, no. 2 (1994): 433–39. http://dx.doi.org/10.1182/blood.v84.2.433.bloodjournal842433.

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We have been transducing mouse hematopoietic cells with the human MDR1 (MDR) gene in retroviral vectors to determine the optimal conditions for retroviral gene transfer as a model system for potential human gene therapy. In these studies, we have demonstrated transduction and expression of the human MDR gene using ecotropic and amphotropic MDR- retroviral producer lines. To obtain more mouse hematopoietic cells for detailed study, mouse fetal liver cells (FLC) have been used for MDR transduction and expression, and to reconstitute the ablated marrows of live adult mice. FLC contain hematopoiet
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8

Antoniou, Michael N., Kristian Alsbjerg Skipper, and Omer Anakok. "Optimizing Retroviral Gene Expression for Effective Therapies." Human Gene Therapy 24, no. 4 (2013): 363–74. http://dx.doi.org/10.1089/hum.2013.062.

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9

Yee, J. K., J. C. Moores, D. J. Jolly, J. A. Wolff, J. G. Respess, and T. Friedmann. "Gene expression from transcriptionally disabled retroviral vectors." Proceedings of the National Academy of Sciences 84, no. 15 (1987): 5197–201. http://dx.doi.org/10.1073/pnas.84.15.5197.

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10

Anderson, Claire L., and Gwyn T. Williams. "Apoptosis Gene Hunting Using Retroviral Expression Cloning." Scientific World JOURNAL 1 (2001): 33. http://dx.doi.org/10.1100/tsw.2001.154.

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